CN107684433B - device and method for simultaneously detecting multiple physiological parameter indexes of wound surface - Google Patents
device and method for simultaneously detecting multiple physiological parameter indexes of wound surface Download PDFInfo
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- CN107684433B CN107684433B CN201710607266.6A CN201710607266A CN107684433B CN 107684433 B CN107684433 B CN 107684433B CN 201710607266 A CN201710607266 A CN 201710607266A CN 107684433 B CN107684433 B CN 107684433B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/14539—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring pH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/14546—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring analytes not otherwise provided for, e.g. ions, cytochromes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
- A61B5/14551—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters for measuring blood gases
- A61B5/14552—Details of sensors specially adapted therefor
Abstract
the invention discloses a device for detecting various physiological parameter indexes of a wound surface, which comprises: the device comprises a reflective photoelectric detection module, a sensitive film sensitive to the physiological parameters of the wound surface and a transparent isolation layer film positioned between the sensitive film and a photoelectric device; the reflective photoelectric detection module comprises a photoelectric receiving tube, n Light Emitting Diodes (LEDs) and a drive circuit. The n sensitive films which are in one-to-one correspondence with the LEDs with different wavelengths are arranged according to the positions of the reflection light paths in one-to-one correspondence, the photoelectric detection of a plurality of physiological parameters is multiplexed, the light with different wavelengths can be utilized to detect a plurality of physiological parameters of the wound surface in a time-sharing manner, and the in-situ on-line detection of the plurality of physiological parameters is realized.
Description
Technical Field
The invention belongs to the field of biomedical engineering, and particularly relates to a device and a method for realizing time-sharing in-situ online detection of various physiological parameters of a wound surface by using light with different wavelengths.
Background
The number of surgical patients is about 6500 ten thousand per year in China, and the bandage technology for wound treatment is very common in application. For surgical patients, one of the important problems faced is the problem of wound healing after surgery. The method is simple and easy to implement, but once the wound is wrapped, a doctor cannot monitor the growth state of the wound in situ in real time and cannot treat inflammation and the like in real time, the wound healing is a long time, the wound cannot be accurately observed and diagnosed in the long time, the skin healing is about 10-20 days even though the operation is performed on the surface of the skin, and the wound cannot be treated in time if early inflammation or other problems of the wound occur in the long time. For example, infection of a wound surface not only results in delayed healing of the wound surface, but also seriously endangers the life of the patient. In clinical diagnosis, invasive gold standard method is adopted in early stage, although the measurement is accurate, the gauze needs to be uncovered continuously to detect the wound surface state, the secondary injury of the wound is caused, the risk of secondary infection is increased, and the method has the defects of high price, time consumption, incapability of continuous measurement and the like. Therefore, the biosensor sensitive to the wound infection marker (such as PH) is used for detecting the wound condition, and has important clinical guiding significance.
Based on this, electronic bandage technology has been proposed. The invention multiplexes photoelectric detection of a plurality of physiological parameters, can detect a plurality of physiological parameters including arterial blood oxygen saturation, PH value, lactic acid content and the like by utilizing light with different wavelengths in a time-sharing way, and realizes the function of detecting a plurality of physiological parameters of a wound surface in situ and on line by a tiny device.
Disclosure of Invention
In view of the above, the present invention provides a device for simultaneously detecting multiple physiological parameter indexes of a wound, by which in-situ online detection of multiple physiological parameters of the wound, including arterial oxygen saturation, PH, lactic acid content, etc., can be achieved.
The technical scheme of the invention is as follows:
A device for simultaneously detecting multiple physiological parameter indexes of a wound surface is characterized by comprising:
The device comprises a reflection type photoelectric detection module, a photoelectric detection module and a control module, wherein the reflection type photoelectric detection module at least comprises a photoelectric receiving tube, n Light Emitting Diodes (LEDs) with different wavelengths and a driving circuit for controlling the LEDs;
sensitive film sensitive to physiological parameters of the wound surface; and the number of the first and second groups,
And the transparent isolating layer film is positioned between the sensitive film and the reflective photoelectric detection module.
The working principle is as follows: the n Light Emitting Diodes (LEDs) emit laser signals with different wavelengths in a time-sharing manner, the optical signals irradiate the surface of the sensitive film through the transparent isolation layer film, the sensitive film changes color due to the change of physiological parameters, the sensitive film has different absorbances for different concentrations of physiological parameters of a certain wound surface under fixed wavelength light and reflects laser with different wavelengths, reflected light enters the photoelectric receiving tube through the transparent isolation layer film, the photoelectric receiving tube generates optical current or voltage after receiving the optical signals, and the intensity of the optical signals with different wavelengths is obtained by calculating the size of the optical current or voltage, so that the size change of the physiological parameters is obtained.
Preferably, the transparent isolation layer film is a transparent film with light transmittance of more than 70% and biocompatibility. Further preferably, the transparent isolating layer film is polydimethylsiloxane PDMS or polylactic acid PLA. The transparent isolating layer film prevents the wound surface liquid from corroding the reflective photoelectric detection module and has a ventilation effect.
Preferably, the thickness of the isolating layer film is 90-110 μm.
Preferably, the sensitive film is spin-coated on the transparent isolating layer film, and the sensitive film is in contact with the wound surface during working, so that the sensing of the physiological parameters of the wound surface is realized.
Preferably, the thickness of the sensitive film is 1-30 μm.
Preferably, the sensitive film is a pH sensitive film, a glucose sensitive film, a lactic acid sensitive film, a chloride ion sensitive film or a metal ion sensitive film.
Preferably, the device comprises n kinds of sensitive films corresponding to the light emitting diodes with different wavelengths one by one, and each kind of sensitive film reflects the light emitted by the corresponding light emitting diode to the photoelectric receiving tube. The light emitting diode LED corresponding to the sensitive film is determined by performing an absorbance test on the sensitive film through an ultraviolet-visible spectrophotometer, and the absorbance resolution of the sensitive film is the highest under the illumination of the light emitting diode LED. Therefore, various physiological parameters of the wound surface can be detected quickly and accurately at the same time.
the device is used for multiplexing photoelectric detection of a plurality of physiological parameters, detecting the plurality of physiological parameters of the wound surface by using light with different wavelengths in a time-sharing manner, and realizing in-situ on-line detection of the plurality of physiological parameters.
Compared with the prior art, the invention has the beneficial effects that: the n sensitive films corresponding to the LEDs with different wavelengths one to one are distributed according to the positions corresponding to the reflection light paths one to one, and the light with different wavelengths is used for time division detection of various physiological parameters of the wound surface, so that the purpose of simultaneously detecting various physiological parameter indexes of the wound surface can be realized. The biosensor is in line with the development direction of future biosensors, namely miniaturized multifunction and does not bring discomfort to people.
drawings
Fig. 1 is a schematic structural diagram of a device for simultaneously detecting multiple physiological parameter indexes of a wound surface, which is attached to the surface of the wound surface and provided in embodiment 1;
Fig. 2 is a schematic structural diagram of the device for simultaneously detecting multiple physiological parameter indexes of a wound surface, which is attached to the surface of the wound surface and provided in embodiment 2.
FIG. 3 is a graph of a distribution of blood oxygen saturation values measured using the apparatus provided in example 2;
FIG. 4 is a pH profile measured using the apparatus provided in example 2;
FIG. 5 is a graph of a distribution of blood oxygen saturation values measured when the reflective blood oxygen sensor provided in example 2 is not coated with a sensitive film.
Detailed Description
In order to more specifically describe the present invention, the following detailed description is provided for the technical solution of the present invention with reference to the accompanying drawings and the specific embodiments.
Example 1
Fig. 1 is a schematic structural view of a device for simultaneously detecting multiple physiological parameter indexes of a wound surface, which is attached to the surface of the wound surface according to an embodiment of the present invention. The method comprises the following steps: the device comprises a reflection type photoelectric detection module 1, a transparent isolation layer film 2 coated on the surface of the reflection type photoelectric detection module 1, a PH sensitive film 31, a lactic acid sensitive film 32, a glucose sensitive film 33 and a wound surface 8, wherein the PH sensitive film 31, the lactic acid sensitive film 32, the glucose sensitive film 33 and the wound surface are spin-coated on the transparent isolation layer film 2. The reflective photoelectric detection module 1 includes LEDs 4, 5, LEDs 6 and photodiodes 7 with optimal wavelengths, which are corresponding to the sensitive films one to one.
example 2
In this embodiment, only one PH sensitive film is spin-coated on the transparent isolation layer film, and the PH sensitive film is completely coated on the transparent isolation layer film. As shown in fig. 2, the apparatus provided in this embodiment includes: the blood oxygen sensor comprises a reflective blood oxygen sensor 1 with the model of SFH7050, PDMS 2 coated on the surface of the reflective blood oxygen sensor 1, a PH sensitive film 3 spin-coated on the PDMS 2 and a wound surface 8. The reflective blood oxygen sensor 1 comprises a red LED4, a green LED5, an infrared LED6 and a photodiode 7.
The PH sensitive film 3 is prepared by coating a sol-gel precursor with acid-base indicator dye molecules by adopting a sol-gel technology. Wherein, the acid-base indicator dye molecules comprise bromocresol green, bromocresol green sodium salt, methyl red and neutral red.
Sol-gel precursors are classified into three types, inorganic precursors including Tetraethylorthosilicate (TEOS), Tetramethoxysilane (TMOS), organic precursors, and organic-inorganic hybrid precursors; the organic precursor comprises gamma-Glycidoxypropyltrimethoxysilane (GLYMO), Methyltriethoxysilane (MTES), ethyltriethoxysilane modification (ETES) or phenyltrimethoxysilane (FTMS); the organic-inorganic hybrid precursor is obtained by mixing an organic precursor and an inorganic precursor in different proportions.
The PH sensitive film 3 has a thickness of 10 μm, and the volume ratio of the organic precursor to the inorganic precursor is 3:7 (1 mL in total) for the PH sensitive film to achieve optimum results among mechanical properties, optical transparency, and response time. The preparation method comprises the following steps:
first, 1.2mL of ethanol, 16.3mg of bromocresol green sodium salt, 0.3mL of γ -Glycidoxypropyltrimethoxysilane (GLYMO), 0.7mL of Tetraethylorthosilicate (TEOS), and 0.4mL of a catalyst (0.1M HCl) were mixed to prepare a sol, which was then homogenized by ultrasonic bath for 10min, aged at room temperature for 3 days, then spin-coated onto PDMS by a spin-coating technique, and left to stand at room temperature in the dark for 3 days.
The light emitted by the LED in the reflective blood oxygen sensor SFH7050 is irradiated on a wound surface through the isolation layer film and the sensitive film, a scattering part and an absorption part are removed, the reflected light is received by the photodiode and converted into an electric signal in proportion to the light intensity, the blood oxygen saturation is obtained by utilizing an arterial blood oxygen saturation calculation formula, the absorption condition of the light when passing through tissues and blood vessels in the electric signal can be divided into a pulsating component (such as arterial blood) and a non-pulsating component (such as skin, tissues, venous blood and the like), namely, the electric signal can be called Alternating Current (AC) and Direct Current (DC), and the pH value of a wound does not have sudden change, so the absorption of the light by the pH sensitive film is used as a DC part.
The device prepared by the embodiment is placed on the surface of a wound surface, based on Lambert-beer law, an electric signal, namely a photoplethysmogram (PPG), is obtained by measuring two paths of LED light of red light and infrared light, and the blood oxygen saturation is obtained through an arterial blood oxygen saturation calculation formula, and the result is shown in figure 3.
The devices prepared in this example were placed in different pH buffers at pH 5-9 and spaced apart by 1, and the pH was characterized using an electrical signal measured from light emitted from a green LED, the results of which are shown in FIG. 4.
The reflective blood oxygen sensor provided by the embodiment is placed on the surface of a wound surface when the reflective blood oxygen sensor is not coated with the PH sensitive film in a spin mode, and the blood oxygen saturation degree is calculated by combining a PPG signal obtained by measuring red light and infrared light with the Lambert-beer law. The calculation formula is as follows:
[ HbO 2 ] and [ Hb ] indicate the concentrations of oxygenated hemoglobin and deoxygenated hemoglobin, respectively.
α (HbO 2, λ Red), α (HbO 2, λ IR), α (Hb, λ Red), α (Hb, λ IR) are HbO 2 and Hb pairs
Red and infrared absorption coefficients. R is calculated as follows:
According to the spectral absorption characteristics of HbO 2/Hb, the absorption coefficient satisfies the following relationship:
α(Hb,λRed)＞＞α(HbO2,λRed) (4)
α(Hb,λIR)≈α(HbO2,λIR) (5)
Therefore, equation (1) can be simplified as:
A and B were calibrated using a commercial photo-oximeter.
The blood oxygen saturation was calculated by the above formula, and the result is shown in fig. 5. Corresponding to each measurement result, the device for detecting the multiple physiological parameter indexes of the wound surface provided by the embodiment of the invention has only 0.1-0.2% difference with the blood oxygen saturation obtained by testing the non-spin-coated PH sensitive film. The results indicate that the presence of the PH sensitive membrane does not affect the measurement of blood oxygen saturation.
Therefore, the embodiment can realize in-situ online detection of the arterial blood oxygen saturation and the PH value of the wound.
The above-mentioned embodiments are intended to illustrate the technical solutions and advantages of the present invention, and it should be understood that the above-mentioned embodiments are only the most preferred embodiments of the present invention, and are not intended to limit the present invention, and any modifications, additions, equivalents, etc. made within the scope of the principles of the present invention should be included in the scope of the present invention.
Claims (6)
1. A device for simultaneously detecting multiple physiological parameter indexes of a wound surface is characterized by comprising:
The device comprises a reflection type photoelectric detection module, a photoelectric detection module and a control module, wherein the reflection type photoelectric detection module at least comprises a photoelectric receiving tube, n Light Emitting Diodes (LEDs) with different wavelengths and a driving circuit for controlling the LEDs;
The system comprises n sensitive films sensitive to the physiological parameters of the wound surface, wherein the n sensitive films correspond to n Light Emitting Diodes (LEDs) with different wavelengths one by one, and each sensitive film reflects light emitted by the corresponding LED to a photoelectric receiving tube; and the number of the first and second groups,
the transparent isolating layer film is positioned between the sensitive film and the reflective photoelectric detection module;
the sensitive film is coated on the transparent isolating layer film in a spinning mode, and the sensitive film is in contact with the wound surface when in work, so that the physiological parameters of the wound surface are sensed.
2. a device for simultaneously detecting multiple physiological parameter indicators of a wound as claimed in claim 1, wherein the transparent isolation layer film is a transparent film with a light transmittance of more than 70% and biocompatibility.
3. A device for simultaneously detecting multiple physiological parameter indicators of a wound as claimed in claim 2, wherein the transparent isolation layer film is polydimethylsiloxane PDMS or polylactic acid PLA.
4. a device for simultaneously detecting multiple physiological parameter indicators of a wound as claimed in claim 1, wherein the thickness of the sensitive film is 1 μm to 30 μm.
5. The device for simultaneously detecting multiple physiological parameter indexes of a wound surface according to claim 1, wherein the sensitive film is a PH sensitive film, a glucose sensitive film, a lactic acid sensitive film, a chloride ion sensitive film or a metal ion sensitive film.
6. A method for simultaneously detecting multiple physiological parameter indexes of a wound surface is characterized in that the device of any one of claims 1 to 5 is used for multiplexing photoelectric detection of multiple physiological parameters, light with different wavelengths is used for time division detection of multiple physiological parameters of the wound surface, and in-situ on-line detection of the multiple physiological parameters is realized.
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| CN111449641B (en) * | 2020-04-20 | 2021-07-20 | 浙江大学 | Evaluation device and evaluation method for muscle function state based on photoelectric signal detection |
| CN114098668B (en) * | 2020-08-31 | 2022-11-11 | 荣耀终端有限公司 | Living body detection method and electronic equipment |
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| GB2519335A (en) * | 2013-10-17 | 2015-04-22 | Univ Loughborough | Opto-physiological sensor and method of design |
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