CN107764814A - A kind of fecal occult blood test test paper coating process flow - Google Patents
A kind of fecal occult blood test test paper coating process flow Download PDFInfo
- Publication number
- CN107764814A CN107764814A CN201710891227.3A CN201710891227A CN107764814A CN 107764814 A CN107764814 A CN 107764814A CN 201710891227 A CN201710891227 A CN 201710891227A CN 107764814 A CN107764814 A CN 107764814A
- Authority
- CN
- China
- Prior art keywords
- test paper
- coating process
- process flow
- occult blood
- fecal occult
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Abstract
The invention discloses a kind of fecal occult blood test test paper coating process flow, including kit to apply membrane reagent, and the kit, which applies membrane reagent, includes three layers:The formula material of first layer includes：Carboxymethyl cellulose：0.045% 0.055%, SiO：0.045% 0.055%, carmer：4.5% 5.5%, ethylenediamine tetraacetic acid (EDTA)：9.5% 10.5%, peroxidating urine：34.5% 35.5%；The formula material of the second layer includes carboxymethyl cellulose：0.05% 0.15%, SiO：0.045% 0.055%, carmer：4.5% 5.5%, ethylenediamine tetraacetic acid (EDTA)：9.5% 10.5%；The formula material of third layer includes elm and creates wooden fat, the present invention is by using test paper pattern and individually packs, in order to using can directly tear outer packaging, the appropriate test paper of size is taken out directly to be tested, so as to simple to operate, it is easy to use, the features such as quickly learning result, high sensitivity, detect quickly and do not limited by diet and medicine.
Description
Technical field
The present invention relates to Test paper technical field, more particularly to a kind of fecal occult blood test test paper coating process flow.
Background technology
During a small amount of bleeding of alimentary canal, the change without exception of excrement outward appearance, destroy because red blood cell is dissolved, can not find out under microscope
Red blood cell, under this outward appearance and microscope all unverifiable bleeding be referred to as occulting blood, confirm bleeding with chemical method or immunization
Experiment be referred to as occult blood test, generally check that excrement whether in the means with blood, is occulted blood as a kind of most economical actual effect to check
Method, this experiment have important reference value to the early detection of hemorrhage of digestive tract and the screening of tumor in digestive tract, therefore, greatly
It is critically important to preventive usage that feces occult blood, which is examined, in the prior art, the conventional occult blood test of clinic, mainly has chemical method and exempts from
Epidemiology method, chemical method have Ortho-tolidine method, phenolphthalin method, benzidine method, aminopyrine method, guaiaci lignum vinegar method etc., its
Principle makes its discharge nascent oxygen based on the hydrogen peroxide in the iron content ferroheme energy catalytic reagent in hemoglobin, in oxidation
State chromogen substance and develop the color, the depth of colour developing reflect hemoglobin number, that is, the size of amount of bleeding, though chemical method is simple
It is easy, but lack specificity in method because existing chemical method is by other disturbing factors are more, false positive rate is high, glacial acetic acid is made into
Reagent easily forms the limitation of the conditions such as crystal.
Therefore, it is necessary to solve the above problems to invent a kind of fecal occult blood test test paper coating process flow.
