CN107802025A - A kind of preparation method of ginkgo biloba p.e and its application in cigarette - Google Patents
A kind of preparation method of ginkgo biloba p.e and its application in cigarette Download PDFInfo
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- CN107802025A CN107802025A CN201711273705.0A CN201711273705A CN107802025A CN 107802025 A CN107802025 A CN 107802025A CN 201711273705 A CN201711273705 A CN 201711273705A CN 107802025 A CN107802025 A CN 107802025A
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- ginkgo biloba
- cigarette
- ginkgo
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- ethanol
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- 235000008100 Ginkgo biloba Nutrition 0.000 title claims abstract description 100
- 244000194101 Ginkgo biloba Species 0.000 title claims abstract description 95
- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 50
- 235000011201 Ginkgo Nutrition 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000001914 filtration Methods 0.000 claims abstract description 19
- 239000000654 additive Substances 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 230000000996 additive effect Effects 0.000 claims abstract description 10
- 239000003124 biologic agent Substances 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 238000010992 reflux Methods 0.000 claims abstract description 7
- 241000218628 Ginkgo Species 0.000 claims abstract 7
- 241000208125 Nicotiana Species 0.000 claims description 16
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 244000063299 Bacillus subtilis Species 0.000 claims description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 11
- 108010059892 Cellulase Proteins 0.000 claims description 10
- 108010047754 beta-Glucosidase Proteins 0.000 claims description 10
- 102000006995 beta-Glucosidase Human genes 0.000 claims description 10
- 229940106157 cellulase Drugs 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 230000008020 evaporation Effects 0.000 claims description 3
- 238000013329 compounding Methods 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 241000726221 Gemma Species 0.000 claims 1
- 239000003546 flue gas Substances 0.000 abstract description 11
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 abstract description 9
- -1 hydrolysed ferment Substances 0.000 abstract description 6
- 210000000214 mouth Anatomy 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 235000008216 herbs Nutrition 0.000 abstract description 4
- 239000002304 perfume Substances 0.000 abstract description 4
- 239000000796 flavoring agent Substances 0.000 abstract description 3
- 235000019634 flavors Nutrition 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 9
- 150000003254 radicals Chemical class 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000001953 sensory effect Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- MOLPUWBMSBJXER-YDGSQGCISA-N bilobalide Chemical compound O([C@H]1OC2=O)C(=O)[C@H](O)[C@@]11[C@@](C(C)(C)C)(O)C[C@H]3[C@@]21CC(=O)O3 MOLPUWBMSBJXER-YDGSQGCISA-N 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000000391 smoking effect Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- OVSQVDMCBVZWGM-LQSBFMDOSA-N 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[(2r,3s,4r,5r,6s)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-LQSBFMDOSA-N 0.000 description 1
- 241000282452 Ailuropoda melanoleuca Species 0.000 description 1
- 241000722948 Apocynum cannabinum Species 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 1
- 238000004435 EPR spectroscopy Methods 0.000 description 1
- 241000218791 Ginkgoaceae Species 0.000 description 1
- 239000004378 Glycyrrhizin Substances 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical group 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229930184727 ginkgolide Natural products 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000012680 lutein Nutrition 0.000 description 1
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 1
- 229960005375 lutein Drugs 0.000 description 1
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 description 1
- 239000001656 lutein Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 230000014860 sensory perception of taste Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 1
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0003—Compounds of unspecified constitution defined by the chemical reaction for their preparation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Application the invention provides a kind of preparation method of ginkgo biloba p.e and its in cigarette, comprises the following steps：(1) cleaning of fresh ginkgo leaf is drained into cut into serving pieces, is extracted using ethanol solution microwave abstracting, filtering and collecting filter liquid, obtain ginkgo biloba succi A.(2) the ginkgo leaf residue in collection step (1), add water and biological agent, hydrolysed ferment, ethanol constant temperature refluxing extraction is added into hydrolysed ferment liquid, ginkgo biloba succi B is obtained after filtering.(3) after ginkgo biloba succi A and ginkgo biloba succi B is merged, ginkgo biloba p.e is obtained after being concentrated under reduced pressure.Cigarette ginkgo biloba p.e prepared by the present invention, with the distinctive medicinal herbs odor characteristic of ginkgo leaf, as additive application in cigarette, coordinate with cigarette perfume, abundant cigarette flavor amount, efficiently solve conventional extracting technology and easily bring the negative issue that excitant is big, oral cavity residual is more, have the function that to remove free-radical contents in cigarette mainstream flue gas.
