CN107647153B

CN107647153B - Parasite expelling biscuit for shepherd dogs in Tibetan region and preparation method thereof

Info

Publication number: CN107647153B
Application number: CN201710971151.5A
Authority: CN
Inventors: 李家奎; 王蒙; 张丽鸿; 童小乐; 张辉
Original assignee: Xizang Agriculture and Animal Husbandry College
Current assignee: Tibet Liangan Biotechnology Co ltd
Priority date: 2017-10-18
Filing date: 2017-10-18
Publication date: 2021-07-02
Anticipated expiration: 2037-10-18
Also published as: CN107647153A

Abstract

The invention provides an insect-expelling biscuit for shepherd dogs in a Tibetan region and a preparation method thereof, belonging to the technical field of pet foods. The parasite expelling biscuit for shepherd dogs in the Tibetan region comprises the following components in parts by weight: 30-50 parts of dry yak meat powder, 30-50 parts of meat bone meal, 20-40 parts of high gluten flour, 20-40 parts of Zanba, 14-18 parts of white granulated sugar, 14-18 parts of water, 8-12 parts of beef tallow, 6-10 parts of praziquantel, 2-4 parts of butter, 1-3 parts of soybean oil, 0.8-1 part of fish meal and 0.1-0.3 part of acetamido abamectin. The praziquantel and the acetamido abamectin are matched to have synergistic insecticidal action, and the components such as tsamba, high gluten flour, yak meat dry powder and the like are matched to effectively improve the palatability of insect-repelling active ingredients, facilitate the ingestion of shepherd dogs, improve the nutritional value of insect-repelling biscuits, provide the immunity of the shepherd dogs and further improve the resistance of diseases.

Description

Parasite expelling biscuit for shepherd dogs in Tibetan region and preparation method thereof

Technical Field

The invention belongs to the technical field of pet foods, and particularly relates to an insect-expelling biscuit for shepherd dogs in a Tibetan region and a preparation method thereof.

Background

Parasitic diseases are diseases that seriously harm human health and livestock developing in animal husbandry, and the high incidence of diseases caused by parasites in the Tibet area has seriously affected the life and property safety of local herdsmen and the development of the breeding industry thereof. In a pasturing area (such as a Tibet glossy ganoderma pasturing area), most of shepherd dogs are raised in a scattered mode, nearly 64% of shepherd dogs do not expel insects, most of shepherd dogs have untreated excrement, and the shepherd dogs infected with echinococcus granulosus scatter eggs to the surrounding environment through the excrement, so that the shepherd dogs become a main infection source. On the other hand, extensive livestock breeding and management means also provide conditions for the spread of parasitic diseases.

The treatment of the parasitic diseases is mainly surgical operation and assisted by drug treatment, but because of the complexity of the parasitic diseases, the surgical radical treatment is difficult, or secondary or even multiple operations are needed for the after-operation repeated hair, and the treatment effect is not satisfactory. Therefore, repelling insects and controlling dogs are the main means for effective control of parasites.

At present, the drugs for expelling parasites in animals are mainly traditional Chinese medicine compositions, for example, a patent with application publication number CN102846893 discloses that the traditional Chinese medicine composition formed by combining Chinese pulsatilla root, areca seed, dark plum leaf, folium artemisiae argyi, red halloysite, golden cypress, purslane and common cnidium fruit according to certain parts by weight can treat animal parasitic diseases, and the traditional Chinese medicine composition does not generate drug resistance and drug residue and toxic and side effects. However, the traditional Chinese medicine composition has large medicinal taste and poor animal palatability, so that animals are difficult to take actively, and the later period needs to be filled with medicines, so that the operation is complex and the workload is large.

Disclosure of Invention

In view of the above, the invention aims to provide an insect-expelling biscuit for shepherd dogs in a Tibet region, and a preparation method and application thereof.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides an insect-repellent biscuit for shepherd dogs in a Tibetan region, which comprises the following components in parts by weight:

30-50 parts of dry yak meat powder, 30-50 parts of meat bone meal, 20-40 parts of high gluten flour, 20-40 parts of Zanba, 14-18 parts of white granulated sugar, 14-18 parts of water, 8-12 parts of beef tallow, 6-10 parts of praziquantel, 2-4 parts of butter, 1-3 parts of soybean oil, 0.8-1 part of fish meal and 0.1-0.3 part of acetamido abamectin; the water content of the insect-repellent biscuit is 4-6.5%.

Preferably, the composition also comprises the following components in parts by weight: 1-3 parts of spice food additive, 1-3 parts of preservative and 0.05-0.15 part of gel thickener.

Preferably, the composition comprises the following components in parts by weight: 40 parts of dry yak meat powder, 40 parts of meat bone meal, 30 parts of high gluten flour, 30 parts of Zanba, 16 parts of white granulated sugar, 16 parts of water, 10 parts of beef tallow, 8 parts of praziquantel, 3 parts of ghee, 2 parts of soybean oil, 0.9 part of fish meal, 0.2 part of acetamido abamectin, 2 parts of a spice food additive, 2 parts of a preservative and 0.1 part of a gel thickener.

The invention provides a preparation method of an insect-repellent biscuit, which comprises the following steps:

1) mixing dry yak meat powder, meat bone meal, white granulated sugar and fish meal to obtain a first mixture;

2) mixing the strong flour and the Zanba to obtain a second mixture;

3) mixing beef tallow and soybean oil, and heating to obtain a third mixture;

4) mixing praziquantel and acetamido abamectin to obtain a fourth mixture;

5) mixing the first mixture in the step 1) and the third mixture in the step 3) to obtain a fifth mixture;

6) mixing the second mixture obtained in the step 2) with shortening to obtain a sixth mixture;

7) mixing the fourth mixture in the step 4), the fifth mixture in the step 5) and the sixth mixture in the step 6) to obtain a seventh mixture;

8) mixing the seventh mixture obtained in the step 6) with water at the temperature of 50-65 ℃, filling the obtained mixed wet material into a mold, and drying to obtain the anthelmintic biscuits for shepherds in the Tibetan region;

there is no time sequence restriction between the step 1), the step 2), the step 3) and the step 4).

Preferably, when the repellent biscuit further comprises a spice food additive, a preservative and a gel thickener, the preservative is mixed with praziquantel and acetamidoabamectin to obtain a fourth mixture; mixing the gel thickener with water at the temperature of 50-65 ℃ and a seventh mixture to obtain a mixed wet material; the spice food additive is mixed with dry yak meat powder, meat and bone meal, white granulated sugar and fish meal to obtain a first mixture.

Preferably, the drying temperature in the step 8) is 65-75 ℃; the drying time is preferably 7-9 h.

Preferably, demolding is carried out when the drying is carried out for 3-4 hours, the drying is continued after demolding, and the turnover is carried out once every 1-1.5 hours after demolding.

The invention provides a shepherd dog parasite expelling biscuit in a Tibetan region, which takes praziquantel and acetamido abamectin as parasite expelling active ingredients, the praziquantel and the acetamido abamectin are matched for use, so that the parasite expelling biscuit has synergistic parasite expelling effect, the parasite expelling effect on the shepherd dog in the Tibetan region is greatly improved, and meanwhile, the parasite expelling active ingredients are matched for use with tsamba, high gluten flour, yak meat dry powder, fish meal, meat and bone meal, white granulated sugar, beef tallow and soybean oil, so that the palatability of the parasite expelling active ingredients can be improved, the feed intake of the shepherd dog is improved, the nutritional value of the parasite expelling biscuit is improved, the immunity of the shepherd dog is provided, and the disease resistance is improved. The parasite expelling biscuit provided by the invention improves the feed intake of shepherd dogs by 10-15%, and meanwhile, the parasite expelling effect reaches 85-100%, and compared with biscuits prepared by individual insecticides of praziquantel and acetamido abamectin, the parasite expelling effect is improved by 15-36%.

Detailed Description

The insect-expelling biscuit for shepherd dogs in the Tibetan region comprises dry yak meat powder. The dried yak meat powder comprises, by weight, 30-50 parts of dried yak meat powder, and preferably 40 parts of dried yak meat powder. The dried yak meat powder is sweet in taste and high in nutritive value, contains rich proteins, fat, vitamin B group, nicotinic acid, calcium, phosphorus, iron, cholesterol and other components, and has the functions of strengthening tendons and bones, tonifying deficiency, nourishing blood, eliminating phlegm, calming endogenous wind, nourishing spleen and stomach, quenching thirst and stopping salivation. The dried yak meat powder is obtained by crushing dried yak meat. The particle size of the dried yak meat powder is preferably 40-80 meshes, and more preferably 60 meshes. The method of pulverization is not particularly limited in the present invention, and pulverization means known to those skilled in the art may be used. The source of the dried yak beef is not particularly limited, and the dried yak beef which is well known in the art can be used. In the embodiment of the invention, the yak beef jerky is purchased from a farm trade market in the Yiba prefecture of Tibet.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises meat and bone meal. The meat and bone meal is 30-50 parts by weight, preferably 40 parts by weight. The meat and bone meal is an animal protein product and provides energy for the body of the shepherd dog. The particle size of the meat and bone meal is preferably 20-80 meshes, and more preferably 40 meshes. The source of the meat and bone powder is not particularly limited, and meat and bone powder well known in the art can be used. In the present examples, the meat and bone meal was purchased from lambkin pet food company.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises high-gluten flour. The high gluten flour is 20-40 parts by weight, preferably 30 parts by weight. The high gluten flour is dark in color, active and smooth, high in protein content, and capable of providing nutrition and serving as a binder and a disintegrating agent. The source of the strong flour is not particularly limited, and a strong flour well known in the art may be used. In the embodiment of the invention, the high gluten flour is purchased from food city of university of agriculture in China.

The invention provides a Tibetan region shepherd dog parasite expelling biscuit which comprises components of a Zanba. The amount of the Zanba is 20 to 40 parts by weight, preferably 30 parts by weight. The Zanba contains protein as main component 13.5 wt%, fat 2 wt%, carbohydrate 75 wt% and seventeen kinds of amino acid 14 wt%; the inorganic salts are rich in various trace elements such as calcium, iron, zinc and the like; is also rich in vitamins Bl and the like, and can basically meet the nutritional requirements of shepherd dogs. The source of the tsamba is not particularly limited, and a tsamba known in the art may be used. In the embodiment of the invention, the Zanba is purchased from the farm market in Tibet Bay.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises the components of white granulated sugar. The high white granulated sugar is 20-40 parts by weight, preferably 30 parts by weight. The white granulated sugar supplements sugar of the biscuit and improves the palatability of the insect-repellent biscuit. The source of the white granulated sugar is not particularly limited, and the white granulated sugar known in the field can be adopted. In the embodiment of the invention, the white granulated sugar is purchased from China agriculture university education supermarkets.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises water. The water accounts for 14-18 parts by weight, and preferably 16 parts by weight. The source of the water is not particularly limited, and commercially available products well known in the art may be used.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises beef tallow. The beef tallow is 8-12 parts by weight, and preferably 10 parts by weight. The beef tallow is used as a flavor element, so that the beef tallow attracts shepherd dogs to eat, and the palatability of the insect-repellent biscuit is improved. The source of the beef tallow is not particularly limited, and beef tallow well known in the art may be used. In the embodiment of the invention, the beef tallow is purchased from a cold noodle wholesale shop.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises praziquantel. The weight portion of the praziquantel is 6-10, preferably 8. The praziquantel is an antiparasitic drug and can mainly influence the sugar metabolism process of an animal body after entering the animal body. The source of the praziquantel is not particularly limited, and praziquantel known in the art can be used. In the examples of the present invention, the praziquantel was purchased from Wuhan Fei you Tech Biotech.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises ghee. The weight portion of the butter is 2-4, preferably 3. The ghee is the essence of Tibetan food, can tonify the five internal organs, can replenish qi and blood, is rich in various vitamins and has high nutritional value. The source of the shortening is not particularly limited and may be one known in the art. In the examples of the present invention, the ghee was purchased from farm markets in tibetan prefecture.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises soybean oil. The soybean oil accounts for 1-3 parts by weight, and preferably 2 parts by weight. The soybean oil is used as a flavor element, attracts shepherd dogs to eat, and improves the palatability of the parasite expelling biscuit. The source of the soybean oil is not particularly limited, and soybean oil well known in the art may be used. In the embodiment of the invention, the soybean oil is purchased from China agriculture university education supermarkets.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises fish meal. The fish meal is 0.8-1 part by weight, preferably 0.9 part by weight. The fish meal is used as an animal protein product to provide energy for the body of the shepherd dog. The source of the fish meal is not particularly limited and soybean oil well known in the art may be used. In the present examples, the fish meal was purchased from lambkin pet feed company.

The insect-repellent biscuit for shepherd dogs in the Tibetan region comprises acetamido abamectin. According to the weight portion, the acetamido abamectin is 0.1-0.3 portion, and preferably 0.2 portion. The acetamido abamectin is combined by specific binding sites on target cells in animals, so that the release amount of r-aminobutyric acid is increased, and the insecticidal action is exerted. The source of the acetamido-avermectin is not particularly limited, and the acetamido-avermectin known in the art can be used. In the embodiment of the invention, the acetamido abamectin is purchased from Wuhanfeite Biotech.

The ingredients of the repellent biscuit for shepherd dogs in the Tibetan region preferably comprise a spice food additive. The spice food additive is 1-3 parts by weight, preferably 2 parts by weight. The spice food additive increases the flavor of the biscuit and achieves the effect of food calling. The flavor food additive is preferably Gastrodia elata-AAA. The source of the savory food additive is not particularly limited, and savory food additives well known in the art may be used. In the examples of the present invention, the spice food additive was purchased from Su's original flavor store.

The insect-repellent biscuit for shepherd dogs in the Tibetan region preferably further comprises a preservative. The preservative is 1-3 parts by weight, preferably 2 parts by weight. The preservative increases shelf life. The kind of the preservative is preferably potassium sorbate. The source of the preservative is not particularly limited, and preservatives well known in the art may be used. In the examples of the present invention, the preservative was purchased from Wuhan Fei you Te Biotech.

The components of the anthelmintic biscuits for shepherd dogs in the Tibetan region provided by the invention preferably comprise a gel thickener. The gel thickener is 0.05-0.15 part by weight, and preferably 0.1 part by weight. The gel thickener has excellent thickening property, suspension property, emulsifying property and water solubility, has good heat and acid-base stability, and can enhance the hardness of the insect-repellent biscuit. The type of gel thickener is preferably xanthan gum. The source of the gel thickener is not particularly limited, and a gel thickener well known in the art may be used. In the examples of the present invention, the gel thickener was purchased from Wuhan Fei you Te Biotech.

1) mixing the strong flour and the Zanba to obtain a second mixture;

2) mixing beef tallow and soybean oil, and heating to obtain a third mixture;

3) mixing praziquantel and acetamido abamectin to obtain a fourth mixture;

4) mixing the first mixture in the step 1) and the third mixture in the step 3) to obtain a fifth mixture;

5) mixing the second mixture obtained in the step 2) with shortening to obtain a sixth mixture;

6) mixing the fourth mixture in the step 4), the fifth mixture in the step 5) and the sixth mixture in the step 6) to obtain a seventh mixture;

7) mixing the seventh mixture obtained in the step 6) with water at the temperature of 50-65 ℃, filling the obtained mixture into a mold, and drying to obtain the anthelmintic biscuits for shepherds in the Tibetan region;

there is no time sequence limit between the step 1), the step 2), the step 3) and the step 4).

The preparation method comprises the steps of mixing dry yak meat powder, meat bone meal, white granulated sugar and fish meal to obtain a first mixture; mixing the strong flour and the Zanba to obtain a second mixture; mixing beef tallow and soybean oil, and heating to obtain a third mixture; mixing praziquantel and acetamido abamectin to obtain a fourth mixture. In the present invention, the mixing in the above-mentioned preparation of the first mixture, the second mixture, the third mixture and the fourth mixture may specifically and independently adopt one or more of a cone stacking quartering method, a rolling method or a grinding method. In the invention, the heating temperature during the preparation of the third mixture is preferably 28-35 ℃, more preferably 30-33 ℃, and most preferably 32 ℃. The present invention does not limit the preparation of the first mixture, the second mixture, the third mixture and the fourth mixture in any chronological order.

After the first mixture and the third mixture are obtained, the first mixture and the third mixture are mixed to obtain a fifth mixture. The mixing method is not particularly limited, and the mixing method known to those skilled in the art can be adopted.

After the second mixture is obtained, the present invention mixes the second mixture with shortening to obtain a sixth mixture. In the present invention, the mixing process for preparing the sixth mixture is preferably accompanied by stirring. The rotating speed of the stirring is 50-100 rpm, and more preferably 80 rpm. The stirring method of the present invention is not particularly limited, and a stirring scheme known to those skilled in the art can be used. The stirring is preferably terminated until the mixture is a homogeneous yellowish powder.

After obtaining a fourth mixture, a fifth mixture, and a sixth mixture, the present invention mixes the fourth mixture, the fifth mixture, and the sixth mixture to obtain a seventh mixture. In the present invention, the mixing for preparing the seventh mixture is preferably accompanied by stirring. The stirring time is preferably 12-18 min, and more preferably 15 min. The rotating speed of the stirring is 50-100 rpm, and more preferably 80 rpm. In the present invention, after completion of the stirring, it is preferable to perform the re-mixing. The remixing mode is preferably that the stirring product is divided into four parts by a cone stacking quartering method, then the four parts are respectively placed in a mortar for grinding, and finally, the rolling mixing is carried out. The tumbling time is preferably 15-25 min, and more preferably 20 min. In the present invention, the number of remixing is preferably 2 to 3.

In the present invention, when the anthelmintic biscuit further comprises a flavor food additive, a preservative and a gel thickener, the preservative is mixed with praziquantel and acetamidoavermectin to give a fourth mixture; mixing the gel thickener with water at the temperature of 60 ℃ and a seventh mixture to obtain a mixed wet material; the spice food additive is mixed with dry yak meat powder, meat and bone meal, white granulated sugar and fish meal to obtain a first mixture.

And (3) mixing the seventh mixture with water at the temperature of 50-65 ℃ after obtaining the seventh mixture, filling the obtained mixed wet material into a mold, and drying to obtain the anthelmintic biscuits for shepherd dogs in the Tibetan region.

The mixing manner of the seventh mixture and water is not particularly limited in the present invention, and the mixing manner known to those skilled in the art can be adopted. The adding mass of the water accounts for 20-30% of that of the seventh mixture. The temperature of the water is preferably 60 ℃.

In the invention, the specification of each mould is preferably 4-6 g/mould, and more preferably 4.5 g/mould. The present invention is not particularly limited to the shape, and may employ biscuit shapes well known to those skilled in the art.

In the invention, the drying temperature is preferably 65-75 ℃, and more preferably 70 ℃. The drying time is preferably 7-9 h, and more preferably 8 h. And when the drying is carried out for 3-4 hours, preferably demoulding, and turning over once every 1-1.5 hours after demoulding.

The invention provides application of the parasite expelling biscuit or the parasite expelling biscuit prepared by the method in expelling parasites of shepherd dogs.

In the present invention, the shepherd dog parasites preferably comprise cestodes and worms. The tapeworm is preferably Echinococcus granulosus (Echinococcusgranulosus).

In the invention, when the parasite of the shepherd dog is eliminated, the quantity of the parasite is preferably 3-5 g per meal, and more preferably 4.5g per meal. Preferably 2-3 meals a day. The feeding time of the insect-repellent biscuits is preferably 1-3 d, and more preferably 1 d.

The present invention provides a anthelmintic shepherd dog biscuit for Tibetan region and its preparation method and application, which are described in detail below with reference to the examples, but they should not be construed as limiting the scope of the present invention.

Example 1

The anthelmintic biscuits for shepherd dogs in the Tibetan region are prepared by respectively weighing the following components:

30g of dried yak meat powder, 30g of meat bone meal, 20g of high gluten flour, 20g of Zanba, 14g of white granulated sugar, 14g of water, 8g of beef tallow, 6g of praziquantel, 2g of ghee, 1g of soybean oil, 1g of Gaultheria Yunnanensis-AAA 1g, 1g of potassium sorbate, 0.8g of fish meal, 0.1g of acetamido abamectin and 0.05g of xanthan gum.

The preparation method of the parasite expelling biscuits for shepherd dogs in the Tibetan region comprises the following steps:

1. weighing dried yak beef, meat and bone meal, white granulated sugar, Ganguxiang-AAA and fish meal according to the proportion, placing the dried yak beef, the meat and bone meal, the white granulated sugar, the Gaoba and the Zanba in a beaker A, placing the ghee in a beaker C, placing the beef tallow and the soybean oil in a beaker D, placing the potassium sorbate, the praziquantel and the acetamido abamectin in a beaker E, placing the xanthan gum in a beaker F, and placing the 60 ℃ warm water in a beaker G;

2. the contents in the beaker A, B, E were mixed uniformly by stacking, tumbling and grinding (repeated 2 times); pouring xanthan gum into a beaker G, mixing with water and stirring with a glass rod for 30s (37 ℃ incubation);

3. heating the soybean oil and the beef tallow together until the beef tallow is completely melted, adding the mixture into a beaker A, firstly stirring for preliminary mixing for about 10min, then respectively placing the mixture into a mortar for grinding after quartering by a stacking cone quartering method, finally rolling and uniformly mixing the mixture in the beaker A for 10min, and repeating the steps for 1 time;

4. adding butter into the beaker B, and stirring by hand while adding till the mixture is uniform light yellow powder;

5. putting all the contents in the beaker A, B, E into a 2000ml beaker H, firstly carrying out primary mixing by stirring for about 15min, then respectively putting the mixture into a mortar for grinding after quartering by a stacking cone quartering method, finally rolling and uniformly mixing the mixture in the beaker H for 20min, and repeating the steps for 1 time;

6. and adding about 5ml of liquid in the beaker G into the beaker H, bonding the contents into a dough shape, placing the dough in a selected mould, drying the dough in an air-blowing drying oven at 70 ℃, demoulding after about 4.5H, turning over once every 1H, and taking out the air-blowing drying oven after about 3.5H to obtain the insect-repellent biscuit.

Example 2

40g of dried yak meat powder, 40g of meat bone meal, 30g of strong flour, 30g of Zanba, 16g of white granulated sugar, 16g of water, 10g of beef tallow, 8g of praziquantel, 3g of ghee, 2g of soybean oil, 2g of Gaultheria odorata-AAA 2g, 2g of potassium sorbate, 0.9g of fish meal, 0.2g of acetamido abamectin and 0.1g of xanthan gum which are crushed by a crusher.

3. heating the soybean oil and the beef tallow together until the beef tallow is completely melted, adding the mixture into a beaker A, primarily mixing the mixture by stirring for about 12min, then respectively placing the mixture into a mortar for grinding after quartering by a stacking cone quartering method, finally rolling and uniformly mixing the mixture in the beaker A for 8min, and repeating the steps for 1 time;

5. putting all the contents in the beaker A, B, E into a 2000ml beaker H, firstly carrying out preliminary mixing by stirring for about 20min, then respectively putting the mixture into a mortar for grinding after quartering by a stacking cone quartering method, finally rolling and uniformly mixing the mixture in the beaker H for 15min, and repeating the steps for 1 time;

6. and adding about 8ml of liquid in the beaker G into the beaker H, bonding the contents into a dough shape, placing the dough in a selected mould, placing the mould into an air-blowing drying oven for drying at 75 ℃, demoulding after about 4H, then turning over once every 1.5 intervals, and taking out the air-blowing drying oven after about 4H to obtain the insect-repellent biscuit.

Example 3

50g of yak meat dry powder crushed by a crusher, 50g of meat bone meal, 40g of high gluten flour, 40g of Zanba, 18g of white granulated sugar, 18g of water, 12g of beef tallow, 10g of praziquantel, 4g of ghee, 3g of soybean oil, 1g of fish meal and 0.3g of acetamido abamectin.

1. weighing dried yak beef, meat and bone meal, white granulated sugar and fish meal according to the proportion, placing the dried yak beef, the meat and bone meal, the white granulated sugar and the fish meal in a beaker A, placing the strong flour and the Zanba in a beaker B, placing the ghee in a beaker C, placing the beef tallow and the soybean oil in a beaker D, placing the praziquantel and the acetamido abamectin in a beaker E, and placing the 60-DEG C warm water in a beaker G;

5. putting all the contents in the beaker A, B, E into a 2000ml beaker H, firstly carrying out primary mixing by stirring for about 8min, then respectively putting the mixture into a mortar for grinding after quartering by a stacking cone quartering method, finally rolling and uniformly mixing the mixture in the beaker H for 18min, and repeating the steps for 1 time;

6. and adding about 5ml of liquid in the beaker G into the beaker H, bonding the contents into a dough shape, placing the dough in a selected mould, drying the dough in an air-blast drying oven at 70 ℃, demoulding after about 3H, turning over once every 1H, and taking out the air-blast drying oven after about 4.5H to obtain the insect-repellent biscuit.

Comparative example 1

The insect-expelling biscuits prepared by the method of the example 3 are prepared by using starch and salt as auxiliary materials to replace yak meat dry powder, meat bone meal, high gluten flour, tsamba, white granulated sugar, beef tallow, ghee, soybean oil and fish meal in the example 3, wherein the mass ratio of the starch to the salt is 20:1, and the prepared biscuits are compared with the feed intake of the biscuits prepared in the examples 1-3.

The experimental method comprises the following steps: one feeding basin was provided for each of the 5 experimental shepherd dogs at the same time, and a known amount of the biscuits prepared in example 1, example 2, example 3 and comparative example 1 to be tested was placed in the feeding basin. And weighing and recording the feed intake of the insect-repellent biscuits in one feed intake process (9:00-15: 00). The results of the feed intake are shown in Table 1.

TABLE 1 feed intake for shepherd dogs on biscuits made according to different examples

Group of	Example 1	Example 2	Example 3	Comparative example 1
					Rate of food intake	72.1％	78.9％	75.2％	13.5％

Example 4

In order to test the insect-repelling effect of the combination of praziquantel and acetamidoavermectin, the biscuits prepared in example 1 were used as a combined treatment group of praziquantel and acetamidoavermectin to perform an insect-repelling experiment on the shepherd dogs with parasites, biscuits prepared in example 1 by removing praziquantel or acetamidoavermectin were used as an acetamidoavermectin treatment group and a praziquantel treatment group respectively, biscuits prepared in example 1 by removing praziquantel and eplerentin were used as a blank control group to be fed, the number of the shepherd dogs in each group was 10 as a positive control group, the biscuits were weighed and fed at the ratio of 9:00-15:00, and the insect-reducing rate of each group of striped insects and worms was counted respectively. The statistical results are shown in Table 2.

TABLE 2 reduction of cestodes and helminths in shepherd dogs by different treatment groups

Treatment group	Cestode insect reduction rate	Worm reduction rate
			Praziquantel treatment group	96.3％	80％
Acetaminoavermectin treatment group	---	62.8％
			Praziquantel and acetamido abamectin combination treatment group	97％	98.9％
Blank control group	0	0

As can be seen from Table 2, for the anthelmintic effect of tapeworm, the tapeworm reduction rate of the combination treatment group of praziquantel and acetamido abamectin is 97%, which is slightly higher than that of the praziquantel treatment group; aiming at expelling worms from worms, the helminth reduction rate of the praziquantel and acetamido abamectin combined treatment group is 98.9 percent, which is respectively improved by 23.6 percent and 57.48 percent compared with the helminth reduction rate of the praziquantel treatment group and the acetamido abamectin treatment group. It can be seen that the combined use of the praziquantel and the acetamido abamectin can greatly improve the anthelmintic effect on the worms and has the synergistic effect.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The parasite expelling biscuit for shepherd dogs in the Tibetan region is characterized by comprising the following components in parts by weight:

30-50 parts of dry yak meat powder, 30-50 parts of meat bone meal, 20-40 parts of high gluten flour, 20-40 parts of Zanba, 14-18 parts of white granulated sugar, 8-12 parts of beef tallow, 6-10 parts of praziquantel, 2-4 parts of butter, 1-3 parts of soybean oil, 0.8-1 part of fish meal, 0.1-0.3 part of acetamido abamectin, 1-3 parts of a spice food additive, 1-3 parts of a preservative and 0.05-0.15 part of a gel thickener; the water content of the insect-repellent biscuit is 4-6.5%.

2. The insect-repellent biscuit according to claim 1, comprising the following components in parts by weight: 40 parts of dry yak meat powder, 40 parts of meat bone meal, 30 parts of high gluten flour, 30 parts of Zanba, 16 parts of white granulated sugar, 16 parts of water, 10 parts of beef tallow, 8 parts of praziquantel, 3 parts of ghee, 2 parts of soybean oil, 0.9 part of fish meal, 0.2 part of acetamido abamectin, 2 parts of a spice food additive, 2 parts of a preservative and 0.1 part of a gel thickener.

3. A method of making an insect repellent biscuit according to claim 1, comprising the steps of:

2) mixing the strong flour and the Zanba to obtain a second mixture;

3) mixing beef tallow and soybean oil, and heating to obtain a third mixture;

4) mixing praziquantel and acetamido abamectin to obtain a fourth mixture;

8) mixing the seventh mixture obtained in the step 7) with water at the temperature of 50-65 ℃, filling the obtained mixed wet material into a mold, and drying to obtain the anthelmintic biscuits for shepherds in the Tibetan region;

4. The method of preparing as claimed in claim 3, wherein when said repellent biscuit further comprises a spice food additive, a preservative and a gel thickener, said preservative is mixed with praziquantel and acetamidoavermectin to obtain a fourth mixture; mixing the gel thickener with water at the temperature of 50-65 ℃ and a seventh mixture to obtain a mixed wet material; the spice food additive is mixed with dry yak meat powder, meat and bone meal, white granulated sugar and fish meal to obtain a first mixture.

5. The method according to claim 3 or 4, wherein the temperature for drying in step 8) is 65-75 ℃.

6. The preparation method according to claim 5, wherein the drying time in the step 8) is preferably 7-9 h.

7. The preparation method according to claim 5, wherein the drying is carried out for 3-4 h, demoulding is carried out, the drying is continued after demoulding, and the surface turning is carried out once every 1-1.5 h after demoulding.