The content of the invention
It is an object of the invention to provide a kind of fecal occult blood test test paper coating process flow, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the present invention provides following technical scheme：A kind of fecal occult blood test test paper coating process stream
Journey, including kit apply membrane reagent, and the kit, which applies membrane reagent, includes three layers:
The formula material of first layer includes：Carboxymethyl cellulose：0.045%-0.055%, SiO：0.045%-
0.055%, carmer：4.5%-5.5%, ethylenediamine tetraacetic acid (EDTA)：9.5%-10.5%, peroxidating urine：34.5%-35.5%；
The formula material of the second layer includes carboxymethyl cellulose：0.05%-0.15%, SiO：0.045%-0.055%,
carmer：4.5%-5.5%, ethylenediamine tetraacetic acid (EDTA)：9.5%-10.5%；
The formula material of third layer includes elm and creates wooden fat；
Concrete technology flow process is as follows：
Step 1：Sterilization：Desk by manufacturing shop etc. opens ultraviolet lamp irradiation after disinfecting in alcohol；
Step 2：Reagent preparation solution：According to certain method reagent preparation solution, then rock uniformly；
Step 3：Point sample：Using liquid-transfering gun on blank test paper uniform point sample, 64 every, is placed on after point is good corresponding
Dry position；
Step 4：Spray alcohol：Ethanol is uniformly sprayed to every in the step 3 test paper dried with watering can, is then placed on baking
Dried in case；
Step 5：Point sample：Point sample again, using liquid-transfering gun on blank test paper uniform point sample, 64 every, after point is good
Relevant position is placed on to dry；
Step 6：Cutting：Test paper of the manual cutting test paper into 3.5*3.5cm specifications；
Step 7：Packing：By the test paper being cut into out in step 6 every five it is on chip be a packaging bag, should contain in packaging bag bag
Drier；
Step 8：Sealing：Using sealing machine by the packaging bag enclosing in step 7, and stamp the date of manufacture；
Step 9：Positive control：Positive control is prepared, bag, positive control and test paper are distributed into after stirring
It can not prepare together；
Step 10：Detection：To the test paper that has been produced in step 8 with positive control sampling Detection, according to corresponding test paper
Detection method carries out high temperature detection；
Step 11：Packaging:1 type and 2 types are packaged into respectively to the up-to-standard products by step 10 detection；
Step 12：Storage:The product warehousing of 1 type and 2 types will be packaged as in step 11.
Preferably, the step 1 middle-ultraviolet lamp light irradiation time is arranged to 15-30 minutes.
Preferably, the step 3 and deposition process in step 5 are uniformly quick.
Preferably, the concentration of alcohol sprayed in the step 4 is arranged to 94.5-95.5%.
Preferably, every bag of weight 1.5-2g in the step 9.
Preferably, during the step 10 high temperature detection, detection temperature is arranged to 49-51 degree, and detection time is arranged to 47.5-
48.5 hours.
Preferably, in the step 11,1 type is that inner packing adds two bottles of 100ml pure water, and to be box-packed, 2 types are packed.
The technique effect and advantage of the present invention：The present invention is by using test paper pattern and individually packs, in order to make
With can directly tear outer packaging, take out the appropriate test paper of size and directly tested, so as to have simple to operate, user
Just, the features such as quickly learning result, high sensitivity, detecting quickly and do not limited by diet and medicine.
Embodiment
Below in conjunction with embodiments of the invention, the technical scheme in the embodiment of the present invention is clearly and completely retouched
State, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based on the present invention
In embodiment, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, belongs to the scope of protection of the invention.
Embodiment 1：
A kind of fecal occult blood test test paper coating process flow, including kit apply membrane reagent, the kit film examination
Agent includes three layers:
The formula material of first layer includes：Carboxymethyl cellulose：0.045%, SiO：0.045%, carmer：4.5%,
Ethylenediamine tetraacetic acid (EDTA)：9.5%, peroxidating urine：34.5%；
The formula material of the second layer includes carboxymethyl cellulose：0.05%, SiO：0.045%, carmer：4.5%, two
Amine tetraacethyl：9.5%；
The formula material of third layer includes elm and creates wooden fat；
Concrete technology flow process is as follows：
Step 1：Sterilization：Desk by manufacturing shop etc. is opened ultraviolet lamp after disinfecting in alcohol and irradiated 15 minutes；
Step 2：Reagent preparation solution：According to certain method reagent preparation solution, then rock uniformly；
Step 3：Point sample：Using liquid-transfering gun on blank test paper uniform point sample, 64 every, is placed on after point is good corresponding
Position is dried, and deposition process is uniformly quick；
Step 4：Spray alcohol：It is 94.5% to every in the step 3 uniform spray concentration of the test paper dried with watering can
Ethanol, then put and dry in an oven；
Step 5：Point sample：Point sample again, using liquid-transfering gun on blank test paper uniform point sample, 64 every, after point is good
Relevant position is placed on to dry；
Step 6：Cutting：Test paper of the manual cutting test paper into 3.5*3.5cm specifications；
Step 7：Packing：By the test paper being cut into out in step 6 every five it is on chip be a packaging bag, should contain in packaging bag bag
Drier；
Step 8：Sealing：Using sealing machine by the packaging bag enclosing in step 7, and stamp the date of manufacture；
Step 9：Positive control：Positive control is prepared, bag, positive control and test paper are distributed into after stirring
It can not prepare together, every bag of weight 1.5g；
Step 10：Detection：To the test paper that has been produced in step 8 with positive control sampling Detection, according to corresponding test paper
Detection method carries out high temperature detection, and detection temperature is 49 degree, and detection time is 47.5 hours；
Step 11：Packaging:1 type and 2 types are packaged into respectively to the up-to-standard products by step 10 detection, 1 type is
Inner packing adds two bottles of 100ml pure water, and to be box-packed, 2 types are packed；
Step 12：Storage:The product warehousing of 1 type and 2 types will be packaged as in step 11.
Embodiment 2：
A kind of fecal occult blood test test paper coating process flow, including kit apply membrane reagent, the kit film examination
Agent includes three layers:
The formula material of first layer includes：Carboxymethyl cellulose：0.05%, SiO：0.05%, carmer：5%, diamines
Tetraacethyl：10%, peroxidating urine：35%；
The formula material of the second layer includes carboxymethyl cellulose：0.1%, SiO：0.05%, carmer：5%, diamines tetrem
Acid：10%；
The formula material of third layer includes elm and creates wooden fat；
Concrete technology flow process is as follows：
Step 1：Sterilization：Desk by manufacturing shop etc. opens ultraviolet lamp after disinfecting in alcohol and irradiates 22.5 points
Clock；
Step 2：Reagent preparation solution：According to certain method reagent preparation solution, then rock uniformly；
Step 3：Point sample：Using liquid-transfering gun on blank test paper uniform point sample, 64 every, is placed on after point is good corresponding
Position is dried, and deposition process is uniformly quick；
Step 4：Spray alcohol：With watering can to every in the step 3 uniform spray concentration of the test paper dried be 95% second
Alcohol, then put and dry in an oven；
Step 5：Point sample：Point sample again, using liquid-transfering gun on blank test paper uniform point sample, 64 every, after point is good
Relevant position is placed on to dry；
Step 6：Cutting：Test paper of the manual cutting test paper into 3.5*3.5cm specifications；
Step 7：Packing：By the test paper being cut into out in step 6 every five it is on chip be a packaging bag, should contain in packaging bag bag
Drier；
Step 8：Sealing：Using sealing machine by the packaging bag enclosing in step 7, and stamp the date of manufacture；
Step 9：Positive control：Positive control is prepared, bag, positive control and test paper are distributed into after stirring
It can not prepare together, every bag of weight 1.75g；
Step 10：Detection：To the test paper that has been produced in step 8 with positive control sampling Detection, according to corresponding test paper
Detection method carries out high temperature detection, and detection temperature is 50 degree, and detection time is 48 hours；
Step 11：Packaging:1 type and 2 types are packaged into respectively to the up-to-standard products by step 10 detection, 1 type is
Inner packing adds two bottles of 100ml pure water, and to be box-packed, 2 types are packed；
Step 12：Storage:The product warehousing of 1 type and 2 types will be packaged as in step 11.
Embodiment 3：
A kind of fecal occult blood test test paper coating process flow, including kit apply membrane reagent, the kit film examination
Agent includes three layers:
The formula material of first layer includes：Carboxymethyl cellulose：0.055%, SiO：0.055%, carmer：5.5%,
Ethylenediamine tetraacetic acid (EDTA)：10.5%, peroxidating urine：35.5%；
The formula material of the second layer includes carboxymethyl cellulose：0.15%, SiO：0.055%, carmer：5.5%, two
Amine tetraacethyl：10.5%；
The formula material of third layer includes elm and creates wooden fat；
Concrete technology flow process is as follows：
Step 1：Sterilization：Desk by manufacturing shop etc. is opened ultraviolet lamp after disinfecting in alcohol and irradiated 30 minutes；
Step 2：Reagent preparation solution：According to certain method reagent preparation solution, then rock uniformly；
Step 3：Point sample：Using liquid-transfering gun on blank test paper uniform point sample, 64 every, is placed on after point is good corresponding
Position is dried, and deposition process is uniformly quick；
Step 4：Spray alcohol：It is 95.5% to every in the step 3 uniform spray concentration of the test paper dried with watering can
Ethanol, then put and dry in an oven；
Step 5：Point sample：Point sample again, using liquid-transfering gun on blank test paper uniform point sample, 64 every, after point is good
Relevant position is placed on to dry；
Step 6：Cutting：Test paper of the manual cutting test paper into 3.5*3.5cm specifications；
Step 7：Packing：By the test paper being cut into out in step 6 every five it is on chip be a packaging bag, should contain in packaging bag bag
Drier；
Step 8：Sealing：Using sealing machine by the packaging bag enclosing in step 7, and stamp the date of manufacture；
Step 9：Positive control：Positive control is prepared, bag, positive control and test paper are distributed into after stirring
It can not prepare together, every bag of weight 2g；
Step 10：Detection：To the test paper that has been produced in step 8 with positive control sampling Detection, according to corresponding test paper
Detection method carries out high temperature detection, and detection temperature is 51 degree, and detection time is 48.5 hours；
Step 11：Packaging:1 type and 2 types are packaged into respectively to the up-to-standard products by step 10 detection, 1 type is
Inner packing adds two bottles of 100ml pure water, and to be box-packed, 2 types are packed；
Step 12：Storage:The product warehousing of 1 type and 2 types will be packaged as in step 11.
It can be obtained by embodiment 1-3, the test paper using effect that each item data in embodiment 2 obtains is best, quality highest.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic,
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's
Within protection domain.
Claims (7)
1. a kind of fecal occult blood test test paper coating process flow, including kit apply membrane reagent, it is characterised in that：The reagent
Box, which applies membrane reagent, includes three layers:
The formula material of first layer includes：Carboxymethyl cellulose：0.045%-0.055%, SiO：0.045%-0.055%,
carmer：4.5%-5.5%, ethylenediamine tetraacetic acid (EDTA)：9.5%-10.5%, peroxidating urine：34.5%-35.5%；
The formula material of the second layer includes carboxymethyl cellulose：0.05%-0.15%, SiO：0.045%-0.055%,
carmer：4.5%-5.5%, ethylenediamine tetraacetic acid (EDTA)：9.5%-10.5%；
The formula material of third layer includes elm and creates wooden fat；
Concrete technology flow process is as follows：
Step 1：Sterilization：Desk by manufacturing shop etc. opens ultraviolet lamp irradiation after disinfecting in alcohol；
Step 2：Reagent preparation solution：According to certain method reagent preparation solution, then rock uniformly；
Step 3：Point sample：Using liquid-transfering gun on blank test paper uniform point sample, 64 every, relevant position is placed on after point is good
Dry；
Step 4：Spray alcohol：Ethanol is uniformly sprayed to every in the step 3 test paper dried with watering can, then put in an oven
Drying；
Step 5：Point sample：Point sample again, using liquid-transfering gun on blank test paper uniform point sample, 64 every, is placed on after point is good
Dry relevant position；
Step 6：Cutting：Test paper of the manual cutting test paper into 3.5*3.5cm specifications；
Step 7：Packing：By the test paper being cut into out in step 6 every five it is on chip be a packaging bag, should contain drying in packaging bag bag
Agent；
Step 8：Sealing：Using sealing machine by the packaging bag enclosing in step 7, and stamp the date of manufacture；
Step 9：Positive control：Positive control is prepared, bag is distributed into after stirring, positive control can not with test paper
Prepare together；
Step 10：Detection：Test paper to having been produced in step 8 detects with positive control sampling Detection according to corresponding test paper
Method carries out high temperature detection；
Step 11：Packaging:1 type and 2 types are packaged into respectively to the up-to-standard products by step 10 detection；
Step 12：Storage:The product warehousing of 1 type and 2 types will be packaged as in step 11.
A kind of 2. fecal occult blood test test paper coating process flow according to claim 1, it is characterised in that：The step
One middle-ultraviolet lamp light irradiation time was arranged to 15-30 minutes.
A kind of 3. fecal occult blood test test paper coating process flow according to claim 1, it is characterised in that：The step
Three is uniformly quick with deposition process in step 5.
A kind of 4. fecal occult blood test test paper coating process flow according to claim 1, it is characterised in that：The step
The concentration of alcohol sprayed in four is arranged to 94.5-95.5%.
A kind of 5. fecal occult blood test test paper coating process flow according to claim 1, it is characterised in that：The step
Every bag of weight 1.5-2g in nine.
A kind of 6. fecal occult blood test test paper coating process flow according to claim 1, it is characterised in that：The step
When ten high temperature detect, detection temperature is arranged to 49-51 degree, and detection time is arranged to 47.5-48.5 hours.
A kind of 7. fecal occult blood test test paper coating process flow according to claim 1, it is characterised in that：The step
In 11,1 type is that inner packing adds two bottles of 100ml pure water, and to be box-packed, 2 types are packed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710891227.3A CN107764814A (en) | 2017-09-27 | 2017-09-27 | A kind of fecal occult blood test test paper coating process flow |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710891227.3A CN107764814A (en) | 2017-09-27 | 2017-09-27 | A kind of fecal occult blood test test paper coating process flow |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107764814A true CN107764814A (en) | 2018-03-06 |
Family
ID=61266921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710891227.3A Pending CN107764814A (en) | 2017-09-27 | 2017-09-27 | A kind of fecal occult blood test test paper coating process flow |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110274902A (en) * | 2019-07-23 | 2019-09-24 | 珠海科域生物工程股份有限公司 | A kind of chemical method Test paper of fecal occult blood and preparation method thereof |
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| US5702913A (en) * | 1983-12-21 | 1997-12-30 | Helena Laboratories Corporation | Chromgen-reagent test system |
| WO2000010008A1 (en) * | 1998-08-11 | 2000-02-24 | Diagnostica, Inc. | Self-contained system and method for testing for fecal occult blood |
| CN102331423A (en) * | 2011-09-19 | 2012-01-25 | 苏州浩欧博生物医药有限公司 | Feces occult blood test paper and preparation method thereof |
| CN102778557A (en) * | 2011-04-28 | 2012-11-14 | 美艾利尔（上海）诊断产品有限公司 | Coating liquid for biological detection reagent |
| CN104034725A (en) * | 2014-06-25 | 2014-09-10 | 湖北工业大学 | Rapid detection test paper for trace chromium and preparation method of rapid detection test paper |
| CN106124498A (en) * | 2016-07-12 | 2016-11-16 | 北京珍草中医药技术开发中心 | One is occulted blood detection kit and application thereof |
-
2017
- 2017-09-27 CN CN201710891227.3A patent/CN107764814A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5702913A (en) * | 1983-12-21 | 1997-12-30 | Helena Laboratories Corporation | Chromgen-reagent test system |
| WO2000010008A1 (en) * | 1998-08-11 | 2000-02-24 | Diagnostica, Inc. | Self-contained system and method for testing for fecal occult blood |
| CN102778557A (en) * | 2011-04-28 | 2012-11-14 | 美艾利尔（上海）诊断产品有限公司 | Coating liquid for biological detection reagent |
| CN102331423A (en) * | 2011-09-19 | 2012-01-25 | 苏州浩欧博生物医药有限公司 | Feces occult blood test paper and preparation method thereof |
| CN104034725A (en) * | 2014-06-25 | 2014-09-10 | 湖北工业大学 | Rapid detection test paper for trace chromium and preparation method of rapid detection test paper |
| CN106124498A (en) * | 2016-07-12 | 2016-11-16 | 北京珍草中医药技术开发中心 | One is occulted blood detection kit and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110274902A (en) * | 2019-07-23 | 2019-09-24 | 珠海科域生物工程股份有限公司 | A kind of chemical method Test paper of fecal occult blood and preparation method thereof |
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Application publication date: 20180306 |