CN107691854B - Pet eye disease ointment and preparation method thereof - Google Patents

Abstract

The pet eye disease ointment and the preparation method thereof comprise the following components by mass percent: 0.1-3% of chrysanthemum extract, 0.5-4% of honeysuckle extract, 0.1-2% of cortex mori extract, 0.1-3% of angelica extract, 0.1-3% of licorice extract, 0.5-3% of astragalus extract, 0.1-3% of rhizoma anemarrhenae extract, 0.1-3% of dandelion extract, 0.1-3% of cimicifugae foetidae extract, 3-12% of white sugar, 1-10% of milk powder, 8-16% of bovine hydrolyzed cream, 8-20% of animal oil, 3-13% of chicken liver powder, 0.5-3% of sodium carboxymethylcellulose, 0.1-0.35% of sodium benzoate, 0.00025% of vitamin A, 0.005% of vitamin B1, 0.005% of vitamin B2, 0.0025% of vitamin B6, 0.00005% of vitamin B12, 0.05% of vitamin E, 0.5% of taurine and the balance of water. The pet eye disease ointment has the functions of helping dogs and cats to eliminate eye inflammation and quickening tissue repair of cornea and conjunctiva.

Description

Pet eye disease ointment and preparation method thereof

Technical Field

The invention relates to a functional health care product for pets, in particular to an eye disease ointment for pets.

Background

The eyes of dogs and cats are specially constructed to make them see in the dark, and dogs and cats are also said to be visually active animals. However, the eyes of the dog or cat are very delicate organs which are also fragile and dull due to external infection, so that the dog or cat needs to be hospitalized as soon as possible to avoid irreparable injury in normal times with any slight symptoms.

The current pet market is a product of eye drops for helping dogs and cats to eliminate eye inflammation, the operation of helping dogs and cats to use the eye drops is difficult to be executed for pets, most dogs and cats have a resistance attitude for using the eye drops, and no edible health care product for helping dogs and cats to eliminate the eye inflammation and quicken the tissue repair of cornea and conjunctiva exists in the current market.

Disclosure of Invention

In order to solve one or more of the problems, the invention provides the pet eye disease ointment and the preparation method thereof.

According to one aspect of the invention, the invention provides an eye disease ointment for pets, which comprises the following components in percentage by mass: 0.1-3% of chrysanthemum extract, 0.5-4% of honeysuckle extract, 0.1-2% of cortex mori extract, 0.1-3% of angelica extract, 0.1-3% of licorice extract, 0.5-3% of astragalus extract, 0.1-3% of rhizoma anemarrhenae extract, 0.1-3% of dandelion extract, 0.1-3% of cimicifugae foetidae extract, 3-12% of white sugar, 1-10% of milk powder, 8-16% of bovine hydrolyzed cream, 8-20% of animal oil, 3-13% of chicken liver powder, 0.5-3% of sodium carboxymethylcellulose, 0.1-0.35% of sodium benzoate, 0.00025% of vitamin A, 0.005% of vitamin B1, 0.005% of vitamin B2, 0.0025% of vitamin B6, 0.00005% of vitamin B12, 0.05% of vitamin E, 0.5% of taurine and the balance of water.

In some embodiments, wherein:

1% of chrysanthemum extract, 1.5% of honeysuckle extract, 0.5% of cortex mori extract, 1% of angelica extract, 1% of liquorice extract, 1.5% of astragalus extract, 1% of rhizoma anemarrhenae extract, 1% of dandelion extract, 1% of cimicifugae foetidae extract, 7% of white sugar, 6% of milk powder, 10% of bovine hydrolyzed cream, 15% of animal oil, 8% of chicken liver powder, 2% of sodium carboxymethylcellulose, 0.15% of sodium benzoate and 41.7872% of water.

According to another aspect of the present invention, there is provided a method for preparing the above-mentioned eye disease ointment for pets, comprising the steps of,

(1) weighing flos Chrysanthemi extract, flos Lonicerae extract, cortex Mori extract, radix Angelicae sinensis extract, Glycyrrhrizae radix extract, radix astragali extract, rhizoma anemarrhenae extract, herba Taraxaci extract, cimicifugae rhizoma extract, white sugar, milk powder, adeps bovis seu Bubali hydrolysate, animal oil, and chicken liver powder according to formula proportion, adding water according to formula proportion, homogenizing, and stirring to obtain slurry; weighing sodium carboxymethylcellulose according to a formula proportion and adding the sodium carboxymethylcellulose into a mixing tank;

(2) starting a steam inlet switch of the batching tank, heating the product obtained in the step (1) to 70-80 ℃ while stirring, and preserving heat;

(3) stirring and boiling the product obtained in the step (2), sequentially adding the vitamin A, the vitamin B1, the vitamin B2, the vitamin B6, the vitamin B12, the vitamin E, the taurine and the sodium benzoate weighed according to the formula proportion into a mixing tank, and continuously boiling for 20-30 minutes;

(4) and (4) cooling the mixture obtained in the step (3) to obtain a final product.

In some embodiments, the dosing tank is a steam heated dosing tank.

In some embodiments, the vapor pressure in the dosing tanks in steps (2) and (3) is from 0.2 to 0.4 MPa.

Compared with the existing pet eye disease health care product, the eye disease ointment of the invention has the following beneficial effects:

chrysanthemum: has anti-inflammatory effect, contains flavonoids as main active ingredients, and can directly eliminate free radicals to exert antioxidant activity, thereby reducing damage of free radicals to cells and tissues.

Honeysuckle flower: the honeysuckle flower health tea is sweet in taste and cold in nature, has the effects of clearing away heat and toxic materials, dispelling wind and heat, resisting inflammation and disinfecting, wherein chlorogenic acid and swertiamarin are main anti-inflammatory substances in honeysuckle flower.

White mulberry root-bark: the mulberry bark decoction has the bacteriostatic action, and the flavonoid compound is the main material basis of the bacteriostatic activity.

Chinese angelica: has anti-inflammatory effect.

Licorice root: has effects in invigorating spleen, replenishing qi, clearing away heat and toxic materials, relieving pain, and regulating drug properties, and the flavone of Glycyrrhrizae radix has antiinflammatory effect.

Astragalus root: polysaccharides, saponins and flavonoids compounds in the astragalus can enable the astragalus to play a role in regulating immunity in two directions, enhance the immunity of cells and organisms and play a good anti-infection role.

Rhizoma anemarrhenae: bitter, sweet and cold; clearing heat and purging fire; pharmacological research shows that the active ingredients of the rhizoma anemarrhenae mainly comprise steroidal saponin compounds and flavonoid compounds, so that the rhizoma anemarrhenae has anti-inflammatory and antibacterial effects.

Dandelion: sweet, slightly bitter and cold, clearing away heat and toxic materials; has broad-spectrum antibacterial, antiinflammatory and antioxidant effects.

Cimicifugae foetidae: pungent, slightly sweet and slightly cold; clearing away heat and toxic material, and the phenolic acid compounds in cimicifuga rhizome have antibacterial and antiinflammatory effects.

Vitamin A: also known as retinol; can be used for preventing nyctalopia and visual deterioration, and treating various eye diseases such as xerophthalmia, keratomalacia and nyctalopia (vitamin A can promote the formation of photosensitive pigment in eyes).

B vitamins (vitamin B1, vitamin B2, vitamin B6, vitamin B12): is related to the health of optic nerve and also has the function of protecting cornea. Lack of B vitamins, easy occurrence of neuropathy, neuritis, photophobia, blurred vision, lacrimation and the like

Vitamin E: antioxidant effect, recently it has also been found that vitamin E inhibits the lipid peroxidation reaction in the lens of the eye, dilates peripheral blood vessels, improves blood circulation, prevents the occurrence and development of taurine in myopic eyes: taurine is a sulfur-containing amino acid, the major amino acid in mature retina.

