CN107647931A - A kind of fish fixing device for being used to before brain tissue sample fix and implementation - Google Patents
A kind of fish fixing device for being used to before brain tissue sample fix and implementation Download PDFInfo
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- CN107647931A CN107647931A CN201711094762.2A CN201711094762A CN107647931A CN 107647931 A CN107647931 A CN 107647931A CN 201711094762 A CN201711094762 A CN 201711094762A CN 107647931 A CN107647931 A CN 107647931A
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- China
- Prior art keywords
- fish
- side wall
- fixed cover
- bottom plate
- fixing device
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
Abstract
A kind of fish fixing device for being used to before brain tissue sample fix and implementation, including the rectangle shell structure being made up of bottom plate, side wall and fixed cover, side wall is installed on four sides of bottom plate, fixed cover is installed at the top of bottom plate, the U-shaped dividing plate of slidably adjusting position is provided with the inside of shell structure；Fish belly portion operation window is opened up in a side wall wherein；The fish chest operation window of gap shape or window-like is opened up in fixed cover.Implementation is that fish is carried out into dipping anesthesia；Fish is lain on bottom plate；According to the U-shaped partition position of body top adjustment of fish, U-shaped dividing plate is fixed with side wall with buckle；According to the distance between the wide regulation fixed cover of the body of fish and side wall, fixed cover and side wall are fixed with screw；Fish chest operation window in fixed cover carries out experimental implementation to fish body thorax abdomen side；Experimental implementation is carried out to fish thorax abdomen bottom surface at fish belly portion operation window in the sidewall.The present invention can fix various sizes of fish body, convenient to operate, using simple.
Description
Technical field
The present invention relates to BIOLOGICAL CONTROL field, it is especially a kind of to fish thorax abdomen carry out experimental implementation when fixing device and
Implementation.
Background technology
Brain is higher nervous center, and brain kinesitherapy nerve core group can control the motion of animal, so commonly used in the world
Brain control technical controlling animal robot.Brain control technology is the key technology of animal robot BIOLOGICAL CONTROL, wherein brain tissue slice
It is the important content of brain control technology, extraction brain tissue sample is then premise prepared by brain tissue slice.
Fixation is to extract a kind of effective new method of good brain tissue sample before brain tissue sample, and its effect is better than biography
Fixation after the brain tissue sample of system.Fixation is to carry out heart filling with fixer under Animal Anesthesia state before brain tissue sample
Flow complete and brain tissue sample in good condition a kind of experimental method in the form of obtaining.Carrying out cardiac perfusion need to enter to fish thorax abdomen
Row dissection operation exposure heart, but fish body surface is smooth, is not fixed easily, and operating difficulties, time length is spent, influences experiment behaviour
Make, therefore a kind of need to meet the fish fixing device and implementation of fixation requirement of experiment before brain tissue sample.
Adjustable fish body fixer [J] education is studied with equipment, 2016 (9)：66-67 is by rectangle tank, one
Block L-shaped dividing plate, one piece of U-shaped dividing plate three parts composition, also use a buckle, a removable bolt, for observing fish in addition
Or probe into fish movement.
The U's of patent No. CN 201467895《A kind of fish fixing device of fish pretreatment machine》Including to fish head portion and fish
Body device for positioning and securing so that machinery the scaling of fish, cutting, remove internal organ, remove black film etc. during positioning effective to fish it is solid
It is fixed.
The A's of patent No. CN 104359706《One kind detection fish sampling fixing device and implementation》Including substrate, admittedly
Determine neck and fixed steel bar, the fish of sampling be fixed when food safety detection fish sample, be easy to smoothly complete scale,
Peeling and sampling operation, increase the convenience and security of sampling operation.
Patent No. CN 205143373U's《Domestic simple and easy fish auxiliary fixture
that kills》Disclose a kind of fish auxiliary clamp easy to use, the device using three connecting rods, spring, arc clamping plate come
Fixed fish, conveniently butchers.
In summary, although some existing fish fixing devices are used for the fixation and processing of fish now, fish chest and abdomen are directed to
Portion carries out dissection operation and is used for fixed fixing device before brain tissue sample and implementation there is not yet document report, so research
It is a kind of it is easy to operate, using simple, and it is necessary to be favorably improved the fish fixing device of experiment accuracy and implementation.
The content of the invention
Present invention aims at provide a kind of various sizes of fish body can be fixed, be easy to enter the thorax abdomen of fish
Dissection of going operates, implemented the fish fixing device for being used to before brain tissue sample fix and implementation of cardiac perfusion.
To achieve the above object, following technical scheme is employed：It is of the present invention a kind of for fixed before brain tissue sample
Fish fixing device, the main rectangle shell structure for including being made up of bottom plate, side wall and fixed cover, pacify on four sides of bottom plate
Side wall is filled, fixed cover is installed at the top of bottom plate, the U-shaped dividing plate of slidably adjusting position is provided with the inside of shell structure；
Fish belly portion operation window is opened up in one of side wall；The fish chest operation window of gap shape or window-like is opened up in fixed cover.
Further, some thread grooves are opened up along the top margin of side wall, corresponding to each thread groove opening position in fixed cover opens up spiral shell
Line mounting hole, screw is connected through mounting hole with thread groove links together fixed cover, side wall.
Further, the both ends raised section of the U-shaped dividing plate fits with adjacent side wall, and in the projection of U-shaped dividing plate
Buckle is installed in section, buckle is connected with side wall to be fixed.
Further, the surface of the bottom plate is coarse non slip surface.
A kind of fish for being used to before brain tissue sample fix of the present invention fixes implementation, comprises the following steps：
Step 1, fish is subjected to dipping anesthesia；
Step 2, postanesthetic fish is lain against on the bottom plate in fixing device；
Step 3, used according to the distance of fish belly portion operation window on the U-shaped dividing plate of body top adjustment of fish and side wall, position after determining
Buckle fixes U-shaped dividing plate with side wall；
Step 4, according to the distance between the wide regulation fixed cover of the body of fish and side wall, position determine after with screw by fixed cover with
Side wall is fixed, and pushes down fish body using fixed cover so as to complete the fixation to fish；
Step 5, the fish chest operation window in fixed cover carries out experimental implementation to fish body thorax abdomen side；
Step 6, experimental implementation is carried out to fish thorax abdomen bottom surface at fish belly portion operation window in the sidewall.
Compared with prior art, the invention has the advantages that：
1st, experiment behaviour is conveniently carried out respectively to the side of fish thorax abdomen and bottom surface positioned at 2 operation windows of side wall and fixed cover
Make；
2nd, it according to wide regulation the distance between the fixed cover and fish body of the body of fish and can be screwed, make fixing device highly
It is adjustable；It can be fixed according to the distance between the U-shaped dividing plate of body top adjustment of fish and side wall and with buckle, make fixing device width can
Adjust, can arbitrarily be adjusted according to fish body size in practical application.
Brief description of the drawings
Fig. 1 is the diagrammatic perspective view of apparatus of the present invention.
Fig. 2 is the internal structure schematic diagram of apparatus of the present invention.
Fig. 3 is the fixed cover figure of apparatus of the present invention.
Fig. 4 is the U-shaped dividing plate figure of apparatus of the present invention.
Drawing reference numeral：1- side walls, 2- bottom plates, 3- fish bellies portion operation window, 4-U types dividing plate, 5- buckles, 6- fixed covers, 7- spiral shells
Silk, 8- fish chest operation windows.
Embodiment
The present invention will be further described below in conjunction with the accompanying drawings：
As Figure 1-4, the invention mainly comprises the rectangle shell structure being made up of bottom plate 2, side wall 1 and fixed cover 6,
Side wall is installed on four sides of bottom plate, fixed cover is installed at the top of bottom plate, is provided with the inside of shell structure and slidably adjusts
The U-shaped dividing plate 4 of position；Fish belly portion operation window 3 is opened up in a side wall wherein；Gap shape or window-like are opened up in fixed cover
Fish chest operation window 8.Some thread grooves are opened up along the top margin of side wall, corresponding to each thread groove opening position in fixed cover opens up screw thread
Mounting hole, screw 7 is connected through mounting hole with thread groove links together fixed cover, side wall.The both ends of the U-shaped dividing plate are convex
Play section to fit with adjacent side wall, and buckle 5 is installed on the raised section of U-shaped dividing plate, buckle is connected with side wall to be fixed.
The surface of the bottom plate is coarse non slip surface, and coarse non slip surface can increase the frictional force between fish body and bottom plate, improve stability.
Implementation of the present invention, comprises the following steps：
1st, select a tail health carp, the high 11cm of body, the wide 6cm of body, the long 34.7cm of body, the body it is a length of from carp rhynchodaenm to
The distance of fish scale and tail fin intersection, carp is placed in progress dipping anesthesia in cloves phenol solution.
2nd, it is 50 × 20 × 5cm to keep flat the carp of anesthesia into volume3Fixing device in bottom plate on.
3rd, a length of 50cm of U-shaped dividing plate, shorter two side length are 2cm, according between the U-shaped dividing plate of body top adjustment and side wall of fish
Distance, and fixed with buckle.
4th, fixed cover area is 50 × 20cm2, fixed cover is covered, according between the wide regulation fixed cover of the body of fish and side wall
Distance is simultaneously screwed, so as to complete the fixation to testing carp.
5th, fish chest operation window ara is 8 × 6cm2, it is 6cm away from fixed cover drift angle distance, passes through fish chest operation window pair
The side of fish thorax abdomen carries out experimental implementation.
6th, operation window ara in fish belly portion is 8 × 1cm2, it is corresponding with fish chest operation window position, pass through fish belly portion operation window
Experimental implementation is carried out to the bottom surface of fish thorax abdomen.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the model of the present invention
Enclose and be defined, on the premise of design spirit of the present invention is not departed from, technical side of the those of ordinary skill in the art to the present invention
The various modifications and improvement that case is made, it all should fall into the protection domain of claims of the present invention determination.
Claims (5)
1. a kind of fish fixing device for being used to before brain tissue sample fix, main to include what is be made up of bottom plate, side wall and fixed cover
Rectangle shell structure, it is characterised in that：Side wall is installed on four sides of bottom plate, fixed cover is installed at the top of bottom plate, in shell
The inside of body structure is provided with the U-shaped dividing plate of slidably adjusting position；Fish belly portion operation window is opened up in a side wall wherein；Solid
Surely the fish chest operation window for opening up gap shape or window-like is covered.
A kind of 2. fish fixing device for being used to before brain tissue sample fix according to claim 1, it is characterised in that：Along side
The top margin of wall opens up some thread grooves, and corresponding to each thread groove opening position in fixed cover opens up threaded mounting hole, and screw passes through installation
Hole is connected with thread groove links together fixed cover, side wall.
A kind of 3. fish fixing device for being used to before brain tissue sample fix according to claim 1, it is characterised in that：It is described
The both ends raised section of U-shaped dividing plate is fitted with adjacent side wall, and buckle, buckle and side are installed on the raised section of U-shaped dividing plate
Wall, which is connected, to be fixed.
A kind of 4. fish fixing device for being used to before brain tissue sample fix according to claim 1, it is characterised in that：It is described
The surface of bottom plate is coarse non slip surface.
5. a kind of fish for being used to before brain tissue sample fix based on claim 1 fixes implementation, it is characterised in that described
Method comprises the following steps：
Step 1, fish is subjected to dipping anesthesia；
Step 2, postanesthetic fish is lain against on the bottom plate in fixing device；
Step 3, buckle is used after determining according to the distance of fish belly portion operation window on the U-shaped dividing plate of body top adjustment of fish and side wall, position
U-shaped dividing plate is fixed with side wall；
Step 4, according to the distance between the wide regulation fixed cover of the body of fish and side wall, position determine after with screw by fixed cover and side wall
It is fixed, fish body is pushed down using fixed cover so as to complete the fixation to fish；
Step 5, the fish chest operation window in fixed cover carries out experimental implementation to fish body thorax abdomen side；
Step 6, experimental implementation is carried out to fish thorax abdomen bottom surface at fish belly portion operation window in the sidewall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711094762.2A CN107647931B (en) | 2017-11-09 | 2017-11-09 | Fixed fish fixing device and implementation method before a kind of sample for brain tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711094762.2A CN107647931B (en) | 2017-11-09 | 2017-11-09 | Fixed fish fixing device and implementation method before a kind of sample for brain tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107647931A true CN107647931A (en) | 2018-02-02 |
| CN107647931B CN107647931B (en) | 2019-07-26 |
Family
ID=61120668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711094762.2A Active CN107647931B (en) | 2017-11-09 | 2017-11-09 | Fixed fish fixing device and implementation method before a kind of sample for brain tissue |
Country Status (1)
| Country | Link |
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| CN (1) | CN107647931B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87210906U (en) * | 1987-07-23 | 1988-04-27 | 廖信汤 | Combined fish sieve |
| DE102010032673A1 (en) * | 2010-03-13 | 2011-09-15 | Siegfried Beck | Axial, cylindrical pipe construction for use in fish cage, has ballast chambers that are stable and flexible in axial, cylindrical sectional spaces, where ballast chambers control complete cage only with corresponding air volume |
| CN203748465U (en) * | 2014-04-09 | 2014-08-06 | 中国水产科学研究院黑龙江水产研究所 | Anti-jumping fixing device for aquarium fish culture |
| CN104257439A (en) * | 2014-10-28 | 2015-01-07 | 沈阳医学院 | Enclosed animal experiment operation box |
| CN104568542A (en) * | 2014-12-30 | 2015-04-29 | 燕山大学 | Method for fixing fish brain tissue specimen through systemic heart perfusion |
| CN105309380A (en) * | 2014-08-04 | 2016-02-10 | 四川省鸿祥科技有限公司 | Conveniently-assembled stereoscopic turtle box |
| CN106473828A (en) * | 2015-09-02 | 2017-03-08 | 中国长江三峡集团公司中华鲟研究所 | A kind of mandarin sturgeon brain tissue sample sampling bench |
| CN206102774U (en) * | 2016-07-27 | 2017-04-19 | 贵州大学 | Fish is with muscle blood collection system |
| CN206151626U (en) * | 2016-08-18 | 2017-05-10 | 中国水产科学研究院黑龙江水产研究所 | Live fish inspection underwater operation platform |
-
2017
- 2017-11-09 CN CN201711094762.2A patent/CN107647931B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87210906U (en) * | 1987-07-23 | 1988-04-27 | 廖信汤 | Combined fish sieve |
| DE102010032673A1 (en) * | 2010-03-13 | 2011-09-15 | Siegfried Beck | Axial, cylindrical pipe construction for use in fish cage, has ballast chambers that are stable and flexible in axial, cylindrical sectional spaces, where ballast chambers control complete cage only with corresponding air volume |
| CN203748465U (en) * | 2014-04-09 | 2014-08-06 | 中国水产科学研究院黑龙江水产研究所 | Anti-jumping fixing device for aquarium fish culture |
| CN105309380A (en) * | 2014-08-04 | 2016-02-10 | 四川省鸿祥科技有限公司 | Conveniently-assembled stereoscopic turtle box |
| CN104257439A (en) * | 2014-10-28 | 2015-01-07 | 沈阳医学院 | Enclosed animal experiment operation box |
| CN104568542A (en) * | 2014-12-30 | 2015-04-29 | 燕山大学 | Method for fixing fish brain tissue specimen through systemic heart perfusion |
| CN106473828A (en) * | 2015-09-02 | 2017-03-08 | 中国长江三峡集团公司中华鲟研究所 | A kind of mandarin sturgeon brain tissue sample sampling bench |
| CN206102774U (en) * | 2016-07-27 | 2017-04-19 | 贵州大学 | Fish is with muscle blood collection system |
| CN206151626U (en) * | 2016-08-18 | 2017-05-10 | 中国水产科学研究院黑龙江水产研究所 | Live fish inspection underwater operation platform |
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| CN107647931B (en) | 2019-07-26 |
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