CN107684594B - Kidney-tonifying pharmaceutical composition and preparation method and application thereof - Google Patents

Kidney-tonifying pharmaceutical composition and preparation method and application thereof Download PDF

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CN107684594B
CN107684594B CN201610642954.1A CN201610642954A CN107684594B CN 107684594 B CN107684594 B CN 107684594B CN 201610642954 A CN201610642954 A CN 201610642954A CN 107684594 B CN107684594 B CN 107684594B
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tonifying
pharmaceutical composition
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萧伟
李璐
张小强
吴云
孙永城
丁岗
王振中
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JIANGSU KANION YANGGUANG PHARMACEUTICAL Co Ltd
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Jiangsu Kanion Sunshine Pharmaceutical Co ltd
Jiangsu Kanion Pharmaceutical Co Ltd
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Abstract

The invention discloses a kidney-tonifying pharmaceutical composition, a preparation method and application thereof, wherein the composition comprises epimedium, achyranthes root, rhodiola rosea and Chinese yam.

Description

Kidney-tonifying pharmaceutical composition and preparation method and application thereof
Technical Field
The invention belongs to the field of pharmacy, and relates to a kidney-tonifying pharmaceutical composition, and a preparation method and application thereof.
Background
The kidney is the "congenital root and acquired root", and whether the kidney functions normally plays a vital role in the life quality of the human body in the whole process of growth, development, flourishing and aging of the human body. The modern people have great living and mental stress, and the deficiency of the kidney becomes a common disease, and modern science shows that the deficiency of the kidney is accompanied by disorder of a neuroendocrine system, decline of immunity, aging and the like, and seriously threatens the life of people. At present, the kidney tonifying western medicines are numerous, but the symptoms are not treated, and the kidney tonifying effect is not obvious.
The traditional Chinese medicine considers that the kidney deficiency is mostly a long-term accumulation disease, and the kidney deficiency can not be supplemented by a medicine for quick achievement and large supplementation or a kidney-tonifying and yang-strengthening medicine with unknown components.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition for treating kidney deficiency in order to overcome the defects in the prior art.
The purpose of the invention can be realized by the following technical scheme:
a kidney-tonifying pharmaceutical composition is prepared from the following raw materials in percentage by mass: 3 to 80 percent of epimedium, 3 to 70 percent of achyranthes root, 3 to 70 percent of rhodiola root and 3 to 70 percent of Chinese yam, wherein the sum of the mass percentage of the raw materials is 100 percent.
The kidney tonifying pharmaceutical composition is preferably prepared from the following raw materials in percentage by mass: 10-60% of epimedium, 10-50% of achyranthes root, 10-50% of rhodiola root and 10-50% of Chinese yam, wherein the sum of the mass percentages of the raw materials is 100%.
The kidney-tonifying pharmaceutical composition is preferably prepared from the following raw materials, by mass, 40% of epimedium, 25% of achyranthes bidentata, 20% of rhodiola rosea and 15% of Chinese yam.
The kidney tonifying pharmaceutical composition is preferably prepared by the steps of crushing and sieving the prescription amount of raw materials, adding a solvent, extracting, concentrating an extract, drying and crushing.
The preparation method of the kidney tonifying pharmaceutical composition comprises the following steps:
(1) crushing and sieving the raw materials of the traditional Chinese medicine composition, adding an extraction solvent, and extracting for 1-3 times at the temperature of 40-100 ℃ for 0.5-3 hours each time to obtain an extracting solution; wherein the ratio of the mass of the raw materials of the traditional Chinese medicine composition to the volume of the extraction solvent is 1 g: 8-12 mL;
(2) mixing the extractive solutions, filtering, concentrating, spray drying, and vacuum drying or lyophilizing under reduced pressure to obtain dry extract;
(3) and crushing the obtained dry extract, and sieving the crushed dry extract by a sieve of 80-100 meshes to obtain the kidney tonifying medicinal composition.
