CN107737194A - Bactericide, preparation method and its application method for giant salamander sterilization - Google Patents
Bactericide, preparation method and its application method for giant salamander sterilization Download PDFInfo
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- CN107737194A CN107737194A CN201711199957.3A CN201711199957A CN107737194A CN 107737194 A CN107737194 A CN 107737194A CN 201711199957 A CN201711199957 A CN 201711199957A CN 107737194 A CN107737194 A CN 107737194A
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Abstract
A kind of bactericide for giant salamander sterilization, according to mass fraction, it is made up of following component:100 parts of Radix Isatidis, 5 15 parts of levisticum, 15 25 parts of Negundo Chastetree Leaf, 5 15 parts of Morinda officinalis, 45 60 parts of teasel root, 5 15 parts of motherwort, 5 15 parts of dandelion, 3 10 parts of gynostemma pentaphylla, 5 15 parts of the root of herbaceous peony, 10 20 parts of Chinese prickly ash, 10 20 parts of Qingqian Willow leaf, 25 35 parts of honeysuckle, 5 15 parts of polygala, 5 15 parts of folium cortex eucommiae, 3 10 parts of Bupleurum Chinese, 25 35 parts of argy wormwood, 25 35 parts of Chinese Fevervine Herb, 10 20 parts of wintercherry fruit or calyx, 3 10 parts of shepherd's purse, 5 10 parts of mulberry leaf.Using the bactericide of the present invention, the giant salamander is handled, sick probability can be reduced.
Description
Technical field
The invention belongs to giant salamander cultural technique field, and in particular to a kind of bactericide sterilized for the giant salamander, prepare
Method and its application method.
Background technology
Giant salamander is subordinate to Yin Sai salamanders section, giant salamander category, is amphibian existing maximum and most precious in the world；It is cried
The sob of acoustic image baby, therefore people are called its " giant salamander "；The giant salamander is commonly called as " big Chinese pepper fish ", and coming from its body has Chinese pepper taste
Road；The giant salamander is the transitional type for representing fish and reptiles, has the title of ancient " living fossil ", while doll's meat quality of fish is pure white fresh
It is tender, fertilizer and oiliness, taste specially fresh, containing abundant nutriment, be described as in water " living person's ginseng "；Wild doll's fish resource has been on the point of
Face extinction, it is to recover giant salamander natural resources to carry out artificial breeding scale, and the only way to satisfy social needs, the giant salamander is certainly
Due to the influence of extraneous many factors in right environment, its survival rate is not high, and this is the reason for giant salamander can not accrue quickly；
Under Artificial feeding conditions, it is difficult to which the situation of natural mating spawning occurs.
Because all especially high in the market of the ornamental value and edibility of the giant salamander is especially big to the demand of the giant salamander, people
Begin one's study the large-scale artificial farming giant salamander, and when the artificial farming giant salamander is carried out, a variety of methods are employed,
But survival rate is not still high.
A variety of diseases can occur during the giant salamander is cultivated, it is necessary to periodically be sterilized to giant salamander body, therefore,
It is necessary to develop a kind of bactericide for giant salamander sterilization, effect is good, pollution-free.
The content of the invention
An object of the present invention is to overcome the above-mentioned deficiency of prior art and provide a kind of killing for giant salamander sterilization
Microbial inoculum, red skin disease can be prevented and treated after use.
The second object of the present invention is the preparation method for providing the above-mentioned bactericide for giant salamander sterilization.
The third object of the present invention is the application method for providing the above-mentioned bactericide for giant salamander sterilization.
The technical scheme is that：A kind of bactericide for giant salamander sterilization, in terms of weight fraction, by following component
It is made：100 parts of Radix Isatidis, levisticum 5-15 parts, Negundo Chastetree Leaf 15-25 parts, Morinda officinalis 5-15 parts, teasel root 45-60 parts, motherwort 5-15
Part, dandelion 5-15 parts, gynostemma pentaphylla 3-10 parts, root of herbaceous peony 5-15 parts, Chinese prickly ash 10-20 parts, Qingqian Willow leaf 10-20 parts, honeysuckle 25-
35 parts, polygala 5-15 parts, folium cortex eucommiae 5-15 parts, Bupleurum Chinese 3-10 parts, argy wormwood 25-35 parts, Chinese Fevervine Herb 25-35 parts, wintercherry fruit or calyx 10-
20 parts, shepherd's purse 3-10 parts, mulberry leaf 5-10 parts.
