CN107703297B - Weak magnetic signal detection device - Google Patents
Weak magnetic signal detection device Download PDFInfo
- Publication number
- CN107703297B CN107703297B CN201710737948.9A CN201710737948A CN107703297B CN 107703297 B CN107703297 B CN 107703297B CN 201710737948 A CN201710737948 A CN 201710737948A CN 107703297 B CN107703297 B CN 107703297B
- Authority
- CN
- China
- Prior art keywords
- test strip
- magnetic signal
- probe
- weak magnetic
- detection device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/091—Constructional adaptation of the sensor to specific applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/093—Magnetoresistive devices using multilayer structures, e.g. giant magnetoresistance sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/096—Magnetoresistive devices anisotropic magnetoresistance sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/098—Magnetoresistive devices comprising tunnel junctions, e.g. tunnel magnetoresistance sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
Abstract
The invention relates to a weak magnetic signal detection device. A weak magnetic signal detection device can be used for detecting weak magnetic signals on a test strip, and comprises a bearing part and a floating probe, wherein a clamping groove is formed in the bearing part and used for fixing the test strip; wherein the floating probe is in elastic contact with the test strip fixed in the card slot and detects a magnetic signal on the test strip by relative parallel movement with the test strip. Through setting up floating probe for when utilizing test probe to carry out weak magnetism to the test paper strip and detecting, test probe elastic contact carry out relative motion with the test paper strip, and then promote precision and sensitivity to weak magnetism signal detection on the test paper strip.
Description
Technical Field
The invention relates to magnetic signal detection, in particular to a device for detecting weak magnetic signals on a test strip.
Background
At present, when a magnetoresistive signal detection instrument is used for detecting weak magnetism on a test strip (especially a lateral immunochromatography test strip), due to the limitations of the performance and technical conditions of various components, the parameters of the detection instrument, such as sensitivity, detection precision and the like, can not meet the current requirements of weak magnetism detection.
Disclosure of Invention
therefore, it is necessary to provide a weak magnetic signal detection device for detecting a weak magnetic signal on a test strip to improve the accuracy and sensitivity of detection of the magnetic signal on the test strip, so as to meet the high requirements of in vitro diagnosis of a current biological sample.
The invention provides a weak magnetic signal detection device, which is used for detecting a weak magnetic signal on a test strip, and comprises a bearing part and a floating probe, wherein the bearing part is provided with a clamping groove, and the clamping groove is used for fixing the test strip;
Wherein the floating probe is in resilient contact with the test strip held in the card slot and detects a magnetic signal on the test strip by relative translation with the test strip.
According to the weak magnetic detection device, the floating type test probe is in elastic contact with the test strip in the relative translation process, so that the detection precision and sensitivity of weak magnetic signals on the test strip are improved.
in one embodiment, the weak magnetic signal detection device is used for detecting a weak magnetic signal on a test strip carrying magnetic nanoparticles, and the weak magnetic signal detection device further comprises a magnet for generating a magnetic field for magnetizing the magnetic nanoparticles;
Wherein the floating probe is configured to detect a magnetic signal generated by the magnetic nanoparticles on the test strip after being magnetized.
in one embodiment thereof, the magnetic nanoparticles are superparamagnetic nanoparticles.
In one embodiment, the floating probe further comprises at least one magneto-resistive sensor element in resilient contact with the test strip held in the card slot and detecting a magnetic signal on the test strip by relative translation with the test strip.
in one embodiment thereof, the at least one magneto-resistive sensor element is one or more of the following: tunnel magnetoresistive magnetic sensor elements, giant magnetoresistive magnetic sensor elements, anisotropic magnetoresistive sensor elements.
In one embodiment thereof, the floating probe may further comprise a resilient component on which the at least one magneto-resistive sensor element is fixedly disposed;
Wherein the elastic member is in a compressed state when the magnetoresistive sensor element is in elastic contact with the test strip fixed in the card slot.
in one embodiment, the weak magnetic signal detection device may further include at least two baffles, the floating probe further includes a probe fixing plate, and the elastic component is fixedly connected to the at least one magnetoresistive sensor element through the probe fixing plate;
The at least two baffles are respectively and fixedly arranged on two sides of the probe fixing plate to limit the translation track of the probe fixing plate.
In one embodiment, the magnetic signal detection device may further include:
The power device is connected with the bearing part through a transmission device so as to drive the test strip fixed in the clamping groove to move relative to the floating probe.
In one embodiment, the power device is a stepper motor, and the transmission device is a screw or a pulley;
The step motor drives the bearing part to move through the screw rod or the belt wheel so as to drive the test strip fixed in the clamping groove to move relative to the floating probe.
In one embodiment, the card slot may include:
A base plate;
The upper cover is clamped on the bottom plate to form a cavity for fixedly placing the test strip;
Wherein, the upper cover is provided with a window for exposing the test strip fixedly placed in the cavity, so that the floating probe can pass through the window to be in elastic contact with the test strip.
In one embodiment, the strip is a lateral immunochromatographic strip.
Drawings
fig. 1 is a schematic structural view of a weak magnetic signal detection apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic view of the weak magnetic signal detection device shown in FIG. 1 in operation when detecting a weak magnetic signal of a test strip;
Fig. 3 is a schematic structural view of a weak magnetic signal detecting apparatus according to another embodiment of the present invention;
FIG. 4 is a schematic structural diagram of the card slot shown in FIG. 3, which is fixedly placed and carries the test strip;
FIG. 5 is a schematic structural diagram of the test strip in one embodiment;
FIG. 6 is a schematic diagram of the test strip shown in FIG. 5 after immunoreaction.
Detailed Description
In order to make the objects, technical means and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the scope of the invention.
fig. 1 is a schematic structural view of a weak magnetic signal detection apparatus according to an embodiment of the present invention; fig. 2 is a schematic diagram of an operating state of the weak magnetic signal detection device shown in fig. 1 when the weak magnetic signal detection device detects a weak magnetic signal of a test strip. As shown in fig. 1-2, a weak magnetic signal detection device can be used for detecting a weak magnetic signal on a test strip 3, and the weak magnetic signal detection device can include a bearing part 1, a floating probe 2 and a card slot 11; the bearing part 1 is provided with a slot 11 for fixing the test strip 3, and the floating probe 2 can elastically contact with the test strip 11 fixed in the slot 11, so that the floating probe 2 can detect the weak magnetic signal on the test strip 3 through the relative translation with the test strip 3. Referring to fig. 1, a slot 11 for fixing a test strip 3 can be fixedly carried on a carrier 1, and a floating probe 2 can be adjacent to the carrier 1 and disposed above the slot 11, so that when the floating probe 2 is used to detect a weak magnetic signal of the test strip 3, the floating probe 2 can keep elastic contact with the test strip 3 fixed in the slot 11 during a relative translation process; the test strip 3 may be a chromatographic test strip such as lateral immunochromatographic test strip.
For example, as shown in fig. 1 to 2, after a test strip 3 to be tested is fixed in the slot 11, the position of the floating probe 2 is adjusted up and down along the direction indicated by the arrow, so that the floating probe 2 contacts with the test strip 3, and since the floating probe 2 can move in the up and down direction indicated by the arrow in the figure, the floating probe 2 can be kept to have a preset elastic pressure on the test strip 3, so that the floating probe 2 can be kept in contact with the test strip 3 when the floating probe 2 moves left and right along the direction indicated by the arrow in fig. 2 for detection in the following process of making the floating probe 2 move left and right relative to the test strip 3 (or the carrying member 1) along the direction.
In another embodiment, because the process difficulty of maintaining the contact between the floating probe 2 and the test strip 3 all the time during the relative translation is high, the manufacturing cost of the corresponding device is increased, and during the detection process, the gap between the floating probe 2 and the test strip 3 is controlled within a preset range during the relative translation, for example, the gap is controlled within a range of a millimeter level and a distance below the millimeter level, i.e., the floating probe 2 and the test strip 3 maintain a small distance (e.g., less than 1mm) and can effectively maintain the stability of the distance, so that the requirements of the weak magnetic detection on accuracy, sensitivity and the like can be effectively met.
The weak magnetic detection device can be applied to detection of biomarkers, environmental toxins, micromolecular pollutants and the like, floating detection probes are arranged, floating movement of the detection probes can be kept when the test strip is detected, and then the magnetic field on the test strip can be detected in a short distance, so that magnetic field signals of magnetic particles with low concentration can be collected, and the sensitivity, the precision and the like of the detection of the weak magnetic field on the test strip can be effectively improved.
in one embodiment, as shown in fig. 1-2, the test strip 3 may carry magnetic nanoparticles (not shown), and the weak magnetic detection device may include a magnet (not shown) for generating an applied magnetic field, the magnet may be disposed on the carrying member 1 and adjacent to the fixed position of the test strip 3, and may also be disposed adjacent to the floating test probe 2, so as to enable the magnetic field generated by the magnet to effectively magnetize the magnetic nanoparticles before the weak magnetic detection is performed on the test strip 3, that is, the floating test probe 2 is configured to detect the magnetic field generated after the magnetic nanoparticles on the test strip 3 are magnetized. The magnet may be a soft or permanent magnet, i.e. as long as the magnetic field generated by the magnet, such as an alternating or constant magnetic field, is capable of effectively magnetizing the magnetic nanoparticles. In this embodiment, the applied magnetic field can effectively magnetize the magnetic nanoparticles, so that the accuracy and sensitivity of subsequent weak magnetic detection on the magnetic nanoparticles can be effectively improved, and in order to further improve the performance such as the accuracy and sensitivity of detection, the test strip 3 can be always in the magnetic field generated by the magnet in the process of weak magnetic detection on the test strip 3.
In one embodiment, the magnetic nanoparticles may be paramagnetic nanoparticles, ferromagnetic nanoparticles, or superparamagnetic nanoparticles. The superparamagnetic nanoparticle is a nanoparticle having magnetic responsiveness, the diameter of a single crystal particle of the superparamagnetic nanoparticle is generally below 30nm, and when the particle diameter of the magnetic nanoparticle is smaller than the superparamagnetic critical dimension of the magnetic nanoparticle, the particle enters a superparamagnetic state. The superparamagnetic nano-particles can be in a suspension state in liquid and can be magnetized to generate directional movement under the action of an external gradient magnetic field, so that the superparamagnetic nano-particles can be separated from a medium at a designated position; when the external magnetic field is removed, the magnetic separation device can be in a suspended state again, so that the magnetic separation device has the advantages of low magnetic separation cost, good dispersibility, good operability and the like.
In order to effectively magnetize the superparamagnetic nanoparticles with an external magnetic field when the magnetic nanoparticles are superparamagnetic nanoparticles, in one embodiment, the distance between the magnet and the test strip may be kept less than 0.5mm during magnetization; for example, the distance between the holding magnet and the test strip is 0.2mm, 0.4mm, 0.5mm, or the like, before and/or during the weak magnetic detection.
In another embodiment, the test strip may be a lateral immunochromatographic test strip carrying superparamagnetic nanoparticles, and after the lateral immunochromatographic test strip performs an antigen-antibody reaction, the superparamagnetic nanoparticles are used to form an anchoring magnetic aggregate or a magnetic aggregate fixed at a specific position, and then the fixed magnetic aggregate may be magnetized by an external magnetic field, and qualitative and quantitative analysis of a detection object may be completed by detecting a magnetic signal of the magnetic field generated by the magnetized magnetic aggregate.
In one embodiment, as shown in fig. 1-2, the floating probe may include at least one magnetic resistance sensor element (not shown), and the detection of the weak magnetic signal of the test strip 3 is realized by making the at least one magnetic resistance sensor element perform a relative motion with respect to the test strip 3. The at least one Magneto-resistive sensing element may comprise one or more of a Tunneling Magneto-resistive (TMR) magnetic sensing element, a Giant Magneto-resistive (GMR) magnetic sensing element, or an Anisotropic Magneto-resistive (AMR) magnetic sensing element; the types and the number of the magneto-resistive sensor elements in the floating probe can be set according to actual requirements; for example, the floating probe can include four magnetic sensor elements, specifically, a TMR magnetic sensor element, an AMR magnetic sensor element, and two GMR magnetic sensor elements, to improve the sensitivity and accuracy of the weak magnetic signal detection device by combining the characteristics of different magnetic sensor elements.
in one embodiment, the magnetoresistive sensing elements of the at least one magnetoresistive sensing element are TMR magnetic sensor elements, that is, the TMR magnetic sensor elements (not shown) can elastically contact with the test strip 3 fixed in the card slot 11 and perform relative movement to detect a magnetic signal on the test strip 3. Because the TMR magnetoresistive sensing element has higher detection accuracy and sensitivity compared with, for example, a Hall (Hall) magnetoresistive sensing element, an AMR magnetoresistive sensing element, a GMR magnetoresistive sensing element, and the like, and also has advantages such as smaller power consumption, the TMR magnetoresistive sensing element can further improve weak magnetic detection performance such as accuracy and sensitivity of a detection device.
In one embodiment, as shown in fig. 1-2, the floating probe 2 may include an elastic component (not shown), the at least one magnetic resistance sensing element may be fixedly disposed on the elastic component, and when the test strip 3 is subjected to weak magnetic detection, the magnetic resistance sensing element may be pressed on the surface of the test strip 3 by pressing the elastic component, and meanwhile, the elastic component may be kept in a compressed state all the time during the weak magnetic detection, so as to ensure that the TMR magnetic sensor and the test strip 3 keep elastic contact during relative movement, thereby improving the performance of the weak magnetic detection, such as precision and sensitivity. When a plurality of magnetoresistive sensing elements are arranged, part or one of the magnetoresistive sensing elements can be kept in elastic contact with the test strip 3 when weak magnetic detection is carried out on part or one of the magnetoresistive sensing elements according to requirements and performance parameters of the magnetoresistive sensing elements, and the rest of the magnetoresistive sensing elements can be kept in a set interval range with the test strip.
In one embodiment, as shown in fig. 1-2, during the weak magnetic detection, the floating probe 2 may be configured to move only in the up-and-down direction, and some power devices are configured to drive the carrying member 1 to move in the left-and-right direction, that is, after the floating probe 2 moves to a preset position, the floating probe 2 is extruded onto the test strip 3 in a floating manner, and the floating probe 2 is fixed in the translation directions such as left-and-right, front-and-back, and the like, so that the floating probe 2 can only move in the floating manner up-and-down direction, such as through elastic components; then, the bearing part 1 is driven to translate by a power device such as a stepping motor through a transmission device such as a screw rod and a belt wheel, so that the floating probe 2 and the test strip 3 move relatively in the horizontal direction, and the weak magnetic detection of the test strip 3 is realized.
In this embodiment, the damages to the circuit and the equipment caused by the frequent movement of the floating probe 2 due to the related connections such as the magnetic sensor and the magnetic signal acquisition, conversion and processing on the floating probe 2 and/or the equipment can be effectively avoided, and meanwhile, the bearing part 1 is driven by the power equipment such as the stepping motor to translate through the transmission device, so that the speed, the stability and the like of the relative movement between the floating probe 2 and the test strip 3 can be effectively ensured, so as to generate a relatively strong and stable current signal, and simultaneously, the interference of the geomagnetic field and the overseas magnetic field to the measurement signal can be effectively avoided, and further, the performance of the weak magnetic detection equipment and the stability of the weak magnetic detection can be further improved.
