CN107684566A - A kind of medicine for treating lung cancer and preparation method thereof - Google Patents
A kind of medicine for treating lung cancer and preparation method thereof Download PDFInfo
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- CN107684566A CN107684566A CN201710956488.9A CN201710956488A CN107684566A CN 107684566 A CN107684566 A CN 107684566A CN 201710956488 A CN201710956488 A CN 201710956488A CN 107684566 A CN107684566 A CN 107684566A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/748—Oldenlandia or Hedyotis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
Abstract
The present invention relates to a kind of process for preparing medicine for treating lung cancer.A, American cockroach crushes, and ethanol soaks to obtain mixed liquor；B, 30~50 DEG C of ultrasonic extractions of mixed liquor, the 30KHz of ultrasonic power 20, obtain ethanol extract；C, ethanol extract is concentrated to give American cockroach polypide ethanol concentrate；D, ethanol concentrate adds 7~10 times of water, and stirring, heating, standing are allowed to be layered；E, lower floor's aqueous, filtering, charcoal absorption obtained by step D；F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, washing active carbon, and water lotion discards；Eluent, filter and must elute filtrate；Elution filtrate obtained by step F is concentrated to give to the American-cockroach-extract medicinal extract for the treatment of lung cancer G,；H, American-cockroach-extract drying, the crushing for the treatment of lung cancer obtained by step G obtain the American-cockroach-extract finished product for treating lung cancer, have significant adjuvant treatment effect to curing lung cancer.
Description
Technical field
The present invention relates to a kind of process for preparing medicine, more particularly to a kind of process for preparing medicine for treating lung cancer.
Background technology
Lung cancer be lung tissue inner cell growth it is out of hand caused by a kind of disease.In the past 50 years, the incidence of disease of lung cancer
Increase year by year with the death rate, current global No.1 cancer killer is developed into from a kind of orphan disease in 20th century.According to system
Meter, in many cities of industrialized country and China, the incidence of disease of lung cancer ranks male's kinds of tumor first and women is common
Malignant tumour second, and as the most common cause of death in malignant tumour.Expect 2025, China, which is often only, to be died from
The number of lung cancer will turn into the first in the world lung cancer big country close to 1,000,000.
According to the differentiation degree and morphological feature of lung carcinoma cell, lung cancer is mainly clinically divided into ED-SCLC
(small cell lung cancer, SCLC) and non-small cell lung cancer (non-small cell lung cancer,
NSCLC) two major class, wherein non-small cell lung cancer account for 85% or so.Death toll caused by NSCLC compares forefront in the world
The summation of gland cancer, breast cancer and colon cancer is more.Because NSCLC patient's early stage is without obvious characteristic, although diagnostic method and treatment
Means have made great progress, and are still in cancer of late stage when 70% NSCLC cancer patients make a definite diagnosis for the first time, wherein about 40%
Patient lose the chance of operative treatment.
Do not have selectivity yet with chemotherapy, it is difficult to effective drug concentration or therapeutic dose are formed in tumor by local, it is existing
Embolic chemotherapy only have certain effect to patient NSCLC less than 50%, almost all of patient can turn in chemotherapy process
It is changed into drug resistance lung cancer, causes failure in treatment.Simple raising chemotherapeutics concentration is limited by general toxic reaction again.
Relative to western medicine, traditional Chinese medicine is same indispensable in treatment of cancer.Chinese medicine can improve patient and work(is immunized
Energy and anti-cancer ability, contribute to patient smoothly to spend convalescence.However, there is slowly effect, curative effect not in simple treatment by Chinese herbs
The inferior position such as definite, the seldom Main Means as treatment of cancer.
American cockroach is Insecta Pterigota Blattaria Blattidae Periplaneta insect, is commonly called as cockroach, is body in Blattidae
The maximum insect of product, originates in South America.American cockroach is traditional Chinese medicine, be used as medicine first recorded in《Sheng Nong's herbal classic》, call " taste
It is salty, tremble with fear.Main blood stasis, disease heavily fortified point, fever and chills, break accumulation, and laryngopharynx is fought, interior cold, s.m.p ", to treat infantile malnutrition, furunculosis, carbuncle swells, larynx
Moth, nameless sores or boils, syphilis and poisonous snake, Wu pines such as are bitten at the disease.Because effective ingredient is many existing for American cockroach, commonly carry
Taking mode, its effect can not reach optimal for specified disease, therefore needs are special to American cockroach progress according to different diseases
Extraction, so that its therapeutic effect to certain disease reaches optimal.
The content of the invention
It is an object of the present invention to provide a kind of medicine for treating lung cancer and preparation method thereof, the U.S. obtained using the preparation method
The big Lian active ingredients in continent have significant adjuvant treatment effect to lung cancer.
