CN107792837A - The nanometer selenium for preparing the method for nanometer selenium using camellia plant nanometer aggregation and being prepared - Google Patents
The nanometer selenium for preparing the method for nanometer selenium using camellia plant nanometer aggregation and being prepared Download PDFInfo
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Abstract
The invention belongs to technical field of nano material, discloses a kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.This method comprises the following steps：(1) separation of camellia plant nanometer aggregation：Camellia plant water extract is prepared, camellia plant nanometer aggregation, average diameter ＞ 10nm and the class micelles of average diameter≤10nm two are centrifuged by ultrafiltration；(2) nanometer selenium is prepared using camellia plant nanometer aggregation：Sodium selenite occurs reduction reaction with vitamin C under the conditions of existing for camellia plant nanometer aggregation and obtains nano selenium sol, and removing vitamin C obtains nanometer selenium finished product.The present invention discloses the difference of different camellia plant nanometer aggregation nanometer seleniums using camellia plant nanometer aggregation as template, strengthens nanometer selenium functional characteristic, widens the application field of nanometer selenium, improve its application value.
Description
Technical field
It is more particularly to a kind of to prepare nanometer using camellia plant nanometer aggregation the invention belongs to technical field of nano material
The method of selenium and the nanometer selenium being prepared.
Background technology
Selenium (Se) is one of micro elements needed by human, closely related with health.Anti-oxidant, anticancer is that selenium is main
Bioactivity.Selenium also has simultaneously improves immunity, antagonism heavy metal, participates in internal iodine metabolism, adjusts iodine in organism
Horizontal and Free Radical Level etc..Selenium is a double-edged sword, and toxicity is also produced simultaneously playing anti-oxidant, active anticancer.The life of selenium
Thing effect and the toxic concentration scope of selenium are extremely narrow, and toxicity is just shown just over nutrition dosage.Research finds, selenium and selenium
Compound toxicity size is：Inorganic selenium>Organic Selenium>Elemental selenium.
In nature, elemental selenium mainly exists in the form of three kinds：Gray selenium, black selenium and red elemental selenium.Red elemental selenium chi
Very little scope is nanoscale, therefore referred to as nanometer selenium.Research shows：Compared to inorganic selenium and Organic Selenium, nanometer selenium has high bioactivity
With hypotoxicity, but it instability limit its application, therefore the preparation method for finding high-stability nano selenium turns into and ground
The main flow studied carefully.
The preparation method of nanometer selenium includes：Polymer template method, solid reaction process, microemulsion method, sonochemical method and
Temperature control method etc..For polymer template method because it can assign template function to nanometer selenium, reaction condition is gentle, simple to operate,
Particle size is controllable to turn into the common method for preparing nanometer selenium.Therefore, the selection of template is of crucial importance.
Currently used template is mainly functional active components, surfactant etc., including：Functional polysaccharide, protein
And polypeptide etc..
The content of the invention
In order to overcome shortcoming and defect present in prior art, primary and foremost purpose of the invention is that providing one kind utilizes mountain
The method that tea plant Micelle-like Nano-structure of Two prepares nanometer selenium；This method using the Micelle-like Nano-structure of Two of different camellia plant origins as template,
The different nanometer selenium of particle diameter is prepared, its particle diameter is proportionate with template particle size；In addition, different camellia plant nanometer aggregations
Body activity constituent and content are different, cause its functional activity to differ, and prepare function intensified camellia plant nanometer aggregation
Nanometer selenium, gained camellia plant nanometer aggregation nanometer selenium can be applied to different health care fields.
The nanometer selenium being prepared another object of the present invention is to provide a kind of above method.
The purpose of the present invention is achieved through the following technical solutions：
A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation, is comprised the following steps：
(1) camellia plant nanometer aggregation is separated；
(2) nanometer selenium is prepared using camellia plant nanometer aggregation.
Step (1) the separation camellia plant nanometer aggregation is specifically according to following steps：By camellia plant material by material
Liquor ratio 1:10~1:250 add 25~100 DEG C of water, and 2~120min is then extracted under 25~100 DEG C of water bath conditions obtains camellia
Plant extraction liquid, reuse molecular cut off be 3~100kDa centrifugal ultrafiltration pipe in 4 DEG C of temperature, 1000~4000g of centrifugal force
Under the conditions of centrifuge 10~40min, collect inner and outer tubes obtain the Micelle-like Nano-structure of Two solution of different-grain diameter scope, be freeze-dried,
In -20 DEG C of preservations, camellia plant nanometer aggregation is obtained.
