CN107759645A - A kind of method that phloridzin is extracted in the slag from Malus toringoides Hughes - Google Patents
A kind of method that phloridzin is extracted in the slag from Malus toringoides Hughes Download PDFInfo
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- CN107759645A CN107759645A CN201710838356.6A CN201710838356A CN107759645A CN 107759645 A CN107759645 A CN 107759645A CN 201710838356 A CN201710838356 A CN 201710838356A CN 107759645 A CN107759645 A CN 107759645A
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- phloridzin
- extracted
- malus toringoides
- ethanol
- dried
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Extraction Or Liquid Replacement (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention discloses a kind of method that phloridzin is extracted in slag from Malus toringoides Hughes, belongs to effective ingredients in plant extraction field, and step is as follows：Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, extraction, filtering is repeated, leaves separation, obtain supernatant；Step 4：Between pH value with KOH regulation supernatants is 45, with the active ingredient phloridzin in macroporous absorbent resin adsorbent solution；Step 5：Eluted, concentrated, dried with ethanol of the volumetric concentration more than 90%.
Description
Technical field
The present invention relates to effective ingredients in plant extraction, the method for extraction phloridzin in specifically a kind of slag from Malus toringoides Hughes.
Background technology
Phloridzin is the glucoside of phloretin.The dihydrochalcone-type material belonged in flavonoids.Phloridzin is from apple
Extract and obtain in fruit, apple bark and leaf etc., it is the aldehydes matter in apple tree.Phloridzin is by phloretin and glycocide
For glucoside bound into glycosides, its catabolite phloroglucin, can effectively suppress effect of microbial activities etc..It is both domestic and external to face
Bed research shows that phloridzin has the effect of fine in terms of diabetes are treated.
What be presently, there are extracts phloridzin method in apple, but it has been investigated that Malus toringoides Hughes are given birth in high altitude localities
It is long, in vivo there is phloridzin, it is necessary to take reasonable manner to be extracted.
The content of the invention
It is an object of the invention to：For above-mentioned problem, there is provided one kind extracts phloridzin from Malus toringoides Hughes slag
Method, step is as follows：
Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；
Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；
Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, extraction, filtering is repeated, leaves separation, obtain
Clear liquid；
Step 4：Between pH value with KOH regulation supernatants is 4-5, with the active ingredient in macroporous absorbent resin adsorbent solution
Phloridzin；
Step 5：Eluted, concentrated, dried with ethanol of the volumetric concentration more than 90%.
As an improvement, the ethanol of described step five is absolute ethyl alcohol.
As an improvement, the ethanol of described step two and the volume ratio of chloroform are 5:1.
As an improvement, the pH value of described step four kind KOH regulation supernatants is 3.
As an improvement, the solid-liquid ratio of the dried fibrous residue of described step three kind and ethanol chloroformic solution is 1:10-1:
50。
As an improvement, described KOH concentration is 2%-5%.
As an improvement, the Extracting temperature of step 3 kind is 60 DEG C -80 DEG C
As an improvement, extraction time is 50min-200min.
The present invention, can be effectively from Malus toringoides Hughes using the concentration and purifying technique of rational solid-liquid ratio and ethanol
Extract phloridzin.
Using recovery rate=phloridzin finished weight/dried Malus toringoides Hughes fibrous residue
Recovery rate is overall between 0.1-0.2, wherein under preferably process conditions, recovery rate is usually 0.15.
Embodiment
The present invention is described in detail below.
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Specific embodiment 1：
Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；
Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；
Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, solid-liquid ratio 1:The volume of 10 ethanol and chloroform
Than for 5:1, Extracting temperature is 60 DEG C -80 DEG C, and extraction time is that 100min repeats extraction, filtering, leaves separation, obtains supernatant
Liquid；
Step 4：Between pH value with KOH regulation supernatants is 4, KOH concentration is 2%, with macroporous absorbent resin adsorbent solution
Active ingredient phloridzin；
Step 5：Eluted, concentrated, dried with ethanol of the volumetric concentration more than 90%.
The recovery rate of the present embodiment is 0.12.
Specific embodiment 2：
Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；
Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；
Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, solid-liquid ratio 1:The volume of 50 ethanol and chloroform
Than for 5:1, Extracting temperature is 60 DEG C -80 DEG C, and extraction time is that 200min repeats extraction, filtering, leaves separation, obtains supernatant
Liquid；
Step 4：Between pH value with KOH regulation supernatants is 5, KOH concentration is 5%, with macroporous absorbent resin adsorbent solution
Active ingredient phloridzin；
Step 5：Eluted, concentrated with absolute ethyl alcohol, being dried.
The recovery rate of the present embodiment is 0.14.
Specific embodiment 3：
Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；
Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；
Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, solid-liquid ratio 1:The volume of 20 ethanol and chloroform
Than for 5:1, Extracting temperature is 60 DEG C -80 DEG C, and extraction time is that 50min-200min repeats extraction, filtering, leaves separation, obtains
Supernatant；
Step 4：Between pH value with KOH regulation supernatants is 5, KOH concentration is 3%, with macroporous absorbent resin adsorbent solution
Active ingredient phloridzin；
Step 5：Eluted, concentrated, dried with ethanol of the volumetric concentration more than 90%.
The recovery rate of the present embodiment is 0.18.
Specific embodiment 4：
Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；
Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；
Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, solid-liquid ratio 1:The volume of 10 ethanol and chloroform
Than for 5:1, Extracting temperature is 60 DEG C -80 DEG C, and extraction time is that 50min repeats extraction, filtering, leaves separation, obtains supernatant；
Step 4：Between pH value with KOH regulation supernatants is 5, KOH concentration is 5%, with macroporous absorbent resin adsorbent solution
Active ingredient phloridzin；
Step 5：Eluted, concentrated with volumetric concentration absolute ethyl alcohol, being dried.
The recovery rate of the present embodiment is 0.19.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
All any modification, equivalent and improvement made within principle etc., should be included in the scope of the protection.
Claims (8)
1. the method for phloridzin is extracted in a kind of slag from Malus toringoides Hughes, it is characterised in that step is as follows：
Step 1：Malus toringoides Hughes fibrous residue after squeezing the juice is dried step；
Step 2：Dried fibrous residue is dropped into room temperature and is crushed to complete sieving repeatedly；
Step 3：Fibrous residue after crushing and ethanol and chloroformic solution are mixed, extraction, filtering is repeated, leaves separation, obtain
Clear liquid；
Step 4：Between pH value with KOH regulation supernatants is 4-5, with the active ingredient in macroporous absorbent resin adsorbent solution
Phloridzin；
Step 5：Eluted, concentrated, dried with ethanol of the volumetric concentration more than 90%.
2. the method for phloridzin is extracted in the slag according to claim 1 from Malus toringoides Hughes, it is characterised in that described step
Five ethanol is absolute ethyl alcohol.
3. the method for phloridzin is extracted in the slag according to claim 2 from Malus toringoides Hughes, it is characterised in that described step
Two ethanol and the volume ratio of chloroform are 5:1.
4. the method for phloridzin is extracted in the slag according to claim 3 from Malus toringoides Hughes, it is characterised in that described step
The pH value of four kinds of KOH regulation supernatants is 3.
5. the method for phloridzin is extracted in the slag according to claim 4 from Malus toringoides Hughes, it is characterised in that described step
The solid-liquid ratio of three kinds of dried fibrous residue and ethanol chloroformic solutions is 1:10-1:50.
6. the method for phloridzin is extracted in the slag according to claim 5 from Malus toringoides Hughes, it is characterised in that described KOH
Concentration is 2%-5%.
7. the method for phloridzin is extracted in the slag according to claim 6 from Malus toringoides Hughes, it is characterised in that step 3 kind
Extracting temperature is 60 DEG C -80 DEG C.
8. the method for phloridzin is extracted in the slag according to claim 7 from Malus toringoides Hughes, it is characterised in that extraction time is
50min-200min。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710838356.6A CN107759645A (en) | 2017-09-18 | 2017-09-18 | A kind of method that phloridzin is extracted in the slag from Malus toringoides Hughes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710838356.6A CN107759645A (en) | 2017-09-18 | 2017-09-18 | A kind of method that phloridzin is extracted in the slag from Malus toringoides Hughes |
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| CN107759645A true CN107759645A (en) | 2018-03-06 |
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| CN201710838356.6A Pending CN107759645A (en) | 2017-09-18 | 2017-09-18 | A kind of method that phloridzin is extracted in the slag from Malus toringoides Hughes |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1309539A1 (en) * | 1985-04-18 | 1996-04-20 | Плодоовощной институт им.И.В.Мичурина | Process for recovering phlorizin from bark of apple tree sprouts |
| CN1683382A (en) * | 2005-03-11 | 2005-10-19 | 天津市尖峰天然产物研究开发有限公司 | Process for extracting phlorhizin from bark, root, branch and leaf of rosaceous plant and juice pressing waste material |
| CN103214530A (en) * | 2013-03-29 | 2013-07-24 | 陕西工业职业技术学院 | Separation and purification method for phlorizin in apple pomace |
| CN103864864A (en) * | 2014-03-27 | 2014-06-18 | 江苏斯威森生物医药工程研究中心有限公司 | Method for efficiently extracting phlorizin from plants |
| CN104098623A (en) * | 2014-07-21 | 2014-10-15 | 神农架聚能药业有限公司 | Method for extracting phloridzin of malus hupehensis (pamp.) rehd |
-
2017
- 2017-09-18 CN CN201710838356.6A patent/CN107759645A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1309539A1 (en) * | 1985-04-18 | 1996-04-20 | Плодоовощной институт им.И.В.Мичурина | Process for recovering phlorizin from bark of apple tree sprouts |
| CN1683382A (en) * | 2005-03-11 | 2005-10-19 | 天津市尖峰天然产物研究开发有限公司 | Process for extracting phlorhizin from bark, root, branch and leaf of rosaceous plant and juice pressing waste material |
| CN103214530A (en) * | 2013-03-29 | 2013-07-24 | 陕西工业职业技术学院 | Separation and purification method for phlorizin in apple pomace |
| CN103864864A (en) * | 2014-03-27 | 2014-06-18 | 江苏斯威森生物医药工程研究中心有限公司 | Method for efficiently extracting phlorizin from plants |
| CN104098623A (en) * | 2014-07-21 | 2014-10-15 | 神农架聚能药业有限公司 | Method for extracting phloridzin of malus hupehensis (pamp.) rehd |
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Application publication date: 20180306 |