CN107703297A - Weak magnetic signal detection means - Google Patents
Weak magnetic signal detection means Download PDFInfo
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- CN107703297A CN107703297A CN201710737948.9A CN201710737948A CN107703297A CN 107703297 A CN107703297 A CN 107703297A CN 201710737948 A CN201710737948 A CN 201710737948A CN 107703297 A CN107703297 A CN 107703297A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/091—Constructional adaptation of the sensor to specific applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/093—Magnetoresistive devices using multilayer structures, e.g. giant magnetoresistance sensors
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- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
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- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/096—Magnetoresistive devices anisotropic magnetoresistance sensors
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- G—PHYSICS
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- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/06—Measuring direction or magnitude of magnetic fields or magnetic flux using galvano-magnetic devices
- G01R33/09—Magnetoresistive devices
- G01R33/098—Magnetoresistive devices comprising tunnel junctions, e.g. tunnel magnetoresistance sensors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The present invention relates to weak magnetic signal detection means.A kind of weak magnetic signal detection means, available for the weak magnetic signal in test strip, the weak magnetic signal detection means includes load bearing component and floating type probe, and the load bearing component is provided with neck, and the neck is used to fix the test strips；Wherein, the floating type probe is with the test strips Elastic Contact that is fixed in the neck and by detecting the magnetic signal in the test strips with the test strips relative parallel movement.By setting floating type probe so that when carrying out weak magnetic detection to test strips using test probe, test probe Elastic Contact carries out relative motion with test strips, and then lifts the precision detected to weak magnetic signal in test strips and sensitivity.
Description
Technical field
The present invention relates to magnetic signal detection, more particularly to the device detected to the weak magnetic signal in test strips.
Background technology
At present, the weak magnetic in test strips (especially lateral immuno-chromatographic test paper strip) is entered using magnetoresistance signal detecting instrument
During row detection, due to the limitation of various component performances and technical conditions so that the sensitivity of detecting instrument and accuracy of detection etc.
Parameter can not meet the needs of current weak magnetic detection.
The content of the invention
Based on this, it is necessary to for above-mentioned technical problem, there is provided a kind of weak magnetic signal detection means, available for test strips
On weak magnetic signal detected, with lifted in test strips magnetic signal detect precision and sensitivity, with meet work as previous existence
The high request of thing sample in-vitro diagnosis.
The present invention proposes a kind of weak magnetic signal detection means, the weak magnetic signal in test strip, the weak magnetic
Signal supervisory instrument includes load bearing component and floating type probe, and the load bearing component is provided with neck, and the neck is used to fix
The test strips；
Wherein, the floating type probe with the test strips Elastic Contact that is fixed in the neck and by with
The test strips relative translation and detect the magnetic signal in the test strips.
Above-mentioned weak magnetic detection means, pass through the floating type test probe during relative translation and test strips bullet
Property contact, and then lifted in test strips weak magnetic signal detect precision and sensitivity.
In one of which embodiment, the weak magnetic signal detection means, which is used to detect, carries magnetic nanoparticle
Weak magnetic signal in test strips, the weak magnetic detection means further comprise magnet, and the magnet, which is used to produce, magnetizes the magnetic
The magnetic field of property nano particle；
Wherein, the floating type magnetic nanoparticle for being configured as detecting in the test strips of popping one's head in is being magnetized
Caused magnetic signal afterwards.
In one of which embodiment, the magnetic nanoparticle is superparamagnetic nano particle.
In one of which embodiment, the floating type probe further comprises at least one magnetoresistive sensor element,
At least one magnetoresistive sensor element with the test strips Elastic Contact that is fixed in the neck and by with it is described
The relative translation of test strips and detect the magnetic signal in the test strips.
In one of which embodiment, at least one magnetoresistive sensor element is one or more of following：
Tunnel magneto resistance magnetic sensor element, giant magnetoresistance magnetic sensor element, anisotropic magnetoresistive sensor element.
In one of which embodiment, the floating type probe can further comprise elastic parts, described at least one
Magnetoresistive sensor element is fixedly disposed on the elastic parts；
Wherein, when the magnetoresistive sensor element and the test strips Elastic Contact being fixed in the neck, institute
State elastic parts and be in compressive state.
In one of which embodiment, above-mentioned weak magnetic signal detection means can further comprise at least two baffle plates,
The floating type probe further comprises fixed plate of popping one's head in, and the elastic parts passes through the probe fixed plate and described at least one
Individual magnetoresistive sensor element is fixedly connected；
Wherein, at least two pieces of baffle plates are fixedly disposed the both sides of the probe fixed plate respectively, to limit
State the translation track of probe fixed plate.
In one of which embodiment, above-mentioned magnetic signal detection device can further comprise：
Power set, the power set are connected via transmission device and with the load bearing component, are fixed on driving
The test strips in the neck are relative to the floating type probe movement.
In one of which embodiment, the power set are stepper motors, and the transmission device is screw rod or belt wheel；
Wherein, the stepper motor moves by the screw rod or with load bearing component described in wheel drive, is fixed on driving
The test strips in the neck are relative to the floating type probe movement.
In one of which embodiment, the neck may include：
Bottom plate；
Upper lid, the upper lid is connected on the bottom plate, to be formed regularly to place the chamber of the test strips；
Wherein, the window for the test strips being fixedly positioned at for exposure in the chamber is offered on the upper lid
Mouthful, thus the floating type probe can pass through the window and with the test strips Elastic Contact.
In one of which embodiment, the test strips are lateral immuno-chromatographic test paper strips.
Brief description of the drawings
Fig. 1 is the structural representation of weak magnetic signal detection means according to an embodiment of the invention；
Fig. 2 is work shape when weak magnetic signal detection means shown in Fig. 1 carries out weak magnetic signal detection to test strips
State schematic diagram；
Fig. 3 is the structural representation of weak magnetic signal detection means according to another embodiment of the invention；
Fig. 4 is by fixed placement, the neck that carries test strips structural representation shown in Fig. 3；
Fig. 5 is the structural representation of test strips in one embodiment；
Fig. 6 is that the test strips shown in Fig. 5 carry out the structural representation after immune response.
Embodiment
In order that the purpose of the present invention, technical method and advantage are more clearly understood, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not intended to limit the scope of the present invention.
Fig. 1 is the structural representation of weak magnetic signal detection means according to an embodiment of the invention；Fig. 2 is shown in Fig. 1
The weak magnetic signal detection means gone out carries out working state schematic representation during weak magnetic signal detection to test strips.As shown in Fig. 1~2,
A kind of weak magnetic signal detection means, available for the weak magnetic signal in test strip 3, the weak magnetic signal detection means may include to hold
Carry part 1 and floating type probe 2 and neck 11；It is provided with load bearing component 1 available for the neck 11 for fixing test strips 3, float
Formula probe 2 can with the Elastic Contact of test strips 11 that is fixed in neck 11, with enable the floating type probe 2 by with test paper
Weak magnetic signal in the relatively flat in-migration test strip 3 of bar 3.Referring to Fig. 1, it can fix to carry on load bearing component 1 and be used for
The neck 11 of fixed test strips 3, floating type probe 2 neighbouring above-mentioned load bearing component 1 and can be arranged on neck 11, so as to
When carrying out weak magnetic signal detection using floating type 2 pairs of test strips 3 of probe, the floating type probe 2 can be with being fixed in neck 11
Test strips 3 Elastic Contact is kept during relative translation；Wherein, test strips 3 can be such as lateral immunity-chromatography test
The chromatograph test strip of paper etc..
Exemplarily, as shown in Fig. 1~2, after test strips 3 to be measured are fixed among neck 11, by along arrow
Shown direction adjusts the position of floating type probe 2 up and down, to cause floating type probe 2 to be contacted with test strips 3, and due to floating type
Probe 2 can carry out floating type movement in figure on above-below direction shown in arrow, and then can be by keeping floating type 2 pairs of examinations of probe
Paper slip 3 has preset elastic pressure, subsequently to cause floating type probe 2 to scheme relative to test strips 3 (or load bearing component 1) edge
The direction of arrow shown in 2 moves left and right when being detected, and keeps contacting with each other between floating type probe 2 and test strips 3.
In another embodiment, due to keep it is floating type probe 2 and test strips 3 between when carrying out relative translation all the time
Keep the technology difficulty of contact higher, the manufacturing cost of relevant device can be increased, phase can carried out during being detected
During to translation by the clearance control between floating type probe 2 and test strips 3 in default scope, such as by clearance control in milli
In meter level and its following spacing range, that is, to keep less spacing (such as less than between floating type probe 2 and test strips 3
The stability of the spacing can be kept 1mm) and effectively, this makes it possible to the precision for effectively meeting weak magnetic detection and sensitivity
Etc. demand.
Above-mentioned weak magnetic detection means, can be applied to the inspection of biomarker, environmental toxin, small molecule contaminants etc.
In survey, by setting floating type detection probe, the floating type fortune of detection probe when being detected to test strips, can be kept
It is dynamic, and then detection closely can be carried out to the magnetic field in test strips, to collect the magnetic field of the magnetic-particle of very low concentrations
Signal, and then effectively lift sensitivity and precision to the weak magnetic magnetic field detection in test strips etc..
In one embodiment, as shown in Fig. 1~2, in test strips 3 magnetic nanoparticle can be carried (in figure not show
Go out), weak magnetic detection means may include the magnet (not shown) for producing externally-applied magnetic field, and the magnet may be disposed at carrying
On part 1 and neighbouring test strips 3 fixed position, also may be disposed at the position of the neighbouring floating type test probe 2, with
Magnetic field caused by the magnet is enabled to be carried out to magnetic nanoparticle before test strips 3 are carried out with weak magnetic detection effective
Magnetization, i.e., caused magnetic after the magnetic nanoparticle that floating type test probe 2 is configured as in test strip 3 is magnetized
.The magnet can be soft magnetic bodies or permanent magnet, as long as the magnetic field such as alternating magnetic field or Constant charge soil that is, caused by the magnet
Magnetic nanoparticle can be carried out effectively magnetizing.In the present embodiment, due to using additional magnetic field can be to magnetic
Property nano particle effectively magnetized, and then can effectively lift the precision and sensitive of the weak magnetic detection subsequently carried out to it
Degree, and in order to further lift the performances such as the precision of detection and sensitivity, also test strips 3 can carried out with the mistake of weak magnetic detection
Test strips 3 are made to be in all the time in magnetic field produced by above-mentioned magnet in journey.
In one embodiment, magnetic nanoparticle can be paramagnetic nanoparticle, ferromagnetic nanoparticle or super suitable
The nano particles such as magnetic nano particle.Wherein, superparamagnetic nanoparticle generally refers to the nano-particle with magnetic responsiveness, its monocrystalline
Particle diameter is typically in below 30nm, and when the particle diameter of magnetic nano-particle is less than its superparamagnetism critical dimension, particle enters
Super-paramagnetic state.Superparamagnetic nanoparticle can be in suspended state in a liquid, and can quilt in the presence of additional gradient magnetic
Magnetize and displacement occurs, and then be easy to separate from medium in appointed part；After externally-applied magnetic field removes, its
Suspended state can be in again again, so as to have the advantages that the low and good dispersed and operability of Magnetic Isolation cost.
Just it is further detailed below by taking superparamagnetic nanoparticle as an example, i.e., when magnetic nanoparticle is received for superparamagnetic
During rice grain, superparamagnetic nano particle can effectively be magnetized in order that obtaining externally-applied magnetic field, in one embodiment,
It can keep being smaller than 0.5mm between magnet and test strips when being magnetized；For example, before weak magnetic detection is carried out and/
Or during weak magnetic detects, it is 0.2mm, 0.4mm or 0.5mm etc. to keep the spacing between magnet and test strips.
In another embodiment, test strips can carry the lateral immune chromatography test paper of superparamagnetic nano particle
Bar, and after the lateral immuno-chromatographic test paper strip carried out antigen-antibody reaction, utilize above-mentioned superparamagnetic nano particle shape
Into grappling magnetic aggregation or the magnetic aggregation of a certain ad-hoc location is fixed on, then using externally-applied magnetic field to fixed
Magnetic aggregation is magnetized, and by the magnetic signal in magnetic field produced by detecting the magnetic aggregation after magnetizing, can be completed to inspection
Survey the Qualitative and quantitative analysis of object.
In one embodiment, as shown in Fig. 1~2, above-mentioned floating type probe may include at least one magnetoresistive transducer
Element (does not indicate) in figure, and carries out relative motion relative to test strips 3 by least one magnetoresistive sensor element, comes
Realize and the weak magnetic signal of test strips 3 is detected.Above-mentioned at least one magnetoresistive element may include tunnel magneto resistance
(Tunneling Magneto Resistance, abbreviation TMR) magnetic sensing element, giant magnetoresistance (Giant Magneto
Resistance, abbreviation GMR) magnetic sensor element or anisotropic magnetoresistive (Anisotropic Magneto Resistance,
Abbreviation AMR) one or more in magnetic sensor element；The species of magnetoresistive sensor element and quantity can in i.e. floating type probe
Set according to actual demand；For example, may include four magnetic sensor elements in the floating type probe, it specifically may include one
TMR magnetic sensor elements, an AMR magnetic sensor element and two GMR magnetic sensor elements, to be sensed by combining different magnetic
The characteristic of device element lifts the sensitivity of weak magnetic signal detection means and accuracy.
In one embodiment, the magnetoresistive element in above-mentioned at least one magnetoresistive element is that TMR magnetic passes
Sensor component, i.e. the TMR magnetic sensor elements (not indicated in figure) can be with the Elastic Contacts of test strips 3 that are fixed in neck 11 simultaneously
Relative motion is carried out, with the magnetic signal in test strip 3.Because TMR magnetoresistive elements are compared to such as Hall (Hall)
Magnetoresistive element, AMR magnetoresistive elements and GMR magnetoresistive elements etc. have higher accuracy of detection and sensitivity, and
Also have the advantages that power consumption is smaller, thus using TMR magnetoresistive elements can further be lifted detection means precision and
The weak magnetic detection performance such as sensitivity.
In one embodiment, as shown in Fig. 1~2, floating type probe 2 may include elastic parts (not shown), on
At least one magnetoresistive element stated can be fixedly disposed on elastic parts, and is carrying out weak magnetic detection to test strips 3
When, magnetoresistive element compressing is may be such that on the surface of test strips 3 by extruding above-mentioned elastic parts, and elastic parts exists simultaneously
Weak magnetic can be always maintained at compressive state during detecting, and so ensure that TMR Magnetic Sensors carry out relative transport with test strips 3
Elastic Contact is kept when dynamic, and then to lift the performances such as the precision of weak magnetic detection and sensitivity.Wherein, when the magneto-resistive transducing of setting
Element for it is multiple when, can according to demand and magnetoresistive element performance parameter so that part or some magnetoresistive element
When carrying out weak magnetic detection, keep with the Elastic Contacts of test strips 3, as long as and remaining magnetic sensing element keep with test strips it
Between in the interval of setting.
In one embodiment, as shown in Fig. 1~2, in weak magnetic detection process, settable floating type probe 2 only enters
The movement in orientation above and below row, and drive load bearing component 1 to carry out left and right directions motion by setting some power set, it will float
After formula probe 2 is moved to predetermined position so that floating type probe 2 is floating type is squeezed in test strips 3 for this, and this is floated
Formula probe 2 is fixed on the translation direction such as left and right, front and rear so that floating type probe 2 only passes through elastic parts etc.
Carry out floating type up and down motion；Then, filled by using stepper motor equal power device by transmissions such as screw rod, belt wheels
Put driving load bearing component 1 to be translated, so allow for floating type probe 2 and carry out relative fortune in the horizontal direction with test strips 3
It is dynamic, and then realize and the weak magnetic of test strips 3 is detected.
In the present embodiment, it is possible to effectively avoid because it is floating type probe 2 on set Magnetic Sensor, magnetic signal acquisition, turn
Change and handle etc. relevant connection and/or equipment because floating type probe 2 frequently moves and caused by circuit, equipment damage, simultaneously
Translated by power-equipments such as stepper motors using actuator drives load bearing component 1, can effectively ensure to float
Speed and stability of relative motion between formula probe 2 and test strips 3 etc., believed with the electric current for producing relatively strong and stable
Number, while earth's magnetic field and overseas interference of the magnetic field for measurement signal effectively are avoided, and then can make further lift weak magnetic
The stability of performance and the weak magnetic detection of detection device.
Further, since there is stepper motor stride value not influenceed by various disturbing factors, error will not long term accumulation and
Control performance is good, starts, stops, upset is all the advantages that completion in a few pulses, so that utilizing in the present embodiment
Stepper motor drives the motion of test strips 3 more steady and accurate, so as to make effectively lift the property of weak magnetic detection device
Energy and the stability of weak magnetic detection.
Fig. 3 is the structural representation of weak magnetic signal detection means according to another embodiment of the invention.Such as Fig. 3 institutes
Show, below be just illustrated with a specific weak magnetic signal detection means, the weak magnetic signal detection means may include to prop up
Frame 10, the support 10 is used for the fixation of all parts in weak magnetic signal detection means, and provides one surely for weak magnetic signal detection
Fixed support and platform.Probe fixed plate 102 is arranged on by upper and lower elastic parts 103 on above-mentioned support 10, and the probe
The opposite side that fixed plate 102 is relatively set with elastic parts 103 is fixed with the interior probe component 101 for setting TMR magnetic sensor elements,
Right baffle-plate 104 and right shell body 105 positioned at the relative both sides of probe fixed plate 102 and are each attached on support 10 respectively, to pass through
Probe fixed plate 102 come limit probe component 101 translation track so that its one guarantee kinematic accuracy space in move.
The translatable lower section for being arranged at above-mentioned probe component 101 of sliding block 203 for carrying test strips, it is contained with the neck of test strips
30 are fastened in the clamping groove (not shown) opened up on sliding block 203, to cause TMR Magnetic Sensors can be by window 301
Weak magnetic signal detection can be carried out to the test strips being contained in neck 30.Stepper motor 201 then passes through screw rod 202 and sliding block
203 connections, to be translated by the screw rod 202 with movable slider 203, and then cause the TMR magnetic being arranged in probe component 101
The relative test strips being contained in neck 30 of sensor carry out quick uniform motion, so as to realizing to the high accuracy of test strips and
Highly sensitive weak magnetic detection.
Fig. 4 is by fixed placement, the neck that carries test strips structural representation shown in Fig. 3.Such as Fig. 4 institutes
Show, neck 30 may include bottom plate 307 and be fastened on the upper lid 302 of the bottom plate 307, be offered on this on lid 302 for being added dropwise
The well 303 of test sample, the locating notch 304 for carrying out test position fix, the guide angle 305 for test guiding
Deng being additionally provided with guiding circular arc 306 for guiding on the side top that bottom plate 307 exposes, and the upper surface of upper lid 302 opens up
Window 301 is used for the detection part for exposing test strips 40 of the fixed placement in the neck 30, i.e., above-mentioned probe component 101 is logical
Cross the weak magnetic signal detection deeply realized into the window 301 to test strips 40.
As shown in figs. 34, in one embodiment, stepper motor 201 can drive screw rod 202 to rotate, so as to band
Movable slider 203 makees translational motion, such as moves forward and backward；The box thread corresponding with screw rod 202 is may be provided with sliding block 203, together
When sample notch (not shown) for neck 30 can be inserted can be also offered at the top of sliding block 203.Probe component 101
With probe fixed plate 102 and up and down the grade composition of elastic parts 103 can fluctuate formula motion part.Carried out to test strips 40
Before detection, TMR Magnetic Sensors in probe component 101 are first close to the surface of test strips 40, once test strips 40 pass with respect to TMR magnetic
Sensor moves forwards or backwards, and the projection of the probe of the TMR Magnetic Sensors will be adopted in the groove of test strips 40
Sample, due to now have in the groove of test strips 40 particles with superparamagnetism form magnetization after magnetic aggregation, TMR Magnetic Sensors
Probe by cutting the magnetic line of force in magnetic field caused by magnetic aggregation after magnetization, just have corresponding electric signal and produce, enter
And realize and the weak magnetic of test strips 40 is detected.
In another embodiment, as shown in figure 3, probe component 101 may include the electricity that TMR magnetoresistive sensor elements are formed
Bridge circuit and its preamplifier, it can also configure a magnetic device (i.e. magnet) to magnetized magnetic aggregation.Upper and lower bullet
Property component 103 can by compression spring, bellows etc. it is other can rise dipping and heavings flexible members form.
Fig. 5 is the structural representation of test strips in one embodiment.As shown in Figures 4 and 5, test strips 40 can be applied to above-mentioned
Weak magnetic detection means carries out weak magnetic detection, and the test strips 40 may include bottom plate 401 and be covered in the upper surface pars intermedia of bottom plate 401
The film 402 divided；Wherein, some superparamagnetic nano particles 408 are provided with film 402, for consolidating during immune response
Change and magnetic aggregation is formed on film 402, and then be easy to carry out immune response quantitative detection.
In one of which embodiment, as shown in figure 5, presetting antibody (not shown) on film 402, this is anti-
Body is compound with forming ternary sandwich during biomolecule to be detected generation immune response together with some superparamagnetic nano particles 408
The magnetic aggregation of thing.
Fig. 6 is that the test strips shown in Fig. 5 carry out the structural representation after immune response；As shown in figures 4-6, bottom plate 401
Upper surface center section covered with film 402, filtered on the surface of the bottom plate 401 of the side of film 402 exposure covered with water suction
Paper 403, opposite side exposure bottom plate 401 surface on then covered with sample pad 404；Sample pad 404 above bottom plate 401 is also
Offer the sample well 405 for sample to be added dropwise, and sample pad 404 also partly covered respectively with absorbent filter 403 it is its immediate
Film 402 side wall and upper surface；The surface that film 402 exposes is provided with nature controlling line (i.e. C-line) 407 and at least one
Detection line (i.e. T-line) 406, while antibody (not shown) and some super-paramagnetism nanos are additionally provided with film 402
Particle 408.Subsequently, by sample well 405 be added dropwise testing sample reagent after, due to the absorption affinity of absorbent filter 403 cause it is to be measured
Sample reagent moves to the direction of film 402, and then antigen-antibody reaction occurs with the antibody on film 402, and combines superparamagnetic
Property nano particle 408 formed ternary sandwich complex, gathered with forming magnetic as shown in Figure 6 in T lines 406 and the opening position of C lines 407
Collect thing.Subsequently when being detected to test strips 40 by magnetized magnetic aggregation, and continue after measuring magnetic aggregation solid magnetizing
The power in formed magnetic field, and then realize chromatography immunization experiment and analysis.
In one embodiment, as shown in Fig. 5~6, the particle diameter of above-mentioned superparamagnetic nano particle 408 can be 50nm~
500nm, as 50nm, 100nm, 200nm, 300nm or 500nm and its between numerical value；The ratio of superparamagnetic nano particle 408 is satisfied
It is more than or equal to 10emu/g, such as 10emu/g, 20emu/g, 30emu/g, 50emu/g with the intensity of magnetization.The material of bottom plate 401
Can be nitrocellulose filter or pvdf membrane；The line width of preset antibody is between 0.3~1.5mm on film 402, such as
0.3mm, 0.5mm, 0.8mm, 1.0mm, 1.3mm and/or 1.5mm are equivalent；Spacing between C lines 407 and T lines 406 be greater than or
Equal to 2mm, as 2mm, 3mm, 4mm or 5mm are equivalent.In addition, can be according to the effective detection length of test strips, in a test strips
The T line number amounts embedded in advance are less than default value, such as less than 10,8,6 T lines etc., to ensure the detection of test strips 40
Performance.
To sum up, weak magnetic signal detection means provided by the present invention, can be realized on the premise of low-power consumption to test strips
On magnetic aggregation carry out the weak magnetic detection of high sensitivity and pinpoint accuracy, and it is high to detect measuring stability, has very wide
The range of linearity, it is relatively low simultaneously for the technological requirement of processing, it is convenient for large-scale production application.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (11)
1. a kind of weak magnetic signal detection means, the weak magnetic signal in test strip, it is characterised in that the weak magnetic signal
Detection means includes load bearing component and floating type probe, and the load bearing component is provided with neck, and the neck is used for described in fixation
Test strips；
Wherein, the floating type probe with the test strips Elastic Contact that is fixed in the neck and by with it is described
Test strips relative translation and detect the magnetic signal in the test strips.
2. weak magnetic signal detection means according to claim 1, it is characterised in that the weak magnetic signal detection means is used for
Detection carries the weak magnetic signal in the test strips of magnetic nanoparticle, and the weak magnetic detection means further comprises magnet, institute
State magnet be used for produce the magnetization magnetic nanoparticle magnetic field；
Wherein, the floating type probe is configured as detecting the magnetic nanoparticle in the test strips institute after being magnetized
Caused magnetic signal.
3. weak magnetic signal detection means according to claim 2, it is characterised in that the magnetic nanoparticle is superparamagnetic
Property nano particle.
4. weak magnetic signal detection means according to claim 1, it is characterised in that the floating type probe further comprises
At least one magnetoresistive sensor element, at least one magnetoresistive sensor element and the test paper being fixed in the neck
Bar Elastic Contact simultaneously detects the magnetic signal in the test strips by the relative translation with the test strips.
5. weak magnetic signal detection means according to claim 4, it is characterised in that at least one magnetoresistive transducer member
Part is one or more of following：Tunnel magneto resistance magnetic sensor element, giant magnetoresistance magnetic sensor element, anisotropic magnetoresistive
Sensor element.
6. weak magnetic signal detection means according to claim 4, it is characterised in that the floating type probe further comprises
Elastic parts, at least one magnetoresistive sensor element are fixedly disposed on the elastic parts；
Wherein, when the magnetoresistive sensor element and the test strips Elastic Contact being fixed in the neck, the bullet
Property component is in compressive state.
7. weak magnetic signal detection means according to claim 6, it is characterised in that the weak magnetic signal detection means enters one
Step includes at least two baffle plates, and the floating type probe further comprises fixed plate of popping one's head in, and the elastic parts passes through the spy
Head fixed plate is fixedly connected with least one magnetoresistive sensor element；
Wherein, at least two baffle plate is fixedly disposed the both sides of the probe fixed plate respectively, to limit the spy
The translation track of head fixed plate.
8. weak magnetic signal detection means according to claim 1, it is characterised in that the weak magnetic signal detection means enters one
Step includes：
Power set, the power set are connected via transmission device and with the load bearing component, are fixed on drive described
The test strips in neck are relative to the floating type probe movement.
9. weak magnetic signal detection means according to claim 8, it is characterised in that the power set are stepper motors,
The transmission device is screw rod or belt wheel；
Wherein, the stepper motor moves by the screw rod or with load bearing component described in wheel drive, is fixed on drive described
The test strips in neck are relative to the floating type probe movement.
10. weak magnetic signal detection means according to claim 1, it is characterised in that the neck includes：
Bottom plate；
Upper lid, the upper lid is connected on the bottom plate, to be formed regularly to place the chamber of the test strips；
Wherein, the window for the test strips being fixedly positioned at for exposure in the chamber is offered on the upper lid, with
Cause the floating type probe can pass through the window and with the test strips Elastic Contact.
11. weak magnetic signal detection means according to claim 1, it is characterised in that the test strips are that layer is laterally immunized
Analyse test strips.
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| CN201710737948.9A CN107703297B (en) | 2017-08-24 | 2017-08-24 | Weak magnetic signal detection device |
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|---|---|---|---|
| CN201710737948.9A CN107703297B (en) | 2017-08-24 | 2017-08-24 | Weak magnetic signal detection device |
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| CN107703297B CN107703297B (en) | 2019-12-10 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113219170A (en) * | 2021-04-27 | 2021-08-06 | 成都云芯医联科技有限公司 | Magnetic immunoassay system and method of use |
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