The camellia plant material is Theaceae (Theaceae) plant material, including tealeaves (Camellia
) or Cocoa (Camellia ptilophylla) sinensis.
More preferred, the solid-liquid ratio is 1:50；The temperature of the water-bath is 100 DEG C；The time of the extraction is
30min；The centrifugal ultrafiltration pipe molecular cut off is 100kD；The centrifugal force is 4000g；The time of the centrifugation is
20min；Gained camellia plant nanometer aggregation is average diameter ＞ 10nm and the class micelles of average diameter≤10nm two.
Step (2) is described to prepare nanometer selenium specifically according to following steps using camellia plant nanometer aggregation：
A, it is 1 by mol ratio:2~1:20 sodium selenite solution is with vitamin c solution in camellia plant nanometer aggregation
Reduction reaction occurs under the conditions of existing, stands 0.5~72h in 20~80 DEG C of water-baths, obtains nano selenium sol；The selenous acid
The concentration of sodium solution is 10~100mM, and ascorbic concentration is 100~500mM；The camellia plant nanometer aggregation adds
Dosage is 25~900mg/L；
B, nano selenium sol through being centrifuged at a high speed, ultrafiltration centrifuge or dialysis separation, remove vitamin C, received
Rice selenium suspension, freeze-drying obtain solid nano selenium；It is described be centrifuged at a high speed be under the conditions of 4 DEG C, with rotating speed 8000~
11000r/min centrifuges 20~40min, and it is to use 3~100kDa centrifugal ultrafiltration pipes that the ultrafiltration, which centrifuges, under the conditions of 4 DEG C
10~40min is centrifuged with 1000~4000g of rotating speed, the dialysis separation is to use 3.5~8000kDa regenerated cellulose bag filters
Dialyse 12~96h.
The concentration of sodium selenite solution described in step A is 10mM, and ascorbic concentration is 100mM；The sodium selenite is molten
The mol ratio of liquid and vitamin c solution is 1:8；The addition of the camellia plant nanometer aggregation is 25mg/L or 50mg/L；
The temperature of the water-bath is 25 DEG C, and the time of standing is 24h.
A kind of nanometer selenium being prepared according to above-mentioned method.
Tumor cell survival is detected by mtt assay, evaluates the active anticancer of present invention gained nanometer selenium；It is anti-by chemistry
Scorching method and inflammatory cell model, evaluate the anti-inflammatory activity of present invention gained nanometer selenium.
Above-mentioned tumor models include：The cell lines of HCT 116, Hepa1c1c7 cell lines, MDA-MB-231 cell lines,
HepG2 cell lines, Hela cell lines etc., the preferably cell lines of HCT 116.
Above-mentioned chemical anti-inflammatory method is with reference to the methods of Tsai.
Above-mentioned inflammatory cell model includes：Raw264.7 cell lines, Caco-2 cell lines, HUVEC cell lines etc., preferably
Raw264.7 cell lines.
Above-mentioned anti-inflammatory activity measure includes：Chemical anti-inflammatory method and cell method, preferred cell method.
The present invention is had the following advantages relative to prior art and beneficial effect：
The present invention is using camellia plant nanometer aggregation as template；In camellia plant containing a large amount of Polyphenols, soaping agents,
The multiple functions active component such as alkaloid, there is the health efficacies such as anti-oxidant, anti-mutation, anticancer, anti-inflammatory, reducing blood lipid；Nanometer is poly-
When collective is that compound reaches certain critical concentration in the solution, the intermolecular nanoscale formed by certain active force aggregation
Micelle, it is intermediateness of the compound between free molecule and precipitation particle, is the base unit of deposition activity composition, is different
Compound plays one of important results basis of Synergistic/antagonistic effect；With Theaceae (Camellia sinensis) tealeaves
Exemplified by Micelle-like Nano-structure of Two, tealeaves Micelle-like Nano-structure of Two is based on protein and Tea Polyphenols and its oxide；Tea Polyphenols and its oxidation
Thing contains great amount of hydroxy group and water repellent region, and protein can pass through electrostatic force or thin containing amino, carboxyl and carbonyl etc., each group
Aqueous phase interaction is combined with nanometer selenium, fully dispersed stable nanometer selenium；In addition, Tea Polyphenols etc. is the material base of tea health-care effect
Plinth, nanometer selenium is prepared by template of tealeaves Micelle-like Nano-structure of Two, nano selenium tea effect will be assigned, obtain its anti-oxidant, active anticancer
With Synergistic.
Brief description of the drawings
Fig. 1 is differential responses thing proportioning and influence of the reaction time to nanometer selenium particle diameter.
Fig. 2 is influence of the different templates addition to nanometer selenium particle diameter.
Fig. 3 is influence of the different method for separating and processing to nanometer selenium particle diameter.
Fig. 4 is influence of the differential responses thing concentration to nanometer selenium particle diameter.
Fig. 5 is templating nanoparticles selenium to process the Micelle-like Nano-structure of Two (average diameter ＞ 10nm) of dry tea with different primary making process
Particle diameter.
Fig. 6 is templating nanoparticles selenium to process the Micelle-like Nano-structure of Two (average diameter≤10nm) of dry tea with different primary making process
Particle diameter.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
In order that the purpose of the present invention, technical scheme become apparent from understanding, with reference to tea (Camellia
Sinensis), Cocoa (Camellia ptilophylla) fresh leaf processes the Micelle-like Nano-structure of Two of dry tea through different primary making process
Illustrated as template, the present invention will be described in further detail.Device therefor instrument and reagent of the present invention are this area institute
It is conventional.It should be appreciated that citing described herein is not intended to limit the present invention only to explain the present invention.
Embodiment 1：A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.
(1) 4.5g green tea accurately is weighed in 250mL conical flasks, add boiling water 225mL, boiling water bath 30min, per 10min
Vibration once, filters while hot, cools down constant volume.
(2) 4mL millet paste is pipetted in 100KDa super filter tube inner tubes, in 4 DEG C, 4000g, centrifuges 20min；Collect inside/outside Guan Rong
Liquid obtains different-grain diameter scope (DH＞ 10nm and DH≤ 10nm) Micelle-like Nano-structure of Two solution, be freeze-dried to obtain freeze-dried powder, it is stand-by.
(3) 10mM sodium selenite solution, 100mM vitamin c solution is prepared respectively.
(4) green tea Micelle-like Nano-structure of Two freeze-dried powder (D is weighed respectivelyH＞ 10nm) 40mg (400mg/L) in 5mL centrifuge tubes,
It is transferred to 10mL ultra-pure waters in beaker, adds 72mL ultra-pure waters, stir, crosses 0.8 μm of filter membrane.Fixed system sodium selenite
Concentration is 1mM, is matched respectively according to differential responses thing, Vc:Sodium selenite 2:1、3:1、4:1、5:1、6:1、8:1、10:1、20:1
(mmol:Mmol a certain amount of 10mM sodium selenite solution) is added, 15min is stood after well mixed, then adds a certain amount of
100mM vitamin c solution, mix, reaction stands 24h, obtains nanometer selenium colloidal solution；
(5) by above-mentioned nanometer selenium colloidal solution, 24h is dialysed by 3.5kDa regenerated celluloses bag filter to remove unreacted
Vitamin C, nanometer selenium colloidal solution properties of colloid chemistry is determined, as a result as shown in figure 1, reactant ratio is 8:When 1, nanometer selenium
Average diameter is smaller, and numbers of particles is more, and system is relatively stable.
(6) the nanometer selenium colloidal solution after dialysing is freeze-dried to obtain solid nano selenium powder, stand-by in -20 DEG C of preservations.
Embodiment 2：A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.
Compared with Example 1, distinctive points are only that：
Green tea Micelle-like Nano-structure of Two freeze-dried powder (D is weighed respectivelyH＞ 10nm) 2.5,5,10,20,30,40,50,50mg (25,
50th, 100,200,300,400,500,600mg/L) in 5mL centrifuge tubes, it is transferred in beaker, is added with 10mL ultra-pure waters
72mL ultra-pure waters, stir, and cross 0.8 μm of filter membrane.Fixed system concentration of sodium selenite is 1mM, according to reactant ratio, Vc:
Sodium selenite 8:1(mmol：Mmol a certain amount of 10mM sodium selenite solution) is added, 15min, Ran Houjia are stood after well mixed
Enter a certain amount of 100mM vitamin c solution, mix, reaction stands 24h, obtains nanometer selenium colloidal solution, determines its colloid chemistry
Characteristic, as a result as shown in Fig. 2 considering, when template concentrations are 25mg/L and 50mg/L, nanometer selenium average diameter is smaller, receives
Rice selenium suspension is relatively stable without coagulation, system.
Embodiment 3：A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.
Compared with Example 1, distinctive points are only that：
Green tea Micelle-like Nano-structure of Two freeze-dried powder (D is weighed respectivelyH＞ 10nm) 40mg (400mg/L) is in 5mL centrifuge tubes, use
10mL ultra-pure waters are transferred in beaker, are added 72mL ultra-pure waters, are stirred, and cross 0.8 μm of filter membrane.Fixed system sodium selenite is dense
Spend for 1mM, according to reactant ratio, Vc:Sodium selenite 8:1(mmol:Mmol a certain amount of 10mM sodium selenite solution) is added,
Stand 15min after well mixed, then add a certain amount of 100mM vitamin c solution, mix, reaction stands 4,6,8,12,
24th, 36,48,72h, obtains nanometer selenium colloidal solution, determines its properties of colloid chemistry, as a result as shown in figure 1, the reaction time is
During 24h, nanometer selenium average diameter is smaller, and system total light intensity is larger, and system is relatively stable.
Embodiment 4：A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.
Compared with Example 1, distinctive points are only that：
Green tea Micelle-like Nano-structure of Two freeze-dried powder (D is weighed respectivelyH＞ 10nm) 2.5mg (25mg/L) is in 5mL centrifuge tubes, use
10mL ultra-pure waters are transferred in beaker, are added 72mL ultra-pure waters, are stirred, and cross 0.8 μm of filter membrane.Fixed system sodium selenite is dense
Spend for 1mM, according to reactant ratio, Vc:Sodium selenite 8:1(mmol：Mmol a certain amount of 10mM sodium selenite solution) is added,
15min is stood after well mixed, then adds a certain amount of 100mM vitamin c solution, is mixed, reaction stands 24h, is received
Rice selenium colloidal solution；
By above-mentioned nanometer selenium colloidal solution, high speed centrifugation 40min, 4 DEG C, 4000g are freezed by 4 DEG C, 11000rpm/min
Ultrafiltration centrifuges 20min or 3.5kDa regenerated celluloses bag filter dialysis 24h to remove unreacted vitamin C, determines nanometer
Selenium colloidal solution properties of colloid chemistry, as a result as shown in figure 3, dialysis separation influences minimum to nanometer selenium average diameter, before dialysis
Average diameter is without significant difference afterwards.
Embodiment 5：A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.
(1) 100mM sodium selenite solution, 500mM vitamin c solution is prepared respectively.
(2) green tea Micelle-like Nano-structure of Two freeze-dried powder (D is weighed respectivelyH＞ 10nm) 2.5,12.5,25,37.5,50mg is (with reaction
Thing concentration equimultiple amplifies template addition：5 times, 10 times, 15 times, 20 times) in 5mL centrifuge tubes, it is transferred to 10mL ultra-pure waters
In beaker, 72mL ultra-pure waters are added, are stirred, cross 0.8 μm of filter membrane.Respectively according to reactant ratio, Vc:Sodium selenite 8:1、
40:5、80:10、120:15、160:20(mmol:Mmol a certain amount of 100mM sodium selenite solution) is added, it is quiet after being well mixed
15min is put, then adds a certain amount of 500mM vitamin c solution, is mixed, reaction stands 24h, obtains nanometer selenium colloidal solution；
(3) by above-mentioned nanometer selenium colloidal solution, 24h is dialysed by 3.5kDa regenerated celluloses bag filter to remove unreacted
Vitamin C, determine nanometer selenium colloidal solution properties of colloid chemistry, as a result as shown in figure 4, being improved with reactant concentration, nanometer selenium
Average diameter reduces, and nano granules of selenium number increases, but system stability reduces, therefore reactant ratio is preferably 8:1.
(4) the nanometer selenium colloidal solution after dialysing is freeze-dried to obtain solid nano selenium powder, stand-by in -20 DEG C of preservations.
Embodiment 6：A kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation.
Compared with Example 1, distinctive points are only that：
Accurately weigh respectively 4.5g green tea, white tea, yellow tea, oolong tea, black tea, Pu'er tea, cocoa green tea, cocoa black tea in
In 250mL conical flasks, boiling water 225mL, boiling water bath 30min are added, vibration once, filters while hot per 10min, cools down constant volume.
Green tea, white tea, yellow tea, oolong tea, black tea, Pu'er tea, Cocoa green tea, Cocoa black tea nanometer is weighed respectively to gather
Collective (DH＞ 10nm and DH≤ 10nm) in 5mL centrifuge tubes, beaker is transferred to 10mL ultra-pure waters freeze-dried powder 2.5mg and 5mg
In, 72mL ultra-pure waters are added, are stirred, cross 0.8 μm of filter membrane, add 10mL 10mM sodium selenite solution, after being well mixed
15min is stood, then adds 8mL 100mM vitamin c solution, is mixed, reaction stands 24h, obtains nanometer selenium colloidal solution,
For its properties of colloid chemistry result as shown in Figure 5 and shown in Fig. 6, template adds concentration when being 25mg/L, nanometer selenium average diameter compared with
It is small；Same template concentrations, using average diameter≤10nm millet paste Micelle-like Nano-structure of Two as nanometer selenium average diameter prepared by template compared with
It is small.Nanometer selenium average diameter size is related to template particle size.
Embodiment 7：The active anticancer measure of nanometer selenium
Single cell suspension is made by the adherent cells of HCT 116 of Trypsin Induced, with 5 × 104Individual cells/well inoculation 96
Well culture plate, it is placed in 37 DEG C, 5%CO2Preculture 24h in incubator, the millet paste nanometer aggregation of different primary making process processing dry teas
Body (DH＞ 10nm) for template (template amount is respectively 25mg and 50mg) nanometer selenium dissolved with culture medium after, add by 100 μ L/ holes
Enter culture medium, continue to cultivate 72h.Add the μ L/ holes of 0.05mg/mL MTT dilutions 100 toward culture plate, be placed on 37 DEG C, 5%
CO2Continue to abandon supernatant after cultivating 2h in incubator, 200 μ L DMSO solutions are added per hole, are placed in 100rpm/min shaking table
15~20min is vibrated, determines each hole OD550Value, with control group OD550It is worth for 100%, calculates each group nanometer selenium and suppress cell propagation
503nhibiting concentration (IC50), as shown in table 1, when template addition is 25mg/L or 50mg/L, with green tea, white tea, yellow tea, crow
Imperial tea, Pu'er tea, Cocoa green tea DH＞ 10nm Micelle-like Nano-structure of Two is that nanometer selenium prepared by template is respectively provided with stronger anti-increasing
Activity is grown, wherein especially most strong as the nanometer selenium active anticancer of template using green tea Micelle-like Nano-structure of Two.
Table 1 processes the Micelle-like Nano-structure of Two (D of dry tea with different primary making processH＞ 10nm) for template nanometer selenium suppress HCT
503nhibiting concentration (the IC of 116 cells propagation50)
(sample concentration gradient is 1.5625,3.125,6.25,12.5,25,50 μ g/mL；Received with black tea, Cocoa black tea
Rice aggregation for template nanometer selenium in this concentration range, cell survival rate is all higher than 0.5.)
Embodiment 8：The anti-inflammatory activity measure of nanometer selenium
Adherent Raw264.7 cells are scraped by scraper, piping and druming is dispersed into single cell suspension, with 5 × 105Individual cells/well
96 well culture plates are inoculated with, are placed in 37 DEG C, 5%CO2Preculture 24h in incubator, the millet paste of different primary making process processing dry teas are received
Rice aggregation (DH≤ 10nm) be template (template amount is 25mg) nanometer selenium dissolved with culture medium after, by 100 μ L/ holes add train
Base is supported, and adds the μ L/ holes of 1 μ g/mL LPS working solutions 100, continues to cultivate 24h.Sample culturing base 100 in culture plate is taken out respectively
μ L/ holes add the μ L/ holes of Griess reagents 100, stand 10min, determine OD in new culture plate542Value, calculate each group nanometer selenium
To inhibiting rate caused by NO.After old culture plate abandons nutrient solution, the μ L/ holes of 0.05mg/mL MTT dilutions 100 are added, are placed on 37
DEG C, 5%CO2Continue to abandon supernatant after cultivating 1h in incubator, 200 μ L DMSO solutions are added per hole, are placed on 100rpm/min's
10~15min is vibrated in shaking table, determines each hole OD550Value, with control group OD550It is worth for 100%, calculates cell survival rate.With thin
Born of the same parents' survival rate>50%, calculate each group nanometer selenium and suppress 503nhibiting concentration (IC caused by NO50), as shown in table 2, with green tea, in vain
Micelle-like Nano-structure of Two (the D of tea, oolong tea and Cocoa green tea, black teaH≤ 10nm) for template prepare nanometer selenium have it is stronger
Anti-inflammatory activity.
Table 2 processes the Micelle-like Nano-structure of Two (D of dry tea with different primary making processH≤ 10nm) be template nanometer selenium pair
503nhibiting concentration (IC caused by Raw264.7 cells NO50)
(sample concentration gradient is 3.125,6.25,12.5,25,50,100 μ g/mL；With yellow tea, black tea, Pu'er tea nanometer
Aggregation for template nanometer selenium in this concentration range, its NO inhibiting rate is below 0.5.)
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (7)
- A kind of 1. method that nanometer selenium is prepared using camellia plant nanometer aggregation, it is characterised in that comprise the following steps：(1) camellia plant nanometer aggregation is separated；(2) nanometer selenium is prepared using camellia plant nanometer aggregation.
- 2. a kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation according to claim 1, its feature are existed In：Step (1) the separation camellia plant nanometer aggregation is specifically according to following steps：Camellia plant material is pressed into solid-liquid ratio 1: 10~1:250 add 25~100 DEG C of water, and 2~120min is then extracted under 25~100 DEG C of water bath conditions obtains camellia plant and carry Take liquid, reuse molecular cut off be 3~100kDa centrifugal ultrafiltration pipe under the conditions of 4 DEG C of temperature, 1000~4000g of centrifugal force 10~40min is centrifuged, inner and outer tubes is collected and obtains the Micelle-like Nano-structure of Two solution of different-grain diameter scope, be freeze-dried, in -20 DEG C Preserve, obtain camellia plant nanometer aggregation.
- 3. a kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation according to claim 2, its feature are existed In：The camellia plant material is plant of theaceae raw material, including tealeaves or Cocoa.
- 4. a kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation according to claim 2, its feature are existed In：The solid-liquid ratio is 1:50；The temperature of the water-bath is 100 DEG C；The time of the extraction is 30min；The centrifugal ultrafiltration Pipe molecular cut off is 100kD；The centrifugal force is 4000g；The time of the centrifugation is 20min；Gained camellia plant nanometer Aggregation is average diameter ＞ 10nm and the class micelles of average diameter≤10nm two.
- 5. a kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation according to claim 1, its feature are existed In：Step (2) is described to prepare nanometer selenium specifically according to following steps using camellia plant nanometer aggregation：A, it is 1 by mol ratio:2~1:20 sodium selenite solution exists with vitamin c solution in camellia plant nanometer aggregation Under conditions of reduction reaction occurs, stand 0.5~72h in 20~80 DEG C of water-baths, obtain nano selenium sol；The sodium selenite is molten The concentration of liquid is 10~100mM, and ascorbic concentration is 100~500mM；The addition of the camellia plant nanometer aggregation For 25~900mg/L；B, nano selenium sol through being centrifuged at a high speed, ultrafiltration centrifuge or dialysis separation, remove vitamin C, obtain nanometer selenium Suspension, freeze-drying obtain solid nano selenium；Described be centrifuged at a high speed is under the conditions of 4 DEG C, with 8000~11000r/ of rotating speed Min centrifuges 20~40min, and it is to use 3~100kDa centrifugal ultrafiltration pipes that the ultrafiltration, which centrifuges, with rotating speed under the conditions of 4 DEG C 1000~4000g centrifuges 10-40min, and the dialysis separation is using 3.5~8000kDa regenerated celluloses bag filter dialysis 12 ~96h.
- 6. a kind of method that nanometer selenium is prepared using camellia plant nanometer aggregation according to claim 5, its feature are existed In：The concentration of sodium selenite solution described in step A is 10mM, and ascorbic concentration is 100mM；The sodium selenite solution with The mol ratio of vitamin c solution is 1:8；The addition of the camellia plant nanometer aggregation is 25mg/L or 50mg/L；It is described The temperature of water-bath is 25 DEG C, and the time of standing is 24h.
- A kind of 7. nanometer selenium that method according to claim any one of 1-6 is prepared.
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| CN109806782A (en) * | 2019-01-31 | 2019-05-28 | 华南农业大学 | A kind of Pickering lotion and its preparation method and application stable based on dark green tea Micelle-like Nano-structure of Two |
| CN109864931A (en) * | 2019-01-31 | 2019-06-11 | 华南农业大学 | A kind of Pickering lotion and its preparation method and application stable using theabrownin |
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| CN113772635A (en) * | 2021-09-16 | 2021-12-10 | 华南农业大学 | Method for preparing nano-selenium by compounding polyphenol compounds and protein and prepared nano-selenium |
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