CN107715093B

CN107715093B - Ginger juice scald ointment and preparation method thereof

Info

Publication number: CN107715093B
Application number: CN201711099015.8A
Authority: CN
Inventors: 唐晓珍; 侯婴惠; 位思清; 王帅; 乔旭光
Original assignee: Shandong Agricultural University
Current assignee: Shandong Agricultural University
Priority date: 2017-11-09
Filing date: 2017-11-09
Publication date: 2020-12-04
Anticipated expiration: 2037-11-09
Also published as: CN107715093A

Abstract

The invention discloses ginger juice scald ointment and a preparation method thereof, wherein the scald ointment is prepared from the following components: sodium dodecyl sulfate, glycerol, ginger juice, stearyl alcohol, liquid paraffin and vaseline; preparing juice from rhizoma Zingiberis recens, mixing oil phase three materials including stearyl alcohol, liquid paraffin and vaseline, heating in water bath to dissolve; heating water bath to melt water-phase sodium dodecyl sulfate, glycerol and rhizoma Zingiberis recens juice to the same temperature as oil phase; slowly pouring the oil phase into the water phase, slowly stirring the mixed ointment at room temperature, and cooling to obtain the ointment. The scald ointment prepared by the invention can realize the purpose of prolonging the storage period of the ointment without adding preservatives and other bacteriostatic agents. The prepared ointment can quickly relieve swelling and pain, prevent corrosion and inhibit bacteria, promote tissue regeneration and organize skin, and the product has low raw material cost, simple preparation process and convenient and safe use.

Description

Ginger juice scald ointment and preparation method thereof

Technical Field

The invention relates to an external ointment for treating burns and scalds, belonging to the field of burns and scalds.

Background

Burn and scald are injuries frequently encountered in daily life and production, wherein superficial second-degree scald is the main clinical practice. At present, the medicines for treating burns and scalds are various, and mainly comprise compound traditional Chinese medicines (such as Jingwanhong ointment), western medicines (such as neomycin sulfate ointment), external dressings (such as Aikangyin auxiliary materials), various folk secret recipes and the like. These methods, while effective in treating burns, have a number of drawbacks. For the components of the compound traditional Chinese medicine, the real components and action mechanisms of the medicine effect cannot be clearly developed by people in many times, so that most of the components are not accepted by modern medicine; western medicines for scald are generally various antibiotics, and the body is easy to generate drug resistance after long-term use; external treatment dressings such as medical pigskin, allogenic skin and the like have certain curative effect in clinical application, but the required treatment cost is too expensive, and most patients cannot bear the treatment cost; the medical hygiene of the folk secret prescription is not solved, and the folk secret prescription is easy to infect after being used, the wound surface is healed slowly, scars are easy to leave in the later treatment period, and the folk secret prescription has great influence on the mind and body of a patient.

Disclosure of Invention

Modern burn and scald recovery theory considers that the best result of treating burn and scald is to achieve high cure rate and good recovery of both function and appearance.

In order to overcome the defects of the prior art, the invention provides the ginger juice scald ointment capable of effectively treating superficial second-degree burns and scalds and the preparation method thereof. The technical scheme adopted by the invention is as follows:

a ginger juice scald ointment is prepared from the following components:

the composition of the water phase is as follows: 1-3 parts of sodium dodecyl sulfate, 4-7 parts of glycerol and 57-74 parts of ginger juice by volume;

the oil phase comprises the following components: 8-12 parts of stearyl alcohol, 5-9 parts of liquid paraffin and 8-12 parts of vaseline by weight;

the weight/volume ratio is g/mL.

Preferably, the composition is prepared from the following components:

the composition of the water phase is as follows: 1 part by weight of sodium dodecyl sulfate, 5 parts by volume of glycerol and 67 parts by volume of ginger juice;

the oil phase comprises the following components: 10 parts of stearyl alcohol, 7 parts of liquid paraffin and 10 parts of vaseline.

The preparation method comprises the following steps:

(1) preparing ginger juice: cutting and pulping the cleaned ginger, and filtering to obtain filtrate to obtain ginger juice;

(2) mixing the three substances of the oil phase, namely stearyl alcohol, liquid paraffin and vaseline according to the weight ratio, heating in a water bath, and dissolving at 60-100 ℃;

(3) filtering the melted oil phase, and removing residues;

(4) mixing three substances of sodium dodecyl sulfate and glycerol in the water phase and the ginger juice obtained in the step (1) according to the weight ratio, heating in a water bath and melting to the same temperature as the oil phase mixture;

(5) after the two phases are melted, slowly pouring the oil phase into the water phase, slowly stirring the mixed ointment at room temperature, and cooling to obtain the ointment.

The filtration in the step (1) is a multilayer gauze filtration.

The prepared ointment is light yellow grease.

The invention is effectively applicable to I-degree and superficial II-degree burns and scalds. The using method comprises the following steps: after conventional cleaning and disinfection, the ointment is applied to the wound twice a day without dressing.

Sheng Jiang warms stomach and stops vomiting, and is mainly used for treating stomach cold, vomiting and cough and asthma caused by wind-cold. The contained gingerol, curcumin, ginger essential oil, ginger protease and other substances can inhibit platelet aggregation, prevent blood coagulation and thrombosis; can accelerate the absorption of the exudate, and has the functions of detumescence, pain relief, antisepsis and bacteriostasis.

Has the advantages that: the ointment can realize the purpose of prolonging the storage period of the ointment without adding preservatives and other bacteriostatic agents. The prepared ointment can quickly relieve swelling and pain, prevent corrosion and inhibit bacteria, promote tissue regeneration and organize skin, and the product has low raw material cost, simple preparation process and convenient and safe use.

Drawings

FIG. 1 is a comparative graph of results of an orthogonal test after centrifugation of the creams;

FIG. 2 shows the recovery rate of functional components of ginger;

FIG. 3 is a comparison of the centrifuged temperature and light resistant creams;

FIG. 4 is a comparative diagram of the healing conditions of the wound surface of a mouse in different periods of the ginger juice scald ointment;

FIG. 5 shows the body weight changes of mice at different time periods;

FIG. 6 is a graph of the rate of wound healing over different periods of time;

FIG. 7 is a comparison of ginger juice scald ointment and skin slices of commercially available scald ointments;

FIG. 8 is a graph comparing the results of slicing the scalded parts of male and female mice.

The specific implementation mode is as follows:

the present invention will be further described with reference to examples and experimental examples.

Preparing raw materials:

ginger: shandong Jiang Hai food science and technology Co., Ltd;

glycerol: tianjin Kaiton Co., Ltd;

sodium dodecyl sulfate: tianjin Kaiton Co Ltd

Vaseline: tianjin, Yongda chemical reagent, Inc.;

liquid paraffin: tianjin Kaiton Co., Ltd;

stearyl alcohol: bodi chemical Co., Ltd, Tianjin. .

Example 1 preparation of ginger juice scald ointment a

(1) Preparing ginger juice: cutting and pulping the cleaned ginger, filtering the ginger by using four layers of gauze, and taking 67mL of ginger juice filtrate;

(2) putting 10g of stearyl alcohol, 7mL of liquid paraffin and 10g of vaseline of the three substances in the oil phase into the same beaker, heating in a water bath, and dissolving at 80 ℃;

(3) filtering the melted oil phase substances, and removing residues;

(4) putting 1g of sodium dodecyl sulfate, 5mL of glycerol and 67mL of ginger juice in the water phase into the same beaker, heating in a water bath, and melting until the temperature is the same as that of the oil phase mixture;

(5) after the two phases are melted, slowly pouring the oil phase into a water phase beaker, slowly stirring the mixed liquid at room temperature uniformly, and cooling to paste to obtain the ginger juice scald ointment A. The prepared ointment is light yellow grease.

Example 2 preparation of ginger juice scald ointment B

(1) Preparing ginger juice: cutting and pulping the cleaned ginger, filtering the ginger by using three layers of gauze, and taking 74mL of ginger juice filtrate;

(2) putting 8g of stearyl alcohol, 5mL of liquid paraffin and 8g of vaseline in the oil phase into the same beaker, heating in a water bath, and dissolving at 60 ℃;

(3) filtering the melted oil phase material to remove residues;

(4) putting 1g of sodium dodecyl sulfate, 4mL of glycerol and 74mL of ginger juice in the water phase into the same beaker, heating in a water bath, and melting until the temperature is the same as that of the oil phase mixture;

(5) after the two phases are melted, slowly pouring the oil phase into a water phase beaker, slowly stirring the mixed liquid at room temperature uniformly, and cooling to paste to obtain the ginger juice scald ointment B. The prepared ointment is light yellow grease.

Example 3 preparation of ginger juice scald ointment C

(1) Preparing ginger juice: cutting and pulping the cleaned ginger, filtering the ginger by using three layers of gauze, and taking 57mL of ginger juice filtrate;

(2) putting 12g of stearyl alcohol, 9mL of liquid paraffin and 12g of vaseline in an oil phase into the same beaker, heating in a water bath, and dissolving at 100 ℃;

(3) filtering the melted oil phase substances, and removing residues;

(4) putting 1g of sodium dodecyl sulfate, 7mL of glycerol and 57mL of ginger juice in the water phase into the same beaker, heating in a water bath, and melting until the temperature is the same as that of the oil phase mixture;

(5) after the two phases are melted, slowly pouring the oil phase into a water phase beaker, slowly stirring the mixed liquid at room temperature uniformly, and cooling to paste to obtain the ginger juice scald ointment C. The prepared ointment is light yellow grease.

Experimental example 1 Process formulation screening

(1) Screening of ginger juice cream matrix

The cream base is not only an excipient of the ointment, but also a carrier of the medicine, and has important influence on the quality of the cream and the release and absorption of the medicine. Compared with the W/O type matrix, the O/W type emulsion matrix has the advantages of small greasiness, easy cleaning, high transdermal absorption and drug release speed, and is more suitable for the treatment requirement of scalded skin, so the O/W type emulsion matrix is selected.

(2) Screening of the dosage range of the auxiliary materials

Preparing 50g of emulsifiable paste, determining that 5g of vaseline and 2.5g of glycerol are added into the ginger juice scald ointment according to the conventional dosage range of auxiliary materials in the cream required by national standards, and determining the ginger juice according to the total amount difference.

The formulations in table 1 were designed according to the dosage range of the national standard emulsifier adjuvants. Taking the oil phase and the water phase, respectively placing in No. 1 and No. 2 beakers, and heating in water bath to 80 ℃. Heating the two phases in water bath to 80 deg.C, maintaining the temperature for 5min, slowly adding the oil phase into the water phase in a trickle state, cooling at room temperature, and stirring to obtain cream. Table 2 shows that the ginger juice cream prepared according to formula 1 has the best appearance, uniform color, fine texture, easy application, no emulsion stratification after centrifugation, and higher stability than the latter 5 groups. However, the cream prepared by the formula has an elutriation phenomenon after centrifugation, and an unstable factor still exists, so that an optimal formula of the ginger cream needs to be screened by an orthogonal test on the basis.

TABLE 1 screening of base prescriptions

TABLE 2 screening score sheet for prescription of substrate

(3) Prescription screening

As shown in Table 3, L was used as 4 factors to be examined, i.e., stearyl alcohol (A), liquid paraffin (B), sodium lauryl sulfate (C) and emulsification temperature (D)⁹(3⁴) The orthogonal table preferably selects the optimal formula and the emulsification temperature by taking the emulsification effect as an index (full score of 100).

