CN107691246A - Chronic intermittent hypoxia experimental animal system and its control method - Google Patents
Chronic intermittent hypoxia experimental animal system and its control method Download PDFInfo
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- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 86
- 230000007954 hypoxia Effects 0.000 title claims abstract description 82
- 230000001684 chronic effect Effects 0.000 title claims abstract description 31
- 238000010171 animal model Methods 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 94
- 239000001301 oxygen Substances 0.000 claims abstract description 94
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 94
- 241001465754 Metazoa Species 0.000 claims abstract description 79
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 72
- 239000007789 gas Substances 0.000 claims abstract description 48
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 36
- 238000012545 processing Methods 0.000 claims abstract description 13
- 239000008280 blood Substances 0.000 claims description 20
- 210000004369 blood Anatomy 0.000 claims description 20
- 238000007405 data analysis Methods 0.000 claims description 9
- 230000000052 comparative effect Effects 0.000 claims description 8
- 230000009466 transformation Effects 0.000 claims description 7
- 238000007781 pre-processing Methods 0.000 claims description 5
- 230000000241 respiratory effect Effects 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims 1
- 229910001882 dioxygen Inorganic materials 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 9
- 241000699670 Mus sp. Species 0.000 abstract description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 230000007958 sleep Effects 0.000 description 6
- 230000029058 respiratory gaseous exchange Effects 0.000 description 5
- 208000008784 apnea Diseases 0.000 description 4
- 238000004891 communication Methods 0.000 description 4
- 208000018875 hypoxemia Diseases 0.000 description 4
- 230000000306 recurrent effect Effects 0.000 description 3
- 230000004099 anaerobic respiration Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000001991 pathophysiological effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 201000002859 sleep apnea Diseases 0.000 description 2
- 101100460719 Mus musculus Noto gene Proteins 0.000 description 1
- 206010041349 Somnolence Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000008246 gaseous mixture Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000414 obstructive effect Effects 0.000 description 1
- 208000001797 obstructive sleep apnea Diseases 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/0047—Air-conditioning, e.g. ventilation, of animal housings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D11/00—Control of flow ratio
- G05D11/02—Controlling ratio of two or more flows of fluid or fluent material
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
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Abstract
A kind of chronic intermittent hypoxia experimental animal system disclosed by the invention, including input equipment, nitrogen feedway, air feeder, acquisition module, controller and mixed gas discharger, the acquisition module is used for the vital sign data for gathering gas content and animal in rearing-box；The controller is used for processing data；According to circulation intermittent hypoxia control data control nitrogen feedway and air feeder supply；The data and the animal eupnea oxygen concentration threshold value and vital signs threshold values of input gathered according to acquisition module control air feeder and nitrogen feedway to supply more afterwards.Monitor whether animal is in anaerobic environment and the vital sign in anaerobic environment in real time by acquisition module, it was found that animal life sign interrupts the programme-control of default circulation intermittent hypoxia control data in time extremely, air is passed through into rearing-box in time, greatly reduce the unusual death rate of experiment mice, experiment fees are saved, increase the practicality and science of system.
Description
Technical field
The present invention relates to experimental system technical field, and in particular to a kind of chronic intermittent hypoxia experimental animal system and its control
Method processed.
Background technology
Chronic intermittent hypoxia is the main pathophysiological basis of OSAS, and obstructive sleep is exhaled
Inhale that pause syndrome refers in sleep procedure every night apnea recurrent exerbation more than 30 times or sleep apnea low refers to
Count time/hour of (AHI) >=5 and with the clinical symptoms such as drowsiness.Apnea refers to that mouth and nose respiratory air flow is complete in sleep procedure
Stop more than 10 seconds；Low pass gas refers to that respiratory air flow intensity (amplitude) is compared with foundation level reduction by more than 50% in sleep procedure, and
It is awake compared with foundation level decline >=4% or micro- with blood oxygen saturation.OSAS causes heart and brain blood
The main pathophysiological basis of pipe complication is chronic intermittent hypoxia, thus prepare chronic intermittent hypoxia experimental animal model for
Treatment OSAS and caused angiocardiopathy have important scientific research value.
The content of the invention
For in the prior art the defects of, an object of the present invention is to provide chronic intermittent hypoxia experimental animal system
System, save experiment fees, the practicality of system and scientific height.
In a first aspect, chronic intermittent hypoxia experimental animal system provided by the invention, including input equipment, nitrogen supply dress
Put, air feeder, acquisition module, controller and mixed gas discharger, the input equipment are used to input between circulation
Control data, animal eupnea oxygen concentration threshold value and the vital signs threshold values of breakthrough oxygen；The nitrogen feedway is used for root
Nitrogen is supplied according to circulation intermittent hypoxia control data supply nitrogen and according to controller control instruction；The air feeder is used
According to circulation intermittent hypoxia control data supply air and according to controller control instruction supply air；The acquisition module is used
In the vital sign data for gathering gas content and animal in rearing-box；The controller is used for processing data；According to circulation
Intermittent hypoxia control data controls nitrogen feedway and air feeder supply；The data and defeated gathered according to acquisition module
The animal eupnea oxygen concentration threshold value and vital signs threshold values entered controls air feeder and nitrogen feedway more afterwards
Supply；The mixed gas discharger is used to exclude the mixed gas in rearing-box.
Alternatively, the acquisition module includes oxygen concentration sensor, and the oxygen concentration sensor, which is used to gather, raises
Oxygen content in case.
Alternatively, the acquisition module also includes electrocardiogram acquisition module and blood oxygen acquisition module, the electrocardiogram acquisition module
For gathering the electrocardiogram (ECG) data of animal in rearing-box；The blood oxygen acquisition module is used for the blood oxygen saturation for gathering animal in rearing-box
Degrees of data.
Alternatively, during the supply for the gas with various that the control data of the circulation intermittent hypoxia includes circulation intermittent hypoxia
Between, gas with various supply the cycle and rearing-box in oxygen concentration.
Alternatively, the system also includes warning device, and the controller is additionally operable to the raising gathered according to acquisition module
Animal life sign and the life of input of the oxygen concentration compared with the oxygen concentration threshold value inputted with acquisition module collection in case
Life signs threshold values are compared to obtain comparative result, when comparative result exception, warning device alert.
Alternatively, the controller includes data pre-processing unit, data analysis unit and control instruction generation unit, institute
Data pre-processing unit is stated to be used to be pre-processed according to the data that acquisition module gathers；The data analysis unit is used for basis
The data of acquisition module collection are analyzed with the oxygen concentration threshold value and vital signs threshold values of the animal eupnea of input,
Obtain the result whether animal is in anaerobic environment；The control instruction generation unit is used to be obtained according to data analysis unit
Result generation control instruction, control nitrogen feedway, air feeder or the work of mixed gas discharger.
Alternatively, the input equipment includes parametric image acquisition device, and the parametric image acquisition device is used to obtain
Include the image of circulation intermittent hypoxia control data, animal eupnea oxygen concentration threshold value and/or vital signs threshold values, the control
Device processed is additionally operable to processing parameter image, identifies that circulation intermittent hypoxia control data, animal are normally exhaled from the parametric image
Concentration of oxygen inhalation threshold value and/or vital signs threshold values.
Alternatively, the controller also includes graphics processing unit, described image processing unit include image denoising module,
Greyscale transformation module, histogram equalization module and characteristic extracting module, described image denoising module are used to remove parametric image
Noise；The greyscale transformation module is used for the gray value of each pixel in adjusting parameter image；The histogram equalization mould
Block is used to increase the local contrast in parametric image；The feature letter that the characteristic extracting module is used in extracting parameter image
Breath.
Second aspect, the present invention provide the control method of chronic intermittent hypoxia experimental animal system, specifically include following step
Suddenly：
Obtain control data, animal eupnea oxygen concentration threshold value and the vital signs threshold values of circulation intermittent hypoxia；
According to control data control nitrogen feedway, air feeder or the mixed gas of the circulation intermittent hypoxia
Discharger works；
Obtain the vital sign data of the gas content and animal in the rearing-box of acquisition module collection；
By the gas content in the rearing-box and the vital sign data of animal and animal eupnea oxygen concentration threshold value
It is compared with vital signs threshold values, control nitrogen feedway, air feeder or gaseous mixture is controlled according to comparative result
Body discharger.
Alternatively, in addition to control warning device alert.
Beneficial effects of the present invention：
The present invention chronic intermittent hypoxia experimental animal system, using in intermittent hypoxia control data is circulated according to " just
Normal breathing environment-anaerobic respiration environment-eupnea environment " designs, and makes experimental animal according to normal work and rest regularity living
In the outside Chronic Intermittent anaerobic environment of construction, carry out the discontinuity in analogue body caused by apnea recurrent exerbation and lack
Oxygen；Genius morbi is more conformed to using the design method of circulation hypoxemia.Animal is monitored in real time by acquisition module whether in scarce
Oxygen environment and the vital sign in anaerobic environment, it is found that animal life sign interrupts default circulation intermittent hypoxia in time extremely
The programme-control of control data, is passed through air into rearing-box in time so that the oxygen concentration in rearing-box is adapted to animal normally to exhale
Inhale, and greatly reduce the unusual death rate of experiment mice, save experiment fees, increase the practicality and science of system.
The present invention chronic intermittent hypoxia experimental animal system control method, with the rearing-box oxygen concentration gathered in real time,
The vital sign data of animal is compared with threshold value set in advance, the threshold value that sets if more than, then controller interrupts
Circulation intermittent hypoxia control program set in advance, control air feeder cause oxygen concentration in rearing-box to air is passed through
It is adapted to animal breath, genius morbi is more conformed to using the design method of circulation hypoxemia, the reality for having more system control method
With property and science.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art.In all of the figs, similar element
Or part is typically identified by similar reference.In accompanying drawing, each element or part might not be drawn according to the ratio of reality.
Fig. 1 shows the theory diagram of chronic intermittent hypoxia experimental animal system first embodiment provided by the invention；
Fig. 2 shows the theory diagram of another embodiment of chronic intermittent hypoxia experimental animal system provided by the invention；
Fig. 3 shows the first embodiment stream of the control method of chronic intermittent hypoxia experimental animal system provided by the invention
Cheng Tu.
Embodiment
The embodiment of technical solution of the present invention is described in detail below in conjunction with accompanying drawing.Following examples are only used for
Clearly illustrate technical scheme, therefore be intended only as example, and the protection of the present invention can not be limited with this
Scope.
It should be noted that unless otherwise indicated, technical term or scientific terminology used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.
Fig. 1 shows a kind of chronic intermittent hypoxia experimental animal system first embodiment provided by the invention, including input
Equipment 1, nitrogen feedway 2, air feeder 3, acquisition module 4, controller 5 and mixed gas discharger 6, it is described defeated
Enter equipment 1 be used for input circulate intermittent hypoxia control data, animal eupnea oxygen concentration threshold value and vital signs threshold values；Institute
Nitrogen feedway 2 is stated to be used to supply nitrogen according to circulation intermittent hypoxia control data supply nitrogen and according to controller control instruction
Gas；The air feeder 3 is used for according to circulation intermittent hypoxia control data supply air and according to controller control instruction
Supply air；The acquisition module 4 is used for the vital sign data for gathering gas content and animal in rearing-box；The control
Device 5 is used for processing data；According to circulation intermittent hypoxia control data control nitrogen feedway and air feeder supply；Root
Sky is controlled more afterwards according to the data of acquisition module collection and the animal eupnea oxygen concentration threshold value and vital signs threshold values of input
Air feed system and nitrogen feedway supply, the mixed gas discharger 6 are used to exclude the mixed gas in rearing-box.
Circulate intermittent hypoxia control data include circulation intermittent hypoxia gas with various service time, gas with various supply the cycle and
Oxygen concentration in rearing-box.Nitrogen feedway 2, air feeder 3 and mixed gas discharger 6 include breather pipe,
The break-make and flow velocity of solenoid valve control gas are provided with breather pipe.
Air flue repeatedly when we can not simulate OSAS patient sleeps in scientific research
Periodicity chronic intermittent hypoxia caused by obstruction, the long-term unsmooth breath of experimental animal can be caused such as blocking experiment animal respiratory and
Cause Constant hypoxia rather than intermittent hypoxia environment in animal body；Or reach anoxic purpose with pharmaceutical intervention animal, but reality can be made
Test the anaerobic environment that animal is under stress situation, and simultaneously Non-obstructive sleep apnea syndrome patient natural sleep condition
Under intermittent hypoxia environment, therefore in the present invention using in intermittent hypoxia control data is circulated according to " eupnea environment-
Anaerobic respiration environment-eupnea environment " designs, and makes experimental animal according to normal work and rest regularity living in the outside of construction
Chronic Intermittent anaerobic environment, carry out the intermittent hypoxia caused by apnea recurrent exerbation in analogue body；Using circulation
The design method of hypoxemia more conforms to genius morbi.Animal is monitored in real time by acquisition module whether in anaerobic environment and scarce
Vital sign in oxygen environment, it is found that animal life sign interrupts the journey of default circulation intermittent hypoxia control data in time extremely
Sequence controls, and is passed through air into rearing-box in time so that the oxygen concentration in rearing-box is adapted to animal eupnea, and subtracts significantly
The unusual death rate of few experiment mice, saves experiment fees, increases the practicality and science of system.
As the further improvement of above-mentioned technical proposal, acquisition module 4 includes oxygen concentration sensor 41, and the oxygen is dense
Degree sensor 41 is used to gather the oxygen content in rearing-box.Acquisition module also includes electrocardiogram acquisition module 42 and blood oxygen collection mould
Block 43, the electrocardiogram acquisition module 42 are used for the electrocardiogram (ECG) data for gathering animal in rearing-box；The blood oxygen acquisition module 43 is used for
Gather the blood oxygen saturation data of animal in rearing-box.Monitor living environment of the animal in rearing-box in real time by acquisition module
And the vital sign data of animal, oxygen concentration sensor gather the oxygen concentration in rearing-box, the electrocardiogram acquisition module collection heart
Electric transducer, the electrocardiogram (ECG) data of letting animals feed is gathered with EGC sensor, and blood oxygen acquisition module is raised using blood oxygen transducer collection
Support the blood oxygen saturation of animal.Oxygen concentration, electrocardiogram (ECG) data in the rearing-box that controller gathers according to the sensor in real time
Judge whether animal is in anaerobic condition with blood oxygen saturation, if it is judged that animal is not in anaerobic condition, controller after
The continuous circulation intermittent hypoxia control data according to input controls nitrogen feedway, air feeder or mixed gas discharge dress
Put work；If animal is in anaerobic condition, controller then interrupts the circulation according to circulation intermittent hypoxia control program control, control
Mixture in mixed gas discharger processed discharge rearing-box, mixed gas are closed after excluding, control air feeder to
Air is passed through in rearing-box causes the oxygen concentration in rearing-box to be adapted to animal eupnea.
As the further improvement of above-mentioned technical proposal, the system also includes warning device 7, and the controller 5 is also used
Oxygen concentration in the rearing-box gathered according to acquisition module 4 is adopted compared with the oxygen concentration threshold value inputted with acquisition module 4
The animal life sign of collection obtains comparative result compared with the vital signs threshold values inputted, when comparative result exception, report
Alarm device alert.System also includes wireless communication module, and wireless communication module, which is used to send to staff, alarms
Information.Wireless communication module preferably uses cordless communication network, such as mobile phone of GSM, GPRS, 3G, 4G mode to staff
Warning message is sent, unattended, no worker monitor purpose is realized, mitigates the workload of staff, and greatly reduce reality
The unusual death rate of mouse is tested, saves experiment fees, increases the practicality and science of system.
In the present embodiment, experimental animal uses mouse, and the control data for circulating intermittent hypoxia is as follows：Mouse in rearing-box
Oxygen concentration is 21% in eupnea environment, and the oxygen concentration under anaerobic respiration environment in rearing-box is 6%-6.5%, anoxic
Breathing environment is to be passed through nitrogen into eupnea environment, when oxygen concentration reaches 6%-6.5% in rearing-box, hold time for
5-7s, the mixed gas being then discharged out in rearing-box, air is passed through to rearing-box, oxygen concentration is reached 21%, recover normal
Breathing environment.Circulation intermittent hypoxia control data sets in rearing-box that oxygen concentration threshold value is 6%-6.5%, mouse electrocardio threshold value
For 300-800/ minutes, blood oxygen saturation threshold value Wei≤80%.By input equipment input circulation intermittent hypoxia control data and
Vital signs threshold values, controller are normally exhaled according to the oxygen concentration in circulation intermittent hypoxia control data control rearing-box, mouse
Inhale, nitrogen is passed through to rearing-box according to circulation intermittent hypoxia control data, controller is gathered according to oxygen concentration sensor and raised
Current oxygen concentration reaches 6%-6.5% in case, and stopping is passed through nitrogen, 5-7s is maintained, during which, if the vital sign of mouse
Not up to vital signs threshold values, then continued executing with according to circulation intermittent hypoxia control data, if the vital sign of mouse reaches
Vital signs threshold values, then interrupt and perform circulation intermittent hypoxia control data, controller control mixed gas discharger discharge is mixed
Gas is closed, oxygen is passed through to rearing-box, the oxygen concentration in rearing-box is restored to 21%, then carry out next follow
Ring.
As the further improvement of above-mentioned technical proposal, Fig. 2 shows another embodiment of the present invention, the controller 5
Including data pre-processing unit 51, data analysis unit 52 and control instruction generation unit 53, the data pre-processing unit 51
Data for being gathered according to acquisition module are pre-processed；The data analysis unit 52 is used for what is gathered according to acquisition module
Data and the oxygen concentration threshold value and vital signs threshold values of the animal eupnea of input are analyzed, and obtain whether animal is located
In the result of anaerobic environment；The control instruction generation unit 53 is used to generate control according to the obtained result of data analysis unit
System instruction, control nitrogen feedway, air feeder or the work of mixed gas discharger.
Input equipment 1 includes parametric image acquisition device, and the parametric image acquisition device is used to obtain comprising between circulation
The image of breakthrough oxygen control data, animal eupnea oxygen concentration threshold value and/or vital signs threshold values, the controller are additionally operable to
Processing parameter image, circulation intermittent hypoxia control data, animal eupnea oxygen concentration threshold are identified from the parametric image
Value and/or vital signs threshold values.Parametric image acquisition device can use camera or video camera, to containing circulation intermittent hypoxia control
The medium of data processed, animal eupnea oxygen concentration threshold value and/or vital signs threshold values carries out the image that gets parms of taking pictures.Medium
Can be paper or plastics of printing etc..Input equipment uses parametric image acquisition device, can save and be manually entered, especially exist
Need typing largely to circulate intermittent hypoxia control data very convenient, save manpower, and manual entry mistake can be prevented, carry
The accuracy rate of height input.
As the further improvement of above-mentioned technical proposal, the controller also includes graphics processing unit, at described image
Reason unit 54 includes image denoising module 541, greyscale transformation module 542, histogram equalization module 543 and characteristic extracting module
544, described image denoising module 541 is used for the noise for removing parametric image；The greyscale transformation module 542 is used for adjusting parameter
The gray value of each pixel in image；The histogram equalization module 543 is used to increase the local contrast in parametric image；
The characteristic information that the characteristic extracting module 544 is used in extracting parameter image.In order to improve the circulation interruption obtained in image
Anoxic control data, animal eupnea oxygen concentration threshold value, the accuracy of vital signs threshold values, controller include image procossing list
Member, the image denoising module in graphics processing unit remove the noise in parametric image, greyscale transformation module adjusting parameter image
In each pixel gray value, the characteristic information in characteristic extracting module extracting parameter image, the increase of histogram equalization module
Local contrast in parametric image, is easy to feature extraction, the characteristic information in characteristic extracting module extracting parameter image, feature
Information includes the data of service time, gas with various supply cycle and the oxygen concentration in rearing-box.
Second aspect, Fig. 3 show the control method first of chronic intermittent hypoxia experimental animal system provided by the invention
Embodiment, specifically include following steps：
S1：Controller obtains control data, animal eupnea oxygen concentration threshold value and the vital sign of circulation intermittent hypoxia
Threshold value.
S2：Controller controls nitrogen feedway, air feeder according to the control data of the circulation intermittent hypoxia
Or mixed gas discharger work.
S3：Controller obtains the vital sign data of the gas content and animal in the rearing-box of acquisition module collection.
S4：Controller is by the gas content in the rearing-box and the vital sign data of animal and animal eupnea oxygen
Concentration threshold and vital signs threshold values are compared, and control nitrogen feedway, air feeder are controlled according to comparative result
Or mixed gas discharger.
S5：Controller controls warning device alert.
Control data, animal eupnea oxygen concentration threshold value and the vital sign threshold of input equipment input circulation intermittent hypoxia
Value, controller obtain control data, animal eupnea oxygen concentration threshold value and the vital signs threshold values of circulation intermittent hypoxia.Collection
Module monitors the vital sign data of living environment and animal of the animal in rearing-box in real time, and oxygen concentration sensor collection is raised
Support the oxygen concentration in case, electrocardiogram acquisition module collection EGC sensor, with the electrocardio number of EGC sensor collection letting animals feed
According to blood oxygen acquisition module gathers the blood oxygen saturation of letting animals feed using blood oxygen transducer.Controller is real according to the sensor
When the rearing-box that gathers in oxygen concentration, electrocardiogram (ECG) data and blood oxygen saturation judge whether animal is in anaerobic condition, such as
Fruit judges that animal is not in anaerobic condition, and controller continues to supply according to the circulation intermittent hypoxia control data control nitrogen of input
Worked to device, air feeder or mixed gas discharger；If animal is in anaerobic condition, controller, which then interrupts, to be pressed
According to the circulation of circulation intermittent hypoxia control program control, controller control warning device alert, work is notified in time
Personnel, and the mixture in controller control mixed gas discharger discharge rearing-box, mixed gas are closed after excluding, controlled
Air feeder is passed through air into rearing-box and causes the oxygen concentration in rearing-box to be adapted to animal breath, is circulated next time
Control.
The control method of chronic intermittent hypoxia experimental animal system, with the life of the rearing-box oxygen concentration, animal gathered in real time
Sign data is ordered compared with threshold value set in advance, the threshold value set if more than, then controller interruption is preset
Circulation intermittent hypoxia control program, control air feeder causes oxygen concentration in rearing-box to be adapted to animal to air is passed through
Breathing, genius morbi is more conformed to using the design method of circulation hypoxemia, makes practicality and section that system control method has more
The property learned.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme, it all should cover among the claim of the present invention and the scope of specification.
Claims (10)
1. chronic intermittent hypoxia experimental animal system, it is characterised in that including input equipment, nitrogen feedway, air supply
Device, acquisition module, controller and mixed gas discharger, the input equipment are used for the control for inputting circulation intermittent hypoxia
Data, animal eupnea oxygen concentration threshold value and vital signs threshold values；The nitrogen feedway is used for according to breakthrough between circulation
Oxygen control data supplies nitrogen and supplies nitrogen according to controller control instruction；The air feeder is used for according between circulation
Breakthrough oxygen control data supplies air and supplies air according to controller control instruction；The acquisition module is used to gather rearing-box
In gas content and animal vital sign data；The controller is used for processing data；According to circulation intermittent hypoxia control
Data control nitrogen feedway and air feeder supply；The data gathered according to acquisition module and the animal of input are normal
Respiratory oxygen concentration threshold and vital signs threshold values control air feeder and nitrogen feedway to supply more afterwards；The mixing
Gas exhausting device is used to exclude the mixed gas in rearing-box.
2. chronic intermittent hypoxia experimental animal system as claimed in claim 1, it is characterised in that the acquisition module includes oxygen
Gas concentration sensor, the oxygen concentration sensor are used to gather the oxygen content in rearing-box.
3. chronic intermittent hypoxia experimental animal system as claimed in claim 2, it is characterised in that the acquisition module also includes
Electrocardiogram acquisition module and blood oxygen acquisition module, the electrocardiogram acquisition module are used for the electrocardiogram (ECG) data for gathering animal in rearing-box；Institute
State the blood oxygen saturation data that blood oxygen acquisition module is used to gather animal in rearing-box.
4. chronic intermittent hypoxia experimental animal system as claimed in claim 1, it is characterised in that the circulation intermittent hypoxia
The oxygen that control data is included in the service time of the gas with various of circulation intermittent hypoxia, gas with various supply cycle and rearing-box is dense
Degree.
5. chronic intermittent hypoxia experimental animal system as claimed in claim 1, it is characterised in that the system also includes alarm
Device, the oxygen concentration and the oxygen concentration threshold value of input that the controller is additionally operable in the rearing-box according to acquisition module collection are carried out
Compare with acquisition module collection animal life sign with input vital signs threshold values compared with obtain comparative result, when than
During compared with results abnormity, warning device alert.
6. chronic intermittent hypoxia experimental animal system as claimed in claim 5, it is characterised in that the controller includes data
Pretreatment unit, data analysis unit and control instruction generation unit, the data pre-processing unit are used for according to acquisition module
The data of collection are pre-processed；The data analysis unit is being used for the data gathered according to acquisition module and the animal inputted just
The oxygen concentration threshold value and vital signs threshold values often breathed is analyzed, and obtains the result whether animal is in anaerobic environment；
The control instruction generation unit is used to generate control instruction, control nitrogen supply according to the obtained result of data analysis unit
Device, air feeder or the work of mixed gas discharger.
7. the chronic intermittent hypoxia experimental animal system as described in one of claim 1-6, it is characterised in that the input equipment
Including parametric image acquisition device, the parametric image acquisition device is used to obtain comprising circulation intermittent hypoxia control data, moved
The image of thing eupnea oxygen concentration threshold value and/or vital signs threshold values, the controller are additionally operable to processing parameter image, from institute
State and circulation intermittent hypoxia control data, animal eupnea oxygen concentration threshold value and/or vital sign threshold are identified in parametric image
Value.
8. chronic intermittent hypoxia experimental animal system as claimed in claim 7, it is characterised in that the controller also includes figure
As processing unit, described image processing unit includes image denoising module, greyscale transformation module, histogram equalization module and spy
Extraction module is levied, described image denoising module is used for the noise for removing parametric image；The greyscale transformation module, which is used to adjust, joins
The gray value of each pixel in number image；The histogram equalization module is used to increase the local contrast in parametric image；
The characteristic information that the characteristic extracting module is used in extracting parameter image.
9. the control method of chronic intermittent hypoxia experimental animal system as claimed in claim 1, it is characterised in that specifically include
Following steps：
Obtain control data, animal eupnea oxygen concentration threshold value and the vital signs threshold values of circulation intermittent hypoxia；
According to control data control nitrogen feedway, air feeder or the mixed gas discharge of the circulation intermittent hypoxia
Device works；
Obtain the vital sign data of the gas content and animal in the rearing-box of acquisition module collection；
By the gas content in the rearing-box and the vital sign data of animal and animal eupnea oxygen concentration threshold value and life
Life signs threshold values are compared, and control control nitrogen feedway, air feeder or mixed gas to arrange according to comparative result
Go out device.
10. the control method of chronic intermittent hypoxia experimental animal system as claimed in claim 9, it is characterised in that also include
Control warning device alert.
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