CN107709574B - 生物组织样品的分子概况的空间作图 - Google Patents

生物组织样品的分子概况的空间作图 Download PDF

Info

Publication number
CN107709574B
CN107709574B CN201680034754.6A CN201680034754A CN107709574B CN 107709574 B CN107709574 B CN 107709574B CN 201680034754 A CN201680034754 A CN 201680034754A CN 107709574 B CN107709574 B CN 107709574B
Authority
CN
China
Prior art keywords
nucleic acid
roi
sample
oligonucleotide probe
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201680034754.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN107709574A (zh
Inventor
M.范德里伊
R.维姆伯格-弗里伊德
A.皮尔里克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Koninklijke Philips NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips NV filed Critical Koninklijke Philips NV
Publication of CN107709574A publication Critical patent/CN107709574A/zh
Application granted granted Critical
Publication of CN107709574B publication Critical patent/CN107709574B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C12Q2543/101—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis in situ amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cell Biology (AREA)
CN201680034754.6A 2015-04-14 2016-04-13 生物组织样品的分子概况的空间作图 Active CN107709574B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15163481 2015-04-14
EP15163481.3 2015-04-14
PCT/EP2016/058067 WO2016166128A1 (en) 2015-04-14 2016-04-13 Spatial mapping of molecular profiles of biological tissue samples

Publications (2)

Publication Number Publication Date
CN107709574A CN107709574A (zh) 2018-02-16
CN107709574B true CN107709574B (zh) 2021-10-01

Family

ID=52991497

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680034754.6A Active CN107709574B (zh) 2015-04-14 2016-04-13 生物组织样品的分子概况的空间作图

Country Status (7)

Country Link
US (1) US10724078B2 (https=)
EP (1) EP3283641B1 (https=)
JP (1) JP6875998B2 (https=)
CN (1) CN107709574B (https=)
BR (1) BR112017021993A2 (https=)
RU (1) RU2733545C2 (https=)
WO (1) WO2016166128A1 (https=)