TABLE 3 orthogonal factor horizon

Design L for orthogonality factor level in Table 3⁹(3⁴) The results of the orthogonal tests are shown in Table 4

TABLE 4 results of orthogonal experiments

TABLE 5 analysis of variance of orthogonal test results

As can be seen from the analysis of the range results in Table 4, RA>RD>RC>RB, the influence of the four factors on the quality of the emulsifiable paste is as follows: stearyl alcohol (A)>Emulsification temperature (D)>Sodium dodecyl sulfate (C)>Liquid paraffin (B). As can be seen from Table 5, stearyl alcohol, sodium lauryl sulfate, and emulsification temperature among the four factors significantly affected. As can be seen from comparison of FIG. 1, the cream of group 8 was uniform in color, fine, and moderate in dilution, and was most easily applied than the other creams; group 1 has granular feeling when being coated, and obvious emulsion layering phenomenon appears after centrifugation, and the two phases of the cream oil and the water are not completely combined; 2. the smearing performance of the

groups

4, 3, 5 and 7 is good, but the contents of stearic acid and paraffin serving as thickening agents in the formula are low, and the ointment is thin, so that the overall property and stability of the ointment are influenced; comparing with fig. 2, the recovery and utilization rate of functional components (curcumin, gingerol and ginger protease) of ginger are more stable in group 8 than in group 9. Thus, the optimal formulation for optimizing ginger juice cream within the design of the test is group 8, group A₃B₂C₁D₃Namely 5.0g of stearyl alcohol, 3.5mL of liquid paraffin, 0.5g of sodium dodecyl sulfate, 100 ℃ of emulsification temperature, 5g of vaseline, 2.5mL of glycerol and 33.5mL of ginger juice.

Verification experiment

According to A₃B₂C₁D₃Namely 5.0g of stearyl alcohol, 3.5mL of liquid paraffin, 0.5g of sodium dodecyl sulfate, the emulsifying temperature of 100 ℃, 5g of vaseline, 2.5mL of glycerol and 33.5mL of ginger juice, the comprehensive ointment score is 92.7 minutes which is higher than any result in the table 4, so 5.0g of stearyl alcohol, 3.5mL of liquid paraffin, 0.5g of sodium dodecyl sulfate, 5g of vaseline, 2.5mL of glycerol and 33.5mL of ginger juice are added, and the optimal ginger juice scald cream formula is obtained when the emulsifying temperature is 100 ℃.

Experimental example 2 index test was performed on cream A, B, C prepared in example

(1) Centrifugal test

50g of each of the samples A, B, C prepared in the examples was packaged in a centrifuge tube with a plug and centrifuged at 3000r/min for 20min, and no delamination occurred. FIG. 3 is a graph showing a comparison of temperature and light-affected cream after centrifugation.

(2) pH value detection

10g of each of the A, B, C samples prepared in the example was diluted with 50mL of distilled water, stirred uniformly, and allowed to stand for 5min to obtain uniform emulsions, which were measured by a pH meter to have pH values of 6.26, 6.35, and 6.33, respectively.

(3) Temperature and light influence test

Respectively taking 50g of each sample A, B, C prepared in the example, respectively placing into a transparent and lightproof plastic box, keeping the sample in a constant temperature box at-18 ℃, 4 ℃, 25 ℃ and 55 ℃ for observation for 24h, and inspecting the characteristics of the cream and the indexes such as the change of the effective components of the ginger. As a result, the ointments of examples 1, 2 and 3 are suitable for being stored in a dark place at room temperature of about 25 ℃, and are free from rancidity, discoloration, delamination and the like.

(4) Open package stability test

50g of each sample A, B, C prepared in the example is respectively placed in an environment with the temperature of 25 +/-2 ℃ and the relative humidity of 60% soil 5, ointment bottles are opened once in eight morning and eight evening, and the

samples

1, 7, 14 and 21d are taken to examine the characters of the cream, the change of effective components of ginger and the like, and the phenomena of rancidity, discoloration and delamination do not occur.

(5) Accelerated test

The sample A, B, C prepared in the example was placed in a cream bottle using an opaque plastic box package under accelerated test conditions (temperature 25. + -. 2 ℃ C., relative humidity 60% soil 5%) for 3 months with a relatively stable pH, no delamination and no odor change after application.

(6) Observation of retained sample

After being stored for half a year in a sealed manner at room temperature, the ginger juice scald ointment A, B, C prepared in the embodiment has stable property and does not have the phenomena of demulsification and delamination.

Experimental example 3 pharmacodynamic experiment of ginger juice scald ointment

(1) Principle of experiment

The rhizoma Zingiberis recens contains gingerol, curcumin, ginger essential oil, ginger protease, etc., and has effects in inhibiting blood platelet aggregation, and preventing blood coagulation and thrombosis; can accelerate the absorption of the exudate, and has the functions of detumescence, pain relief, antisepsis and bacteriostasis. The scald medicine can accelerate wound healing after acting on the wound, and the recovery effect of the mouse can be observed through indexes such as the mouse wound healing rate, weight change, peripheral blood convention, the result of taking pathological sections of the skin of the scald part and the like.

(2) Experimental methods

Preparing a scald model: 80 Kunming mice (18-22g) are selected, and the male and female mice are half-half. The scald ointment groups are randomly divided into four groups, namely a scald ointment group (using the ginger juice scald ointment A prepared in example 1), a superficial II-degree scald negative control group (blank group), a superficial II-degree scald blank control group (ointment matrix without ginger juice), and a commercially available scald medicine group is smeared. Each group of male and female mice had 10 mice. The mouse is fixed on a table, and the model is scalded for 5s (the buttocks and the hair are sheared on the same day) by using the electric collateral iron with the temperature of 80 ℃ to form a superficial II-degree scald model.

Administering drugs to mice with superficial II-degree scald: after 24h of molding, the ointment is applied to the wound twice a day without binding after conventional cleaning and disinfection. The experimental group of mice was coated with ginger juice scald ointment, the blank control group was coated with ointment base, the negative control group was not coated with any drug, and the commercially available scald drug was coated with the commercially available Huatuo burn ointment. The mice were guaranteed adequate water and food daily.

Collecting data and samples: and collecting data of body weight, blood routine, wound healing rate and skin pathological section every 5 days after the scald.

Fig. 4 is a comparative diagram showing the healing condition of the wound surface of a mouse in different periods of the ginger juice scald ointment.

(3) Data result analysis

First, irritation test and allergy test

The buttocks of the mice were cut to remove hairs, and the scald ointment of ginger juice prepared in examples 1, 2 and 3 was applied to the clipped parts and the unhaired parts, respectively, and compared, no irritation or allergic reaction was observed.

② the change of the body weight of the mouse in different periods

After scald, the medicine is applied, because the medicine stimulates immune cells of the body, the mice are reduced in food intake and water intake due to pain, and the weight is obviously reduced in about five to ten days. In the later period, the wound is healed due to the exertion of the drug effect, and the body weight is gradually increased. As can be seen from fig. 5, the weight loss of the ginger juice scald ointment group and the commercially available scald medicine group is more obvious than that of the blank control group and the negative control group 5 days after scald. The later-period drug effect is obvious, and the weight gain of the mice in the ginger juice scald ointment group is extremely obvious.

(iii) rate of wound healing

After 3-5 days of scald, the whole layer of skin and subcutaneous tissue at the edge of wound moves to the center, so that the wound is rapidly reduced until 15 days or so. Wound contraction is caused by the traction of new myofibroblasts at the wound edge, and the wound healing rate is (area of wound at early stage of scald-area of wound at later stage of scald)/area at early stage of scald. It can be seen from fig. 6 that the healing rate of the ginger juice scald ointment group is obviously higher than that of the blank control group and the negative control group in the same period, and the healing rate is in a straight-line increase.

Results of routine examination of peripheral blood

TABLE 6 peripheral blood test results

Increased peripheral blood leukocyte counts are a common manifestation of inflammation. As can be seen from Table 6, inflammatory cells in mice in 5 days after the ointment group is scalded by the ginger juice are obviously higher than those in the negative control group and the blank control group, inflammatory cells can be rapidly activated by aggregation, and the immune cells can limit inflammatory factors and prevent pathogenic microorganisms from spreading all over the body. The routine index of blood of the ginger juice scald ointment group tends to be normal after 20 days.

Comparison of ginger juice scald ointment with skin slices of commercially available scald ointment

Fig. 7 is a comparison graph of ginger juice scald ointment and skin slices of commercially available scald ointment.

After the 5 th day after different groups of medicines are smeared, the immune system is activated, and leukocytes swim out of blood vessels and are gathered to the inflammation focus through chemotaxis. On the 10 th day after scald, subcutaneous hair follicles of the scald ointment group scalded by the ginger juice are clearly visible, but the fasciculate layers are not uniformly arranged, inflammatory cells of the scald ointment group sold in the market still gather at inflammatory parts, and the cuticle begins to repair. The appearance of the wound of the ginger juice group mice is well repaired on the 15 th day after the scald, the subcutaneous nerve begins to develop, and the hair follicle of the commercial scald medicament begins to develop at the same time. The comparison result shows that the ginger juice and the scald ointment can treat superficial II scalds, and the later-stage ginger juice group scald ointment has faster drug effect than the commercially available scald ointment.

Sixthly, the ginger juice scald ointment is used for contrasting male and female mice

FIG. 8 is a graph showing the comparison of the results of the sections of the scalded parts of male and female mice.

The male mouse is easy to get on the shelf due to its natural movement, and the wound is easy to be pulled and infected. After the ginger juice scald ointment is acted on a mouse, the skin cuticle of a male mouse is still seriously damaged on the 10 th day, and the skin hair follicle of a female mouse is well developed; 15 from the appearance, the wound skin of the female mice was well repaired, subcutaneous pacinian corpuscles began to develop, and this part of the cutaneous nerve began to be repaired. The hair follicles of the male mouse and the like are well developed, and the comparison of the effects in the same period obviously discovers that the treatment effect of the ginger juice scald ointment on the female mouse is superior to that of the male mouse.

The experimental results are analyzed, and the ginger juice scald ointment can rapidly activate immune cells in a mouse body in the early stage after acting on superficial II-degree scald skin, and has a better drug effect on female mice as compared with a commercially available scald drug in the later stage when promoting the healing speed of the mouse skin.

The foregoing is only a preferred embodiment of the present invention. Experimental modifications that can be made without departing from the principles of the present invention are within the scope of the invention.

Claims

1. The ginger juice scald ointment is characterized by being prepared from the following components:

the weight/volume ratio is g/mL;

the preparation method comprises the following steps:

(2) mixing three substances of an oil phase, namely stearyl alcohol, liquid paraffin and vaseline according to a ratio, heating in a water bath, and dissolving at 60-100 ℃;

(3) filtering the melted oil phase, and removing residues;

(4) mixing three substances of sodium dodecyl sulfate and glycerol in the water phase and the ginger juice obtained in the step (1) according to a ratio, heating in a water bath and melting to the same temperature as the oil phase mixture;

2. The ginger juice scald ointment according to claim 1, which is prepared from the following components:

3. The preparation method of the ginger juice scald ointment as set forth in claim 1 or 2, characterized in that the preparation method comprises the following steps:

(3) filtering the melted oil phase, and removing residues;

4. The method for preparing ginger juice scald ointment as claimed in claim 3, wherein said filtering in step (1) is performed by using a multi-layer gauze.

5. The method for preparing ginger juice scald ointment according to claim 3 or 4, wherein the dissolution temperature in the step (2) is 80 ℃.