Description
Technical field
The present invention relates to cigarette flavors field, the preparation method of specifically a kind of ginkgo biloba p.e and its
Application in cigarette.
Background technology
Ginkgo (also known as Gong Sunshu, gingko) is Gymnospermae Ginkgoaceae plant, is seeds most ancient on the current earth
One of.Ginkgo has the laudatory title of " giant panda of plant kingdom ", is to study " living fossil " that ancient times earth geology becomes deeply, is arranged by country
For two level rare and protected trees.China's gingko resource owning amount accounts for 75% or so of world's total amount, occupies first place in the world.Domestic big portion
The ginkgo of region-by-region is artificial cultivation, is mainly distributed on the provinces and cities such as Hubei, Zhejiang, Anhui, Fujian, Henan, Sichuan.Suizhou, hubei
Thousands of ancient ginkgo are grown among the remote mountains of one band, are that the big intensive sheet of ancient Ginkgo population in the world four falls it
One.
The free radical of high concentration can be produced during cigarette burning, these free radicals can be attacked directly or indirectly, damaging cells
With the material such as enzyme, mutation is produced with DNA with reference to cell transformation is made.Therefore research flue gas free radical, high effect nontoxic oneself is developed
By base scavenger, for reducing smoking property of cigarette harm, alleviate the tool of the contradiction between smoking and health and be of great significance.Mesh
Before, using plant additive, the research of flue gas free radical is many reducing, the bluish dogbane such as reported, Tea Polyphenols, glycyrrhizin,
Lutein etc. all has the effect of drop free radical as cigarette additive, and it is 7%-20% or so that it, which reduces amplitude,.These plants
The form that the adding technology of additive is all made as microcapsules mostly is added in filter tip, complex process.This is primarily due to, first
First, most plant additives are all liposoluble, are sprayed directly in pipe tobacco and are had any problem in technique；Secondly these plants addition
Agent is added in pipe tobacco, can change the suction taste of cigarette, or even brings very deleterious effect.The natural plants of free radical can be reduced
Have a lot, but cigarette free radical can be reduced simultaneously, and can brings the seldom of good positive effect to cigarette odor-absorbing.At present
Although the natural plants for the drop cigarette free radical reported are a lot, really use it for industrialized production, bring direct show
Almost not having but for economic benefit is write, its key reason is exactly can not solve negative effect of the plant additive to cigarette odor-absorbing.
Ginkgo leaf has multiple biological activities and medical value, such as anti-oxidant, removing human body is certainly as a kind of Chinese herbal medicine
By base, improve blood flow, increase brain blood supply and other effects.Ginkgo leaf primary medicinal component is flavonoids and lactone compound
(content is more than 24.0%), the chromocor compound having now been found that has rutin, the Radix Astragali, isoquercitin, kaempferol etc., lactone
Compound has ginkgolides and Bilobalide, can assign the medicinal herbs smell of uniqueness, has the prospect as essence spice for cigarette.
In recent years, effect of the natural plant pefume in terms of improving cigarette comfortableness and highlighting cigarette style characteristic obtains
Tobacco scientific and technical personnel and consumer's is widely recognized as.Ginkgo leaf is used for medicine more in the achievement in research of documents and materials report at present
Field.The present inventor has found in research process:Simply by the ginkgo biloba p.e obtained by extraction technique, due to
Extraction temperature is higher, and oxidation Decomposition easily occurs for part ketone, esters effumability composition, causes fragrance loss, the spy of product
Fragrance unobvious are levied, naturally feel not ideal enough, is added in cigarette and easily brings the negative works such as excitant is big, oral cavity residual is more
With the requirement as additive of tobacco product can not be met.
The content of the invention
The purpose of the present invention is according to the deficiencies in the prior art, there is provided a kind of preparation method of ginkgo biloba p.e and its
Application in cigarette, extract prepared by the present invention have the distinctive medicinal herbs odor characteristic of ginkgo leaf, and note is natural, coordinates, fragrant
The natural sense of taste is stronger, and as additive application in cigarette, personalized note protrudes, and coordinates with cigarette perfume, enriches cigarette
Perfume quantity, aroma quality is lifted, pleasant impression is comfortable, and oral cavity is clean, the soft exquisiteness of flue gas, efficiently solves the easy band of conventional extracting technology
Carry out the negative issue that excitant is big, oral cavity residual is more, while free-radical contents in cigarette mainstream flue gas can be removed.
To achieve the above object, a kind of preparation method of ginkgo biloba p.e provided by the invention and its answering in cigarette
With it comprises the following steps：
(1) after the cleaning of fresh ginkgo leaf is drained away the water, cut into serving pieces, then by the second of sheet ginkgo leaf and 30-60%
Alcoholic solution is according to 1:After 5-10 mass ratio mixing, it is put into microwave abstracting equipment and is extracted, microwave power is set to 350~
700W, system temperature is set in 10~30min rise to 50~65 DEG C from room temperature, and after handling 0.5h~1h, filter is collected after filtering
Liquid, obtain ginkgo biloba succi A.
(2) the ginkgo leaf residue in collection step (1), the water of 2-5 times of quality is added, adds 0.1-0.5% mass ratios
Biological agent, after stirring, under the conditions of pH4-6,30-50 DEG C of temperature incubate hydrolysed ferment 6-18h, obtain hydrolysed ferment
Liquid.The ethanol of food-grade 95% (alcohol concentration is 30-60% in system) is added into hydrolysed ferment liquid, in 60-80 DEG C of thermostatted water
Bathe refluxing extraction 1-3 times, merging filtrate after filtering, obtain ginkgo biloba succi B.
(3) after ginkgo biloba succi A and ginkgo biloba succi B is merged, it is concentrated under reduced pressure as the 1/4 of original volume.To system
It is middle to add 5-8 times of water, after stirring, standing 6-12h, the 1/5-1/3 that original volume is concentrated under reduced pressure into after supernatant liquid filtering is taken,
Ginkgo biloba p.e as of the present invention.
The fresh ginkgo leaf derives from Suizhou, hubei Luoyang town ginkgo paddy.
Biological agent in the step (2) refers to beta-glucosidase, cellulase, live bacillus subtilis preparation
In one or more.
Biological agent in the step (2) for two or more compounding when, beta-glucosidase, cellulase,
The compound proportion of live bacillus subtilis preparation is 1-2：1-2：0.2-1.
The enzyme activity of the beta-glucosidase and cellulase is the u/g of 5-20 ten thousand, is contained in live bacillus subtilis preparation
Bacillus subtilis viable count is not less than 1.0*108CFU/g。
The present invention provides another technical solution：A kind of ginkgo biloba p.e prepared using the above method is answered
With, it is characterised in that：The ginkgo biloba p.e is added in cigarette as natural tobacco additive, and adding proportion is pipe tobacco matter
The 0.1%-0.3% of amount.
The ginkgo biloba p.e adds concretely comprising the following steps for cigarette shreds：Ginkgo biloba p.e is dissolved in into mass concentration is
In 70% ethanol, it will be sprayed at dissolved with the alcohol solvent of ginkgo biloba p.e in cigarette produces coiling process on pipe tobacco, with
Evaporation solvent afterwards.
The advantage of the invention is that：
(1) commercially available ginkgo leaf is generally dried Chinese medicine, and ginkgo leaf is often aromatic in drying, concocting process
Composition volatilization loss, the present invention have certain raw material advantage from fresh ginkgo leaf as extraction raw material.
(2) microwave extraction process has the high technical advantage of low temperature extraction, extracting efficiency, and it is easy to reduce ketone, esters etc.
The oxidation Decomposition of volatile fragrant components, effectively prevent fragrance loss.
