CN107759117A - A kind of method using false bacillus firmus DSM8715 intensifying regenerating aggregates - Google Patents
A kind of method using false bacillus firmus DSM8715 intensifying regenerating aggregates Download PDFInfo
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- CN107759117A CN107759117A CN201711057551.1A CN201711057551A CN107759117A CN 107759117 A CN107759117 A CN 107759117A CN 201711057551 A CN201711057551 A CN 201711057551A CN 107759117 A CN107759117 A CN 107759117A
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- dsm8715
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B20/00—Use of materials as fillers for mortars, concrete or artificial stone according to more than one of groups C04B14/00 - C04B18/00 and characterised by shape or grain distribution; Treatment of materials according to more than one of the groups C04B14/00 - C04B18/00 specially adapted to enhance their filling properties in mortars, concrete or artificial stone; Expanding or defibrillating materials
- C04B20/02—Treatment
- C04B20/023—Chemical treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/91—Use of waste materials as fillers for mortars or concrete
Abstract
The invention provides a kind of method using false bacillus firmus DSM8715 intensifying regenerating aggregates：1) false bacillus firmus DSM8715 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid includes the 10~45g/L propane sulfonic acid of 3 Cyclohexylamino 1,3~15g/L sodium lactate and 3~10g/L calcium hydroxide；2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.For the regeneration aggregate being prepared using the method for the invention compared with before microbiological treatment, water absorption rate reduces 24.4~31.7%；Crush index value reduces 19.78%.The regeneration mortar compression strength increase by 15.8~21.1% prepared by the intensifying regenerating aggregate.
Description
Technical field
The invention belongs to technical field of concrete, more particularly to one kind to be strengthened using false bacillus firmus DSM8715
The method of regeneration aggregate.
Background technology
With urbanization progress faster in world wide, construction industry enters the high speed development stage.A large amount of old buildings are split
Remove, generate substantial amounts of building waste, wherein discarded concrete portion is maximum.Data shows, the whole world from 1991~
Between 10 years in 2000, discarded concrete (including from the underproof product of armored concrete factory) total amount is more than 1,000,000,000 tons.I
40,000,000 tons of calculating of building waste are removed by annual by state, wherein 34% is concrete block, then resulting discarded concrete is just
There are 13,600,000 tons, and with the quickening of economic construction paces, increased trend is presented.The discarded concrete of such flood tide is except processing
Expense is surprising outer, it is also necessary to takes substantial amounts of vacant lot storage, pollutes environment, waste arable land, turn into a big public hazards in city.
Discarded concrete is not only high-quality concrete aggregate, makees to gather materials with many advantages with waste concrete block, such as
After building disintegrates, concrete block and flour sand after high-quality crushing and screening can be as the regeneration of concrete, therefore utilize discarded
Building rubbish produces regeneration aggregate and prepares the important development direction that regeneration concrete is construction industry from now on.Discarded concrete
The processing method of most worthy is exactly that it is re-used production regeneration aggregate as renewable resource, is turned waste into wealth.
Regeneration aggregate is applied with before good economic benefit, social benefit, environmental benefit and the application of good market
Scape.In the prior art, or ball mill activating and regenerating aggregate is used so that the quality of regeneration aggregate greatly improves, available for producing
Reinforced concrete member；Or using the glacial acetic acid of 5% concentration and the hydrochloric acid solution of 3% concentration to regeneration aggregate processing；Or use
Cement and Separate Fine-grained Minerals slurry liquid (such as flyash, silica flour, siliceous waterproofing agent or calcium sulphoaluminate class swelling agent) are to regeneration aggregate
The processing such as immersion, dry.But because regeneration aggregate produces hole, crack etc. during broken or processing, regeneration aggregate is caused to inhale
Water rate is larger, causes regeneration concrete hydraulic performance decline, constrains the development in regeneration aggregate production regeneration concrete field.
The content of the invention
In view of this, in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to which water absorption rate and pressure can be reduced by providing one kind
Broken index, improve the method using false bacillus firmus DSM8715 intensifying regenerating aggregates of mixed mortar compression strength.
The invention provides a kind of method using false bacillus firmus DSM8715 intensifying regenerating aggregates, including it is as follows
Step：
1) false bacillus firmus DSM8715 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture
The preparation raw material of liquid includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3~15g/L sodium lactate and 3~10g/L hydrogen
Calcium oxide；
2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.
Preferably, the pH value of the Mineralized Culture liquid is 10.1~10.9.
Preferably, in the mixed-culture medium that the step 1) obtains, false bacillus firmus DSM8715 bacterium number for 1 ×
107~109cfu/L。
Preferably, step 1) the false bacillus firmus DSM8715 is obtained through microbiological culture media culture；
The microbiological culture media includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions；The beef
Cream culture medium is using water as solvent, including 2.5~4.5g/L beef extract and 8~15g/L peptone；The 3- Cyclohexylaminos-
1- propane sulfonic acid buffer solution includes 120~170g/L 3- Cyclohexylamino -1- propane sulfonic acid using water as solvent；The 3- Cyclohexylaminos-
The pH value of 1- propane sulfonic acid buffer solutions is 9~10.5；The body of the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions
Product ratio is (70~95):(10~20).
Preferably, the culture is carried out under oscillating condition, and the frequency of the vibration is 120~180rpm；The culture
Temperature be 25~35 DEG C, the time of the culture is 8~48h.
Preferably, after microbiological culture media culture, centrifugation obtains thalline and sunk the false bacillus firmus DSM8715
Form sediment；The bacterial sediment is washed with the Mineralized Culture liquid 2~3 times；The rotating speed of the centrifugation is 5000~7000rpm, described
The time of centrifugation is 8~12 minutes.
Preferably, the mode of the step 2) spray is intermittent shower, 0.5~1.5h is sprayed every 10~12h, per 1kg
The amount of regeneration aggregate spray mixed-culture medium is 1~3mL/min.
Preferably, the total time of the spray is 15~30d.
Further, step 2 is replaced with as optional technical scheme, the step 2) ')：By regeneration aggregate described
Soaked in the mixed-culture medium that step 1) obtains, then spray the Regenerated Bone after the immersion with Mineralized Culture liquid step 1) described
Material, strengthened regeneration aggregate.
Preferably, step 2 ') time of the immersion is 1~5h；Step 2 ') total time of the spray is 15~30d.
Beneficial effect：
The invention provides a kind of method using false bacillus firmus DSM8715 intensifying regenerating aggregates, including it is as follows
Step：1) false bacillus firmus DSM8715 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid
Preparation raw material include 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3~15g/L sodium lactate and 3~10g/L hydrogen-oxygen
Change calcium；2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.Regeneration aggregate is blended
After the spray of nutrient solution or immersion, microbes are attached in regeneration aggregate surface pore, using the composition in Mineralized Culture liquid,
By being metabolized calcium ion Ca therein2+It is converted into calcium carbonate CaCO3It is deposited in regeneration aggregate surface pore, so as to block again
Raw aggregate surface pore, reduce the water absorption rate of regeneration aggregate.Using the regeneration aggregate that the method for the invention is prepared with it is micro-
Compared before biological treatment, water absorption rate reduces 24.4~31.7%；Crush index value reduces 19.78%.By the reinforcing again
Regeneration mortar compression strength increase by 15.8~21.1% prepared by raw aggregate.
Embodiment
The invention provides a kind of method of microbial augmentation regeneration aggregate, comprise the following steps：
1) false bacillus firmus DSM8715 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture
The preparation raw material of liquid includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3~15g/L sodium lactate and 3~10g/L hydrogen
Calcium oxide；
2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.
The present invention is not special to the source of the false bacillus firmus DSM8715 (Bacilluspseudofirmus)
Limit.In the present invention, the false bacillus firmus DSM8715 is tested by TU Delft Polytechnics Microlab
Room Henk Jonkers gift acquisition.
In the present invention, the false bacillus firmus DSM8715 preferably obtains through microbiological culture media culture；It is described micro-
Biological medium preferably includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid (CAPS) buffer solution.Beef extract culture medium
Nutritional ingredient is provided for the activation of strain, and CAPS buffer solutions are keeping the pH of microbiological culture media stable.
In the present invention, using water as solvent, the beef extract culture medium preferably includes 2.5~4.5g/L beef extract and 8
The beef extract of~15g/L peptone, more preferably 3.0~4.0g/L and 10~13g/L peptone, most preferably 3.53g/
L beef extract and 11.76g/L peptone.
In the present invention, used after the preferred sterilizing of the beef extract culture medium；The sterilizing is preferably high-temperature sterilization, described
The temperature of sterilizing is preferably 121 DEG C, and the time of the sterilizing is preferably 15min.The present invention is to the equipment of the sterilizing without spy
It is different to require, using those skilled in the art's conventional sterilant equipment, such as high-pressure steam sterilizing pan.
In the present invention, using water as solvent, the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solutions preferably include 120~170g/
L 3- Cyclohexylamino -1- propane sulfonic acid, most preferably more preferably 140~160g/L, 147.5g/L.
In the present invention, the pH value of the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solutions is preferably 9~10.5, more preferably
9.5~10.The method that the present invention adjusts pH value to 3- Cyclohexylamino -1- propane sulfonic acid buffer solution is not particularly limited, using ability
The Normal practice of field technique personnel.In the specific embodiment of the invention, preferably 3- Cyclohexylaminos -1- third is adjusted with NaOH
The pH value of sulfonate buffer.The NaOH is preferably the NaOH aqueous solution, and the concentration of the NaOH aqueous solution is preferably 1~10mol/
L, more preferably 5~7mol/l.
In the present invention, used after the preferred sterilizing of the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solution；The sterilizing is preferably
High-temperature sterilization, the temperature of the sterilizing is preferably 121 DEG C, and the time of the sterilizing is preferably 15min.The present invention is to the sterilizing
Equipment there is no particular/special requirement, using those skilled in the art's conventional sterilant equipment, such as high-pressure steam sterilizing pan.
In the present invention, the volume ratio of the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions is preferably
(70~95):(10~20), more preferably (80~90):(12~18), most preferably 85:15.The present invention is by beef extract culture
Base and 3- Cyclohexylamino -1- propane sulfonic acid buffer solution aseptically mix according to above-mentioned volume ratio, obtain pH value as 9.5~10
Microbiological culture media.
False bacillus firmus DSM8715 is inoculated in microbiological culture media by the present invention to be cultivated.The present invention is to the vacation
Bacillus firmus DSM8715 inoculum concentration does not have particular/special requirement, specially with oese according to the conventional behaviour of the art
Make a little colony inoculation of picking in microbiological culture media.
In the present invention, the culture is preferably carried out under oscillating condition, and the frequency of the vibration is preferably 120~
180rpm, more preferably 130~160rpm, most preferably 150rpm.In the present invention, the temperature of the culture be preferably 25~
35 DEG C, more preferably 28~32 DEG C, most preferably 29~31 DEG C.In the present invention, the time of the culture is preferably 8~48h,
More preferably 10~24h, most preferably 12~20h.The present invention is not particularly limited to the instrument of the shaken cultivation, using this
The equipment that art personnel routinely select, such as constant-temperature table.
In the present invention, for the false bacillus firmus DSM8715 after microbiological culture media culture, centrifugation obtains thalline
Precipitation；In the present invention, the rotating speed of the centrifugation is preferably 5000~7000rpm, more preferably 6000rpm；The centrifugation
Time is 8~12min, more preferably 10min.After centrifugation, the bacterial sediment is washed 2~3 times with the Mineralized Culture liquid.This
The purpose that invention is precipitated using Mineralized Culture liquid washing thalline is the further beef extract and albumen removed in microbiological culture media
Peptone composition, during avoiding follow-up Mineralized Culture, the composition such as beef extract, peptone is attached to regeneration aggregate surface, influences
Reinforcing of the microorganism to regeneration aggregate.
The present invention mixes false bacillus firmus DSM8715 with Mineralized Culture liquid, obtains mixed-culture medium.In the present invention
In, the Mineralized Culture liquid preferably includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3~15g/L using water as solvent
3- Cyclohexylamino -1- the propane sulfonic acid of the calcium hydroxide of sodium lactate and 3~10g/L, more preferably 15~35g/L, 5~10g/L
3- Cyclohexylamino -1- propane sulfonic acid, the 6g/L sodium lactate of the calcium hydroxide of sodium lactate and 4~8g/L, most preferably 22.13g/L
With 6.67g/L calcium hydroxide.In the present invention, the sodium lactate provides required carbon source for bacterial growth, to form carbonic acid
Calcium provides carbon source；The calcium hydroxide exists in the form of suspension, and calcium source is provided to form calcium carbonate；The 3- hexamethylenes ammonia
Base -1- propane sulfonic acid maintains the pH stable of the Mineralized Culture liquid as composition is buffered.In the present invention, bacterium is with sodium lactate
For nutrition, by aerobic respiration or other metabolism by sodium lactate be converted into carbon dioxide/carbonate be discharged into it is extracellular, with calcium
Ions binding generates calcium carbonate.
In the present invention, the Mineralized Culture liquid is preferably prepared using two-step method.First making lactic acid sodium and hydroxide
The mixed solution (A liquid) of calcium, then the A liquid is mixed with the aqueous solution (B liquid) of 3- Cyclohexylamino -1- propane sulfonic acid, obtain
Mineralized Culture liquid.The pH value of A liquid or B liquid of the present invention is preferably 10.1~10.9, and more preferably 10.4~10.6, most preferably
For 10.5.The present invention individually adjusts the pH value of A liquid and B liquid in process for preparation；The present invention preferably adjusts A liquid with acetum
PH value, the mass concentration of acetic acid is preferably 10~100% in the acetum, more preferably 50~99%, be most preferably
80~95%；The present invention preferably adjusts the pH of B liquid with NaOH, and the concentration of the NaOH solution is preferably 1~10mol/L, more excellent
Elect 5~7mol/L as.After A liquid and B liquid are mixed, Mineralized Culture liquid is obtained；The pH value of the Mineralized Culture liquid is preferably 10.1
~10.9, more preferably 10.4~10.6, most preferably 10.5.It is in order to more that the present invention prepares Mineralized Culture liquid using two-step method
Good regulation pH value.
The present invention mixes false bacillus firmus DSM8715 with Mineralized Culture liquid, obtains mixed-culture medium.Described mixed
Close in nutrient solution, false bacillus firmus DSM8715 bacterium number is preferably 1 × 107~109Cfu/L, more preferably 1 ×
108cfu/L。
The present invention makes false bacillus firmus DSM8715 be attached to Regenerated Bone using mixed-culture medium spray regeneration aggregate
In the surface pore of material, while microbes are by being metabolized the calcium ion Ca in nutrient solution2+It is converted into calcium carbonate CaCO3Deposition
In regeneration aggregate surface pore, so as to block regeneration aggregate surface pore, the water absorption rate of regeneration aggregate is reduced.In the present invention
In, the granularity of the regeneration aggregate is preferably 0.10~25mm.In an embodiment of the present invention, the granularity of regeneration aggregate is 0.15
~4.75mm or 5~10mm.
The present invention does not have special limitation to the source of the regeneration aggregate, using technology well known to those skilled in the art
Scheme is prepared by discarded concrete.Specifically, in an embodiment of the present invention, the preparation method of the regeneration aggregate is preferred
Comprise the following steps：
Discarded concrete is crushed, the reinforcing bar in the concrete after crushing is taken out, obtains mass concrete；By the nothing
Armored concrete crushes again, obtains 0.10~25mm regeneration aggregate.
In the present invention, the broken degree of the mass concrete is for 5~20mm or less than 4.75mm；It is described discarded mixed
The broken mode of solidifying soil is preferably to be broken off reinforcing bar or to take out reinforcing bar after hand breaking, obtain no-reinforcing-bar using quartering hammer
Concrete.The present invention is not particularly limited to the mode that mass concrete crushes again, using the conventional method in this area
.
The present invention is using on mixed-culture medium spray to regeneration aggregate, and strengthened regeneration aggregate.In the present invention, it is described
Spray is to keep the surface wettability of regeneration aggregate as standard, it is preferred to use the mode of intermittent shower, 0.5 is sprayed every 10~12h
~1.5h, the amount per 1kg regeneration aggregates spray mixed-culture medium is 1~3mL/min；It is furthermore preferred that 1h is sprayed every 11h, often
The amount of 1kg regeneration aggregates spray mixed-culture medium is 2mL/min.During spray, keep the temperature of Mineralized Culture liquid for 25~
35 DEG C, more preferably 28~32 DEG C, most preferably 29~31 DEG C.
The total time of the spray is 15~30d, more preferably 18~25d, most preferably 20d.Wherein, spray it is total when
Between include spray time and spray off time.In the present invention, the purpose for spraying mixed-culture medium is to keep regeneration aggregate
Moistening, provide moisture and nutriment for the vital movement of microorganism.Meanwhile microorganism is by being metabolized the calcium in nutrient solution
Ion Ca2+It is converted into calcium carbonate CaCO3It is deposited in regeneration aggregate surface pore, so as to block regeneration aggregate surface pore, drop
The water absorption rate of low regeneration aggregate.In the present invention, the Mineralized Culture liquid during spray act as provide calcium source (calcium from
Son) and the suitable living environment of microorganism and higher mineralization activity.
As optional technical scheme, step 2 ') in, the soak time is 1~5h, more preferably 1.5~3h, optimal
Elect 2h as.Regeneration aggregate is soaked in mixed-culture medium, and microbes are attached in regeneration aggregate surface pore, while microorganism
Can be by being metabolized the calcium ion Ca in nutrient solution2+It is converted into calcium carbonate CaCO3It is deposited in regeneration aggregate surface pore, so as to
Regeneration aggregate surface pore is blocked, reduces the water absorption rate of regeneration aggregate.After immersion terminates, separation of solid and liquid obtains carrying strain
Regeneration aggregate.The present invention is by Mineralized Culture liquid spray to the regeneration aggregate for carrying strain, and strengthened regeneration
Aggregate.In the present invention, the spray is to keep the surface wettability of regeneration aggregate as standard, it is preferred to use the side of intermittent shower
Formula, 0.5~1.5h is sprayed every 10~12h, the amount per 1kg regeneration aggregates spray mixed-culture medium is 1~3mL/min；It is more excellent
Choosing, 1h is sprayed every 11h, the amount per 1kg regeneration aggregates spray mixed-culture medium is 2mL/min.During spray, ore deposit is kept
The temperature for changing nutrient solution is 25~35 DEG C, more preferably 28~32 DEG C, most preferably 29~31 DEG C.The total time of the spray is
15~30d, more preferably 18~25d, most preferably 20d.Wherein, when including spray time and spray gap the total time of spray
Between.
In the present invention, the purpose for spraying Mineralized Culture liquid is the moistening for keeping regeneration aggregate, is the life of microorganism
Activity provides moisture and nutriment.Meanwhile microorganism is by being metabolized the calcium ion Ca in nutrient solution2+It is converted into calcium carbonate
CaCO3It is deposited in regeneration aggregate surface pore, so as to block regeneration aggregate surface pore, reduces the water absorption rate of regeneration aggregate.
In the present invention, the Mineralized Culture liquid acting as in immersion process provides calcium source (calcium ion) and the suitable life of microorganism
Dis environment and higher mineralization activity.
After the regeneration aggregate that strengthened, preferred pair regeneration aggregate is dried the present invention.The present invention is to the drying
Method is not particularly limited, the technical scheme dried using aggregate well known to those skilled in the art；In the reality of the present invention
Apply in example, can be specifically dried using air dry oven or naturally dry.When the drying is preferably forced air drying, institute
State to dry and be preferably specially：Dried under the conditions of 25~35 DEG C to constant weight.
In the present invention, according to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》At test microbes
The water absorption rate change of intensifying regenerating aggregate before and after reason.
A kind of microbial augmentation regeneration aggregate provided by the invention and preparation method thereof is carried out with reference to embodiment detailed
Thin explanation, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, reinforcing bar is broken off to come, hardly possible is taken out
Reinforcing bar by being taken out after hand breaking, the mass concrete tentatively crushed, then crushed with jaw crusher, sieve
Go out 5~10mm aggregate.
The preparation of Mineralized Culture liquid：Calcium hydroxide 6.67g is weighed, sodium lactate 6g, measures deionized water 830mL, it is fully mixed
It is even, 850mL is settled to, obtains A liquid；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionized water 130mL, it is fully mixed
PH value is adjusted to 10.5 with 6mol/L sodium hydroxides and 90% acetum after even, is settled to 150mL, is obtained B liquid；By A liquid
It is well mixed with B liquid, as Mineralized Culture liquid.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionization
Water 130mL, pH value is adjusted to 10 with 6mol/L sodium hydroxides after fully mixing, 150mL is settled to, prepares 3- Cyclohexylaminos -1-
Propane sulfonic acid buffer solution, 121 DEG C of sterilizing 15min, is cooled to room temperature；It will be cooled to the beef extract culture medium and 3- hexamethylene ammonia of room temperature
Base -1- propane sulfonic acid buffer solution mixes, and obtains microbiological culture media.
False bacillus firmus DSM8715 is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C,
The frequency of vibration is 150rpm, and culture is centrifuged after terminating and washed 3 times with Mineralized Culture liquid, obtains bacterial precipitation, bacterium is sunk
Shallow lake is added in Mineralized Culture liquid, obtains mixed-culture medium, and it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；In normal temperature
Under the conditions of mixed-culture medium is sprayed to regeneration aggregate, 1h is sprayed every 11h, per 1kg regeneration aggregates spray mixed-culture medium
Measure as 2mL/min, intermittent shower 20 days, strengthened regeneration aggregate.According to standard GB/T25176-2010《Concrete and mortar
Use regeneration aggregate》The test microbes performance such as water absorption rate of intensifying regenerating aggregate before and after the processing, the results are shown in Table 1：
Intensifying regenerating aggregate performance comparison before and after the processing in the embodiment of the present invention 1 of table 1
As can be seen from Table 1, water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 6.5%, the water suction of before processing
Rate is 8.6%, and compared with before microbiological treatment, water absorption rate reduces 24.4%.After microbial augmentation, crushing refers to regeneration aggregate
Mark reduces 19.78%.
Embodiment 2
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, reinforcing bar is broken off to come, hardly possible is taken out
Reinforcing bar by being taken out after hand breaking, the mass concrete tentatively crushed, then crushed with jaw crusher, sieve
Go out to be less than 4.75mm aggregate, finally prepare 0.15~4.75mm aggregate.
The preparation of Mineralized Culture liquid：Calcium hydroxide 6.67g is weighed, sodium lactate 6g, measures deionized water 830mL, it is fully mixed
It is even, 850mL is settled to, obtains A liquid；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionized water 130mL, it is fully mixed
PH value is adjusted to 10.5 with 6mol/L sodium hydroxides and 90% acetum after even, is settled to 150mL, is obtained B liquid；By A liquid
It is well mixed with B liquid, as Mineralized Culture liquid.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionization
Water 130mL, pH value is adjusted to 10 with 6mol/L sodium hydroxides after fully mixing, 150mL is settled to, prepares 3- Cyclohexylaminos -1-
Propane sulfonic acid buffer solution, 121 DEG C of sterilizing 15min, is cooled to room temperature；It will be cooled to the beef extract culture medium and 3- hexamethylene ammonia of room temperature
Base -1- propane sulfonic acid buffer solution mixes, and obtains microbiological culture media.
False bacillus firmus DSM8715 is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C,
The frequency of vibration is 150rpm, and culture is centrifuged after terminating and washed 3 times with Mineralized Culture liquid, obtains bacterial precipitation, bacterium is sunk
Shallow lake is added in Mineralized Culture liquid, obtains mixed-culture medium, and it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；In normal temperature
Under the conditions of mixed-culture medium is sprayed to regeneration aggregate, 1h is sprayed every 11h, per 1kg regeneration aggregates spray mixed-culture medium
Measure as 2mL/min, intermittent shower 20 days, strengthened regeneration aggregate.According to standard GB/T25176-2010《Concrete and mortar
Use regeneration aggregate》The test microbes performance such as water absorption rate of intensifying regenerating aggregate before and after the processing, the results are shown in Table 2~3：
Table 2：The granularity of intensifying regenerating aggregate in the embodiment of the present invention 2
Intensifying regenerating aggregate is through the performance before and after microbiological treatment in the embodiment of the present invention 2 of table 3
It can be drawn by table 3, the water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 4.1%, the suction of before processing
Water rate is 6.0%, and compared with before microbiological treatment, water absorption rate reduces 31.7%.
Embodiment 3
Regeneration mortar is prepared as reclaimed sand by the use of untreated regeneration aggregate in embodiment 2 and the regeneration aggregate after reinforcing,
Proportioning is shown in Table 4, investigates influence of the regeneration aggregate after microbial augmentation to mortar compression strength, the results are shown in Table 5.
Table 4 regenerates mortar mix ratio
Table 5 regenerates mortar compression strength
It can be drawn by table 5, after microbiological treatment, the compression strength for regenerating mortar adds 15.8~21.1%.
Embodiment 4
Regeneration mortar is prepared as reclaimed sand by the use of untreated regeneration aggregate in embodiment 2 and the regeneration aggregate after reinforcing,
Proportioning is shown in Table 6, investigates influence of the regeneration aggregate after microbial augmentation to mortar compression strength, the results are shown in Table 7.
Table 6 regenerates mortar mix ratio
Table 7 regenerates mortar compression strength
Embodiment 5
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, reinforcing bar is broken off to come, hardly possible is taken out
Reinforcing bar by being taken out after hand breaking, the mass concrete tentatively crushed, then crushed with jaw crusher, sieve
Go out 5~10mm aggregate.
The preparation of Mineralized Culture liquid：Calcium hydroxide 6.67g is weighed, sodium lactate 6g, measures deionized water 830mL, it is fully mixed
It is even, 850mL is settled to, obtains A liquid；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionized water 130mL, it is fully mixed
PH value is adjusted to 10.5 with 6mol/L sodium hydroxides and 90% acetum after even, is settled to 150mL, is obtained B liquid；By A liquid
It is well mixed with B liquid, as Mineralized Culture liquid.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionization
Water 130mL, pH value is adjusted to 10 with 6mol/L sodium hydroxides or 90% acetum after fully mixing, 150mL is settled to, matches somebody with somebody
3- Cyclohexylaminos -1- propane sulfonic acid buffer solutions processed, 121 DEG C of sterilizing 15min, are cooled to room temperature；It will be cooled to the beef extract training of room temperature
Base and the mixing of 3- Cyclohexylamino -1- propane sulfonic acid buffer solution are supported, obtains microbiological culture media.
False bacillus firmus DSM8715 is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C,
The frequency of vibration is 150rpm, and culture is centrifuged after terminating and washed 3 times with Mineralized Culture liquid, obtains bacterial precipitation, bacterium is sunk
Shallow lake is added in Mineralized Culture liquid, obtains mixed-culture medium, and it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；Will regeneration
Aggregate, which is added in mixed-culture medium, soaks 2h, separation of solid and liquid, obtains carrying the regeneration aggregate of strain.Then by Mineralized Culture
Liquid intermittent shower sprays 1h on the regeneration aggregate for carrying strain every 11h, per the spray mixing training of 1kg regeneration aggregates
The amount of nutrient solution is 1~3mL/min.After intermittent shower 20d, strengthened regeneration aggregate.According to standard GB/T25176-2010《It is mixed
Solidifying soil and mortar regeneration aggregate》The test microbes performance such as water absorption rate of intensifying regenerating aggregate before and after the processing, the results are shown in Table 8：
Intensifying regenerating aggregate performance comparison before and after the processing in the embodiment of the present invention 5 of table 8
As can be seen from Table 8, water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 7.1%, the water suction of before processing
Rate is 8.6%, and compared with before microbiological treatment, water absorption rate reduces 17.4%.After microbial augmentation, crushing refers to regeneration aggregate
Mark reduces 18.59%.
As seen from the above embodiment, using the present invention using false bacillus firmus DSM8715 intensifying regenerating aggregates
Method, the water absorption rate of obtained regeneration aggregate significantly reduce, and crush index value reduces, after preparing regeneration mortar by regeneration aggregate,
The compression strength of regeneration mortar dramatically increases.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method using false bacillus firmus DSM8715 intensifying regenerating aggregates, comprise the following steps：
1) false bacillus firmus DSM8715 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid
Preparation raw material includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3~15g/L sodium lactate and 3~10g/L hydroxide
Calcium；
2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.
2. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 1, its feature exists
In the pH value of the Mineralized Culture liquid is 10.1~10.9.
3. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 1, its feature exists
In in the mixed-culture medium that the step 1) obtains, false bacillus firmus DSM8715 bacterium number is 1 × 107~109cfu/L。
4. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 1, its feature exists
In step 1) the false bacillus firmus DSM8715 is obtained through microbiological culture media culture；
The microbiological culture media includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions；The beef extract training
Base is supported using water as solvent, including 2.5~4.5g/L beef extract and 8~15g/L peptone；3- Cyclohexylaminos-the 1- third
Sulfonate buffer includes 120~170g/L 3- Cyclohexylamino -1- propane sulfonic acid using water as solvent；3- Cyclohexylaminos-the 1- third
The pH value of sulfonate buffer is 9~10.5；The volume ratio of the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions
For (70~95):(10~20).
5. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 4, its feature exists
In the culture is carried out under oscillating condition, and the frequency of the vibration is 120~180rpm；The temperature of the culture be 25~
35 DEG C, the time of the culture is 8~48h.
6. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 4, its feature exists
In for the false bacillus firmus DSM8715 after microbiological culture media culture, centrifugation obtains bacterial sediment；With the mineralising
The nutrient solution washing bacterial sediment 2~3 times；The rotating speed of the centrifugation is 5000~7000rpm, and the time of the centrifugation is 8
~12 minutes.
7. the side of false bacillus firmus DSM8715 intensifying regenerating aggregates is utilized according to claim 1~6 any one
Method, it is characterised in that the mode of the step 2) spray is intermittent shower, sprays 0.5~1.5h every 10~12h, per 1kg again
The amount of raw aggregate spray mixed-culture medium is 1~3mL/min.
8. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 7, its feature exists
In the total time of the spray is 15~30d.
9. the side of false bacillus firmus DSM8715 intensifying regenerating aggregates is utilized according to claim 1~6 any one
Method, it is characterised in that the step 2) is replaced with into step 2 ')：The mixed culture that regeneration aggregate is obtained in the step 1)
Soaked in liquid, then spray the regeneration aggregate after the immersion with Mineralized Culture liquid step 1) described, strengthened regeneration aggregate.
10. utilizing the method for false bacillus firmus DSM8715 intensifying regenerating aggregates according to claim 9, its feature exists
In step 2 ') time of the immersion is 1~5h；Step 2 ') total time of the spray is 15~30d.
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| CN105837075A (en) * | 2015-07-07 | 2016-08-10 | 东南大学 | Method of reinforcing regenerated concrete fine aggregate with microorganism depositing calcium carbonate |
| CN107226630A (en) * | 2017-06-15 | 2017-10-03 | 太原理工大学 | The method for modifying regenerated aggregate of bacterium mineralising deposition is mixed based on aerobic basophilic |
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|---|---|---|---|---|
| CN105837075A (en) * | 2015-07-07 | 2016-08-10 | 东南大学 | Method of reinforcing regenerated concrete fine aggregate with microorganism depositing calcium carbonate |
| CN107226630A (en) * | 2017-06-15 | 2017-10-03 | 太原理工大学 | The method for modifying regenerated aggregate of bacterium mineralising deposition is mixed based on aerobic basophilic |
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