The present invention realizes above-mentioned purpose using following preparation method：
A, by dry American cockroach polypide cross 10-40 mesh sieve crush, by gained American cockroach coarse powder concentration be 60%~
95% ethanol immersion, ethanol liquid level is set to be higher by 5~10cm of coarse powder liquid level, closed preservation one is more than week, less than 18 months,
Coarse powder mixed liquor is made；
B, coarse powder mixed liquor obtained by step A is subjected to 3~5 ultrasonic extractions at a temperature of 30~50 DEG C, ultrasonic power is：
20-30KHz, when to coarse powder mixed liquor extract for the first time, the ethanol of 5~8 times of amount concentration 60%~95% of overstriking powder weight, carry
It is 30~60min to take the time；The ethanol of 3~6 times of amount concentration 60%~95% of overstriking powder weight, extraction are extracted after second every time
Time is 2~50min；Merge extract solution, filter to obtain American cockroach polypide ethanol extract；
C, ethanol extract obtained by step B is depressurized under conditions of vacuum -0.05~-0.06MPa, 50~60 DEG C of temperature dense
Contracting, to proportion be 1.05~1.15 when, obtain American cockroach polypide ethanol concentrate；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7~10 times of amounts of its weight, rise while stirring D,
Temperature is to 85~95 DEG C, then constant temperature stirs 10~60 minutes, and mixing speed is 20~60 revs/min, and then insulation stands 1~5 hour
It is allowed to be layered；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
0.5 ~ 1.0 times of amount medicinal carbon of gained ethanol concentrate stirs 30 ~ 60 minutes at a temperature of 40 ~ 60 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first steamed with 10 ~ 30 times of amounts of medicinal carbon
Distilled water is washed, and water lotion discards；Again with the eluent of 30 ~ 60 times of amounts of medicinal carbon, eluent is collected, through filter paper mistake
Filter, must elute filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa,
Be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G 50 DEG C~60 DEG C temperature, vacuum -0.04~-
It is dried in vacuo under conditions of 0.07MPa, crushes as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Preferably, step B is extracted in accordance with the following methods, and coarse powder mixed liquor obtained by step A is carried out into ultrasonic extraction 3 times：
First time ultrasonic extraction：30~35 DEG C, ultrasonic power 20-25KHz of temperature, 5~8 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 30~60min；
Second of ultrasonic extraction：35~40 DEG C, ultrasonic power 23-28KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 20~50min；
Third time ultrasonic extraction：40~50 DEG C, ultrasonic power 25-30KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 35~50min；
It is highly preferred that in step B, first time ultrasonic extraction：30~32 DEG C, ultrasonic power 20-22KHz of temperature, overstriking powder weight 5
The ethanol of~8 times of amount concentration 60%~95%, extraction time is 35~45min.
It is highly preferred that in step B, second of ultrasonic extraction：35~38 DEG C, ultrasonic power 23-25KHz of temperature, overstriking powder
The ethanol of 3~6 times of amount concentration 75%~95% of weight, extraction time is 35~45min.
It is highly preferred that in step B, third time ultrasonic extraction：43~45 DEG C, ultrasonic power 28-30KHz of temperature, overstriking powder
3~6 times of amount concentration 80-95% of weight ethanol, extraction time is 45~50min.
It is highly preferred that step D：American cockroach polypide ethanol concentrate obtained by step C is added into 7~10 times of amounts of its weight
Purified water, warming while stirring is to 85~90 DEG C, then constant temperature stirs 45~60 minutes, and mixing speed is 45~60 revs/min, then
Insulation stands 4~5 hours and is allowed to be layered.
It is highly preferred that in step E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters,
0.5-0.8 times of filtrate of addition is measured medicinal carbon and stirred 45 minutes at a temperature of 50 DEG C in gained filtrate, it is fully adsorbed；
It is highly preferred that in step F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, decoction is released, first with medicinal work
Property 20 times of charcoal amount distillation water washings, water lotion discards；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected,
Filtered through filter paper, filtrate must be eluted, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to treatment lung cancer, specific compatibility and preparation method are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, is formulated by following raw material：Oldenlandia diffusa 40-50 parts, the red sage root
The American-cockroach-extract medicinal extract 0.15-0.25 parts for the treatment lung cancer that 10-20 parts, curcuma zedoary 8-12 parts, step G are obtained or equivalent
The drying fine powder that medicinal extract is produced to obtain by step H.
Preferentially, a kind of medicine for treating lung cancer, is counted in parts by weight, is formulated by following raw material：Oldenlandia diffusa
42-48 parts, the American-cockroach-extract medicinal extract 0.18- for the treatment lung cancer that red sage root 12-18 parts, curcuma zedoary 9-11 parts, step G are obtained
The drying fine powder that 0.22 part or equivalent medicinal extract are produced to obtain by step H.
The preparation method for treating the medicine of lung cancer is as follows：By oldenlandia diffusa 40-50 parts, red sage root 10-20 parts, curcuma zedoary 8-12
Part feeds intake in proportion, after conventional decoct soup water extraction or the extraction of second alcohol extraction process, is proportionally added into American-cockroach-extract medicinal extract
It is well mixed.
The decoct soup water extraction decoction being prepared using the preparation method can directly be taken as decoction, or decoction is condensed into
Medicinal extract or the direct warm water of liquid extract are taken after mixing it with water, or add suitable auxiliary material and the preparations such as tablet, capsule, pill, granular-grade, mixture are made
Preserve and take to facilitate.
Beneficial effect：
1st, insoluble matter in the obtained American-cockroach-extract medicinal extract water of auxiliary treatment lung cancer is extracted using income approach of the present invention
Amount is less than 3%, and total amino acid content is higher than 60.0%, is higher than 45% with reference to amino acid content.
2nd, there is the American cockroach active ingredient being prepared using the method for the invention preferably auxiliary to control to lung cancer
Therapeutic effect, the medicine can be used for auxiliary treatment after operation of lung cancer, play lifting body immunity, repair damaged lung cell, promote
Enter lung cells regeneration, accelerate lung's non-viable non-apoptotic cell and come off, recover after accelerating pulmonary surgery, reduce pulmonary surgery excision and control
Recurrence rate after treatment.
3rd, for the lung cancer patient for receiving radiotherapy, embolic chemotherapy is treated, this medicine is taken, human immunological competence can be strengthened,
Reduce radiotherapy, the injury of chemotherapy on human body, promote lung cells regeneration, accelerate lung's non-viable non-apoptotic cell and come off, it is quick to repair
Damaged lung cell, reduces lung tumors, the extending life time.
4th, technique of the present invention extraction production temperature is relatively low, can effectively protect to the effective American cockroach of lung cancer into
Part, lift lung cancer cure rate.
Specific embodiment
Embodiment 1
The process for preparing medicine of extraction treatment lung cancer, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 10 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 60%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 5cm, closed to preserve 18 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to 3 ultrasonic extractions at a temperature of 30 DEG C, ultrasonic power is：20KHz is right
When coarse powder mixed liquor extract for the first time, the ethanol of 5 times of amount concentration 60% of overstriking powder weight, extraction time 30min；Second
The ethanol of 3 times of amount concentration 60% of overstriking powder weight, extraction time 2min are extracted after secondary every time；Merge extract solution, filter beautiful
The big Lian polypides ethanol extract in continent；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.05MPa, temperature 50 C, is to proportion
When 1.05, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 10 minutes, mixing speed is 20 revs/min, and then insulation stands and is allowed to be layered for 1 hour；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
0.5 times of amount medicinal carbon of gained ethanol concentrate stirs 30 minutes at a temperature of 40 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 10 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 30 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of temperature 50 C, vacuum -0.05MPa, being concentrated into proportion is
When 1.20, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G in 50 DEG C of DEG C of temperature, vacuum -0.04MPa condition
Lower vacuum drying, crush as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment lung cancer for extracting to obtain using income approach of the present invention
1.2%, total amino acid content 69.3%, with reference to amino acid content 53.7%.
Case：
30 cases of lung cancer, man 15, female 15 are age 31-74 year, average（45±3.5）Year, the course of disease are average at -6 years 10 months
The course of disease：42 ± 5.5 months.
Auxiliary curative effect standard（1）It is effective：After 1st month terminates, wound recovers preferable after corrective surgery, and respiratory function recovers
Normally, body immune ability is restored, the non-further growth of residual tumor cells, transfer；After 3rd month terminates, Huan Zheshen
Body immunocompetence gets a promotion, the non-further growth of residual tumor cells, transfer；After 6th month terminates, patient body immunity energy
Power recovers normal, and residual tumor cells have been reduced；After 12nd month terminates, patient body immunocompetence is normal, tumors remaining
Cell is further reduced, and Lung Cancer Recurrence rate is less than 40%；（3）Effectively：2nd month terminate after, after corrective surgery wound recover compared with
Good, respiratory function recovers normal, and body immune ability is restored, the non-further growth of residual tumor cells, transfer；5th
After the moon terminates, patient body immunocompetence gets a promotion, the non-further growth of residual tumor cells, transfer；Terminate within 8th month
Afterwards, patient body immunocompetence recovers normal, and residual tumor cells have been reduced；After 12nd month terminates, patient body is immunized
Ability is normal, and residual tumor cells are further reduced, and Lung Cancer Recurrence rate is less than 45%；（3）It is invalid：Cardinal symptom, sign are without bright
It is aobvious to improve.
Auxiliary treating method one：Patient received Lung Cancer Cases After Resection operation before the treatment, and Post operation is oral the present embodiment step
The American-cockroach-extract medicinal extract for the treatment of lung cancer prepared by rapid G, takes American-cockroach-extract medicinal extract 0.2g to be added to 30ml temperature
Stirred in water, be one day dosage.Every natural gift morning, noon, evening take three times, each taking 10ml, even served 12 months.
Auxiliary treating method two：Patient received Lung Cancer Cases After Resection operation before the treatment, and Post operation is oral the present embodiment step
The American-cockroach-extract finished product fine powder of auxiliary treatment stomach cancer prepared by rapid H, takes American-cockroach-extract medicinal extract obtained by step G
The dried fine powders of 0.2g, which are added in 30ml warm water, to stir, and is one day dosage.Every natural gift morning, noon, evening take three times, often
It is secondary to take 10ml, it even served 12 months.
The result of auxiliary treating method one：Effective 18 for the treatment of group, effective 9, invalid 3, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The result of auxiliary treating method two：Effective 18 for the treatment of group, effective 9, invalid 3, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to auxiliary treatment lung cancer, specific compatibility, preparation, instructions of taking are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, and its compatibility of drugs and preparation method are as follows：By oldenlandia diffusa 45g,
Red sage root 15g, curcuma zedoary 10g feed intake in proportion, add cold water soak 30 minutes, and water did not had medicinal material 2-5cm, have soaked after intense fire boils,
Turn slow fire infusion 20 minutes, filter out the dregs of a decoction, decoct 2 times again, the treatment lung cancer use that step G is obtained is added after merging 3 concoctions
American-cockroach-extract medicinal extract 0.2g or the equivalent medicinal extract drying fine powder that produces to obtain by step H, be well mixed.This medicine
Dosage is one day dosage, and every natural gift morning, noon, evening take three times.
With Chinese medicine compatibility auxiliary treatment lung cancer result：
Auxiliary treating method three：Patient received Lung Cancer Cases After Resection operation before the treatment.After Chinese medicine extraction plus what step G was obtained controls
The American-cockroach-extract medicinal extract 0.2g for the treatment of lung cancer, effective 19 for the treatment of group, effective 10, invalid 1, in therapeutic process,
Patient has no adverse reaction, is paid a return visit after half a year without recurrence.
Auxiliary treating method four：Patient received Lung Cancer Cases After Resection operation before the treatment.Add 0.2g medicinal extract after Chinese medicine extraction
The drying fine powder for producing to obtain by step H：Effective 19 for the treatment of group, effective 10, invalid 1, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
Embodiment 2
The process for preparing medicine of extraction treatment lung cancer, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 40 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 95%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 10cm, closed to preserve 7 days, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to 5 ultrasonic extractions at a temperature of 50 DEG C, ultrasonic power is：30KHz is right
When coarse powder mixed liquor extract for the first time, the ethanol of 8 times of amount concentration 95% of overstriking powder weight, extraction time 60min；Second
The ethanol of 6 times of amount concentration 95% of overstriking powder weight, extraction time 50min are extracted after secondary every time；Merge extract solution, filter
American cockroach polypide ethanol extract；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.06MPa, temperature 60 C, is to proportion
When 1.15, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 10 times of amounts of its weight, warming while stirring D,
To 95 DEG C, then constant temperature stirs 60 minutes, and mixing speed is 60 revs/min, and then insulation stands 5 hours and is allowed to be layered；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
1.0 times of amount medicinal carbons of gained ethanol concentrate stir 60 minutes at a temperature of 60 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 30 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 60 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of temperature 60 C, vacuum -0.06MPa, being concentrated into proportion is
When 1.30, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G under conditions of 60 DEG C of temperature, vacuum -0.07MPa
Vacuum drying, crush as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment lung cancer for extracting to obtain using income approach of the present invention
0.7%, total amino acid content 71.4%, with reference to amino acid content 57.8%.
30 cases of lung cancer, man 15, female 15 are age 36-64 year, average（42±2.5）Year, the course of disease at -5 years 14 months,
Average course of disease：37 ± 5.8 months.
Auxiliary curative effect standard（1）It is effective：After 1st month terminates, patient body immunocompetence reduces seldom, and tumour cell is not
Further growth, transfer；After 3rd month terminates, patient body immunocompetence reduces seldom, the non-further growth of tumour cell,
Transfer；After 6th month terminates, patient body immunocompetence is reduced seldom, and tumour cell has been reduced；After 12nd month terminates,
Before patient body immunocompetence returns to radiotherapy, tumour cell is further reduced, and respiratory function recovers normal；（3）Effectively：1st
After individual month terminates, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；Terminate within 3rd month
Afterwards, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；After 6th month terminates, patient
Body immune ability is not reduced seriously, and tumour cell has been reduced；After 12nd month terminates, patient body immunocompetence is recovered
To before radiotherapy, tumour cell is further reduced, and respiratory function returns to normal；（3）It is invalid：Cardinal symptom, sign are without obvious
Improve.
Auxiliary treating method one：Patient is receiving radiotherapy in lung cancer treatment.Controlling prepared by the oral the present embodiment step G of patient
The American-cockroach-extract medicinal extract of lung cancer is treated, takes American-cockroach-extract medicinal extract 0.2g to be added in 30ml warm water and stirs,
For one day dosage.Every natural gift morning, noon, evening take three times, each taking 10ml, even served 12 months.
Auxiliary treating method two：Patient is receiving radiotherapy in lung cancer treatment.It is auxiliary prepared by the oral the present embodiment step H of patient
The American-cockroach-extract finished product fine powder for the treatment of stomach cancer is helped, takes American-cockroach-extract medicinal extract 0.2g obtained by step G dried
Fine powder is added in 30ml warm water and stirred, and is one day dosage.Per natural gift morning, the noon, it is late take three times, each taking 10ml,
It even served 12 months.
The result of auxiliary treating method one：Effective 19 for the treatment of group, effective 8, invalid 3, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The result of auxiliary treating method two：Effective 19 for the treatment of group, effective 8, invalid 3, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to auxiliary treatment lung cancer, specific compatibility, preparation, instructions of taking are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, and its compatibility of drugs and preparation method are as follows：By oldenlandia diffusa 40g,
Red sage root 10g, curcuma zedoary 8g, feeding intake in proportion, add cold water soak 30 minutes, water did not had medicinal material 2-5cm, had soaked after intense fire boils,
Turn slow fire infusion 20 minutes, filter out the dregs of a decoction, decoct 2 times again, the treatment lung cancer use that step G is obtained is added after merging 3 concoctions
American-cockroach-extract medicinal extract 0.15g or the equivalent medicinal extract drying fine powder that produces to obtain by step H, be well mixed.This
Pharmaceutical quantities are one day dosage, and every natural gift morning, noon, evening take three times.
With Chinese medicine compatibility auxiliary treatment lung cancer result：
Auxiliary treating method three：Patient is receiving radiotherapy in lung cancer treatment.The treatment lung cancer for adding step G to obtain after Chinese medicine extraction is used
American-cockroach-extract medicinal extract 0.15g, effective 19 for the treatment of group, effective 9, invalid 2, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
Auxiliary treating method four：Patient is receiving radiotherapy in lung cancer treatment.After Chinese medicine extraction plus 0.15g medicinal extract presses step H
Obtained drying fine powder is produced, effective 19 for the treatment of group, effective 9, invalid 2, in therapeutic process, patient has no bad anti-
Should, paid a return visit after half a year without recurrence.
Embodiment 3
The process for preparing medicine of extraction treatment lung cancer, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 20 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 75%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 8cm, closed to preserve 12 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：30 DEG C, ultrasonic power 20KHz of temperature, the ethanol of 5 times of overstriking powder weight amount concentration 75%, during extraction
Between be 30min；
Second of ultrasonic extraction：35 DEG C, ultrasonic power 23KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 75%, during extraction
Between be 20min；
Third time ultrasonic extraction：40 DEG C, ultrasonic power 25KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 75%, during extraction
Between be 35min；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.05MPa, temperature 50 C, is to proportion
When 1.05, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 45 minutes, mixing speed is 45 revs/min, and then insulation stands and is allowed to be layered for 4 hours.
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, added in gained filtrate
0.7 times of amount medicinal carbon of ethanol concentrate obtained by step C stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 45 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 55 DEG C of temperature, vacuum -0.06MPa, being concentrated into proportion is
When 1.25, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G under conditions of 55 DEG C of temperature, vacuum -0.07MPa
Vacuum drying, crush as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment lung cancer for extracting to obtain using income approach of the present invention
1.0%, total amino acid content 72.1%, with reference to amino acid content 58.0%.
Case：
30 cases of lung cancer, man 15, female 15 are age 38-54 year, average（45±4.5）Year, the course of disease are average at -7 years 10 months
The course of disease：51 ± 3.5 months.
Auxiliary curative effect standard（1）It is effective：After 2nd month terminates, patient body immunocompetence reduces seldom, and tumour cell is not
Further growth, transfer；After 4th month terminates, patient body immunocompetence reduces seldom, the non-further growth of tumour cell,
Transfer；After 8th month terminates, patient body immunocompetence is reduced seldom, and tumour cell has been reduced；After 12nd month terminates,
Before patient body immunocompetence returns to radiotherapy, tumour cell is further reduced, and respiratory function returns to normal；（3）Effectively：
After 2nd month terminates, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；4th month
After end, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；After 8th month terminates,
Patient body immunocompetence is not reduced seriously, and tumour cell has been reduced；After 12nd month terminates, patient body immunocompetence
Before returning to radiotherapy, tumour cell is further reduced, and respiratory function returns to normal；（3）It is invalid：Cardinal symptom, sign without
It is obviously improved.
Auxiliary treating method one：Patient is receiving lung cancer chemotherapy treatment.Controlling prepared by the oral the present embodiment step G of patient
The American-cockroach-extract medicinal extract of lung cancer is treated, takes American-cockroach-extract medicinal extract 0.2g to be added in 30ml warm water and stirs,
For one day dosage.Every natural gift morning, noon, evening take three times, each taking 10ml, even served 12 months.
Auxiliary treating method two：Patient is receiving lung cancer chemotherapy treatment.It is auxiliary prepared by the oral the present embodiment step H of patient
The American-cockroach-extract finished product fine powder for the treatment of stomach cancer is helped, takes American-cockroach-extract medicinal extract 0.2g obtained by step G dried
Fine powder is added in 30ml warm water and stirred, and is one day dosage.Per natural gift morning, the noon, it is late take three times, each taking 10ml,
It even served 12 months.
The result of auxiliary treating method one：Effective 18 for the treatment of group, effective 7, invalid 5, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The result of auxiliary treating method two：Effective 18 for the treatment of group, effective 7, invalid 5, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to auxiliary treatment lung cancer, specific compatibility, preparation, instructions of taking are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, and its compatibility of drugs and preparation method are as follows：By oldenlandia diffusa 50g,
Red sage root 20g, curcuma zedoary 12g feed intake in proportion, add cold water soak 30 minutes, and water did not had medicinal material 2-5cm, have soaked after intense fire boils,
Turn slow fire infusion 20 minutes, filter out the dregs of a decoction, decoct 2 times again, the treatment lung cancer use that step G is obtained is added after merging 3 concoctions
American-cockroach-extract medicinal extract 0.25g or the equivalent medicinal extract drying fine powder that produces to obtain by step H, be well mixed.This
Pharmaceutical quantities are one day dosage, and every natural gift morning, noon, evening take three times.
With Chinese medicine compatibility auxiliary treatment lung cancer result：
Auxiliary treating method three：Patient is receiving lung cancer chemotherapy treatment.The treatment lung cancer for adding step G to obtain after Chinese medicine extraction is used
American-cockroach-extract medicinal extract 0.25g, effective 18 for the treatment of group, effective 10, invalid 2, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
Auxiliary treating method four：Patient is receiving lung cancer chemotherapy treatment.After Chinese medicine extraction plus 0.25g medicinal extract presses step H
Obtained drying fine powder is produced, effective 18 for the treatment of group, effective 10, invalid 2, in therapeutic process, patient has no bad anti-
Should, paid a return visit after half a year without recurrence.
Embodiment 4
The process for preparing medicine of extraction treatment lung cancer, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 30 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 85%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 10cm, closed to preserve 10 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：35 DEG C, ultrasonic power 25KHz of temperature, the ethanol of 8 times of overstriking powder weight amount concentration 85%, during extraction
Between be 60min；
Second of ultrasonic extraction：40 DEG C, ultrasonic power 28KHz of temperature, the ethanol of 6 times of overstriking powder weight amount concentration 85%, during extraction
Between be 50min；
Third time ultrasonic extraction：Temperature 50 C, ultrasonic power 30KHz, the ethanol of 6 times of overstriking powder weight amount concentration 85%, during extraction
Between be 50min；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.06MPa, temperature 60 C, is to proportion
When 1.15, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 10 times of amounts of its weight, warming while stirring D,
To 90 DEG C, then constant temperature stirs 60 minutes, and mixing speed is 60 revs/min, and then insulation stands 5 hours and is allowed to be layered.
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, added in gained filtrate
0.8 times of amount medicinal carbon of filtrate stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 55 DEG C of temperature, vacuum -0.05MPa, is concentrated into ratio
When weight is 1.20, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G under conditions of 55 DEG C of temperature, vacuum -0.05MPa
Vacuum drying, crush as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment lung cancer for extracting to obtain using income approach of the present invention
0.9%, total amino acid content 66.3%, with reference to amino acid content 51.7%.
Case：
30 cases of lung cancer, man 18, female 12 are age 40-64 year, average（55±1.5）Year, the course of disease are average at -5 years 20 months
The course of disease：48 ± 5.6 months.
Auxiliary curative effect standard（1）It is effective：After 1st month terminates, wound recovers preferable after corrective surgery, stomach and intestine and digestion work(
It can recover normal, body immune ability is restored, the non-further growth of residual tumor cells, transfer；After 3rd month terminates,
Patient body immunocompetence gets a promotion, the non-further growth of residual tumor cells, transfer；After 6th month terminates, patient body
Immunocompetence recovers normal, and residual tumor cells have been reduced；After 12nd month terminates, patient body immunocompetence is normal, residual
Remaining tumour cell is further reduced, and Lung Cancer Recurrence rate is less than 40%；（3）Effectively：After 2nd month terminates, wound after corrective surgery
Recover preferable, stomach and intestine and digestive function recover normal, and body immune ability is restored, and residual tumor cells are not given birth to further
Long, transfer；After 5th month terminates, patient body immunocompetence gets a promotion, the non-further growth of residual tumor cells, transfer；
After 8th month terminates, patient body immunocompetence recovers normal, and residual tumor cells have been reduced；After 12nd month terminates, suffer from
Person's body immune ability is normal, and residual tumor cells are further reduced, and Lung Cancer Recurrence rate is less than 45%；（3）It is invalid：Main disease
Shape, sign are not improved.
Auxiliary treating method one：Patient received Lung Cancer Cases After Resection operation before the treatment, and Post operation is oral the present embodiment step
The American-cockroach-extract medicinal extract for the treatment of lung cancer prepared by rapid G, takes American-cockroach-extract medicinal extract 0.2g to be added to 30ml temperature
Stirred in water, be one day dosage.Every natural gift morning, noon, evening take three times, each taking 10ml, even served 12 months.
Auxiliary treating method two：Patient received Lung Cancer Cases After Resection operation before the treatment, and Post operation is oral the present embodiment step
The American-cockroach-extract finished product fine powder of auxiliary treatment stomach cancer prepared by rapid H, takes American-cockroach-extract medicinal extract obtained by step G
The dried fine powders of 0.2g, which are added in 30ml warm water, to stir, and is one day dosage.Every natural gift morning, noon, evening take three times, often
It is secondary to take 10ml, it even served 12 months.
The result of auxiliary treating method one：Effective 21 for the treatment of group, effective 7, invalid 2, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The result of auxiliary treating method two：Effective 21 for the treatment of group, effective 7, invalid 2, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to auxiliary treatment lung cancer, specific compatibility, preparation, instructions of taking are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, and its compatibility of drugs and preparation method are as follows：By oldenlandia diffusa 42g,
Red sage root 12g, curcuma zedoary 9g feed intake in proportion, add cold water soak 30 minutes, and water did not had medicinal material 2-5cm, have soaked after intense fire boils, have turned
Slow fire infusion 20 minutes, filters out the dregs of a decoction, decocts 2 times again, and the treatment lung cancer that step G is obtained is added after merging 3 concoctions
The drying fine powder that American-cockroach-extract medicinal extract 0.18g or equivalent medicinal extract are produced to obtain by step H, it is well mixed.This medicine
Dosage is one day dosage, and every natural gift morning, noon, evening take three times.
With Chinese medicine compatibility auxiliary treatment lung cancer result：
Auxiliary treating method three：Patient received Lung Cancer Cases After Resection operation before the treatment, and after Chinese medicine extraction plus what step G was obtained controls
The American-cockroach-extract medicinal extract 0.18g for the treatment of lung cancer, effective 21 for the treatment of group, effective 8, invalid 1, in therapeutic process,
Patient has no adverse reaction, is paid a return visit after half a year without recurrence.
Auxiliary treating method four：Patient received Lung Cancer Cases After Resection operation before the treatment, added 0.18g medicinal extract after Chinese medicine extraction
The drying fine powder for producing to obtain by step H, effective 21 for the treatment of group, effective 8, invalid 1, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
Embodiment 5
The process for preparing medicine of extraction treatment lung cancer, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 30 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 90%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 5cm, closed to preserve 3 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：30 DEG C, ultrasonic power 20KHz of temperature, the ethanol of 5 times of overstriking powder weight amount concentration 90%, during extraction
Between be 355min；
Second of ultrasonic extraction：35 DEG C, ultrasonic power 23KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 90%, during extraction
Between be 35min；
Third time ultrasonic extraction：43 DEG C, ultrasonic power 28KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 90%, during extraction
Between be 45min；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.05MPa, temperature 50 C, is to proportion
When 1.05, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 10 minutes, mixing speed is 20 revs/min, and then insulation stands and is allowed to be layered for 1 hour；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, filtrate is added in gained filtrate
0.5 times of amount medicinal carbon stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
G, elution filtrate obtained by step F is depressurized under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa dense
Contracting, be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G 50 DEG C~60 DEG C temperature, vacuum -0.04~-
It is dried in vacuo under conditions of 0.07MPa, crushes as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment lung cancer for extracting to obtain using income approach of the present invention
1.7%, total amino acid content 68.2%, with reference to amino acid content 56.1%.
Case：
30 cases of lung cancer, man 20, female 10 are age 40-76 year, average（50±7.5）Year, the course of disease are average at -5 years 20 months
The course of disease：44 ± 7.5 months.
Auxiliary curative effect standard（1）It is effective：After 1st month terminates, patient body immunocompetence reduces seldom, and tumour cell is not
Further growth, transfer；After 3rd month terminates, patient body immunocompetence reduces seldom, the non-further growth of tumour cell,
Transfer；After 6th month terminates, patient body immunocompetence is reduced seldom, and tumour cell has been reduced；After 12nd month terminates,
Before patient body immunocompetence returns to radiotherapy, tumour cell is further reduced, and respiratory function recovers normal；（3）Effectively：1st
After individual month terminates, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；Terminate within 3rd month
Afterwards, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；After 6th month terminates, patient
Body immune ability is not reduced seriously, and tumour cell has been reduced；After 12nd month terminates, patient body immunocompetence is recovered
To before radiotherapy, tumour cell is further reduced, and respiratory function returns to normal；（3）It is invalid：Cardinal symptom, sign are without obvious
Improve.
Auxiliary treating method one：Patient is receiving radiotherapy in lung cancer treatment.Controlling prepared by the oral the present embodiment step G of patient
The American-cockroach-extract medicinal extract of lung cancer is treated, takes American-cockroach-extract medicinal extract 0.2g to be added in 30ml warm water and stirs,
For one day dosage.Every natural gift morning, noon, evening take three times, each taking 10ml, even served 12 months.
Auxiliary treating method two：Patient is receiving radiotherapy in lung cancer treatment.It is auxiliary prepared by the oral the present embodiment step H of patient
The American-cockroach-extract finished product fine powder for the treatment of stomach cancer is helped, takes American-cockroach-extract medicinal extract 0.2g obtained by step G dried
Fine powder is added in 30ml warm water and stirred, and is one day dosage.Per natural gift morning, the noon, it is late take three times, each taking 10ml,
It even served 12 months.
The result of auxiliary treating method one：Effective 20 for the treatment of group, effective 8, invalid 2, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The result of auxiliary treating method two：Effective 20 for the treatment of group, effective 8, invalid 2, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to auxiliary treatment lung cancer, specific compatibility, preparation, instructions of taking are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, and its compatibility of drugs and preparation method are as follows：By oldenlandia diffusa 48g,
Red sage root 18g, curcuma zedoary 11g feed intake in proportion, add cold water soak 30 minutes, and water did not had medicinal material 2-5cm, have soaked after intense fire boils,
Turn slow fire infusion 20 minutes, filter out the dregs of a decoction, decoct 2 times again, the treatment lung cancer use that step G is obtained is added after merging 3 concoctions
American-cockroach-extract medicinal extract 0.22g or the equivalent medicinal extract drying fine powder that produces to obtain by step H, be well mixed.This
Pharmaceutical quantities are one day dosage, and every natural gift morning, noon, evening take three times.
With Chinese medicine compatibility auxiliary treatment lung cancer result：
Auxiliary treating method three：Patient is receiving radiotherapy in lung cancer treatment.The treatment lung cancer for adding step G to obtain after Chinese medicine extraction is used
American-cockroach-extract medicinal extract 0.22g, effective 21 for the treatment of group, effective 8, invalid 1, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
Auxiliary treating method four：Patient is receiving radiotherapy in lung cancer treatment.After Chinese medicine extraction plus 0.22g medicinal extract presses step H
Obtained drying fine powder is produced, effective 21 for the treatment of group, effective 8, invalid 1, in therapeutic process, patient has no bad anti-
Should, paid a return visit after half a year without recurrence.
Embodiment 6
The process for preparing medicine of extraction treatment lung cancer, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 20 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 90%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 10cm, closed to preserve 15 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：32 DEG C, ultrasonic power 22KHz of temperature, the ethanol of 8 times of overstriking powder weight amount concentration 90%, during extraction
Between be 45min；
Second of ultrasonic extraction：38 DEG C, ultrasonic power 23-25KHz of temperature, the ethanol of 6 times of amount concentration 90% of overstriking powder weight, is carried
It is 45min to take the time；
Third time ultrasonic extraction：Temperature 45 C, ultrasonic power 28-30KHz, the ethanol of 6 times of amount concentration 90% of overstriking powder weight, carry
It is 50min to take the time；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.06MPa, temperature 60 C, is to proportion
When 1.15, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 8 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 50 minutes, mixing speed is 50 revs/min, and then insulation stands and is allowed to be layered for 5 hours.
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, added in gained filtrate
0.6 times of amount medicinal carbon of ethanol concentrate obtained by step C stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa,
Be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of lung cancer obtained by step G 50 DEG C~60 DEG C temperature, vacuum -0.04~-
It is dried in vacuo under conditions of 0.07MPa, crushes as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment lung cancer for extracting to obtain using income approach of the present invention
1.2%, total amino acid content 69.5%, with reference to amino acid content 52.8%.
Case：
30 cases of lung cancer, man 15, female 15 are age 37-68 year, average（42±2.5）Year, the course of disease are average at -4 years 12 months
The course of disease：30 ± 5.5 months.
Auxiliary curative effect standard（1）It is effective：After 2nd month terminates, patient body immunocompetence reduces seldom, and tumour cell is not
Further growth, transfer；After 4th month terminates, patient body immunocompetence reduces seldom, the non-further growth of tumour cell,
Transfer；After 8th month terminates, patient body immunocompetence is reduced seldom, and tumour cell has been reduced；After 12nd month terminates,
Before patient body immunocompetence returns to radiotherapy, tumour cell is further reduced, and respiratory function returns to normal；（3）Effectively：
After 2nd month terminates, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；4th month
After end, patient body immunocompetence does not reduce seriously, the non-further growth of tumour cell, transfer；After 8th month terminates,
Patient body immunocompetence is not reduced seriously, and tumour cell has been reduced；After 12nd month terminates, patient body immunocompetence
Before returning to radiotherapy, tumour cell is further reduced, and respiratory function returns to normal；（3）It is invalid：Cardinal symptom, sign without
It is obviously improved.
Auxiliary treating method one：Patient is receiving lung cancer chemotherapy treatment.Controlling prepared by the oral the present embodiment step G of patient
The American-cockroach-extract medicinal extract of lung cancer is treated, takes American-cockroach-extract medicinal extract 0.2g to be added in 30ml warm water and stirs,
For one day dosage.Every natural gift morning, noon, evening take three times, each taking 10ml, even served 12 months.
Auxiliary treating method two：Patient is receiving lung cancer chemotherapy treatment.It is auxiliary prepared by the oral the present embodiment step H of patient
The American-cockroach-extract finished product fine powder for the treatment of stomach cancer is helped, takes American-cockroach-extract medicinal extract 0.2g obtained by step G dried
Fine powder is added in 30ml warm water and stirred, and is one day dosage.Per natural gift morning, the noon, it is late take three times, each taking 10ml,
It even served 12 months.
The result of auxiliary treating method one：Effective 22 for the treatment of group, effective 7, invalid 1, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The result of auxiliary treating method two：Effective 22 for the treatment of group, effective 7, invalid 1, in therapeutic process, patient has no
Adverse reaction, paid a return visit after half a year without recurrence.
The American-cockroach-extract medicinal extract for the treatment lung cancer for extracting to obtain using the above method dries fine powder, in following
Medicinal material is compatible, has unique curative effect to auxiliary treatment lung cancer, specific compatibility, preparation, instructions of taking are as follows：
A kind of medicine for treating lung cancer, is counted in parts by weight, and its compatibility of drugs and preparation method are as follows：By oldenlandia diffusa 48g,
Red sage root 18g, curcuma zedoary 11g feed intake in proportion, add cold water soak 30 minutes, and water did not had medicinal material 2-5cm, have soaked after intense fire boils,
Turn slow fire infusion 20 minutes, filter out the dregs of a decoction, decoct 2 times again, the treatment lung cancer use that step G is obtained is added after merging 3 concoctions
American-cockroach-extract medicinal extract 0.2g or the equivalent medicinal extract drying fine powder that produces to obtain by step H, be well mixed.This medicine
Dosage is one day dosage, and every natural gift morning, noon, evening take three times.
With Chinese medicine compatibility auxiliary treatment lung cancer result：
Auxiliary treating method three：Patient is receiving lung cancer chemotherapy treatment.The treatment lung cancer for adding step G to obtain after Chinese medicine extraction is used
American-cockroach-extract medicinal extract 0.2g, effective 23 for the treatment of group, effective 7, invalid 0, in therapeutic process, patient has no not
Good reaction, paid a return visit after half a year without recurrence.
Auxiliary treating method four：Patient is receiving lung cancer chemotherapy treatment.After Chinese medicine extraction plus 0.2g medicinal extract is given birth to by step H
Obtained drying fine powder is produced, effective 23 for the treatment of group, effective 7, invalid 0, in therapeutic process, patient has no adverse reaction,
Paid a return visit after half a year without recurrence.
Claims (10)
1. a kind of process for preparing medicine for treating lung cancer, it is characterised in that prepared according to following steps：
A, by dry American cockroach polypide cross 10-40 mesh sieve crush, by gained American cockroach coarse powder concentration be 60%~
95% ethanol immersion, ethanol liquid level is set to be higher by 5~10cm of coarse powder liquid level, closed preservation one is more than week, less than 18 months,
Coarse powder mixed liquor is made；
B, coarse powder mixed liquor obtained by step A is subjected to 3~5 ultrasonic extractions at a temperature of 30~50 DEG C, ultrasonic power is：
20-30KHz, when to coarse powder mixed liquor extract for the first time, the ethanol of 5~8 times of amount concentration 60%~95% of overstriking powder weight, carry
It is 30~60min to take the time；The ethanol of 3~6 times of amount concentration 60%~95% of overstriking powder weight, extraction are extracted after second every time
Time is 2~50min；Merge extract solution, filter to obtain American cockroach polypide ethanol extract；
C, ethanol extract obtained by step B is depressurized under conditions of vacuum -0.05~-0.06MPa, 50~60 DEG C of temperature dense
Contracting, to proportion be 1.05~1.15 when, obtain American cockroach polypide ethanol concentrate；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7~10 times of amounts of its weight, rise while stirring D,
Temperature is to 85~95 DEG C, then constant temperature stirs 10~60 minutes, and mixing speed is 20~60 revs/min, and then insulation stands 1~5 hour
It is allowed to be layered；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
0.5 ~ 1.0 times of amount medicinal carbon of gained ethanol concentrate stirs 30 ~ 60 minutes at a temperature of 40 ~ 60 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first steamed with 10 ~ 30 times of amounts of medicinal carbon
Distilled water is washed, and water lotion discards；Again with the eluent of 30 ~ 60 times of amounts of medicinal carbon, eluent is collected, through filter paper mistake
Filter, must elute filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa,
Be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of lung cancer must be treated；
H, by the American-cockroach-extract for the treatment of hepatitis B obtained by step G in 50 DEG C~60 DEG C temperature, vacuums -0.04
It is dried in vacuo under conditions of~-0.07MPa, crushes as fine powder, that is, obtain the American-cockroach-extract finished product for treating lung cancer.
2. a kind of process for preparing medicine for treating lung cancer according to claim 1, it is characterised in that step B is according to following
Method is extracted, and coarse powder mixed liquor obtained by step A is carried out into ultrasonic extraction 3 times：
First time ultrasonic extraction：30~35 DEG C, ultrasonic power 20-25KHz of temperature, 5~8 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 30~60min；
Second of ultrasonic extraction：35~40 DEG C, ultrasonic power 23-28KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 20~50min；
Third time ultrasonic extraction：40~50 DEG C, ultrasonic power 25-30KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 35~50min.
3. a kind of process for preparing medicine for treating lung cancer according to claim 2, it is characterised in that in step B, for the first time
Ultrasonic extraction：30~32 DEG C, ultrasonic power 20-22KHz of temperature, the ethanol of 5~8 times of amount concentration 60%~95% of overstriking powder weight,
Extraction time is 35~45min.
A kind of 4. process for preparing medicine for treating lung cancer according to claim 2, it is characterised in that in step B, second
Ultrasonic extraction：35~38 DEG C, ultrasonic power 23-25KHz of temperature, the ethanol of 3~6 times of amount concentration 75%~95% of overstriking powder weight,
Extraction time is 35~45min.
A kind of 5. process for preparing medicine for treating lung cancer according to claim 2, it is characterised in that in step B, third time
Ultrasonic extraction：43~45 DEG C, ultrasonic power 28-30KHz of temperature, 3~6 times of amount concentration 80-95% of overstriking powder weight ethanol, is carried
It is 45~50min to take the time.
A kind of 6. process for preparing medicine for treating lung cancer according to claim 1, it is characterised in that step D：By step C
Gained American cockroach polypide ethanol concentrate adds the purified waters of 7~10 times of its weight amount, warming while stirring to 85~90 DEG C,
Constant temperature stirs 45~60 minutes again, and mixing speed is 45~60 revs/min, and then insulation stands 4~5 hours and is allowed to be layered.
7. a kind of process for preparing medicine for treating lung cancer according to claim 1, it is characterised in that in step E, by step
Lower floor's aqueous is released obtained by D, collects lower floor's aqueous, and after filter paper filters, 0.5-0.8 times of filtrate of addition measures medicine in gained filtrate
Stirred 45 minutes at a temperature of 50 DEG C with activated carbon, it is fully adsorbed.
8. a kind of process for preparing medicine for treating lung cancer according to claim 1, it is characterised in that in step F, by step
Decoction containing activated carbon obtained by E is fitted into chromatographic column, releases decoction, first with 20 times of amount distillation water washings of medicinal carbon, water lotion
Discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, is filtered through filter paper, filtrate must be eluted, it is described to wash
It is water that proportioning is counted in de- agent in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
9. a kind of medicine for treating lung cancer, it is characterised in that count, be formulated by following raw material in parts by weight：Oldenlandia
Careless 40-50 parts, the treatment that any one method and step G is prepared in red sage root 10-20 parts, curcuma zedoary 8-12 part claims 1-8
The drying fine powder that American-cockroach-extract medicinal extract 0.15-0.25 parts of lung cancer or equivalent medicinal extract are produced to obtain by step H.
10. a kind of medicine for treating lung cancer according to claim 9, it is characterised in that count in parts by weight, by following original
Material is formulated：Oldenlandia diffusa 42-48 parts, any one method in red sage root 12-18 parts, curcuma zedoary 9-11 part claims 1-8
The American-cockroach-extract medicinal extract 0.18-0.22 parts for the treatment lung cancer that step G is prepared or equivalent medicinal extract are produced by step H
Obtained drying fine powder.
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| CN201710226210 | 2017-04-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109674824A (en) * | 2019-02-15 | 2019-04-26 | 昆明赛诺制药股份有限公司 | It is a kind of to treat phthisical drug and preparation method thereof |
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