CN107727610B - Transmission spectrum imager for water-containing transparent tissue - Google Patents
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- 230000005540 biological transmission Effects 0.000 claims abstract description 64
- 238000003384 imaging method Methods 0.000 claims abstract description 34
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention discloses a transmission spectrum imager for water-containing transparent tissues, which consists of an embedded main controller, a terahertz scanning imaging subsystem, a multispectral imaging subsystem, a sample chamber and a wireless local area network transceiver. The invention has the advantages that the illumination transmission imaging method of the supercontinuum uniform laser source and the terahertz scanning lens imaging method are provided, the structure, texture, density, transmittance and other organization characteristics of an important biological resource sample can be obtained by combining the standard transparent liquid sample correction, the transmission spectrum image containing various information can be obtained, and the traceability, identification and protection of important biological resources can be conveniently carried out by customs import and export inspection and quarantine departments.
Description
Technical Field
The invention relates to a transmission-type spectral imager, in particular to a standard transmission-type imaging instrument and a standard transmission-type imaging method for scanning a terahertz source by combining illumination of a supercontinuum uniform laser source and a single beam, which are suitable for imaging analysis of water-containing transparent tissues of animals and plants and belong to the field of photoelectric imaging.
Background
In the import and export field, all countries adopt strict control measures for import and export of important biological resources. For example, the animal and plant inspection and quarantine bureau of the U.S. department of agriculture regulates that the entry and exit of genetic materials such as livestock, hatching eggs, animal semen, blood samples, embryos, and excreta are prohibited. In recent years, animals and plants exported in China have increased year by year, but at present, a complete metadata base for origin identification, physical properties, genetic characterization and the like is not established. It is imperative to strengthen the protection of special strategic biological resources and the origin tracing research and develop non-contact detection systems of various genetic resource samples.
In the aspects of constructing physical properties and genetic characterization metadata databases of samples such as rare animal blood, urine, semen, leaves of plants soaked by lactic acid and the like, the multispectral image with high resolution can reflect the characteristics of the samples in various aspects such as structure, texture, transparency, form and the like, and is a feasible and efficient technical means.
The invention adopts a transmission spectrum imaging method of a super-continuum spectrum uniform laser source, and is used for obtaining visible and mid-infrared spectrum multispectral standard transmittance images of samples such as rare animal blood, urine, semen, leaves of plants soaked by lactic acid and the like; and a method for scanning the terahertz source by a single beam is adopted to obtain a standard transmittance image of the terahertz spectrum segment. The invention provides a comprehensive transmission spectrum imager for protecting rare animal and plant imports and exports under the support of two technologies, which can be used for building and screening physical properties of animal and plant resources and is convenient for a customs import and export inspection and quarantine department to trace source, identify and protect important biological resources.
Disclosure of Invention
The invention aims to provide a comprehensive transmission imaging instrument for standard supercontinuum imaging and scanning terahertz imaging, which can obtain standard transmittance images of visible, mid-infrared and terahertz spectral bands of important biological resource samples such as rare animal blood, urine, semen, leaves of plants soaked by lactic acid and the like, and is used for establishing a library, tracing, identifying and protecting important biological resources.
The invention is realized by the following steps:
the comprehensive spectrum imager provided by the invention comprises an embedded main controller, a terahertz scanning imaging subsystem, a multispectral imaging subsystem, a sample chamber and a wireless local area network transceiver;
the terahertz scanning imaging subsystem consists of a transmitting head, a receiving head, a two-dimensional electric platform and a scanning and switching controller; the transmitting head comprises a terahertz source and a terahertz transmitting lens; the receiving head comprises a terahertz receiving lens and a terahertz sensor; the transmitting head and the receiving head are coaxially arranged by taking an Ethertzian optical axis as an axis and are fixed on a two-dimensional electric platform; the two-dimensional electric platform can perform two-dimensional plane scanning under the control of the scanning and switching controller;
the multispectral imaging subsystem consists of a filter wheel, a filter wheel controller, a band-pass filter, a supercontinuum laser, an optical fiber coupling mirror, a light-splitting optical fiber, a light homogenizer, a secondary light homogenizer, a multispectral camera, a multispectral imaging lens and a total reflector; the filter wheel is provided with a plurality of band-pass filters, and can be sequentially cut into the laser optical axis under the control of the filter wheel controller;
the sample chamber is internally provided with a sample pool, a standard transparent liquid sample and a switcher, and the sample is inserted into a corresponding notch of the sample chamber after being filled into the sample pool; the switcher controls and switches the standard transparent liquid sample or the sample cell to be inserted into the sample chamber; the standard transparent liquid sample is consistent with the plane size of the sample pool;
the super-continuum spectrum laser emits super-continuum spectrum laser in a visible-to-mid-infrared spectrum band, the super-continuum spectrum laser is transmitted along a laser optical axis, the laser with a certain passband is output after passing through an optical fiber coupling mirror and a certain bandpass filter, the laser with the certain passband is coupled into one-to-many optical fibers, and one-to-many beam splitting is carried out; the multiple output optical fibers of one-to-multiple optical fibers are uniformly arranged and distributed on the plane of the light homogenizer; the plane of the light equalizer is a diffuse transmission plane, so that the transmitted light beams can obtain a light equalizing effect; the homogenized passband laser beam is reflected by a full reflector and then turns to a main optical axis, and then passes through a secondary light homogenizing plate, the light distribution emitted from the surface of the secondary light homogenizing plate is more uniform, and when the secondary light homogenizing plate is illuminated on a sample in the sample chamber, the transmitted light is imaged on a multispectral camera through a multispectral imaging lens along the main optical axis; the laser optical axis is coplanar with the main optical axis and is vertical to the main optical axis;
the terahertz source can emit electromagnetic waves in a terahertz waveband, and the electromagnetic waves can be converged on a target at a certain distance along a terahertz optical axis through the terahertz emitting lens; the scanning and switching controller can control the two-dimensional electric platform to perform two-dimensional plane scanning, and can also control the two-dimensional electric platform to drive the transmitting head and the receiving head to switch in and out the space range of the sample chamber, wherein the switching-in state is a working area and is a working state; the cutting-out is a non-working area, and the cutting-out is in a non-working state; when the terahertz transmission lens is in a working state, the terahertz transmission lens converges terahertz waves transmitted along a terahertz optical axis to a certain point on a sample; the two-dimensional electric platform performs two-dimensional plane scanning, and can realize terahertz wave focusing on all points of a sample plane; the terahertz waves transmitted from the sample terahertz wave focusing point are continuously transmitted along a terahertz optical axis, and are converged on a terahertz sensor to be received through a terahertz receiving lens; the main optical axis and the terahertz optical axis are coplanar and parallel;
host software in the embedded main controller can realize man-machine interaction of instruments, database construction, query, remote transmission, fusion, analysis and classification identification of image information; the input/output port control program can realize the control of a wireless local area network transceiver, a switcher, a filter wheel controller, a terahertz source, a scanning and switching controller, a supercontinuum laser, a multispectral camera and a terahertz sensor, receive an output image of the multispectral camera and a single-point output signal of the terahertz sensor, and is connected with an entry and exit department cloud system through a wireless local area network transceiver network to realize the uploading and downloading of a database and cloud inquiry;
the comprehensive transmission spectrum imaging method provided by the invention comprises the following steps:
(1) laser multispectral transmission calibration
The embedded main controller starts the scanning and switching controller to control the two-dimensional electric platform to drive the transmitting head and the receiving head to enter a non-working area, and at the moment, the terahertz scanning imaging subsystem is in a non-working state; the embedded main controller controls to start the supercontinuum laser and the multispectral camera, the switcher is started to cut the standard transparent liquid sample into the sample chamber, the filter wheel controller is started to cut the first band-pass filter into the laser optical axis, at the moment, the transmitted light of the first pass band of the standard transparent liquid sample is imaged to the multispectral camera, and then the multispectral camera transmits the standard transmitted image of the first pass band to the embedded main controller;
after the acquisition of the standard transmission image of the first pass band is finished, the embedded main controller starts the filter wheel controller to cut the second band pass filter into the laser optical axis, at the moment, the transmission light of the second pass band of the standard transparent liquid sample is imaged to the multi-spectral camera, and then the multi-spectral camera transmits the standard transmission image of the second pass band to the embedded main controller; thereby completing the acquisition of the standard transmission image of the second passband;
similarly, the acquisition of the laser multispectral standard transmission image of the transparent liquid sample of the third, fourth and.