The pet eye disease ointment can promote the growth and development of retina, relieve ciliary muscle spasm, and competitively combine taurine with reducing sugar in aqueous humor and vitreous humor to prevent protein in vitreous humor from being saccharified and oxidized. The ingredients are added into the eye disease ointment in a certain proportion, and the eye disease ointment has the functions of helping dogs and cats to eliminate eye inflammation and quickening tissue repair of cornea and conjunctiva.

Detailed Description

The present invention will be described in further detail with reference to examples.

Preparation of eye disease ointment

Example 1:

the total amount of the raw materials is 100 kg, wherein the total amount of the raw materials is 0.1 kg of chrysanthemum extract, 0.5 kg of honeysuckle extract, 0.1 kg of cortex mori extract, 0.1 kg of angelica extract, 0.1 kg of liquorice extract, 0.5 kg of astragalus extract, 0.1 kg of rhizoma anemarrhenae extract, 0.1 kg of dandelion extract, 0.1 kg of cimicifugae foetidae extract, 3 kg of white sugar, 10 kg of milk powder, 16 kg of bovine hydrolysis cream, 20 kg of animal oil, 13 kg of chicken liver powder, 0.5 kg of sodium carboxymethylcellulose, 0.15 kg of sodium benzoate, 0.00025 kg of vitamin A, 10.005 kg of vitamin B, 20.005 kg of vitamin B, 60.0025 kg of vitamin B, 120.00005 kg of vitamin E, 0.05 kg of taurine and 35.0872 kg of water.

The preparation process comprises the following steps:

(1) weighing flos Chrysanthemi extract, flos Lonicerae extract, cortex Mori extract, radix Angelicae sinensis extract, Glycyrrhrizae radix extract, radix astragali extract, rhizoma anemarrhenae extract, herba Taraxaci extract, cimicifugae rhizoma extract, white sugar, milk powder, adeps bovis seu Bubali hydrolysate, animal oil, and chicken liver powder according to formula proportion, adding water according to formula proportion, homogenizing, and stirring to obtain slurry; weighing sodium carboxymethylcellulose according to a formula proportion and adding the sodium carboxymethylcellulose into a mixing tank;

(2) starting a steam inlet switch of the batching tank, and heating the product obtained in the step (1) to 70-80 ℃ while stirring; the steam pressure in the batching tank is 0.2-0.4 MPa;

(3) stirring and boiling the product obtained in the step (2), sequentially adding the vitamins, taurine and sodium benzoate weighed according to the formula proportion into a mixing tank, and continuously boiling for 20-30 minutes;

(4) and (4) cooling the mixture obtained in the step (3) to obtain a final product.

Example 2:

the total amount of the raw materials is 100 kg, wherein 1 kg of chrysanthemum extract, 1.5 kg of honeysuckle extract, 0.5 kg of cortex mori extract, 1 kg of angelica extract, 1 kg of licorice extract, 1.5 kg of astragalus extract, 1 kg of rhizoma anemarrhenae extract, 1 kg of dandelion extract, 1 kg of rhizoma cimicifugae extract, 7 kg of white sugar, 6 kg of milk powder, 10 kg of bovine hydrolytic cream, 15 kg of animal oil, 8 kg of chicken liver powder, 2 kg of sodium carboxymethylcellulose, 0.15 kg of sodium benzoate, 0.00025 kg of vitamin A, 10.005 kg of vitamin B, 20.005 kg of vitamin B, 60.0025 kg of vitamin B, 120.00005 kg of vitamin B, 0.05 kg of vitamin E, 0.5 kg of taurine and 41.7872 kg of water.

The preparation process is the same as in example 1.

Example 3:

the total amount of the raw materials is 100 kg, wherein 3 kg of chrysanthemum extract, 4 kg of honeysuckle extract, 2 kg of cortex mori extract, 3 kg of angelica extract, 3 kg of liquorice extract, 3 kg of astragalus extract, 3 kg of rhizoma anemarrhenae extract, 3 kg of dandelion extract, 3 kg of rhizoma cimicifugae extract, 12 kg of white sugar, 1 kg of milk powder, 8 kg of bovine hydrolysis cream, 8 kg of animal oil, 3 kg of chicken liver powder, 3 kg of sodium carboxymethylcellulose, 0.15 kg of sodium benzoate, 0.00025 kg of vitamin A, 10.005 kg of vitamin B, 20.005 kg of vitamin B, 60.0025 kg of vitamin B, 120.00005 kg of vitamin B, 0.05 kg of vitamin E, 0.5 kg of taurine and 37.2872 kg of water.

The preparation process is the same as in example 1.

The components mentioned in examples 1 to 3 above are commercially available, wherein the components mentioned in relation to the extract are provided by biosciences of Shengmeikang, Beijing, in a ratio of 10: 1. Wherein the animal oil is fish oil.

Clinical effect test of eye disease ointment

In real life, many pets suffer from some eye diseases. The eye disease ointment is a functional health product which can effectively help dogs and cats to reduce eye inflammation and accelerate tissue repair of cornea and conjunctiva. The test takes the selected dog with bacterial infection of eyes as a test object, and the clinical curative effect of the eye disease ointment is analyzed and compared.

1. Test animals: 90 dogs (1-3 years old) with bacterial eye disease were selected and were normal except for the eye where disease was a problem.

2. And (3) testing the sample: three kinds of edible pastes were prepared according to the eye disease ointment formulations of example 1, example 2 and example 3, respectively.

3. Grouping: randomly selecting dogs with bacterial eye diseases for grouping, wherein a control group, namely a group without being fed with eye disease ointment, is set as 15 dogs; the positive drug group is fed with 15 levofloxacin tablets with normal dose, the three scheme groups are fed with 15 eye disease ointment groups prepared by three groups of examples, and another test group is fed with drug and one example ointment at random by each time of feeding, and the following table 1 shows. The food (basic ration produced by the same manufacturer) and the drinking water are freely taken in the same living environment. Each group was evaluated after 2 weeks.

TABLE 1 test grouping

4. Evaluation of Effect

4.1 assessment of ocular signs: the slit lamp microscope was used to observe eye signs at the beginning of the experiment. The different degrees of 4 indexes of conjunctival edema, conjunctival congestion, eyelid edema and secretion generation are respectively scored by a blind method (0-3 points). Finally, the scores of the 4 indexes are added to obtain a total score, wherein the total score is the lowest score of 0 and the maximum score of 12. The test was carried out 2 weeks later and evaluated again, and the results of the two tests were compared and analyzed. Statistical analysis was performed using the SPSS20.0 software, and all results are expressed as means. + -. standard deviation (x. + -.s), and the scoring criteria are shown in Table 2.

TABLE 2 Ocular sign score criteria

4.2 blood inflammatory cell indices: at the beginning of the test, blood was collected from each group of dogs, and the amount of leukocytes in the blood was measured by an automatic blood cell count analyzer. And collecting and measuring after 2 weeks of the test, and comparing and analyzing the results of the previous and next times.

5. Results

5.1 Ocular sign

As shown in Table 3, after two weeks, the index of decrease in ocular signs was significantly higher in the groups of example 1, example 2 and example 3 than in the normal control group (with uniform variance, p < 0.05), whereas the index of decrease in ocular signs was relatively higher in example 2 but lower than in the positive drug group, indicating that the eye ointment had a certain improvement effect on ocular signs, and the effect of example 2 was the best. The ointment and medicine group is obviously higher than the three example groups and the positive medicine group (with uniform variance, p is less than 0.05), which indicates that the eye disease ointment can obviously relieve the symptoms of eye diseases when being used together with antibiotic medicines.

Table 3 ocular signs before and after the test (x ± s, n ═ 15)

Note: p < 0.05, p < 0.01, as follows.

5.2 blood inflammatory cell conditions

As shown in Table 4, the number of leukocytes in each test group is better than that in the normal control group (with uniform variance and P < 0.05) after two weeks, which indicates that the administration of the eye disease ointment can significantly reduce the number of leukocytes and relieve inflammatory reaction, and the effect is better when the eye disease ointment is used together with antibiotic drugs.

TABLE 4 leukocyte counts before and after the experiment (10)⁹/L，x±s，n＝15)

Note: the normal white blood cell number of the dogs ranges from 6 to 17 x 10⁹/L

In conclusion, the eye disease ointment has the function of improving the adverse symptoms of the eyes, is beneficial to the repair of tissues of cornea and conjunctiva, and can reduce inflammatory reaction; wherein example 2 is to achieve an optimal formulation. If the eye drop is matched with eye antibiotic medicines, the effect is better.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the inventive concept thereof, and these changes and modifications can be made without departing from the spirit and scope of the invention.

Claims (4)

1. The pet eye disease ointment is characterized by comprising the following components in percentage by mass:

0.1-3% of chrysanthemum extract, 0.5-4% of honeysuckle extract, 0.1-2% of cortex mori extract, 0.1-3% of angelica extract, 0.1-3% of licorice extract, 0.5-3% of astragalus extract, 0.1-3% of rhizoma anemarrhenae extract, 0.1-3% of dandelion extract, 0.1-3% of cimicifugae foetidae extract, 3-12% of white sugar, 1-10% of milk powder, 8-16% of bovine hydrolyzed cream, 8-20% of animal oil, 3-13% of chicken liver powder, 0.5-3% of sodium carboxymethylcellulose, 0.1-0.35% of sodium benzoate, 0.00025% of vitamin A, 0.005% of vitamin B1, 0.005% of vitamin B2, 0.0025% of vitamin B6, 0.00005% of vitamin B12, 0.05% of vitamin E, 0.5% of taurine and the balance of water;

the preparation method comprises the following steps:

(1) weighing flos Chrysanthemi extract, flos Lonicerae extract, cortex Mori extract, radix Angelicae sinensis extract, Glycyrrhrizae radix extract, radix astragali extract, rhizoma anemarrhenae extract, herba Taraxaci extract, cimicifugae rhizoma extract, white sugar, milk powder, adeps bovis seu Bubali hydrolysate, animal oil, and chicken liver powder according to formula proportion, adding water according to formula proportion, homogenizing, and stirring to obtain slurry; weighing sodium carboxymethylcellulose according to a formula proportion and adding the sodium carboxymethylcellulose into a mixing tank;

(2) starting a steam inlet switch of the batching tank, heating the product obtained in the step (1) to 70-80 ℃ while stirring, and preserving heat;

(3) stirring and boiling the product obtained in the step (2), sequentially adding the vitamin A, the vitamin B1, the vitamin B2, the vitamin B6, the vitamin B12, the vitamin E, the taurine and the sodium benzoate weighed according to the formula proportion into a mixing tank, and continuously boiling for 20-30 minutes;

(4) and (4) cooling the mixture obtained in the step (3) to obtain a final product.

2. The ophthalmic disease ointment for pets as set forth in claim 1, wherein:

1% of chrysanthemum extract, 1.5% of honeysuckle extract, 0.5% of cortex mori extract, 1% of angelica extract, 1% of liquorice extract, 1.5% of astragalus extract, 1% of rhizoma anemarrhenae extract, 1% of dandelion extract, 1% of cimicifugae foetidae extract, 7% of white sugar, 6% of milk powder, 10% of bovine hydrolyzed cream, 15% of animal oil, 8% of chicken liver powder, 2% of sodium carboxymethylcellulose, 0.15% of sodium benzoate and 41.7872% of water.

3. The ophthalmic disease ointment for pet of claim 1, wherein the dispensing tank is a steam heating dispensing tank.

4. The ophthalmic disease ointment for pet as claimed in claim 3, wherein the vapor pressure in the dispensing tank in the steps (2) and (3) is 0.2-0.4 MPa.