CN107773275B - Skin detection sampling bag and application thereof - Google Patents

Skin detection sampling bag and application thereof Download PDF

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CN107773275B
CN107773275B CN201711154709.7A CN201711154709A CN107773275B CN 107773275 B CN107773275 B CN 107773275B CN 201711154709 A CN201711154709 A CN 201711154709A CN 107773275 B CN107773275 B CN 107773275B
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skin
sampling
film
kit
film coating
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CN107773275A (en
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何海鸥
马自竹
王德友
周立武
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Zhuhai Easycare Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/20—Dermatological disorders
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/20—Dermatological disorders
    • G01N2800/207—Pigmentation disorders

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Abstract

The invention discloses a kit for evaluating skin conditions, which is characterized by comprising a laminating kit for transcribing skin textures and a keratinocyte sampling detection tool; the film coating kit comprises a film coating frame, a film coating and a film coating paste, wherein a hole is formed in the middle of the film coating frame; the keratinocyte sampling and detecting tool comprises a loading plate and a transparent adhesive tape, wherein a collecting hole is formed in the loading plate, a chamfer is formed in one side of the collecting hole, an adhesive layer of the transparent adhesive tape is fixed on one side of the collecting hole without the chamfer, and the transparent adhesive tape completely covers the collecting hole; and synthesizing the observation results of the keratinocytes and the skin textures, and accurately analyzing to obtain the skin physiological condition information, so as to guide the development of a customized formula, thereby realizing personalized and accurate skin care.

Description

Skin detection sampling bag and application thereof
Technical Field
The invention belongs to the technical field of skin detection, and particularly relates to a skin detection sampling bag suitable for personalized customization of cosmetics and application thereof.
Background
With the development of economy, consumers have a higher and higher interest in skin health, and how to accurately evaluate skin conditions, and selecting appropriate skin care products is particularly important for consumers and cosmetic manufacturers.
At present, the main methods for skin test at home and abroad are as follows: 1. the skin image data are collected through an external image collection device and transmitted to a computer for analysis, the professional stable image collection device is large in size and inconvenient to carry, and although the mobile terminal (smart phone) is convenient to photograph, the stability and repeatability of light, photo quality and the like are poor; 2. various probe sensors, such as measuring pH value, melanin, sebum, elasticity, moisture and the like of skin, have huge detection precision differences with different prices, different detection standards, and the accuracy of the result is affected by environment and personnel operation. In either of these methods, the consumer is required to have equipment or be able to physically reach a location such as a chest or beauty shop to complete the skin test.
Keratinocytes are flat cells constituting the stratum corneum, in which many skin information is present, and skin conditions can be known by analyzing keratinocytes. Keratinocytes are located in the outermost layer of the skin, and thus, resist various external stimuli, including the skin; meanwhile, the binding force between the keratinocytes is weak, so that the keratinocytes are easy to collect. Chinese patent application CN2013100128496 discloses a cell encapsulation method and a cell observation method, in which cells are encapsulated between an adhesive layer of a transparent adhesive tape and an adhesive layer of the transparent adhesive tape, and then the encapsulated cells are observed by a phase difference microscope without using a staining solution; however, only the rough morphology of the cells can be observed by this method, and the appearance of nuclei and melanin is unclear. Moreover, by adopting the packaging method, bubbles are easy to generate in the operation process, and the detection result is further influenced.
The skin texture refers to a texture pattern appearing on the surface of human skin, also called dermatoglyph, and human skin is composed of epidermis and dermis. The dermal papilla protrudes toward the epidermis to form a number of aligned, parallel papilla lines, also known as ridge lines. The ridge lines have a plurality of sweat gland openings. The raised ridge grooves in turn form recessed grooves with each other. These concave-convex textures constitute the skin texture of the human body. The dermatoglyph of the human body has individual waiting specificity and high stability.
With age, the skin gradually ages, and the depth of skin texture deepens, and wrinkles occur due to aging or excessive insolation or repeated muscle movement. The skin texture is transcribed through the skin tectorial membrane, and indexes such as pores, sebum, elasticity and the like of the skin can be observed, so that the health condition of the skin can be estimated.
Currently, a skin texture test method on the market generally adopts a silica gel film coating to prepare a skin texture sample or a professional skin contour detector. The silica gel film coating is adopted to prepare the skin pattern sample, so that the defects of long drying time, few observation indexes (such as only observing texture, shallow skin groove) and the like exist; the skin texture data are acquired by the skin contour detector through the external image acquisition equipment and are transmitted to the computer for analysis, the professional stable image acquisition equipment is large in size and inconvenient to carry, and the mobile terminal (smart phone) is convenient to photograph, but poor in stability repeatability on light, photo quality and the like.
Therefore, a quick and convenient tool for evaluating skin conditions with low cost is needed, and a consumer can collect skin samples at home by himself, and accurate skin measurement is realized by analyzing the skin samples collected by the consumer.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a skin detection sampling package and a skin detection sampling method suitable for personalized customization of cosmetics.
