CN107619792B - A kind of edible fungus species purification and rejuvenation technology - Google Patents
A kind of edible fungus species purification and rejuvenation technology Download PDFInfo
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- CN107619792B CN107619792B CN201711008570.5A CN201711008570A CN107619792B CN 107619792 B CN107619792 B CN 107619792B CN 201711008570 A CN201711008570 A CN 201711008570A CN 107619792 B CN107619792 B CN 107619792B
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- 241000233866 Fungi Species 0.000 title claims abstract description 24
- 230000003716 rejuvenation Effects 0.000 title claims abstract description 21
- 238000000746 purification Methods 0.000 title claims abstract description 11
- 238000005516 engineering process Methods 0.000 title abstract description 7
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 239000002609 medium Substances 0.000 claims abstract description 26
- 238000012807 shake-flask culturing Methods 0.000 claims abstract description 23
- 238000005520 cutting process Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 17
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229930182555 Penicillin Natural products 0.000 claims abstract description 11
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims abstract description 11
- 229940049954 penicillin Drugs 0.000 claims abstract description 11
- 238000012546 transfer Methods 0.000 claims abstract description 10
- 230000007850 degeneration Effects 0.000 claims abstract description 9
- 235000012255 calcium oxide Nutrition 0.000 claims abstract description 7
- 239000000292 calcium oxide Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 5
- 235000003956 Luffa Nutrition 0.000 claims description 5
- 244000050983 Luffa operculata Species 0.000 claims description 5
- 241000276489 Merlangius merlangus Species 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 230000003115 biocidal effect Effects 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 4
- 244000236655 Diospyros kaki Species 0.000 claims description 3
- 229910052625 palygorskite Inorganic materials 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 244000166124 Eucalyptus globulus Species 0.000 claims description 2
- 230000003068 static effect Effects 0.000 claims description 2
- 235000008597 Diospyros kaki Nutrition 0.000 claims 1
- 230000035899 viability Effects 0.000 abstract description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 7
- 230000007774 longterm Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 235000011511 Diospyros Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000003412 degenerative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of edible fungus species purification and rejuvenation technologies, it includes the following steps, 1 takes the edible fungus species of the degeneration of rejuvenation to be purified to be put into the first culture medium, when the diameter of bacterium colony reaches 2 3cm, cutting tip transposing is placed in the second culture medium, and when the diameter of bacterium colony again reaches 2 3cm, cutting tip transposing is placed in third culture medium, when the diameter of bacterium colony again reaches 2 3cm, cutting tip transposing is placed in the 4th culture medium；2 by the diameter of the strain bacterium colony of last time transposing when reaching 2 3cm, and strain is moved in shake flask culture, penicillin is added in the shake flask culture, strain is in the culture of the liquid level surface of shake flask culture until liquid surface covers with mycelium；3. strain transfer is to alkaline sawdust medium culture to growing mycelium, in alkaline sawdust medium containing mass fraction for 3% 5% quick lime.The present invention can be very good to improve the yield and quality of strain, improve the viability of degenerated strains.
Description
Technical field
The present invention relates to microorganism fungus kind breeding technique field, especially a kind of edible fungus species purification and rejuvenation technology.
Background technology
Edible fungi of china cultivation history is long, and Edible Fungi and outlet rank first in the world.At present, the agriculture in China 80%
There is mushroom industry project in industry region, and important role is carry in rural industriesization adjustment.Edible fungus species are as hair
The basic means of production of edible mushroom are opened up, key effect is played to Edible Fungi, directly affects yield and quality.However, food
In long term storage and turn phenomena such as generally existing production performance for during deteriorates, kind property fails with bacterium strain.Such as oyster mushroom is worked as
Family's kind 2026,89,2028, good No. 1 of section etc. and mushroom govern-house-variety 808,939, L26, L66 etc., these improved seeds exist
Long term storage and during turning to be commissioned to train and supporting, strain quality is gradually degenerated, since long-term input in science and technology is insufficient, mismanagement, aggravation
There is phenomena such as mycelia growth is slow, resistance weakens, yield declines, mushroom matter is poor, causes greatly to Edible Fungi in degenerative process
Economic loss.
