CN107789207A - A kind of EGF/ vitamin e acetates cyclodextrin liposome and preparation method thereof, application method, application - Google Patents
A kind of EGF/ vitamin e acetates cyclodextrin liposome and preparation method thereof, application method, application Download PDFInfo
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- CN107789207A CN107789207A CN201711068465.0A CN201711068465A CN107789207A CN 107789207 A CN107789207 A CN 107789207A CN 201711068465 A CN201711068465 A CN 201711068465A CN 107789207 A CN107789207 A CN 107789207A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/738—Cyclodextrins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The present invention relates to a kind of EGF/ vitamin e acetates cyclodextrin liposome.Its raw material forms in parts by weight, including 30 100 parts of 2 50 parts of EGF stostes, 2 50 parts of vitamin e acetate, cyclodextrin or cyclodextrine derivatives, 100 220 parts of phospholipid material, 20 70 parts of cholesterol, 40 155 parts of freeze drying protectant.The present invention also provides a kind of preparation method of EGF/ vitamin e acetates cyclodextrin liposome.The present invention also provides a kind of application method of EGF/ vitamin e acetates cyclodextrin liposome.The present invention also provides a kind of application of EGF/ vitamin e acetates cyclodextrin liposome in cosmetics.Beneficial effect：EGF, Vitwas E are rolled into liposome by the present invention, improve both stability and bioavilability；EGF and Vitwas E can act synergistically simultaneously, realize anti-aging and repair to skin together, reach more preferable skin care result.
Description
Technical field
The present invention relates to a kind of EGF/ vitamin e acetates-cyclodextrin liposome, a kind of EGF/ vitamin Es second is further related to
The preparation method of acid esters-cyclodextrin liposome, further relate to a kind of user of EGF/ vitamin e acetates-cyclodextrin liposome
Method and application.
Background technology
EGF (Epidermal Growth Factoy) Chinese is EGF, is made up of 53 amino acid
(and including the polypeptide of 3 disulfide bond).Montalcini and Cohen professors are because having found EGF and analyzing its structure
And the mechanism of action, Nobel's physiology in 1986 and medical science are won a prize.EGF can stimulate the tyrosine of EGFR
Phosphorylation, reach the effect of repairing hyperplasia, so as to replace aging and dead cell with the cell of new life, fundamentally reach skin
The quick reparation of skin damage, reparation and the anti-wrinkle of wrinkle, sensitive nursing, all kinds of scars dispel reparation and other effects, make
Skin smoothness high resilience, keep skin natural health.
Vitwas E is the stable derivative of vitamin E.Vitwas E can decompose in the presence of enzyme
For alpha-tocopherol, and antioxidation activity is produced, protect the skin from free radical injury.External application can reduce light aging and cause
Around and freckle, remove free radical, but also can protect human body from ultraviolet illumination bands come injury, play anti-aging
Effect.With research deeply, it is of increasing concern in terms of anti-oxidant, anti-aging, removing skin free radical.
In view of EGF and Vitwas E are respectively provided with so good cosmetic result, if can combine both materials
Come, be successfully applied to cosmetics, skin care field, then the anti-aging and beauty that can effectively improve company's cosmetic skin-protection product are repaiied
Multiple effect, meets that consumers in general are higher and higher to cosmetic skin care product and uses quality requirements.
But EGF vitro stabilities are poor, Half-life in vivo is short, and easy in inactivation, bioavilability are low；Vitwas E is
Liposoluble active thing, water-soluble extreme difference, meet light and be oxidized easily, so, both of which is difficult to be directly applied to cosmetics performance
Anti-aging ability, seriously constrain promoting the use of a large area in every field.
Therefore, it is necessary to propose effective technical scheme, solve the above problems.