(2) Terahertz scanning transmission calibration
The embedded main controller controls to close the super-continuum spectrum laser and the multi-spectrum camera; the embedded main controller controls to start the terahertz source, the terahertz sensor and the start scanning and switching controller, controls the two-dimensional electric platform to drive the transmitting head and the receiving head to enter a working area, and then the terahertz scanning imaging subsystem is in a working state; the scanning and switching controller controls the two-dimensional electric platform to drive the transmitting head and the receiving head to carry out two-dimensional plane scanning, the scanning range of the scanning and switching controller covers the plane of a standard transparent liquid sample, the terahertz sensor collects terahertz transmission wave signals point by point in the scanning process and transmits the terahertz transmission wave signals to the embedded main controller (note: the pixel size of a terahertz scanning image is consistent with the pixel size of a laser super-continuum spectrum image through the selection of two-dimensional scanning step length), and the embedded main controller is spliced to obtain a terahertz standard transmission image which is consistent with the pixel arrangement of the laser multispectral standard transmission image;
(3) laser multispectral transmission imaging
The embedded main controller controls the closing of the terahertz source and the terahertz sensor, starts the scanning and switching controller, controls the two-dimensional electric platform to drive the transmitting head and the receiving head to enter a non-working area, and controls the terahertz scanning imaging subsystem to be in a non-working state;
the embedded main controller starts the switcher to cut the standard transparent liquid sample into a sample chamber; after a certain rare animal and plant sample is loaded into the sample cell, the sample cell is inserted into a corresponding notch of the sample chamber by a switcher; the embedded main controller controls to start the supercontinuum laser and the multispectral camera, the embedded main controller starts the filter wheel controller to cut the first piece, the second piece, the third piece and the last piece of band-pass filter into a laser optical axis in sequence, and meanwhile, the multispectral camera acquires transmission images of the sample under uniform laser illumination of each pass band in sequence and transmits the transmission images to the embedded main controller;
(4) terahertz transmission scanning imaging
The embedded main controller controls to close the super-continuum spectrum laser and the multi-spectrum camera; the embedded main controller controls to start the terahertz source, the terahertz sensor and the start scanning and switching controller, controls the two-dimensional electric platform to drive the transmitting head and the receiving head to enter a working area, and then the terahertz scanning imaging subsystem is in a working state;
the scanning and switching controller controls the two-dimensional electric platform to drive the transmitting head and the receiving head to carry out two-dimensional plane scanning, the scanning range covers the plane where the sample pool is located, the terahertz sensor collects terahertz transmission wave signals point by point in the scanning process (note: at the moment, a sample is placed, so that the terahertz transmission wave signals are absorbed when the scanning points penetrate through the sample, and the transmission signals are the maximum values when the scanning points fall outside the sample area), the terahertz transmission wave signals are transmitted to the embedded main controller, and the embedded main controller carries out splicing processing to obtain a terahertz transmission image of the sample with the same pixel size as the terahertz standard transmission image;
(5) image calculation processing and library building
The embedded main controller divides each passband transmission image of the sample by each corresponding passband standard transmission image respectively, thereby obtaining each passband standard transmittance image (namely a supercontinuum standard transmittance image) of the sample; similarly, dividing the terahertz transmission image of the sample by the terahertz standard transmission image by the embedded main controller to obtain a terahertz standard transmittance image; converging the super-continuum spectrum of the rare animal and plant sample with the terahertz standard transmittance image and the information of the species, the producing area, the organization and the like of the terahertz standard transmittance image to construct a physical property resource database of the biological structure, the texture, the water-containing distribution and the like of the terahertz standard transmittance image, and transmitting the database information of the sample to a cloud system of an entry and exit department through a wireless local area network transceiver network; after a large amount of rare animal and plant sample databases are built based on the instrument, the instrument is used for detecting unknown samples, after supercontinuum and terahertz standard transmittance images are obtained, the unknown samples and the rare animal and plant sample databases are searched, compared and identified, and origin tracing, species identification and the like can be performed, so that important biological resource protection is effectively performed, and national biological safety is maintained.
The invention has the advantages that the illumination transmission imaging method of the supercontinuum uniform laser source and the terahertz scanning lens imaging method are provided, the structure, texture, density, transmittance and other organization characteristics of an important biological resource sample can be obtained by combining the standard transparent liquid sample correction, the transmission spectrum image containing various information can be obtained, and the traceability, identification and protection of important biological resources can be conveniently carried out by customs import and export inspection and quarantine departments.
Drawings
FIG. 1 is a schematic diagram of the system of the present invention, in which: 1-embedded host controller; 2-terahertz scanning imaging subsystem; 3-scan and switch controller; 4-a terahertz source; 5-the emitter head; 6-terahertz optical axis; 7-terahertz emission lens; 8-a sample chamber; 9-sample cell; 10-sample; 11-secondary light homogenizing plate; 12 — main optical axis; 13-a receiving head; 14-filter wheel; 15-Filter wheel controller; 16-band pass filter; 17-laser optic axis; 18-supercontinuum laser; 19-fiber coupled mirror; 20-one-to-many optical fibers; 21-dodging device; 22-multispectral camera; 23-multispectral imaging subsystem; 24-multispectral imaging lens; 25-total reflection mirror; 26-wireless local area network transceiver; 27-working area; 28-non-working area; 29-terahertz sensor; 30-two-dimensional motorized stage; 31-Standard clear liquid sample; 32-a switch; 33-terahertz receiving lens.
Detailed Description
The specific embodiment of the present invention is shown in fig. 1.
The comprehensive spectrum imager provided by the invention is composed of an embedded main controller 1, a terahertz scanning imaging subsystem 2, a multispectral imaging subsystem 23, a sample room 8 and a wireless local area network transceiver 26;
the terahertz scanning imaging subsystem 2 consists of a transmitting head 5, a receiving head 13, a two-dimensional electric platform 30 and a scanning and switching controller 3; the transmitting head 5 comprises a terahertz source 4 and a terahertz transmitting lens 7; the receiving head 13 comprises a terahertz receiving lens 33 and a terahertz sensor 29; the transmitting head 5 and the receiving head 13 are coaxially arranged by taking the Hertz optical axis 6 as an axis and are fixed on a two-dimensional electric platform 30; the two-dimensional electric platform 30 can do two-dimensional plane scanning under the control of the scanning and switching controller 3;
the multispectral imaging subsystem 23 is composed of a filter wheel 14, a filter wheel controller 15, a band-pass filter 16, a supercontinuum laser 18, a fiber coupling mirror 19, a one-to-many fiber 20, a light homogenizer 21, a secondary light homogenizing plate 11, a multispectral camera 22, a multispectral imaging lens 24 and a total reflection mirror 25; a plurality of band-pass filters 16 (6 in the embodiment, the 400-2500nm spectral band is divided into 6 passbands) are arranged on the filter wheel 14, and can be sequentially cut into the laser optical axis 17 under the control of the filter wheel controller 15;
a sample cell 9, a standard transparent liquid sample 31 and a switcher 32 are arranged in the sample chamber 8, and a sample 10 is inserted into a corresponding notch of the sample chamber 8 after being filled into the sample cell 9; the switch 32 controls to switch the standard transparent liquid sample 31 or the sample cell 9 to be inserted into the sample chamber; the standard transparent liquid sample 31 is consistent with the plane size of the sample pool 9;
a supercontinuum laser 18 (note: the spectrum range of the laser is 400-2500nm, the average power is 2W) emits supercontinuum laser in the visible-to-mid-infrared spectrum band, the laser is transmitted along the laser optical axis 17, the laser with a certain pass band is output after passing through an optical fiber coupling mirror 19 and a certain band-pass filter 16, the laser is coupled into a one-to-many optical fiber 20, and one-to-many beam splitting is carried out (note: the embodiment adopts a one-to-sixteen optical fiber); the multiple output optical fibers of the one-to-multiple optical fibers 20 are uniformly arranged and distributed on the plane of the light homogenizer 21; the plane of the light equalizer 21 is a diffuse transmission plane, so that the transmitted light beams can obtain a light equalizing effect; the passband laser beam after the light uniformization turns to the main optical axis 12 after being reflected by the total reflection mirror 25, and then passes through the secondary light uniformizing plate 11, the light distribution emitted from the surface is more uniform, when the passband laser beam is illuminated on the sample 10 of the sample chamber 8, the transmission light is imaged on the multispectral camera 22 through the multispectral imaging lens 24 along the main optical axis 12; the laser optical axis 17 is coplanar with the main optical axis 12, and the laser optical axis 17 is perpendicular to the main optical axis 12;
the terahertz source 4 can emit electromagnetic waves in a terahertz waveband, and the electromagnetic waves can be converged on a target at a certain distance along a terahertz optical axis 6 through a terahertz emission lens 7; the scanning and switching controller 3 can control the two-dimensional electric platform 30 to perform two-dimensional plane scanning, and can also control the two-dimensional electric platform to drive the transmitting head 5 and the receiving head 13 to switch in and out the space range of the sample chamber, wherein the working area 27 is formed when the sample chamber is switched in, and the working state is formed at the moment; the cutting-out time is a non-working area 28, and the cutting-out time is a non-working state; in a working state, the terahertz emitting lens 7 converges terahertz waves transmitted along the terahertz optical axis 6 to a certain point on the sample 10; the two-dimensional electric platform 30 performs two-dimensional plane scanning, and can realize terahertz wave focusing on all points on the plane of the sample 10; the terahertz waves transmitted from the terahertz wave focusing point of the sample 10 continue to be transmitted along the terahertz optical axis 6, pass through the terahertz receiving lens 33, and are converged on the terahertz sensor 29 to be received; the main optical axis 12 and the terahertz optical axis 6 are coplanar and parallel;
host software in the embedded main controller 1 can realize human-computer interaction of instruments, database construction, query, remote transmission, fusion, analysis and classification identification of image information; the input/output port control program can realize the control of the wireless local area network transceiver 26, the switcher 32, the filter wheel controller 15, the terahertz source 4, the scanning and switching controller 3, the supercontinuum laser 18, the multispectral camera 22 and the terahertz sensor 29, receive the output image of the multispectral camera 22 and the single-point output signal of the terahertz sensor 29, and are connected with the cloud system of the entry and exit department through the wireless local area network transceiver 26 to realize the uploading, downloading and cloud inquiry of the database;
the comprehensive transmission spectrum imaging method provided by the invention comprises the following steps:
(1) laser multispectral transmission calibration
The embedded main controller 1 starts the scanning and switching controller 3, controls the two-dimensional electric platform 30 to drive the transmitting head 5 and the receiving head 13 to enter the non-working area 28, and at the moment, the terahertz scanning imaging subsystem 2 is in a non-working state; the embedded main controller 1 controls to start the supercontinuum laser 18 and the multispectral camera 22, a switcher 32 is started to cut a standard transparent liquid sample 31 into a sample chamber, a filter wheel controller 15 is started to cut a first band-pass filter 16 into a laser optical axis 17, at the moment, transmission light of a first pass band of the standard transparent liquid sample 31 is imaged to the multispectral camera 22, and then the multispectral camera 22 transmits the first pass band standard transmission image to the embedded main controller 1;
after the acquisition of the standard transmission image of the first passband is completed, the embedded main controller 1 starts the filter wheel controller 15 to cut the second bandpass filter 16 into the laser optical axis 17, at this time, the transmission light of the second passband of the standard transparent liquid sample 31 is imaged to the multi-spectral camera 22, and then the multi-spectral camera 22 transmits the standard transmission image of the second passband to the embedded main controller 1; thereby completing the acquisition of the standard transmission image of the second passband;
similarly, the laser multi-spectral standard transmission image acquisition of the standard transparent liquid sample 31 is completed sequentially from the third, fourth and so on until the last pass band (note: 6 pass bands in this embodiment);
(2) terahertz scanning transmission calibration
The embedded main controller 1 controls the super-continuum spectrum laser 18 and the multispectral camera 22 to be closed; the embedded main controller 1 controls to start a terahertz source 4 (note: in the embodiment, a 2.5THz terahertz cascade laser), a terahertz sensor 29 and a start scanning and switching controller 3, controls a two-dimensional electric platform 30 to drive a transmitting head 5 and a receiving head 13 to enter a working area 27, and controls the terahertz scanning imaging subsystem 2 to be in a working state; the scanning and switching controller 3 controls the two-dimensional electric platform 30 to drive the transmitting head 5 and the receiving head 13 to carry out two-dimensional plane scanning, the scanning range of the two-dimensional electric platform covers the plane where the standard transparent liquid sample 31 is located, in the scanning process, the terahertz sensor 29 collects terahertz transmission wave signals point by point and transmits the terahertz transmission wave signals to the embedded main controller 1 (note that the pixel size of a terahertz scanning image is consistent with the pixel size of a laser supercontinuum image through the selection of two-dimensional scanning step length, the resolution of the image is 2560 × 1920 in the embodiment), and the embedded main controller 1 is spliced to obtain a terahertz standard transmission image which is consistent with the pixel arrangement of the laser multispectral standard transmission image;
