CN107884359B - Standard reflection type super-continuum spectrum imaging method - Google Patents
Standard reflection type super-continuum spectrum imaging method Download PDFInfo
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Abstract
The invention discloses a standard reflection type supercontinuum imaging method which is realized on the basis of a standard reflection type supercontinuum imager and comprises five steps of laser multispectral imaging calibration, terahertz scanning imaging calibration, laser multispectral imaging, terahertz scanning imaging, image post-processing and the like. The active illumination imaging mode eliminates the influence of ambient light, obtains visible light, infrared and terahertz multi-passband standard reflectivity images only related to the tissue characteristics of the sample, and is convenient for a customs import and export inspection and quarantine department to trace the source, identify and protect important biological resources.
Description
Technical Field
The invention relates to a super-continuum spectrum imaging method, in particular to a standard reflection type super-continuum spectrum imaging method adopting a super-continuum spectrum dodging laser source and a single beam scanning terahertz source, which is suitable for building and screening important biological resources such as rare animal feathers and skin and belongs to the field of photoelectric imaging.
Background
In the import and export field, all countries adopt strict control measures for import and export of important biological resources. For example, the animal and plant inspection and quarantine bureau of the U.S. department of agriculture regulates that the entry and exit of genetic materials such as livestock, hatching eggs, animal semen, blood samples, embryos, and excreta are prohibited. In recent years, animals and plants exported in China have increased year by year, but at present, a complete metadata base for origin identification, physical properties, genetic characterization and the like is not established. It is imperative to strengthen the protection of special strategic biological resources and the origin tracing research and develop non-contact detection systems of various genetic resource samples.
In the aspects of physical properties of important biological resources such as rare animal feathers, hide and the like and the construction of a genetic characterization metadata database, the multispectral image with high resolution can reflect the characteristics of texture, optics, water content, form and the like, and is a feasible and efficient technical means.
The invention adopts a spectral imaging method of a super-continuum spectrum uniform laser source, and is used for acquiring visible and mid-infrared spectral band multispectral standard reflectivity images of animal resource samples such as rare animal feathers and leathers; and acquiring a standard reflectivity image of a terahertz spectrum section by adopting a method of scanning a terahertz source by a single beam. The invention provides a standard reflection type supercontinuum imager for rare animal protection under the support of two technologies, which can be used for building and screening animal resources and is convenient for a customs import and export inspection and quarantine department to trace source, identify and protect important biological resources.
Disclosure of Invention
The invention aims to provide a standard reflection type supercontinuum imaging method, which can obtain multispectral standard reflectivity images of visible, mid-infrared and terahertz spectral bands of animal resource samples such as rare animal feathers and leathers and the like and is used for building, tracing, identifying and protecting important biological resources.
The invention is realized by the following steps:
the standard reflection type supercontinuum imager provided by the invention comprises an embedded main controller, a terahertz scanning imaging subsystem, a multispectral imaging subsystem, a sample chamber and a wireless local area network transceiver;
the terahertz scanning imaging subsystem consists of a terahertz scanning galvanometer switching and rotating controller, a terahertz source, a scanning galvanometer, a terahertz lens, a terahertz spectroscope and a terahertz sensor;
the multispectral imaging subsystem consists of a filter wheel, a filter wheel controller, a band-pass filter, a supercontinuum laser, an optical fiber coupling mirror, a light-splitting and multiple-optical fiber, a light homogenizer, a multispectral camera, a multispectral imaging lens and a semi-transparent and semi-reflective mirror; the filter wheel is provided with a plurality of band-pass filters, and can be sequentially cut into the laser optical axis under the control of the filter wheel controller;
the sample chamber is internally provided with a sample clamp, a standard blackboard, a standard white board and a switcher, and a sample is inserted into a corresponding notch of the sample chamber after being loaded into the sample clamp; the switcher controls and switches the standard blackboard or the standard white board to be used as a substrate of the sample chamber;