The content of the invention
The present invention is directed to above-mentioned the deficiencies in the prior art, there is provided a kind of drugloading rate is high, stability is good, sustained-release and controlled release effect
It is good, and to EGF/ vitamin e acetates-cyclodextrin liposome of the functions such as skin anti-aging, reparation.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of EGF/ vitamin e acetates-cyclodextrin liposome, its raw material forms in parts by weight, including EGF stostes
2-50 parts, vitamin e acetate 2-50 parts, cyclodextrin or cyclodextrine derivatives 30-100 parts, phospholipid material 100-220 parts, courage
Sterol 20-70 parts, freeze drying protectant 40-155 parts.
Preferably, the cyclodextrin is beta-schardinger dextrin or hydroxypropyl-β-cyclodextrin.
Preferably, the phospholipid material is selected from soybean lecithin, egg yolk lecithin, hydrogenation yolk phospholipid, hydrogenated soybean phosphorus
Fat, two palm phosphatidyl glycerols, distearyl phosphatidyl glycerol, dilauroyl lecithin, two mustard acyl group lecithin, two nutmegs
At least one of phosphatidyl glycerol, DPPC and DSPC.
Preferably, the freeze drying protectant is in trehalose, mannitol, sorbierite, raffinose, dextran and lactose
It is at least one.
The present invention also provides a kind of preparation method of EGF/ vitamin e acetates-cyclodextrin liposome, and step is as follows：
(1) cyclodextrin or cyclodextrine derivatives be added to the water by proportioning, stirring and dissolving, obtain mass concentration for 5~
60% cyclodextrin or cyclodextrine derivatives solution；
(2) cyclodextrin obtained to step (1) is slowly added dropwise in vitamin e acetate by proportioning or cyclodextrine derivatives is molten
In liquid, strong stirring 1-6h at a temperature of 20-60 DEG C, inclusion compound dispersion liquid is obtained；
(3) the inclusion compound dispersion liquid that step (2) obtains is centrifuged into 60min under centrifugal force 70000g, by supernatant after standing
Liquid is concentrated in vacuo, then by concentrate in 40 DEG C of drys 3-6h of vacuum drying chamber, is obtained powdered vitamin e acetate-ring and pasted
Inclusion compounds；
(4) by proportioning using vitamin e acetate-cyclodextrin inclusion compound that EGF stostes and step (3) obtain as core,
Coated again by proportioning addition phospholipid material, cholesterol, obtain EGF/ vitamin e acetates-cyclodextrin liposome turbid liquor；
(5) the EGF/ vitamin e acetates-cyclodextrin liposome turbid liquor obtained step (4) is freeze-dried, obtains
EGF/ vitamin e acetates-cyclodextrin liposome of the present invention.
Preferably, the step (4) is specially：It is by matching that EGF stostes and vitamin e acetate cyclodextrin inclusion compound is molten
In 0.02M pH7.4 phosphate buffer solution, solution A is obtained；Phospholipid material is mixed with cholesterol and is dissolved in ether,
Obtain solution B；Solution A is added dropwise in solution B, is ultrasonically treated under the conditions of ice-water bath, then is removed under reduced pressure in Rotary Evaporators
Solvent, after solvent is evaporated completely, stops the rotation and keeps vacuum to continue to stop after aspirating 30min, then adds 0.02M's
PH7.4 phosphate buffer solution, the gel being hydrated in bottle wall come off, and using high pressure homogenizer homogeneous 4-5 times, obtain EGF/
Vitamin e acetate-cyclodextrin liposome turbid liquor.
Preferably, the lyophilisation condition of the step (5) is：Carried out using quick-frozen mode, pre-freezing temperature -60 ± 2 DEG C, pre-freeze
Time 6h, condenser temperature -50~-55 DEG C, vacuum ＜ 100mT, freeze-drying time 24h.
The present invention also provides a kind of application method of EGF/ vitamin e acetates-cyclodextrin liposome, is stated using above-mentioned
The preparation method of EGF/ vitamin e acetates-cyclodextrin liposome is prepared；EGF/ vitamin e acetates-the cyclodextrin
The application method of liposome, in use, plus deionized water aquation rebuild, gently concussion dissolving is complete, obtains after rehydration rebuilds
Inclusion liposome suspension, use.