Wherein, the extraction solvent in the step (1) is preferably water or an organic solvent, and the organic solvent is further preferably one or more of petroleum ether, ethyl acetate, chloroform or ethanol; the extraction method in the step (1) is preferably microwave extraction, reflux extraction or supercritical extraction.
The application of the kidney-tonifying medicinal composition in preparing a medicament for treating kidney deficiency is provided.
A medicine for treating kidney deficiency comprises the traditional Chinese medicine composition.
The medicament is preferably any clinically-allowable dosage form prepared by mixing the traditional Chinese medicine composition with pharmaceutically-acceptable auxiliary materials.
The dosage form is preferably selected from decoction, granules, capsules, tablets, mixture, pills or powder.
Has the advantages that:
according to records in Chinese pharmacopoeia, epimedium has the functions of tonifying kidney yang, strengthening muscles and bones and dispelling wind-damp, and a plurality of medicines for treating kidney deficiency contain the relevant components of epimedium; research shows that the rhodiola rosea can improve the SOD activity in vivo and reduce the content of triglyceride by improving the immunity and the muscle movement capability of organisms, thereby playing the role of anti-aging; achyranthes bidentata enters liver and kidney meridians, has the effects of tonifying liver and kidney, strengthening bones and muscles, activating blood and stimulating menstrual flow, inducing diuresis and treating stranguria, and is often used as a traditional medicine for strengthening bones and muscles, activating blood and dissolving stasis; the rhizoma Dioscoreae can improve kidney deficiency by regulating immunity, resisting oxidation, and treating kidney injury. The combination of the 4 medicines can play roles of strengthening bones and muscles, tonifying liver and kidney, nourishing yin and tonifying yang from different ways, and the kidney tonifying effect of the composition is obviously superior to the combination of three or two medicines.
In the pharmacological tests described below, the influence of the kidney-tonifying pharmaceutical composition on kidney deficiency model mice is mainly examined.
1. Preparation of Kidney tonifying pharmaceutical composition sample
Adding 80g of crushed and sieved herba epimedii, 50g of radix achyranthis bidentatae, 40g of rhodiola rosea and 30g of Chinese yam into 2L of water for 3 times, extracting for 1 hour each time, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.10-1.13(60 ℃); vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve.
The obtained dry paste powder is prepared into the following concentration by distilled water respectively: 0.5g crude drug/mL, 0.25g crude drug/mL, and 0.125g crude drug/mL in high, medium, and low dosage groups.
2. Preparation of samples of related prescription pharmaceutical compositions
2.1 the prescription of the barrenwort medicine:
adding 50g of the crushed and sieved achyranthes roots, 40g of rhodiola rosea and 30g of Chinese yam into 1.2L of water for 3 times, extracting for 1 hour each time, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.10-1.13(60 ℃); vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve. And preparing the obtained dry extract powder into a solution with the concentration of 0.5g crude drug/mL by using distilled water.
2.2 the prescription of the radix achyranthis bidentatae lacking medicine is as follows:
adding 80g of crushed and sieved herba epimedii, 40g of rhodiola rosea and 30g of Chinese yam into 1.5L of water for 3 times, extracting for 1 hour each time, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.10-1.13(60 ℃); vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve. And preparing the obtained dry extract powder into a solution with the concentration of 0.5g crude drug/mL by using distilled water.
2.3 the prescription of the rhodiola root-lacking medicament:
adding 80g of crushed and sieved herba epimedii, 50g of radix achyranthis bidentatae and 30g of Chinese yam into 1.6L of water for 3 times, extracting for 1 hour each time, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.10-1.13(60 ℃); vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve. And preparing the obtained dry extract powder into a solution with the concentration of 0.5g crude drug/mL by using distilled water.
2.4 the Chinese yam medicine formula:
adding 80g of crushed and sieved herba Epimedii, 50g of radix Achyranthis bidentatae and 40g of radix Rhodiolae into 1.7L of water, extracting for 3 times (each time for 1 hour), filtering, combining filtrates, and concentrating under reduced pressure to obtain extract with relative density of 1.10-1.13(60 deg.C); vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve. And preparing the obtained dry extract powder into a solution with the concentration of 0.5g crude drug/mL by using distilled water.
2.5 the medicine formula of the barrenwort and the twotooth achyranthes root is as follows:
adding 40g of crushed and sieved rhodiola rosea and 30g of yam into 0.7L of water for 3 times, extracting for 1 hour each time, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.10-1.13(60 ℃); vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve. And preparing the obtained dry extract powder into a solution with the concentration of 0.5g crude drug/mL by using distilled water.
3. Grouping and administration of drugs
Experimental mice were randomly divided into 11 groups of 16 mice each, each half male and female. Mice were gavaged once daily with a dosing volume of 0.3mL/10 g. The dosages are respectively as follows: (1) feeding distilled water to normal control group; (2) model group: 5mg/10g of hydroxyurea; (3) positive control group: 5mg/10g of hydroxyurea and 40mg/10g of Liuwei Dihuang pills; (4) high dose group of kidney tonifying pharmaceutical composition: 5mg/10g of hydroxyurea + 15g of crude drug/kg of sample; (5) the dosage group of the kidney tonifying pharmaceutical composition comprises: 5mg/10g of hydroxyurea + 7.5g of crude drug/kg of sample; (6) the low dose group of the kidney tonifying pharmaceutical composition comprises: 5mg/10g of hydroxyurea + 3.75g of crude drug/kg of sample, (7-11) related prescription pharmaceutical composition dose groups: 5mg/10g of hydroxyurea and 15g of crude drug/kg of related samples; the administration was continued for 9 d. Weigh and record body weight every 3 days. And (3) observing the autonomous activity of the mouse by using an autonomous activity instrument 30min after the last administration, and recording the activity frequency of the mouse within 5 min. All animals were then sacrificed. The adrenal gland, the testis, the prostate gland, the seminal vesicle gland, the uterus, the ovary and the like of the mouse are picked up and weighed by a precise electronic balance. Statistical treatment was performed and the significance of differences between groups was examined using the t-test.
4. Results of the experiment
4.1 Effect on model mouse body weight
TABLE 1 Effect of Kidney tonifying pharmaceutical composition on body weight of mice with renal deficiency: (
Figure BDA0001070973850000041
n＝16)
Figure BDA0001070973850000042
Note: in comparison to the blank set, the data is,#P<0.05，##P<0.01; in comparison to the set of models,*P<0.05，**P<0.01。
the experimental result shows that the weight of the mice in the model group is obviously reduced (P <0.01) compared with that in the blank group after the hydroxyurea is administered, and compared with the model group, the weight of the mice in the high-dose, medium-dose and low-dose groups of the composition is obviously reduced less than that in the model group (P <0.01) on the 3 rd day and the 9 th day after the experiment, which indicates that the kidney-tonifying medicinal composition has certain improvement effect on the weight reduction of the mice in the hydroxyurea kidney deficiency model, and the effect is obviously better than that of the positive medicament.
In the prescription-splitting research, partial experimental groups can improve the weight reduction (P <0.05) of mice without one prescription, but the effect is not better than that of each dosage group (P <0.01) of the prescription, and the dosages of all prescription dose groups are the same as those of the high-dosage group of the prescription, so that the prescription has the following another advantage: the dosage of the medicine can be reduced by adding one medicine; the traditional Chinese medicine composition has no two medicinal flavors of epimedium and achyranthes root, and has no obvious treatment effect on weight loss of a kidney deficiency model.
4.2 Effect on autonomous Activity in Kidney deficiency model mice
TABLE 2 influence of Kidney tonifying pharmaceutical composition on the number of spontaneous renal deficiency mice Activity
Figure BDA0001070973850000051
n＝16)
Figure BDA0001070973850000052
Note: in comparison to the blank set, the data is,#P<0.05，##P<0.01; in comparison to the set of models,*P<0.05，**P<0.01; compared with the group with high dosage of the prescription,ΔP<0.05，ΔΔP<0.01。
the results show that the number of self-activity times of the animals in the model group is obviously less than that of the animals in the normal control group and the animals in the high, medium and low dose groups of the kidney-tonifying medicinal composition (P is less than 0.01). The kidney tonifying medicinal composition has an effect of increasing the autonomous activity of a kidney deficiency model mouse caused by hydroxyurea kidney.
In the experiment of the formula splitting group, the lack of the Chinese herbal medicine formula can increase the times of autonomous activities (P is less than 0.05) of animals in the model group, but the effect is obviously different from that of each dosage group of the formula; the mountain-lacking medicine group has obvious difference (P is less than 0.01) compared with the high-dose group of the prescription when the doses are the same; the rest of the medicinal herbs and the rest of the medicinal herbs lack two medicinal herbs have no obvious effect on increasing the times of autonomous activities of animals in a model group.
4.3 Effect on development of additional organs in mice model of renal deficiency
TABLE 3 Effect of Kidney tonifying pharmaceutical composition on the development of renal deficiency mouse appendage organ: (
Figure BDA0001070973850000053
n＝8)
Figure BDA0001070973850000054
Figure BDA0001070973850000061
Note: in comparison to the blank set, the data is,#P<0.05，##P<0.01; in comparison to the set of models,*P<0.05，**P<0.01, compared with the high-dose group of the prescription,ΔP<0.05，ΔΔP<0.01。
the results show that after the hydroxyurea is administered, the weight coefficients of testis, seminal vesicle gland, prostate, uterus and ovary of the model group mice are all obviously lower than that of the normal control group (P < 0.01). Compared with the model group, the weight coefficients of testis, seminal vesicle gland, prostate, uterus and ovary of mice in the high, medium and low dosage group of the kidney-tonifying medicinal composition are obviously higher than those of the model group (P is less than 0.05, and P is less than 0.01). The kidney-tonifying medicinal composition has certain improvement effect on the atrophy and weight reduction of the sex organs of the animal model mouse with the kidney deficiency syndrome.
After the prescription is removed, the weight coefficient of the seminal vesicle gland and the prostate gland of part of single medicine prescription removal groups is obviously higher than that of a model group (P is less than 0.05), but the weight coefficient of the ovary is not as high as that of each dosage group of the prescription, and the weight coefficient of the ovary has significant difference (P is less than 0.05, and P is less than 0.01) compared with that of a high dosage group of the prescription under the same dosage condition; the uterus weight coefficient of part of single medicine prescription-dismantling groups is obviously higher than that of a model group (P <0.05), but is only equivalent to that of a low-dose group, but is inferior to that of middle-dose and high-dose groups, and has significant difference (P <0.05) compared with that of a high-dose group under the condition of equivalent dose; the influence of each prescription formula on the development of the testis is not obvious; the prescription group without two medicines has no obvious effect on the atrophy and the weight of the sex organs of the mice of the animal model with the kidney deficiency syndrome.
4.4 Effect on adrenal weight coefficient of Kidney deficiency model mouse
TABLE 4 Effect of Kidney tonifying pharmaceutical composition on weight of adrenal gland of kidney deficiency mice: (
Figure BDA0001070973850000062
n＝16)
Figure BDA0001070973850000063
Figure BDA0001070973850000071
Note: in comparison to the blank set, the data is,#P<0.05，##P<0.01; in comparison to the set of models,*P<0.05，**P<0.01。
the results show that the adrenal weight coefficient of the model group animals is significantly lower than that of the normal control group (P < 0.01). Compared with the model group, the adrenal weight coefficients of mice with high, medium and low doses of the kidney-tonifying medicinal composition are also obviously higher than that of the model group (P is less than 0.01), which shows that the kidney-tonifying medicinal composition and related medicaments have certain improvement effect on the reduction of the adrenal weight of the mice with the animal model of kidney deficiency. The effect of the medicine combination of the prescription removing group on the weight of adrenal gland is not obvious, and the effect is not as good as that of the medicine combination of the whole formula.
5. Conclusion
The pharmaceutical composition can obviously increase the times of autonomous activities of mice with the syndrome of kidney deficiency of hydroxyurea, inhibit the weight loss degree of model animals with the kidney deficiency caused by hydroxyurea, and obviously improve the sexual organs and the weight atrophy of adrenal glands of the model animals with the kidney deficiency, and the formula of 4 medicines is obviously superior to the functions of 3 medicines and 2 medicines, and the 4 medicines have good protection effect on the kidney and can be developed into health-care products or medicines for treating the kidney deficiency.
Detailed Description
The following further describes particular embodiments of the present invention to facilitate further understanding of the present invention by those skilled in the art, and does not constitute a limitation to the right thereof.
Example 1: preparation of kidney-tonifying medicinal composition
Adding 2.0L of water into 160g of epimedium herb, 20g of achyranthes root, 14g of rhodiola rosea and 6g of Chinese yam which are crushed and sieved by a 20-mesh sieve, decocting and extracting for 2 times, wherein each time is 1.5 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.11; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 90 mesh sieve to obtain the final product.
Example 2: preparation of kidney-tonifying medicinal composition
Adding 20g of herba epimedii, 140g of radix achyranthis bidentatae, 20g of rhodiola rosea and 20g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2.0L of water, decocting and extracting for 2 times, wherein each time lasts for 1.5 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.11; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 90 mesh sieve to obtain the final product.
Example 3: preparation of kidney-tonifying medicinal composition
Adding 80g of herba epimedii, 60g of radix achyranthis bidentatae, 6g of rhodiola rosea and 54g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2.0L of water, decocting and extracting for 2 times, wherein each time is 1.5 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.11; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 90 mesh sieve to obtain the final product.
Example 4: preparation of kidney-tonifying medicinal composition
Adding 20g of herba epimedii, 60g of radix achyranthis bidentatae, 100g of rhodiola rosea and 20g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2.0L of water, decocting and extracting for 2 times, wherein each time lasts for 1.5 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.11; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 90 mesh sieve to obtain the final product.
Example 5: preparation of kidney-tonifying medicinal composition
Adding 60g of herba epimedii, 20g of radix achyranthis bidentatae, 20g of rhodiola rosea and 100g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2.0L of water, decocting and extracting for 2 times, wherein each time is 1.5 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.11; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 90 mesh sieve to obtain the final product.
Example 6: preparation of kidney-tonifying medicinal composition
Adding 80g of herba epimedii, 50g of radix achyranthis bidentatae, 40g of rhodiola rosea and 30g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 1.6L of water, decocting and extracting for 1 time, wherein each time is 0.5 hour, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.10; vacuum drying the obtained extract at 40 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain the final product.
Example 7: preparation of kidney-tonifying medicinal composition
Adding 80g of herba epimedii, 50g of radix achyranthis bidentatae, 40g of rhodiola rosea and 30g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2.0L of water, decocting and extracting for 2 times, wherein each time is 1.5 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.11; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 90 mesh sieve to obtain the final product.
Example 8: preparation of kidney-tonifying medicinal composition
Adding 80g of herba epimedii, 50g of radix achyranthis bidentatae, 40g of rhodiola rosea and 30g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2.4L of water, decocting and extracting for 3 times, each time for 2 hours, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.12; vacuum drying the obtained extract at 100 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 100 mesh sieve to obtain the final product.
Example 9: preparation of kidney-tonifying decoction
Adding 80g of herba Epimedii, 50g of Achyranthis radix, 40g of radix Rhodiolae, and 30g of rhizoma Dioscoreae which are pulverized and sieved with 20 mesh sieve into 2L of water, decocting and extracting for 3 times, each time for 1 hour, filtering, and mixing filtrates to obtain the final product.
Example 10: preparation of kidney-tonifying granules
Adding 80g of herba epimedii, 50g of radix achyranthis bidentatae, 40g of rhodiola rosea and 30g of Chinese yam which are crushed and sieved by a 20-mesh sieve into 2L 60% ethanol for extraction for 3 times, each time for 1 hour, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.1; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain the final product. Mixing the kidney invigorating medicinal composition and dextrin (5:1, g/g), wet granulating with 95% ethanol as binder, drying, and grading.
Example 11: preparation of kidney tonifying tablet
Adding 80g of crushed and sieved epimedium herb, 50g of achyranthes root, 40g of rhodiola rosea and 30g of Chinese yam into 2L 60% ethanol for extraction for 3 times, each time for 1 hour, filtering, combining filtrates, and concentrating under reduced pressure to obtain an extract with the relative density of 1.1; vacuum drying the obtained extract at 70 deg.C under reduced pressure to obtain dry extract, pulverizing, and sieving with 80 mesh sieve to obtain the final product. Mixing the kidney invigorating medicinal composition and dextrin (5:1, g/g), wet granulating with 95% ethanol as binder, drying, grading, adding 1% magnesium stearate, mixing, tabletting, coating film coat, and packaging.

Claims (6)

1. The kidney-tonifying pharmaceutical composition is characterized by being prepared from the following raw materials in percentage by mass: epimedium 40%, achyranthes root 25%, rhodiola root 20% and Chinese yam 15%.
2. The kidney-tonifying pharmaceutical composition as claimed in claim 1, wherein the kidney-tonifying pharmaceutical composition is prepared by pulverizing and sieving the prescribed amount of raw materials, adding solvent water, extracting, concentrating the extract, drying, and pulverizing.
3. The method for preparing a kidney-tonifying pharmaceutical composition according to claim 1, comprising the steps of:
(1) crushing and sieving the raw materials of the traditional Chinese medicine composition, adding an extraction solvent water, and extracting for 1-3 times at the temperature of 40-100 ℃ for 0.5-3 hours each time to obtain an extracting solution; wherein the ratio of the mass of the raw materials of the traditional Chinese medicine composition to the volume of the extraction solvent is 1 g: 8-12 mL;
(2) mixing the extractive solutions, filtering, concentrating, spray drying, and vacuum drying or lyophilizing under reduced pressure to obtain dry extract;
(3) and crushing the obtained dry extract, and sieving the crushed dry extract by a sieve of 80-100 meshes to obtain the kidney tonifying medicinal composition.
4. The method according to claim 3, wherein the extraction method in step (1) is selected from the group consisting of microwave extraction and reflux extraction.
5. The use of the kidney-tonifying pharmaceutical composition according to claim 1 in the preparation of a medicament for the treatment of kidney deficiency.
6. A medicament for treating kidney deficiency, which is characterized by comprising a clinically allowable dosage form prepared by mixing the kidney tonifying pharmaceutical composition as claimed in claim 1 and pharmaceutically acceptable auxiliary materials; the dosage form is selected from decoction, granules, capsules, tablets, mixture, pills or powder.
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CN101584758A (en) * 2008-05-24 2009-11-25 刘敏 A kind of pharmaceutical composition for the treatment of kidney deficiency
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CN101099814A (en) * 2007-07-18 2008-01-09 广州中医药大学 Medicinal composition for delaying male senility
CN101129443A (en) * 2007-09-13 2008-02-27 柳忠润 Rhodiola rosea formulated product and method of producing the same
CN101584758A (en) * 2008-05-24 2009-11-25 刘敏 A kind of pharmaceutical composition for the treatment of kidney deficiency
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