CN107788534A - 一种通过包埋技术制备的多菌共生益生菌微胶囊 - Google Patents
一种通过包埋技术制备的多菌共生益生菌微胶囊 Download PDFInfo
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Abstract
本发明涉及一种可以避免肠道内多菌共生益生菌遭受胃酸、胆盐攻击，提高多菌共生益生菌活菌数的通过包埋技术制备的多菌共生益生菌微胶囊，制备过程依次包括以下步骤：首先，筛选具有一定耐受性的菌株，并在环境压力较为严苛的条件进行驯养；其次，驯养后的菌株与其他营养菌株混合形成多菌共生益生菌；最后通过包埋技术将多菌共生益生菌包覆其中制备成胶囊，本发明的通过包埋技术制备的多菌共生益生菌微胶囊借由保护剂的筛选，将多菌共生益生菌包覆其中，使多菌共生益生菌在体内消化道输送过程中能够减轻受到胃酸、胆盐的伤害，增加到达小肠的活菌数，同时能够兼具维持多菌共生益生菌活性及菌数的功能，而且生产的时间也较冷冻干燥法生产时间更短。
Description
技术领域
本发明涉及一种多菌共生益生菌胶囊，尤其涉及一种通过包埋技术制备的多菌共生益生菌微胶囊。
背景技术
人体消化道自然存在的多菌共生益生菌是非常复杂的，除了常见的ABS三种菌外，还有一些耐高温耐酸的乳酸菌，与其他的乳酸菌共生共存，形成益菌优势原理，即“菌种越多，存活力越佳”，每个人有不同的体质，不同的乳酸菌也有不同的特性，乳酸菌与药品最大的不同是同一种乳酸菌对不同人的反应通常也不太一样。有些人可能适合这些菌，有些人可能适合别的菌。多种乳酸菌的组合通常可以避免掉上述的差异，让这一个乳酸菌的组合对大多数的人都有作用。但是并不是种类越多越好，而是适当的组合，这个组合的菌种彼此没有拮抗，而且最好能有协同作用。
人体肠道内存在的细菌，其中包含了有利于人体健康的多菌共生益生菌，也有易诱发疾病的坏菌。当肠道的益菌总数越多，坏菌数量就会相对减少，而栖息于肠道内的多种多菌共生益生菌会互助共生，增加彼此的功能与繁殖力，使坏菌数量大为减少，因此若能同时补充多种多菌共生益生菌配方，对肠道健康将有明显助益。然而复合式的菌种配方，需兼顾菌种间的共生活性与保存稳定性，这将是制程技术上的一大考验。
微胶囊技术最初为美国National Cash Register公司于1930年首创，在1954年正式引进用于生产无碳复写纸。微胶囊技术是利用一些可形成膜的物质将固体、液体或是气体原料包覆在密闭空间内的一种技术，可用来改变核心物质的颜色、形状、重量、溶解度以及对光、热、压力、空气的敏感性。目前已广泛应用于食品、香料、药品、化妆品、清洁剂等产品当中。制备微胶囊的方法有冷冻干燥法和喷雾干燥法。
而多菌共生益生菌产品的需求-多菌共生益生菌进入人体后，还必须经过胃酸、胆盐的攻击之后，才能够进入肠道进行定殖，因此必须能够在经过胃之后，仍有一定的活菌数，多菌共生益生菌必须经过食品加工之后仍具有一定的活菌数，同时在之后货架贮藏期间，仍能够维持一定的活菌数，而多菌共生益生菌产品在经过加工、运输、储存的过程中，容易造成菌数的快速流失，现有技术中一般采用冷冻干燥法和喷雾干燥法制备微胶囊保持多菌共生益生菌的活菌数，但是冷冻干燥法虽可保持多菌共生益生菌的活性，且含菌量高，但是粉体容易吸湿，不易保存，且费时成本高而喷雾干燥法虽可控制粉体特性，含菌量较冷冻干燥法低，且存活率较差。
所以有鉴于上述的缺陷，本设计人积极加以研究创新，以期创设一种新型的通过包埋技术制备的多菌共生益生菌微胶囊，使其更具有产业上的利用价值。
发明内容
为解决上述技术问题，本发明的目的是提供一种可以避免肠道内多菌共生益生菌遭受胃酸、胆盐攻击，提高多菌共生益生菌活菌数的通过包埋技术制备的多菌共生益生菌微胶囊。