In addition, because step motor has that the step value does not receive the influence of various interference factors, the error can not long-term accumulation and control performance is good, starts, parks, upset all accomplish advantages such as in a few pulses, so make in this embodiment utilize step motor to drive the motion of test paper strip 3 more steady and accurate to can make can effectual promotion weak magnetism check out test set's performance and weak magnetism stability of detecting.
Fig. 3 is a schematic structural diagram of a weak magnetic signal detection apparatus according to another embodiment of the present invention. As shown in fig. 3, a specific weak magnetic signal detecting apparatus is exemplified below, and the weak magnetic signal detecting apparatus may include a bracket 10, where the bracket 10 is used for fixing various components in the weak magnetic signal detecting apparatus and provides a stable support and platform for weak magnetic signal detection. The probe fixing plate 102 is disposed on the above-mentioned bracket 10 through the upper and lower elastic assemblies 103, and the other side of the probe fixing plate 102 opposite to the elastic assemblies 103 is fixed with the probe part 101 with the TMR magnetic sensor element built therein, and the left baffle 104 and the right baffle 105 are respectively located on the two opposite sides of the probe fixing plate 102 and are both fixed on the bracket 10, so as to limit the translation track of the probe part 101 through the probe fixing plate 102, and make it move in a space ensuring the movement precision. The slider 203 for bearing the test strip is arranged below the probe part 101 in a translation manner, and the clamping groove 30 for containing the test strip is clamped in a clamping groove (not shown in the figure) formed in the slider 203, so that the TMR magnetic sensor can detect the weak magnetic signal of the test strip contained in the clamping groove 30 through the window 301. Stepping motor 201 is then connected with slider 203 through screw rod 202 to drive slider 203 through this screw rod 202 and carry out the translation, and then make the TMR magnetic sensor who sets up in probe part 101 hold the test paper strip in draw-in groove 30 relatively and carry out quick uniform motion, thereby realize the low magnetism of the high accuracy and the high sensitivity to the test paper strip and detect.
Fig. 4 is a schematic structural diagram of the card slot shown in fig. 3, which is fixedly placed and carries the test strip. As shown in fig. 4, the card slot 30 may include a bottom plate 307 and an upper cover 302 engaged with the bottom plate 307, the upper cover 302 is opened with a sample adding hole 303 for dropping a test sample, a positioning notch 304 for performing test positioning, a guide angle 305 for performing test guiding, and the like, a guide arc 306 for guiding is further provided at the exposed top end of the bottom plate 307, and a window 301 opened on the upper surface of the upper cover 302 is used for exposing a detection portion of the test strip 40 fixedly placed in the card slot 30, that is, the probe 101 is inserted into the window 301 to realize weak magnetic signal detection on the test strip 40.
As shown in fig. 3 to 4, in one embodiment, the stepping motor 201 can drive the screw 202 to rotate, so as to drive the sliding block 203 to move in a translational manner, such as back and forth; the sliding block 203 may be provided with a female thread corresponding to the screw 202, and a sample slot (not shown) for inserting into the card slot 30 may be opened at the top of the sliding block 203. The probe part 101, the probe fixing plate 102, the upper and lower elastic components 103, etc. constitute a part capable of floating up and down. Before the test strip 40 is detected, the TMR magnetic sensor in the probe part 101 is firstly attached to the surface of the test strip 40, once the test strip 40 moves forwards or backwards relative to the TMR magnetic sensor, the protruding part of the probe of the TMR magnetic sensor samples in the groove of the test strip 40, because the magnetized magnetic aggregate formed by the superparamagnetic particles is arranged in the groove of the test strip 40, the probe of the TMR magnetic sensor generates a corresponding electric signal by cutting the magnetic force line of the magnetic field generated by the magnetized magnetic aggregate, and then the weak magnetic detection of the test strip 40 is realized.