(3) fermentation is hydrolyzed to ginkgo leaf residue using biological agent in the present invention, and macromolecular substances are degraded into can profit
Small-molecule substance, and the organic acids such as lactic acid are produced, the comprehensive utilization ratio of raw material is improved, avoids the wasting of resources.
(4) extract prepared by the present invention has the distinctive medicinal herbs odor characteristic of ginkgo leaf, and note is natural, coordinates, fragrance
Natural sense it is stronger, as additive application in cigarette, personalized note protrudes, and coordinates with cigarette perfume, and it is fragrant to enrich cigarette
Tolerance, aroma quality is lifted, pleasant impression is comfortable, and oral cavity is clean, the soft exquisiteness of flue gas, efficiently solves conventional extracting technology and easily brings
Excitant is big, oral cavity remains more negative issue, while has the function that to remove free-radical contents in cigarette mainstream flue gas.
(5) present invention has the characteristics that simple to operate, easily controllable, cost of material is low, is suitable for actual production conversion, fits
Cooperate to carry out popularization and application for a kind of method for preparing tobacco aromaticss.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment one
(1) by fresh ginkgo leaf cleaning drain away the water after, cut into serving pieces, then by sheet ginkgo leaf with 60% ethanol it is molten
Liquid is according to 1:After 10 ratio mixing, it is put into microwave abstracting equipment and is extracted, microwave power is set to 550W, and system temperature is set
To rise to 50~65 DEG C from room temperature in 10~30min, after handling 0.5h, filtering and collecting filter liquid, ginkgo biloba succi is obtained
A。
(2) the ginkgo leaf residue in collection step (1), the water of 5 times of quality is added, adds the biology of 0.5% mass ratio
(beta-glucosidase, cellulase, the compound proportion of live bacillus subtilis preparation are 1 to preparation：1：0.5), stir
Afterwards, hydrolysed ferment 6h is incubated under the conditions of pH4,30 DEG C of temperature, obtains hydrolysed ferment liquid.Food-grade is added into hydrolysed ferment liquid
95% ethanol (in system alcohol concentration be 50%), in 60 DEG C of water bath with thermostatic control refluxing extractions 3 times, merging filtrate after filtering, obtain
Ginkgo biloba succi B.
(3) after ginkgo biloba succi A and ginkgo biloba succi B is merged, it is concentrated under reduced pressure as the 1/4 of original volume.To system
The middle water for adding 8 times, after stirring, 10h is stood, the 1/3 of original volume is concentrated under reduced pressure into after taking supernatant liquid filtering, be this hair
Bright described ginkgo biloba p.e.
Embodiment two
(1) by fresh ginkgo leaf cleaning drain away the water after, cut into serving pieces, then by sheet ginkgo leaf with 30% ethanol it is molten
Liquid is according to 1:After 8 ratio mixing, it is put into microwave abstracting equipment and is extracted, microwave power is set to 350W, and system temperature is set
To rise to 50~65 DEG C from room temperature in 10~30min, after handling 1h, filtering and collecting filter liquid, ginkgo biloba succi A is obtained.
(2) the ginkgo leaf residue in collection step (1), the water of 4 times of quality is added, adds the biology of 0.1% mass ratio
(beta-glucosidase, cellulase, the compound proportion of live bacillus subtilis preparation are 1 to preparation：1：1) after, stirring,
Hydrolysed ferment 12h is incubated under the conditions of pH6, temperature 50 C, obtains hydrolysed ferment liquid.Food-grade is added into hydrolysed ferment liquid
95% ethanol (in system alcohol concentration be 40%), in 65 DEG C of water bath with thermostatic control refluxing extractions 2 times, merging filtrate after filtering, obtain
Ginkgo biloba succi B.
(3) after ginkgo biloba succi A and ginkgo biloba succi B is merged, it is concentrated under reduced pressure as the 1/4 of original volume.To system
The middle water for adding 5 times, after stirring, 8h is stood, the 1/4 of original volume is concentrated under reduced pressure into after taking supernatant liquid filtering, be this hair
Bright described ginkgo biloba p.e.
Embodiment three
(1) by fresh ginkgo leaf cleaning drain away the water after, cut into serving pieces, then by sheet ginkgo leaf with 45% ethanol it is molten
Liquid is according to 1:After 6 ratio mixing, it is put into microwave abstracting equipment and is extracted, microwave power is set to 450W, and system temperature is set
To rise to 50~65 DEG C from room temperature in 10~30min, after handling 0.7h, filtering and collecting filter liquid, ginkgo biloba succi is obtained
A。
(2) the ginkgo leaf residue in collection step (1), the water of 3 times of quality is added, adds the biology of 0.3% mass ratio
(beta-glucosidase, cellulase, the compound proportion of live bacillus subtilis preparation are 1 to preparation：2：0.5), stir
Afterwards, hydrolysed ferment 9h is incubated under the conditions of pH5.5,35 DEG C of temperature, obtains hydrolysed ferment liquid.Food is added into hydrolysed ferment liquid
95% ethanol of level (in system alcohol concentration be 35%), in 70 DEG C of water bath with thermostatic control refluxing extractions 3 times, merging filtrate after filtering, obtain
To ginkgo biloba succi B.
(3) after ginkgo biloba succi A and ginkgo biloba succi B is merged, it is concentrated under reduced pressure as the 1/4 of original volume.To system
The middle water for adding 7 times, after stirring, 7h is stood, the 1/3 of original volume is concentrated under reduced pressure into after taking supernatant liquid filtering, be this hair
Bright described ginkgo biloba p.e.
Example IV
(1) by fresh ginkgo leaf cleaning drain away the water after, cut into serving pieces, then by sheet ginkgo leaf with 55% ethanol it is molten
Liquid is according to 1:After 8 ratio mixing, it is put into microwave abstracting equipment and is extracted, microwave power is set to 650W, and system temperature is set
To rise to 50~65 DEG C from room temperature in 10~30min, after handling 0.8h, filtering and collecting filter liquid, ginkgo biloba succi is obtained
A。
(2) the ginkgo leaf residue in collection step (1), the water of 4 times of quality is added, adds the biology of 0.4% mass ratio
(beta-glucosidase, cellulase, the compound proportion of live bacillus subtilis preparation are 1 to preparation：1：0.4), stir
Afterwards, hydrolysed ferment 16h is incubated under the conditions of pH4.5,40 DEG C of temperature, obtains hydrolysed ferment liquid.Food is added into hydrolysed ferment liquid
The ethanol of grade 95% (in system alcohol concentration be 50%), in 75 DEG C of water bath with thermostatic control refluxing extractions 2 times, merging filtrate after filtering,
Obtain ginkgo biloba succi B.
(3) after ginkgo biloba succi A and ginkgo biloba succi B is merged, it is concentrated under reduced pressure as the 1/4 of original volume.To system
The middle water for adding 8 times, after stirring, 10h is stood, the 1/5 of original volume is concentrated under reduced pressure into after taking supernatant liquid filtering, be this hair
Bright described ginkgo biloba p.e.
Application test
During the pipe tobacco of production of cigarettes rolls, ginkgo biloba p.e is added in pipe tobacco, ginkgo biloba p.e accounts for
The 0.2% of pipe tobacco weight.Specific method is that first ginkgo biloba p.e is dissolved in ethanol, and the ethanol solution dissolved with extract is equal
Even to be sprayed on pipe tobacco, subsequent ethanol evaporation is fallen, and pipe tobacco rolls into cigarette sample through high-speed cigarette machine.With being not added with extract
Blank cigarette is evaluated as control with reference to State Bureau's standard of smokeing panel test.Group of smokeing panel test is made up of 13 cigarette sensory evaluation experts,
Using secretly commenting mode to carry out sensory evaluation and giving a mark, Analyses Methods for Sensory Evaluation Results is as shown in table 1.
The cigarette sensory effects evaluation result of table 1
Using fFlue gas collector and electron spin resonance, to cigarette master after analysis detection addition ginkgo biloba p.e
The elimination effect of flue gas free-radical contents is flowed, as a result as shown in table 2.
Elimination effect of the ginkgo biloba p.e of table 2 to cigarette mainstream flue gas free-radical contents
Note：+, free-radical contents rise；-, free-radical contents reduce
After adding ginkgo biloba p.e, the sensory effects evaluation score of cigarette significantly improves, and application effect is more notable, individual
Property note protrude, aromatic flavour is full, pure comfortable, and lasting is lasting, and pleasant impression is totally comfortable, the soft exquisiteness of flue gas, and perfuming
Effect stability；Simultaneously during cigarette is burnt and sucked have remove cigarette mainstream flue gas in free-radical contents the effect of.
The detailed description of specific embodiments of the present invention is the foregoing is only, the present invention is not limited with this, it is all at this
All any modification, equivalent and improvement made in the mentality of designing of invention etc., should be included in protection scope of the present invention it
It is interior.
Claims (6)
1. a kind of preparation method of ginkgo biloba p.e, it is characterised in that comprise the following steps：
(1) cut into serving pieces, it is then 30- by sheet ginkgo leaf and mass concentration after the cleaning of fresh ginkgo leaf is drained away the water
60% ethanol solution is according to 1:After 5-10 mass ratio mixing, it is put into microwave abstracting equipment and is extracted, microwave power
350~700W is set to, system temperature is set in 10~30min rise to 50~65 DEG C from room temperature, after handling 0.5h~1h, filtering
After collect filtrate, obtain ginkgo biloba succi A；
(2) the ginkgo leaf residue in collection step (1), the water of 2-5 times of quality is added, by ginkgo leaf residue 0.1-0.5% matter
Amount ratio adds biological agent, after stirring, hydrolysed ferment 6-18h is incubated under the conditions of pH4-6,30-50 DEG C of temperature, is obtained
Hydrolysed ferment liquid, the ethanol of food-grade 95% is added into hydrolysed ferment liquid, in 60-80 DEG C of water bath with thermostatic control refluxing extraction 1-3 times, mistake
Merging filtrate after filter, obtain ginkgo biloba succi B；
(3) will be concentrated under reduced pressure as the 1/4 of original volume after ginkgo biloba succi A and ginkgo biloba succi B merge, into system plus
Enter the water of 5-8 mass multiples, after stirring, stand 6-12h, take the 1/5-1/ that original volume is concentrated under reduced pressure into after supernatant liquid filtering
3, obtain ginkgo biloba p.e.
A kind of 2. preparation method of ginkgo biloba p.e according to claim 1, it is characterised in that：In the step (2)
Biological agent refer to one or more in beta-glucosidase, cellulase, live bacillus subtilis preparation.
A kind of 3. preparation method of ginkgo biloba p.e according to claim 2, it is characterised in that：In the step (2)
Biological agent for two or more compounding when, beta-glucosidase, cellulase, live bacillus subtilis preparation
Compound proportion is 1-2：1-2：0.2-1.
A kind of 4. preparation method of ginkgo biloba p.e according to claim 2, it is characterised in that：In the step (2)
Beta-glucosidase and the enzyme activity of cellulase be the u/g of 5-20 ten thousand, gemma containing withered grass in live bacillus subtilis preparation
Bacillus viable count is not less than 1.0*108CFU/g。
A kind of 5. preparation method of ginkgo biloba p.e according to claim 1, it is characterised in that：In the step (2)
The amount of the addition ethanol of food-grade 95% makes the mass concentration of ethanol in the solution that water bath with thermostatic control is extracted be 30-60%.
6. the application in cigarette of the ginkgo biloba p.e prepared according to any one of claim 1-5 methods described, its feature
It is：The ginkgo biloba p.e is added in cigarette as natural tobacco additive, and adding proportion is tobacco quality
0.1%-0.3%, concretely comprise the following steps：First ginkgo biloba p.e is dissolved in ethanol, the ethanol solution dissolved with extract is uniform
It is sprayed on pipe tobacco, subsequent ethanol evaporation is fallen, and pipe tobacco rolls into cigarette sample through high-speed cigarette machine.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108264965A (en) * | 2018-04-12 | 2018-07-10 | 江西中烟工业有限责任公司 | A kind of preparation method of mud wormwood artemisia enzymatic hydrolyzed extract and its application in cigarette |
| CN108851194A (en) * | 2018-07-17 | 2018-11-23 | 陈汉林 | A kind of honeysuckle health care cigarette and preparation method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010079406A (en) * | 2001-07-12 | 2001-08-22 | 김진경 | A making method of the cigars or the cigarettes of added with caffeine. |
| CN102031194A (en) * | 2009-09-28 | 2011-04-27 | 福建中烟工业公司 | Ginkgo leaf volatile oil extraction method and method for flavoring cigarettes by utilizing volatile oil |
| CN103060095A (en) * | 2013-01-25 | 2013-04-24 | 安徽中烟工业有限责任公司 | Indocalamus leaf ethanol extract and application of indocalamus leaf ethanol extract in cigarette |
| CN103300469A (en) * | 2013-06-27 | 2013-09-18 | 南京林业大学 | Ginkgo leaf cigarette and preparation method thereof |
| CN105687035A (en) * | 2016-02-04 | 2016-06-22 | 江苏佰康生物科技股份有限公司 | Compound ginkgo essential oil with effects of resisting oxidation and delaying skin aging |
| CN105950297A (en) * | 2016-06-20 | 2016-09-21 | 李颖 | Novel perfumed flavor oil and preparation method thereof |
| CN107118848A (en) * | 2017-07-05 | 2017-09-01 | 湖北中烟工业有限责任公司 | A kind of fine processing method of coconut oil and its application |
| CN107232638A (en) * | 2017-07-05 | 2017-10-10 | 湖北中烟工业有限责任公司 | A kind of upgrading purification process of raisin extract and its application |
-
2017
- 2017-12-06 CN CN201711273705.0A patent/CN107802025B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010079406A (en) * | 2001-07-12 | 2001-08-22 | 김진경 | A making method of the cigars or the cigarettes of added with caffeine. |
| CN102031194A (en) * | 2009-09-28 | 2011-04-27 | 福建中烟工业公司 | Ginkgo leaf volatile oil extraction method and method for flavoring cigarettes by utilizing volatile oil |
| CN103060095A (en) * | 2013-01-25 | 2013-04-24 | 安徽中烟工业有限责任公司 | Indocalamus leaf ethanol extract and application of indocalamus leaf ethanol extract in cigarette |
| CN103300469A (en) * | 2013-06-27 | 2013-09-18 | 南京林业大学 | Ginkgo leaf cigarette and preparation method thereof |
| CN105687035A (en) * | 2016-02-04 | 2016-06-22 | 江苏佰康生物科技股份有限公司 | Compound ginkgo essential oil with effects of resisting oxidation and delaying skin aging |
| CN105950297A (en) * | 2016-06-20 | 2016-09-21 | 李颖 | Novel perfumed flavor oil and preparation method thereof |
| CN107118848A (en) * | 2017-07-05 | 2017-09-01 | 湖北中烟工业有限责任公司 | A kind of fine processing method of coconut oil and its application |
| CN107232638A (en) * | 2017-07-05 | 2017-10-10 | 湖北中烟工业有限责任公司 | A kind of upgrading purification process of raisin extract and its application |
Non-Patent Citations (2)
| Title |
|---|
| 丁兴红等: "纤维素酶辅助提取银杏叶总黄酮的工艺条件", 《林业科技开发》 * |
| 刘峙嵘等: "微波萃取银杏叶黄酮类化合物", 《东华理工学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108264965A (en) * | 2018-04-12 | 2018-07-10 | 江西中烟工业有限责任公司 | A kind of preparation method of mud wormwood artemisia enzymatic hydrolyzed extract and its application in cigarette |
| CN108264965B (en) * | 2018-04-12 | 2021-06-08 | 江西中烟工业有限责任公司 | Preparation method of artemisia scoparia enzymolysis extract and application of artemisia scoparia enzymolysis extract in cigarettes |
| CN108851194A (en) * | 2018-07-17 | 2018-11-23 | 陈汉林 | A kind of honeysuckle health care cigarette and preparation method thereof |
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|---|---|
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