Families Citing this family (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US20180057873A1 (en) * 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
US10724089B2 (en) * 2016-10-06 2020-07-28 The Board Of Trustees Of The University Of Illinois Spatial molecular analysis of tissue
CN111051526A (zh) * 2017-05-23 2020-04-21 生捷科技控股公司 用于实施生物分子空间分布分析的方法
WO2019169407A1 (en) 2018-03-02 2019-09-06 Quantumcyte, Inc. Methods, compositions, and devices for isolation and expression analysis of regions of interest from a tissue
EP3844308A1 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Resolving spatial arrays
EP3844304B1 (en) 2018-08-28 2024-10-02 10X Genomics, Inc. Methods for generating spatially barcoded arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
CN113543737B (zh) * 2018-12-31 2024-12-27 阿瓦瓦公司 用于组织治疗的系统和方法
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN114174531A (zh) * 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
WO2020190509A1 (en) 2019-03-15 2020-09-24 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4038546B1 (en) 2019-10-01 2024-08-21 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
CA3153886A1 (en) * 2019-10-18 2021-04-22 Nanostring Technologies, Inc. Systems and methods for spatial mapping of expression profiling
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021097255A1 (en) 2019-11-13 2021-05-20 10X Genomics, Inc. Generating capture probes for spatial analysis
AU2020388047A1 (en) 2019-11-18 2022-06-23 10X Genomics, Inc. Systems and methods for tissue classification
WO2021102039A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
EP4435718A3 (en) 2019-11-22 2024-12-18 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021133849A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115715329A (zh) 2020-01-10 2023-02-24 10X基因组学有限公司 确定生物样品中靶核酸位置的方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US20230081381A1 (en) 2020-02-20 2023-03-16 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
EP4484571A3 (en) 2020-02-21 2025-03-19 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
WO2021225900A1 (en) 2020-05-04 2021-11-11 10X Genomics, Inc. Spatial transcriptomic transfer modes
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237056A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Rna integrity analysis in a biological sample
EP4553168A3 (en) 2020-05-22 2025-08-27 10x Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158054B1 (en) 2020-06-02 2025-04-16 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US20230279474A1 (en) 2020-06-10 2023-09-07 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
US20230235384A1 (en) * 2020-06-18 2023-07-27 Nanostring Technologies, Inc. Compositions and methods for in situ single cell analysis using enzymatic nucleic acid extension
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US20230287475A1 (en) 2020-07-31 2023-09-14 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US12580044B1 (en) 2020-09-02 2026-03-17 10X Genomics, Inc. Systems and methods for identifying cells that are antigen-specific for an immunogenic feature
EP4530358A3 (en) 2020-09-15 2025-06-18 10x Genomics, Inc. Methods of releasing an extended capture probe from a substrate and uses of the same
AU2021345133A1 (en) 2020-09-16 2023-03-30 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
EP4491742B1 (en) 2020-09-18 2026-03-04 10X Genomics, Inc. Sample handling apparatus and image registration methods
US20240033743A1 (en) 2020-09-18 2024-02-01 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
AU2021366701A1 (en) 2020-10-22 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification
AU2021376399A1 (en) 2020-11-06 2023-06-15 10X Genomics, Inc. Compositions and methods for binding an analyte to a capture probe
AU2021385065A1 (en) 2020-11-18 2023-06-15 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022147005A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Methods for analyte capture determination
WO2022147296A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
WO2022197490A1 (en) * 2021-03-16 2022-09-22 Scale Biosciences Inc. Method for adding spatially-addressable barcodes to nucleic acids of a cellular sample in situ
ES3008686T3 (en) 2021-03-18 2025-03-24 10X Genomics Inc Multiplex capture of gene and protein expression from a biological sample
EP4620481A3 (en) 2021-04-05 2025-12-03 10X Genomics, Inc. Recombinant ligase composition and uses thereof
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022226057A1 (en) 2021-04-20 2022-10-27 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20240368711A1 (en) 2021-06-22 2024-11-07 10X Genomics, Inc. Spatial detection of sars-cov-2 using templated ligation
EP4352252B1 (en) 2021-07-13 2026-01-28 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023018799A1 (en) 2021-08-12 2023-02-16 10X Genomics, Inc. Methods, compositions and systems for identifying antigen-binding molecules
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
US20240378734A1 (en) 2021-09-17 2024-11-14 10X Genomics, Inc. Systems and methods for image registration or alignment
WO2023076345A1 (en) 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023086824A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods for identification of antigen-binding molecules
WO2023102313A1 (en) 2021-11-30 2023-06-08 10X Genomics, Inc. Systems and methods for identifying regions of aneuploidy in a tissue
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023150163A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from lymphatic tissue
WO2023150098A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, kits, compositions, and systems for spatial analysis
WO2023150171A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from glioblastoma samples
JP2025504714A (ja) * 2022-02-14 2025-02-17 ミルテニー バイオテック ベー．フェー． ウント コー． カー・ゲー ミクロトーム処理した組織スライス上でのオリゴヌクレオチドの直接合成
EP4479933A1 (en) 2022-02-15 2024-12-25 10x Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
WO2023172670A2 (en) 2022-03-11 2023-09-14 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US20250251403A1 (en) 2022-04-12 2025-08-07 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
WO2023215552A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Molecular barcode readers for analyte detection
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
WO2023229982A2 (en) 2022-05-24 2023-11-30 10X Genomics, Inc. Porous structure confinement for convection suppression
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4554973A1 (en) 2022-07-13 2025-05-21 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015578A1 (en) 2022-07-15 2024-01-18 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
EP4565870A1 (en) 2022-08-05 2025-06-11 10x Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (en) 2022-08-10 2024-02-15 10X Genomics, Inc. Systems and methods for colocalization
WO2024035844A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Methods for reducing capture of analytes
EP4577670A1 (en) 2022-08-24 2025-07-02 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
EP4573208A1 (en) 2022-10-10 2025-06-25 10X Genomics, Inc. In vitro transcription of spatially captured nucleic acids
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
WO2024086167A2 (en) 2022-10-17 2024-04-25 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
JPWO2024116670A1 (https=) * 2022-11-30 2024-06-06
EP4638788A1 (en) 2022-12-21 2025-10-29 10X Genomics, Inc. Analysis of analytes and spatial gene expression
WO2024145441A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for determining a location of a target nucleic acid in a fixed biological sample
WO2024145224A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Compositions, methods, and systems for high resolution spatial analysis
EP4486913B1 (en) 2022-12-30 2025-07-16 10x Genomics, Inc. Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding
WO2024145445A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods of capturing target analytes
EP4689174A1 (en) 2023-03-28 2026-02-11 10X Genomics, Inc. Methods, compositions, and kits for reducing analyte mislocalization
US20250257393A1 (en) 2023-04-21 2025-08-14 10X Genomics, Inc. Methods and compositions for detecting nucleic acids in a fixed biological sample
WO2024238625A1 (en) 2023-05-15 2024-11-21 10X Genomics, Inc. Spatial antibody data normalization
WO2024238900A1 (en) 2023-05-17 2024-11-21 10X Genomics, Inc. Methods of reducing mislocalization of an analyte
WO2024238992A1 (en) 2023-05-18 2024-11-21 10X Genomics, Inc. Engineered non-strand displacing family b polymerases for reverse transcription and gap-fill applications
WO2024254316A1 (en) 2023-06-07 2024-12-12 10X Genomics, Inc. Compositions and methods for identifying blood clots in biological samples
EP4728062A1 (en) 2023-06-13 2026-04-22 10X Genomics, Inc. Sequence specific dna binding proteins
WO2025029605A1 (en) 2023-07-28 2025-02-06 10X Genomics, Inc. Methods and compositions for detecting genetic variants using capture probes and primer extension
WO2025029627A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods, compositions, and kits for spatial detection of genetic variants
EP4581160A1 (en) 2023-08-24 2025-07-09 10X Genomics, Inc. Methods, kits, and compositions for spatial detection of genetic variants
WO2025072119A1 (en) 2023-09-26 2025-04-03 10X Genomics, Inc. Methods, compositions, and kits for detecting spatial chromosomal interactions
WO2025090912A1 (en) 2023-10-26 2025-05-01 10X Genomics, Inc. Spatial arrays containing reusable master probes
WO2025096581A1 (en) 2023-10-31 2025-05-08 10X Genomics, Inc. Methods of spatial analysis and single nuclei sequencing
WO2025101864A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Methods, compositions, and kits for reducing mislocalization of analytes in spatial analysis assays
WO2025101377A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Matrix-assisted spatial analysis of biological samples
US12442729B2 (en) 2023-11-13 2025-10-14 Singular Genomics Systems, Inc. Devices useful for sample manipulation
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2026024934A1 (en) 2024-07-25 2026-01-29 10X Genomics, Inc. Methods, compositions, and kits for spatial analysis by tagmentation
WO2026043885A1 (en) 2024-08-20 2026-02-26 10X Genomics, Inc. Methods and systems for preparing a nucleic acid library

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012140224A1 (en) * 2011-04-13 2012-10-18 Jonas Frisen Method and product for localised or spatial detection of nucleic acid in a tissue sample

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050123181A1 (en) 2003-10-08 2005-06-09 Philip Freund Automated microscope slide tissue sample mapping and image acquisition
JP5739871B2 (ja) * 2009-04-28 2015-06-24 コーニンクレッカ フィリップス エヌ ヴェ 生物学、組織学及び病理学のために使用する方法、装置、並びにデータキャリア
HRP20151294T1 (hr) * 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
CN104919299A (zh) 2012-10-03 2015-09-16 皇家飞利浦有限公司 组合的样品检查
EP3013983B1 (en) * 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012140224A1 (en) * 2011-04-13 2012-10-18 Jonas Frisen Method and product for localised or spatial detection of nucleic acid in a tissue sample

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
In situ sequencing for RNA analysis in preserved tissue and cells;RONGQIN HE等;《NATURE METHODS》;20130714;第10卷(第9期);第857-858,861-862页 *

Also Published As

Publication number Publication date
EP3283641A1 (en) 2018-02-21
WO2016166128A1 (en) 2016-10-20
JP6875998B2 (ja) 2021-05-26
US20180112261A1 (en) 2018-04-26
CN107709574A (zh) 2018-02-16
BR112017021993A2 (pt) 2018-07-31
EP3283641B1 (en) 2019-11-27
RU2017135434A (ru) 2019-04-08
US10724078B2 (en) 2020-07-28
JP2018516541A (ja) 2018-06-28
RU2733545C2 (ru) 2020-10-05
RU2017135434A3 (https=) 2019-10-02

Similar Documents

Publication Publication Date Title
CN107709574B (zh) 生物组织样品的分子概况的空间作图
US12076701B2 (en) Capturing oligonucleotides in spatial transcriptomics
CN116848263A (zh) 用于分析物检测的方法和组合物
EP4151748B1 (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN107810277B (zh) 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
CN108796058B (zh) 用于组织样本中核酸的局部或空间检测的方法和产品
JP2022549505A (ja) 空間的オミックス配列解析のための決定論的バーコーディング
CN113604547A (zh) 一种用于组织样本的高分辨率空间组学检测方法
US20250002983A1 (en) Massively parallel characterizations of multiple target types in multi-dimensional space
Dubey et al. Microarray technology: basic concept, protocols, and applications
HK40081866B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40129148A (zh) 生物样本的空间区别、多重核酸分析
HK40102430B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40102430A (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40062809B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40062808B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40080403B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
Ginsberg Microarray use for the analysis of the CNS
HK40062809A (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40062808A (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant