CN107674837A - A kind of full automatic cells culture and analysis load - Google Patents
A kind of full automatic cells culture and analysis load Download PDFInfo
- Publication number
- CN107674837A CN107674837A CN201711033458.7A CN201711033458A CN107674837A CN 107674837 A CN107674837 A CN 107674837A CN 201711033458 A CN201711033458 A CN 201711033458A CN 107674837 A CN107674837 A CN 107674837A
- Authority
- CN
- China
- Prior art keywords
- module
- enrichment
- culture
- cell
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to cell injuring model technical field, more particularly to a kind of automatic cytological culture apparatus suitable for space Bioexperiment.A kind of full automatic cells culture and analysis load, its technical scheme are：It is located at including control unit, cell culture and analytic unit and observing unit inside load carrier；ECU is used to provide direct current to control unit, cell culture and analytic unit and observing unit；Cell culture and analytic unit are used to irrigate culture, enrichment and on-line analysis to the protide factor in nutrient solution to cell；Control unit integrates 1553B bus telemetry interfaces, for each component in cell culture and analytic unit to be monitored and controlled.The present invention realizes the cell perfusion culture of autonomous type and culture and analysis to the GAP-associated protein GAP class factor, and the structure design and circuit design of device meet the related request of the heat of space carrying environment, mechanical property and Electro Magnetic Compatibility.
Description
Technical field
The present invention relates to cell injuring model technical field, more particularly to a kind of automation suitable for space Bioexperiment
Cell culture apparatus.
Background technology
Cell injuring model technology is the basis of RESEARCH ON CELL-BIOLOGY, is occupied in biotechnology research field particularly significant
Position.Conventional cell culture cultivates cell using devices such as Tissue Culture Flask, CO2gas incubators, it is impossible to accurate weight
Now internal microenvironment, and it is not suitable for the prolonged online observation of cell.The nineties with year over microflow control technique development
New idea and method is provided for cell culture.The channel size of micro-fluidic chip is typically at tens to hundreds of microns, and carefully
The size of born of the same parents is roughly the same, convection body can be accurately controlled from room and time, saves substantial amounts of reagent and cell,
With the unrivaled advantage of conventional cell culture technique.Cell culture technology on micro-fluidic chip cell migration,
It is used widely in the fields such as drug screening, cell differentiation.
Two basic requirements with high flux for a long time for the cell culture on chip, in addition with controllability, can
Many requirements such as observation property.The operation such as real-time morphologic observation, biochemistry detection, study on microenvironment regulation is carried out in incubation
When often can all have the risk of pollution, while be also unfavorable for ensureing the repeatability and collimation of experiment, difficulty brought for experiment
And risk.The culture of automation can substantially reduce these risks and difficulty, and some domestic and international automations based on microchip are thin
Born of the same parents' culture apparatus is mostly bulky, power consumption is high, expensive, and is limited by processing request high condition, it is difficult to meet space
Carry etc. experimental situation to minimizing, automating, low-power consumption, high reliability etc. require.
The content of the invention
The purpose of the present invention is：There is provided one kind can under space and equal adverse circumstances autonomous control cell culture, egg
The constructional device of white class Factor Analysis and online observation.
The technical scheme is that：A kind of described full automatic cells culture and analysis load are provided, including：Automatically controlled list
Member, load carrier and control unit and cell culture and analytic unit inside the load carrier；
The ECU is used to provide direct current to described control unit and the cell culture and analytic unit；
The cell culture and analytic unit irrigate culture, to the richness of the protide factor in nutrient solution for carrying out cell
Collection and on-line analysis；Including：Syringe pump, media to cell culture modules, enrichment module, analysis module and waste collection module；
The syringe pump is used to nutrient solution being injected into the media to cell culture modules, and its injection speed is single by the control
Member control；
The media to cell culture modules is used to cultivate cell, enters trip temperature control to the media to cell culture modules by temperature control component
System, the temperature control component are controlled by described control unit；
The enrichment module is used for the cell factor detected needed for the nutrient solution that is flowed into from the media to cell culture modules
Carry out continuing enrichment；
The analysis module is used to detect the rho factor in the nutrient solution after enrichment module enrichment,
After completing detection, nutrient solution is emitted into the waste collection module.
Also include observing unit, the observing unit includes：Data acquisition unit and the first image-forming assembly；It is described the first one-tenth
As component by observation port below the media to cell culture modules to the cellular morphology in the media to cell culture modules, increment and migration
Situation is observed；First image-forming assembly includes：First CCD imaging plates, the microcobjective on the first CCD imaging plates
And the focusing structure for being focused to the microcobjective；The focusing structure is carried out certainly by described control unit control
Homophony is burnt；
The data acquisition unit is adopted under the control of described control unit to the image of first image-forming assembly
Collection, and the image of collection is compressed and stored.
The observing unit also includes being used for the second image-forming assembly for taking pictures to the testing result of the analysis module；
Second image-forming assembly includes：2nd CCD imaging plates, the tight shot on the 2nd CCD imaging plates；
The data acquisition unit is adopted under the control of described control unit to the image of second image-forming assembly
Collection, and the image of collection is compressed and stored.
The observing unit also includes being used for providing first image-forming assembly the first LED of illumination and for described
Second image-forming assembly provides the 2nd LED of illumination；The opening and closing of first LED and the 2nd LED is controlled by described control unit.
The media to cell culture modules is to engage the micro-fluidic of multi-chamber made of polyester web frame using PDMS or PVC material
Chip.
Beneficial effect：
(1) present invention can realize the cell perfusion culture of autonomous type and culture and analysis to the GAP-associated protein GAP class factor,
The structure design of device meets the related of the heat of space carrying environment, mechanical property and Electro Magnetic Compatibility to circuit design will
Ask.
(2) it can efficiently realize that the temperature stability, free switching perfusion rate, autonomous focusing of cell culture environment are clapped
According to effective power consumption demand for reducing long-time incubation.
(3) integrated cost of experiment of the invention and operation is low, physical size is suitable, and easy to process and sterilizing is simple, energy
The Continuous Observation of cell growth condition and active situation in incubation is enough realized, effectively avoids introducing experiment the correlation of pollution
Factor.
Brief description of the drawings
Fig. 1 is the block diagram of system of the present invention；
Fig. 2 is the structural representation of cell culture and analytic unit in the present invention；
Fig. 3 flows to schematic diagram for the fluid path of cell culture and analytic unit in the present invention；
Fig. 4 is the circuit system schematic diagram of the present invention；
Fig. 5 is the control software flow chart of the present invention；
Fig. 6 is the FPGA software block diagrams of observing unit in the present invention.
Embodiment
The present invention will now be described in detail with reference to the accompanying drawings and examples.
Referring to accompanying drawing 1, a kind of full automatic cells culture and analysis load, it includes：ECU 1, load carrier 2, control
Unit 3, cell culture and analytic unit 4 and observing unit 5 processed.
Control unit 3, cell culture and analytic unit 4 and observing unit 5 are located inside load carrier 2；Load carrier 2
Play a part of integrated support and good heat conductive, stable closed environment is provided for interior arrangement, while disclosure satisfy that space is taken
Carry the harsh mechanical requirements of experimental situation and mechanical interface requirement.
In this example, for load carrier 2 using duralumin 2A12-H112 as structural material, outward appearance is that cube body structure is maximum
Envelope 380mm × 270mm × 230mm, weight are less than 16kg；Body structure uses integral wall plank frame, and integral panel uses
Mechanical milling shaping, casing engagement surface chamfer.Two side cover plate end faces are sealed using fastening bolt mode；Body structure surface processing uses
Aluminium heat-tinting.The interface fixed with aircraft installation of load carrier 2 is designed using captive screw, is prevented when device dismounts
It is in the presence of that screw is inadvertently fallen off.Component, circuit board inside load carrier 2 are fixed on the bottom of casing using trip bolt
On plate or side cover plate, the big component of caloric value and circuit components are designed with conductive structure and casing in the course of the work for part
It is closely coupled.The external all electric interfaces of load carrier 2 use aerospace level electric connector, on side cover plate.Device
Internal fluid path and cable are fixed on wallboards of box body according to specific layout scenarios using line card and screw.Structure design with
It is optimization aim to reduce weight as far as possible and improve disassembly efficiency, while passes through heat emulation, Mechanics Simulation and later stage corresponding ring
Border experiment ensures heat, mechanical property and the Electro Magnetic Compatibility of structure.
ECU 1 is used to provide direct current to control unit 3, cell culture and analytic unit 4 and observing unit 5.
In this example, the outward appearance of ECU 1 is cube structure largest enveloping 302.5mm × 214mm × 210mm, and weight is less than 10kg；Referring to
Accompanying drawing 4, ECU 1 are internally provided with lithium battery 11 and power-switching circuit plate 12.Wherein power-switching circuit plate 12 uses
DC-DC electrical level transferring chips and relay realize the autonomous switching between aircraft platforms power supply and the power supply of standby lithium pond 11.
Lithium battery 11 nominal capacity 18Ah, the output voltage range 22V~33.6V produced according to space product Specification Design, support anti-
Multiple repeatedly charging, indoor design have management of charging and discharging circuit；The related performance indicators of lithium battery 11 meet environmental test requirement.Lithium electricity
Pond 11 is fixed on by trip bolt on the bottom plate of the casing of ECU 1, and its power supply interface is connected to power-switching circuit plate 12,
Charging inlet is connected to the electric connector on side cover plate, and outside can connect special charger and lithium battery is charged.
The isolated buck for the 100V buses that power-switching circuit plate 12 is used to complete to provide platform is changed, and is realized standby
The autonomous switching powered with lithium battery 11 and 100V.Wherein 100V buses input front end is designed with fuse protection circuit and surge suppression
Circuit processed, rear end are converted to 28V by DC/DC insulating power supply modules and exported.Design is realized from DC/DC insulating power supplies using relay
Switching between the 28V and lithium battery output voltage of module output.Relay switchs for SPDT, by Enable Pin to defeated
Go out end and realize switching.When 100V, which powers, to be connected, relay Enable Pin obtains driving current, switches to relay output end
The 28V of DC/DC insulating power supplies module output；When 100V, which powers, to be disconnected, relay output end automatically switches to lithium battery output
Voltage.
Referring to accompanying drawing 2,3, cell culture and analytic unit 4 are used for cell irrigation culture, to the protide in nutrient solution
The enrichment and on-line analysis of the factor；It includes：Syringe pump 41, media to cell culture modules 42, enrichment module 43, analysis module 44 with
And waste collection module 45.
In this example, support to complete one group of contrast test, use two media to cell culture modules, 42, two enrichment modules 43, two
Individual analysis module 44.
Syringe pump 41 is used to nutrient solution being injected into media to cell culture modules 42；Syringe pump 41 includes two groups of totally 8 20mL
The syringe of specification, 4 road liquid outlets of external offer, the nutrient solution entrance being respectively connecting in two media to cell culture modules 42, two
Group syringe is respectively independently driven by a high-resolution linear electric motors, and perfusion rate is accurately adjustable, meets various cell culture fields
Scape requirement.
Media to cell culture modules 42 is used to cultivate cell, and it needs stable environment temperature, therefore in media to cell culture modules 42
In be provided with temperature control component 421 and temperature sensor 422, temperature control component 421 is that semiconductor heats cooling piece, temperature sensor 422
For RTD, and media to cell culture modules 42 is coated with insulation material 423, to reduce temperature control power consumption.Media to cell culture modules 42
It is processed into using microfluidic chip technology design, multi-chamber can be made into using PDMS or PVC material combination polyester web frame
Micro-fluidic chip, support the co-cultivation of suspension cell and attached cell.
Enrichment module 43 connects with the nutrient solution outlet in media to cell culture modules 42, and utilizes magnetic field caused by magnetic sheet 431
Fixed superparamagnetic nanoparticle-antibody coupling matter therein, the conjugate are carried out to the cell factor detected needed for nutrient solution
Lasting enrichment；Enrichment module 43 is connected to waste collection module 45 and analysis module 44 by three-way magnetic valve 46.Magnetic sheet 431
The end of the output shaft of linear electric motors 432 is fixed on, it is driven closer or far from enrichment module 43 by linear electric motors 432, with production
Give birth to or remove the magnetic field around enrichment module 43.Specially：Enrichment module 43 is connected with the entrance of three-way magnetic valve 46, and three are powered
The one outlet of magnet valve 46 is connected with waste collection module 45, and another outlet is connected with analysis module 44.In concentration stage, three
Three-way electromagnetic valve 46 is in off-position, and the nutrient solution come out from enrichment module 43 is directly entered waste collection module 45；It has been enriched with
Cheng Hou, make magnetic sheet 431 away from enrichment module 43, i.e. magnetic field around enrichment module 43, three-way magnetic valve by linear electric motors 43
Electric on 46, the nutrient solution come out from enrichment module 43 enters analysis module 44.
Analysis module 44 is detected using superparamagnetic immune chromatograph test strips to the rho factor in nutrient solution, works as protein
The binding site of the factor is combined with the specific antibodies in the detection line of analysis module 44, and superparamagnetic nanoparticle is also aggregated simultaneously
Brown is revealed as in detection line, i.e., when testing result is positive, detection line is in brown.Analysis module 44 and waste collection module
45 are connected, and after detection, the nutrient solution in analysis module 44 enters waste collection module 45；Waste collection module 45 is customization 2D storages
Liquid bag, porch are provided with check valve.
Observing unit 5 includes the first image-forming assembly, the second image-forming assembly and data acquisition unit, wherein media to cell culture modules
Observation window is left in 42 lower sections, for the first image-forming assembly to the cellular morphology in media to cell culture modules 42, increment and migration situation
It is observed.The top of media to cell culture modules 42 is provided with the first LED424 for providing illumination.First image-forming assembly shows speck by 10
Mirror 425, the CCD imaging plates 427 of screw mandrel focusing structure 426 and the first form；Wherein, 10 x Microscope Objectives 425 are placed on the first CCD
On imaging plate 427, screw mandrel focusing structure 426 realizes the focusing to 10 x Microscope Objectives 425, and screw mandrel focusing structure 426 is by high score
Resolution linear electric motors drive, and meet the focusing demand of cell observation.
The second image-forming assembly is provided with analysis module 44, the second image-forming assembly is used to enter the testing result of analysis module 44
Row is taken pictures, including：Tight shot 441, the 2nd LED442 and the 2nd CCD imaging plates 443.Two group analysis modules 44 share it is a set of into
As component is taken pictures to testing result, the later stage can judge relevant cell by comparing detection line color change degree in control group
The quantum of output of the factor.
Data acquisition unit integrates Ethernet data interface using FPGA this Spartan-6 of match spirit series as main control chip,
And to the first image-forming assembly, the image data acquiring interface of the second image-forming assembly；Data acquisition unit is according under control unit 3
The process instruction of hair, the IMAQ to each image-forming assembly is completed, image is completed using DSP Texas Instrument TMS320C64 series
Stored after data compression to FLASH；And ground control centre will be reached under view data by Ethernet interface.Idle period,
Data acquisition unit is in off-position, to reduce system power dissipation, improves the stability of a system.Ethernet interface is set using maturation
Meter, interface chip W5100 has been internally integrated 10/100Mbps ethernet controllers, simple to operate, and stability is high.Net mouth transformer
Model H1102NL.CCD imaging plate output signals in image-forming assembly are pal mode, and design uses Philip SAA7113
Video decoding chip is acquired to three road signals.
As shown in fig. 6, IMAQ is initiated by control unit 3 by RS422 interfaces, the data after collection are by FPGA
Sent after portion RAM bufferings to DSP and do compression processing.Dsp software uses JPEG compression algorithm, compression ratio 3:1, single-frame images
Compression time is about 20ms, is stored in after the data back after compression to FPGA together with additional information in outside FLASH, adds letter
Breath include the affiliated image-forming assembly numbering of image, image frame number, shooting time and screening-mode it is real-time/regularly etc..Work as load test
When data transfer allows, data down transmission instruction is initiated by control unit, FPGA passes through 100,000,000 after reading historical data from FLASH
Passed under Ethernet, EEPROM is used to record the current amount of images for having stored and having passed down and frame number in FLASH, each for FPGA
Inquired about after upper electricity.
Using single-chip microcomputer Texas Instrument MSP430F5 series as main control chip, integrated 1553B bus remote measurements connect control unit 3
Mouthful, for each electric machine assembly, temperature control component, electromagnetic valve component, focusing component and illumination group in cell culture and analytic unit 4
Part is controlled；Control unit 3 is controlled to the upper electricity of data acquisition unit ethernet communication board and power-off, and is passed through
RS422 interfaces are communicated with the FPGA of data acquisition unit, and control FPGA completes observation and takes pictures and passed under view data.
1553B interface protocol chips use BU-61580S3, transformer model PM-DB2725.1553B buses telemetry interface can pass through
The aircraft that load is carried by device relevant operational state parameter it is real-time under reach ground control centre, and receive when necessary
The control instruction noted from ground.
The major function of the software of control unit 3 be according to experiment flow to the syringe pump of two groups of experimental systems, temperature control component,
Magnetic valve, image-forming assembly etc. are driven control, and are responsible for upper electricity and the power-off control of data acquisition unit, and control FPGA is completed
The autonomous focusing of timing is taken pictures；In addition, control software in real time passes through the key status parameters of system operation under 1553B telemetry bus
Pass, while support reception 1553B bus commands, the work(such as completion is taken pictures, passed under Real-time Focusing, history image data Ethernet in real time
Energy.
Referring to accompanying drawing 5, control software, which is adopted, to be shown a C language, and Software for Design opens the house dog carried inside single-chip microcomputer
Circuit, and timing preserves system operation key parameter, program fleet occurs and after watchdog reset when working long hours, can pass through
The timely recovery system running status of state reconstruction, it is ensured that the robustness of system operation.Add further, since internal system is present
The high power device such as refrigeration heat piece and motor, it is each high-power according to being actually needed reasonable distribution system power dissipation during Software for Design
Device time-sharing work, it is ensured that system power dissipation improves the security of system operation without departing from design requirement.
Further, for cell cultivation chip provide isoperibol temperature control component 421 be semiconductor heat cooling piece, type
Number it is TES1-07102, design is powered using 7VDC, utilizes metal-oxide-semiconductor commutating circuit to realize heating and refrigerating state switching.Temperature
Sensor 422 is RTD Pt1000, accesses after bridge for measuring temperature 16 A/D modules that the analog signal exported is carried by single-chip microcomputer
Collection is converted to Temperature numerical, and the conducting controlled quentity controlled variable of metal-oxide-semiconductor is calculated for pid algorithm, and then realizes semiconductor heating refrigeration
The heating and cooling of piece, the pid algorithm after optimization can realize 0.5 DEG C of accuracy of temperature control.
The motor of syringe pump in equipment, the linear electric motors observed under the focusing motor and enrichment chip of module are same
Serial high-resolution linear electric motors, running parameter are essentially identical.Design uses identical drive circuit, driving chip model moral
The serial four phase step actuators of the DRV88 of state instrument.Magnetic valve and illumination LED are driven using metal-oxide-semiconductor, in real work
In upper electricity, power-off control are carried out according to default experiment process to magnetic valve and illumination LED by single-chip microcomputer.
To sum up, presently preferred embodiments of the present invention is these are only, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements made etc., the protection of the present invention should be included in
Within the scope of.
Claims (9)
1. a kind of full automatic cells culture and analysis load, it is characterised in that including：ECU (1), load carrier (2) and
Positioned at the internal control unit (3) of the load carrier (2) and cell culture and analytic unit (4)；
The ECU (1) is used to provide direct current to described control unit (3) and the cell culture and analytic unit (4)
Electricity；
The cell culture and analytic unit (4) irrigate culture, to the richness of the protide factor in nutrient solution for carrying out cell
Collection and on-line analysis；Including：Syringe pump (41), media to cell culture modules (42), enrichment module (43), analysis module (44) and
Waste collection module (45)；
The syringe pump (41) is used to nutrient solution being injected into the media to cell culture modules (42), and its injection speed is by the control
Unit (3) control processed；
The media to cell culture modules (42) is used to cultivate cell, by temperature control component (421) to the media to cell culture modules (42)
Temperature control is carried out, the temperature control component (421) is controlled by described control unit (3)；
The enrichment module (43) is used for the cell detected needed for the nutrient solution that is flowed into from the media to cell culture modules (42)
The factor carries out continuing enrichment；
The analysis module (44) is used to carry out the rho factor in the nutrient solution after the enrichment module (43) enrichment
Detection, after completing detection, nutrient solution is emitted into the waste collection module (45).
2. a kind of full automatic cells culture as claimed in claim 1 and analysis load, it is characterised in that：Also include observing unit
(5), the observing unit (5) includes：Data acquisition unit and the first image-forming assembly；First image-forming assembly passes through described thin
Born of the same parents cultivate observation port below module (42) and the cellular morphology in the media to cell culture modules (42), increment and migration situation are carried out
Observation；First image-forming assembly includes：First CCD imaging plates (427), the micro- thing on the first CCD imaging plates (427)
Mirror (425) and the focusing structure for being focused to the microcobjective (425)；The focusing structure (426) is by described
Control unit (3) control is independently focused；
The data acquisition unit is adopted under the control of described control unit (3) to the image of first image-forming assembly
Collection, and the image of collection is compressed and stored.
3. a kind of full automatic cells culture as claimed in claim 2 and analysis load, it is characterised in that：The observing unit
(5) also include being used for the second image-forming assembly for taking pictures to the testing result of the analysis module (44)；Second imaging
Component includes：2nd CCD imaging plates (443), the tight shot (441) on the 2nd CCD imaging plates (443)；
The data acquisition unit is adopted under the control of described control unit (3) to the image of second image-forming assembly
Collection, and the image of collection is compressed and stored.
4. a kind of full automatic cells culture as claimed in claim 3 and analysis load, it is characterised in that：The observing unit
(5) also include being used to provide first image-forming assembly the first LED (424) of illumination and for second image-forming assembly
2nd LED (442) of illumination is provided；First LED (424) and the 2nd LED (442) opening and closing are by described control unit control
System.
5. a kind of full automatic cells culture and analysis load as described in claim 1,2 or 3, it is characterised in that：The cell
It is that the micro-fluidic chip of multi-chamber made of polyester web frame is engaged using PDMS or PVC material to cultivate module (42).
6. a kind of full automatic cells culture and analysis load as described in claim 1,2 or 3, it is characterised in that：The enrichment
Module (43) utilizes magnetic field caused by magnetic sheet (431) to fix superparamagnetic nanoparticle-antibody coupling matter therein, passes through the coupling
Thing carries out continuing enrichment to the cell factor detected needed for nutrient solution；
The magnetic sheet (431) linear electric motors (432) drive under closer or far from enrichment module (43), to produce or remove enrichment
Magnetic field around module (43)；The linear electric motors (432) are controlled by described control unit (3).
7. a kind of full automatic cells culture as claimed in claim 6 and analysis load, it is characterised in that：The enrichment module
(43) waste collection module (45) and analysis mould are connected to by the three-way magnetic valve (46) controlled by described control unit (3)
Block (44)；In concentration stage, control unit (3) controls the three-way magnetic valve (46) to be in off-position, and controls described straight
Line motor (432) makes the magnetic sheet (431) magnetic field be produced around enrichment module (43), from enrichment close to enrichment module (43)
The nutrient solution that module (43) comes out is directly entered waste collection module (45)；After the completion of enrichment, control unit (3) control is described straight
Line motor (432) makes the magnetic sheet (431) remove away from enrichment module (43) and produce magnetic field around enrichment module (43), and make
The three-way magnetic valve (46) is in "on" position, and the nutrient solution come out from enrichment module (43) enters analysis module (44).
8. a kind of full automatic cells culture and analysis load as described in claim 1,2 or 3, it is characterised in that：The cell
Culture module (42) is coated with insulation material (423).
9. a kind of full automatic cells culture and analysis load as described in claim 1,2 or 3, it is characterised in that：It is described automatically controlled
Unit (1) includes：Lithium battery (11) and power-switching circuit plate (12)；The power-switching circuit plate (12) is using DC-DC electricity
Flat conversion chip and relay realize the autonomous switching between aircraft platforms power supply and standby lithium pond (11) power supply.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711033458.7A CN107674837B (en) | 2017-10-30 | 2017-10-30 | Full-automatic cell culture and analysis load |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711033458.7A CN107674837B (en) | 2017-10-30 | 2017-10-30 | Full-automatic cell culture and analysis load |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107674837A true CN107674837A (en) | 2018-02-09 |
| CN107674837B CN107674837B (en) | 2021-04-02 |
Family
ID=61142935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711033458.7A Active CN107674837B (en) | 2017-10-30 | 2017-10-30 | Full-automatic cell culture and analysis load |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107674837B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643509A (en) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | High-adaptability full-automatic cell culture system and method thereof |
| CN110643508A (en) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | Modular automatic cell culture system and method |
| CN111394247A (en) * | 2020-03-31 | 2020-07-10 | 哈尔滨工业大学 | Constant-temperature heating system for cell in-situ culture |
| CN111948968A (en) * | 2020-08-16 | 2020-11-17 | 天津智橙物联科技有限公司 | High-throughput micro-reaction cell culture flexible automatic control system and method |
| CN113388500A (en) * | 2021-06-01 | 2021-09-14 | 南京大学 | Cell culture monitoring system and method capable of being used under microgravity |
| CN113699042A (en) * | 2021-08-17 | 2021-11-26 | 华艾博越(上海)生物医疗科技有限公司 | Control system of cell culture box |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110207209A1 (en) * | 2010-02-17 | 2011-08-25 | Aaron Hammons | Culture systems, apparatus, and related methods and articles |
| KR20110131371A (en) * | 2010-05-31 | 2011-12-07 | 전자부품연구원 | Cell trace device for fluorescence inspecting |
| CN202925029U (en) * | 2012-11-29 | 2013-05-08 | 重庆医科大学附属永川医院 | Temperature control device for perfusion cell culturing chip |
| CN103543260A (en) * | 2013-07-30 | 2014-01-29 | 中国检验检疫科学研究院 | Method for detecting biomacromolecule based on magnetic separation-quantum dot immunofluorescence sensing and reagent preparation method |
| CN105602846A (en) * | 2016-02-01 | 2016-05-25 | 北京理工大学 | Cell culture device for miniaturized space experiments |
| CN107064520A (en) * | 2017-03-30 | 2017-08-18 | 北京理工大学 | The system and method for protein micromolecular enrichment detection based on micro-fluidic chip |
-
2017
- 2017-10-30 CN CN201711033458.7A patent/CN107674837B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110207209A1 (en) * | 2010-02-17 | 2011-08-25 | Aaron Hammons | Culture systems, apparatus, and related methods and articles |
| KR20110131371A (en) * | 2010-05-31 | 2011-12-07 | 전자부품연구원 | Cell trace device for fluorescence inspecting |
| CN202925029U (en) * | 2012-11-29 | 2013-05-08 | 重庆医科大学附属永川医院 | Temperature control device for perfusion cell culturing chip |
| CN103543260A (en) * | 2013-07-30 | 2014-01-29 | 中国检验检疫科学研究院 | Method for detecting biomacromolecule based on magnetic separation-quantum dot immunofluorescence sensing and reagent preparation method |
| CN105602846A (en) * | 2016-02-01 | 2016-05-25 | 北京理工大学 | Cell culture device for miniaturized space experiments |
| CN107064520A (en) * | 2017-03-30 | 2017-08-18 | 北京理工大学 | The system and method for protein micromolecular enrichment detection based on micro-fluidic chip |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643509A (en) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | High-adaptability full-automatic cell culture system and method thereof |
| CN110643508A (en) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | Modular automatic cell culture system and method |
| CN111394247A (en) * | 2020-03-31 | 2020-07-10 | 哈尔滨工业大学 | Constant-temperature heating system for cell in-situ culture |
| CN111948968A (en) * | 2020-08-16 | 2020-11-17 | 天津智橙物联科技有限公司 | High-throughput micro-reaction cell culture flexible automatic control system and method |
| CN113388500A (en) * | 2021-06-01 | 2021-09-14 | 南京大学 | Cell culture monitoring system and method capable of being used under microgravity |
| CN113388500B (en) * | 2021-06-01 | 2024-03-12 | 南京大学 | Cell culture monitoring system and method applicable to microgravity |
| CN113699042A (en) * | 2021-08-17 | 2021-11-26 | 华艾博越(上海)生物医疗科技有限公司 | Control system of cell culture box |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107674837B (en) | 2021-04-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107674837A (en) | A kind of full automatic cells culture and analysis load | |
| CN205861115U (en) | A kind of aquaculture water quality automatic monitoring system | |
| CN103543773A (en) | Microenvironment intelligent control system and microenvironment intelligent control method | |
| CN106331173A (en) | Portable breeding monitoring device control box | |
| CN101294841A (en) | Automatic detecting device and method for measuring tank | |
| CN102920458B (en) | Multifunctional open type respiratory metabolism testing device | |
| CN104267349A (en) | Automobile generator testing system | |
| CN203561906U (en) | Microenvironment intelligent control system | |
| CN209260098U (en) | Bioreactor, control system | |
| CN207895289U (en) | A kind of oil smoke monitoring device based on NB-IoT | |
| Gong et al. | Adaptive smart control method for electric vehicle wireless charging system | |
| CN202942114U (en) | Multifunctional open-type respiratory metabolism determining device | |
| CN210377186U (en) | Bulky concrete intelligence temperature control system | |
| CN202206719U (en) | Energy-saving equipment cabinet capable of displaying and adjusting temperature | |
| CN204515573U (en) | A kind of heating radiator thermal property detects water temperature, flow control system | |
| CN110129200A (en) | A kind of 3D stem cell emulation culture device | |
| CN209102473U (en) | A kind of sulfur hexafluoride sampling system | |
| CN209013419U (en) | Portable air-conditioning system energy efficiency monitor | |
| CN201374136Y (en) | Multifunctional teaching device | |
| CN108071477B (en) | A kind of ocean robot cooling water control system and method | |
| CN206556786U (en) | Temperature and electric quantity measuring and converting device | |
| CN205507766U (en) | Birds animal house air current state real -time simulation device based on CFD | |
| CN209485461U (en) | A kind of detection system of emission flux of gas | |
| CN109405907B (en) | Method and system for detecting gas emission flux | |
| CN208188299U (en) | A kind of new energy battery test system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |