CN107628833A - A kind of method that liquid biological organic fertilizer is produced using squid viscera - Google Patents

A kind of method that liquid biological organic fertilizer is produced using squid viscera Download PDF

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Publication number
CN107628833A
CN107628833A CN201710866859.4A CN201710866859A CN107628833A CN 107628833 A CN107628833 A CN 107628833A CN 201710866859 A CN201710866859 A CN 201710866859A CN 107628833 A CN107628833 A CN 107628833A
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squid viscera
fermentation vat
relative
fermentation
addition
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姜维
刘宇
胡世伟
李世杰
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

A kind of method that liquid biological organic fertilizer is produced using squid viscera, this method are comprised the following steps：a）Squid viscera is beaten homogeneous, pours into fermentation vat；b）Add water into fermentation vat, stir；c）It is 25 30 DEG C first to control fermentation vat temperature, adds neutral proteinase, stirs, and is digested 23 hours, then controls 40 45 DEG C of the temperature of fermentation vat, adds neutral lipase, stirs, and continues enzymolysis 35 hours；d）Added into fermentation vat relative to step（1）The sugar of middle squid viscera weight 0.5 1%, stirs；e）S. cervisiae, bacillus subtilis, Li's Trichoderma and azotobacter chroococcum are added into fermentation vat, is stirred；f）Fermentation：Heat-preservation fermentation；g）Stop stirring, stand 36 48 hours；h）Filtering：Floaters on surface is removed, takes supernatant, double gauze filtering；i）Filtrate is left and taken, is packed.

Description

A kind of method that liquid biological organic fertilizer is produced using squid viscera
Technical field
The present invention relates to a kind of method that liquid biological organic fertilizer is produced using squid viscera, belong to food processing field.
Background technology
In recent years, squid demand is increasingly increased both at home and abroad, amount of fishing stablizes increase year by year.Squid non-edible part accounts for 20% or so of weight, wherein internal organ account for 15% or so, and this part discarded object is generally buried processing, however, such place Reason mode, the waste of resource is on the one hand caused, is on the other hand also polluted the environment.According to analysis, squid viscera contains 70% or so Moisture, 13% or so protein and other compositions, how to make full use of this portion of material, for lifted squid industrial upgrading Significance is respectively provided with environmental protection.
Research was done in utilization of the someone to squid viscera.Chinese patent CN102180945A is disclosed in a kind of squid Dirty extraction small peptide and preparation method thereof, the small peptide and bean cake powder, fish meal, corn flour and starch composition, can improve taking the photograph for fish Appetite.Chinese patent CN102787110A discloses a kind of extraction process of squid viscera enzyme, and the technique is by being dehydrated, at acetone The steps such as reason, homogenate, filtering, air-dried, dissolving, refrigerated centrifuge and filtering, obtain squid viscera alkalescence enzyme liquid.Chinese patent CN103601655A discloses a kind of method that natural taurine is extracted from squid viscera, and this method is through over cleaning, homogenate, enzyme The steps such as solution, filtering, precipitation, ion exchange and crystallization, obtain taurine crystalline solid.Chinese patent CN102392010A is disclosed It is a kind of to extract obtained protease and its extracting method and application using squid viscera as raw material, this method by broken, water extraction or The steps such as salt carries, goes to precipitate, flocculate, concentrate, dry and crush, are made squid viscera protease preparation.Above-mentioned various methods can To utilize some components of squid viscera, but utilization rate is relatively low, and substantial amounts of remaining squid viscera component still needs to be handled.China Patent CN1923052A discloses a kind of comprehensive utilization technique of Peru squid internal organ, and this method is by boiling, enzyme liquid, heating And centrifugal treating, supernatant obtain refining Squid Oil by the step such as saponification, decolouring and deodorant, lower floor be concentrated in vacuo into rich in Protein component, refine Squid Oil and both function as animal feed additive rich in protein component, this method can be more fully Using squid viscera, but process is more complicated, and cost is higher.
Numerous studies show that amino acid, which has, to be promoted vine growth and development, enhancing resistance, improves soil regime and raising Crop yield effect (Wang Ying, Shi Zhensheng, Wang Zhibin, Li Feng sea plants to amino acid absorb and amino acid is in agricultural In application [J] Chinese soils and fertilizer, 2008 (1):6-11.).Liu Chune etc. is using different protease in squid viscera Protein digested, the protein in squid viscera can be changed into amino acid to a certain extent, however, being used only In the case of protease, conversion ratio is than relatively low (Liu Chune, Lin Hong, Dan Junwei, Cao Li people's squid viscera protein digestion techniques Research [J] food industry science and technology, 2004,25 (9):83-85.).In addition, correlative study shows, simple organic fertilizer is The needs of practical application can not be met, adding the beneficial microbes such as bacillus, nitrogen-fixing bacteria and phosphorus-solubilizing bacteria in fertilizer can press down Indissoluble nutrient in fabrication pathogen, dissolving soil, produce plant growth regulatory factor etc. play a great role (Chen Qian, The research of several functional microorganisms and application scenario [J] .2010. in biological organic fertilizers send out in Zhang Xinxiong, Zhao Hai, government authorities).Therefore, If being amino acid by the protein efficient degradation in squid viscera, and it is inoculated with and breeds beneficial microbe, for high-valued profit With squid viscera and reduce environmental pollution significant.However, so far, it yet there are no given birth to using squid viscera both at home and abroad Produce the report in terms of liquid biological organic fertilizer.
The content of the invention
It is an object of the invention to overcome the shortcomings of the prior art, and one kind is provided and utilizes squid processing fent, It can not only turn waste into wealth, can be with environmental protection, simple production process, easily operated, life not high to place and equipment requirement The liquid biological organic fertilizer of production contains abundant amino acid and beneficial microbe, can promote the growth of crop and produce the product of fruit Matter, there is the method that liquid biological organic fertilizer is produced using squid viscera of fine promotional value.
It is as follows that the present invention solves the technical scheme that technical problem uses：One kind is organic using squid viscera production liquid biological The method of fertilizer, this method comprise the following steps：
A) it is beaten：Squid viscera is beaten homogeneous, pours into fermentation vat；
B) water is added：Add water into fermentation vat, stir；
C) digest：It is 25-30 DEG C first to control fermentation vat temperature, adds neutral proteinase, stirs, and digests 2-3 hours, 40-45 DEG C of the temperature of fermentation vat is controlled again, neutral lipase is added, stirs, and continues to digest 3-5 hours；
D) sugaring：The sugar relative to squid viscera weight 0.5-1% in step (1) is added into fermentation vat, is stirred；
E) bacterium is added：S. cervisiae, bacillus subtilis, Li's Trichoderma and azotobacter chroococcum are added into fermentation vat, Stir；
F) ferment：Heat-preservation fermentation；
G) stand：Stop stirring, stand 36-48 hours；
H) filter：Floaters on surface is removed, takes supernatant, double gauze filtering；
I) pack：Filtrate is left and taken, is packed.
As preferred：In described step b), the addition of the water is relative to squid viscera weight in step a) 2.0-2.5L/kg.
In described step c), the addition of the neutral proteinase is 400- relative to squid viscera weight in step a) 600U/g；The addition of the neutral lipase is 800-1000U/g relative to squid viscera weight in step a)；
In described step d), the sugar is maltose, fructose, xylose, ribose, deoxyribose, glucose, lactose, half The combination of any one or more in lactose, arabinose.
In described step e), the addition of the S. cervisiae relative to squid viscera weight in step a) be 1 × 108-1×1010CFU/g；The addition of the bacillus subtilis is 1 × 10 relative to squid viscera weight in step a)9-1 ×1011CFU/g；The addition of the Li's Trichoderma is 1 × 10 relative to squid viscera weight in step a)8-1× 1010CFU/g；The addition of the azotobacter chroococcum is 1 × 10 relative to squid viscera weight in step a)8-1×1010CFU/ g。
In described step f), the heat-preservation fermentation condition is：It is 35-45 DEG C to control fermentation vat temperature, fermentation time 18- 20 days, it is passed through sterile filtered air.
The successively method by digesting and beneficial microbe ferments of the invention, makes full use of squid viscera to produce liquid biological Organic fertilizer, be advantageous to improve the profit of squid processing enterprise, and environmental protection.
Beneficial effects of the present invention include the following aspects：
1) raw material that the present invention utilizes is squid processing fent, can not only be turned waste into wealth, can be with environmental protection；This Squid viscera can be almost fully utilized in invention, do not produce secondary pollution；
2) simple production process of the present invention, it is easily operated, it is also not high to place and equipment requirement；
3) present invention, can be using efficient-decomposition protein as amino acid by enzymolysis and microbial fermentation double action；
4) liquid biological organic fertilizer produced by the invention, containing abundant amino acid and beneficial microbe, can promote to make The growth of thing and the quality for producing fruit, have good promotional value.
Specific implementation method
The present invention is described in further detail with reference to embodiments.One kind of the present invention utilizes squid viscera The method for producing liquid biological organic fertilizer, this method comprise the following steps：
A) it is beaten：Squid viscera is beaten homogeneous, pours into fermentation vat；
B) water is added：Add water into fermentation vat, stir；
C) digest：It is 25-30 DEG C first to control fermentation vat temperature, adds neutral proteinase, stirs, and digests 2-3 hours, 40-45 DEG C of the temperature of fermentation vat is controlled again, neutral lipase is added, stirs, and continues to digest 3-5 hours；
D) sugaring：The sugar relative to squid viscera weight 0.5-1% in step (1) is added into fermentation vat, is stirred；
E) bacterium is added：S. cervisiae, bacillus subtilis, Li's Trichoderma and azotobacter chroococcum are added into fermentation vat, Stir；
F) ferment：Heat-preservation fermentation；
G) stand：Stop stirring, stand 36-48 hours；
H) filter：Floaters on surface is removed, takes supernatant, double gauze filtering；
I) pack：Filtrate is left and taken, is packed.
The preferred embodiment of the invention is：
In described step b), the addition of the water is 2.0-2.5L/kg. relative to squid viscera weight in step a)
In described step c), the addition of the neutral proteinase is 400- relative to squid viscera weight in step a) 600U/g；The addition of the neutral lipase is 800-1000U/g relative to squid viscera weight in step a)；
In described step d), the sugar is maltose, fructose, xylose, ribose, deoxyribose, glucose, lactose, half The combination of any one or more in lactose, arabinose.
In described step e), the addition of the S. cervisiae relative to squid viscera weight in step a) be 1 × 108-1×1010CFU/g；The addition of the bacillus subtilis is 1 × 10 relative to squid viscera weight in step a)9-1 ×1011CFU/g；The addition of the Li's Trichoderma is 1 × 10 relative to squid viscera weight in step a)8-1× 1010CFU/g；The addition of the azotobacter chroococcum is 1 × 10 relative to squid viscera weight in step a)8-1×1010CFU/ g。
In described step f), the heat-preservation fermentation condition is：It is 35-45 DEG C to control fermentation vat temperature, fermentation time 18- 20 days, it is passed through sterile filtered air.
Embodiment 1
(1) it is beaten：Leftover bits and pieces-squid viscera of squid processing enterprise is collected, is weighed, homogenizer is used in 12h by squid Internal organ are beaten homogeneous, and pour into fermentation vat.
(2) water is added：Into fermentation vat plus water, the addition of water is 2.0L/ relative to squid viscera weight in step (1) Kg, stir.
(3) digest：It is about 25 DEG C first to control fermentation vat temperature, adds neutral proteinase, addition is relative in step (1) Squid viscera weight is 420U/g, is stirred, and digests 3h；The temperature for controlling fermentation vat again is about 40 DEG C, adds neutral fat Enzyme, addition are 880U/g relative to squid viscera weight in step (1), are stirred, and continue to digest 5h.
(4) sugaring：The glucose relative to squid viscera weight 1% in step (1) is added into fermentation vat, stirring is equal It is even.
(5) bacterium is added：S. cervisiae is added into fermentation vat, addition is relative to squid viscera weight in step (1) 2.5×109CFU/g；Bacillus subtilis is added, addition is 4.1 × 10 relative to squid viscera weight in step (1)9CFU/ g；Li's Trichoderma is added, addition is 1.2 × 10 relative to squid viscera weight in step (1)9CFU/g；It is brown solid to add circle Nitrogen bacterium, addition are 7.1 × 10 relative to squid viscera weight in step (1)8CFU/g, stir.
(6) ferment：It is about 35 DEG C to control fermentation vat temperature, ferments 20 days, is passed through sterile filtered air, heat-preservation fermentation.
(7) stand：Stop stirring, stand 36h.
(8) filter：Floaters on surface is removed, takes supernatant, double gauze filtering.
(9) pack：Filtrate is left and taken, is packed.
Embodiment 2
(1) it is beaten：Leftover bits and pieces-squid viscera of squid processing enterprise is collected, is weighed, homogenizer is used in 12h by squid Internal organ are beaten homogeneous, and pour into fermentation vat.
(2) water is added：Into fermentation vat plus water, the addition of water is 2.5L/ relative to squid viscera weight in step (1) Kg, stir.
(3) digest：It is 30 DEG C first to control fermentation vat temperature, adds neutral proteinase, addition is relative to squid in step (1) Fish guts weight is 570U/g, is stirred, and digests 2h；The temperature 45 C of fermentation vat is controlled again, adds neutral lipase, addition Amount is 940U/g relative to squid viscera weight in step (1), is stirred, and continues to digest 3h.
(4) sugaring：The glucose and 0.5% relative to squid viscera weight 0.5% in step (1) is added into fermentation vat Xylose, stir.
(5) bacterium is added：Add S. cervisiae, addition relative to squid viscera weight in step (1) be 1.7 × 108CFU/g；Bacillus subtilis is added, addition is 2.2 × 10 relative to squid viscera weight in step (1)10CFU/g；Add Enter Li's Trichoderma, addition is 1.8 × 10 relative to squid viscera weight in step (1)9CFU/g；Add azotobacter chroococcum, Addition is 3.0 × 10 relative to squid viscera weight in step (1)9CFU/g, stir.
(6) ferment：It is about 45 DEG C to control fermentation vat temperature, ferments 18 days, is passed through sterile filtered air, heat-preservation fermentation.
(7) stand：Stop stirring, stand 48h.
(8) filter：Floaters on surface is removed, takes supernatant, double gauze filtering.
(9) pack：Filtrate is left and taken, is packed.
The composition and application effect of liquid biological organic fertilizer of the present invention are analyzed below：
1. free amino acid forms：
2.00mL samples are taken, with 15mL 5%HClO4Homogeneous 10min after mixing, 3h is shaken, then uses 5%HClO again4It is fixed To hold 25mL, filter paper filtering, be measured using automatic amino acid analyzer, amino acid content result is represented with mg/100mL, As a result it is as shown in table 1.
The comparison that the raw material of table 1 forms with free amino acid in liquid biological organic fertilizer
As shown in Table 1, the free amino acid of embodiment 1 and the liquid biological organic fertilizer of embodiment 2 composition is significantly larger than former Material, illustrate the enzymolysis in the present invention and microbial fermentation scheme can decompose a large amount of free ammonias of protein generation in squid viscera Base acid.
2. total content of microorganisms：
After embodiment 1 and the dilution of the liquid biological organic fertilizer of embodiment 2, observation by light microscope is carried out, it is observed that Substantial amounts of microorganism.The liquid biological organic fertilizer sample of 0.1mL difference dilution factors is further sampled, using PDA plate cultivation It is coated culture, the results showed that, the average bacteriological sum in liquid biological organic fertilizer reaches 4.45 × 109CFU/mL。
3. practical application effect：
By taking strawberry as an example, the influence of the liquid biological organic fertilizer (embodiment 2 is prepared) to strawberry quality is evaluated.
Experimental program：In same strawberry greenhouse, the strawberry of the random selection swollen fruiting period in 6 ridges is research object.Wherein, 3 For ridge strawberry as control, fertilising is outer by convention, also sprays 2 clear water；Remaining 3 ridge culture is treatment group, and fertilising is outer by convention, Spray liquid biological organic fertilizer in embodiment 1 and dilute 500 times of later dilutions 2 times.After fruit maturation, won at random per ridge Strawberry 20, every group of totally 60 strawberries, carries out the measure of index of correlation, and average.
Fruit is adopted and weighed with scale again；Soluble solid content is determined with hand-held saccharous detector；Soluble sugar content uses anthracene Ketone colorimetric method for determining；Organic acid content is determined by titratable acidity；Vitamin C content uses 2,6- dichloropheno-lindophenol titrations Measure.Concrete outcome is as shown in table 2.
Table 2 sprays influence of the liquid biological organic fertilizer to strawberry quality
As shown in Table 2, in swollen fruiting period sprinkling 500 times of the liquid biological organic fertilizer dilution 2 times of strawberry, ripe grass can be improved Single fruit weight, soluble solid content, soluble sugar content and the Vitamin C content of the certain kind of berries, illustrate that the sprinkling present invention utilizes squid Liquid biological organic fertilizer prepared by internal organ, can improve the quality of strawberry, have preferable application and popularization value.
It should be appreciated that the purposes of above example is merely to illustrate the present invention and is not intended to the protection model of the limitation present invention Enclose.In addition, it should also be understood that, after the technology contents of the present invention have been read, those skilled in the art can make each to the present invention Kind change, modification and/or variation, all these equivalent form of values equally fall within the guarantor that the application appended claims are limited Within the scope of shield.

Claims (2)

  1. A kind of 1. method that liquid biological organic fertilizer is produced using squid viscera, it is characterised in that this method comprises the following steps：
    a）Mashing：Squid viscera is beaten homogeneous, pours into fermentation vat；
    b）Add water：Add water into fermentation vat, stir；
    c）Enzymolysis：It is 25-30 DEG C first to control fermentation vat temperature, adds neutral proteinase, stirs, and digests 2-3 hours, then control 40-45 DEG C of the temperature of fermentation vat processed, neutral lipase is added, is stirred, continue to digest 3-5 hours；
    d）Sugaring：Added into fermentation vat relative to step（1）Middle squid viscera weight 0.5-1% sugar, stirs；
    e）Add bacterium：S. cervisiae, bacillus subtilis, Li's Trichoderma and azotobacter chroococcum, stirring are added into fermentation vat Uniformly；
    f）Fermentation：Heat-preservation fermentation；
    g）Stand：Stop stirring, stand 36-48 hours；
    h）Filtering：Floaters on surface is removed, takes supernatant, double gauze filtering；
    i）Packaging：Filtrate is left and taken, is packed.
  2. 2. the method according to claim 1 that liquid biological organic fertilizer is produced using squid viscera, it is characterised in that：
    Described step b）In, the addition of the water is relative to step a）Middle squid viscera weight is 2.0-2.5L/kg.
    Described step c）In, the addition of the neutral proteinase is relative to step a）Middle squid viscera weight is 400- 600U/g；The addition of the neutral lipase is relative to step a）Middle squid viscera weight is 800-1000U/g；
    Described step d）In, it is described sugar for maltose, fructose, xylose, ribose, deoxyribose, glucose, lactose, galactolipin, The combination of any one or more in arabinose；
    Described step e）In, the addition of the S. cervisiae is relative to step a）Middle squid viscera weight is 1 × 108-1 ×1010CFU/g；The addition of the bacillus subtilis is relative to step a）Middle squid viscera weight is 1 × 109-1× 1011CFU/g；The addition of the Li's Trichoderma is relative to step a）Middle squid viscera weight is 1 × 108-1×1010CFU/ g；The addition of the azotobacter chroococcum is relative to step a）Middle squid viscera weight is 1 × 108-1×1010CFU/g；
    Described step f）In, the heat-preservation fermentation condition is：It is 35-45 DEG C to control fermentation vat temperature, fermentation time 18-20 My god, it is passed through sterile filtered air.
CN201710866859.4A 2017-09-22 2017-09-22 A kind of method that liquid biological organic fertilizer is produced using squid viscera Pending CN107628833A (en)

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN108850686A (en) * 2018-06-09 2018-11-23 浙江亿丰海洋生物制品有限公司 A kind of preparation method of the enzymatic hydrolysis and fermentation squid liver paste for fishes and shrimps opening material
CN113956112A (en) * 2021-12-04 2022-01-21 北海宝农农业科技有限公司 Method for producing full water-soluble microbial fertilizer by using aquatic product processing leftovers
CN113999068A (en) * 2021-11-26 2022-02-01 北海宝农农业科技有限公司 Method for producing full water-soluble sugarcane yield-increasing and sugar-increasing fertilizer by using marine wastes

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CN105695546A (en) * 2016-03-09 2016-06-22 广东海洋大学 Method for preparing liquid organic fertilizer through fermentation of aquatic product waste

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KR100965936B1 (en) * 2009-10-01 2010-06-24 이주일 Preparation of liquefied fertilizer using waste from animal and plant
CN105695546A (en) * 2016-03-09 2016-06-22 广东海洋大学 Method for preparing liquid organic fertilizer through fermentation of aquatic product waste

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108850686A (en) * 2018-06-09 2018-11-23 浙江亿丰海洋生物制品有限公司 A kind of preparation method of the enzymatic hydrolysis and fermentation squid liver paste for fishes and shrimps opening material
CN113999068A (en) * 2021-11-26 2022-02-01 北海宝农农业科技有限公司 Method for producing full water-soluble sugarcane yield-increasing and sugar-increasing fertilizer by using marine wastes
CN113956112A (en) * 2021-12-04 2022-01-21 北海宝农农业科技有限公司 Method for producing full water-soluble microbial fertilizer by using aquatic product processing leftovers

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