CN107804987A - A kind of method that high-performance regeneration aggregate is prepared using bacillus B6 - Google Patents
A kind of method that high-performance regeneration aggregate is prepared using bacillus B6 Download PDFInfo
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- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
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- C04B20/00—Use of materials as fillers for mortars, concrete or artificial stone according to more than one of groups C04B14/00 - C04B18/00 and characterised by shape or grain distribution; Treatment of materials according to more than one of the groups C04B14/00 - C04B18/00 specially adapted to enhance their filling properties in mortars, concrete or artificial stone; Expanding or defibrillating materials
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Abstract
The invention provides a kind of method that high-performance regeneration aggregate is prepared using bacillus B6：1) bacillus B6 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid includes the 10~45g/L propane sulfonic acid of 3 Cyclohexylamino 1,4~12g/L sodium lactate, 1~3g/L sodium nitrate, 20~40mmol/L calcium chloride, 0.5~1.5mmol/L magnesium sulfate, 0.08~0.18mmol/L potassium dihydrogen phosphate；2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.The regeneration aggregate being prepared using the method for the invention, water absorption rate and crush index value are significantly reduced；The regeneration mortar compression strength prepared by the intensifying regenerating aggregate dramatically increases.
Description
Technical field
The invention belongs to technical field of concrete, more particularly to one kind to prepare high-performance Regenerated Bone using bacillus B6
The method of material.
Background technology
With urbanization progress faster in world wide, construction industry enters the high speed development stage.A large amount of old buildings are split
Remove, generate substantial amounts of building waste, wherein discarded concrete portion is maximum.Data shows, the whole world from 1991~
Between 10 years in 2000, discarded concrete (including from the underproof product of armored concrete factory) total amount is more than 1,000,000,000 tons.I
40,000,000 tons of calculating of building waste are removed by annual by state, wherein 34% is concrete block, then resulting discarded concrete is just
There are 13,600,000 tons, and with the quickening of economic construction paces, increased trend is presented.The discarded concrete of such flood tide is except processing
Expense is surprising outer, it is also necessary to takes substantial amounts of vacant lot storage, pollutes environment, waste arable land, turn into a big public hazards in city.
Discarded concrete is not only high-quality concrete aggregate, makees to gather materials with many advantages with waste concrete block, such as
After building disintegrates, concrete block and flour sand after high-quality crushing and screening can be as the regeneration of concrete, therefore utilize discarded
Building rubbish produces regeneration aggregate and prepares the important development direction that regeneration concrete is construction industry from now on.Discarded concrete
The processing method of most worthy is exactly that it is re-used production regeneration aggregate as renewable resource, is turned waste into wealth.
Regeneration aggregate is applied with before good economic benefit, social benefit, environmental benefit and the application of good market
Scape.In the prior art, or ball mill activating and regenerating aggregate is used so that the quality of regeneration aggregate greatly improves, available for producing
Reinforced concrete member；Or using the glacial acetic acid of 5% concentration and the hydrochloric acid solution of 3% concentration to regeneration aggregate processing；Or use
Cement and Separate Fine-grained Minerals slurry liquid (such as flyash, silica flour, siliceous waterproofing agent or calcium sulphoaluminate class swelling agent) are to regeneration aggregate
The processing such as immersion, dry.But because regeneration aggregate produces hole, crack etc. during broken or processing, regeneration aggregate is caused to inhale
Water rate is larger, causes regeneration concrete hydraulic performance decline, constrains the development in regeneration aggregate production regeneration concrete field.
The content of the invention
In view of this, in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to which water absorption rate and pressure can be reduced by providing one kind
Broken index, improve the method that high-performance regeneration aggregate is prepared using bacillus B6 of mixed mortar compression strength.
The invention provides a kind of method that high-performance regeneration aggregate is prepared using bacillus B6, comprise the following steps：
1) bacillus B6 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid include 10~
The chlorination of 45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 4~12g/L sodium lactate, 1~3g/L sodium nitrate, 20~40mmol/L
Calcium, 0.5~1.5mmol/L magnesium sulfate, 0.08~0.18mmol/L potassium dihydrogen phosphate；The biology guarantor of the bacillus B6
It is CGMCC No.13360 to hide numbering；
2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.
Preferably, the pH value of the Mineralized Culture liquid is 10.1~10.9.
Preferably, in the mixed-culture medium that the step 1) obtains, bacillus B6 bacterium number is 1 × 107~109cfu/
L。
Preferably, step 1) the bacillus B6 is obtained through microbiological culture media culture；
The microbiological culture media includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions；The beef
Cream culture medium is using water as solvent, including 2.5~4.5g/L beef extract and 8~15g/L peptone；The 3- Cyclohexylaminos-
1- propane sulfonic acid buffer solution includes 120~170g/L 3- Cyclohexylamino -1- propane sulfonic acid using water as solvent.
Preferably, the pH value of the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solutions is 9~10.5.
Preferably, the volume ratio of the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions is (70~95):
(10~20).
Preferably, the temperature of the culture is 25~35 DEG C, and the time of the culture is 8~48h.
Preferably, the culture is carried out under oscillating condition, and the frequency of the vibration is 120~180rpm.
Preferably, the mode of the step 2) spray is intermittent shower, 0.5~1.5h is sprayed every 10~12h, per 1kg
The amount of regeneration aggregate spray mixed-culture medium is 1~3mL/min.
Preferably, the total time of the spray is 15~30d.
Beneficial effect：
The invention provides a kind of method that high-performance regeneration aggregate is prepared using bacillus B6, comprise the following steps：
1) bacillus B6 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid includes 10~45g/L 3-
Cyclohexylamino -1- propane sulfonic acid, 4~12g/L sodium lactate, 1~3g/L sodium nitrate, 20~40mmol/L calcium chloride, 0.5~
1.5mmol/L magnesium sulfate, 0.08~0.18mmol/L potassium dihydrogen phosphate；2) mixed-culture medium obtained using step 1) is sprayed
Regeneration aggregate is drenched, strengthened regeneration aggregate.After the spray of the blended nutrient solution of regeneration aggregate, microbes are attached to Regenerated Bone
Expect in surface pore, using the composition in Mineralized Culture liquid, by being metabolized calcium ion Ca therein2+It is converted into calcium carbonate
CaCO3It is deposited in regeneration aggregate surface pore, so as to block regeneration aggregate surface pore, reduces the water absorption rate of regeneration aggregate.
For the regeneration aggregate being prepared using the method for the invention compared with before microbiological treatment, water absorption rate reduces 33.3%；Pressure
Broken index is significantly reduced.The regeneration mortar compression strength prepared by the intensifying regenerating aggregate significantly improves.
Biological deposits explanation：
Bacillus B6 (Bacillus sp.B6), Chinese microorganism strain preservation pipe is deposited on November 30th, 2016
Reason committee common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and Chinese Academy of Sciences microorganism is ground
Study carefully institute, biological deposits numbering is CGMCCNo.13360.
Embodiment
The invention provides a kind of method of microbial augmentation regeneration aggregate, comprise the following steps：
1) bacillus B6 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid include 10~
The chlorination of 45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 4~12g/L sodium lactate, 1~3g/L sodium nitrate, 20~40mmol/L
Calcium, 0.5~1.5mmol/L magnesium sulfate, 0.08~0.18mmol/L potassium dihydrogen phosphate；The biology guarantor of the bacillus B6
It is CGMCC No.13360 to hide numbering；
2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.
In the present invention, the Latin of the bacillus B6 is entitled：Bacillussp.B6, in China General Microbiological bacterium
The preserving number of kind of preservation administrative center (abbreviation CGMCC) is：CGMCC NO.13360.
In the present invention, the bacillus B6 preferably obtains through microbiological culture media culture；The microbiological culture media
Preferably include beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid (CAPS) buffer solution.Beef extract culture medium is the work of strain
Change and nutritional ingredient is provided, CAPS buffer solutions are maintaining the pH of microbiological culture media stable.
In the present invention, using water as solvent, the beef extract culture medium preferably includes 2.5~4.5g/L beef extract and 8
The beef extract of~15g/L peptone, more preferably 3.0~4.0g/L and 10~13g/L peptone, most preferably 3.53g/
L beef extract and 11.76g/L peptone.
In the present invention, used after the preferred sterilizing of the beef extract culture medium；The sterilizing is preferably high-temperature sterilization, described
The temperature of sterilizing is preferably 121 DEG C, and the time of the sterilizing is preferably 15min.The present invention is to the equipment of the sterilizing without spy
It is different to require, using those skilled in the art's conventional sterilant equipment, such as high-pressure steam sterilizing pan.
In the present invention, using water as solvent, the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solutions preferably include 120~170g/
L 3- Cyclohexylamino -1- propane sulfonic acid, most preferably more preferably 140~160g/L, 147.5g/L.
In the present invention, the pH value of the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solutions is preferably 9~10.5, more preferably
9.5~10.The method that the present invention adjusts pH value to 3- Cyclohexylamino -1- propane sulfonic acid buffer solution is not particularly limited, using ability
The Normal practice of field technique personnel.In the specific embodiment of the invention, preferably 3- Cyclohexylaminos -1- third is adjusted with NaOH
The pH value of sulfonate buffer.The NaOH is preferably the NaOH aqueous solution, and the concentration of the NaOH aqueous solution is preferably 1~10mol/
L, more preferably 5~7mol/l.
In the present invention, used after the preferred sterilizing of the 3- Cyclohexylaminos -1- propane sulfonic acid buffer solution；The sterilizing is preferably
High-temperature sterilization, the temperature of the sterilizing is preferably 121 DEG C, and the time of the sterilizing is preferably 15min.The present invention is to the sterilizing
Equipment there is no particular/special requirement, using those skilled in the art's conventional sterilant equipment, such as high-pressure steam sterilizing pan.
In the present invention, the volume ratio of the beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions is preferably
(70~95):(10~20), more preferably (80~90):(12~18), most preferably 85:15.The present invention is by beef extract culture
Base and 3- Cyclohexylamino -1- propane sulfonic acid buffer solution aseptically mix according to above-mentioned volume ratio, obtain pH value as 9.5~10
Microbiological culture media.The pH that the present invention first adjusts 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions mixes with beef extract culture medium again
Purpose be avoid sodium hydroxide solution strong basicity destroy beef extract culture medium in nutritional ingredient.
Bacillus B6 is inoculated in microbiological culture media by the present invention to be cultivated, and obtains microbial culture medium.The present invention is right
The inoculum concentration of the bacillus B6 does not have particular/special requirement, specially with oese according to the conventional operation picking of the art
A little colony inoculation is in microbiological culture media.
In the present invention, the culture is preferably carried out under oscillating condition, and the frequency of the vibration is preferably 120~
180rpm, more preferably 130~160rpm, most preferably 150rpm.In the present invention, the temperature of the culture be preferably 25~
35 DEG C, more preferably 28~32 DEG C, most preferably 29~31 DEG C.In the present invention, the time of the culture is preferably 8~48h,
More preferably 10~24h, most preferably 12~20h.The present invention is not particularly limited to the instrument of the shaken cultivation, using this
The equipment that art personnel routinely select, such as constant-temperature table.
In the present invention, for the bacillus B6 after microbiological culture media culture, centrifugation obtains bacterial sediment；In this hair
In bright, the rotating speed of the centrifugation is preferably 5000~7000rpm, more preferably 6000rpm；The time of the centrifugation be 8~
12min, more preferably 10min.After centrifugation, the bacterial sediment is washed 2~3 times with the Mineralized Culture liquid.The present invention uses
The purpose of Mineralized Culture liquid washing thalline precipitation is the further beef extract and peptone component removed in microbiological culture media, from
And during avoiding follow-up Mineralized Culture, the composition such as beef extract, peptone is attached to regeneration aggregate surface, influences microorganism to again
The reinforcing of raw aggregate.
The present invention mixes bacillus B6 with Mineralized Culture liquid, obtains mixed-culture medium.In the present invention, the mineralising
Nutrient solution using water as solvent, preferably include 10~45g/L 3- Cyclohexylamino -1- propane sulfonic acid, 4~12g/L sodium lactate, 1~
3g/L sodium nitrate, 20~40mmol/L calcium chloride, 0.5~1.5mmol/L magnesium sulfate, 0.08~0.18mmol/L phosphoric acid
3- Cyclohexylamino -1- propane sulfonic acid, 6~10g/L sodium lactate, the 1.5~2g/L nitric acid of potassium dihydrogen, more preferably 15~35g/L
Sodium, 25~35mmol/L calcium chloride, 0.8~1.2mmol/L magnesium sulfate, 0.10~0.15mmol/L potassium dihydrogen phosphate,
Most preferably 22.13g/L 3- Cyclohexylamino -1- propane sulfonic acid, 8.5g/L sodium lactate, 1.7g/L sodium nitrate, 25.5mmol/L
Calcium chloride, 0.85mmol/L magnesium sulfate, 0.11mmol/L potassium dihydrogen phosphate.In the present invention, the 3- Cyclohexylaminos-
1- propane sulfonic acid maintains the pH stable of the Mineralized Culture liquid as composition is buffered；The sodium lactate provides carbon source for microorganism,
Metabolism produces carbonate or carbon dioxide after microorganism absorbs, and reacts generation calcium carbonate with calcium ion；The sodium nitrate is micro-
Biology provides nitrogen source, for bacterium synthetic protein, nucleic acid and other nitrogen compounds；The calcium chloride provides for mineralising deposition
Calcium ion, carbonate or carbon dioxide reaction generation calcium carbonate with microbial metabolism generation；The magnesium sulfate and biphosphate
Potassium provides inorganic salts ingredients for microorganism, in the growth course of microorganism, forms the Cell Component of thalline, the composition as enzyme
Partly, the activator or inhibitor of enzyme, adjusts osmotic pressure, PH, oxidation-reduction potential of culture medium etc..
In the present invention, the Mineralized Culture liquid is preferably prepared using two-step method.First making lactic acid sodium, sodium nitrate,
The mixed solution (A liquid) of calcium chloride, magnesium sulfate and potassium dihydrogen phosphate, then the water by the A liquid and 3- Cyclohexylamino -1- propane sulfonic acid
Solution (B liquid) is mixed, and obtains Mineralized Culture liquid.The pH value of A liquid or B liquid of the present invention is preferably 10.1~10.9, more
Preferably 10.4~10.6, most preferably 10.5.The present invention individually adjusts the pH value of A liquid and B liquid in process for preparation；This hair
The bright pH value that A liquid and B liquid are preferably adjusted with NaOH.Being adjusted according to NaOH solution, the concentration of the NaOH solution is preferably 1~
10mol/l, more preferably 5~7mol/l.After A liquid and B liquid are mixed, Mineralized Culture liquid is obtained；The pH of the Mineralized Culture liquid
Value is preferably 10.1~10.9, more preferably 10.4~10.6, most preferably 10.5.The present invention prepares mineralising training using two-step method
Nutrient solution, the pH of A liquid and B liquid is adjusted respectively, it is easier to control ph, so as to provide suitable pH for bacterial metabolism.Ore deposit of the present invention
When the pH value for changing nutrient solution is 10.5, bacterial growth is not only improved, is advantageous to synthetic calcium carbonate again.
The present invention mixes bacillus B6 with Mineralized Culture liquid, obtains mixed-culture medium.In the mixed-culture medium,
Bacillus B6 bacterium number is preferably 1 × 107~109Cfu/L, more preferably 1 × 108cfu/L。
The present invention makes bacillus B6 be attached to the surface pore of regeneration aggregate using mixed-culture medium spray regeneration aggregate
In, while microbes are by being metabolized the calcium ion Ca in nutrient solution2+It is converted into calcium carbonate CaCO3It is deposited on regeneration aggregate table
In face gap, so as to block regeneration aggregate surface pore, the water absorption rate of regeneration aggregate is reduced.In the present invention, the Regenerated Bone
The granularity of material is preferably 0.10~25mm.In an embodiment of the present invention, the granularity of regeneration aggregate be 0.15~4.75mm or 5~
10mm。
The present invention does not have special limitation to the source of the regeneration aggregate, using technology well known to those skilled in the art
Scheme is prepared by discarded concrete.Specifically, in an embodiment of the present invention, the preparation method of the regeneration aggregate is preferred
Comprise the following steps：
Discarded concrete is crushed, the reinforcing bar in the concrete after crushing is taken out, obtains mass concrete；By the nothing
Armored concrete crushes again, obtains 0.10~25mm regeneration aggregate.
In the present invention, the broken degree of the mass concrete is for 4.75~20mm or less than 4.75mm；It is described useless
The mode for abandoning concrete disintegrating is preferably to be broken off reinforcing bar or to take out reinforcing bar after hand breaking, obtain nothing using quartering hammer
Armored concrete.The present invention is not particularly limited to the mode that mass concrete crushes again, using the routine in this area
Method.
The present invention is using on mixed-culture medium spray to regeneration aggregate, and strengthened regeneration aggregate.In the present invention, it is described
Spray is to keep the surface wettability of regeneration aggregate as standard, it is preferred to use the mode of intermittent shower, 0.5 is sprayed every 10~12h
~1.5h, the amount per 1kg regeneration aggregates spray mixed-culture medium is 1~3mL/min；It is furthermore preferred that 1h is sprayed every 11h, often
The amount of 1kg regeneration aggregates spray mixed-culture medium is 2mL/min.During spray, keep the temperature of Mineralized Culture liquid for 25~
35 DEG C, more preferably 28~32 DEG C, most preferably 29~31 DEG C.
The total time of the spray is 15~30d, more preferably 18~25d, most preferably 20d.Wherein, spray it is total when
Between include spray time and spray off time.In the present invention, the purpose for spraying mixed-culture medium is to keep regeneration aggregate
Moistening, provide moisture and nutriment for the vital movement of microorganism.Meanwhile microorganism is by being metabolized the calcium in nutrient solution
Ion Ca2+It is converted into calcium carbonate CaCO3It is deposited in regeneration aggregate surface pore, so as to block regeneration aggregate surface pore, drop
The water absorption rate of low regeneration aggregate.In the present invention, the Mineralized Culture liquid during spray act as provide calcium source (calcium from
Son) and the suitable living environment of microorganism and higher mineralization activity.
After the regeneration aggregate that strengthened, preferred pair regeneration aggregate is dried the present invention.The present invention is to the drying
Method is not particularly limited, the technical scheme dried using aggregate well known to those skilled in the art；In the reality of the present invention
Apply in example, can be specifically dried using air dry oven or naturally dry.When the drying is preferably forced air drying, institute
State to dry and be preferably specially：Dried under the conditions of 25~35 DEG C to constant weight.
In the present invention, according to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》At test microbes
The water absorption rate change of intensifying regenerating aggregate before and after reason.
A kind of microbial augmentation regeneration aggregate provided by the invention and preparation method thereof is carried out with reference to embodiment detailed
Thin explanation, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1：
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, reinforcing bar is broken off to come, hardly possible is taken out
Reinforcing bar by being taken out after hand breaking, the mass concrete tentatively crushed, then crushed with jaw crusher, sieve
Go out 4.75~20mm aggregate, continuous grading.
The preparation of Mineralized Culture liquid：By 8.5g sodium lactates, 1.7g sodium nitrate, 25.5mmol calcium chloride, 0.85mmol sulfuric acid
Magnesium and 0.11mmol potassium dihydrogen phosphates are dissolved in 800ml deionized waters, after fully mixing, are settled to 850mL, are obtained A liquid；Weigh
3- Cyclohexylamino -1- propane sulfonic acid 22.3g, measure deionized water 130mL, with 6mol/L sodium hydroxides and 90% after fully mixing
Acetum adjusts pH value to 10.5, is settled to 150mL, obtains B liquid；A liquid is well mixed with B liquid, as Mineralized Culture liquid.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionization
Water 130mL, pH value is adjusted to 10 with 6mol/L sodium hydroxides after fully mixing, 150mL is settled to, prepares 3- Cyclohexylaminos -1-
Propane sulfonic acid buffer solution, 121 DEG C of sterilizing 15min, is cooled to room temperature；It will be cooled to the beef extract culture medium and 3- hexamethylene ammonia of room temperature
Base -1- propane sulfonic acid buffer solution mixes, and obtains microbiological culture media.
Bacillus B6 is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C, the frequency of vibration is
150rpm, culture are centrifuged after terminating and washed 3 times with Mineralized Culture liquid, obtain bacterial precipitation, bacterial precipitation is added into mineralising
In nutrient solution, mixed-culture medium is obtained, it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；Will mixing under normal temperature condition
Nutrient solution is sprayed to regeneration aggregate, and 1h is sprayed every 11h, and the amount per 1kg regeneration aggregates spray mixed-culture medium is 2mL/min,
Intermittent shower 20 days, strengthened regeneration aggregate.According to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》
The test microbes performance such as water absorption rate of intensifying regenerating aggregate before and after the processing, the results are shown in Table 1：
Intensifying regenerating aggregate performance comparison before and after the processing in the embodiment of the present invention of table 1
It can be drawn by table 1, water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 3.9%, the water suction of before processing
Rate is 8.6%, and compared with before microbiological treatment, water absorption rate reduces 54.7%.
Embodiment 2：
The preparation of regeneration aggregate：Discarded concrete tentatively crush with quartering hammer, reinforcing bar is broken off to come, hardly possible is taken out
Reinforcing bar by being taken out after hand breaking, the mass concrete tentatively crushed, then crushed with jaw crusher, sieve
Go out to be less than 10mm aggregate, recycle composite crusher to carry out two-stage crushing, screen out the aggregate less than 4.75mm, finally prepare
Go out 0.15~4.75mm aggregate.
The preparation of Mineralized Culture liquid：By 8.5g sodium lactates, 1.7g sodium nitrate, 25.5mmol calcium chloride, 0.85mmol sulfuric acid
Magnesium and 0.11mmol potassium dihydrogen phosphates are dissolved in 800ml deionized waters, after fully mixing, are settled to 850mL, are obtained A liquid；Weigh
3- Cyclohexylamino -1- propane sulfonic acid 22.3g, measure deionized water 130mL, with 6mol/L sodium hydroxides and 90% after fully mixing
Acetum adjusts pH value to 10.5, is settled to 150mL, obtains B liquid；A liquid is well mixed with B liquid, as Mineralized Culture liquid.
The preparation of microbiological culture media：Beef extract 3g, peptone 10g are weighed, deionized water 850mL is measured, is configured to ox
Meat extract culture medium, 121 DEG C of sterilizing 15min, is cooled to room temperature；3- Cyclohexylamino -1- propane sulfonic acid 22.3g are weighed, measure deionization
Water 130mL, pH value is adjusted to 10 with 6mol/L sodium hydroxides after fully mixing, 150mL is settled to, prepares 3- Cyclohexylaminos -1-
Propane sulfonic acid buffer solution, 121 DEG C of sterilizing 15min, is cooled to room temperature；It will be cooled to the beef extract culture medium and 3- hexamethylene ammonia of room temperature
Base -1- propane sulfonic acid buffer solution mixes, and obtains microbiological culture media.
Bacillus B6 is inoculated in microbiological culture media, the shaken cultivation 24h under the conditions of 30 DEG C, the frequency of vibration is
150rpm, culture are centrifuged after terminating and washed 3 times with Mineralized Culture liquid, obtain bacterial precipitation, bacterial precipitation is added into mineralising
In nutrient solution, mixed-culture medium is obtained, it is 1 × 10 to make the bacterium number in mixed-culture medium8cfu/L；Will mixing under normal temperature condition
Nutrient solution is sprayed to regeneration aggregate, and 1h is sprayed every 11h, and the amount per 1kg regeneration aggregates spray mixed-culture medium is 2mL/min,
Intermittent shower 20 days, strengthened regeneration aggregate.According to standard GB/T25176-2010《Concrete and mortar regeneration aggregate》
The test microbes performance such as water absorption rate of intensifying regenerating aggregate before and after the processing, the results are shown in Table 2~3：
The granularity of intensifying regenerating aggregate in the embodiment of the present invention 2 of table 2
Intensifying regenerating aggregate is through the performance before and after microbiological treatment in the embodiment of the present invention 2 of table 3
It can be drawn by table 3, the water absorption rate of the intensifying regenerating aggregate after microbiological treatment is 2.7%, the suction of before processing
Water rate is 6.0%, and compared with before microbiological treatment, water absorption rate reduces 55.0%.
Embodiment 3
Regeneration mortar is prepared as reclaimed sand by the use of untreated regeneration aggregate in embodiment 2 and the regeneration aggregate after reinforcing,
Proportioning is shown in Table 4, investigates influence of the regeneration aggregate after microbial augmentation to mortar compression strength, the results are shown in Table 5.
Table 4 regenerates mortar mix ratio
Table 5 regenerates mortar compression strength
Embodiment 4
Regeneration mortar is prepared as reclaimed sand by the use of untreated regeneration aggregate in embodiment 2 and the regeneration aggregate after reinforcing,
Proportioning is shown in Table 6, investigates influence of the regeneration aggregate after microbial augmentation to mortar compression strength, the results are shown in Table 7.
Table 6 regenerates mortar mix ratio
Table 7 regenerates mortar compression strength
As seen from the above embodiment, using the present invention using bacillus B6 prepare high-performance regeneration aggregate method,
The water absorption rate and crush index value of obtained regeneration aggregate reduce, and after preparing regeneration mortar by regeneration aggregate, regenerate the anti-of mortar
Compressive Strength increase.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method that high-performance regeneration aggregate is prepared using bacillus B6, is comprised the following steps：
1) bacillus B6 is mixed with Mineralized Culture liquid, obtains mixed-culture medium；The Mineralized Culture liquid includes 10~45g/L
3- Cyclohexylamino -1- propane sulfonic acid, 4~12g/L sodium lactate, 1~3g/L sodium nitrate, 20~40mmol/L calcium chloride, 0.5
~1.5mmol/L magnesium sulfate, 0.08~0.18mmol/L potassium dihydrogen phosphate；The biological deposits numbering of the bacillus B6
For CGMCC No.13360；
2) the mixed-culture medium spray regeneration aggregate obtained using step 1), strengthened regeneration aggregate.
2. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 1, it is characterised in that described
The pH value of Mineralized Culture liquid is 10.1~10.9.
3. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 1, it is characterised in that described
In the mixed-culture medium that step 1) obtains, bacillus B6 bacterium number is 1 × 107~109cfu/L。
4. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 1, it is characterised in that step
1) the bacillus B6 is obtained through microbiological culture media culture；
The microbiological culture media includes beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions；The beef extract training
Base is supported using water as solvent, including 2.5~4.5g/L beef extract and 8~15g/L peptone；3- Cyclohexylaminos-the 1- third
Sulfonate buffer includes 120~170g/L 3- Cyclohexylamino -1- propane sulfonic acid using water as solvent.
5. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 4, it is characterised in that described
The pH value of 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions is 9~10.5.
6. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 4, it is characterised in that described
The volume ratio of beef extract culture medium and 3- Cyclohexylamino -1- propane sulfonic acid buffer solutions is (70~95):(10~20).
7. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 4, it is characterised in that described
The temperature of culture is 25~35 DEG C, and the time of the culture is 8~48h.
8. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 4, it is characterised in that described
Culture is carried out under oscillating condition, and the frequency of the vibration is 120~180rpm.
9. preparing the method for high-performance regeneration aggregate using bacillus B6 according to claim 1~8 any one, it is special
Sign is that the mode of the step 2) spray is intermittent shower, 0.5~1.5h is sprayed every 10~12h, per 1kg regeneration aggregates
The amount for spraying mixed-culture medium is 1~3mL/min.
10. the method for high-performance regeneration aggregate is prepared using bacillus B6 according to claim 9, it is characterised in that institute
The total time for stating spray is 15~30d.
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