Invention content
The object of the present invention is to provide a kind of edible fungus species purification and rejuvenation technologies, can improve the life of degenerative strain
Power improves bacterium yield and quality.
To achieve the above object, the present invention includes the following steps,
(1) mycelia tip is transferred three times：The edible fungus species of the degeneration of rejuvenation to be purified is taken to be put into the first culture medium,
When the diameter of bacterium colony reaches 2-3cm, cutting tip transposing is placed in the second culture medium, and the diameter of bacterium colony again reaches 2-3cm
When, cutting tip transposing is placed in third culture medium, and when the diameter of bacterium colony again reaches 2-3cm, cutting tip transposing is placed in
In four culture mediums；
(2) antibiotic culture：By step（1）When the diameter of the strain bacterium colony of middle last time transposing reaches 2-3cm, by bacterium
Kind move in shake flask culture, add in penicillin in the shake flask culture, the penicillin add for 2,000,000 units/
L, strain is in the culture of the liquid level surface of shake flask culture until liquid surface covers with mycelium；
(3) alkaline sawdust medium culture：By step（2）The strain transfer of culture is straight to alkaline sawdust medium culture
To mycelium is grown, contain the quick lime that mass fraction is 3%-5% in the alkalinity sawdust medium.
Further, step（1）In, temperature of the strain in each culture medium is 30-32 DEG C.
Further, step（2）In, strain is in the liquid level surface static gas wave refrigerator of shake flask culture.
Further, step（3）In, the quick lime that mass fraction is 4% is contained in alkaline sawdust medium.
Further, the sawdust medium further includes the component of following parts by weight, 60-70 parts of sawdust, luffa 5-8
Part, 5-10 parts of oyster shell whiting, 1-3 parts of sucrose, 25-35 parts of wheat bran, 3-5 parts of palygorskite powder.
Further, the sawdust is persimmon sawdust, Chinese bandoline wood sawdust or eucalyptus sawdust.
Good effect of the present invention is as follows：
（1）The present invention can be very good to improve the yield and quality of strain, improve the viability of degenerated strains.
（2）Preparation method of the present invention is easy, and without complex device and process conditions, strong operability is promoted convenient for peasant household.
（3）The present invention is applied widely, applicable to most of edible mushroom.
Specific embodiment
It will make further narration in detail to the embodiment of the present invention below.
Embodiment 1
The purification and rejuvenation of mushroom strain, include the following steps,
（1）It transfers three times at mycelia tip：The edible fungus species of the degeneration of rejuvenation to be purified is taken to be put into the first culture medium, when
When the diameter of bacterium colony reaches 2-3cm, cutting tip transposing is placed in the second culture medium, when the diameter of bacterium colony again reaches 2-3cm,
Cutting tip transposing is placed in third culture medium, and when the diameter of bacterium colony again reaches 2-3cm, cutting tip transposing is placed in the 4th training
It supports in base；The culture medium is conventional medium.Step（1）In, temperature of the strain in each culture medium is 31 DEG C.
（2）Antibiotic culture：By step（1）When the diameter of the strain bacterium colony of middle last time transposing reaches 2-3cm, by bacterium
Kind move in shake flask culture, add in penicillin in the shake flask culture, the penicillin add for 2,000,000 units/
L, strain is in the culture of the liquid level surface of shake flask culture until liquid surface covers with mycelium, and strain is in the liquid level of shake flask culture
Rest culture, is not stirred, and can prevent most bacterial viruses in this way from surviving, and retains target strain.
（3）Alkaline sawdust medium culture：By step（1）The strain transfer of culture is straight to alkaline sawdust medium culture
To mycelium is grown, contain the quick lime that mass fraction is 4% in the alkalinity sawdust medium.The sawdust medium also wraps
Include the component of following parts by weight, 65 parts of persimmon sawdust, 7 parts of luffa, 8 parts of oyster shell whiting, 2 parts of sucrose, 30 parts of wheat bran, palygorskite powder 4
Part.
（4）By step（3）It turns out the fructification come and carries out tissue separation, obtained strain is virus-free strain.
Control group 1
The edible fungus species of the degeneration of the rejuvenation to be purified of embodiment 1 are chosen, workshop rule are carried out according to conventional squamous subculture
Modelling produces.
Embodiment 2
The purification and rejuvenation of flat mushroom strain
（1）It transfers three times at mycelia tip：The edible fungus species of the degeneration of rejuvenation to be purified is taken to be put into the first culture medium, when
When the diameter of bacterium colony reaches 2-3cm, cutting tip transposing is placed in the second culture medium, when the diameter of bacterium colony again reaches 2-3cm,
Cutting tip transposing is placed in third culture medium, and when the diameter of bacterium colony again reaches 2-3cm, cutting tip transposing is placed in the 4th training
It supports in base；The culture medium is conventional medium.Step（1）In, temperature of the strain in each culture medium is 30 DEG C.
（2）Antibiotic culture：By step（1）When the diameter of the strain bacterium colony of middle last time transposing reaches 2-3cm, by bacterium
Kind move in shake flask culture, add in penicillin in the shake flask culture, the penicillin add for 2,000,000 units/
L, strain is in the culture of the liquid level surface of shake flask culture until liquid surface covers with mycelium, and strain is in the liquid level of shake flask culture
Rest culture, is not stirred；
（3）Alkaline sawdust medium culture：By step（1）The strain transfer of culture is straight to alkaline sawdust medium culture
To mycelium is grown, contain the quick lime that mass fraction is 5% in the alkalinity sawdust medium.The sawdust medium also wraps
Include the component of following parts by weight, 60 parts of Chinese bandoline wood sawdust, 8 parts of luffa, 10 parts of oyster shell whiting, 1 part of sucrose, 35 parts of wheat bran, slope thread
3 parts of mountain flour.
（4）By step（3）It turns out the fructification come and carries out tissue separation, obtained strain is virus-free strain.
Control group 2
The edible fungus species of the degeneration of the rejuvenation to be purified of embodiment 2 are chosen, workshop rule are carried out according to conventional squamous subculture
Modelling produces.
The purification and rejuvenation of 3 Uricularia polytricha strain of embodiment
（1）It transfers three times at mycelia tip：The edible fungus species of the degeneration of rejuvenation to be purified is taken to be put into the first culture medium, when
When the diameter of bacterium colony reaches 2-3cm, cutting tip transposing is placed in the second culture medium, when the diameter of bacterium colony again reaches 2-3cm,
Cutting tip transposing is placed in third culture medium, and when the diameter of bacterium colony again reaches 2-3cm, cutting tip transposing is placed in the 4th training
It supports in base；The culture medium is conventional medium.Step（1）In, temperature of the strain in each culture medium is 32 DEG C.
（2）Antibiotic culture：By step（1）When the diameter of the strain bacterium colony of middle last time transposing reaches 2-3cm, by bacterium
Kind move in shake flask culture, add in penicillin in the shake flask culture, the penicillin add for 2,000,000 units/
L, strain is in the culture of the liquid level surface of shake flask culture until liquid surface covers with mycelium, and strain is in the liquid level of shake flask culture
Rest culture, is not stirred；
（3）Alkaline sawdust medium culture：By step（1）The strain transfer of culture is straight to alkaline sawdust medium culture
To mycelium is grown, contain the quick lime that mass fraction is 3% in the alkalinity sawdust medium.The sawdust medium also wraps
Include the component of following parts by weight, 70 parts of Chinese bandoline wood sawdust, 5 parts of luffa, 5 parts of oyster shell whiting, 3 parts of sucrose, 25 parts of wheat bran, slope thread
5 parts of mountain flour.
（4）By step（3）It turns out the fructification come and carries out tissue separation, obtained strain is virus-free strain.
Control group 3
The edible fungus species of the degeneration of the rejuvenation to be purified of embodiment 3 are chosen, workshop rule are carried out according to conventional squamous subculture
Modelling produces.
By testing, after embodiment 1,2,3 compares respectively with comparative example 1,2,3, it is found that compared to strain conventional treatment side
Method, the present embodiment strain is after squamous subculture, and yield higher, more stable, resistance is strong, the biological transformation ratio ＞ 5% of production,
Pollute scrappage ＜ 5%, far superior to processed conventionally comparative example group.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications of these embodiments will be apparent for those skilled in the art, it is as defined herein
General Principle can in other embodiments be realized in the case of the spirit or scope for not departing from patent of the present invention.Therefore, originally
Invention is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein
Consistent most wide range.
Claims (4)
- A kind of method 1. edible fungus species are purificated and rejuvenated, it is characterised in that：Include the following steps,（1）It transfers three times at mycelia tip：The edible fungus species of the degeneration of rejuvenation to be purified is taken to be put into the first culture medium, work as bacterium colony Diameter when reaching 2-3cm, cutting tip transposing is placed in the second culture medium, when the diameter of bacterium colony again reaches 2-3cm, cutting Tip transposing is placed in third culture medium, and when the diameter of bacterium colony again reaches 2-3cm, cutting tip transposing is placed in the 4th culture medium In；（2）Antibiotic culture：By step（1）When the diameter of the strain bacterium colony of middle last time transposing reaches 2-3cm, strain is moved Into shake flask culture, penicillin is added in the shake flask culture, the addition of the penicillin is 2,000,000 units/L, bacterium Kind is in the culture of the liquid level surface of shake flask culture until liquid surface covers with mycelium；（3）Alkaline sawdust medium culture：By step（2）The strain transfer of culture to alkaline sawdust medium culture until Mycelium is grown, contains the quick lime that mass fraction is 4% in the alkalinity sawdust medium；The alkalinity sawdust medium is also Include the component of following parts by weight, 60-70 parts of sawdust, 5-8 parts of luffa, 5-10 parts of oyster shell whiting, 1-3 parts of sucrose, wheat bran 25-35 Part, 3-5 parts of palygorskite powder.
- 2. a kind of edible fungus species purification and rejuvenation method according to claim 1, it is characterised in that：Step（1）In, strain Temperature in each culture medium is 30-32 DEG C.
- 3. a kind of edible fungus species purification and rejuvenation method according to claim 1, it is characterised in that：Step（2）In, strain In the liquid level surface static gas wave refrigerator of shake flask culture.
- 4. a kind of edible fungus species purification and rejuvenation method according to claim 1, it is characterised in that：The sawdust is kaki Bits, Chinese bandoline wood sawdust or eucalyptus sawdust.
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| CN201711008570.5A CN107619792B (en) | 2017-10-25 | 2017-10-25 | A kind of edible fungus species purification and rejuvenation technology |
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| CN102907258A (en) * | 2012-11-13 | 2013-02-06 | 四川省青川县川珍实业有限公司 | Detoxification method for edible fungus strain |
| CN104488557B (en) * | 2014-12-26 | 2016-09-14 | 华盛绿能农业科技有限公司 | A kind of method of edible fungus species purification and rejuvenation |
| CN105219652B (en) * | 2015-09-17 | 2018-02-23 | 上海市农业科学院 | A kind of isolation and purification method of wild Bachu mushroom mycelium |
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Address after: No. 164, middle Huayang Road, Zhuozhou City, Baoding City, Hebei Province 072750 Patentee after: Xiangtian Technology Co.,Ltd. Address before: No. 164, middle Huayang Road, Zhuozhou City, Baoding City, Hebei Province 072750 Patentee before: XIANGTIAN AGRICULTURAL DEVELOPMENT GROUP Co.,Ltd. |
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Denomination of invention: A Purification and Revitalization Technology for Edible Fungi Species Effective date of registration: 20230425 Granted publication date: 20180706 Pledgee: Hebei Zhuozhou Rural Commercial Bank Co.,Ltd. Baichigan Branch Pledgor: Xiangtian Technology Co.,Ltd. Registration number: Y2023980039254 |
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Granted publication date: 20180706 Pledgee: Hebei Zhuozhou Rural Commercial Bank Co.,Ltd. Baichigan Branch Pledgor: Xiangtian Technology Co.,Ltd. Registration number: Y2023980039254 |
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Denomination of invention: A purification and rejuvenation technique for edible mushroom strains Granted publication date: 20180706 Pledgee: Hebei Zhuozhou Rural Commercial Bank Co.,Ltd. Baichigan Branch Pledgor: Xiangtian Technology Co.,Ltd. Registration number: Y2024980014321 |