In another embodiment, as shown in FIG. 3, the probe assembly 101 may include a bridge circuit of TMR magnetoresistive sensor elements and their preamplifiers, and may be configured with a magnetic device (i.e., magnet) for magnetizing the magnetic aggregate. The up-down elastic assembly 103 may be formed of a compression spring, a bellows, or other elastic elements capable of rising and falling.
Fig. 5 is a schematic structural diagram of the test strip in one embodiment. As shown in fig. 4 to 5, the test strip 40 can be applied to the weak magnetic detection device for weak magnetic detection, and the test strip 40 can include a bottom plate 401 and a thin film 402 covering a middle portion of an upper surface of the bottom plate 401; the membrane 402 is provided with a plurality of superparamagnetic nanoparticles 408, which are solidified on the membrane 402 to form magnetic aggregates during immunoreaction, thereby facilitating quantitative detection of immunoreaction.
in one embodiment, as shown in fig. 5, an antibody (not shown) is pre-disposed on the membrane 402, and forms a magnetic aggregate of a ternary sandwich complex with a plurality of superparamagnetic nanoparticles 408 when immunoreacting with the biomolecule to be detected.
FIG. 6 is a schematic structural diagram of the test strip shown in FIG. 5 after an immunoreaction; as shown in fig. 4 to 6, the middle part of the upper surface of the bottom plate 401 is covered with a film 402, the surface of the bottom plate 401 exposed at one side of the film 402 is covered with absorbent filter paper 403, and the surface of the bottom plate 401 exposed at the other side is covered with a sample pad 404; a sample hole 405 for dripping a sample is further formed in the sample pad 404 above the bottom plate 401, and the sample pad 404 and the absorbent filter paper 403 further partially cover the side wall and the upper surface of the film 402 adjacent to the sample pad 404; the exposed surface of the membrane 402 is provided with a quality control line (i.e., C-line)407 and at least one detection line (i.e., T-line)406, and an antibody (not shown) and a plurality of superparamagnetic nanoparticles 408 are disposed on the membrane 402. Subsequently, after the sample reagent to be detected is dropped through the sample hole 405, the sample reagent to be detected moves toward the film 402 due to the adsorption force of the water-absorbing filter paper 403, and further undergoes an antigen-antibody reaction with the antibody on the film 402, and forms a ternary sandwich complex by combining with the superparamagnetic nanoparticle 408, so as to form a magnetic aggregate as shown in fig. 6 at the positions of the T-line 406 and the C-line 407. Subsequently, the test strip 40 is detected by magnetizing the magnetic aggregate and continuously measuring the intensity of a magnetic field formed after the magnetization of the magnetic aggregate, thereby realizing a chromatography immunoassay and analysis.
in one embodiment, as shown in fig. 5 to 6, the particle size of the superparamagnetic nanoparticle 408 may be 50nm to 500nm, such as 50nm, 100nm, 200nm, 300nm or 500nm and values therebetween; the specific saturation magnetization of the superparamagnetic nanoparticle 408 is greater than or equal to 10emu/g, such as 10emu/g, 20emu/g, 30emu/g, 50emu/g, and the like. The bottom plate 401 can be made of a nitrocellulose membrane or a PVDF membrane; the line width of the antibody preset on the film 402 is between 0.3mm and 1.5mm, such as 0.3mm, 0.5mm, 0.8mm, 1.0mm, 1.3mm and/or 1.5 mm; the spacing between C line 407 and T line 406 is greater than or equal to 2mm, such as 2mm, 3mm, 4mm, or 5mm, etc. In addition, according to the effective detection length of the test strip, the number of T lines embedded in advance on one test strip is smaller than a preset value, such as smaller than 10, 8, 6T lines, etc., so as to ensure the detection performance of the test strip 40.
In conclusion, the weak magnetic signal detection device provided by the invention can realize the high-sensitivity and high-precision weak magnetic detection of the magnetic aggregate on the test strip on the premise of low power consumption, has high detection and test stability and a very wide linear range, has low requirements on the processing technology, and is convenient for large-scale production and application.
the technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
the above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (11)
1. A weak magnetic signal detection device is used for detecting weak magnetic signals on a test strip and is characterized by comprising a bearing part, a support, a probe fixing plate, an upper elastic component, a lower elastic component, a left baffle, a right baffle and a floating probe, wherein a clamping groove is formed in the bearing part and used for fixing the test strip;
The probe fixing plate is arranged on the bracket through an upper elastic component and a lower elastic component, and the floating probe is fixed on the other side of the probe fixing plate, which is opposite to the upper elastic component and the lower elastic component;
The left baffle and the right baffle are respectively positioned at two opposite sides of the probe fixing plate and are fixed on the bracket, so that the translation track of the probe component is limited by the probe fixing plate;
The floating probe detects a magnetic signal on the test strip through relative translation with the test strip, and a gap between the floating probe and the test strip is within a preset distance range during the relative translation.
2. The weak magnetic signal detection device of claim 1, wherein the weak magnetic signal detection device is configured to detect a weak magnetic signal on a test strip carrying magnetic nanoparticles, and the weak magnetic signal detection device further comprises a magnet configured to generate a magnetic field for magnetizing the magnetic nanoparticles;
Wherein the floating probe is configured to detect a magnetic signal generated by the magnetic nanoparticles on the test strip after being magnetized.
3. the weak magnetic signal detection device according to claim 2, wherein the magnetic nanoparticles are superparamagnetic nanoparticles.
4. the weak magnetic signal detecting device of claim 1, wherein the floating probe further comprises at least one magneto-resistive sensor element, the at least one magneto-resistive sensor element being in elastic contact with the test strip fixed in the card slot and detecting the magnetic signal on the test strip by relative translation with the test strip.
5. The weak magnetic signal detecting device according to claim 4, wherein the at least one magnetoresistive sensor element is one or more of: tunnel magnetoresistive magnetic sensor elements, giant magnetoresistive magnetic sensor elements, anisotropic magnetoresistive sensor elements.
6. The weak magnetic signal detecting device according to claim 4, wherein the floating probe further comprises an elastic member, the at least one magneto-resistive sensor element being fixedly disposed on the elastic member;
Wherein the elastic member is in a compressed state when the magnetoresistive sensor element is in elastic contact with the test strip fixed in the card slot.
7. The weak magnetic signal detecting device according to claim 6, wherein said weak magnetic signal detecting device further comprises at least two baffles, said floating probe further comprises a probe fixing plate, said elastic component is fixedly connected with said at least one magneto-resistive sensor element through said probe fixing plate;
Wherein the at least two baffles are respectively and fixedly arranged at two sides of the probe fixing plate to limit the translation track of the probe fixing plate.
8. The weak magnetic signal detection device according to claim 1, further comprising:
the power device is connected with the bearing part through a transmission device so as to drive the test strip fixed in the clamping groove to move relative to the floating probe.
9. the weak magnetic signal detecting device according to claim 8, wherein the power device is a stepping motor, and the transmission device is a screw or a pulley;
The step motor drives the bearing part to move through the screw rod or the belt wheel so as to drive the test strip fixed in the clamping groove to move relative to the floating probe.
10. the weak magnetic signal detection device according to claim 1, wherein the card slot includes:
a base plate;
The upper cover is clamped on the bottom plate to form a cavity for fixedly placing the test strip;
Wherein, the upper cover is provided with a window for exposing the test strip fixedly placed in the cavity, so that the floating probe can pass through the window to be in elastic contact with the test strip.
