CN107737617B - A kind of high-temperature heating liquid-transfering gun and its application method - Google Patents
A kind of high-temperature heating liquid-transfering gun and its application method Download PDFInfo
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- CN107737617B CN107737617B CN201710928524.0A CN201710928524A CN107737617B CN 107737617 B CN107737617 B CN 107737617B CN 201710928524 A CN201710928524 A CN 201710928524A CN 107737617 B CN107737617 B CN 107737617B
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- liquid
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- pipette
- transfering gun
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of high-temperature heating liquid-transfering gun and its application methods, liquid-transfering gun includes liquid relief gun body, it is disposed with resistive heater, insulating layer, shell from inside to outside in the outside of the liquid-transfering gun body lower part pipette, exhaust passage is provided between the insulating layer and shell, the upper end of exhaust passage passes through the inner cavity of steam valve connection pipette upper end, the bottom of the shell be it is open, be provided with the Heat-insulation valve of spring axis connection in the lower end of the insulating layer.Compared with prior art, the beneficial effects of the present invention are: the present invention can be used for the liquid relief of Biochemistry Experiment high temperature liquid, can during liquid relief liquid with precise control temperature, guarantee that liquid heat does not scatter and disappear, and can avoid some liquid and crystallized because of temperature reduction, thus experimental error caused by reducing because of liquid relief.
Description
Technical field
The present invention relates to it is a kind of biology and chemistry accurate experimental facilities more particularly to a kind of high-temperature heating liquid-transfering gun and its
Application method.
Background technique
Liquid-transfering gun is one kind of pipettor, is usually used in that laboratory is a small amount of or micro liquid pipettes.It is simple compared with pipette etc.
There is higher accuracy for liquid shifting equipment, can significantly reduce experimental error.Especially it is higher to error requirements experiment and
Speech can only use liquid-transfering gun, and liquid-transfering gun can only be cooperated to use for some analytical equipments.There are also multi-pass on the market
The liquid-transfering gun in road can take multiple samples simultaneously.It is also bigger to operate more convenient but opposite experimental error, and for some analyses
Equipment is such as: high performance liquid chromatograph can only cooperate single channel liquid-transfering gun to use.Thus this kind of liquid-transfering gun cannot be commonly used for
Various Biochemistry Experiments experiment especially higher to precise requirements uses.Common liquid-transfering gun has different rule on the market
Lattice, the liquid-transfering gun of different size match different size of pipette tips, and pipette tips, which are mostly that plastic material is not recyclable, to be reused.
Though the shape of different manufacturer's productions is different, working principle and operating method are almost the same.Most importantly: existing shifting
Liquid rifle is all only applicable to the use of Normal Atmospheric Temperature Liquid liquid relief.And temperature has larger shadow for the liquid of the liquid condition especially condition of high temperature
It rings, and then influences the error of experiment.
A kind of Novel pipettor disclosed in 204107526 U of Chinese patent literature CN enables to lantern ring is firm to be stuck in
In pipettor main body, suction nozzle uses polytetrafluoroethylene (PTFE) or glass material, can also prevent pipettor and solution cross contamination.It is a kind of
Pipettor, including pipettor main body, pipettor main body one end are connected with ejection sleeve, the pipettor main body other end and adjusting knob,
Control knob is connected, and the ejection key of control ejection sleeve is additionally provided in pipettor main body, and the ejection sleeve is connected with soup stick, soup stick
On be connected with suction nozzle, pipettor main body is equipped with groove, is equipped with the protrusion cooperated with groove inside lantern ring.The utility model passes through recessed
Slot and male cooperation are stuck in pipettor main body so that lantern ring is firm, prevent lantern ring from falling off and pipettor is caused to pollute.This is practical
New green environment protection improves the contaminated drawback of pipettor suction nozzle.But it is only intended to Normal Atmospheric Temperature Liquid liquid liquid relief, cannot generally be answered
It is used for various laboratory liquid reliefs.
103586095 A of Chinese patent literature CN discloses a kind of Multi-channel liquid transfer device, which includes moving
The regulating device of liquid device and liquid-transfering gun spacing, the liquid-transfering device include liquid relief driving element and liquid-transfering gun, liquid relief driving
Element includes liquid relief linear motor, pushes top plate, mandril guide rod；Liquid relief linear motor is fixed on the uppermost motor branch of device
On fagging, top plate centre is pushed to be equipped with circular trough, both sides are equipped with guide thread hole, and bottom is equipped with rectangular channel, and width is slightly larger than shifting
The diameter of liquid piston, liquid-transfering gun include liquid relief piston, piston cap, spring, liquid relief gun barrel, rifle point adapter head；First spring is nested
On liquid relief piston, liquid relief piston and spring are located in the round cavity of liquid relief gun barrel.The device is adapted to different pitchs of holes
ELISA Plate and tissue culture plate, realize multichannel, high-throughput liquid relief.The invention can draw different liquid simultaneously, improve
Working efficiency.But it is troublesome in poeration, and it is not suitable for the use of single channel liquid relief, heat insulating ability difference is only applicable to Normal Atmospheric Temperature Liquid liquid relief.
102101065 A of Chinese patent literature CN discloses a kind of pipette device, the pipettor can in a short time into
The adjustment of row collection capacity.By make push rod rotate and move in the axial direction plunger adjust the pipettor of the volume of cylindrical portion dress
In setting, the speed increasing mechanism setting of the push rod is transferred in pipettor main body and the push rod after inputted rotation speedup will be made
Between.The device can adjust collection capacity in a short time.But principle is complicated, and is only applicable to Normal Atmospheric Temperature Liquid liquid relief, cannot be universal
It is used applied to laboratory liquid relief.
Summary of the invention
The object of the present invention is to provide a kind of high-temperature heating liquid-transfering gun and its application methods, for high in Biochemistry Experiment
The liquid relief of geothermal liquid, can during liquid relief liquid with precise control temperature, guarantee that liquid heat does not scatter and disappear, and can avoid
A little liquid are crystallized because of temperature reduction, thus experimental error caused by reducing because of liquid relief.
To achieve the above object, the present invention is implemented with the following technical solutions:
A kind of high-temperature heating liquid-transfering gun, including liquid relief gun body, the outside of the liquid-transfering gun body lower part pipette by
It is disposed with resistive heater, insulating layer, shell from inside to outside, is provided with exhaust passage between the insulating layer and shell,
The upper end of exhaust passage by the inner cavity of steam valve connection pipette upper end, the bottom of the shell be it is open, described
The lower end of insulating layer is provided with the Heat-insulation valve of spring axis connection.
The steam valve is mounted on the outer wall of pipette upper end inner cavity by spring shaft.
It further include thermocouple, outlet, the positive and negative polarities of connection resistive heater inside the outlet, outside connects
Temperature controller is connect, pipette temperature is detected inside the thermocouple, it is external to send detection signal to temperature controller.
The shell uses muscovite material, and the pipette is made of polytetrafluoroethylene material, is screwed onto pipette
The suction nozzle of bottom is made of the synthetic material of polytetrafluoroethylene (PTFE) and silica.
A kind of application method being heated at high temperature liquid-transfering gun, the specific method is as follows:
1) press the control button of liquid relief gun body upper end downwards, suction nozzle opens downwards Heat-insulation valve, protrude into liquid into
Row imbibition operation；
3) after the completion of imbibition, pipette is retracted, and Heat-insulation valve is automatically closed under the action of spring shaft；
4) during liquid relief, temperature signal is simultaneously transferred to temperature controller by thermocouple detection pipette temperature, by temperature control
Instrument controls resistive heater according to the fluid temperature standard value of setting and pipette is heated or kept the temperature；
5) when pipette interior has extra steam generation, steam is run up, and the air pressure being gradually increased is by steam valve
Door opens, and shell is discharged downwards by the exhaust pipe on the outside of insulating layer in high-temperature steam.
Compared with prior art, the beneficial effects of the present invention are:
1) present invention can be used for the liquid relief of Biochemistry Experiment high temperature liquid, can accurately control during liquid relief
Fluid temperature guarantees that liquid heat does not scatter and disappear, and can avoid some liquid and crystallize because of temperature reduction, to reduce because liquid relief is made
At experimental error.
2) present invention can real-time detection temperature, fluid temperature in artificial accurate control liquid-transfering gun, and then Control Assay liquid
Temperature, improve precision, reduce experiment sampling bring error.
3) pipette upper end connects steam valve, the steam valve when there is excessive gas to generate pressure in change liquid-transfering gun gun body
Door is jacked up, and steam excludes outside gun body automatically by exhaust pipe, and when inside and outside pressure is consistent, steam valve can be automatically closed, and be made
It is easy to operate with safe and reliable.
4) present invention is provided with the Heat-insulation valve of insulating layer and bottom, can reduce energy consumption.
5) present invention uses the thermal insulation material of corrosion-and high-temp-resistant, long service life；Meanwhile suction nozzle and pipette are screw thread
It connects replaceable.
Detailed description of the invention
Fig. 1 is a kind of overall structure diagram for being heated at high temperature liquid-transfering gun of the present invention；
Fig. 2 is structural schematic diagram when liquid-transfering gun does not take liquid (Heat-insulation valve closing)；
Fig. 3 is structural schematic diagram when liquid-transfering gun takes liquid (Heat-insulation valve opening)；
Fig. 4 is the A point enlarged drawing of Fig. 3；
Fig. 5 is the partial schematic diagram of pipette；
Fig. 6 is the structural schematic diagram of suction nozzle；
Fig. 7 is the structural schematic diagram of spring shaft.
In figure: 1- control button；2- volume display window；3- outlet；4- shell；5- liquid relief gun body；6- thermocouple；
7- conducting wire；8- temperature controller resistive heater interface；9- temperature controller thermocouple interface；10- temperature controller；11- steam valve；12- bullet
Spring axis；13- pipette；14- exhaust passage；15- resistive heater；16- insulating layer；17- suction nozzle；18- spring shaft；19- heat preservation
Valve；20- resistive heater cathode；21- resistive heater anode；22- circlip；23- axle sleeve；24- axis；25- valve plate.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawing, it should be noted that implementation of the invention be not limited to it is following
Embodiment.
As shown in figs 1 to 6, a kind of high-temperature heating liquid-transfering gun, including liquid relief gun body 5, under the liquid relief gun body 5
The outside of portion's pipette 13 is disposed with resistive heater 15, insulating layer 16, shell 4 from inside to outside, in the insulating layer 16
Exhaust passage 14 is provided between shell 4, the upper end of exhaust passage 14 passes through 11 connection pipette of steam valve, 13 upper end
Inner cavity, the bottom of the shell 4 be it is open, be provided with the heat preservation of the connection of spring shaft 18 in the lower end of the insulating layer 16
Valve 19.
The steam valve 11 is mounted on the outer wall of 13 upper end inner cavity of pipette by spring shaft 12.
Further include thermocouple 6, outlet 3, the positive and negative polarities of resistive heater 15 connected inside the outlet 3,
External connection temperature controller 10, the thermocouple 6 is inserted in insulating layer 16 internal detection 13 temperature of pipette, external to temperature controller
10 send detection signal.
The shell of the shell 4 and liquid relief gun body is all made of muscovite material, and the pipette 13 uses polytetrafluoroethyl-ne
Alkene material is made, and the suction nozzle 17 for being screwed onto 13 bottom of pipette is made of the synthetic material of polytetrafluoroethylene (PTFE) and silica.
Insulating layer 16 uses asbestos material, can effectively keep the temperature to pipette 13 and suction nozzle 17, while at the bottom of insulating layer 16
Heat-insulation valve 19 is arranged in portion, while playing insulation effect, effectively prevents heat loss, can reduce power consumption.
In addition, being equipped with exhaust passage 14 on the outside of insulating layer 16, the hot steam generated in pipette 13 can be discharged, play
The effect of security protection, while the process that hot steam is discharged downwards along 16 periphery of insulating layer can also play certain guarantor to internal layer
Temperature effect.
See that Fig. 7, axis 24 are fixed on liquid-transfering gun, circlip 22 is socketed on axis 24, valve plate 25 is connected to axis 24 by axle sleeve 23
On, valve plate 25 is controlled by the elastic force of circlip 22 and is rebounded automatically.The spring shaft 12 of steam valve 11 and the spring of Heat-insulation valve 19
18 structure of axis is identical with working principle.
The present invention is the technological improvement to existing liquid-transfering gun, needs to lengthen on spring original in liquid relief gun body 5, can make
Suction nozzle 17 is reached by Heat-insulation valve 19 achievees the purpose that imbibition outside gun body.
A kind of application method being heated at high temperature liquid-transfering gun, the specific method is as follows:
1) control button 1 of 5 upper end of liquid relief gun body is pressed downwards, and suction nozzle 17 opens downwards Heat-insulation valve 19, protrudes into liquid
Imbibition operation is carried out in body；
3) after the completion of imbibition, pipette 13 is retracted, and Heat-insulation valve 19 is automatically closed under the action of spring shaft 18；
4) during liquid relief, thermocouple 6 detects 13 temperature of pipette and temperature signal is transferred to temperature controller 10, by
Temperature controller 10 controls resistive heater 15 according to the fluid temperature standard value of setting and pipette 13 is heated or kept the temperature；
Temperature controller is provided with temperature display, micro computer can be equipped in temperature controller with the temperature of real-time display pipette 13,
The temperature detection signal of real-time reception thermocouple 6 sets the standard value of temperature control according to liquid sample temperature, controls resistive heater
15 power supply.Resistive heater 15 can also be heated or be kept the temperature by temperature controller 10 by manual control.
5) when having extra steam generation inside pipette 13, steam is run up, and the air pressure being gradually increased is by steam
Valve 11 opens, and shell 4 is discharged downwards by the exhaust pipe 14 in 16 outside of insulating layer in high-temperature steam.
Claims (5)
1. a kind of high-temperature heating liquid-transfering gun, including liquid relief gun body, which is characterized in that in the liquid-transfering gun body lower part pipette
Outside be disposed with resistive heater, insulating layer, shell from inside to outside, the row of being provided between the insulating layer and shell
Gas channel, the upper end of exhaust passage pass through the inner cavity of steam valve connection pipette upper end, the bottom of the shell be it is open,
The Heat-insulation valve of spring axis connection is provided in the lower end of the insulating layer.
2. a kind of high-temperature heating liquid-transfering gun according to claim 1, which is characterized in that the steam valve passes through spring shaft
It is mounted on the outer wall of pipette upper end inner cavity.
3. a kind of high-temperature heating liquid-transfering gun according to claim 1, which is characterized in that it further include thermocouple, outlet,
The positive and negative polarities of connection resistive heater inside the outlet, external connection temperature controller, thermocouple inside detection are inhaled
Liquid pipe temperature, it is external to send detection signal to temperature controller.
4. a kind of high-temperature heating liquid-transfering gun according to claim 1, which is characterized in that the shell uses white clouds base material
Matter, the pipette are made of polytetrafluoroethylene material, are screwed onto the suction nozzle of imbibition bottom of the tube by polytetrafluoroethylene (PTFE) and dioxy
The synthetic material of SiClx is made.
5. it is a kind of claim 1-4 its any one of as described in high-temperature heating liquid-transfering gun application method, which is characterized in that
The specific method is as follows:
1) control button of liquid relief gun body upper end is pressed downwards, and suction nozzle opens downwards Heat-insulation valve, protrudes into liquid and inhaled
Liquid operation；
3) after the completion of imbibition, pipette is retracted, and Heat-insulation valve is automatically closed under the action of spring shaft；
4) during liquid relief, temperature signal is simultaneously transferred to temperature controller by thermocouple detection pipette temperature, by temperature controller root
Pipette is heated or kept the temperature according to the fluid temperature standard value control resistive heater of setting；
5) when pipette interior has extra steam generation, steam is run up, and the air pressure being gradually increased is by steam valve top
It opens, shell is discharged downwards by the exhaust pipe on the outside of insulating layer in high-temperature steam.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710928524.0A CN107737617B (en) | 2017-10-09 | 2017-10-09 | A kind of high-temperature heating liquid-transfering gun and its application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710928524.0A CN107737617B (en) | 2017-10-09 | 2017-10-09 | A kind of high-temperature heating liquid-transfering gun and its application method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107737617A CN107737617A (en) | 2018-02-27 |
| CN107737617B true CN107737617B (en) | 2019-10-25 |
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| CN201710928524.0A Active CN107737617B (en) | 2017-10-09 | 2017-10-09 | A kind of high-temperature heating liquid-transfering gun and its application method |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110227567A (en) * | 2019-06-19 | 2019-09-13 | 武汉科技大学 | A kind of numerical control high temperature liquid-transfering gun |
| CN112816257A (en) * | 2020-12-14 | 2021-05-18 | 武汉光谷航天三江激光产业技术研究院有限公司 | Micro liquid transfer device and method for precisely transferring alkali metal elements |
| CN114753985A (en) * | 2022-04-22 | 2022-07-15 | 深圳市恒永达科技股份有限公司 | Self-adaptive air type liquid transfer pump, high-precision liquid transfer method and storage medium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5287758A (en) * | 1991-01-26 | 1994-02-22 | Behringwerke Ag | Temperature controlled pipette tube |
| EP2145949A1 (en) * | 2007-04-06 | 2010-01-20 | Universal Bio Research Co., Ltd. | Temperature control device, and temperature control method |
| CN103521281A (en) * | 2013-10-23 | 2014-01-22 | 中国科学院声学研究所 | Temperature control pipetting device |
| CN206535568U (en) * | 2017-03-15 | 2017-10-03 | 福建中凯检测技术有限公司 | A kind of vacuum insulation wall thermometric takes liquid rifle |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3110056B2 (en) * | 1991-01-23 | 2000-11-20 | アークレイ株式会社 | Reagent heating method |
| EP1859868A1 (en) * | 2006-05-23 | 2007-11-28 | F.Hoffmann-La Roche Ag | Heatable pipette |
-
2017
- 2017-10-09 CN CN201710928524.0A patent/CN107737617B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5287758A (en) * | 1991-01-26 | 1994-02-22 | Behringwerke Ag | Temperature controlled pipette tube |
| EP2145949A1 (en) * | 2007-04-06 | 2010-01-20 | Universal Bio Research Co., Ltd. | Temperature control device, and temperature control method |
| CN103521281A (en) * | 2013-10-23 | 2014-01-22 | 中国科学院声学研究所 | Temperature control pipetting device |
| CN206535568U (en) * | 2017-03-15 | 2017-10-03 | 福建中凯检测技术有限公司 | A kind of vacuum insulation wall thermometric takes liquid rifle |
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| CN107737617A (en) | 2018-02-27 |
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