CN109609369A - A kind of anaerobe culture systems with automatic control monitoring function - Google Patents
A kind of anaerobe culture systems with automatic control monitoring function Download PDFInfo
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Abstract
A kind of anaerobe culture systems with automatic control monitoring function, control and monitoring system carries out control monitoring to the indoor environment of breeding strain room, strain transfer room and Spawn incubation room respectively, and will control monitoring information data transmission to terminal via network；External power supply inside control and monitoring system is connected to power module by pressure stabilizing rectification module, and power module is connect with control module, and by control module to other module for power supply；Pressure stabilizing rectification module includes voltage-stablizer, rectifier and transformer；The input terminal of signal processing module is connected with temperature control unit, humidity controlling unit, air cleaning member and monitoring camera-shooting unit；The output end of signal processing module is connect with A/D conversion module, the output end link control module of A/D conversion module, and control module is also connect with power module, memory module, GPRS communication module and signal output module simultaneously.
Description
Technical field
It is especially anaerobe culture technique field the present invention relates to field of microbial culture technology, especially one
Kind has the anaerobe culture systems for automatically controlling monitoring function.
Background technique
A major class of the microorganism including bacterium, virus, fungi and some small-sized protists, microalga etc.
Biocenose, its individual is small, close with human relation, covers beneficial to harmful numerous kinds, relates generally to food, doctor
The numerous areas such as medicine, industrial or agricultural, environmental protection, sport need to detect it in microbial cultivation process, can use sample at this time
Detection system.
The analysis found that there are following several for the existing anaerobe culture systems with automatic control monitoring function
The obvious problem of point:
(1) entire culture process cannot be monitored, and microorganism growing environment and extraneous factor can not be carried out real-time
It observes and records, while also cannot achieve automation, cause cost of human resources height.
(2) temperature control system monitoring range is limited, cannot effectively regulate and control to the temperature in temperature control module, and uses
It is more inconvenient.
(3) in humidity control process, the temperature and the indoor temperature of Spawn incubation for supplementing water exist humidity control system
Certain temperature difference, which results in the growing environments of microculture to change after humidification each time, trains to microorganism
Feeding there are certain uncontrolled variables, it is likely that has a negative impact to cultivation results.
(4) air cleaning system needs to be manually operated in aeration clearing process, it is necessary to which meeting in scavenging period cannot take
Air with oxygen enters Spawn incubation room, and needs to be filled with nitrogen and mixed gas several times to meet the nothing of anaerobic bacteria growth
Oxygen environment, specific operation process must satisfy specification, very complicated, and efficiency is lower, secondly, experimenter is to Spawn incubation room
Air environment in the middle can not accurately obtain concrete condition, be merely able to according to anaerobic bacteria growth rhythm and experiment experience judgement ventilation
It is clarification time, inconvenient for use.
Summary of the invention
Therefore, in order to solve above-mentioned deficiency, the present invention provides a kind of micro- with the anaerobism for automatically controlling monitoring function herein
Biological culture system.
It is a kind of with the anaerobe culture systems for automatically controlling monitoring function, include that breeding strain room, strain connect
Kind room, Spawn incubation room, control and monitoring system and terminal；Control and monitoring system respectively to breeding strain room, strain transfer room and
The indoor environment of Spawn incubation room carries out control monitoring, and will control monitoring information data via wireless network or cable network and pass
It send to terminal；The inside of control and monitoring system is equipped with control module, A/D conversion module, signal processing module, power module, letter
Number output module, GPRS communication module and memory module；External power supply is connected to power module, power supply by pressure stabilizing rectification module
Module is connect with control module, and by control module to other module for power supply；Pressure stabilizing rectification module includes voltage-stablizer, rectifier
And transformer；The input terminal of signal processing module is connected with temperature control unit, humidity controlling unit, air cleaning member and takes the photograph
As monitoring unit；The output end of signal processing module is connect with A/D conversion module, and the output end of A/D conversion module connects control
Module, control module are also connect with power module, memory module, GPRS communication module and signal output module simultaneously.
It is provided by the invention that there are the anaerobe culture systems for automatically controlling monitoring function, it can be to entire culture stream
Journey is monitored, and has been carried out real-time observation to microorganism growing environment and extraneous factor and has been recorded, has realized Automatic Control；Tool
Body advantage embodies are as follows:
Advantage 1: power supply is connect by power module with control module, for powering to control module, by control module to each
A mechanism is controlled, and realizes the Full-automatic monitoring of entire microculture process.
Advantage 2:PC computer and database server data communication, product are based on GPRS network and Internet network group
At message transmission subsystem, can satisfy the wider demand of detection device distribution, easily realize remote networking, convenient for pair
Each detection device carries out comprehensive long-range monitoring.
The present invention provides temperature control unit A, can easily monitor in real time to the temperature in microorganism temperature control module,
Temperature in microorganism temperature control module can be regulated and controled；Using simple and easy.Specific advantage embodies are as follows:
Advantage 1: terminal module is connect with CPU processor signal, and CPU processor is connect with electric control module signal, electric-controlled
Molding block is connect with temperature control module signal, by the way that terminal module, CPU processor, electric control module and temperature control module is mutual
Connection can regulate and control the temperature in temperature control module by terminal module convenient for user.
Advantage 2: temperature control module and electric control module can set multiple, and temperature control module and electric control module one are a pair of
Answer, the quantity of temperature control module and electric control module can be it is multiple, can be simultaneously to a variety of micro- lifes using the temperature control unit
Temperature in object temperature control module is regulated and controled.
Advantage 3: equipment monitoring device and slave computer are electrically connected, by the equipment monitoring device of setting can to temperature control module and
All parts in electric control module are monitored, and guarantee that the temperature control unit being capable of normal operation.
The present invention provides humidity controlling unit B, supplement water can be heated to Spawn incubation room temperature automatically before humidification,
It keeps Spawn incubation room temperature to meet microorganism growth always to require, and high degree of automation, it is easy to use；It is specific excellent
Point embodies are as follows:
Advantage 1: heating device is made of heater strip, temperature controller for water heater and indicator light, temperature controller for water heater and heater strip and after
Electric appliance C is electrically connected.The supplement water of humidifier is heated by the heating device of setting, makes humidifier to Spawn incubation room
Interior humidification water temperature is consistent with Spawn incubation room temperature；It by mains supply buckling is required by the transformer of power module
Power supply is become direct current by low-tension supply, rectifier, and after filtering, unstable DC voltage is become stable again by voltage-stablizer
Direct voltage output be automatic control module power supply.
Advantage 2: the condenser type hygrometric sensor of detecting module detects the indoor humidity condition of Spawn incubation, by integrated temperature
Measurement sensor detects the indoor temperature conditions of Spawn incubation, and the analog signal measured is by filters filter and output amplification
Device signal amplification after, by A D converter convert analog signals into digital signal input CPU processor in, enter CPU processor
Relay A, relay B, relay C and relay are controlled respectively according to the control preset value of Spawn incubation room temperature and humidity
D works to start air-dry machine, humidifier, water supply pump and heating device.
The present invention provides air cleaning member C, and intelligent automation controls aeration clearing, is not necessarily to manual hand manipulation, ventilation
Purification process guarantees that the air without carrying oxygen enters Spawn incubation room, without being filled with nitrogen and mixed gas several times, makes bacterium
Kind culturing room remains the oxygen-free environment of anaerobic bacteria growth, and can monitor Spawn incubation chamber interior air environment matter in real time
Amount, makes experimenter grasp accurate information always, facilitates experimental implementation, and grid more reasonable benefit/risk.Specific advantage body
It is existing are as follows:
Advantage 1: the inside of air cleaning member is additionally provided with equipment monitoring device, heat exchanger, deoxygenation machine, main filter, preposition mistake
Filter, post-filter, vacuum pump, temperature controller, automatic airtight air-valve A and automatic airtight air-valve B with equipment monitoring device signal
Connection.Anaerobic box internal gas quality is detected by temperature sensor, humidity sensor and gas detecting instrument first, is acquired
Data by A D converter convert analog signals into digital signal respectively be passed to slave computer and CPU processor in, at CPU
Data processing unit in reason device institute control module is transferred to storage unit after handling acquisition air quality data and carries out
Recording and storage, the effect of slave computer be monitoring to the indoor air quality of Spawn incubation and collocation equipment monitoring device for
The monitoring processing of equipment in equipment group subsystem, this have the advantage that, control is separated with monitoring first, has shared control
The performance load pressure of molding block while keeping the control response of system quickly timely, preferably realizes real-time monitoring communication, and
And introduce equipment monitoring device and can accomplish monitoring for equipment in equipment group subsystem, it after failure can be timely
Positioning failure source.
Advantage 2: if the Spawn incubation chamber interior air quality of anaerobic box is default lower than quality needed for anaerobic bacteria culture
When value, CPU processor starts fore filter, main filter, deoxygenation machine and heat exchanger first, and automatic in air inlet
Closed air-valve A is opened, and is entered outside air in deoxygenation machine after the purification of fore filter and main filter and is removed air
In oxygen molecule, enter back into heat exchanger, the strain according to measured by temperature sensor of the temperature controller in heat exchanger
Deoxygenation air is heated to being pumped into Spawn incubation room after Spawn incubation room temperature by vacuum pump by culture room temperature, strain
Low quality air in culturing room is then pushed into ventilation cabin by new gas, finally from air outlet after post-filter filters
Discharge, aeration clearing ensure that in the process enters Spawn incubation room without the air for carrying oxygen, remains that anaerobic bacteria grows institute
The oxygen-free environment needed, and it is not necessarily to manual hand manipulation, facilitate experimentation.
Advantage 3: one end of host computer is connected with mobile terminal, has network server between host computer and mobile terminal,
It is associated that host computer and mobile terminal carry out data by Internet with network server.Pass through the network service of setting
Mobile terminal can be used the monitoring and control in air cleaning member every the permission of one layer of host computer, upper in device
The means such as machine operating system firewall ensure the intranet security of air cleaning member, according to compared to being directly accessed typically now
The mode of external the Internet is compared, and keeps built-in system network safer, mobile terminal only by network server with it is upper
Machine is communicated, and obtains and permission is monitored and controlled, and host computer can also pass through the MAC address authentication or DNS in software technology
The means such as agreement carry out the certification of mobile terminal terminal, make the acquisition for mobile terminal permission only with certification, security of system is more
Add reasonable benefit/risk.
Detailed description of the invention
Fig. 1 is the module frame chart that the present invention has the anaerobe culture systems for automatically controlling monitoring function；
Fig. 2 is the whole implementation structure chart that the present invention has the anaerobe culture systems for automatically controlling monitoring function；
Fig. 3 is temperature control unit A module map of the present invention；
Fig. 4 is humidity controlling unit B module diagram of the present invention；
Fig. 5 is the detecting module schematic diagram of humidity controlling unit B of the present invention；
Fig. 6 is the heating-device modules schematic diagram of humidity controlling unit B of the present invention；
Fig. 7 is the structural schematic diagram of the air purification unit of that present invention C；
Fig. 8 is the system module schematic diagram of the air purification unit of that present invention C；
Fig. 9 is the mobile terminal and host computer communication module schematic diagram of the air purification unit of that present invention C.
Serial number as shown in the figure: breeding strain room 1；Strain transfer room 2；Spawn incubation room 3；Control and monitoring system 4；Voltage-stablizer
201；Rectifier 202；Transformer 203；Control module 401；A/D conversion module 402；Signal processing module 403；Power module
404；Signal output module 405；Memory module 406；GPRS communication module 407；Terminal 5；Detection device 6；Temperature control unit
A；Humidity controlling unit B；Monitoring camera-shooting cells D；Ultraviolet radiator；Bacteria sampler 605；Message transmission subsystem 9；PC computer
801；Database server 802；Information terminal subsystem 8；CPU processor A1；Control module A101；Temperature control module A2；Electrically
Control module A3；Temperature sensor A4；Temperature control instrument A5；A/D converter A6；Heater A7；Transformer A8；Power controller
A9；Timing unit A10；Data processing unit A11；Data storage cell A12；Equipment monitoring device A13；Slave computer A14；First nothing
Line transmission module A15；Second wireless transport module A16；Zigbee coordinator A17；Host computer A18；Alarm device A19；Terminal mould
Block A20；Automatic control module B1；CPU processor B101；Relay AB102；Relay BB103；Relay CB104；Relay
DB105；Air-dry machine B106；Humidifier B107；Heating device B108；Heat retaining and exothermal silk B1081；Heater strip B1082；Water heater temperature
Control device B1083；Indicator light B1084；Detecting module B3；Condenser type hygrometric sensor B301；Integrated temperature measurement sensor B302；
Filter B303；Output amplifier B304；A D converter B305；Air inlet C1；CPU processor C2；Control module C21；Switch
Timing control unit C22；Data processing unit C23；Storage unit C24；Main filter C3；Deoxygenation machine C4；Heat exchanger C5；It changes
Gas cabin C6；Post-filter C7；Air outlet C8；Air cleaning control module C9；Equipment monitoring device C91；A D converter C92；Under
Position machine C93；Zigbee module AC94；Zigbee module BC95；Sensing subsystem C10；Temperature sensor C101；Humidity sensor
C102；Gas detecting instrument C103；Equipment group subsystem C11；Vacuum pump C111；Automatic airtight air-valve AC113；Automatic airtight air-valve
BC114；Temperature controller C112；Power entry module C12；Communication system C13；Zigbee coordinator C131；Interchanger C132；Host computer
C14；Network server C15；Mobile terminal C16；Fore filter C17.
Specific embodiment
Below in conjunction with attached drawing, the present invention is described in detail, and technical solution in the embodiment of the present invention carries out clear
Chu is fully described by, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts all
Other embodiments shall fall within the protection scope of the present invention.
The present invention provides a kind of anaerobe culture systems with automatic control monitoring function, packet by improving herein
Breeding strain room 1, strain transfer room 2, Spawn incubation room 3, control and monitoring system 4 and terminal 5 are included；Control and monitoring system 4 divides
The other indoor environment to breeding strain room 1, strain transfer room 2 and Spawn incubation room 3 carries out control monitoring, and via wireless network
Or cable network will control monitoring information data transmission to terminal 5.
The inside of control and monitoring system 4 is equipped with control module 401, A/D conversion module 402, signal processing module 403, electricity
Source module 404, signal output module 405, GPRS communication module 407 and memory module 406.
External power supply is connected to power module 404 by pressure stabilizing rectification module, and power module 404 and control module 401 connect
It connects, and by control module 401 to other module for power supply；Pressure stabilizing rectification module includes voltage-stablizer 201, rectifier 202 and transformation
Device 203.
The input terminal of signal processing module 403 is connected with temperature control unit A, humidity controlling unit B, air cleaning member
C and monitoring camera-shooting cells D；The output end of signal processing module 403 is connect with A/D conversion module 402, A/D conversion module 402
Output end link control module 401, control module 401 simultaneously also with power module 404, memory module 406, GPRS communication module
407 and signal output module 405 connect.
Signal output module 405 transmits subsystem via wireless network or cable network and information terminal subsystem 8 and information
9 connection of system；Message transmission subsystem 9 includes wireless network and cable network, wireless network include GPRS network and
Internet network；Information terminal subsystem 8 includes database server 802 and PC computer 801；PC computer 801 and number
According to 802 data communication of library server；Control and monitoring system 4 and information terminal subsystem 8 pass through the realization number of message transmission subsystem 9
According to mutual transmission.
Power supply is connect by power module 404 with control module 401, for powering to control module 401, by controlling mould
Block 401 controls each mechanism, realizes the Full-automatic monitoring of entire microculture process, and control module 401 is realized
Line monitoring realizes that PC computer 801 and 802 data communication of database server, product are based on GPRS network and Internet
Network forms message transmission subsystem 9, can satisfy the wider demand of 6 distribution of detection device, easily realizes remote networking,
Convenient for carrying out comprehensive long-range monitoring to each detection device 6.
Operating method with the anaerobe culture systems for automatically controlling monitoring function: staff is in breeding strain
Microorganisms strain in room 1 transfers strain transfer room 2 after breeding strain, supermature strain is seeded in culture medium
On, finally the culture medium with strain is moved into Spawn incubation room 3 and carries out microculture, microorganism breeding strain room 1,
When in strain transfer room 2, Spawn incubation room 3, the real-time detection of examined equipment 6 passes through temperature control unit A, humidity control
Unit B processed, air cleaning member C, monitoring camera-shooting cells D in raising chamber 1, strain transfer room 2, Spawn incubation room 3 temperature,
Humidity, air quality and real-time imaging are measured in real time, and feed back to control module 401, and are carried out by memory module 406
Data storage, and staff can will control information by PC computer 801 and be sent to control by message transmission subsystem 9
Module 401, and control information is received by the GPRS communication module 407 of control module 401, the bacterial number in environment is supervised
It surveys, when bacterium is more in environment, ultraviolet radiator can be opened and carry out sterilization processing.
Temperature control unit A includes CPU processor A1, temperature control module A2 and electric control module A3, in CPU processor A1
Portion is equipped with control module A101, and control module A101 includes timing unit A10, data processing unit A11 and data storage cell
Temperature sensor A4 and heater A7 are respectively equipped with inside A12, temperature control module A2, temperature control module A2 neighbour side is equipped with electric-controlled molding
Block A3, electric control module A3 are made of temperature control instrument A5, A/D converter A6, transformer A8 and power controller A9, temperature control mould
The bottom block A2 is equipped with equipment monitoring device A13, and equipment monitoring device A13 neighbour side is equipped with slave computer A14, and slave computer A14 is connected to first
Wireless transport module A15, the first side wireless transport module A15 are equipped with terminal module A20, and terminal module A20 is assisted by Zigbee
Device A17 and host computer A18 is adjusted to constitute, Zigbee coordinator A17 is connected to the second wireless transport module A16；And temperature sensor
A4 and temperature control instrument A5 is electrically connected, and temperature control instrument A5 and A/D converter A6 are electrically connected, A/D converter A6 and CPU processor
A1 is electrically connected, and CPU processor A1 and the first wireless transport module 16 are electrically connected, the first wireless transport module 16 and Zigbee
Coordinator A17 is electrically connected, and Zigbee coordinator A17 electrically connects with host computer A18 and the first wireless transport module A15 respectively
It connects, the first wireless transport module A15 and slave computer A14 are electrically connected, and slave computer A14 is connect with power controller A9 signal, function
Rate controller A9 is connect with transformer A8 signal, and voltage device 8 and heater A7 are electrically connected, and temperature control module A2 and electrical control
Modules A 3 is electrically connected with equipment monitoring device A13.
External power supply is connected to power module 404 by pressure stabilizing rectification module, and power module 404 and control module 401 connect
It connects, and by control module 401 to other module for power supply；Pressure stabilizing rectification module includes voltage-stablizer 201, rectifier 202 and transformation
Device 203.
Terminal module A20 is connect with CPU processor A1 signal, and CPU processor A1 is connect with electric control module A3 signal,
Electric control module A3 is connect with temperature control module A2 signal, by by terminal module A20, CPU processor A1, electric control module
A3 and temperature control module A2 are connected with each other, can convenient for user by terminal module A20 come to the temperature in temperature control module A2 into
Row regulation.
Temperature control module A2 and electric control module A3 can set multiple, and temperature control module A2 and electric control module A3 mono- is a pair of
It answers, since the quantity of temperature control module A2 and electric control module A3 can be to be multiple, so can be same using temperature control unit A
When the temperature in multiple-microorganism temperature control module is regulated and controled.
Equipment monitoring device A13 and slave computer A14 is electrically connected, can be to temperature control mould by the equipment monitoring device A13 of setting
All parts in block A2 and electric control module A3 are monitored, and guarantee that temperature control unit A being capable of normal operation.
CPU processor A1 is connected to alarm device A19, and alarm device A19 and CPU processor A1 is electrically connected, and passes through setting
Alarm device A19 can the system when something goes wrong, sending is warned sound, and staff's on-call maintenance is reminded.
The operating method of temperature control unit A: temperature sensor A4 when use in temperature control module A2 can be to temperature control mould
Temperature in block A2 is monitored, the model PT100 of temperature sensor A4, and the data that will test are transferred to A/D and turn
Parallel operation A6, the analog signal after A/D converter A6 become digital signal, these signals can be transferred to CPU processor A1
Interior, the data after data processing unit A11 calculation processing are stored in data storage cell A12, data processing unit
A11 is the single-chip microcontroller of MCS-51, and data storage cell A12 is stored using DAS data storage method, while these signals pass through
It crosses the second wireless transport module A16 and is transferred to terminal module A20, user can be counted accordingly on terminal module A20
It is believed that breath, then judges according to data information, when needing to regulate and control the temperature in temperature control module A2, can be used eventually
Host computer A18 on end module A20 issues instruction, and instruction passes to slave computer A14 by the first wireless transport module A15, under
Position machine A14 issues power controller A9 according to instruction and instructs, then power controller A9 passes through change according to instruction regulation power
Depressor A8 adjusts the voltage in circuit, and to enable, heater A7 heats up or cooling, the first wireless transport module A15 and second are passed
Defeated module 16 be by Signal shock on the channel of microwave, launched by antenna, monitoring center pass through antenna receive
Microwave signal, then demodulates original signal by microwave receiver again, and timing unit A10 is a delay circuit, when making
When the output of energy end is effective, timer starts timing, and current value is incremented by, and when current value is greater than or equal to preset value, output state stops
Only, when enable end input is invalid, current value is remained unchanged, and when enable end is again switched on effective, passs on the basis of former memory value
Increase timing.
Humidity controlling unit B includes automatic control module B1 and detecting module B3, and automatic control module B1 includes CPU processing
Device B101, relay AB102, relay BB103, relay C105, relay D104, air-dry machine B106, humidifier B107, add
Thermal B108 and water supply pump 109, relay AB102, relay BB103, relay C105 and relay D104 are and at CPU
It manages device B101 to be electrically connected, air-dry machine B106 and relay AB102 are electrically connected, and humidifier B107 and relay BB103 are electrical
Connection, water supply pump 109 and relay D104 are electrically connected, and heating device B108 and relay C105 are electrically connected, and water supply pump
109 are set to the middle position of humidifier B107 and heating device B108, humidifier B107 and heating device B108 with water supply
109 connection of pump；
Detecting module B3 includes condenser type hygrometric sensor B301, integrated temperature measurement sensor B302, filter B303, output
Amplifier B304 and A D converter B305, condenser type hygrometric sensor B301 and integrated sensor for measuring temperature B302 with filter
The connection of wave device B303 signal, filter B303 and A D converter B305 connect with output amplifier B304 signal, and A D turn
Parallel operation B305 is connect with CPU processor B101 signal.
Heating device B108 is made of heater strip B1082, temperature controller for water heater B1083 and indicator light B1084, water heater temperature
It controls device B1083 and heater strip B1082 and relay C105 is electrically connected.By the heating device B108 of setting to humidifier B107
Supplement water heated, keep humidifier B107 humidification water temperature indoor to Spawn incubation consistent with Spawn incubation room temperature.
The condenser type hygrometric sensor B301 of detecting module B3 detects the indoor humidity condition of Spawn incubation, by integrated temperature
Measurement sensor B302 detect the indoor temperature conditions of Spawn incubation, the analog signal measured filtered through wave filter B303 and
Output amplifier B304 signal amplification after, by A D converter B305 convert analog signals into digital signal input CPU processing
In device B101, enters CPU processor B101 according to the control preset value of Spawn incubation room temperature and humidity and control relay respectively
AB102, relay BB103, relay C105 and relay D104 start air-dry machine B106, humidifier B107, water supply pump 109
It works with heating device B108.
CPU processor B101 is specially STC89C52 high-performance CMOS 8-bit microprocessor, integrated temperature measurement sensor
B302 is specially DS18B20 temperature transducer, the number of integrated temperature measurement sensor B302 and condenser type hygrometric sensor B301
Directly it is connected with the end P2.5 of CPU processor B101 according to interface, reconnecting the pull-up resistor that resistance value is 10K can be used.
STC89C52RC is 8 low-power consumption, high-performance CMOS micro- CPU processors, and there is 8K byte system may be programmed flash storage,
It is fine to possess dexterous 8 bit CPUs and in-system programmable components Flash, control response.
Indicator light B1084 is connect with temperature controller for water heater B1083 signal, shows heating by the indicator light B1084 of setting
The state of device B108.Heating device B108 further includes heat retaining and exothermal silk B1081, Heat preservation silk 1081 and temperature controller for water heater
B1083 is electrically connected；It can be kept the temperature by the heat retaining and exothermal silk B1081 of setting, reduce the use of main heating wire 1082, be saved
Save energy consumption.
Air cleaning member C includes air cleaning control module C9, communication system C13, host computer C14 and Power entry module
C12, air cleaning control module C9 and host computer C14 pass through communication system C13 mutual data transmission；Air cleaning control module
C9 by CPU processor C2, A D converter C92, slave computer C93, Zigbee module AC94 and Zigbee module BC95 constitute,
Zigbee module AC94 is installed in slave computer C93, and Zigbee module BC95 is installed in host computer C14；CPU processor C2's
Equipped with control module C21, the inside of control module C21 includes switch timing control unit C22, data processing unit for inside
C23 and storage unit C24.
Air cleaning control module C9 further includes having sensing subsystem C10 and equipment group subsystem C11, sensing subsystem 9
It is made of temperature sensor C101, humidity sensor C102 and gas detecting instrument C103, equipment group subsystem C11 is by heat exchanger
It is C5, deoxygenation machine C4, main filter C3, fore filter C17, post-filter C7, vacuum pump C111, temperature controller C112, automatic
Closed air-valve AC113 and automatic airtight air-valve BC114 is constituted, and one end of fore filter C17 is equipped with air inlet C1, automatic airtight
Air-valve AC113 is installed on inside air inlet C1, and one end of post-filter C7 is equipped with air outlet C8, automatic airtight air-valve BC114
It is installed on the inside of post-filter C7, and the opposite side of air outlet C8 is equipped with ventilation cabin C6.
Communication system C13 includes Zigbee coordinator C131 and interchanger C132.
Power entry module C12 and air cleaning control module C9 is electrically connected；Temperature sensor C101, humidity sensor C102
Connect with A D converter C92 signal with gas detecting instrument C103, and A D converter C92 output end respectively with CPU processor
The signal input part of C2 is connected with the equal signal of the signal input part of slave computer C93, heat exchanger C5, deoxygenation machine C4, main filter
C3, fore filter C17, post-filter C7, vacuum pump C111, temperature controller C112, automatic airtight air-valve AC113 and automatic close
Air-valve BC114 is closed to connect with the control signal output of CPU processor C2；Host computer C14 and slave computer C93 pass through Zigbee
Coordinator C131 carries out wireless data association, host computer C14 and CPU processor C2 and carries out data correlation by interchanger C132.
The inside of air cleaning control module C9 is additionally provided with equipment monitoring device C91, heat exchanger C5, deoxygenation machine C4, main mistake
Filter C3, fore filter C17, post-filter C7, vacuum pump C111, temperature controller C112, automatic airtight air-valve AC113 and from
Closed air-valve BC114 is moved to connect with equipment monitoring device C91 signal.By the air cleaning control module C9 of setting, first by
Temperature sensor C101, humidity sensor C102 and gas detecting instrument C103 detect anaerobic box internal gas quality, adopt
The data of collection by A D converter C92 convert analog signals into digital signal and be passed to slave computer C93 and CPU processor respectively
In C2, after the data processing unit C23 on CPU processor C2 institute control module C21 handles acquisition air quality data
It is transferred to storage unit C24 and carries out recording and storage, the effect of slave computer C93 is to the indoor air quality of Spawn incubation
Monitoring and collocation equipment monitoring device C91 handle the monitoring of equipment in equipment group subsystem C11, and the benefit done so exists
In control is separated with monitoring first, the performance load pressure of control module C21 has been shared, has kept the control response of system quick
While timely, real-time monitoring communication is preferably realized, and introduce equipment monitoring device C91 to accomplish for equipment group
The monitoring of equipment in system C11, after failure can timely positioning failure source.
When the preset value of quality needed for if the Spawn incubation chamber interior air quality of anaerobic box is lower than anaerobic bacteria culture,
CPU processor C2 starts fore filter C17, main filter C3, deoxygenation machine C4 and heat exchanger C5 first, and air inlet C1
Interior automatic airtight air-valve AC113 is opened, and enters outside air after the purification of fore filter C17 and main filter C3
The oxygen molecule in air is removed in deoxygenation machine C4, is entered back into heat exchanger C5, the temperature controller C112 in heat exchanger C5
Lead to after deoxygenation air is heated to Spawn incubation room temperature according to Spawn incubation room temperature measured by temperature sensor C101
It crosses vacuum pump C111 to be pumped into Spawn incubation room, the low quality air in Spawn incubation room is then pushed into ventilation cabin C6 by new gas
In the middle, it is finally discharged, ensure that during aeration clearing without carrying oxygen from air outlet C8 after post-filter C7 filtering
Air enter Spawn incubation room, remain anaerobic bacteria grow required for oxygen-free environment, and be not necessarily to manual hand manipulation,
Facilitate experimentation.
Control module C21 is also equipped with switch timing control unit C22.Pass through the switch timing control unit C22 of setting
Can start by set date air cleaning member C, multiple control modes combine, can more adapt to kinds of experiments scene.
One end of host computer C14 is connected with mobile terminal C16, has network clothes between host computer C14 and mobile terminal C16
It is associated that business device C15, host computer C14 and mobile terminal C16 carry out data by Internet with network server C15.It is logical
The network server C15 for crossing setting, makes mobile terminal C16 for the monitoring and control in air cleaning control module C9 every one
The permission of layer host computer C14 can be used the means such as host computer C14 operating system firewall and ensure air cleaning control module C9
Intranet security, compared according to compared to the mode for being directly accessed external the Internet typically now, make built-in system network more
Add safety, mobile terminal C16 is communicated only by network server C15 with host computer C14, and the power of monitoring and control is obtained
Limit, and host computer C14 can also carry out mobile terminal C16 by means such as MAC address authentication in software technology or DNS Protocol
The certification of terminal makes that only there is the mobile terminal C16 of certification to obtain permission, security of system more reasonable benefit/risk.
Vacuum pump C111 and temperature controller C112 is installed on the inside of heat exchanger C5, temperature sensor C101, humidity sensor
Device C102 and gas detecting instrument C103 are mounted on inside anaerobic bacteria culture case.Pass through the temperature sensor C101 of the setting, humidity
Sensor C102 and gas detecting instrument C103 carries out the air quality monitoring in incubator.
The operating method of air cleaning member C: air cleaning member C is tested suitable for anaerobic bacteria culture, including air is net
Change control module C9, communication system C13, host computer C14 and Power entry module C12, air cleaning control module C9, first by temperature
Sensor C101, humidity sensor C102 and gas detecting instrument C103 detect anaerobic box internal gas quality, acquisition
Data by A D converter C92 convert analog signals into digital signal respectively be passed to slave computer C93 and CPU processor C2 in,
Data processing unit C23 on CPU processor C2 institute control module C21 is transferred to after handling acquisition air quality data
Storage unit C24 carry out recording and storage, the effect of slave computer C93 be the monitoring to the indoor air quality of Spawn incubation with
The equipment monitoring device C91 that arranges in pairs or groups handles the monitoring of equipment in equipment group subsystem C11.Control is separated with monitoring, is shared
The performance load pressure of control module C21 while keeping the control response of system quickly timely preferably realizes real-time monitoring
It communicates, and introduce equipment monitoring device C91 to accomplish the monitoring of equipment in equipment group subsystem C11 occurring
It can timely positioning failure source after failure；If the Spawn incubation chamber interior air quality of anaerobic box is lower than anaerobic bacteria culture institute
When needing the preset value of quality, CPU processor C2 starts fore filter C17, main filter C3, deoxygenation machine C4 and heat exchange first
Device C5, and the automatic airtight air-valve AC113 in air inlet C1 is opened, make outside air by fore filter C17 and main mistake
Enter the oxygen molecule removed in air in deoxygenation machine C4 after the purification of filter C3, enters back into heat exchanger C5, heat exchange
Deoxygenation air is heated to by temperature controller C112 Spawn incubation room temperature according to measured by temperature sensor C101 in device C5
It is pumped into Spawn incubation room after Spawn incubation room temperature by vacuum pump C111, the low quality air in Spawn incubation room is then
It is pushed into ventilation cabin C6, is finally discharged after post-filter C7 filtering from air outlet C8, aeration clearing process by new gas
In ensure that without carry oxygen air enter Spawn incubation room, remain anaerobic bacteria grow required for oxygen-free environment, and
And it is not necessarily to manual hand manipulation, facilitate experimentation.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (8)
1. it is a kind of with the anaerobe culture systems for automatically controlling monitoring function, it include that breeding strain room (1), strain connect
Kind room (2), Spawn incubation room (3), control and monitoring system (4) and terminal (5)；It is characterized by: the control and monitoring system (4)
Control monitoring carried out to the indoor environment of breeding strain room (1), strain transfer room (2) and Spawn incubation room (3) respectively, and via
Wireless network or cable network will control monitoring information data transmission to terminal (5)；
The inside of the control and monitoring system (4) is equipped with control module (401), A/D conversion module (402), signal processing module
(403), power module (404), signal output module (405), GPRS communication module (407) and memory module (406)；External electrical
Source is connected to power module (404) by pressure stabilizing rectification module, and power module (404) is connect with control module (401), and is passed through
Control module (401) is to other module for power supply；The pressure stabilizing rectification module includes voltage-stablizer (201), rectifier (202) and transformation
Device (203)；It is net that the input terminal of signal processing module (403) is connected with temperature control unit (A), humidity controlling unit (B), air
Change unit (C) and monitoring camera-shooting unit (D)；The output end of signal processing module (403) is connect with A/D conversion module (402), A/
The output end link control module (401) of D conversion module (402), control module (401) simultaneously also with power module (404), deposit
Store up module (406), GPRS communication module (407) and signal output module (405) connection.
2. according to claim 1 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
Signal output module (405) is via wireless network or cable network and information terminal subsystem (8) and message transmission subsystem (9)
Connection；Message transmission subsystem (9) includes wireless network and cable network, wireless network include GPRS network and
Internet network；Information terminal subsystem (8) includes database server (802) and PC computer (801)；PC computer
(801) with database server (802) data communication；Control and monitoring system (4) and information terminal subsystem (8) are passed by information
Defeated subsystem (9) realizes that data are transmitted mutually.
3. according to claim 1 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
Temperature control unit (A) includes CPU processor (A1), temperature control module (A2) and electric control module (A3)；CPU processor (A1)
It is connect with electric control module (A3) signal, electric control module (A3) is connect with temperature control module (A2) signal；Temperature control module
(A2) and electric control module (A3) can set multiple, and temperature control module (A2) and electric control module (A3) are corresponded；
It is equipped with control module (A101) inside CPU processor (A1), control module (A101) includes timing unit (A10), data
Processing unit (A11) and data storage cell (A12)；Temperature sensor (A4) and heating are respectively equipped with inside temperature control module (A2)
Device (A7)；Temperature sensor (A4) and heater (A7) are separately mounted to breeding strain room (1), strain transfer room (2), strain training
It is internal to support room (3)；Temperature control instrument (A5), A/D converter (A6), transformer (A8) are respectively equipped with inside electric control module (A3)
With power controller (A9)；
Temperature sensor (A4) and temperature control instrument (A5) are electrically connected, and temperature control instrument (A5) and A/D converter (A6) are electrically connected,
A/D converter (A6) and CPU processor (A1) are electrically connected, and power controller (A9) is connect with transformer (A8) signal, voltage
Device (A8) and heater (A7) are electrically connected；Temperature control module (A2) and electric control module (A3) are connected to equipment monitoring device
(A13), equipment monitoring device (A13) is connected to slave computer (A14), slave computer (A14) and power controller (A9) feedback link；Under
Position machine (A14) is also attached to the first wireless transport module (A15), and the first wireless transport module (A15) is connected to terminal module
(A20)；Terminal module (A20) is made of Zigbee coordinator (A17) and host computer (A18)；CPU processor (A1) and the first nothing
Line transmission module (A16) is electrically connected, and the first wireless transport module (A16) and Zigbee coordinator (A17) are electrically connected,
Zigbee coordinator (A17) is electrically connected with host computer (A18) and the first wireless transport module (A15) respectively.
4. according to claim 3 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
It is characterized by: terminal module (A20) is connect with CPU processor (A1) signal, CPU processor (A1) is also electrically connected at alarm
Device (A19) and signal processing module (403).
5. according to claim 1 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
Humidity controlling unit (B) includes automatic control module (B1) and detecting module (B3)；
Automatic control module (B1) includes CPU processor (B101), relay A(B102), relay B(B103), relay C
(B105), relay D(B104), air-dry machine (B106), humidifier (B107), heating device (B108) and water supply pump (B109)；
Relay A-D is electrically connected with CPU processor (B101) respectively, air-dry machine (B106) and relay A(B102) it is electrically connected,
Humidifier (B107) and relay B(B103) be electrically connected, water supply pump (B109) and relay D(B104) be electrically connected, heating
Device (B108) and relay C(B105) it is electrically connected；Heating device (B108) is also connected to humidification via water supply pump (B109)
Device (B107)；
Detecting module (B3) includes condenser type hygrometric sensor (B301), integrated temperature measurement sensor (B302), filter
(B303), output amplifier (B304) and A D converter (B305)；Condenser type hygrometric sensor (B301) and integrated temperature measurement
Sensor (B302) is separately mounted to breeding strain room (1), strain transfer room (2), Spawn incubation room (3) inside；Condenser type is surveyed
Wet sensor (B301) and integrated sensor for measuring temperature (B302) are connect with filter (B303) signal, filter (B303)
Via A D converter (B305) be connected to output amplifier (B304), and A D converter (B305) and CPU processor (B101)
Signal connection.
6. according to claim 5 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
Heating device (B108) includes heat retaining and exothermal silk (B1081), heater strip (B1082), temperature controller for water heater (B1083) and instruction
Lamp (B1084)；Heat retaining and exothermal silk (B1081) and heater strip (B1082) are separately mounted to breeding strain room (1), strain transfer room
(2), Spawn incubation room (3) are internal；Heat preservation silk (B1081) and heater strip (B1082) are connected to temperature controller for water heater
(B1083), temperature controller for water heater (B1083) is connected to relay C(B105) and indicator light (B1084).
7. according to claim 1 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
Air cleaning member (C) includes air cleaning control module (C9), communication system (C13), host computer (C14) and Power entry module
(C12)；Air cleaning control module (C9) and host computer (C14) pass through communication system (C13) mutual data transmission；
Air cleaning control module (C9) include equipment monitoring device (C91), A D converter (C92), slave computer (C93),
Zigbee module A(C94), Zigbee module B(C95), CPU processor (C2), sensing subsystem (C10) and equipment group subsystem
(C11)；
Sensing subsystem (C10) be connected to equipment monitoring device (C91) and A D converter (C92), equipment monitoring device (C91)
With A D converter (C92) be all connected to slave computer (C93), slave computer (C93) is connected to communication via Zigbee module A(C94)
System (C13)；A D converter (C92) be connected to CPU processor (C2), CPU processor (C2) and host computer (C14) pass through logical
Letter system (C13) mutual data transmission；
Equipped with control module (C21), the inside of control module (C21) includes switch timing control for the inside of CPU processor (C2)
Unit (C22), data processing unit (C23) and storage unit (C24) processed；
Sensing subsystem (C10) includes temperature sensor (C101), humidity sensor (C102) and gas detecting instrument (C103)；
Temperature sensor (C101), humidity sensor (C102) and gas detecting instrument (C103) are connected to A D converter (C92)；
Equipment group subsystem (C11) includes main filter (C3), deoxygenation machine (C4), heat exchanger (C5), post-filter
(C7), fore filter (C17), vacuum pump (C111), temperature controller (C112), automatic airtight air-valve A(C113) and automatic airtight
Air-valve B(C114), and connect with the control signal output of CPU processor (C2)；One end of fore filter (C17) is equipped with
Air inlet (C1), automatic airtight air-valve A(C113) it is installed on air inlet (C1) inside, one end of post-filter (C7) is equipped with
Air port (C8), automatic airtight air-valve B(C114) it is installed on the inside of post-filter (C7), and the opposite side of air outlet (C8) is equipped with
It takes a breath cabin (C6)；Air outlet (C8) and air inlet (C1) are respectively arranged in breeding strain room (1), strain transfer room (2), strain training
It is internal to support room (3)；
Communication system (C13) includes Zigbee coordinator (C131) and interchanger (C132)；Host computer (C14) and slave computer
(C93) wireless data association is carried out by Zigbee coordinator (C131), host computer (C14) and CPU processor (C2) pass through friendship
Change planes (C132) carry out data correlation.
8. according to claim 7 have the anaerobe culture systems for automatically controlling monitoring function, it is characterised in that:
One end of host computer (C14) is connected with mobile terminal (C16), has network clothes between host computer (C14) and mobile terminal (C16)
It is engaged in device (C15), host computer (C14) and mobile terminal (C16) carry out data phase by Internet with network server (C15)
Association；Vacuum pump (C111) and temperature controller (C112) are installed on the inside of heat exchanger (C5)；Temperature sensor (C101), humidity
Sensor (C102) and gas detecting instrument (C103) are separately mounted to breeding strain room (1), strain transfer room (2), Spawn incubation
The inside of room (3).
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| CN201811582653.XA CN109609369A (en) | 2018-12-24 | 2018-12-24 | A kind of anaerobe culture systems with automatic control monitoring function |
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Application publication date: 20190412 |