CN107601941A - A kind of microbial augmentation regeneration aggregate and preparation method thereof - Google Patents
A kind of microbial augmentation regeneration aggregate and preparation method thereof Download PDFInfo
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- CN107601941A CN107601941A CN201711057541.8A CN201711057541A CN107601941A CN 107601941 A CN107601941 A CN 107601941A CN 201711057541 A CN201711057541 A CN 201711057541A CN 107601941 A CN107601941 A CN 107601941A
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Abstract
The invention provides a kind of preparation method of microbial augmentation regeneration aggregate, comprise the following steps：1) false bacillus firmus is inoculated in microbiological culture media and cultivated, obtain microbial culture medium；2) microbial culture medium that the step 1) obtains is mixed with Mineralized Culture liquid, obtains mixed-culture medium；3) regeneration aggregate is soaked in the mixed-culture medium that the step 2) obtains, strengthened regeneration aggregate.The intensifying regenerating aggregate being prepared using the preparation method of the microbial augmentation regeneration aggregate of the present invention, water absorption rate is 4.7~6.1%, compared with before microbiological treatment, water absorption rate reduces 10.29~21.67%, crush index value reduces, after preparing regeneration mortar by intensifying regenerating aggregate, the compression strength increase by 5.39~21.84% of mortar is regenerated.
Description
Technical field
The invention belongs to technical field of concrete, more particularly to a kind of microbial augmentation regeneration aggregate and its preparation side
Method.
Background technology
With urbanization progress faster in world wide, construction industry enters the high speed development stage.A large amount of old buildings are split
Remove, generate substantial amounts of building waste, wherein discarded concrete portion is maximum.Data shows, the whole world from 1991~
Between 10 years in 2000, discarded concrete (including from the underproof product of armored concrete factory) total amount is more than 1,000,000,000 tons.I
40,000,000 tons of calculating of building waste are removed by annual by state, wherein 34% is concrete block, then resulting discarded concrete is just
There are 13,600,000 tons, and with the quickening of economic construction paces, increased trend is presented.The discarded concrete of such flood tide is except processing
Expense is surprising outer, it is also necessary to takes substantial amounts of vacant lot storage, pollutes environment, waste arable land, turn into a big public hazards in city.
Discarded concrete is not only high-quality concrete aggregate, makees to gather materials with many advantages with waste concrete block, such as
After building disintegrates, concrete block and flour sand after high-quality crushing and screening can be as the regeneration of concrete, therefore utilize discarded
Building rubbish produces regeneration aggregate and prepares the important development direction that regeneration concrete is construction industry from now on.Discarded concrete
The processing method of most worthy is exactly that it is re-used production regeneration aggregate as renewable resource, is turned waste into wealth.
Regeneration aggregate is applied with before good economic benefit, social benefit, environmental benefit and the application of good market
Scape.In the prior art, or ball mill activating and regenerating aggregate is used so that the quality of regeneration aggregate greatly improves, available for producing
Reinforced concrete member；Or using the glacial acetic acid of 5% concentration and the hydrochloric acid solution of 3% concentration to regeneration aggregate processing；Or use
Cement and Separate Fine-grained Minerals slurry liquid (such as flyash, silica flour, siliceous waterproofing agent or calcium sulphoaluminate class swelling agent) are to regeneration aggregate
The processing such as immersion, dry.But because regeneration aggregate produces hole, crack etc. during broken or processing, regeneration aggregate is caused to inhale
Water rate is larger, causes regeneration concrete hydraulic performance decline, constrains the development in regeneration aggregate production regeneration concrete field.
The content of the invention
In view of this, in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to and provide a kind of microbial augmentation regeneration
Aggregate and preparation method thereof, the water absorption rate of regeneration aggregate can be reduced.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
A kind of preparation method of microbial augmentation regeneration aggregate, comprises the following steps：
1) false bacillus firmus is inoculated in microbiological culture media and cultivated, obtain microbial culture medium；
2) microbial culture medium that the step 1) obtains is mixed with Mineralized Culture liquid, obtains mixed-culture medium；
3) regeneration aggregate is soaked in the mixed-culture medium that the step 2) obtains, strengthened regeneration aggregate.
Preferably, the microbiological culture media in the step 1) includes beef extract culture medium and 3- Cyclohexylaminos the third sulphurs of -1-
Sour culture medium, the volume ratio of the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid culture mediums is (70~95):(10~
20)。
Preferably, using water as solvent, beef extract and 8~15g/L of the beef extract culture medium including 2.5~4.5g/L
Peptone.
Preferably, using water as solvent, the 3- Cyclohexylaminos -1- propane sulfonic acid culture medium includes 10~45g/L 3- hexamethylenes
Amino -1- propane sulfonic acid；
The pH value of the 3- Cyclohexylaminos -1- propane sulfonic acid culture mediums is 10~10.5.
Preferably, the culture in the step 1) is carried out under oscillating condition, the frequency of the vibration for 120~
180rpm；
The temperature of the culture is 25~35 DEG C, and the time of the culture is 10~48h.
Preferably, using water as solvent, the Mineralized Culture liquid includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3
~15ml/L sodium lactate and 3~10g/L calcium hydroxide；
The pH value of the Mineralized Culture liquid is 10.1~10.9.
Preferably, the bacterium number of microorganism is 1 × 10 in the mixed-culture medium that the step 2) obtains7~109cfu/L。
Preferably, it is characterised in that the quality of the regeneration aggregate in the step 3) and the volume ratio of mixed-culture medium are
(0.5~1.5) g:(15~25) ml.
Preferably, the time of the step 3) immersion is 15~30 days.
The invention provides a kind of preparation method of microbial augmentation regeneration aggregate, comprise the following steps：1) will be false strong
Bacillus is inoculated in microbiological culture media and cultivated, and obtains microbial culture medium；2) microorganism for obtaining the step 1)
Nutrient solution mixes with Mineralized Culture liquid, obtains mixed-culture medium；3) mixed culture for obtaining regeneration aggregate in the step 2)
Soaked in liquid, strengthened regeneration aggregate.When regeneration aggregate soaks in mixed-culture medium, microbes will culture by metabolism
Calcium ion Ca in liquid2+It is converted into calcium carbonate CaCO3It is deposited in regeneration aggregate surface pore, so as to block regeneration aggregate surface
Hole, reduce the water absorption rate of regeneration aggregate.It is prepared using the preparation method of the microbial augmentation regeneration aggregate of the present invention
Intensifying regenerating aggregate, water absorption rate is 4.7~6.1%, compared with before microbiological treatment, water absorption rate reduces 10.29~
21.67%, crush index value reduces, and after preparing regeneration mortar by intensifying regenerating aggregate, regenerates the compression strength increase of mortar
5.39~21.84%.
Embodiment
The invention provides a kind of preparation method of microbial augmentation regeneration aggregate, comprise the following steps：
1) false bacillus firmus is inoculated in microbiological culture media and cultivated, obtain microbial culture medium；
2) microbial culture medium that the step 1) obtains is mixed with Mineralized Culture liquid, obtains mixed-culture medium；
3) regeneration aggregate is soaked in the mixed-culture medium that the step 2) obtains, strengthened regeneration aggregate.
In the present invention, the numbering of the false bacillus firmus strain is DSM8715, from Chinese General Microbiological Culture
Preservation administrative center obtains.
In the present invention, the microbiological culture media preferably includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid
The volume ratio of culture medium, the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid culture mediums is preferably (70~95):(10
~20), more preferably (80~90):(12~18), most preferably 85:15.
In the present invention, using water as solvent, the beef extract culture medium preferably includes 2.5~4.5g/L beef extract and 8
The beef extract of~15g/L peptone, more preferably 3.0~4.0g/L and 10~13g/L peptone, most preferably 3.53g/
L beef extract and 11.76g/L peptone.
In the present invention, used after the preferred sterilizing of the beef extract culture medium；The sterilizing is preferably high-temperature sterilization, described
The temperature of sterilizing is preferably 121 DEG C, and the time of the sterilizing is preferably 15min.The present invention is to the equipment of the sterilizing without spy
It is different to require, using those skilled in the art's conventional sterilant equipment, such as high-pressure steam sterilizing pan.
In the present invention, using water as solvent, the 3- Cyclohexylaminos -1- propane sulfonic acid culture mediums preferably include 10~45g/L
3- Cyclohexylamino -1- propane sulfonic acid, more preferably 15~30g/L, most preferably 22.13g/L.
In the present invention, the pH value of the 3- Cyclohexylaminos -1- propane sulfonic acid culture mediums is preferably 10~10.5.
In the present invention, used after the preferred sterilizing of the 3- Cyclohexylaminos -1- propane sulfonic acid culture medium；The sterilizing is preferably
High-temperature sterilization, the temperature of the sterilizing is preferably 121 DEG C, and the time of the sterilizing is preferably 15min.The present invention is to the sterilizing
Equipment there is no particular/special requirement, using those skilled in the art's conventional sterilant equipment, such as high-pressure steam sterilizing pan.
The present invention is by beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid culture mediums aseptically according to above-mentioned body
Product obtains microbiological culture media than mixing.
False bacillus firmus is inoculated in microbiological culture media by the present invention to be cultivated, and obtains microbial culture medium.
The present invention does not have particular/special requirement to the inoculum concentration of the false bacillus firmus, specially normal according to the art with oese
The a little colony inoculation of operation picking of rule is in microbiological culture media.
In the present invention, the culture is preferably carried out under oscillating condition, and the frequency of the vibration is preferably 120~
180rpm, more preferably 130~160rpm, most preferably 150rpm.In the present invention, the temperature of the culture be preferably 25~
35 DEG C, more preferably 28~32 DEG C, most preferably 29~31 DEG C.In the present invention, the time of the culture is preferably 8~48h,
More preferably 10~24h, most elect 12~20h as.The present invention is not particularly limited to the instrument of the shaken cultivation, using this
The equipment that art personnel routinely select, such as constant-temperature table.
After obtaining microbial culture medium, the present invention mixes the microbial culture medium with Mineralized Culture liquid, is mixed
Nutrient solution.In the present invention, using water as solvent, the Mineralized Culture liquid preferably includes 10~45g/L 3- Cyclohexylaminos -1- third
3- Cyclohexylaminos-the 1- third of the calcium hydroxide of sulfonic acid, 3~15ml/L sodium lactate and 3~10g/L, more preferably 15~35g/L
3- Cyclohexylaminos the third sulphurs of -1- of the calcium hydroxide of sulfonic acid, 5~10ml/L sodium lactate and 4~8g/L, most preferably 22.13g/L
The calcium hydroxide of acid, 7.5ml/L sodium lactate and 6.67g/L.
In the present invention, the pH value of the Mineralized Culture liquid is preferably 10.1~10.9, and more preferably 10.4~10.6.This
Regulation of the invention to the pH value is preferably adjusted using acetum, and the mass concentration of acetic acid is preferably 10 in the acetum
~100%, more preferably 50~99%, most preferably 80~95%.
In the present invention, the microbial culture medium is mixed with Mineralized Culture liquid, obtains mixed-culture medium.The mixing
In nutrient solution, the bacterium number of false bacillus firmus is preferably 1 × 107~109Cfu/L, more preferably 1 × 108cfu/L。
After obtaining mixed-culture medium, regeneration aggregate is placed in the mixed-culture medium by the present invention to be soaked, and is strengthened again
Raw aggregate.In the present invention, the granularity of the regeneration aggregate is preferably 0.10~25mm.In embodiments of the present invention, Regenerated Bone
The granularity of material is 0.15~4.75mm or 10~20mm.
The present invention does not have special limitation to the source of the regeneration aggregate, using technology well known to those skilled in the art
Scheme is prepared by discarded concrete.Specifically, in an embodiment of the present invention, the preparation method of the regeneration aggregate is preferred
Comprise the following steps：
1) discarded concrete is crushed；
2) reinforcing bar in concrete after the step 1) is crushed takes out, and obtains mass concrete；
3) mass concrete for obtaining the step 2) crushes again, obtains 0.10~25mm regeneration aggregate.
In the present invention, the broken degree of the mass concrete is for 10~20mm or less than 4.75mm；The step
1) mode crushed in is to be broken off reinforcing bar or to take out reinforcing bar after hand breaking, obtain no-reinforcing-bar coagulation using quartering hammer
Soil.
In the present invention, the quality of the regeneration aggregate and the volume ratio of mixed-culture medium are preferably (0.5~1.5) g:
(15~25) ml, more preferably (0.8~1.2) g:(18~22) ml, most preferably 1g:20ml.
In the present invention, the time of the immersion is preferably 15~30 days, more preferably 18~25 days, most preferably 20
My god.In the present invention, when regeneration aggregate soaks in mixed-culture medium, microbes are by being metabolized the calcium ion in nutrient solution
Ca2+It is converted into calcium carbonate CaCO3It is deposited in regeneration aggregate surface pore, so as to block regeneration aggregate surface pore, reduces again
The water absorption rate of raw aggregate.In the present invention, the Mineralized Culture liquid act as offer calcium source (calcium ion) in immersion process
Suitable living environment and higher mineralization activity with microorganism.
After the immersion, the present invention preferably dries the regeneration aggregate after the immersion, and strengthened regeneration aggregate.This hair
The bright method to the drying is not particularly limited, and the technical scheme dried using aggregate well known to those skilled in the art is
Can；In an embodiment of the present invention, can be specifically dried using air dry oven or naturally dry；When the drying is preferred
For forced air drying when, the drying is preferably specially：Dried under the conditions of 25~35 DEG C to constant weight.
In the present invention, according to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》At test microbes
The water absorption rate change of intensifying regenerating aggregate before and after reason.
A kind of microbial augmentation regeneration aggregate provided by the invention and preparation method thereof is carried out with reference to embodiment detailed
Thin explanation, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, crushed reinforcing bar using quartering hammer
Out, the difficult reinforcing bar taken out after hand breaking by taking out, the discarded concrete tentatively crushed, then is carried out with jaw crusher
It is broken, screen out 10~20mm aggregate.
The preparation of Mineralized Culture liquid：Calcium hydroxide 6.67g is weighed, 3- Cyclohexylamino -1- propane sulfonic acid 22.13g is weighed, measures
Pfansteihl sodium 7.5mL, deionized water 830mL, 850mL is settled to 10.5 using vinegar acid for adjusting pH value after fully mixing.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；Weigh 3- Cyclohexylamino -1- propane sulfonic acid 22.3g, measure from
Sub- water 130mL, pH value is adjusted to 10.5 with 6mol/L sodium hydroxides after fully mixing, 150mL is settled to, prepares 3- hexamethylene ammonia
Base -1- propane sulfonic acid culture mediums, 121 DEG C of sterilizing 15min, are cooled to room temperature；It will be cooled to the beef extract culture medium and 3- rings of room temperature
Own amino -1- propane sulfonic acid culture medium mixing, obtains microbiological culture media.
False bacillus firmus is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C, the frequency of vibration
Rate is 150rpm, and culture obtains microbial culture medium after terminating, and then adds in Mineralized Culture liquid microbial culture medium, obtains
Mixed-culture medium, it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；Regeneration aggregate is weighed to be added in mixed-culture medium,
The quality of regeneration aggregate and the volume ratio of mixed-culture medium are 1g:20ml, soaked 20 days under normal temperature condition, strengthened regeneration
Aggregate.According to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》Test microbes intensifying regenerating before and after the processing
The performances such as the water absorption rate of aggregate, the results are shown in Table 1：
Intensifying regenerating aggregate performance comparison before and after the processing in the embodiment of the present invention of table 1
It can be drawn by table 1, water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 6.1%, the water suction of before processing
Rate is 6.8%, and compared with before microbiological treatment, water absorption rate reduces 10.29%.
Embodiment 2
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, crushed reinforcing bar using quartering hammer
Out, the difficult reinforcing bar taken out after hand breaking by taking out, the discarded concrete tentatively crushed, then is carried out with jaw crusher
It is broken, the aggregate less than 10mm is screened out, recycles composite crusher to carry out two-stage crushing, screens out the bone less than 4.75mm
Material, finally prepare 015~4.75mm aggregate.
The preparation of Mineralized Culture liquid：Calcium hydroxide 6.67g is weighed, 3- Cyclohexylamino -1- propane sulfonic acid 22.13g is weighed, measures
Pfansteihl sodium 7.5mL, deionized water 830mL, 850mL is settled to 10.5 using vinegar acid for adjusting pH value after fully mixing.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；Weigh 3- Cyclohexylamino -1- propane sulfonic acid 22.3g, measure from
Sub- water 130mL, pH value is adjusted to 10.5 with 6mol/L sodium hydroxides after fully mixing, 150mL is settled to, prepares 3- hexamethylene ammonia
Base -1- propane sulfonic acid culture mediums, 121 DEG C of sterilizing 15min, are cooled to room temperature；It will be cooled to the beef extract culture medium and 3- rings of room temperature
Own amino -1- propane sulfonic acid culture medium mixing, obtains microbiological culture media.
False bacillus firmus is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C, the frequency of vibration
Rate is 150rpm, and culture obtains microbial culture medium after terminating, and then adds in Mineralized Culture liquid microbial culture medium, obtains
Mixed-culture medium, it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；Regeneration aggregate is weighed to be added in mixed-culture medium,
The quality of regeneration aggregate and the volume ratio of mixed-culture medium are 1g:20ml, soaked 20 days under normal temperature condition, strengthened regeneration
Aggregate.According to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》Test microbes intensifying regenerating before and after the processing
The performances such as the water absorption rate of aggregate, as a result see the table below：
The granularity of intensifying regenerating aggregate in the embodiment of the present invention 2 of table 2
Intensifying regenerating aggregate is through the performance before and after microbiological treatment in the embodiment of the present invention 2 of table 3
It can be drawn by table 3, the water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 4.7%, the suction of before processing
Water rate is 6.0%, and compared with before microbiological treatment, water absorption rate reduces 21.67%.
Intensifying regenerating aggregate crush index in the embodiment of the present invention 2 of table 4
It can be drawn by table 4, after microbiological treatment, crush index value reduces.
Embodiment 3
Regeneration mortar is prepared by the use of the intensifying regenerating aggregate that embodiment 2 obtains as reclaimed sand, proportioning is shown in Table 5, investigates micro- life
The regeneration mortar compression strength of thing before and after the processing, the results are shown in Table 6.
The mortar mix ratio of table 5
Table 6 regenerates mortar compression strength/MPa
It can be drawn by table 6, after microbiological treatment, the compression strength for regenerating mortar adds 5.39~21.84%.
As seen from the above embodiment, using the preparation method of microbial augmentation regeneration aggregate of the invention, obtained regeneration
The water absorption rate of aggregate reduces 10.29~21.67%, and crush index value reduces, after preparing regeneration mortar by regeneration aggregate, regeneration
The compression strength increase by 5.39~21.84% of mortar.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of preparation method of microbial augmentation regeneration aggregate, comprises the following steps：
1) false bacillus firmus is inoculated in microbiological culture media and cultivated, obtain microbial culture medium；
2) microbial culture medium that the step 1) obtains is mixed with Mineralized Culture liquid, obtains mixed-culture medium；
3) regeneration aggregate is soaked in the mixed-culture medium that the step 2) obtains, strengthened regeneration aggregate.
2. microbial augmentation regeneration aggregate method according to claim 1, it is characterised in that micro- life in the step 1)
Thing culture medium includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid culture mediums, the beef extract culture medium and 3- hexamethylenes
The volume ratio of amino -1- propane sulfonic acid culture mediums is (70~95):(10~20).
3. microbial augmentation regeneration aggregate method according to claim 2, it is characterised in that using water as solvent, the ox
Meat extract culture medium includes 2.5~4.5g/L beef extract and 8~15g/L peptone.
4. microbial augmentation regeneration aggregate method according to claim 2, it is characterised in that using water as solvent, the 3-
Cyclohexylamino -1- propane sulfonic acid culture medium includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid；
The pH value of the 3- Cyclohexylaminos -1- propane sulfonic acid culture mediums is 10~10.5.
5. microbial augmentation regeneration aggregate method according to claim 1, it is characterised in that the culture in the step 1)
Carried out under oscillating condition, the frequency of the vibration is 120~180rpm；
The temperature of the culture is 25~35 DEG C, and the time of the culture is 10~48h.
6. microbial augmentation regeneration aggregate method according to claim 1, it is characterised in that using water as solvent, the ore deposit
Changing nutrient solution includes 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 3~15ml/L sodium lactate and 3~10g/L hydrogen-oxygen
Change calcium；
The pH value of the Mineralized Culture liquid is 10.1~10.9.
7. microbial augmentation regeneration aggregate method according to claim 1, it is characterised in that the step 2) obtains mixed
The bacterium number for closing microorganism in nutrient solution is 1 × 107~109cfu/L。
8. the microbial augmentation regeneration aggregate method according to claim 1,6 or 7, it is characterised in that in the step 3)
The quality of regeneration aggregate and the volume ratio of mixed-culture medium be (0.5~1.5) g:(15~25) ml.
9. microbial augmentation regeneration aggregate method according to claim 1, it is characterised in that the step 3) immersion when
Between be 15~30 days.
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| CN111689738A (en) * | 2020-07-01 | 2020-09-22 | 台州四强新型建材有限公司 | Environment-friendly recycled concrete and preparation process thereof |
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| CN103509784A (en) * | 2013-09-18 | 2014-01-15 | 深圳大学 | Screening method of mineralized microorganisms for self-repairing of concrete cracks |
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Application publication date: 20180119 |
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| RJ01 | Rejection of invention patent application after publication |