The preferred 7-10 parts of wherein described levisticum, the preferred 17-22 parts of described Negundo Chastetree Leaf.
The preferred 9-12 parts of wherein described Morinda officinalis, the preferred 50-60 parts of described teasel root, the preferred 9-12 of described motherwort
Part.
The preferred 9-12 parts of wherein described dandelion, the preferred 5-9 parts of described gynostemma pentaphylla, the preferred 9-12 of the described root of herbaceous peony
Part.
The preferred 12-17 parts of wherein described Chinese prickly ash, the preferred 12-17 parts of described Qingqian Willow leaf, described honeysuckle are excellent
Select 27-32 parts.
The preferred 9-12 parts of wherein described polygala, the preferred 5-10 parts of described folium cortex eucommiae, the preferred 5-9 of described Bupleurum Chinese
Part.
The preferred 27-33 parts of wherein described argy wormwood, the preferred 27-32 parts of described Chinese Fevervine Herb, described wintercherry fruit or calyx are preferred
12-17 parts.
The preferred 5-9 parts of wherein described shepherd's purse, the preferred 4-8 parts of described mulberry leaf.
The preparation method of bactericide of the present invention for giant salamander sterilization, in turn includes the following steps：
（1）Raw material is mixed after crushing, sieving respectively, obtains compound；
（2）Add mixture into 5-10 times and measure water, be heated to 90-100 DEG C of decoction 2-4h；
（3）Filtering, filtrate are collected, and 95% ethanol water is measured in 5-10 times of filter residue addition, are heated to 50-70 DEG C of decoction 2-4h；
（4）Filtering, merging filtrate, filtrate are concentrated into no alcohol taste, produce the bactericide for giant salamander sterilization.
The application method of bactericide of the present invention for giant salamander sterilization is as follows：
Bactericide for giant salamander sterilization mixes with 100 times of amount water to carry out adult and puts advance in a suitable place to breed Ji Wei sterilization microbial inoculum, the giant salamander
Row dermal application sterilizes once, and later each two moon sterilization is once.
The beneficial effects of the present invention are：
The giant salamander is handled using the bactericide of the present invention, can be carried out disinfection sterilization to its skin, and red skin can be prevented and treated after use
Disease.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.
Table 1, table 2, the Example formulations for pharmaceutical composition of the present invention (in units of g).
Table 1
。
Table 2
。
It is used for the preparation method of the bactericide of giant salamander sterilization described in embodiment, comparative example, in turn includes the following steps：
（1）Raw material is mixed after crushing, sieving respectively, obtains compound；
（2）7 times of weight waters are added mixture into, are heated to 100 DEG C of decoction 3h；
（3）Filtering, filtrate are collected, and filter residue adds the ethanol water of 7 times of weight 95%, are heated to 60 DEG C of decoction 3h；
（4）Filtering, merging filtrate, filtrate are concentrated into no alcohol taste, produce the bactericide for giant salamander sterilization.
By it is above-mentioned for the giant salamander sterilization bactericide with 100 times amount water mix Ji Wei sterilize microbial inoculum, the giant salamander progress into
Body carries out dermal application sterilization once before putting in a suitable place to breed, later each two moon sterilization is once.
Experiment（One）：
The bactericide for being used for giant salamander sterilization obtained by embodiment 1-10, comparative example 1-2 is tested, randomly selects 260
The giant salamander, stochastic averagina are divided into 13 groups, and test group is handled by the way of embodiment 1-10, comparative example 1-2, and control group is conventional
Bactericide sterilizes, i.e. the rough gentian gentian violet of 1% concentration disinfects, and its method is that the ratio of medicine and water is 1: 100, and remaining is fed
Condition is identical with test group, and result of the test is shown in Table 3.
Table 3 is used for the test result of the bactericide of giant salamander sterilization
。
Claims (10)
1. a kind of bactericide for giant salamander sterilization, according to mass fraction, it is made up of following component:100 parts of Radix Isatidis, solely
Work 5-15 parts, Negundo Chastetree Leaf 15-25 parts, Morinda officinalis 5-15 parts, teasel root 45-60 parts, motherwort 5-15 parts, dandelion 5-15 parts, strand
The blue 3-10 parts of stock, root of herbaceous peony 5-15 parts, Chinese prickly ash 10-20 parts, Qingqian Willow leaf 10-20 parts, honeysuckle 25-35 parts, polygala 5-15 parts, Du
Zhong Ye 5-15 parts, Bupleurum Chinese 3-10 parts, argy wormwood 25-35 parts, Chinese Fevervine Herb 25-35 parts, wintercherry fruit or calyx 10-20 parts, shepherd's purse 3-10 parts, mulberry
Leaf 5-10 parts.
2. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described levisticum is 7-10 parts,
Described Negundo Chastetree Leaf is 17-22 parts.
3. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described Morinda officinalis is 9-12
Part, described teasel root is 50-60 parts, and described motherwort is 9-12 parts.
4. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described dandelion is 9-12
Part, described gynostemma pentaphylla is 5-9 parts, and the described root of herbaceous peony is 9-12 parts.
5. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described Chinese prickly ash is 12-17
Part, described Qingqian Willow leaf is 12-17 parts, and described honeysuckle is 27-32 parts.
6. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described polygala is 9-12 parts,
Described folium cortex eucommiae is 5-10 parts, and described Bupleurum Chinese is 5-9 parts.
7. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described argy wormwood is 27-33
Part, described Chinese Fevervine Herb is 27-32 parts, and described wintercherry fruit or calyx is 12-17 parts.
8. the bactericide according to claim 1 for giant salamander sterilization, it is characterised in that described shepherd's purse is 5-9 parts,
Described mulberry leaf are 4-8 parts.
9. it is used for the preparation method of the bactericide of giant salamander sterilization according to claim 1, it is characterised in that include successively such as
Lower step：
（1）Raw material is mixed after crushing, sieving respectively, obtains compound；
（2）5-10 times of weight water is added mixture into, is heated to 90-100 DEG C of decoction 2-4h；
（3）Filtering, filtrate are collected, and filter residue adds the ethanol water of 5-10 times of weight 95%, are heated to 50-70 DEG C of decoction 2-
4h ；
（4）Filtering, merging filtrate, filtrate are concentrated into no alcohol taste, produce the bactericide for giant salamander sterilization.
10. it is used for the application method of the bactericide of giant salamander sterilization according to claim 1, it is characterised in that include successively such as
Lower step：Bactericide for giant salamander sterilization is mixed with 100 times of weight amount water Ji Wei sterilization microbial inoculum, and the giant salamander carries out adult and put
Dermal application sterilization is carried out before supporting once, later each two moon sterilization is once.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711199957.3A CN107737194A (en) | 2017-11-27 | 2017-11-27 | Bactericide, preparation method and its application method for giant salamander sterilization |
Applications Claiming Priority (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110840987A (en) * | 2019-11-12 | 2020-02-28 | 衡东县新旺种养农民专业合作社 | Bactericide for sterilizing giant salamanders and preparation method thereof |
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| CN101785488A (en) * | 2010-03-19 | 2010-07-28 | 贵定锦江娃娃鱼开发有限公司 | Traditional Chinese medicine disinfectant of giant salamander culture pond |
| CN104161875A (en) * | 2013-05-20 | 2014-11-26 | 天津庚发水产养殖有限公司 | Preparation for prevention and treatment of bacterial diseases of freshwater fish and preparation method thereof |
| CN105727236A (en) * | 2016-03-30 | 2016-07-06 | 青岛海之星生物科技有限公司 | Powder for treating red skin disease of osphronemus goramy and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101785488A (en) * | 2010-03-19 | 2010-07-28 | 贵定锦江娃娃鱼开发有限公司 | Traditional Chinese medicine disinfectant of giant salamander culture pond |
| CN104161875A (en) * | 2013-05-20 | 2014-11-26 | 天津庚发水产养殖有限公司 | Preparation for prevention and treatment of bacterial diseases of freshwater fish and preparation method thereof |
| CN105727236A (en) * | 2016-03-30 | 2016-07-06 | 青岛海之星生物科技有限公司 | Powder for treating red skin disease of osphronemus goramy and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110840987A (en) * | 2019-11-12 | 2020-02-28 | 衡东县新旺种养农民专业合作社 | Bactericide for sterilizing giant salamanders and preparation method thereof |
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