CN107647931B - Fixed fish fixing device and implementation method before a kind of sample for brain tissue - Google Patents
Fixed fish fixing device and implementation method before a kind of sample for brain tissue Download PDFInfo
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- CN107647931B CN107647931B CN201711094762.2A CN201711094762A CN107647931B CN 107647931 B CN107647931 B CN 107647931B CN 201711094762 A CN201711094762 A CN 201711094762A CN 107647931 B CN107647931 B CN 107647931B
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- fish
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- side wall
- bottom plate
- shaped partition
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Processing Of Meat And Fish (AREA)
Abstract
Fixed fish fixing device and implementation method before a kind of sample for brain tissue, including the rectangle shell structure being made of bottom plate, side wall and fixed lid, side wall is installed on four sides of bottom plate, the fixed lid of installation at the top of bottom plate is equipped with the U-shaped partition of slidably adjusting position in the inside of shell structure；Fish belly portion operation window is opened up on a side wall wherein；The fish chest operation window for opening up gap shape or window-like is covered fixing.Implementation method is that fish is carried out dipping anesthesia；Fish is lain on bottom plate；According to the U-shaped partition position of body top adjustment of fish, U-shaped partition is fixed with side wall with buckle；It is covered at a distance between side wall according to the wide adjusting of the body of fish is fixed, fixed will be covered with screw and be fixed with side wall；Experimental implementation is carried out to fish body thorax abdomen side in the fixed fish chest operation window covered；Experimental implementation is carried out to fish thorax abdomen bottom surface at fish belly portion operation window in the sidewall.The present invention can fix various sizes of fish body, facilitate operation, using simple.
Description
Technical field
The present invention relates to BIOLOGICAL CONTROL field, fixation device when especially a kind of pair of fish thorax abdomen carries out experimental implementation and
Implementation method.
Background technique
Brain is higher nervous center, and brain kinesitherapy nerve core group can control the movement of animal, so commonly used in the world
Brain control technical controlling animal robot.Brain control technology is the key technology of animal robot BIOLOGICAL CONTROL, wherein brain tissue slice
It is the important content of brain control technology, extracts the premise that brain tissue sample is then brain tissue slice preparation.
Fixation is to extract a kind of effective new method of good brain tissue sample before brain tissue sample, and effect is better than biography
Fixation after the brain tissue sample of system.Fixation is to carry out heart filling with fixer under Animal Anesthesia state before brain tissue sample
Flow complete and brain tissue sample in good condition a kind of experimental method in the form of obtaining.Carry out cardiac perfusion need to fish thorax abdomen into
Row dissection operates exposure heart, but fish body surface is smooth, is not fixed easily, and operating difficulties, spends the time long, influences experiment behaviour
Make, therefore a kind of need to meet the fish fixing device and implementation method of fixation requirement of experiment before brain tissue sample.
Adjustable fish body fixer [J] education is studied with equipment, and 2016 (9): 66-67 is by rectangle sink, one
Block L shape partition, one piece of U-shaped partition three parts composition, in addition also use a buckle, a removable bolt, for observing fish
Or probe into fish movement.
A kind of " fish fixing device of fish pretreatment machine " of 201467895 U of patent No. CN includes to fish head portion and fish
Body device for positioning and securing so that it is mechanical in the scaling of fish, cutting, remove internal organ, remove black film etc. during fish effectively position consolidate
It is fixed.
" a kind of detection fish sampling fixing device and implementation method " of 104359706 A of patent No. CN include substrate, solid
Determine card slot and fixed steel bar, the fish of sampling be fixed in the sampling of food safety detection fish, convenient for smoothly complete scale,
Peeling and sampling operation, increase convenience and the safety of sampling operation.
" the Domestic simple and easy fish auxiliary fixture of patent No. CN 205143373U
That kills " disclose a kind of fish auxiliary clamp easy to use, the device using three connecting rods, spring, arc clamping plate come
Fixed fish, conveniently butchers.
Although being directed to fish chest and abdomen in conclusion having fixation and processing that the fixed device of some fish is used for fish now
Portion carries out dissection operation for fixation device and implementation method fixed before brain tissue sample there is not yet document report, so research
It is a kind of it is easy to operate, using simple, and the fixed device of fish and implementation method that help to improve experiment accuracy are necessary.
Summary of the invention
It is an object of that present invention to provide one kind various sizes of fish body can be fixed, convenient for the thorax abdomen to fish into
Dissection of going operates, implements the fish fixing device and implementation method for fixing before brain tissue sample of cardiac perfusion.
To achieve the above object, following technical scheme is used: of the present invention a kind of for fixed before brain tissue sample
Fish fixing device, mainly include the rectangle shell structure being made of bottom plate, side wall and fixed lid, in four Bian Shangan of bottom plate
Side wall is filled, the fixed lid of installation at the top of bottom plate is equipped with the U-shaped partition of slidably adjusting position in the inside of shell structure；?
Fish belly portion operation window is opened up on one of side wall；The fish chest operation window for opening up gap shape or window-like is covered fixing.
Further, several thread grooves are opened up along the top margin of side wall, opens up spiral shell at the corresponding each screw thread groove location of fixed lid
Line mounting hole, screw, which will be connected across mounting hole with thread groove, fixed to be covered, side wall links together.
Further, the both ends raised section of the U-shaped partition fits with adjacent side wall, and in the protrusion of U-shaped partition
Buckle is installed, buckle is connected with side wall to be fixed in section.
Further, the surface of the bottom plate is coarse non slip surface.
The fixed implementation method of fixed fish before a kind of sample for brain tissue of the present invention, comprising the following steps:
Step 1, fish is subjected to dipping anesthesia；
Step 2, postanesthetic fish is lain against on the bottom plate in fixed device；
Step 3, according to the U-shaped partition of body top adjustment of fish on side wall at a distance from fish belly portion operation window, position is used after determining
Buckle fixes U-shaped partition with side wall；
Step 4, at a distance from according to the fixed lid of the wide adjusting of the body of fish between side wall, position determine after with screw will fixed lid with
Side wall is fixed, and pushes down fish body using fixed lid to complete the fixation to fish；
Step 5, experimental implementation is carried out to fish body thorax abdomen side in the fixed fish chest operation window covered；
Step 6, experimental implementation is carried out to fish thorax abdomen bottom surface at fish belly portion operation window in the sidewall.
Compared with prior art, the present invention has the advantage that
1, experiment behaviour is conveniently carried out respectively to the side of fish thorax abdomen and bottom surface positioned at 2 operation windows of side wall and fixed lid
Make；
2, the distance between lid and fish body can be fixed according to the wide adjusting of body of fish and be screwed, make fixed device height
It is adjustable；It can be fixed according to the distance between the U-shaped partition of body top adjustment of fish and side wall and with buckle, make fixed device width can
It adjusts, can arbitrarily be adjusted according to fish body size in practical application.
Detailed description of the invention
Fig. 1 is the diagrammatic perspective view of apparatus of the present invention.
Fig. 2 is the schematic diagram of internal structure of apparatus of the present invention.
Fig. 3 is the fixation lid figure of apparatus of the present invention.
Fig. 4 is the U-shaped partition figure of apparatus of the present invention.
Drawing reference numeral: 1- side wall, 2- bottom plate, 3- fish belly portion operation window, 4-U type partition, 5- buckle, the fixed lid of 6-, 7- spiral shell
Silk, 8- fish chest operation window.
Specific embodiment
The present invention will be further described with reference to the accompanying drawing:
As shown in Figs 1-4, the invention mainly comprises the rectangle shell structure being made of bottom plate 2, side wall 1 and fixed lid 6,
Side wall is installed on four sides of bottom plate, the fixed lid of installation, is equipped in the inside of shell structure and slidably adjusts at the top of bottom plate
The U-shaped partition 4 of position；Fish belly portion operation window 3 is opened up on a side wall wherein；Gap shape or window-like are opened up in fixed cover
Fish chest operation window 8.Several thread grooves are opened up along the top margin of side wall, open up screw thread at the corresponding each screw thread groove location of fixed lid
Mounting hole, screw 7, which will be connected across mounting hole with thread groove, fixed to be covered, side wall links together.The both ends of the U-shaped partition are convex
It plays section to fit with adjacent side wall, and the installation buckle 5 on the raised section of U-shaped partition, buckle is connected with side wall to be fixed.
The surface of the bottom plate is coarse non slip surface, and coarse non slip surface can increase the frictional force between fish body and bottom plate, improves stability.
Implementation method of the present invention, comprising the following steps:
1, select a tail health carp, the high 11cm of body, the wide 6cm of body, the long 34.7cm of body, the body it is a length of from carp rhynchodaenm to
Fish scale is placed in progress dipping anesthesia in cloves phenol solution at a distance from tail fin intersection, by carp.
2, laying flat the carp of anesthesia into volume is 50 × 20 × 5cm3Fixation device in bottom plate on.
3, a length of 50cm of U-shaped partition, shorter two side length is 2cm, according between the U-shaped partition of body top adjustment and side wall of fish
Distance, and fixed with buckle.
4, fixed capping product is 50 × 20cm2, fixed lid is covered, according between the fixed lid of the wide adjusting of the body of fish and side wall
Distance is simultaneously screwed, to complete the fixation to experiment carp.
5, fish chest operation window ara is 8 × 6cm2, it is 6cm away from fixed caping angular distance, passes through fish chest operation window pair
The side of fish thorax abdomen carries out experimental implementation.
6, operation window ara in fish belly portion is 8 × 1cm2, it is corresponding with fish chest operation window position, pass through fish belly portion operation window
Experimental implementation is carried out to the bottom surface of fish thorax abdomen.
Embodiment described above only describe the preferred embodiments of the invention, not to model of the invention
It encloses and is defined, without departing from the spirit of the design of the present invention, those of ordinary skill in the art are to technical side of the invention
The various changes and improvements that case is made should all be fallen into the protection scope that claims of the present invention determines.
Claims (2)
1. fixed fish fixing device before a kind of sample for brain tissue mainly includes being made of bottom plate, side wall and fixed lid
Rectangle shell structure, it is characterised in that: install side wall on four sides of bottom plate, the fixed lid of installation at the top of bottom plate, in shell
The inside of body structure is equipped with the U-shaped partition of slidably adjusting position；Fish belly portion operation window is opened up on a side wall wherein；Solid
Surely the fish chest operation window for opening up gap shape or window-like is covered, opens up several thread grooves along the top margin of side wall, in fixed lid pair
It answers and opens up threaded mounting hole at each screw thread groove location, screw, which will be connected across mounting hole with thread groove, fixed to be covered, side wall is connected to
Together, the both ends raised section of the U-shaped partition fits with adjacent side wall, and buckle is installed on the raised section of U-shaped partition,
Buckle is connected with side wall to be fixed.
2. fixed fish fixing device before a kind of sample for brain tissue according to claim 1, it is characterised in that: described
The surface of bottom plate is coarse non slip surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711094762.2A CN107647931B (en) | 2017-11-09 | 2017-11-09 | Fixed fish fixing device and implementation method before a kind of sample for brain tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711094762.2A CN107647931B (en) | 2017-11-09 | 2017-11-09 | Fixed fish fixing device and implementation method before a kind of sample for brain tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107647931A CN107647931A (en) | 2018-02-02 |
| CN107647931B true CN107647931B (en) | 2019-07-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711094762.2A Active CN107647931B (en) | 2017-11-09 | 2017-11-09 | Fixed fish fixing device and implementation method before a kind of sample for brain tissue |
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| CN (1) | CN107647931B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87210906U (en) * | 1987-07-23 | 1988-04-27 | 廖信汤 | Combined fish sieve |
| DE102010032673A1 (en) * | 2010-03-13 | 2011-09-15 | Siegfried Beck | Axial, cylindrical pipe construction for use in fish cage, has ballast chambers that are stable and flexible in axial, cylindrical sectional spaces, where ballast chambers control complete cage only with corresponding air volume |
| CN203748465U (en) * | 2014-04-09 | 2014-08-06 | 中国水产科学研究院黑龙江水产研究所 | Anti-jumping fixing device for aquarium fish culture |
| CN104257439A (en) * | 2014-10-28 | 2015-01-07 | 沈阳医学院 | Enclosed animal experiment operation box |
| CN104568542A (en) * | 2014-12-30 | 2015-04-29 | 燕山大学 | Method for fixing fish brain tissue specimen through systemic heart perfusion |
| CN105309380A (en) * | 2014-08-04 | 2016-02-10 | 四川省鸿祥科技有限公司 | Conveniently-assembled stereoscopic turtle box |
| CN106473828A (en) * | 2015-09-02 | 2017-03-08 | 中国长江三峡集团公司中华鲟研究所 | A kind of mandarin sturgeon brain tissue sample sampling bench |
| CN206102774U (en) * | 2016-07-27 | 2017-04-19 | 贵州大学 | Fish is with muscle blood collection system |
| CN206151626U (en) * | 2016-08-18 | 2017-05-10 | 中国水产科学研究院黑龙江水产研究所 | Live fish inspection underwater operation platform |
-
2017
- 2017-11-09 CN CN201711094762.2A patent/CN107647931B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87210906U (en) * | 1987-07-23 | 1988-04-27 | 廖信汤 | Combined fish sieve |
| DE102010032673A1 (en) * | 2010-03-13 | 2011-09-15 | Siegfried Beck | Axial, cylindrical pipe construction for use in fish cage, has ballast chambers that are stable and flexible in axial, cylindrical sectional spaces, where ballast chambers control complete cage only with corresponding air volume |
| CN203748465U (en) * | 2014-04-09 | 2014-08-06 | 中国水产科学研究院黑龙江水产研究所 | Anti-jumping fixing device for aquarium fish culture |
| CN105309380A (en) * | 2014-08-04 | 2016-02-10 | 四川省鸿祥科技有限公司 | Conveniently-assembled stereoscopic turtle box |
| CN104257439A (en) * | 2014-10-28 | 2015-01-07 | 沈阳医学院 | Enclosed animal experiment operation box |
| CN104568542A (en) * | 2014-12-30 | 2015-04-29 | 燕山大学 | Method for fixing fish brain tissue specimen through systemic heart perfusion |
| CN106473828A (en) * | 2015-09-02 | 2017-03-08 | 中国长江三峡集团公司中华鲟研究所 | A kind of mandarin sturgeon brain tissue sample sampling bench |
| CN206102774U (en) * | 2016-07-27 | 2017-04-19 | 贵州大学 | Fish is with muscle blood collection system |
| CN206151626U (en) * | 2016-08-18 | 2017-05-10 | 中国水产科学研究院黑龙江水产研究所 | Live fish inspection underwater operation platform |
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| CN107647931A (en) | 2018-02-02 |
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