Further, EGF/ vitamin e acetates-cyclodextrin in the inclusion liposome suspension after the rehydration is rebuild
The particle diameter of liposome is 90-200nm.Particle diameter is small after rehydration is rebuild, and preparation method is simple, and envelop rate is high, survivable skin care item
Stable system, it is can be applied to a variety of State of cosmetics such as emulsion, creams, gel, Essence.
The present invention also provides a kind of EGF/ vitamin e acetates-application of the cyclodextrin liposome in cosmetics.This is sent out
Bright EGF/ vitamin e acetates-cyclodextrin liposome, which is added in cosmetics, is made emulsion, eye cream, nourishing cream, massage cream,
Facial gel, eye essence, eye gel, cleansing cream, mildy wash, foundation cream, body lotion, body frost, body gels etc..
Beneficial effect：
(1) EGF, Vitwas E are rolled into liposome by the present invention, improve both stability and biological utilisation
Degree；EGF and Vitwas E can act synergistically simultaneously, realize anti-aging and repair to skin together, reach more preferable
Skin care result.
(2) cyclodextrin material, phospholipid material and the freeze drying protectant in the present invention are the cosmetics original to skin beneficiating
Material, can act synergistically on skin.
(3) because the cuticula phospholipid layer content of human body skin is less, Phospholipids content highest in basalis, therefore this hair
Bright EGF/ vitamin e acetates-cyclodextrin liposome is easier to be dissolved and slowly discharged by Phospholipids in basalis, allows
EGF and vitamin e acetate are preferably absorbed by skin.
(4) EGF/ vitamin e acetates-cyclodextrin liposome of the invention is powdered to be easy to pack, store and transport
It is defeated, using more convenient.
Embodiment
The present invention relates to a kind of EGF/ vitamin e acetates-cyclodextrin liposome, and it is poor, easy to solve EGF vitro stabilities
The shortcomings of inactivation, solves vitamin e acetate poorly water-soluble simultaneously, meets the problem of light is oxidized easily.Its raw material includes EGF
Stoste, vitamin e acetate, cyclodextrin or cyclodextrine derivatives, phospholipid material, cholesterol, freeze drying protectant.Liposome be by
The completely enclosed single or multiple lift vesica for the bilayer that the lipoids such as phosphatide are formed.Its structure is similar to cell, can encapsulate water
Dissolubility and liposoluble substance.Liposome is similar to keratoderma intercellular lipid structure as topical remedy's carrier, can be quickly
Ground enters deep skin through cuticula, more active materials is stayed in epidermis between corium.The stability of liposome obtains
After improvement, it is used as the transmission system of cosmetic active components, increases effect, storage and the protection component of cosmetics；During use
The effect of controllable release (sustained release) lipid composition.
The molecule mol ratio of the vitamin e acetate and cyclodextrin or cyclodextrine derivatives is 1: 2~50.
Cyclodextrin of the present invention is that one kind is joined end to end and formed by more than 6 (being often 6,7,8) glucose molecules
Cyclic oligosaccharide, there is very peculiar space structure -- columnar hollow-core construction.Due to being gone back inside and outside its cavity on " wall "
" extension " has the different groups on many glucose molecules, therefore the inside and outside of cyclodextrine cavity is had entirely different property：Outside
Table has hydrophily, and interior table has lipophile.So, by its " dual character ", to be difficult to set up between molten altogether oil and water
One " bridge ".
EGF/ vitamin e acetates-application of the cyclodextrin liposome in cosmetics of the present invention：By the EGF/ of the present invention
Vitamin e acetate-cyclodextrin liposome, which is added in cosmetics, is made emulsion, eye cream, nourishing cream, massage cream, facial gel,
Eye essence, eye gel, cleansing cream, mildy wash, foundation cream, body lotion, body frost, body gels etc..The present invention's
EGF/ vitamin e acetates-cyclodextrin liposome can play anti-aging well and reparation skin for preparing cosmetics
Effect, addition are 0.1~10%.
Below, using specific embodiment to EGF/ vitamin e acetates-cyclodextrin liposome of the present invention and preparation method thereof
Etc. being described in further detail.
Embodiment 1
(1) 4.00g beta-schardinger dextrin is weighed, adding 100ml distilled water makes it fully dissolve；Separately take vitamin E acetic acid
The dispersion liquid of inclusion compound is centrifuged into 60min under 70000g after ester 0.50g, 35 DEG C of stirring 6h, supernatant is vitamin E acetic acid
The aqueous solution of ester-cyclodextrin inclusion compound, supernatant is concentrated in vacuo, and concentrate dries 6h in 40 DEG C of vacuum drying chamber, 0.08MPa
Powdered vitamin e acetate-cyclodextrin inclusion compound is produced, reaches 86.57% by determining inclusion rate.
(2) 10.25g soybean lecithins are mixed with 2.50g cholesterol and be dissolved in 50ml absolute ethers；By 1.50gEGF and
Said vitamin E acetic acid esters-cyclodextrin inclusion compound 3.00g is dissolved in 50ml 0.02M pH7.4 phosphate buffer solution.
After solution is sufficiently mixed, under the conditions of ice-water bath be ultrasonically treated (ultrasonic power 80%, ultrasonic time 5min), after rotation
Removal of solvent under reduced pressure in evaporimeter, after solvent is evaporated completely, stops the rotation and keep vacuum to continue to stop after aspirating 30min, add
Enter 50ml 0.02M pH7.4 phosphate buffer solution, rotate the gel being hydrated in bottle wall and come off, that is, obtain inclusion compound fat
Plastid suspension, the suspension is used into high pressure homogenizer homogeneous 5 times.
(3) 5.00g trehaloses are added in above liposome turbid liquor, fully by inclusion liposome suspension after dissolving
It is poured into plastic culture dish and is freeze-dried, lyophilisation condition is：Pre-freezing temperature -60 ± 2 DEG C, carried out using quick-frozen mode, pre-freeze
Time 6h, condenser temperature -50~-55 DEG C, vacuum ＜ 100mT, freeze-drying time 24h, produce powdered EGF/ vitamin Es acetic acid
Ester-cyclodextrin liposome.Deionized water aquation is added to rebuild during use, gently concussion dissolving produces the inclusion compound fat after rebuilding completely
Plastid suspension, using supercentrifugation, measure envelop rate is 50.16%.
Embodiment 2
(1) 3.80g hydroxypropyl-β-cyclodextrin is weighed, adding 100ml distilled water makes it fully dissolve, and separately takes dimension to give birth to
The dispersion liquid of inclusion compound is centrifuged into 60min under 70000g after plain E acetic acid esters 0.70g, 30 DEG C of stirring 4h, supernatant is vitamin
The aqueous solution of E acetic acid esters-cyclodextrin inclusion compound, supernatant is concentrated in vacuo, and concentrate is in 40 DEG C of vacuum drying chamber, 0.08MPa
Dry 6h and produce powdered vitamin e acetate-cyclodextrin inclusion compound, reach 82.53% by determining inclusion rate.
(2) 10.25g egg yolk lecithins are mixed with 2.50g cholesterol and be dissolved in 50ml absolute ethers；By 1.50gEGF and
In the phosphate buffer solution for the pH7.4 that said vitamin E acetic acid esters-cyclodextrin inclusion compound 3.00g is dissolved in 50ml 0.02M.Will
After solution is sufficiently mixed, under the conditions of ice-water bath be ultrasonically treated (ultrasonic power 80%, ultrasonic time 5min), after rotation steam
Removal of solvent under reduced pressure in instrument is sent out, after solvent is evaporated completely, stops the rotation and keeps vacuum to continue to stop after aspirating 30min, add
50ml 0.02M pH7.4 phosphate buffer solution, rotate the gel being hydrated in bottle wall and come off, that is, obtain inclusion compound lipid
Body suspension, the suspension is used into high pressure homogenizer homogeneous 4 times.
(3) 5.00g mannitol and 2.00g dextrans are added in above liposome turbid liquor, will fully after dissolving
Inclusion liposome suspension, which is poured into plastic culture dish, to be freeze-dried, and lyophilisation condition is：Pre-freezing temperature -60 ± 2 DEG C, use
Quick-frozen mode is carried out, and pre-freeze time 6h, condenser temperature -50~-55 DEG C, vacuum ＜ 100mT, freeze-drying time 24h, produces powder
Shape EGF/ vitamin e acetates-cyclodextrin liposome.Deionized water aquation is added to rebuild during use, gently concussion dissolving is completely
Inclusion liposome suspension after must rebuilding, using supercentrifugation, measure envelop rate is 54.98%.
Embodiment 3
(1) 4.50g beta-schardinger dextrin is weighed, adding 100ml distilled water makes it fully dissolve, and separately takes vitamin E acetic acid
The dispersion liquid of inclusion compound is centrifuged into 60min under 70000g after ester 0.90g, 50 DEG C of stirring 5h, supernatant is vitamin E acetic acid
The aqueous solution of ester-cyclodextrin inclusion compound, supernatant is concentrated in vacuo, and concentrate dries 6h in 40 DEG C of vacuum drying chamber, 0.08MPa
Powdered vitamin e acetate-cyclodextrin inclusion compound is produced, reaches 86.23% by determining inclusion rate.
(2) 10.25g hydrogenated soya phosphatides are mixed with 2.50g cholesterol and be dissolved in 50ml absolute ethers；By 1.50gEGF
In said vitamin E acetic acid esters-cyclodextrin inclusion compound 3.00g pH7.4 for being dissolved in 50ml 0.02M phosphate buffer solution.
After solution is sufficiently mixed, under the conditions of ice-water bath be ultrasonically treated (ultrasonic power 80%, ultrasonic time 5min), after rotation
Removal of solvent under reduced pressure in evaporimeter, after solvent is evaporated completely, stops the rotation and keep vacuum to continue to stop after aspirating 30min, add
Enter 50ml 0.02M pH7.4 phosphate buffer solution, rotate the gel being hydrated in bottle wall and come off, that is, obtain inclusion compound fat
Plastid suspension, the suspension is used into high pressure homogenizer homogeneous 5 times.
(3) 4.00g sorbierite, 4.00g trehaloses, 2.00g xylitols are added in above liposome turbid liquor, fully
Inclusion liposome suspension is poured into plastic culture dish after dissolving and is freeze-dried, lyophilisation condition is：Pre-freezing temperature -60 ±
2 DEG C, carried out using quick-frozen mode, pre-freeze time 6h, condenser temperature -50~-55 DEG C, vacuum ＜ 100mT, freeze-drying time 24h,
Produce powdered EGF/ vitamin e acetates-cyclodextrin liposome.Add deionized water aquation to rebuild during use, gently shake molten
Solution produces the inclusion liposome suspension after rebuilding completely, and using supercentrifugation, measure envelop rate is 51.98%.
Test example 1
EGF/ vitamin e acetates-cyclodextrin liposome stability experiment
By EGF/ vitamin e acetates-cyclodextrin liposome of embodiment 1~3 in temperature 45 C, relative humidity 60%
Closed placement under part, 0 after placement, 1,2, March, respectively determine liposome in the content of EGF and vitamin e acetate, with 0 month
When liposome in the content of EGF and vitamin e acetate be respectively 100%, other each time coenzyme EGF and vitamin E acetic acid
The content of ester is made comparisons therewith respectively, observes the situation that the content of EGF and vitamin e acetate changes over time.
As a result find, under the conditions of 40 DEG C of temperature, relative humidity 75%, place 3 months, the EGF and dimension of embodiment 1~3
In raw plain E acetic acid ester liposome, EGF and vitamin e acetate changes of contents in liposome are little, and stability is good, and keep
Good biological activity, illustrate the EGF and vitamin e acetate liposome of the present invention, the selection of its liposome membrane material and use
Amount optimization, serves the effect for improving EGF and vitamin e acetate stability, and maintain biological activity well really.
