CN107661569A - One kind is used for the oxidation resistant stem cell processed material micropin of skin - Google Patents
One kind is used for the oxidation resistant stem cell processed material micropin of skin Download PDFInfo
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- CN107661569A CN107661569A CN201610599344.8A CN201610599344A CN107661569A CN 107661569 A CN107661569 A CN 107661569A CN 201610599344 A CN201610599344 A CN 201610599344A CN 107661569 A CN107661569 A CN 107661569A
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- stem cell
- micropin
- processed material
- skin
- oxidation resistant
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/91—Injection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0023—Drug applicators using microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0046—Solid microneedles
Abstract
The invention discloses one kind to be used for the oxidation resistant stem cell processed material micropin of skin, described stem cell processed material micropin micropin and stem cell processed material made of metal material form, described stem cell processed material is stem cell media, and described stem cell is candidate stem cell, stem cell, NSC, amnion stem cell, dental pulp stem cell or palace film stem cell.The stem cell processed material micropin of the present invention, compared to vein or intramuscular injection, reduce the pain of user, operation is also simpler, conveniently, and will not cause environmental pollution；The stem cell used compared with prior art simultaneously or stem cell extract, cost is low, simple to operate, and the culture medium for cultivating stem cell is directly used, and avoids the waste of resource.
Description
Technical field
It is more particularly to a kind of micro- for the oxidation resistant stem cell processed material of skin the present invention relates to stem cell micropin field
Pin, for the calm melanin in skin of anti-oxidant and maintenance to skin.
Background technology
1999,《Science》HESC's achievement in research is chosen as first of ten big Progress ＆ New Products of the world then,
2000,《Time》Weekly be classified as 20 the end of the century world ten first of big technological achievement, and think embryonic stem cell and the mankind
Genome will most develop field with application prospect as the new century simultaneously.So far stem-cell research turns into biology in recent years
And one of most noticeable focus in medical domain.Stem cell (stem cells, SC) is that one kind has self-replacation energy
The multipotential cell of power (self-renewing), under certain condition, it can be divided into multiple functions cell.Stem cell
(Stem Cell) is a kind of inabundant differentiation, still jejune cell, has the potential work(for regenerating various histoorgans and human body
Energy, changes the countermeasure of human diseases, and medical field is referred to as " general-purpose cell ".
Stem-cell research has huge for beauty and health care with a variety of disease new treatments, maintenance and promotion human health is developed
Application prospect, therefore stem cells technology and its application be considered as 21 century human health engineering.Stem cell is a kind of special
Different cell, its biological property are the abilities (self-renewal) for both having had self-renewing, have multinomial differentiation potential again
(multilineage differentiation).There are some researches show：Fat stem cell also has oxidation resistant effect.
Stem-cell therapy, it is to make the stem cell of oneself or other people by vein, intramuscular injection or other modes or by doing
Cell differentiation and the mature cell that comes reaches a certain tissue, replaced or the treatment technology of damaged tissue repair.
In existing stem-cell therapy approach, stem cell transplantation is mainly carried out by way of vein, intramuscular injection,
But in addition to injection pain, injection process also needs to professional and technical personnel's operation, and injection needle apparatus will also result in environment dirt
Dye.In addition, prior art is used to treat or the composition of beauty and health care, substantially use after culture obtained stem cell or
It is the active principle extracted from stem cell, and ignores in stem cell incubation, secretion when cell growth is bred
Curative effect acts on, that is, cultivated the culture medium of stem cell in itself, its concentrate or extract can be used for treatment or U.S. of disease
Hold health care.
The content of the invention
The present invention provides one kind and is used for the oxidation resistant stem cell processed material micropin of skin, during culture stem cell, obtains
To stem cell media in itself, concentrate or extract micropin is made, it is calm in skin for the anti-oxidant and maintenance to skin
The melanin of skin.
Technical scheme is as follows：
One kind is used for the oxidation resistant stem cell processed material micropin of skin, and described stem cell processed material micropin is by metal material
Manufactured micropin and stem cell processed material composition, described stem cell processed material is stem cell media, and described stem cell is
Candidate stem cell, stem cell, NSC, amnion stem cell, dental pulp stem cell or palace film stem cell.
Further preferred, described stem cell processed material is the concentrate or extract of stem cell media.
Further preferred, described micropin is the micropin of patch pin, coating micropin or packaging medicine.
It is further preferably, described micropin is surface is smooth, there is the dimpled small wire matrix of groove or surface on surface.
Further preferred, the metal material of described micropin is titanium, copper, aluminium, iron, nickel, tungsten, stainless steel, titanium alloy, aluminium
One kind of alloy, nickel alloy or copper alloy.
It is further preferably, the surface of described micropin covered with more than one layer of film, the film be dielectric film,
Metallic film or emtal alloy film.
Further preferred, described micropin is empty micropin or solid microneedles, and the shank of described micropin is cylinder
Or cone, the length of described micropin is 10-2000um, and the needle point radius of curvature or equivalent radius of curvature of described micropin are
lnm-800^im。
The invention also discloses the preparation of described stem cell processed material, comprise the following steps：
(1) stem cell separation and extraction
The isolated described stem cell from tissue；
(2) expansion of stem cells
Isolated described stem cell is cultivated in the medium, 37 DEG C, 5%CO2Under conditions of expand, through processing
Obtain described stem cell media.
Further preferred, the separation method that described step (1) stem cell separation is used is centrifugal process, collagenase digesting
Method, tissue block method or immunomagnetic beads method.
Further preferred, the culture medium that described step (2) expansion of stem cells is used is DMEM, α-MEM or serum-free
Culture medium.
Compared with prior art, beneficial effects of the present invention are as follows：
One kind of the first, present invention is used for the oxidation resistant stem cell processed material micropin of skin, prepared by stem cell processed material
Into micropin, compared to vein or intramuscular injection, reduce pain when user uses, also more simple and side during operation
Just, it can complete to use without professional and technical personnel, and environmental pollution will not be caused；
One kind of the 2nd, present invention is used for the oxidation resistant stem cell processed material micropin of skin, will cultivate the process of stem cell
In, obtained stem cell media in itself, concentrate or extract micropin is made, i.e., when being bred using stem cell growth, training
The active ingredient of base secretion is supported, for the calm melanin in skin of anti-oxidant and maintenance to skin, therefore, the present invention is relative
Stem cell or stem cell extract for treating or beauty and health care are utilized in prior art, has preparation cost low, simple to operate, will train
The culture medium for supporting stem cell directly uses, and avoids the waste of resource.
Certainly, any product for implementing the present invention it is not absolutely required to reach all the above advantage simultaneously.
Brief description of the drawings
Fig. 1 is a kind of microstructure schematic diagram for the oxidation resistant stem cell processed material micropin of skin of the present invention.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate this hair
It is bright, rather than limit protection scope of the present invention.Those skilled in the art change according to what the present invention made in actual applications
Enter and adjust, still fall within protection scope of the present invention.
Shown in Fig. 1 is a kind of microstructure signal for the oxidation resistant stem cell processed material micropin of skin of the present invention
Figure, the preparation of described stem cell processed material, comprises the following steps：
(1) stem cell separation and extraction
It is isolated described by the method for centrifugation, collagenase digesting, tissue block method or immunomagnetic beads from tissue
Stem cell；
(2) expansion of stem cells
Isolated described stem cell is cultivated in DMEM, α-MEM or serum free medium, 37 DEG C, 5%CO2
Under conditions of expand, obtain described stem cell media or stem cell supernatant through processing.
In order to illustrate that one kind of the present invention is used for the oxidation resistant stem cell processed material micropin of skin, have to skin
The anti-oxidant and effect of melanin of the maintenance calmness in skin.Generally, when the stem cell processed material for preparing micropin has to skin
Anti-oxidant and maintenance calmness is when the effect of the melanin of skin, on the micropin that is prepared with the stem cell processed material similarly possesses
State effect.Therefore, the present invention is by by obtained stem cell processed material, including stem cell media in itself, concentrate or extraction
Thing, antioxidation is determined applied to cell culture, so as to the effect of indirectly showing stem cell processed material micropin.The present invention
Micropin is made in stem cell processed material, for the anti-oxidant of skin, compared to traditional injection or smearing method, have efficiently,
The advantages that long action time, sustained release.
Embodiment 1：Fat stem cell culture medium is applied to have antioxidation during cell culture
By the fat stem cell culture medium after obtained recovery and new complete medium 1:1 mixing, is used for as experimental group
Mouse melanin tumor cell (B16) cell culture；Concurrently set and use the B16 cells of new complete medium culture as blank
Group.By using the TAC of two groups of B16 cells of green skies TAC kit measurement, for evaluating fat
It is horizontal that can stem cell media lift the intracellular polyphenoils of B16.
Population statistics data
Independent sample is examined and determine
Obtained data are examined and determine more than can show that experimental group is equal with the data variance of control group, Sig=0.006<
0.01, so as to prove experimental group compared with control group, fat stem cell culture medium can be obviously improved the intracellular polyphenoils of B16
It is horizontal.
Embodiment 2：Concentrated-lyophilized obtained protein extract of stem cell media, which is used to have during cell culture, to be resisted
Oxidation
The stem cell media of recovery is carried out concentrating-frozen dried, obtains the protein in stem cell media.It will obtain
The protein extract obtained is redissolved in complete medium, and it is experimental group to prepare two concentration gradients of 4mg/ml and 2mg/ml respectively,
And set that to be not added with the complete medium of the protein extract be blank group.Three groups of culture mediums above are respectively used into dental pulp to do
Cell culture, using the TAC of green skies TAC kit measurement dental pulp stem cell, evaluate stem cell
It is horizontal that can the protein extract of culture medium lift polyphenoils in dental pulp stem cell.
Population statistics data
Oxidation resistance
The average value of group in homogeneity subset can be shown.
Use harmonic-mean sample size=4.000.
Statistical result shows that the stem cell media protein extract of 4mg/ml concentration still can be obviously improved culture
Dental pulp stem cell in oxidation resistance, and further surface, the stem cell protein extract that concentrated-frozen dried obtains
The activity of active principle in stem cell media can be retained.
The present invention culture medium for cultivating stem cell is returned, using stem cell media in itself, use stem cell
Culture medium concentrate or the active ingredient for extracting stem cell media, one kind is prepared and is used for the oxidation resistant stem cell of skin
Processed material micropin；And stem cell it is renewable be placed in an other culture medium continue cultivate or make stem cell as other purposes
With.Therefore, the present invention utilizes stem cell or stem cell extract for treating or beauty and health care relative to prior art, has cost
It is low, simple to operate, the culture medium for cultivating stem cell is directly used, the advantages that avoiding the waste of resource.
Present invention disclosed above preferred embodiment is only intended to help and illustrates the present invention.Preferred embodiment is not detailed
All details are described, it is only described embodiment also not limit the invention.Obviously, according to the content of this specification,
It can make many modifications and variations.This specification is chosen and specifically describes these embodiments, is to preferably explain the present invention
Principle and practical application so that skilled artisan can be best understood by and utilize the present invention.The present invention is only
Limited by claims and its four corner and equivalent.
Claims (10)
1. one kind is used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described stem cell processed material micropin
Micropin and stem cell processed material form made of metal material, and described stem cell processed material is stem cell media, described
Stem cell be candidate stem cell, stem cell, NSC, amnion stem cell, dental pulp stem cell or palace film stem cell.
2. according to claim 1 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is dry
Cell processed material is the concentrate or extract of stem cell media.
3. according to claim 1 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is micro-
Pin is the micropin of patch pin, coating micropin or packaging medicine.
4. according to claim 1 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is micro-
Pin is that surface is smooth, there is the dimpled small wire matrix of groove or surface on surface.
5. according to claim 1 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is micro-
The metal material of pin is one kind of titanium, copper, aluminium, iron, nickel, tungsten, stainless steel, titanium alloy, aluminium alloy, nickel alloy or copper alloy.
6. according to claim 1 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is micro-
Covered with more than one layer of film, described film is dielectric film, metallic film or emtal alloy film on the surface of pin.
7. according to claim 6 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is micro-
Pin is empty micropin or solid microneedles, and the shank of described micropin is cylinder or cone, and the length of described micropin is 10-
2000um, the needle point radius of curvature or equivalent radius of curvature of described micropin are lnm-800^im.
8. according to claim 1 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described is dry
The preparation of cell processed material, comprises the following steps：
(1) stem cell separation and extraction
The isolated described stem cell from tissue；
(2) expansion of stem cells
Isolated described stem cell is cultivated in the medium, 37 DEG C, 5%CO2Under conditions of expand, through processing obtain
Described stem cell media.
9. according to claim 8 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described step
Suddenly the separation method that the separation of (1) stem cell is used is centrifugal process, collagenase digestion, tissue block method or immunomagnetic beads method.
10. according to claim 8 be used for the oxidation resistant stem cell processed material micropin of skin, it is characterised in that described
The culture medium that step (2) expansion of stem cells is used is DMEM, α-MEM or serum free medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610599344.8A CN107661569A (en) | 2016-07-27 | 2016-07-27 | One kind is used for the oxidation resistant stem cell processed material micropin of skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610599344.8A CN107661569A (en) | 2016-07-27 | 2016-07-27 | One kind is used for the oxidation resistant stem cell processed material micropin of skin |
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| CN107661569A true CN107661569A (en) | 2018-02-06 |
Family
ID=61115054
Family Applications (1)
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| CN201610599344.8A Pending CN107661569A (en) | 2016-07-27 | 2016-07-27 | One kind is used for the oxidation resistant stem cell processed material micropin of skin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109394563A (en) * | 2018-11-06 | 2019-03-01 | 福建省海西细胞生物工程有限公司 | A kind of preparation method of stem cell media liposome and the application in skin care item |
| CN113322227A (en) * | 2021-04-29 | 2021-08-31 | 南京医科大学 | Testicle tissue in vitro culture method based on microneedle and application thereof |
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| JP2005154321A (en) * | 2003-11-25 | 2005-06-16 | Nano Device & System Research Inc | Microneedle for ascorbic acid administration |
| CN104288129A (en) * | 2014-09-30 | 2015-01-21 | 奥思达干细胞有限公司 | Stem cell micro-needle patch for resisting wrinkles and removing freckles and preparation method thereof |
| US20150299650A1 (en) * | 2014-04-18 | 2015-10-22 | Growgene Biotech Inc. | Use of stem cell conditioned medium to inhibit oxidation for anti-aging skin |
| CN104997708A (en) * | 2015-07-07 | 2015-10-28 | 河南中科干细胞基因工程有限公司 | Obtaining method of stem cell conditioned medium, and application of stem cell conditioned medium in skin ageing resistance |
| CN105025976A (en) * | 2013-01-21 | 2015-11-04 | Bukyoung药业有限公司 | Microneedle, mould for producing same, and production method for same |
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2016
- 2016-07-27 CN CN201610599344.8A patent/CN107661569A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005154321A (en) * | 2003-11-25 | 2005-06-16 | Nano Device & System Research Inc | Microneedle for ascorbic acid administration |
| CN105025976A (en) * | 2013-01-21 | 2015-11-04 | Bukyoung药业有限公司 | Microneedle, mould for producing same, and production method for same |
| US20150299650A1 (en) * | 2014-04-18 | 2015-10-22 | Growgene Biotech Inc. | Use of stem cell conditioned medium to inhibit oxidation for anti-aging skin |
| CN104288129A (en) * | 2014-09-30 | 2015-01-21 | 奥思达干细胞有限公司 | Stem cell micro-needle patch for resisting wrinkles and removing freckles and preparation method thereof |
| CN104997708A (en) * | 2015-07-07 | 2015-10-28 | 河南中科干细胞基因工程有限公司 | Obtaining method of stem cell conditioned medium, and application of stem cell conditioned medium in skin ageing resistance |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109394563A (en) * | 2018-11-06 | 2019-03-01 | 福建省海西细胞生物工程有限公司 | A kind of preparation method of stem cell media liposome and the application in skin care item |
| CN113322227A (en) * | 2021-04-29 | 2021-08-31 | 南京医科大学 | Testicle tissue in vitro culture method based on microneedle and application thereof |
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Application publication date: 20180206 |