本发明的通过包埋技术制备的多菌共生益生菌微胶囊，其制备过程依次包括以下步骤：首先，筛选具有一定耐受性的菌株，并在环境压力较为严苛的条件进行驯养；其次，驯养后的菌株与其他营养菌株混合形成多菌共生益生菌；最后通过包埋技术将多菌共生益生菌包覆其中制备成胶囊。
进一步的，所述环境压力包括温度、光照、水分、酸碱度等因素。
进一步的，其他营养菌株包括A菌(L.acidophilus)、B菌(Bifidobacteriumspecies)、C菌(Lactobacillus casei)、LGG菌(Lactobacillus rhamnosus GG)、LP菌(Lactobacillus paracasei)、B.subtilis、S.thermophilus、B.bereve、B.bifidus、L.bulgaricus、L.plantarum、L.reuteri。
进一步的，在培养完成的多菌共生益生菌中成功鉴定出8株菌株，培养液中可鉴定其他包括B.subtilis、S.thermophilus、B.bereve、B.Bifidus在内的4株菌株。
进一步的，所述包埋技术是借亲水性及疏水性芯材，配置成为水相均匀溶液与水包油乳液，在借由喷雾干燥将其多菌共生益生菌成品制作成胶囊借由上述方案，本发明至少具有以下优点：
由于微胶囊技术可以改变物质形态、质量和体积，提高其储存稳定性、溶解度和流动性；保护敏感性成分，大幅度提高对光、热、氧气和湿度等环境的抵抗能力；隔离活性物质或相互易反应的组成分；降低或遮盖不良的味道、色泽；降低挥发性、延长风味物质的滞留期；延缓食品的腐败变质；使不相溶成分混合、并降低某些化学添加剂的毒性等特性，多菌共生益生菌微胶囊包埋技术包含了现有技术中利用冷冻干燥法和喷雾干燥法制备微胶囊的优点，本发明的通过包埋技术制备的多菌共生益生菌微胶囊借由保护剂的筛选，将多菌共生益生菌包覆其中，使多菌共生益生菌在体内消化道输送过程中能够减轻受到胃酸、胆盐的伤害，增加到达小肠的活菌数，同时能够兼具维持多菌共生益生菌活性及菌数的功能，而且生产的时间也较冷冻干燥法生产时间更短。
上述说明仅是本发明技术方案的概述，为了能够更清楚了解本发明的技术手段，并可依照说明书的内容予以实施，以下以本发明的较佳实施例详细说明如后。
具体实施方式
下面结合实施例，对本发明的具体实施方式作进一步详细描述。以下实施例用于说明本发明，但不用来限制本发明的范围。
本发明一较佳实施例所述的一种通过包埋技术制备的多菌共生益生菌微胶囊，其制备过程依次包括以下步骤：首先，筛选具有一定耐受性的菌株，并在包括温度、光照、水分、酸碱度等环境因素压力较为严苛的条件进行驯养；其次，驯养后的菌株与其他营养菌株混合形成多菌共生益生菌；最后通过包埋技术将多菌共生益生菌包覆其中制备成胶囊。
其他营养菌株包括A菌(L.acidophilus)、B菌(Bifidobacterium species)、C菌(Lactobacillus casei)、LGG菌(Lactobacillus rhamnosus GG)、LP菌(Lactobacillusparacasei)、B.subtilis、S.thermophilus、B.bereve、B.bifidus、L.bulgaricus、L.plantarum、L.reuteri。
在培养完成的多菌共生益生菌中多菌共生益生菌成功鉴定出8株菌株，培养液中可鉴定其他包括B.subtilis、S.thermophilus、B.bereve、B.Bifidus在内的4株菌株。
包埋技术是借亲水性及疏水性芯材，配置成为水相均匀溶液与水包油乳液，在借由喷雾干燥将其多菌共生益生菌成品制作成胶囊。
本发明的工作原理如下：
包埋技术是利用β环糊精(β-cyclodextrin)作为载体，在分子等级下与芯材进行包合的微胶囊技术。因为β环糊精具有特殊的环状及空心结构，是由7个葡萄糖分子以α-1，4连结而成的环状化合物，外形为圆台状，内部则形成疏水中心，可与芯材分子结合使其置于中心部位完成包埋过程。其制程较为简易，只需配置饱和β环糊精水溶液，再依比例加入非极性的芯材充分搅拌后即可形成包合微胶囊。
由于微胶囊技术可以改变物质形态、质量和体积，提高其储存稳定性、溶解度和流动性；保护敏感性成分，大幅度提高对光、热、氧气和湿度等环境的抵抗能力；隔离活性物质或相互易反应的组成分；降低或遮盖不良的味道、色泽；降低挥发性、延长风味物质的滞留期；延缓食品的腐败变质；使不相溶成分混合、并降低某些化学添加剂的毒性等特性，通过包埋技术将多菌共生益生菌制备成多菌共生益生菌胶囊使多菌共生益生菌在体内消化道输送过程中能够减轻受到胃酸、胆盐的伤害，增加到达小肠的活菌数，同时能够兼具维持多菌共生益生菌活性及菌数的功能，而且生产的时间也较冷冻干燥法生产时间更短，经测试，通过包埋技术将多菌共生益生菌制备成多菌共生益生菌胶囊可于25°C储存6个月以上菌数保持在6x1010次方以上，可于37℃储存4个月菌数保持在2x1010以上，可抗胃酸达2小时以上，抗胆碱达3小时以上。
以上所述仅是本发明的优选实施方式，并不用于限制本发明，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明技术原理的前提下，还可以做出若干改进和变型，这些改进和变型也应视为本发明的保护范围。
Claims (5)
1.一种通过包埋技术制备的多菌共生益生菌微胶囊，其特征在于，其制备过程依次包括以下步骤：首先，筛选具有一定耐受性的菌株，并在环境压力较为严苛的条件进行驯养和筛选；其次，驯养后的菌株与其他营养菌株混合形成多菌共生益生菌；最后通过包埋技术将多菌共生益生菌包覆其中制备成胶囊。
2.根据权利要求1所述的一种通过包埋技术制备的多菌共生益生菌微胶囊，其特征在于，所述环境压力包括温度、光照、水分、酸碱度等因素。
3.根据权利要求1所述的一种通过包埋技术制备的多菌共生益生菌微胶囊，其特征在于，其他营养菌株包括A菌(L.acidophilus)、B菌(Bifidobacterium species)、C菌(Lactobacillus casei)、LGG菌(Lactobacillus rhamnosus GG)、LP菌(Lactobacillusparacasei)、B.subtilis、S.thermophilus、B.bereve、B.bifidus、L.bulgaricus、L.plantarum、L.reuteri。
4.根据权利要求4所述的一种通过包埋技术制备的多菌共生益生菌微胶囊，其特征在于，多菌共生益生菌成功鉴定出8株菌株，培养液中可鉴定其他包括B.subtilis、S.thermophilus、B.bereve、B.Bifidus在内的4株菌株。
5.根据权利要求1所述的一种通过包埋技术制备的多菌共生益生菌微胶囊，其特征在于，所述包埋技术是借亲水性及疏水性芯材，配置成为水相均匀溶液与水包油乳液，在借由喷雾干燥将其多菌共生益生菌成品制作成胶囊。
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| CN111690565A (zh) * | 2020-07-02 | 2020-09-22 | 重庆第二师范学院 | 一种益生菌及其在胃损伤中的应用 |
| CN116602932A (zh) * | 2023-02-28 | 2023-08-18 | 青岛康益生物科技有限公司 | 一种多菌共生颗粒及其制备方法和应用 |
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| CN116602932B (zh) * | 2023-02-28 | 2024-04-26 | 青岛康益生物科技有限公司 | 一种多菌共生颗粒及其制备方法和应用 |
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