Test example 2
EGF and vitamin e acetate-cyclodextrin liposome using effect experiment
The object of observation：25~45 years old age volunteer.
Choosing face has the women 100 of the problems such as color spot, wrinkle, relaxation, acne, damaging skin skin, is divided into 4 groups,
Every group 25.
Experimental method：Investigate respectively do not added in EGF and vitamin e acetate, skin cream in skin cream directly addition and
Only add directly to add in EGF sterlings, skin cream and only add and embodiment 1 is added in vitamin e acetate sterling and skin cream
Middle EGF/ vitamin e acetates-cyclodextrin liposome is in the case of these four, and observation skin cream is to making color spot, wrinkle, relaxation, Cuo
The improvement situation of several aspects such as sore, damaging skin simultaneously carries out periodic information feedback.
Before sleep every night after cleaning skin, skin cream is coated.It is a course for the treatment of to be used continuously 28 days.
Criterion of therapeutical effect：
(1) it is effective：Up to more than 90%, skin has obvious white for the reduction of spot acne wrinkle, elasticity enhancing, damaging skin repair
Fair-skinned moist smooth feeling；
(2) effectively：Up to more than 50%, skin is more pale moist for the reduction of spot acne wrinkle, elasticity enhancing, damaging skin repair
It is smooth；
(3) it is invalid：It is unchanged before and after treatment.
As a result：After 30 days, first group uses the volunteer's skin for not adding EGF and vitamin e acetate skin cream
Substantially it is unchanged；
Second and the 3rd group using addition EGF or vitamin e acetate skin cream, 30% volunteer's skin light it is gorgeous it is moist,
Pale, elasticity enhancing, wrinkle are reduced；
4th group uses addition EGF and vitamin e acetate cyclodextrin liposome skin cream, and 78% volunteer is on the face
Wrinkle almost eliminate, skin it is bright and clean moisten, color spot and freckle substantially eliminate, skin elasticity greatly enhances, and the use that to have a competition
The preceding obvious youth that seems.
Thus illustrate, EGF/ of the invention vitamin e acetate-cyclodextrin liposome can actually play anti-ageing to skin
Always, wrinkle and repair are desalinated, and the liposome of the present invention is than being used alone EGF sterlings and vitamin e acetate sterling effect
Fruit is more preferably obvious, and is added into skin care item or cosmetics the effect of also contributing to improve skin care item or cosmetics, effect
Fruit is satisfactory.
The present invention can be summarized with others without prejudice to the concrete form of the spirit or essential characteristics of the present invention.Therefore, nothing
By from the point of view of which point, the embodiment above of the invention can only all be considered the description of the invention and can not limit this hair
Bright, claims indicate the scope of the present invention, and above-mentioned explanation does not point out the scope of the present invention, therefore, with this
Any change in claims of invention suitable implication and scope, all it is considered as the claim for being included in the present invention
The scope of book.
Claims (10)
1. a kind of EGF/ vitamin e acetates-cyclodextrin liposome, it is characterised in that its raw material forms in parts by weight, bag
Include EGF stoste 2-50 parts, vitamin e acetate 2-50 parts, cyclodextrin or cyclodextrine derivatives 30-100 parts, phospholipid material 100-
220 parts, cholesterol 20-70 parts, freeze drying protectant 40-155 parts.
2. EGF/ vitamin e acetates-cyclodextrin liposome as claimed in claim 1, it is characterised in that the cyclodextrin is
Beta-schardinger dextrin or hydroxypropyl-β-cyclodextrin.
3. EGF/ vitamin e acetates-cyclodextrin liposome as claimed in claim 2, it is characterised in that the phospholipid material
Selected from soybean lecithin, egg yolk lecithin, hydrogenation yolk phospholipid, hydrogenated soya phosphatide, two palm phosphatidyl glycerols, distearyl phosphorus
Phosphatidyl glycerol, dilauroyl lecithin, two mustard acyl group lecithin, two nutmeg phosphatidyl glycerols, two palmityl phosphatidyl courages
At least one of alkali and DSPC.
4. EGF/ vitamin e acetates-cyclodextrin liposome as claimed in claim 3, it is characterised in that the frozen-dried protective
Agent is at least one of trehalose, mannitol, sorbierite, raffinose, dextran and lactose.
5. a kind of preparation method of EGF/ vitamin e acetates-cyclodextrin liposome as described in claim any one of 1-4,
Characterized in that, step is as follows：
(1) cyclodextrin or cyclodextrine derivatives are added to the water by proportioning, stirring and dissolving, it is 5~60% to obtain mass concentration
Cyclodextrin or cyclodextrine derivatives solution；
(2) vitamin e acetate is slowly added dropwise to the cyclodextrin or cyclodextrine derivatives solution obtained to step (1) by proportioning
In, strong stirring 1-6h at a temperature of 20-60 DEG C, obtain inclusion compound dispersion liquid；
(3) the inclusion compound dispersion liquid that step (2) obtains is centrifuged into 60min under centrifugal force 70000g, it is after standing that supernatant is true
Sky concentration, then concentrate is obtained into powdered vitamin e acetate-cyclodextrin bag in 40 DEG C of dry 3-6h of vacuum drying chamber
Compound；
(4) vitamin e acetate-cyclodextrin inclusion compound for obtaining EGF stostes and step (3) by proportioning is as core, then presses
Proportioning adds phospholipid material, cholesterol is coated, and obtains EGF/ vitamin e acetates-cyclodextrin liposome turbid liquor；
(5) the EGF/ vitamin e acetates-cyclodextrin liposome turbid liquor obtained step (4) is freeze-dried, obtains this hair
Bright EGF/ vitamin e acetates-cyclodextrin liposome.
6. the preparation method of EGF/ vitamin e acetates-cyclodextrin liposome as claimed in claim 5, it is characterised in that institute
Stating step (4) is specially：EGF stostes and vitamin e acetate cyclodextrin inclusion compound are dissolved in 0.02M pH7.4's by proportioning
In phosphate buffer solution, solution A is obtained；Phospholipid material is mixed with cholesterol and is dissolved in ether, obtains solution B；By solution A
It is added dropwise in solution B, is ultrasonically treated under the conditions of ice-water bath, then removal of solvent under reduced pressure in Rotary Evaporators, treat that solvent is evaporated completely
Afterwards, stop the rotation and keep vacuum to continue to stop after aspirating 30min, the phosphate-buffered for then adding 0.02M pH7.4 is molten
Liquid, the gel being hydrated in bottle wall come off, and using high pressure homogenizer homogeneous 4-5 times, obtain EGF/ vitamin e acetates-ring paste
Smart liposome turbid liquor.
7. the preparation method of EGF/ vitamin e acetates-cyclodextrin liposome as claimed in claim 6, it is characterised in that institute
The lyophilisation condition for stating step (5) is：Carried out using quick-frozen mode, pre-freezing temperature -60 ± 2 DEG C, pre-freeze time 6h, condenser temperature -
50~-55 DEG C, vacuum ＜ 100mT, freeze-drying time 24h.
8. a kind of application method of EGF/ vitamin e acetates-cyclodextrin liposome, it is characterised in that using such as claim
The preparation method of EGF/ vitamin e acetates-cyclodextrin liposome described in any one of 5-7 is prepared；The EGF/ dimensions life
The application method of plain E acetic acid esters-cyclodextrin liposome, in use, plus deionized water aquation rebuild, gently concussion dissolving is complete,
Obtain the inclusion liposome suspension after rehydration is rebuild, use.
9. the application method of EGF/ vitamin e acetates-cyclodextrin liposome as claimed in claim 8, it is characterised in that institute
The particle diameter for stating EGF/ vitamin e acetates-cyclodextrin liposome in the inclusion liposome suspension after rehydration is rebuild is 90-
200nm。
A kind of 10. EGF/ vitamin e acetates-application of the cyclodextrin liposome in cosmetics.
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