the super-continuum spectrum laser emits super-continuum spectrum laser in a visible-to-mid-infrared spectrum band, the super-continuum spectrum laser is transmitted along a laser optical axis, the laser with a certain passband is output after passing through an optical fiber coupling mirror and a certain bandpass filter, the laser with the certain passband is coupled into one-to-many optical fibers, and one-to-many beam splitting is carried out; the multiple output optical fibers of one-to-multiple optical fibers are uniformly arranged and distributed on the plane of the light homogenizer; the plane of the light equalizer is a diffuse transmission plane, so that the transmitted light beams can obtain a light equalizing effect; the homogenized passband laser beam is reflected by the semi-transparent semi-reflecting mirror and then turns to a main optical axis, the uniform illumination is carried out on a sample in the sample chamber, the reflected light is transmitted along the main optical axis in the reverse direction, passes through the semi-transparent semi-reflecting mirror and is imaged on a multispectral camera through a multispectral imaging lens; the laser optical axis, the main optical axis and the terahertz emission optical axis are coplanar, and the laser optical axis is perpendicular to the main optical axis and parallel to the terahertz emission optical axis;
the terahertz source can emit electromagnetic waves in a terahertz waveband, penetrate through the terahertz spectroscope along a terahertz emission optical axis and can be converged on a target at a certain distance through the terahertz lens; the terahertz scanning galvanometer switching and rotating controller is used for controlling the scanning galvanometer, switching in and switching out the main optical axis, and when the main optical axis is switched out, the main optical axis is in a non-working state, and the scanning galvanometer is in a position of a switching-out state; when the terahertz wave is cut into a sample, the terahertz wave is transmitted along the terahertz emission optical axis, and is converged at a certain point on the sample; the rotation of the scanning galvanometer is controlled, so that the terahertz waves of all points of the sample plane in a scanning range can be focused; the terahertz echo reflected by the terahertz wave focusing point of the sample is reflected by the scanning galvanometer in the reverse direction, then transferred to the terahertz emission optical axis, reflected by the terahertz lens and the terahertz spectroscope, transferred to the terahertz receiving optical axis, and converged on the terahertz sensor to be received; the terahertz receiving optical axis, the main optical axis and the terahertz emitting optical axis are coplanar, and the terahertz receiving optical axis is parallel to the main optical axis and is vertical to the terahertz emitting optical axis;
host software in the embedded main controller can realize man-machine interaction of instruments, database construction, query, remote transmission, fusion, analysis and classification identification of image information; the input and output port control program can realize the control of a wireless local area network transceiver, a switcher, a filter wheel controller, a terahertz source, a terahertz scanning galvanometer switching and rotating controller, a super-continuum spectrum laser, a multispectral camera and a terahertz sensor, receive an output image of the multispectral camera and a single-point output signal of the terahertz sensor, and is connected with an entry department cloud system through a wireless local area network transceiver network to realize the uploading and downloading of a database and cloud inquiry;
the standard reflection type supercontinuum imaging method provided by the invention comprises the following steps:
(1) laser multispectral imaging calibration
The embedded main controller controls to start the supercontinuum laser and the multispectral camera, the switcher is started to cut the standard white board into a substrate serving as a sample chamber, the filter wheel controller is started to cut the first band-pass filter into a laser optical axis, reflected light of a first pass band of the standard white board is imaged to the multispectral camera at the moment, and then the multispectral camera transmits a standard white reflected image of the first pass band to the embedded main controller; the embedded main controller starts the switcher to cut the standard white board out of the sample chamber and cut the standard blackboard into the substrate serving as the sample chamber, reflected light of a first pass band of the standard blackboard is imaged to the multispectral camera at the moment, and then the multispectral camera transmits a standard black reflected image of the first pass band to the embedded main controller;
after the standard black-and-white reflection image of the first pass band is obtained, the embedded main controller starts the filter wheel controller to cut the second band-pass filter into the laser optical axis, starts the switcher to cut the standard blackboard into the sample chamber, and cuts the standard whiteboard into the substrate serving as the sample chamber, at the moment, the reflection light of the second pass band of the standard whiteboard is imaged to the multi-spectral camera, and then the multi-spectral camera transmits the standard white reflection image of the second pass band to the embedded main controller; then, the embedded main controller starts a switcher to cut the standard white board out of the sample chamber and cut the standard blackboard into the substrate serving as the sample chamber, reflected light of a second pass band of the standard blackboard is imaged to the multi-spectral camera at the moment, and then the multi-spectral camera transmits the standard black reflected image of the second pass band to the embedded main controller; thereby completing the acquisition of the standard black-and-white reflection image of the second passband;
similarly, completing the black-and-white reflection image acquisition of the third, fourth and.
(2) Terahertz scanning imaging calibration
The embedded main controller controls to close the super-continuum spectrum laser and the multi-spectrum camera; the embedded main controller starts the switcher to cut the standard blackboard into a sample chamber and cut the standard whiteboard into a substrate serving as the sample chamber; the embedded main controller controls to start the terahertz source and the terahertz sensor, starts the terahertz scanning galvanometer switching and the rotary controller to switch the scanning galvanometer into the main optical axis, and the scanning galvanometer is in a working state at the moment; the terahertz scanning galvanometer switching and rotating controller controls the scanning galvanometer to carry out two-axis scanning, the reflection of the terahertz scanning galvanometer covers the whole scanning range in Hertz waves, and in the scanning process, a terahertz sensor collects terahertz reflection echo signals of a standard white board point by point and transmits the terahertz reflection echo signals to the embedded main controller, and the embedded main controller processes the signals to obtain a standard terahertz reflection white image;
then, the embedded main controller starts a switcher to cut the standard white board out of the sample chamber and cut the standard blackboard into a substrate serving as the sample chamber; the terahertz scanning galvanometer switching and rotating controller controls the scanning galvanometer to carry out two-axis scanning, and in the scanning process, a terahertz sensor collects standard blackboard terahertz reflection echo signals point by point and transmits the signals to the embedded main controller, and the embedded main controller processes the signals to obtain a standard terahertz reflection black image;
(3) laser multispectral imaging
The embedded main controller controls to close the terahertz source and the terahertz sensor, starts the terahertz scanning galvanometer switching and rotates the controller to cut the scanning galvanometer out of a main optical axis, and the scanning galvanometer is in a non-working state at the moment;
after a feather or hide sample of a rare animal is put into a sample clip, the sample clip is inserted into a corresponding notch of a sample chamber; the embedded main controller controls to start the supercontinuum laser and the multispectral camera, the embedded main controller starts the filter wheel controller to cut the first plate, the second plate, the third plate and the last plate of the band-pass filter into the laser optical axis in sequence, and meanwhile, the multispectral camera acquires black bottom reflection images of the sample under uniform laser illumination of each pass band and with a standard blackboard as a background in sequence and transmits the black bottom reflection images to the embedded main controller;
(4) terahertz scanning imaging
The embedded main controller controls to close the super-continuum spectrum laser and the multi-spectrum camera; the embedded main controller controls to start the terahertz source and the terahertz sensor, starts the terahertz scanning galvanometer switching and the rotary controller to switch the scanning galvanometer into the main optical axis, and the scanning galvanometer is in a working state at the moment; the terahertz scanning galvanometer switching and rotating controller controls the scanning galvanometer to carry out two-axis scanning, in the scanning process, a terahertz sensor collects terahertz reflection echo signals of a sample with a standard blackboard as a background point by point, the terahertz reflection echo signals are transmitted to the embedded main controller, and the embedded main controller processes the signals to obtain a standard terahertz black bottom reflection image of the sample;
(5) image post-processing
The embedded main controller subtracts the corresponding passband standard black reflection image and standard terahertz reflection black image from the corresponding passband black bottom reflection image and terahertz black bottom reflection image of the sample respectively, and then divides the passband standard white reflection image and the standard terahertz reflection white image corresponding to the passband black bottom reflection image and terahertz black reflection image respectively, so as to obtain each passband standard reflectivity image and terahertz standard reflectivity image (namely supercontinuum standard reflectivity image) of the sample; the embedded main controller converges the supercontinuum standard reflectivity image of the rare animal feather or leather sample and the information of the species, the origin, the organization and the like of the rare animal feather or leather sample to construct a biological resource database of the rare animal feather or leather sample, and transmits the database information of the rare animal feather or leather sample to a cloud system of an entry-exit department through a wireless local area network transceiver network; after a large amount of rare animal feather or hide database is constructed based on the instrument, the instrument is used for detecting unknown samples, after supercontinuum standard reflectivity images are obtained, the unknown samples and the rare animal feather or hide database are searched, compared and identified, and origin tracing, species identification and the like can be carried out, so that important biological resource protection is effectively carried out, and national biological safety is maintained.