(3) laser multispectral transmission imaging
The embedded main controller 1 controls the terahertz source 4 and the terahertz sensor 29 to be closed, the embedded main controller 1 starts the scanning and switching controller 3, and controls the two-dimensional electric platform 30 to drive the transmitting head 5 and the receiving head 13 to enter the non-working area 28, and at the moment, the terahertz scanning imaging subsystem 2 is in a non-working state;
the embedded main controller 1 starts the switch 32 to cut the standard transparent liquid sample 31 into a sample chamber; after a certain rare animal and plant sample 10 is loaded into the sample cell 9, the switch 32 inserts the sample cell 9 into a corresponding notch of the sample chamber 8; the embedded main controller 1 controls to start the supercontinuum laser 18 and the multispectral camera 22, the embedded main controller 1 starts the filter wheel controller 15 to cut the first plate, the second plate, the third plate and the fourth plate in sequence until the last band-pass filter 16 cuts into the laser optical axis 17, and meanwhile, the multispectral camera 22 sequentially obtains transmission images of the sample 10 under uniform laser illumination of each pass band and transmits the transmission images to the embedded main controller 1;
(4) terahertz transmission scanning imaging
The embedded main controller 1 controls the super-continuum spectrum laser 18 and the multispectral camera 22 to be closed; the embedded main controller 1 controls to start the terahertz source 4, the terahertz sensor 29 and the scanning and switching controller 3, controls the two-dimensional electric platform 30 to drive the transmitting head 5 and the receiving head 13 to enter the working area 27, and at the moment, the terahertz scanning imaging subsystem 2 is in a working state;
the scanning and switching controller 3 controls the two-dimensional electric platform 30 to drive the transmitting head 5 and the receiving head 13 to carry out two-dimensional plane scanning, the scanning range covers the plane of the sample pool 9, the terahertz sensor 29 collects terahertz transmission wave signals point by point in the scanning process (note: the sample 10 is placed at the moment, so that the terahertz transmission wave signals are absorbed when the scanning points penetrate through the sample, and the transmission signals are the maximum values when the scanning points fall outside the sample area), the terahertz transmission signals are transmitted to the embedded main controller 1, and the main controller embedded controller 1 is spliced to obtain the terahertz transmission image of the sample 10 with the same pixel size as the terahertz standard transmission image;
(5) image calculation processing and library building
The embedded main controller 1 divides each passband transmission image of the sample 10 by each corresponding passband standard transmission image, thereby obtaining each passband standard transmittance image (i.e. a supercontinuum standard transmittance image) of the sample 10; similarly, the embedded main controller 1 divides the terahertz transmission image of the sample 10 by the terahertz standard transmission image thereof to obtain a terahertz standard transmittance image; converging the super-continuum spectrum of the rare animal and plant sample 10 with the terahertz standard transmittance image and the information of the species, the production place, the organization and the like of the terahertz standard transmittance image to construct a physical property resource database of the biological structure, the texture, the water-containing distribution and the like of the terahertz standard transmittance image, and transmitting the database information of the sample 10 to a cloud system of an entry and exit department through a wireless local area network transceiver 26; after a large amount of rare animal and plant sample databases are built based on the instrument, the instrument is used for detecting unknown samples, after supercontinuum and terahertz standard transmittance images are obtained, the unknown samples and the rare animal and plant sample databases are searched, compared and identified, and origin tracing, species identification and the like can be performed, so that important biological resource protection is effectively performed, and national biological safety is maintained.
Claims (1)
1. A transmission spectrum imager for water-containing transparent tissues is composed of an embedded main controller (1), a terahertz scanning imaging subsystem (2), a multispectral imaging subsystem (23), a sample chamber (8) and a wireless local area network transceiver (26); the method is characterized in that:
the terahertz scanning imaging subsystem (2) consists of a transmitting head (5), a receiving head (13), a two-dimensional electric platform (30) and a scanning and switching controller (3); the transmitting head (5) comprises a terahertz source (4) and a terahertz transmitting lens (7); the receiving head (13) comprises a terahertz receiving lens (33) and a terahertz sensor (29); the transmitting head (5) and the receiving head (13) are coaxially arranged by taking an Ethertzian optical axis (6) as an axis and are fixed on a two-dimensional electric platform (30); the two-dimensional electric platform (30) performs two-dimensional plane scanning under the control of the scanning and switching controller (3);
the multispectral imaging subsystem (23) consists of a filter wheel (14), a filter wheel controller (15), a band-pass filter (16), a supercontinuum laser (18), a fiber coupling mirror (19), a light-splitting optical fiber (20), a light homogenizer (21), a secondary light homogenizing plate (11), a multispectral camera (22), a multispectral imaging lens (24) and a total reflection mirror (25); a plurality of band-pass filters (16) are arranged on the filter wheel (14), and are sequentially cut into the laser optical axis (17) under the control of the filter wheel controller (15);
a sample cell (9), a standard transparent liquid sample (31) and a switcher (32) are arranged in the sample chamber (8), and a sample (10) is inserted into a corresponding notch of the sample chamber (8) after being put into the sample cell (9); the switch (32) controls to switch the standard transparent liquid sample (31) or the sample pool (9) to be inserted into the sample chamber; the standard transparent liquid sample (31) is consistent with the plane size of the sample pool (9);
the supercontinuum laser (18) emits supercontinuum laser in a visible spectrum section to a middle infrared spectrum section, the supercontinuum laser is transmitted along a laser optical axis (17), and is output as laser of a certain passband after passing through an optical fiber coupling mirror (19) and a certain bandpass filter (16), and the laser is coupled into a one-to-many optical fiber (20) to perform one-to-many beam splitting; the multiple output optical fibers of the one-to-multiple optical fibers (20) are uniformly arranged and distributed on the plane of the light homogenizer (21); the plane of the light equalizer (21) is a diffuse transmission plane, so that the transmitted light beam obtains a light equalizing effect; the homogenized passband laser beam is reflected by a total reflection mirror (25) and then turns to a main optical axis (12), and then passes through a secondary light homogenizing plate (11), the light distribution emitted from the surface is more uniform, when the passband laser beam is illuminated on a sample (10) of a sample chamber (8), the transmitted light is imaged on a multispectral camera (22) through a multispectral imaging lens (24) along the main optical axis (12); the laser optical axis (17) is coplanar with the main optical axis (12), and the laser optical axis (17) is vertical to the main optical axis (12);
the terahertz source (4) emits an electromagnetic wave in a terahertz waveband, and the electromagnetic wave is converged on a target at a certain distance along a terahertz optical axis (6) through a terahertz emission lens (7); the scanning and switching controller (3) controls the two-dimensional electric platform (30) to perform two-dimensional plane scanning, and controls the two-dimensional electric platform to drive the transmitting head (5) and the receiving head (13) to switch in and out a space range of the sample chamber, wherein the space range is a working area (27) when the sample chamber is switched in and is in a working state; the cutting-out time is a non-working area (28), and the cutting-out time is a non-working state; in the working state, the terahertz transmitting lens (7) converges terahertz waves transmitted along the terahertz optical axis (6) to a certain point on the sample (10); the two-dimensional electric platform (30) performs two-dimensional plane scanning to realize terahertz wave focusing on all points of the plane of the sample (10); the terahertz waves transmitted from the terahertz wave focusing point of the sample (10) continue to be transmitted along the terahertz optical axis (6), pass through the terahertz receiving lens (33), and are converged on the terahertz sensor (29) to be received; the main optical axis (12) and the terahertz optical axis (6) are coplanar and parallel;
host software in the embedded main controller (1) realizes man-machine interaction of instruments, database construction, query, remote transmission, fusion, analysis and classification identification of image information; the input and output port control program of the terahertz sensor control system controls a wireless local area network transceiver (26), a switcher (32), a filter wheel controller (15), a terahertz source (4), a scanning and switching controller (3), a supercontinuum laser (18), a multispectral camera (22) and a terahertz sensor (29), receives an output image of the multispectral camera (22) and a single-point output signal of the terahertz sensor (29), and is connected with an entry department cloud system through the wireless local area network transceiver (26) in a network manner to realize uploading and downloading of a database and cloud inquiry.
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