11. The weak magnetic signal detection device of claim 1, wherein the test strip is a lateral immunochromatographic test strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710737948.9A CN107703297B (en) | 2017-08-24 | 2017-08-24 | Weak magnetic signal detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710737948.9A CN107703297B (en) | 2017-08-24 | 2017-08-24 | Weak magnetic signal detection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107703297A CN107703297A (en) | 2018-02-16 |
| CN107703297B true CN107703297B (en) | 2019-12-10 |
Family
ID=61169776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710737948.9A Active CN107703297B (en) | 2017-08-24 | 2017-08-24 | Weak magnetic signal detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107703297B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113219170B (en) * | 2021-04-27 | 2022-02-01 | 成都云芯医联科技有限公司 | Magnetic immunoassay system and method of use |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7323139B2 (en) * | 2002-07-26 | 2008-01-29 | Quantum Design, Inc. | Accessible assay and method of use |
| CN101571543A (en) * | 2009-04-24 | 2009-11-04 | 无锡纳生生物科技有限公司 | Magnetic nanometer immunochromatography card reading device |
| CN106970211B (en) * | 2017-03-18 | 2019-05-28 | 上海辽浦生物科技有限公司 | A kind of immune magnetic test strips experimental provision |
| CN106950362B (en) * | 2017-03-18 | 2019-07-16 | 上海叶民生物技术有限公司 | A kind of measurement method and device of immune magnetic test strips |
-
2017
- 2017-08-24 CN CN201710737948.9A patent/CN107703297B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107703297A (en) | 2018-02-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9823316B2 (en) | Magnetic biomedical sensors and sensing system for high-throughput biomolecule testing | |
| CN105628786B (en) | A kind of electromagnetic ultrasonic probe | |
| US9091720B2 (en) | Detection system using magnetic resistance sensor | |
| FI127032B (en) | Measuring arrangement, apparatus for a measuring arrangement and method for measuring a sample | |
| KR20060127918A (en) | Method and device for on-chip magnetic resonance spectroscopy | |
| US8283912B2 (en) | Sensor device with magnetic washing means | |
| KR20100054357A (en) | System for signal detection of specimen using magnetic resistance sensor | |
| CN107703297B (en) | Weak magnetic signal detection device | |
| JP4801447B2 (en) | Measuring apparatus and measuring method using magnetic sensor | |
| CN110221061B (en) | Self-positioning transmission device for magnetic chromatography chip | |
| CN113219170B (en) | Magnetic immunoassay system and method of use | |
| Yang et al. | A miniaturized giant magnetic resistance system for quantitative detection of methamphetamine | |
| KR20130061016A (en) | Apparatus for analyzing bio material using magnetic sensing method | |
| KR101214009B1 (en) | System for signal detection of specimen using magnetic resistance sensor | |
| KR101230423B1 (en) | System for signal detection of specimen using magnetic resistance sensor and Detecting Method of the same | |
| US11885800B2 (en) | Method and system for detecting analyte of interest using magnetic field sensor and magnetic particles | |
| CN107561272A (en) | Test strips, detection carry the method and device of the test strips for the aggregation that is magnetic | |
| KR20120088349A (en) | Diagnostic Equipment for signal detection of specimen using magnetic resistance sensor | |
| JP2008128677A (en) | Marker for biosensor, biosensor, and marker detection method for biosensor | |
| KR101235845B1 (en) | System for signal detection of specimen using magnetic resistance sensor and Detecting Method of the same | |
| CN211602810U (en) | Containing structure, liquid state detection device and sample analyzer | |
| JP4805631B2 (en) | Magnetic body analyzing apparatus and magnetic body analyzing method | |
| KR20090044390A (en) | System and method for signal detection of specimen using magnetic resistance sensor | |
| CN201883328U (en) | Testing device for magnetic induction intensity on fabric surface | |
| KR20120085503A (en) | Diagnostic Kit and Diagnostic Method using magnetic particles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |