CN107641616A - A kind of method that immunocyte is extracted from peripheral blood - Google Patents
A kind of method that immunocyte is extracted from peripheral blood Download PDFInfo
- Publication number
- CN107641616A CN107641616A CN201711117635.XA CN201711117635A CN107641616A CN 107641616 A CN107641616 A CN 107641616A CN 201711117635 A CN201711117635 A CN 201711117635A CN 107641616 A CN107641616 A CN 107641616A
- Authority
- CN
- China
- Prior art keywords
- parts
- immunocyte
- peripheral blood
- cooled
- extracted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of method that immunocyte is extracted from peripheral blood, comprise the following steps：(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube and centrifuge, absorption upper plasma is standby, and lower confluent monolayer cells add physiological saline, mixing, obtain cell suspension；(2) cell suspension is placed in separating liquid, centrifuges 20 30min in 600 800g, draw tunica albuginea confluent monolayer cells adaptive immune cell；(3) immunocyte is added in frozen stock solution in equal volume, it is sub-packed in cell cryopreservation tube, then cryopreservation tube is placed in program freezing storing box, first 20~25 DEG C are cooled to 12 DEG C/min, then 40~45 DEG C are cooled to 35 DEG C/min, 78~82 DEG C are cooled to 56 DEG C again, is finally transferred in liquid nitrogen and freezes.The method of the present invention can effectively provide the survival rate after immunocyte separation rate and cryopreservation, improve the security for feeding back to the cell of patient.
Description
Technical field
The invention belongs to the extractive technique field of immunocyte, and in particular to a kind of that immunocyte is extracted from peripheral blood
Method.
Background technology
Immunocyte is extracted from the peripheral blood of people, not only there is preferable prospect in terms for the treatment of cancer, and from health
From the perspective of health, immunocyte, and patient's sheet afterwards can also be extracted from its peripheral blood in sufferers themselves' health
When immunocompromised or other symptoms occurs in people, the immunocyte of storage can be fed back to sufferers themselves, improve exempting from for patient
Epidemic disease power.At present, the individual cells of peripheral blood are mainly preserved using cryopreservation technology, simultaneously Fiber differentiation is for recovery when needed
Various immunocytes, but during separating, freezing, individual cells can be produced with damage, or even toxicity, especially freeze process
The middle thermodynamics that can significantly change cell, chemically and physically environment, while with the danger of biological injury.
In addition, separating liquid used has certain toxicity to cell in the prior art, it is unfavorable for clinical practice, and cell freezes
Deposit the survival rate after recovery be also possible to can by cell collection, separate, the process such as freeze and influenceed.
The content of the invention
For above-mentioned deficiency of the prior art, the invention provides a kind of side that immunocyte is extracted from peripheral blood
Method, can effectively solve immunocyte present in prior art divides interest rate low, and the survival rate after cryopreservation is low, feeds back
The problem of security be present in the cell to patient.
To achieve the above object, the technical solution adopted for the present invention to solve the technical problems is：
A kind of method that immunocyte is extracted from peripheral blood, comprises the following steps：
(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, under
Confluent monolayer cells add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 1-5：2-4；
(2) cell suspension is placed in separating liquid, 20-30min is centrifuged in 600-800g, drawn tunica albuginea confluent monolayer cells and exempted from
Epidemic disease cell；
Wherein, separating liquid includes the component of following parts by weight：Trehalose 3-6 parts, dextran 1-3 parts, stephanine 1-3
Part, tremella polysaccharides 1-2 parts, laminarin 0.5-1.2 parts, ultra-low viscosity sodium alginate 0.2-0.5 parts and polydiene dimethylamine
Ammonium chloride 0.1-0.2 parts；
(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, be then positioned over cryopreservation tube
In program freezing storing box, -20~-25 DEG C first are cooled to 1-2 DEG C/min, is then cooled to -40~-45 DEG C again with 3-5 DEG C/min,
- 78~-82 DEG C are cooled to 5-6 DEG C again, is finally transferred in liquid nitrogen and freezes；
Wherein, frozen stock solution includes the component of following parts by weight：Trehalose 4-7 parts, dextran 3-6 parts, dimethyl sulfoxide (DMSO)
2-5 parts, Tea Polyphenols 0.3-0.8 parts, upper plasma 13-18 parts, DMSO 10-15 parts and physiological saline 55-60 parts.
Further, the volume ratio of peripheral blood and physiological saline is 2 in step (1)：3.
Further, separating liquid includes the component of following parts by weight in step (2)：5 parts of trehalose, 2 parts of dextran, thousand
Golden 2 parts of rattan alkali, 1.5 parts of tremella polysaccharides, 0.8 part of laminarin, 0.3 part of ultra-low viscosity sodium alginate and diallyl dimethyl
0.2 part of ammonium chloride.
Further, separating liquid is prepared by the following method to obtain in step (2)：Each component is mixed by weight, so
The solution that 100 parts by weight are configured in the water for injection after sterilizing is dissolved in afterwards, then by 0.22 μm of membrane filtration, is made.
Further, the density of separating liquid is 1.050-1.070g/cm in step (2)3, osmotic pressure 280-290smol/
Kg, pH 7-8, viscosity 2.0-3.0cp.
Further, frozen stock solution includes the component of following parts by weight in step (3)：5 parts of trehalose, 4 parts of dextran, two
59.5 parts of 3 parts of methyl sulfoxide, 0.5 part of Tea Polyphenols, 16 parts of upper plasma, 12 parts of DMSO and physiological saline.
Further, program is frozen in step (3) to be first cooled to -20~-25 DEG C with 2 DEG C/min, then again with 5
DEG C/min is cooled to -40~-45 DEG C, then is cooled to -78~-82 DEG C with 6 DEG C, finally it is transferred in liquid nitrogen and freezes.
Further, dextran Dextran70.
A kind of method that immunocyte is extracted from peripheral blood provided by the invention, has the advantages that：
The present invention centrifuges peripheral blood cells, a part of the upper plasma as frozen stock solution, can effectively reduce returning step
In immunological rejection effect.
Separating liquid of the present invention is combined using specific components, and its trehalose is a kind of safe and reliable natural carbohydrate, by two
The nonreducing sugar that glucose molecule is formed with 1,1- glycosidic bonds, there is non-specific protection to make to various bioactivators
With, its tremella polysaccharides and laminarin, aim cell can also be played a protective role, while can for the purpose of cell provide battalion
Support, furthermore bacteriostasis can also be played with stephanine, the separation to aim cell together with other materials is played collaboration and made
Separated well with cell is made；Ultra-low viscosity sodium alginate has relatively low viscosity, and more hydroxyl contributes to red blood cell
Precipitation；Diallyl dimethyl ammoniumchloride is strong cationic polyelectrolytes, contributes to the precipitation of red blood cell；Using this separation
After liquid carries out centrifugal treating to blood sample, lymphocyte will form ratio with monocyte in the interface of separating medium and blood plasma
Obvious cloud cell band, and other cells such as red blood cell, granulocyte are distributed in centrifuge tube bottom under the action of the centrifugal force
With separating medium layer.
Using frozen stock solution provided by the invention, aim cell can effectively be frozen in the case where specifically freezing program, and
Its loss amount can be effectively reduced, preserves its survival rate and higher activity.
Embodiment
Embodiment 1
A kind of method that immunocyte is extracted from peripheral blood, comprises the following steps：
(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, under
Confluent monolayer cells add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 1：2；
(2) cell suspension is placed in separating liquid, centrifuges 30min in 800g, draw tunica albuginea confluent monolayer cells adaptive immune cell；
Wherein, separating liquid includes the component of following parts by weight：3 parts of trehalose, 1 part of dextran (Dextran70), a thousand pieces of gold
1 part of rattan alkali, 1 part of tremella polysaccharides, 0.5 part of laminarin, 0.2 part of ultra-low viscosity sodium alginate and diallyl dimethyl chlorination
0.1 part of ammonium；
Separating liquid is prepared by the following method to obtain：Each component is mixed by weight, the note being then dissolved in after sterilizing
Penetrate and use in water, be configured to the solution of 100 parts by weight, then by 0.22 μm of membrane filtration, be made；
The density of separating liquid is 1.050-1.070g/cm3, osmotic pressure 280-290smol/kg, pH 7-8, viscosity is
2.0-3.0cp。
(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, be then positioned over cryopreservation tube
In program freezing storing box, first with 2 DEG C/min -20~-25 DEG C are cooled to, are then cooled to -40~-45 DEG C again with 5 DEG C/min, then with
6 DEG C are cooled to -78~-82 DEG C, are finally transferred in liquid nitrogen and freeze.
Wherein, frozen stock solution includes the component of following parts by weight：4 parts of trehalose, 3 parts of dextran (Dextran70), diformazan
55 parts of 2 parts of base sulfoxide, 0.3 part of Tea Polyphenols, 13 parts of upper plasma, 10 parts of DMSO and physiological saline.
Embodiment 2
A kind of method that immunocyte is extracted from peripheral blood, comprises the following steps：
(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, under
Confluent monolayer cells add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 1：4；
(2) cell suspension is placed in separating liquid, centrifuges 30min in 800g, draw tunica albuginea confluent monolayer cells adaptive immune cell；
Wherein, separating liquid includes the component of following parts by weight：6 parts of trehalose, 3 parts of dextran (Dextran70), a thousand pieces of gold
3 parts of rattan alkali, 2 parts of tremella polysaccharides, 1.2 parts of laminarin, 0.5 part of ultra-low viscosity sodium alginate and diallyl dimethyl chlorination
0.2 part of ammonium；
Separating liquid is prepared by the following method to obtain：Each component is mixed by weight, the note being then dissolved in after sterilizing
Penetrate and use in water, be configured to the solution of 100 parts by weight, then by 0.22 μm of membrane filtration, be made；
The density of separating liquid is 1.050-1.070g/cm3, osmotic pressure 280-290smol/kg, pH 7-8, viscosity is
2.0-3.0cp。
(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, be then positioned over cryopreservation tube
In program freezing storing box, first with 2 DEG C/min -20~-25 DEG C are cooled to, are then cooled to -40~-45 DEG C again with 5 DEG C/min, then with
6 DEG C are cooled to -78~-82 DEG C, are finally transferred in liquid nitrogen and freeze.
Wherein, frozen stock solution includes the component of following parts by weight：7 parts of trehalose, 6 parts of dextran (Dextran70), diformazan
60 parts of 5 parts of base sulfoxide, 0.8 part of Tea Polyphenols, 18 parts of upper plasma, 15 parts of DMSO and physiological saline.
Embodiment 3
A kind of method that immunocyte is extracted from peripheral blood, comprises the following steps：
(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, under
Confluent monolayer cells add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 1：3；
(2) cell suspension is placed in separating liquid, centrifuges 30min in 800g, draw tunica albuginea confluent monolayer cells adaptive immune cell；
Wherein, separating liquid includes the component of following parts by weight：4 parts of trehalose, 1.5 parts of dextran (Dextran70), thousand
Golden 1.5 parts of rattan alkali, 1.5 parts of tremella polysaccharides, 0.7 part of laminarin, 0.3 part of ultra-low viscosity sodium alginate and polydiene dimethylamine
0.13 part of ammonium chloride；
Separating liquid is prepared by the following method to obtain：Each component is mixed by weight, the note being then dissolved in after sterilizing
Penetrate and use in water, be configured to the solution of 100 parts by weight, then by 0.22 μm of membrane filtration, be made；
The density of separating liquid is 1.050-1.070g/cm3, osmotic pressure 280-290smol/kg, pH 7-8, viscosity is
2.0-3.0cp。
(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, be then positioned over cryopreservation tube
In program freezing storing box, first with 2 DEG C/min -20~-25 DEG C are cooled to, are then cooled to -40~-45 DEG C again with 5 DEG C/min, then with
6 DEG C are cooled to -78~-82 DEG C, are finally transferred in liquid nitrogen and freeze.
Wherein, frozen stock solution includes the component of following parts by weight：5 parts of trehalose, 4 parts of dextran (Dextran70), diformazan
57 parts of 3 parts of base sulfoxide, 0.5 part of Tea Polyphenols, 15 parts of upper plasma, 12 parts of DMSO and physiological saline.
Embodiment 4
A kind of method that immunocyte is extracted from peripheral blood, comprises the following steps：
(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, under
Confluent monolayer cells add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 2：3；
(2) cell suspension is placed in separating liquid, centrifuges 30min in 800g, draw tunica albuginea confluent monolayer cells adaptive immune cell；
Wherein, separating liquid includes the component of following parts by weight：5 parts of trehalose, 2.5 parts of dextran (Dextran70), thousand
Golden 2.5 parts of rattan alkali, 1.8 parts of tremella polysaccharides, 1.0 parts of laminarin, 0.4 part of ultra-low viscosity sodium alginate and polydiene dimethylamine
0.2 part of ammonium chloride；
Separating liquid is prepared by the following method to obtain：Each component is mixed by weight, the note being then dissolved in after sterilizing
Penetrate and use in water, be configured to the solution of 100 parts by weight, then by 0.22 μm of membrane filtration, be made；
The density of separating liquid is 1.050-1.070g/cm3, osmotic pressure 280-290smol/kg, pH 7-8, viscosity is
2.0-3.0cp。
(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, be then positioned over cryopreservation tube
In program freezing storing box, first with 2 DEG C/min -20~-25 DEG C are cooled to, are then cooled to -40~-45 DEG C again with 5 DEG C/min, then with
6 DEG C are cooled to -78~-82 DEG C, are finally transferred in liquid nitrogen and freeze.
Wherein, frozen stock solution includes the component of following parts by weight：6 parts of trehalose, 5 parts of dextran (Dextran70), diformazan
59 parts of 4 parts of base sulfoxide, 0.6 part of Tea Polyphenols, 16 parts of upper plasma, 14 parts of DMSO and physiological saline.
Embodiment 5
A kind of method that immunocyte is extracted from peripheral blood, comprises the following steps：
(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, under
Confluent monolayer cells add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 2：3；
(2) cell suspension is placed in separating liquid, centrifuges 30min in 800g, draw tunica albuginea confluent monolayer cells adaptive immune cell；
Wherein, separating liquid includes the component of following parts by weight：5 parts of trehalose, 2 parts of dextran (Dextran70), a thousand pieces of gold
2 parts of rattan alkali, 1.5 parts of tremella polysaccharides, 0.8 part of laminarin, 0.3 part of ultra-low viscosity sodium alginate and diallyl dimethyl chlorine
Change 0.2 part of ammonium；
Separating liquid is prepared by the following method to obtain：Each component is mixed by weight, the note being then dissolved in after sterilizing
Penetrate and use in water, be configured to the solution of 100 parts by weight, then by 0.22 μm of membrane filtration, be made；
The density of separating liquid is 1.050-1.070g/cm3, osmotic pressure 280-290smol/kg, pH 7-8, viscosity is
2.0-3.0cp。
(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, be then positioned over cryopreservation tube
In program freezing storing box, first with 2 DEG C/min -20~-25 DEG C are cooled to, are then cooled to -40~-45 DEG C again with 5 DEG C/min, then with
6 DEG C are cooled to -78~-82 DEG C, are finally transferred in liquid nitrogen and freeze.
Wherein, frozen stock solution includes the component of following parts by weight：5 parts of trehalose, 4 parts of dextran (Dextran70), diformazan
59.5 parts of 3 parts of base sulfoxide, 0.5 part of Tea Polyphenols, 16 parts of upper plasma, 12 parts of DMSO and physiological saline.
Comparative example 1
The difference from Example 5 of comparative example 1 is do not have stephanine, tremella polysaccharides and laminarin in separating liquid,
Remaining is same as Example 5.
Comparative example 2
The difference from Example 5 of comparative example 2 is do not have Tea Polyphenols in frozen stock solution, and remaining is same as Example 5.
Comparative example 3
The difference from Example 5 of comparative example 3 is do not have stephanine, tremella polysaccharides and laminarin in separating liquid,
There is no Tea Polyphenols in frozen stock solution, remaining is same as Example 5.
Comparative example 4
The difference from Example 5 of comparative example 4 is that it is prior to -80 DEG C freezings to freeze program, is then transferred in liquid nitrogen and freezes
Deposit, remaining is same as Example 5.
The immunocyte that embodiment 1-5 and comparative example 1-4 is extracted, froze through 1 month, 3 months, 6 months, 12 months
After recover, detect the survival rate of immunocyte, it is as a result as follows：
As seen from the above table, method of the invention can effectively provide depositing after immunocyte separation rate and cryopreservation
Motility rate, improve the security for feeding back to the cell of patient.
Claims (8)
- A kind of 1. method that immunocyte is extracted from peripheral blood, it is characterised in that comprise the following steps：(1) human peripheral is gathered, anti-coagulants is added, then moves into centrifuge tube, is centrifuged, absorption upper plasma is standby, and lower floor is thin Born of the same parents add physiological saline, mixing, obtain cell suspension；Wherein, the volume ratio of peripheral blood and physiological saline is 1-5：2-4；(2) cell suspension is placed in separating liquid, centrifuges 20-30min in 600-800g, it is thin to draw tunica albuginea confluent monolayer cells adaptive immune Born of the same parents；Wherein, separating liquid includes the component of following parts by weight：Trehalose 3-6 parts, dextran 1-3 parts, stephanine 1-3 parts, Tremella polysaccharides 1-2 parts, laminarin 0.5-1.2 parts, ultra-low viscosity sodium alginate 0.2-0.5 parts and diallyl dimethyl chlorine Change ammonium 0.1-0.2 parts；(3) immunocyte is added in frozen stock solution in equal volume, be sub-packed in cell cryopreservation tube, cryopreservation tube is then positioned over program In freezing storing box, -20~-25 DEG C first are cooled to 1-2 DEG C/min, is then cooled to -40~-45 DEG C with 3-5 DEG C/min, then with 5- 6 DEG C are cooled to -78~-82 DEG C, are finally transferred in liquid nitrogen and freeze；Wherein, frozen stock solution includes the component of following parts by weight：Trehalose 4-7 parts, dextran 3-6 parts, dimethyl sulfoxide (DMSO) 2-5 Part, Tea Polyphenols 0.3-0.8 parts, upper plasma 13-18 parts, DMSO 10-15 parts and physiological saline 55-60 parts.
- 2. the method according to claim 1 that immunocyte is extracted from peripheral blood, it is characterised in that step (1) China and foreign countries The volume ratio of all blood and physiological saline is 2：3.
- 3. the method according to claim 1 that immunocyte is extracted from peripheral blood, it is characterised in that divide in step (2) Chaotropic includes the component of following parts by weight：5 parts of trehalose, 2 parts of dextran, 2 parts of stephanine, 1.5 parts of tremella polysaccharides, sea-tangle 0.2 part of 0.8 part of polysaccharide, 0.3 part of ultra-low viscosity sodium alginate and diallyl dimethyl ammoniumchloride.
- 4. the method that immunocyte is extracted from peripheral blood according to claim 1 or 3, it is characterised in that in step (2) Separating liquid is prepared by the following method to obtain：Each component is mixed by weight, is then dissolved in the water for injection after sterilizing, The solution of 100 parts by weight is configured to, then by 0.22 μm of membrane filtration, is made.
- 5. the method according to claim 4 that immunocyte is extracted from peripheral blood, it is characterised in that divide in step (2) The density of chaotropic is 1.050-1.070g/cm3, osmotic pressure 280-290smol/kg, pH 7-8, viscosity 2.0-3.0cp.
- 6. the method according to claim 1 that immunocyte is extracted from peripheral blood, it is characterised in that freeze in step (3) Liquid storage includes the component of following parts by weight：5 parts of trehalose, 4 parts of dextran, 3 parts of dimethyl sulfoxide (DMSO), 0.5 part of Tea Polyphenols, upper strata 59.5 parts of 16 parts of blood plasma, 12 parts of DMSO and physiological saline.
- 7. the method according to claim 1 that immunocyte is extracted from peripheral blood, it is characterised in that freeze in step (3) Deposit and freeze program to be first cooled to -20~-25 DEG C with 2 DEG C/min, be then cooled to -40~-45 DEG C with 5 DEG C/min, then with 6 DEG C - 78~-82 DEG C are cooled to, is finally transferred in liquid nitrogen and freezes.
- 8. the method according to claim 1 that immunocyte is extracted from peripheral blood, it is characterised in that the dextran For Dextran70.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711117635.XA CN107641616A (en) | 2017-11-13 | 2017-11-13 | A kind of method that immunocyte is extracted from peripheral blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711117635.XA CN107641616A (en) | 2017-11-13 | 2017-11-13 | A kind of method that immunocyte is extracted from peripheral blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107641616A true CN107641616A (en) | 2018-01-30 |
Family
ID=61125837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711117635.XA Pending CN107641616A (en) | 2017-11-13 | 2017-11-13 | A kind of method that immunocyte is extracted from peripheral blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107641616A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111358808A (en) * | 2020-04-17 | 2020-07-03 | 上海健珮生物科技有限公司 | Preparation method of immune cell and plasma nano-extract liposome immunoregulation preparation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462922A (en) * | 2015-12-31 | 2016-04-06 | 广州赛莱拉干细胞科技股份有限公司 | Method for increasing yield of immune cells |
| CN105462920A (en) * | 2015-12-31 | 2016-04-06 | 北京弘润天源生物技术股份有限公司 | Method for extracting immune cells from peripheral blood |
| CN105532644A (en) * | 2015-12-31 | 2016-05-04 | 北京弘润天源生物技术股份有限公司 | Cryopreservation method for lymphocyte |
-
2017
- 2017-11-13 CN CN201711117635.XA patent/CN107641616A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462922A (en) * | 2015-12-31 | 2016-04-06 | 广州赛莱拉干细胞科技股份有限公司 | Method for increasing yield of immune cells |
| CN105462920A (en) * | 2015-12-31 | 2016-04-06 | 北京弘润天源生物技术股份有限公司 | Method for extracting immune cells from peripheral blood |
| CN105532644A (en) * | 2015-12-31 | 2016-05-04 | 北京弘润天源生物技术股份有限公司 | Cryopreservation method for lymphocyte |
Non-Patent Citations (2)
| Title |
|---|
| 梁剑平: "《中兽药学》", 31 January 2014, 军事医学科学出版社 * |
| 王建林: "《当代食品科学与技术概论》", 30 September 2005, 兰州大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111358808A (en) * | 2020-04-17 | 2020-07-03 | 上海健珮生物科技有限公司 | Preparation method of immune cell and plasma nano-extract liposome immunoregulation preparation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105543168B (en) | The storage and transportation resources of immunocyte | |
| KR920005684B1 (en) | Synthetic plasma-free transfusible platelet storage medium | |
| CN106591233B (en) | A kind of external evoked amplification of immunocyte and the method frozen | |
| CN106818710A (en) | A kind of cells frozen storing liquid and its preparation method and application | |
| CN105532644B (en) | The cryopreservation methods of lymphocyte | |
| CN101828744A (en) | Anti-fatigue total nutrition high activity seacucumber drinking liquid capable of improving immunity and preparation method thereof | |
| Gašová et al. | PBPC collection techniques: standard versus large volume leukapheresis (LVL) in donors and in patients | |
| CN105462920B (en) | The method for extracting immunocyte from peripheral blood | |
| CN110038165B (en) | Fat graft for enriching high-activity fat granular cells and fat stem cells and preparation method and application thereof | |
| CN105087483A (en) | Immune cell culture medium and culture method, as well as application of ginsenoside | |
| CN106967675A (en) | A kind of method of the separation and Extraction candidate stem cell from placenta | |
| CN107641616A (en) | A kind of method that immunocyte is extracted from peripheral blood | |
| CN104857018B (en) | Raspberry polysaccharide prepares the purposes of antineoplastic combination medication with chemotherapeutics | |
| CN105796587B (en) | Caulis bambusae in taenian polysaccharide immunological regulation, it is antitumor in application | |
| CN102432669A (en) | Purification method for extracting glycoprotein from rhopilema esculentum | |
| CN107495020A (en) | A kind of suitable polypeptide beverage water of diabetic population and its preparation technology | |
| CN108013025A (en) | A kind of frozen stock solution and its application | |
| CN105543167B (en) | The preservation and transportation resources of stem cell | |
| CN102198263B (en) | Rhizoma Dioscoreae esculentae Sporamin albumen is preparing the application in prevention and therapy tumour medicine and health product | |
| CN104115995B (en) | A kind of glycoconjugates preparation improving animal semen vigor and application thereof | |
| CN105315383A (en) | Purified active polysaccharides extracted from agaricus bisporus can processing waste water and extracting and purifying technology and application thereof | |
| CN114009425B (en) | Immune cell vitrification cryopreservation protective solution and cryopreservation method thereof | |
| CN105315382A (en) | Purified active polysaccharides extracted from agaricus bisporus can processing waste water and extracting and purifying technology and application thereof | |
| Klimberg | Autotransfusion and blood conservation in urologic oncology | |
| CN114026129B (en) | Application of glucan in preparation of medicines |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180130 |
|
| RJ01 | Rejection of invention patent application after publication |