A skin test sampling kit (as shown in fig. 1) comprising a dressing kit for transcribing skin texture, and a keratinocyte sampling test tool; the film coating kit comprises a film coating frame, a film coating and a film coating paste, wherein a hole is formed in the middle of the film coating frame; the keratinocyte sampling and detecting tool comprises a loading plate and a transparent adhesive tape, wherein a collecting hole is formed in the loading plate, a chamfer is formed in one side of the collecting hole, an adhesive layer of the transparent adhesive tape is fixed on one side of the collecting hole without the chamfer, and the transparent adhesive tape completely covers the collecting hole.
Preferably, the kit further comprises a catch plate.
Further preferably, 2 sockets are arranged on the clamping plate and used for respectively placing the complex film suite after the sampling and the keratinocyte sampling detection tool, so that the damage of the sample caused in the transferring process is avoided. In addition, two-dimensional codes or bar codes and the like can be stuck on the clamping plate so as to record information of a user.
Preferably, the kit further comprises a questionnaire for investigating user living habits, skin care habits, living environment, skin status and the like.
In the keratinocyte sampling detection tool:
preferably, the loading plate is made of PE, PC or PVC.
Preferably, the loading plate has a length of 65-90mm, a width of 15-40mm and a thickness of 1-5mm. The size of the loading plate adopted in the invention accords with the specification design of a conventional glass slide, so that the keratinocytes can be conveniently obtained and then directly dyed and observed under a microscope.
Preferably, the collecting hole is in any shape convenient for microscopic observation, such as a circle or a polygon, and preferably, the collecting hole is in a circle; the size of the collecting hole is 1-30mm. It will be appreciated that the diameter is 1-30mm when the collection well is circular and the side length is 1-30mm when the collection well is polygonal. The collecting hole is arranged at the middle upper part of the loading plate, which is more beneficial to the operation of collecting the keratinocytes.
Preferably, the chamfer is provided along the edge of the collection well. The height of the chamfer is 0.5-5mm, the width of the chamfer is 0.5-5mm, and the height and the width of the chamfer can be the same or different; more preferably, the chamfer has the same dimensions of height and width. The chamfering function is that: during dyeing, the dyeing liquid is dripped into the collecting hole, so that the dyeing liquid is fully contacted with the keratinocytes on the transparent adhesive tape, and the chamfer can prevent the dyeing liquid from overflowing the collecting hole, thereby having the effects of accurately dyeing, reducing the consumption of the dyeing liquid and the like; in addition, the chamfer is also beneficial to the operation of adding and flushing the dye liquor, and the dye liquor is convenient to pour out after dyeing is finished. The height of the chamfer can affect the sampling and staining effect. The chamfer angle is too low, which causes insufficient dyeing liquid and easy overflow of the dyeing liquid; too high chamfer angle can cause difficult sampling, even too small sampling amount so as to influence the detection effect; more preferably, the chamfer has a height of 0.5-2mm.
The transparent adhesive tape is safe, nontoxic and capable of directly contacting skin, preferably, the linear transmittance of the transparent adhesive tape is more than 80%, and the linear transmittance is too low to influence the observation definition of glial cells. Preferably, the 180-degree peel strength of the transparent adhesive tape is not more than 5.0N/25mm and not more than 10N/25mm. The transparent adhesive tape has overlarge viscosity, and can cause pain and damage to skin when being torn off; too low viscosity is detrimental to keratinocyte collection. The material of the film layer includes, but is not limited to, polyethylene, polypropylene, cellophane, polyethylene terephthalate, polyolefin, etc.
Further preferably, the transparent adhesive tape includes, but is not limited to, skin corneocyte exfoliation test film of CK company in germany, corneocyte exfoliation test film of Cuderm company in usa.
Preferably, a small hole with a diameter of 1-2mm is arranged beside the sampling hole. The small hole and the center of the sampling hole are on the same horizontal line. On one hand, the small holes play a role in positioning, and when keratinocytes are collected, the small holes are aligned to the intersection points of the canthus and the nasal wings, so that the consistency of the sampling area is convenient to be repeated; on the other hand, the small hole is stripped by a sampling tool after the sampling is finished, so that the skin is prevented from being damaged.
Preferably, the keratinocyte sampling test tool further comprises a protective sheath. The protective sleeve is a paper base material, a plastic base material and the like. The protective sleeve is hollow in the inside, two ends of the protective sleeve are open, and the protective sleeve is arranged on the loading plate; the inner diameter of the protective sleeve is set to be capable of sliding on the loading plate under the action of external force; for example, when sampling is prepared, the protective sleeve can be pushed to slide to the lower part of the loading plate, so that the collecting hole on the loading plate is completely exposed, and the sampling is convenient; after the sampling is finished, the protective sleeve can be pushed to slide and completely cover the collecting hole, so that the keratinocytes collected on the transparent adhesive tape can be prevented from being polluted by dust, bacteria and the like.