The active illumination imaging mode eliminates the influence of ambient light, obtains visible light, infrared and terahertz multi-passband standard reflectivity images only related to the tissue characteristics of the sample, and is convenient for a customs import and export inspection and quarantine department to trace the source, identify and protect important biological resources.
Drawings
FIG. 1 is a schematic diagram of the system of the present invention, in which: 1-embedded host controller; 2-terahertz scanning imaging subsystem; 3-terahertz scanning galvanometer switching and rotating controller; 4-a terahertz source; 5-cut-out state; 6-terahertz emission optical axis; 7-scanning galvanometer; 8-a sample chamber; 9-sample holder; 10-sample; 11-standard white board; 12 — main optical axis; 13-scanning range; 14-filter wheel; 15-Filter wheel controller; 16-band pass filter; 17-laser optic axis; 18-supercontinuum laser; 19-fiber coupled mirror; 20-one-to-many optical fibers; 21-dodging device; 22-multispectral camera; 23-multispectral imaging subsystem; 24-multispectral imaging lens; 25-half mirror; 26-wireless local area network transceiver; 27-terahertz lens; 28-terahertz spectroscope; 29-terahertz sensor; 30-terahertz receiving optical axis; 31-Standard blackboard; 32-switch.
Detailed Description
The specific embodiment of the present invention is shown in fig. 1.
The standard reflection type supercontinuum imager provided by the invention is composed of an embedded main controller 1, a terahertz scanning imaging subsystem 2, a multispectral imaging subsystem 23, a sample room 8 and a wireless local area network transceiver 26;
the terahertz scanning imaging subsystem 2 consists of a terahertz scanning galvanometer switching and rotating controller 3, a terahertz source 4, a scanning galvanometer 7, a terahertz lens 27, a terahertz spectroscope 28 and a terahertz sensor 29;
the multispectral imaging subsystem 23 is composed of a filter wheel 14, a filter wheel controller 15, a band-pass filter 16, a supercontinuum laser 18, a fiber coupling mirror 19, a one-to-many fiber 20, a light homogenizer 21, a multispectral camera 22, a multispectral imaging lens 24 and a half-mirror 25; a plurality of band-pass filters 16 (6 in the embodiment, the 400-2500nm spectral band is divided into 6 passbands) are arranged on the filter wheel 14, and can be sequentially cut into the laser optical axis 17 under the control of the filter wheel controller 15;
a sample clamp 9, a standard blackboard 31, a standard white board 11 and a switcher 32 are arranged in the sample chamber 8, and a sample 10 is inserted into a corresponding notch of the sample chamber 8 after being loaded into the sample clamp 9; the switch 32 controls to switch the standard blackboard 31 or the standard whiteboard 11 as the base of the sample chamber;
a supercontinuum laser 18 (note: the spectrum range of the laser is 400-2500nm, the average power is 2W) emits supercontinuum laser in the visible-to-mid-infrared spectrum band, the laser is transmitted along the laser optical axis 17, the laser with a certain pass band is output after passing through an optical fiber coupling mirror 19 and a certain band-pass filter 16, the laser is coupled into a one-to-many optical fiber 20, and one-to-many beam splitting is carried out (note: the embodiment adopts a one-to-sixteen optical fiber); the multiple output optical fibers of the one-to-multiple optical fibers 20 are uniformly arranged and distributed on the plane of the light homogenizer 21; the plane of the light equalizer 21 is a diffuse transmission plane, so that the transmitted light beams can obtain a light equalizing effect; the homogenized passband laser beams are reflected by the half mirror 25 and then turn to the main optical axis 12, and are uniformly illuminated on the sample 10 in the sample chamber 8, the reflected light is reversely transmitted along the main optical axis 12, passes through the half mirror 25, and is imaged on the multispectral camera 22 through the multispectral imaging lens 24; the laser optical axis 17, the main optical axis 12 and the terahertz emission optical axis 6 are coplanar, and the laser optical axis 17 is perpendicular to the main optical axis 12 and parallel to the terahertz emission optical axis 6;
the terahertz source 4 can emit an electromagnetic wave in a terahertz waveband, and the electromagnetic wave can penetrate through the terahertz spectroscope 28 along the terahertz emission optical axis 6 and can be converged on a target at a certain distance through the terahertz lens 27; the terahertz scanning galvanometer switching and rotating controller 3 is used for controlling the scanning galvanometer 7, switching in and switching out the main optical axis 12, and when the main optical axis is switched out, the main optical axis is in a non-working state, and the scanning galvanometer 7 is in a position of a switching-out state 5; when the cutting-in is in a working state, the scanning galvanometer 7 is positioned at the position of the main optical axis 12 and can rotate along two axes under the control of the terahertz scanning galvanometer switching and rotating controller 3, so that terahertz waves transmitted along the terahertz emission optical axis 6 are turned and converged at a certain point on the sample 10; the rotation of the scanning galvanometer 7 is controlled, so that the terahertz waves of all points of the plane of the sample 10 in the scanning range 13 can be focused; the terahertz echo reflected by the terahertz wave focusing point of the sample 10 is reflected by the scanning galvanometer 7 in the reverse direction, then transferred to the terahertz emission optical axis 6, then transferred to the terahertz receiving optical axis 30 after being reflected by the terahertz lens 27 and the terahertz spectroscope 28, and converged on the terahertz sensor 29 for receiving; the terahertz receiving optical axis 30, the main optical axis 12 and the terahertz emitting optical axis 6 are coplanar, and the terahertz receiving optical axis 30 is parallel to the main optical axis 12 and is perpendicular to the terahertz emitting optical axis 6;
host software in the embedded main controller 1 can realize human-computer interaction of instruments, database construction, query, remote transmission, fusion, analysis and classification identification of image information; the input/output port control program can realize the control of the wireless local area network transceiver 26, the switcher 32, the filter wheel controller 15, the terahertz source 4, the terahertz scanning galvanometer switching and rotating controller 3, the supercontinuum laser 18, the multispectral camera 22 and the terahertz sensor 29, receive the output image of the multispectral camera 22 and the single-point output signal of the terahertz sensor 29, and are connected with the cloud system of the entry and exit department through the wireless local area network transceiver 26 to realize the uploading and downloading of the database and the cloud inquiry;
the standard reflection type supercontinuum imaging method provided by the invention comprises the following steps:
(1) laser multispectral imaging calibration
The embedded main controller 1 controls to start the supercontinuum laser 18 and the multispectral camera 22, the switch 32 is started to cut the standard white board 11 into the substrate serving as the sample chamber, the filter wheel controller 15 is started to cut the first band-pass filter 16 into the laser optical axis 17, the reflected light of the first pass band of the standard white board 11 is imaged to the multispectral camera 22 at the moment, and then the multispectral camera 22 transmits the standard white reflected image of the first pass band to the embedded main controller 1; the embedded main controller 1 starts the switcher 32 to cut the standard white board 11 out of the sample chamber, and cuts the standard blackboard 31 into the substrate as the sample chamber, at this time, the reflected light of the first pass band of the standard blackboard 31 is imaged to the multispectral camera 22, and then the multispectral camera 22 transmits the standard black reflected image of the first pass band to the embedded main controller 1;
after the acquisition of the standard black-and-white reflection image of the first pass band is completed, the embedded main controller 1 starts the filter wheel controller 15 to cut the second band pass filter 16 into the laser optical axis 17, starts the switcher 32 to cut the standard blackboard 31 into the sample chamber, and cuts the standard whiteboard 11 into the substrate serving as the sample chamber, at this time, the reflection light of the second pass band of the standard whiteboard 11 is imaged to the multispectral camera 22, and then the multispectral camera 22 transmits the standard white reflection image of the second pass band to the embedded main controller 1; then, the embedded main controller 1 starts the switch 32 to cut the standard white board 11 out of the sample chamber, and cuts the standard blackboard 31 into the substrate as the sample chamber, at this time, the reflected light of the second pass band of the standard blackboard 31 is imaged to the multi-spectral camera 22, and then the multi-spectral camera 22 transmits the reflected image of the second pass band standard black to the embedded main controller 1; thereby completing the acquisition of the standard black-and-white reflection image of the second passband;
similarly, the black and white reflection image acquisition of the third, fourth, and.. until the last pass band (note: 6 pass bands in this embodiment) is completed in sequence;
(2) terahertz scanning imaging calibration
The embedded main controller 1 controls the super-continuum spectrum laser 18 and the multispectral camera 22 to be closed; the embedded main controller 1 starts a switcher 32 to cut the standard blackboard 31 into a sample chamber and cut the standard whiteboard 11 into a substrate serving as the sample chamber; the embedded main controller 1 controls to start a terahertz source 4 (note: in the embodiment, a 2.5THz terahertz cascade laser), a terahertz sensor 29, start a terahertz scanning galvanometer switching and rotating controller 3 to cut a scanning galvanometer 7 into a main optical axis 12, and at the moment, the scanning galvanometer 7 is in a working state; the terahertz scanning galvanometer switching and rotating controller 3 controls the scanning galvanometer 7 to carry out biaxial scanning, the reflection of the terahertz scanning galvanometer covers the whole scanning range 13 in Hertz waves, in the scanning process, a terahertz sensor 29 collects terahertz reflection echo signals of a standard white board 11 point by point and transmits the terahertz reflection echo signals to the embedded main controller 1 (note: the pixel size of a terahertz scanning image is consistent with the pixel size of a laser supercontinuum image through the selection of biaxial scanning step length, the resolution of the image in the embodiment is 2560 × 1920), and the embedded main controller 1 processes the terahertz reflection echo signals to obtain a standard terahertz reflection white image;
then, the embedded main controller 1 starts the switch 32 to cut the standard white board 11 into a sample chamber, and cuts the standard white board 31 into a substrate as the sample chamber; the terahertz scanning galvanometer switching and rotating controller 3 controls the scanning galvanometer 7 to carry out two-axis scanning, and in the scanning process, a terahertz sensor 29 collects terahertz reflection echo signals of a standard blackboard 31 point by point and transmits the terahertz reflection echo signals to the embedded main controller 1, and the embedded main controller 1 processes the signals to obtain a standard terahertz reflection black image;
(3) laser multispectral imaging
The embedded main controller 1 controls to close the terahertz source 4 and the terahertz sensor 29, starts the terahertz scanning galvanometer switching and rotating controller 3 to cut the scanning galvanometer 7 out of the main optical axis 12, and at the moment, the scanning galvanometer 7 is in a non-working state;
after a certain rare animal feather or leather sample 10 is put into a sample clamp 9, the sample is inserted into a corresponding notch of a sample chamber 8; the embedded main controller 1 controls to start the supercontinuum laser 18 and the multispectral camera 22, the embedded main controller 1 starts the filter wheel controller 15 to cut the first plate, the second plate, the third plate and the fourth plate in sequence until the last band-pass filter 16 cuts into the laser optical axis 17, and meanwhile, the multispectral camera 22 sequentially obtains a black bottom reflection image of the sample 10 under uniform laser illumination of each pass band and with the standard blackboard 31 as a background, and transmits the black bottom reflection image to the embedded main controller 1;
(4) terahertz scanning imaging
The embedded main controller 1 controls the super-continuum spectrum laser 18 and the multispectral camera 22 to be closed; the embedded main controller 1 controls to start the terahertz source 4 and the terahertz sensor 29, starts the terahertz scanning galvanometer switching and rotating controller 3 to cut the scanning galvanometer 7 into the main optical axis 12, and at the moment, the scanning galvanometer 7 is in a working state; the terahertz scanning galvanometer switching and rotating controller 3 controls the scanning galvanometer 7 to carry out two-axis scanning, in the scanning process, the terahertz sensor 29 collects terahertz reflection echo signals of the sample 10 with the standard blackboard 31 as the background point by point, transmits the terahertz reflection echo signals to the embedded main controller 1, and the embedded main controller 1 processes the signals to obtain a standard terahertz black bottom reflection image of the sample 10;
(5) image post-processing
The embedded main controller 1 subtracts the corresponding passband standard black reflection image and standard terahertz reflection black image from each passband black bottom reflection image and terahertz black bottom reflection image of the sample 10, and then divides the corresponding passband standard white reflection image and standard terahertz reflection white image to obtain each passband standard reflectance image and terahertz standard reflectance image (i.e. supercontinuum standard reflectance image) of the sample 10; the embedded main controller 1 converges the supercontinuum standard reflectivity image of the rare animal feather or leather sample 10 and the information of the species, the origin, the organization and the like of the rare animal feather or leather sample to construct a biological resource database of the rare animal feather or leather sample, and transmits the database information of the rare animal feather or leather sample 10 to a cloud system of an entry-exit department through a wireless local area network transceiver 26 network; after a large amount of rare animal feather or hide database is constructed based on the instrument, the instrument is used for detecting unknown samples, after supercontinuum standard reflectivity images are obtained, the unknown samples and the rare animal feather or hide database are searched, compared and identified, and origin tracing, species identification and the like can be carried out, so that important biological resource protection is effectively carried out, and national biological safety is maintained.
Claims (1)
1. A standard reflection type supercontinuum imaging method is realized on a standard reflection type supercontinuum imager, wherein the imager consists of an embedded main controller (1), a terahertz scanning imaging subsystem (2), a multispectral imaging subsystem (23), a sample room (8) and a wireless local area network transceiver (26); the standard reflection type supercontinuum imaging method is characterized by comprising the following steps:
1) laser multispectral imaging calibration
The embedded main controller controls and starts the supercontinuum laser and the multispectral camera, the switcher is started to cut the standard white board into a substrate serving as a sample chamber, the filter wheel controller is started to cut the first band-pass filter into a laser optical axis, reflected light of a first pass band of the standard white board is imaged to the multispectral camera at the moment, and then the multispectral camera transmits a standard white reflected image of the first pass band to the embedded main controller; the embedded main controller starts the switcher to cut the standard white board out of the sample chamber and cut the standard blackboard into the substrate of the sample chamber, at the moment, reflected light of a first pass band of the standard blackboard is imaged to the multispectral camera, and then the multispectral camera transmits a standard black reflected image of the first pass band to the embedded main controller;
after the standard black-and-white reflection image of the first pass band is acquired, the embedded main controller starts the filter wheel controller to cut a second band pass filter into the laser optical axis, starts the switcher to cut the standard blackboard into the sample chamber, cuts the standard whiteboard into the substrate serving as the sample chamber, at the moment, the reflection light of the second pass band of the standard whiteboard is imaged to the multi-spectral camera, and then the multi-spectral camera transmits the standard white reflection image of the second pass band to the embedded main controller; then, the embedded main controller starts a switcher to cut the standard white board out of the sample chamber and cut the standard blackboard into the substrate serving as the sample chamber, reflected light of a second pass band of the standard blackboard is imaged to the multispectral camera at the moment, and then the multispectral camera transmits a standard black reflected image of the second pass band to the embedded main controller; thereby completing the acquisition of the standard black-and-white reflection image of the second passband;
similarly, completing the black-and-white reflection image acquisition of the third, fourth and.
2) Terahertz scanning imaging calibration
The embedded main controller controls to close the super-continuum spectrum laser and the multi-spectrum camera; the embedded main controller starts the switcher to cut the standard blackboard into a sample chamber and cut the standard whiteboard into a substrate serving as the sample chamber; the embedded main controller controls to start the terahertz source and the terahertz sensor, starts the terahertz scanning galvanometer switching and the rotary controller to switch the scanning galvanometer into the main optical axis, and the scanning galvanometer is in a working state at the moment; the terahertz scanning galvanometer switching and rotating controller controls the scanning galvanometer to carry out two-axis scanning, the reflection of the terahertz scanning galvanometer covers the whole scanning range in Hertz waves, and in the scanning process, a terahertz sensor collects terahertz reflection echo signals of a standard white board point by point and transmits the terahertz reflection echo signals to the embedded main controller, and the embedded main controller processes the signals to obtain a standard terahertz reflection white image;
then, the embedded main controller starts a switcher to cut the standard white board out of the sample chamber and cut the standard blackboard into a substrate serving as the sample chamber; the terahertz scanning galvanometer switching and rotating controller controls the scanning galvanometer to carry out two-axis scanning, and in the scanning process, a terahertz sensor collects standard blackboard terahertz reflection echo signals point by point and transmits the signals to the embedded main controller, and the embedded main controller processes the signals to obtain a standard terahertz reflection black image;
3) laser multispectral imaging
The embedded main controller controls to close the terahertz source and the terahertz sensor, starts the terahertz scanning galvanometer switching and rotates the controller to cut the scanning galvanometer out of a main optical axis, and the scanning galvanometer is in a non-working state at the moment;
after a feather or hide sample of a rare animal is put into a sample clip, the sample clip is inserted into a corresponding notch of a sample chamber; the embedded main controller controls to start the supercontinuum laser and the multispectral camera, the embedded main controller starts the filter wheel controller to cut the first plate, the second plate, the third plate and the last plate of the band-pass filter into the laser optical axis in sequence, and meanwhile, the multispectral camera acquires black bottom reflection images of the sample under uniform laser illumination of each pass band and with a standard blackboard as a background in sequence and transmits the black bottom reflection images to the embedded main controller;
4) terahertz scanning imaging
The embedded main controller controls to close the super-continuum spectrum laser and the multi-spectrum camera; the embedded main controller controls to start the terahertz source and the terahertz sensor, starts the terahertz scanning galvanometer switching and the rotary controller to switch the scanning galvanometer into the main optical axis, and the scanning galvanometer is in a working state at the moment; the terahertz scanning galvanometer switching and rotating controller controls the scanning galvanometer to carry out two-axis scanning, in the scanning process, a terahertz sensor collects terahertz reflection echo signals of a sample with a standard blackboard as a background point by point, the terahertz reflection echo signals are transmitted to the embedded main controller, and the embedded main controller processes the signals to obtain a standard terahertz black bottom reflection image of the sample;
5) image post-processing
The embedded main controller subtracts the corresponding passband standard black reflection image and standard terahertz reflection black image from the corresponding passband black bottom reflection image and terahertz black bottom reflection image of the sample respectively, and then divides the corresponding passband standard white reflection image and standard terahertz reflection white image respectively, so as to obtain each passband standard reflectivity image and terahertz standard reflectivity image of the sample, namely a supercontinuum standard reflectivity image; the embedded main controller converges the supercontinuum standard reflectivity image of the rare animal feather or leather sample and the species, origin and organization information thereof to construct a biological resource database of the rare animal feather or leather sample, and transmits the database information of the rare animal feather or leather sample to a cloud system of an entry and exit department through a wireless local area network transceiver network; after a large amount of rare animal feather or hide database is constructed based on the imager, the imager is used for detecting unknown samples to obtain supercontinuum standard reflectivity images, and then the images are searched, compared and identified with the rare animal feather or hide database, so that the origin tracing and species identification can be carried out.
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