CN107727632A - 一种sers增强的可控链状组装体检测凝血酶的方法 - Google Patents
一种sers增强的可控链状组装体检测凝血酶的方法 Download PDFInfo
- Publication number
- CN107727632A CN107727632A CN201710722621.4A CN201710722621A CN107727632A CN 107727632 A CN107727632 A CN 107727632A CN 201710722621 A CN201710722621 A CN 201710722621A CN 107727632 A CN107727632 A CN 107727632A
- Authority
- CN
- China
- Prior art keywords
- nano
- particle
- fibrin ferment
- particles
- sers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 42
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 229950003499 fibrin Drugs 0.000 title claims abstract description 36
- 102000009123 Fibrin Human genes 0.000 title claims abstract description 35
- 108010073385 Fibrin Proteins 0.000 title claims abstract description 35
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 17
- 239000002105 nanoparticle Substances 0.000 claims abstract description 89
- 108091023037 Aptamer Proteins 0.000 claims abstract description 22
- 230000008836 DNA modification Effects 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 28
- 239000002245 particle Substances 0.000 claims description 21
- 108020004414 DNA Proteins 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- GOMLCFUVZKLQCO-HTLAMOOLSA-N thrombin aptamer Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@@H]2[C@H](O[C@H](C2)N2C(NC(=O)C(C)=C2)=O)COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2COP(O)(=O)O[C@H]2[C@H]([C@@H](O[C@@H]2CO)N2C3=C(C(NC(N)=N3)=O)N=C2)O)N2C3=C(C(NC(N)=N3)=O)N=C2)O)N2C3=C(C(NC(N)=N3)=O)N=C2)O)N2C3=C(C(NC(N)=N3)=O)N=C2)O)N2C3=C(C(NC(N)=N3)=O)N=C2)O)N2C3=C(C(NC(N)=N3)=O)N=C2)O)N2C3=C(C(NC(N)=N3)=O)N=C2)O)[C@@H](OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=C(C(NC(N)=N3)=O)N=C2)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=C(C(NC(N)=N3)=O)N=C2)O)C1 GOMLCFUVZKLQCO-HTLAMOOLSA-N 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 3
- 108010094028 Prothrombin Proteins 0.000 claims description 3
- 102100027378 Prothrombin Human genes 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- 230000003111 delayed effect Effects 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000004048 modification Effects 0.000 claims description 3
- 238000012986 modification Methods 0.000 claims description 3
- 229940039716 prothrombin Drugs 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- ZVAVRBUTFWLEBK-UHFFFAOYSA-N NC1=CC(=C(C=C1)S)C1=CC=CC=C1 Chemical group NC1=CC(=C(C=C1)S)C1=CC=CC=C1 ZVAVRBUTFWLEBK-UHFFFAOYSA-N 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 230000003139 buffering effect Effects 0.000 claims 1
- 238000001069 Raman spectroscopy Methods 0.000 abstract description 8
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 108090000190 Thrombin Proteins 0.000 abstract description 4
- 229960004072 thrombin Drugs 0.000 abstract description 4
- 210000002966 serum Anatomy 0.000 abstract description 3
- 239000012082 adaptor molecule Substances 0.000 abstract description 2
- 239000012491 analyte Substances 0.000 abstract 1
- 239000010931 gold Substances 0.000 description 16
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 3
- 229940038773 trisodium citrate Drugs 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000009514 concussion Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000002331 protein detection Methods 0.000 description 2
- 229910001961 silver nitrate Inorganic materials 0.000 description 2
- 229910000033 sodium borohydride Inorganic materials 0.000 description 2
- 239000012279 sodium borohydride Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000011258 core-shell material Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000004186 food analysis Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000006197 hydroboration reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710722621.4A CN107727632B (zh) | 2017-08-22 | 2017-08-22 | 一种sers增强的可控链状组装体检测凝血酶的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710722621.4A CN107727632B (zh) | 2017-08-22 | 2017-08-22 | 一种sers增强的可控链状组装体检测凝血酶的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107727632A true CN107727632A (zh) | 2018-02-23 |
CN107727632B CN107727632B (zh) | 2020-08-04 |
Family
ID=61205225
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710722621.4A Active CN107727632B (zh) | 2017-08-22 | 2017-08-22 | 一种sers增强的可控链状组装体检测凝血酶的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107727632B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108398419A (zh) * | 2018-03-30 | 2018-08-14 | 华南师范大学 | 一种利用竞争型的纳米传感器超灵敏检测凝血酶的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100048412A1 (en) * | 2006-05-03 | 2010-02-25 | The Regents Of The University Of California | Detection of protease and protease activity using a single nanoscrescent sers probe |
CN101672770A (zh) * | 2008-08-11 | 2010-03-17 | 中国科学院长春应用化学研究所 | 基于金纳米探针和核酸适配子无标记比色测定凝血酶的方法 |
US20100105053A1 (en) * | 2008-10-10 | 2010-04-29 | Hansang Cho | Aptamer based sensors and related methods and systems |
CN104614358A (zh) * | 2014-12-09 | 2015-05-13 | 临沂大学 | 一种基于纳米粒子信号探针的拉曼放大检测凝血酶的方法 |
CN106770163A (zh) * | 2016-12-19 | 2017-05-31 | 中国科学院长春光学精密机械与物理研究所 | 一种凝血酶的检测方法 |
-
2017
- 2017-08-22 CN CN201710722621.4A patent/CN107727632B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100048412A1 (en) * | 2006-05-03 | 2010-02-25 | The Regents Of The University Of California | Detection of protease and protease activity using a single nanoscrescent sers probe |
CN101672770A (zh) * | 2008-08-11 | 2010-03-17 | 中国科学院长春应用化学研究所 | 基于金纳米探针和核酸适配子无标记比色测定凝血酶的方法 |
US20100105053A1 (en) * | 2008-10-10 | 2010-04-29 | Hansang Cho | Aptamer based sensors and related methods and systems |
CN104614358A (zh) * | 2014-12-09 | 2015-05-13 | 临沂大学 | 一种基于纳米粒子信号探针的拉曼放大检测凝血酶的方法 |
CN106770163A (zh) * | 2016-12-19 | 2017-05-31 | 中国科学院长春光学精密机械与物理研究所 | 一种凝血酶的检测方法 |
Non-Patent Citations (2)
Title |
---|
CRISTINA OCAÑA 等: "Signal amplification forthrombinimpedimetricaptasensor:Sandwich protocolanduseofgold-streptavidinnanoparticles", 《BIOSENSORS ANDBIOELECTRONICS》 * |
JINCAN HE 等: "Aptamer Recognition Induced Target-Bridged Strategy for Proteins Detection Based on Magnetic Chitosan and Silver/Chitosan Nanoparticles Using Surface-Enhanced Raman Spectroscopy", 《ANALYTICAL CHEMISTRY》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108398419A (zh) * | 2018-03-30 | 2018-08-14 | 华南师范大学 | 一种利用竞争型的纳米传感器超灵敏检测凝血酶的方法 |
CN108398419B (zh) * | 2018-03-30 | 2020-06-30 | 华南师范大学 | 一种利用竞争型的纳米传感器超灵敏检测凝血酶的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN107727632B (zh) | 2020-08-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Tu et al. | Molecularly imprinted polymer-based plasmonic immunosandwich assay for fast and ultrasensitive determination of trace glycoproteins in complex samples | |
Molinero-Fernández et al. | Electrochemical microfluidic micromotors-based immunoassay for C-reactive protein determination in preterm neonatal samples with sepsis suspicion | |
Swierczewska et al. | High-sensitivity nanosensors for biomarker detection | |
CN101438142B (zh) | 快速磁生物传感器 | |
Ranzoni et al. | One-step homogeneous magnetic nanoparticle immunoassay for biomarker detection directly in blood plasma | |
Pahlow et al. | Bioanalytical application of surface‐and tip‐enhanced R aman spectroscopy | |
Moerland et al. | Rotating magnetic particles for lab-on-chip applications–a comprehensive review | |
Farshchi et al. | Microfluidic biosensing of circulating tumor cells (CTCs): Recent progress and challenges in efficient diagnosis of cancer | |
Jiang et al. | The simultaneous detection of free and total prostate antigen in serum samples with high sensitivity and specificity by using the dual-channel surface plasmon resonance | |
Zhu et al. | Hydrophobic plasmonic nanoacorn array for a label-free and uniform SERS-based biomolecular assay | |
Li et al. | A SERS nano-tag-based fiber-optic strategy for in situ immunoassay in unprocessed whole blood | |
CN108169303A (zh) | 基于金属有机框架材料作为信号探针的核酸适配体电化学传感器 | |
Wang et al. | Multiplexed gold nanorod array biochip for multi-sample analysis | |
Sun et al. | Investigation of the recognition interaction between glycated hemoglobin and its aptamer by using surface plasmon resonance | |
Cong et al. | Microfluidic droplet-SERS platform for single-cell cytokine analysis via a cell surface bioconjugation strategy | |
WO2009083856A2 (en) | Concentrated unbound magnetic particle assay for biosensors | |
Kabay et al. | Disposable electrochemical immunosensor for prostate cancer detection | |
Choi et al. | Nanomaterial-based in vitro analytical system for diagnosis and therapy in microfluidic device | |
US20120058548A1 (en) | Detection of biotargets using bioreceptor functionalized nanoparticles | |
Karimzadeh et al. | Bio-assay: The best alternative for conventional methods in detection of epidermal growth factor | |
Chen et al. | Colorimetric biosensing assays based on gold nanoparticles functionalized/combined with non-antibody recognition elements | |
Sun et al. | Force-coded strategy for the simultaneous detection of multiple tumor-related proteins | |
Xu et al. | A micro-chamber free digital biodetection method via the “sphere-labeled-sphere” strategy | |
CN107727632A (zh) | 一种sers增强的可控链状组装体检测凝血酶的方法 | |
Zu et al. | In situ tumor cells detection using nanotube-functionalized & microfluidic-controlling multiresonance optical fiber |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20200710 Address after: 710000 Shaanxi Xi'an economic and Technological Development Zone, Fengcheng four road, Ming Guang road, southeast corner of the new business building 1808 Applicant after: SHAANXI ZHUANYI INTELLECTUAL PROPERTY OPERATION Co.,Ltd. Address before: 310000 Xihu District Wen two road, Hangzhou, Zhejiang Province, No. 207 Applicant before: Yang Lei |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201231 Address after: No.1 xc1001-2, Nanmen Gongnong Road, Chongfu Town, Tongxiang City, Jiaxing City, Zhejiang Province Patentee after: JIAXING JINJING E-COMMERCE Co.,Ltd. Address before: 710000 Shaanxi Xi'an economic and Technological Development Zone, Fengcheng four road, Ming Guang road, southeast corner of the new business building 1808 Patentee before: SHAANXI ZHUANYI INTELLECTUAL PROPERTY OPERATION Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231107 Address after: 314200 west side of 3rd floor, building 6, 988 Xinxing 2nd Road, Pinghu Economic and Technological Development Zone, Jiaxing City, Zhejiang Province Patentee after: Jiaxing Qianchun Biotechnology Co.,Ltd. Address before: No.1 xc1001-2, Nanmen Gongnong Road, Chongfu Town, Tongxiang City, Jiaxing City, Zhejiang Province Patentee before: JIAXING JINJING E-COMMERCE Co.,Ltd. |
|
TR01 | Transfer of patent right |