It is further preferred that a hole is provided on one side of the protective sleeve to facilitate observation of whether the sampling tool has sampled. The hole on the protective sleeve is close to one side of the film layer of the transparent adhesive tape on the loading plate, the size of the hole is that the sampling condition on the transparent adhesive tape can be completely observed, and the hole on the protective sleeve is preferably completely overlapped with the collecting hole.
In the film-coating kit for transferring skin texture:
preferably, the length of the film laminating frame is 10-30mm, the width of the film laminating frame is 10-30mm, and the thickness of the film laminating frame is 0.05-0.5mm. The holes on the film coating frame are round or polygonal. The size of the holes on the film coating frame is 5-25mm. The material of the film coating frame comprises, but is not limited to PE and PVC. Further preferably, an adhesive layer is provided on one side of the film coating frame, so that the film coating frame can be adhered to the skin. The film coating frame is used for preventing the formed film coating from being too large or too small to influence microscopic observation and playing a role in positioning through the Kong Tuma film coating agent on the film coating frame when the film coating agent is coated.
Preferably, the coating is formed by coating a coating agent on the skin and then curing.
Preferably, the laminating agent is a substance that can transcribe skin texture after curing, such as a silicone film.
Further preferably, the film laminating agent is a film laminating composition comprising the following raw materials in weight ratio:
6-35% of film forming agent,
1-20% of filler,
0.1-10% of auxiliary agent,
the balance being solvent.
The composite film composition is prepared by the following steps:
(1) Sequentially adding a film forming agent and a filler into a solvent, heating to 70-90 ℃, and uniformly stirring;
(2) Cooling to room temperature, adding the auxiliary agent, and stirring uniformly.
Preferably, the auxiliary agent is at least one selected from the group consisting of grease, thickener, emulsifier, anti-sensitization agent and preservative.
More preferably, the laminating composition comprises the following components in weight percent:
preferably, the film forming agent is at least one selected from polyvinyl alcohol, polyvinylpyrrolidone, acrylic acid (ester) copolymer/ethylhexyl acrylate copolymer, styrene/acrylic acid (ester) copolymer, polyurethane-35.
Preferably, the filler is at least one selected from titanium dioxide, kaolin, zinc oxide, talcum powder, diatomite, mica powder, silica, silicon dioxide, bamboo charcoal powder, quartz powder, attapulgite, bentonite, organobentonite, pozzolan, spaghetti, silt, activated clay, hydrohalite, magnesium carbonate and montmorillonite.
Preferably, the thickener is at least one selected from cellulose, magnesium aluminum silicate, xanthan gum, carbomer, carrageenan.
Preferably, the grease is at least one selected from mineral oil, olive oil, jojoba oil, sweet almond oil, caprylic/capric triglyceride, ethylhexyl palmitate, hydrogenated polydecene, isohexadecane, sunflower (HELIANTHUS ANNUUS) seed oil, isopropyl palmitate, C12-15 alcohol benzoate, polydimethylsiloxane, cyclopentadimethicone.
Preferably, the emulsifier is selected from at least one of polyglycerol-3 stearate, polyglycerol-10 oleate, polyglycerol-4 laurate, monoglyceride, tween-60, coco glucoside, PEG-100 stearate, cetostearyl ether-6, cetostearyl ether-25, potassium cetyl phosphate.
Preferably, the antiallergic agent is at least one selected from allantoin, dipotassium glycyrrhizinate, and herba Portulacae extract.
Preferably, the preservative is at least one selected from phenoxyethanol, imidazolidinyl urea and hydantoin.
Preferably, the solvent is at least one selected from water, glycerol, propylene glycol, butylene glycol, glycerol polyether-26, alcohol.
The preparation method of the composite film composition for acquiring the physiological state of the skin comprises the following steps:
(1) Sequentially adding a film forming agent, a thickening agent and a filler into a solvent, heating to 70-90 ℃ within 10-20min, uniformly stirring at a stirring speed of 200-300r/min, and obtaining a phase A for later use;
(2) Mixing and stirring the grease and the emulsifying agent, and heating to 70-80 ℃ to obtain a phase B for standby;
(3) Adding phase B into phase A, homogenizing for 2min, cooling to 40-50deg.C, adding antiallergic agent and antiseptic, stirring, and dissolving.
More preferably, the laminating composition is in the form of a paste.
In the present invention, the film is filled in a container, preferably a hose, in the form of a film coating composition. When in use, the composite film composition is coated on skin, and after drying and curing, the composite film is formed.
Preferably, the film-forming composition is applied to a thickness of 0.05-0.5mm. The coating composition cannot be coated too thick, so that the coating composition is difficult to dry and cannot be transferred to a coating patch; the coating composition cannot be applied too thinly, resulting in an inability to clearly transcribe skin texture and an inability to fully demonstrate sebum secretion.
Preferably, the laminating adhesive comprises a transparent film layer, an adhesive layer and a protective layer which are sequentially connected.
The compound film is safe, nontoxic and can be directly contacted with skin.
Further preferably, the 180-degree peel strength of the adhesive layer is ∈5.0N/25mm or less and is not more than 10N/25mm; the adhesive layer has overlarge viscosity, and can cause pain and damage to skin when being torn off; the tackiness is too low to adhere and transfer the film, even if the film is broken during transfer. The transparent film layer is made of polyethylene, polypropylene, cellophane, polyethylene terephthalate, polyolefin, etc.
Further preferably, the protective layer is divided into 2 parts, and the intermediate part may entirely cover the coating film. More preferably, the protective layer is release paper, oiled paper, or the like.
The laminating external member still includes and examines the utensil.
Preferably, a hole is formed in the gauge, wherein the hole in the gauge is larger than or equal to the hole in the laminating frame, so that the integral lamination transcribed skin texture can be observed in a microscopic manner.
Further preferably, the gauge is 65-90mm long, 15-40mm wide and 0.5-5mm thick. The hole on the gauge is arranged at the middle upper part of the gauge. Further preferably, the material of the gauge is PE, PC, PVC or the like.
More preferably, the tail of the gauge is designed as a rounded corner (as shown in fig. 6), which can be used to apply the film-coating composition to the skin, avoiding contamination.
The sampling method of the keratinocyte sampling detection tool comprises the following steps:
(1) Pushing the protective sleeve to completely expose the collecting hole, and pressing one side of the tool with the chamfer angle on the skin;
(2) Pressing the collection hole with the finger abdomen; pressing the collection hole from top to bottom by finger belly for at least three times in a stroking mode;
(3) After sampling, the periphery of the canthus is pressed by hands, and the tool is slowly torn off from bottom to top;
(4) The protective sleeve is pushed to cover the side of the transparent adhesive tape where keratinocytes are collected.
A method of using a film-coating kit for transcribing skin texture, comprising the steps of:
(1) After cleaning the skin, wiping off the skin;
(2) Sticking the film coating frame on skin;
(3) Applying the film coating composition from Kong Tuma of the film coating frame to skin and uniformly coating;
(4) Tearing off the film coating frame, and keeping the film coating composition on the skin for 8-15min until the film coating composition is completely dried;
(5) Tearing off the middle part of the protective layer on the laminating adhesive, aligning the adhesive layer of the exposed part with the laminating adhesive and adhering the adhesive layer on the laminating adhesive, and lightly pressing the laminating adhesive to enable the laminating adhesive to be tightly adhered to the laminating adhesive;
(6) Slowly tearing off the compound film paste adhered with the compound film from top to bottom;
(7) Tearing off the laminated film and sticking the residual protective layer to align the laminated film with the hole on the checking fixture, and sticking the laminated film on the checking fixture.
Preferably, the middle position of the left side of the laminating frame is aligned with the intersection point of the nose and the corner of the eye at a position which is horizontally 5mm away from the corner of the eye.
It should be noted that the expression should be avoided to change during the drying and curing process of the film, otherwise the analysis result will be affected.
The method for evaluating the skin condition adopts the tool kit to sample, and then the sample is placed under a microscope to observe and analyze the skin condition. The complex film kit and the keratinocyte sampling and detecting tool in the kit are respectively and independently packaged before use and then are packaged in the same packaging bag together with the clamping plate and the questionnaire. When the kit is used, after unpacking, the kit and the keratinocyte sampling and detecting tool are used for sampling respectively, after sampling is finished, the kit and the tool are fixed in the socket of the clamping plate respectively, and after two-dimensional codes or bar codes are attached, the kit is packed and sent to a relevant detecting unit for detection and analysis.
The detection and analysis method of the keratinocyte sampling detection tool comprises the following steps:
(1) Sampling: collecting skin keratinocytes by using the keratinocyte sampling detection tool;
(2) Dyeing: dropwise adding ferrous sulfate solution onto the transparent adhesive tape with the chamfer on one side of the transparent adhesive tape with the keratinocyte sampling detection tool collecting hole, immersing keratinocytes on the transparent adhesive tape, heating by microwaves, and washing with distilled water, preferably three times for three minutes each time to remove the ferrous sulfate solution; dripping the core solid red from the side with the chamfer into the collecting hole for dyeing, and washing with tap water, preferably tap water for 1-2min;
(3) And (3) airing the tool treated in the step (2), and observing under a microscope to evaluate the skin condition.
Preferably, the concentration of the ferrous sulfate solution is 0.01-0.035g/ml.
Preferably, the temperature of the microwave heating is 50-100 ℃ and the time is 1-6min.
Preferably, the time of the nuclear solid red staining is 5-10min.
Preferably, the concentration of the core red is 0.1-0.2%.
Criteria for assessing skin condition:
(1) Placing the collecting hole of the tool after dyeing under a 40-time microscope, and evaluating the polymerization degree of the keratinocyte by observing the exfoliation mode and state of the keratinocyte;
the polymerization degree is good: even distribution, no polymerization, indicating that the skin is healthy (as shown in fig. 13);
polymerization degree is poor: severe polymerization, indicating skin inflammation or sensitization (as shown in fig. 14);
(2) Placing the collecting hole of the tool after dyeing under a 100 times of microscope, and observing the arrangement mode of the keratinocytes;
the arrangement mode is good: the overlapping width between cells is proper and the cells are arranged without gaps. The cell shape is clear, and is pentagonal or hexagonal. (as shown in fig. 15);
the arrangement mode is poor: the degree of overlap between cells varies with gaps. The shape is not uniform. The instructions are either irritating or easily dried. (as shown in fig. 16);
(3) Placing the collecting hole of the tool after dyeing under a 100 times of microscope, and combining with a picture processing software system to calculate the average area of keratinocytes, wherein the larger the area of the keratinocytes is, the slower the metabolism speed of skin is and the skin is in a aging trend;
generally, 20-34 years old: 580+ -20 μm 2 A/b; 35-49 years old: 610+ -20 μm 2 A/b; age 50 or older than 50: 670+ -20 μm 2 A/b;
(4) The collecting hole of the tool after dyeing treatment is placed under a 100-time microscope, and the number of keratinocytes CE is calculated by combining with a picture processing software system, wherein the ratio of rCE/CE and skin sensitivity show negative correlation, namely, the lower the ratio of rCE/CE is, the higher the sensitivity of the skin is, the worse the barrier function of the skin is, and the skin is more easily stimulated by the external environment; the rCE/CE ratio is more than or equal to 60 percent, which indicates that the skin is in a normal health state;
(5) And placing the collection hole of the tool after dyeing under a 100-time microscope to observe whether cell nuclei exist or not, wherein the cells reach the stratum corneum when not fully mature, and sometimes nuclear residues exist. When the core remains, the core can become easy to be stimulated, and care should be taken to be gentle and gentle;
(6) Placing the collecting hole of the tool after dyeing under a 400-time microscope, observing the cutin (5), placing the collecting hole of the tool after dyeing under a 400-time microscope, and observing the melanin condition in the cells of the cutin layer, wherein the melanin is an amino acid derivative and is in the body of each person. The age of 20-25 years is the active phase of melanin formation. They are present in the middle of the cells of the basal layer of the skin, but a substance called "melanomas", also called "pigment blasts". The pigment parent cell secretes the Mailanning pigment, when ultraviolet rays (B wave and A wave) are irradiated on the skin (the B wave is UVB acts on basal layers of the skin), the skin is in a self-protection state, and the ultraviolet rays stimulate the Mailaner Ning Sesu to activate the activity of tyrosinase so as to protect the skin cells. Dopa is in fact a precursor of melanin, which is released by oxidation of tyrosine. Melanin moves layer by layer through cell metabolism, and forms freckle, sunburn, black spot, etc. on the epidermis layer of skin.
By analyzing the amount and distribution of melanin in the keratinocytes, the susceptibility to future stains can be known.
The melanin index is good: melanin is uniformly distributed, and the amount of melanin is small, as shown in fig. 17;
the melanin index is poor: the melanin distribution was uneven and the amount of melanin was large as shown in fig. 18;
the melanin status of the stratum corneum can also be objectively assessed by analyzing the melanin content index (MCI (melanin content index)) in the stratum corneum cells, wherein the melanin content index is the melanin content in the tested cells.
MCI values less than 10.10 indicate no apparent melanin presentation at superficial parts of the stratum corneum; MCI values between 10.10 and 11.45 indicate that small amounts of melanin granules are present in the superficial parts of the stratum corneum; the MCI is greater than 11.45 and the melanin particles exhibit a cap-like structure, indicating that pigmentation is more severe.
Detection analysis standard of the complex film kit:
(1) Skin texture was observed at 10 x magnification: the skin furrows are obvious, the skin hills are arranged in a neat shape (triangle), and the skin is full and raised, which indicates that the skin texture condition is good, the skin is bright, the surface is moist and has tension and elasticity, as shown in fig. 7 (a); the skin furrows are shallow, unobvious, uneven, not raised, flat, indicating poor skin texture, darkness, dry surface, no tension and elasticity, as shown in fig. 7 (b);
(2) Pores were observed under 50 x mirror: the small and unobvious texture intersection points indicate small pores, small quantity and smooth and fine skin, as shown in fig. 8 (a); the points of intersection of the textures are more and more obvious, which means that pores are larger, the surface of the skin is uneven, tension is not generated, and makeup is easy to remove, as shown in fig. 8 (b);
(3) The enlargement of 100 shows that the concave holes on the complex film are more and bigger, which means that the sebum secretion is vigorous, the pores are bigger, and the skin is easy to form acne, as shown in fig. 9 (a); the number of concave holes on the composite film is moderate, which means that sebum secretion is moderate and skin grease is balanced, as shown in fig. 9 (b); the small holes on the film showed little sebum secretion, and the skin was dry, easy to itching and even painful, as shown in fig. 9 (c).
And synthesizing the observation results of the keratinocytes and the skin textures, and accurately analyzing to obtain the skin physiological condition information, so as to guide the development of a customized formula, thereby realizing personalized and accurate skin care.
The invention has the beneficial effects that:
(1) The tool kit is simple and convenient to sample, quick and easy to operate, and a consumer can operate the tool kit by himself;
(2) The tool kit can detect keratinocytes and skin textures at the same time, so that the skin condition is analyzed and estimated, and the observation indexes are more comprehensive and accurate.
Drawings
FIG. 1 is a perspective view of a kit of the present invention;
FIG. 2 is a top view of a lamination frame of the lamination suite;
FIG. 3 is a schematic illustration of the construction of a film laminating adhesive of the film laminating kit;
FIG. 4 is a schematic view of a protective layer of a lamination suite;
FIG. 5 is a schematic view of a gauge of a lamination kit;
FIG. 6 is a side view of a gauge of the lamination suite;
FIGS. 7a-7b are photomicrographs of skin texture;
FIGS. 8a-8b and are photomicrographs of skin pores;
figures 9a-9c are microscopic images of sebum secretion from the skin;
FIG. 10 is a schematic diagram of a keratinocyte sampling test tool;
FIG. 11 is a schematic diagram of a keratinocyte sampling test tool;
FIG. 12 is a schematic view of a protective sheath of a keratinocyte sampling test tool;
FIG. 13 is a polymerization of healthy skin keratinocytes;
FIG. 14 is a graph showing the aggregation of keratinocytes in a population suffering from skin inflammation or sensitivity;
FIG. 15 is an arrangement of healthy skin keratinocytes;
FIG. 16 is an arrangement of stimulated skin keratinocytes.
Fig. 17 shows little melanin and uniform skin tone;
FIG. 18 shows that there is increased melanin and uneven skin tone;
FIGS. 19a-19c are microscopic images of keratinocyte cells, respectively, under 40, 100, 400-fold microscope, of the keratinocyte sampling test tool after the treatment of example 7;
FIGS. 20a-20c are microscopic images of keratinocyte cells, respectively, under 40, 100, 400-fold microscope, of the keratinocyte sampling test tool after treatment of example 8;
FIGS. 21a-21c are microscopic images of keratinocyte cells, respectively, under 40, 100, 400-fold microscope, of the keratinocyte sampling test tool after the treatment of example 9.
Detailed Description
Specific embodiments of the present invention are described in detail below with reference to the drawings and examples. It should be understood that the detailed description and specific examples, while indicating and illustrating the invention, are not intended to limit the invention.
Example 1
The composite film for transferring skin texture comprises the following raw materials in percentage by weight:
8% of polyvinyl alcohol, 15% of acrylic acid (ester) copolymer, 13% of kaolin, 2% of mineral oil, 0.02% of cellulose, 2% of polyglycerol-3 stearate, 2% of anti-sensitizer, 0.3% of preservative and the balance of water.
The preparation method comprises the following steps: sequentially adding polyvinyl alcohol, acrylic acid (ester) copolymer, cellulose and kaolin into water, stirring and heating to 78 ℃, homogenizing for 2 minutes, cooling to 45 ℃, adding mineral oil, polyglycerol-3 stearate, an anti-allergic agent and a preservative, and stirring and dissolving uniformly.
Example 2
The composite film for transferring skin texture comprises the following raw materials in percentage by weight:
5% of polyvinyl alcohol, 20% of styrene/acrylic acid (ester) copolymer, 20% of titanium dioxide, 4% of olive oil, 1% of magnesium aluminum silicate, 0.01% of polyglycerol-4 laurate, 4% of anti-sensitizer, 0.5% of preservative and the balance of water.
The preparation method comprises the following steps: sequentially adding polyvinyl alcohol, styrene/acrylic acid (ester) copolymer, magnesium aluminum silicate and titanium pigment into water, stirring, heating to 80 ℃, homogenizing for 2 minutes, cooling to 45 ℃, adding olive oil, polyglycerol-4 laurate, an anti-allergic agent and a preservative, and stirring and dissolving uniformly.
Example 3
The composite film for transferring skin texture comprises the following raw materials in percentage by weight:
15% of acrylic acid (ester)/ethylhexyl acrylate copolymer, 1% of acrylic acid (ester) copolymer, 1% of zinc oxide, 0.01% of ethylhexyl palmitate, 0.05% of xanthan gum, 4% of polyglycerol-4 laurate, 0.1% of an anti-sensitizer, 0.5% of a preservative and the balance of glycerin.
The preparation method comprises the following steps: sequentially adding acrylic acid (ester)/acrylic acid ethylhexyl ester copolymer, acrylic acid (ester) copolymer, xanthan gum and zinc oxide into glycerol, stirring and heating to 75deg.C, homogenizing for 2min, cooling to 45deg.C, adding ethylhexyl palmitate, polyglycerol-4 laurate, anti-allergic agent and antiseptic, stirring and dissolving uniformly.
Example 4
A kit for assessing skin condition (as shown in fig. 1) comprising a set of film-coating for transcribing skin texture, a keratinocyte sampling test tool, and a cardboard;
as shown in fig. 2-4, the film coating kit for transcribing skin textures comprises a film coating frame 1, a film coating 2, a film coating paste 3 and a gauge 4, wherein a hole 11 is arranged in the middle of the film coating frame. The length of the film laminating frame 1 is 15mm, the width is 15mm, and the thickness is 0.5mm; the side length of the upper hole of the film coating frame is 11mm; the composite film frame is made of PVC; an adhesive layer is arranged on one side of the film laminating frame. The composite film 2 is formed by coating the composite film composition of the embodiment 2 on skin, drying and curing after 15min, and is arranged in a hose; the thickness of the complex film is 0.1mm. The laminating adhesive 3 comprises a transparent film layer 31, an adhesive layer 32 and a protective layer 33 which are sequentially connected; the protective layer 33 is divided into 2 parts, and one part is a middle part, which can completely cover the coating film. The 180-degree peel strength of the adhesive layer was 6N/25mm. The gauge 4 is provided with a hole 41, wherein the hole on the gauge is slightly larger than the hole 11 on the film coating frame. The length of the gauge 4 is 76mm, the width is 25mm, and the thickness is 1.5mm.
As shown in fig. 10-11, the keratinocyte sampling detection tool comprises a loading plate 5, a transparent adhesive tape 7, a small hole 51 and a protective sleeve 6, wherein a collection hole 52 (round or square) with the radius of 9mm is arranged on the loading plate 5, a chamfer 53 with the height and the width of 1mm is arranged on one side of the collection hole 52, an adhesive layer of the transparent adhesive tape 7 is fixed on the non-chamfer side of the collection hole 52, and the transparent adhesive tape 7 completely covers the collection hole 52. The loading plate 5 is a PC plate 76.2mm long, 25.48mm wide and 2mm thick, and the transparent adhesive tape 7 is a skin keratinocyte peeling test film of the company CK in Germany. The small hole 51 has a diameter of 1.35mm and is at the same height as the center of the collection hole 52 on the loading plate 5.
Example 5
The application method of the tool kit comprises the following steps:
(1) Sampling of the complex film kit for transcribing skin texture:
a. after cleaning the skin, wiping off the skin;
b. sticking one side of the film laminating frame provided with an adhesive layer on skin, and enabling the middle position of the left side of the film laminating frame to be aligned with the intersection point of the position which is 5mm away from the corner of eyes and the nose;
c. extruding the composite film into holes on a composite film frame, and uniformly coating the composite film or taking a proper amount of composite film from the tail of a gauge, and uniformly coating the composite film on the skin along the holes in the composite film frame;
d. tearing off the film coating frame, and keeping the film coating composition on the skin for 15min until the film coating composition is completely dried to form a film coating;
e. taking a laminating adhesive 3, tearing the middle part of the protective layer 33, aligning the exposed adhesive layer with the laminating adhesive 2, adhering the adhesive layer and the laminating adhesive to the laminating adhesive, and completely transferring the laminating adhesive to the laminating adhesive;
f. slowly tearing off the compound film paste adhered with the compound film from top to bottom;
g. tearing off the laminated film and sticking the residual protective layer to align the laminated film with the hole on the gauge, and sticking the laminated film on the gauge;
(2) Sampling by using a keratinocyte sampling detection tool, namely pressing one side of the keratinocyte sampling detection tool with a chamfer 53 on the skin, aligning the small hole 51 with the intersection point of the canthus and the nasal wings, and pressing the collection hole 52 from top to bottom three times in a stroking mode by using the abdomen of the finger; the periphery of the canthus is pressed by hands, and the tool is slowly torn off from bottom to top, and a protective sleeve is covered;
(3) And respectively inserting the film coating kit for completing the sampled transcribed skin texture and the keratinocyte sampling detection tool into the clamping plate, and then filling the clamping plate into a package for the next detection and analysis.
Example 6
The film coating kit for transcribing skin textures after the sampling is completed can be directly observed under a microscope with 10-100 times of magnification by a gauge attached with a film coating;
and (3) carrying out microscope observation after the sampled keratinocyte sampling detection tool is subjected to dyeing treatment. Wherein the dyeing treatment comprises: (1) ferrous sulfate staining: dropwise adding a ferrous sulfate solution with the concentration of 0.01g/ml into the collection hole 52 from one side with the chamfer 53, heating by microwaves at the temperature of 70 ℃ for 4 minutes, washing by distilled water for three times within 3 minutes; (2) nuclear solid red counterstain: the core solid red is dripped into the collecting hole 52 from the side with the chamfer 53, and after 7min of dyeing, the core solid red is washed for 2min by tap water and dried. The state of the keratinocytes was then observed under 40-fold, 100-fold and 400-fold micromirrors, respectively.
Example 7
Volunteer a, female, age 30, were evaluated for skin condition using the kit of the present invention, with the results obtained:
the state of the keratinocytes is observed, and each index is shown in figure 19, the keratinocytes of the volunteer are seriously polymerized, are arranged in a moderate mode, are relatively sensitive to skin, have no cell nucleus, have good melanin indexes and have small melanin amount;
observing the composite film, the skin texture of the volunteer is better, and the skin sulcus is more obvious; the pore index is good, the pores are small, and the number of pores is small; the sebum is drier and the sebum secretion is less.
Example 8
Volunteers b, women, age 40, were evaluated for skin condition using the kit of the present invention, with the results obtained:
the state of the keratinocytes is observed, each index is shown in figure 20, the keratinocytes of the volunteer are seriously polymerized, the arrangement mode is poor, the skin is very sensitive, no cell nucleus exists, the index of melanin is medium, and the melanin amount is relatively small;
observing the composite film, wherein the skin texture of the volunteer is poor and the skin sulcus is shallow; the pore index is good, pores are fewer, and the number of pores is smaller; the sebum is drier and the sebum secretion is less.
Example 9
Volunteers, women, age 45, evaluate skin condition using the kit of the present invention, with the result:
the state of the keratinocytes is observed, and each index is shown as figure 21, the keratinocytes of the volunteer have moderate polymerization degree, poor arrangement mode, sensitive skin, no cell nucleus, poor melanin index and more melanin;
observing the composite film, wherein the skin texture of the volunteer is poor, the skin sulcus is shallow and is not obvious; the pore index is good, pores are fewer, and the number of pores is smaller; dry sebum and little sebum secretion.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that modifications and improvements could be made by those skilled in the art without departing from the inventive concept, which fall within the scope of the present invention.

Claims (8)

1. A skin test sampling kit comprising a set of membranes for transcribing skin texture, and a keratinocyte sampling test tool; the film coating kit comprises a film coating frame, a film coating and a film coating paste, wherein a hole is formed in the middle of the film coating frame; the keratinocyte sampling and detecting tool comprises a loading plate and a transparent adhesive tape, wherein a collecting hole is formed in the loading plate, a chamfer is formed in one side of the collecting hole, an adhesive layer of the transparent adhesive tape is fixed on one side of the collecting hole without the chamfer, and the transparent adhesive tape completely covers the collecting hole;
the compound film is formed by coating a compound film agent on skin, and then drying and curing, wherein the compound film agent comprises the following components in percentage by weight:
6-35% of film forming agent,
1-20% of filler,
0.1-10% of auxiliary agent,
the balance being solvent;
wherein the film forming agent is at least one selected from polyvinyl alcohol, polyvinylpyrrolidone, acrylic acid (ester) copolymer/ethyl hexyl acrylate copolymer, styrene/acrylic acid (ester) copolymer and polyurethane-35;
the filler is at least one selected from titanium dioxide, kaolin, zinc oxide, talcum powder, diatomite, mica powder, silica, silicon dioxide, bamboo charcoal powder, quartz powder, attapulgite, bentonite, organic bentonite, volcanic ash, hot spring soil, silt, activated clay, diaspore powder, magnesium carbonate and montmorillonite.
2. The skin test sampling kit of claim 1, wherein the kit further comprises a cardboard.
3. The skin test sampling kit of claim 2, wherein the card is provided with at least 2 sockets for placing the complete sampling kit and the keratinocyte sampling test tool, respectively.
4. The skin test sampling kit of claim 1, wherein in the keratinocyte sampling test tool, the chamfer is disposed along the edge of the collection well, the chamfer has a height of 0.5-5mm and a width of 0.5-5mm.
5. The skin test sampling kit according to claim 1, wherein in the keratinocyte sampling test tool, a small hole having a diameter of 1-2mm is provided beside the collection hole.
6. The skin test sampling kit of claim 1, wherein the film coating kit further comprises a gauge having a hole therein, wherein the hole in the gauge is greater than or equal to the hole in the film coating frame.
7. The skin test sampling pack according to claim 1, wherein in the film coating kit, the film coating patch comprises a transparent film layer, an adhesive layer and a protective layer which are sequentially connected; wherein the protective layer is divided into 2 parts, and the middle part completely covers the composite film.
8. A method of detecting skin conditions, characterized in that the skin detection sampling package according to any one of claims 1-7 is used for sampling, and the observation and analysis are amplified after treatment.
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