CN107619786A - A kind of removable culture plate - Google Patents
A kind of removable culture plate Download PDFInfo
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- CN107619786A CN107619786A CN201710861598.7A CN201710861598A CN107619786A CN 107619786 A CN107619786 A CN 107619786A CN 201710861598 A CN201710861598 A CN 201710861598A CN 107619786 A CN107619786 A CN 107619786A
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- culture
- culture plate
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- sheet frame
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Abstract
The invention discloses a kind of removable culture plate, is made up of culture sheet frame, several bar shaped culture holes, culture plate lid;The inframe for cultivating sheet frame is provided with the divider of crisscross setting, and each divider forms the grid hole for fixing bar shaped culture hole;Each bar shaped culture hole is removably mounted in latticed;Culture plate lid lid is located on culture sheet frame.The application is removable culture plate, culture sheet frame is arranged to detachable with culture plate lid, so culture sheet frame can reuse with culture plate lid, and sufficient amount of culture plate can be selected to use as needed, reduce the usage amount of culture plate, cost is saved, not only avoid the problem of utilization rate is low, and improve the speed of experimental implementation.
Description
Technical field
The present invention relates to a kind of cell culture vessel, more particularly to a kind of removable culture plate.
Background technology
In INVENTIONModern cell culture technique, it is often necessary to which large-scale culture cell is, it is necessary to which substantial amounts of Tissue Culture Plate is carried out
Detection and analysis.After the 1990s, SBS is unified to the apparent size of Tissue Culture Plate, now all of detection point
Analyzer device is by the fixed test frame of SBS standard sizes design.And in biological study field and medical diagnosis experiment, no matter
It is microculture or cell culture, is also directed to various culture holders, wherein 96 well culture plates is molecule
The conventional culture apparatus tested and analyzed in biological experiment.96 well culture plates are in blood clotting and hemagglutination-inhibition test, molecular biosciences
Learn and be widely used in the experiment such as dilution, cell culture, ELISA Plate.
But there is following defect in existing 96 well culture plate:
(1) hole count of 96 well culture plate of the prior art is more, and is not identified significantly between Kong Yukong, easily makes
Row and column into experimental implementation process mesopore is not easy to be identified, in the case that particularly institute's reagent adding is shown without color, due to
The ranks in hole are obscured, occur it is wrong plus or leakage sample-adding product phenomenon, influence the accuracy of experimental result, cause the failure of an experiment and when
Between, the waste of consumptive material.
(2) 96 well culture plate of the prior art, the hole and base for cultivating cell are fixed, and after experiment terminates, are used
Whole culture plate be all not available for secondary use, so as to cause to waste.And most of orifice plate does not meet SBS requirements, often makes
It is used for automated laboratory instrument into many problems of microplate.
(3) 96 well culture plate of the prior art is on culture cell, if after certain hole cell is contaminated, general justifying cell
It can not utilize, so as to cause the time, the waste of reagent.
(4) culture plate of the prior art, cultivate cell hole and base fix it is non-dismountable, can not measure as needed from
By assembling, the less laboratory of sample size using when can cause the waste of plate hole resource.
(5) all plate holes of culture plate of the prior art are all fixed on sheet frame, when being loaded in each plate hole
The sample of addition certain volume is needed, causes the waste of some rare samples, and detection time extends.
The content of the invention
For overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of removable culture plate.The application
For removable culture plate, culture sheet frame is arranged to detachable with culture plate lid, so sheet frame is cultivated and culture plate lid all may be used
With recycling, and sufficient amount of culture plate can be selected to use as needed, reduce the usage amount of culture plate, save into
This, not only avoid the problem of utilization rate is low, and improve the speed of experimental implementation.
The purpose of the present invention adopts the following technical scheme that realization:A kind of removable culture plate, by culture sheet frame, several
Bar shaped culture hole, culture plate lid composition;The inframe of the culture sheet frame is provided with the divider of crisscross setting, each divider
Form the grid hole for fixing bar shaped culture hole;Each bar shaped culture hole is removably mounted in latticed;It is described
Culture plate lid lid is located on culture sheet frame.
Further, the framework upper edge of the culture sheet frame and left margin distributing labels have Roman number and English words
Mother, the specific coordinate of culture hole is determined for marking;The grid hole corresponds with the position of Roman number and English alphabet;
The culture plate upper frame edge edge and the intersection of left margin are provided with the first unfilled corner for being used for determining culture plate direction;The culture plate
The upper edge of lid is provided with the second unfilled corner being adapted with cultivating the first unfilled corner of sheet frame with left margin intersection.
Further, the culture sheet frame is surrounded by for supporting culture plate lid to increase ventilative lug boss;Institute
Stating culture plate lid includes panel and is arranged on the surrounding edge of panel surrounding;The culture plate interior surface is provided with crisscross setting
Raised line is ventilative to increase for support panel;The raised line is connected on the divider.
Further, the upper edge of the frame side wall of the culture sheet frame and lower edge, which are equipped with, is easy to hand-held culture plate
Skid resistance strip.
Further, the left margin of the frame side wall of the culture sheet frame and the right edge are equipped with for culture plate to be adapted to
The positioning hole being fixed on automatic detection instrument.
Further, the framework bottom of the culture sheet frame is provided with the groove for being used for fixing ranked and stacked pile culture plate;It is described
Culture plate cap upper surface is provided with the sunk structure being adapted with the groove shapes.
Further, the length of the culture sheet frame is 127.76 ± 0.05mm, and width is 85.48 ± 0.05mm, thickness
For 14.35 ± 0.5mm;The height of the grid hole is 5.0 ± 0.05mm;The upper surface of grid hole to the upper surface of culture sheet frame
Height be 4.6 ± 0.05mm, and the height of bar shaped culture hole and the difference in height of culture sheet frame are also 5.0 ± 0.05mm.
Further, each bar shaped culture hole has 8 culture holes, and culture hole is water white transparency.
Further, the height of the culture hole is 12.6 ± 0.05mm, and internal diameter is 6.87 ± 0.05mm, and pitch of holes is
9.0±0.05mm。
Further, movement and fixed bar shaped culture hole when the both ends of each bar shaped culture hole are provided with easy to operation
Handle, one of handle is provided with fixing hole.
Compared with prior art, the beneficial effects of the present invention are:
The application is removable culture plate, and culture sheet frame is arranged into detachable with culture plate lid, so cultivates sheet frame
It can be reused with culture plate lid, and sufficient amount of culture plate can be selected to use as needed, reduce culture plate
Usage amount, cost is saved, not only avoid the problem of utilization rate is low, and improve the speed of experimental implementation.
Brief description of the drawings
Fig. 1 is the structural representation of the removable culture plate of present pre-ferred embodiments;
Fig. 2 is the disassembly diagram of removable culture plate;
Fig. 3 is the cross-sectional view of removable culture plate.
In figure:1st, sheet frame is cultivated;11st, divider;12nd, grid hole;13rd, Roman number;14th, English alphabet;15th, first lack
Angle;16th, lug boss;17th, skid resistance strip;18th, positioning hole;19th, locating part;110th, groove;2nd, bar shaped culture hole;21st, handle;
22nd, fixing hole;3rd, culture plate lid;31st, the second unfilled corner;32nd, raised line;33rd, sunk structure.
Embodiment
Below, with reference to accompanying drawing and embodiment, the present invention is described further, it is necessary to which explanation is, not
Under the premise of afoul, new implementation can be formed between various embodiments described below or between each technical characteristic in any combination
Example.
As Figure 1-3, a kind of removable culture plate, by culture sheet frame 1, several bar shaped culture holes 2, culture plate lid
3 compositions;
Wherein, the inframe for cultivating sheet frame is provided with the divider 11 of crisscross setting, and divider includes being parallel to each other and phase
Mutually vertical horizontal stripe and vertical bar, each horizontal stripe and vertical bar form the grid hole 12 for fixing bar shaped culture hole;Each bar shaped training
Foster hole is removably mounted in latticed;Culture plate lid lid is located on culture sheet frame.
The application is removable culture plate, and culture sheet frame is arranged into detachable with culture plate lid, so cultivates sheet frame
It can be reused with culture plate lid, and sufficient amount of culture plate can be selected to use as needed, reduce culture plate
Usage amount, cost is saved, not only avoid the problem of utilization rate is low, and improve the speed of experimental implementation.
Preferably, cultivate the framework upper edge of sheet frame and left margin distributing labels have 1~12 Roman number 13
With A~H English alphabet 14, the specific coordinate for mark determination culture hole;Grid hole and Roman number and English alphabet
Position corresponds;Culture plate upper frame edge edge and the intersection of left margin are provided with the first unfilled corner for being used for determining culture plate direction
15;The upper edge of culture plate lid is provided with the second unfilled corner 31 being adapted with cultivating the first unfilled corner of sheet frame with left margin intersection.
By setting Roman number and English alphabet, the second unfilled corner of the first unfilled corner, whole culture plate is set to add a variety of obvious marks
Know, ensure that the accuracy of experimental result.
Preferably, that cultivates sheet frame is surrounded by lug boss 16, and the height of the lug boss is higher than the height at edge,
For supporting culture plate lid, increase permeation functions.Culture plate lid includes panel and is arranged on the surrounding edge of panel surrounding;Culture plate lid
Inner surface is provided with the raised line 32 of crisscross setting, ventilative to increase for support panel;Raised line is connected on divider.
Preferably, cultivate the upper edge of the frame side wall of sheet frame and lower edge is equipped with and is easy to hand-held culture plate
Skid resistance strip 17.
Preferably, the left margin and the right edge for cultivating the frame side wall of sheet frame are equipped with for culture plate to be adapted to
The positioning hole 18 being fixed on automatic detection instrument, left and right each two positioning holes in edge.
Preferably, the framework bottom for cultivating sheet frame is provided with the groove 110 for being used for fixing ranked and stacked pile culture plate;Training
Support plate cap upper surface and be provided with the sunk structure 33 being adapted with groove shapes.
Preferably, the length for cultivating sheet frame is 127.76 ± 0.05mm, and width is 85.48 ± 0.05mm, thickness
For 14.35 ± 0.5mm, the design is the size design in accordance with SBS, can be adapted to automatic detecting instrument all in market
Device, so as to the quick accurate detection and analysis for completing experiment.The height of grid hole is 5.0 ± 0.05mm;The upper surface of grid hole
Height to the upper surface of culture sheet frame be 4.6 ± 0.05mm, and difference in height of the height of bar shaped culture hole with cultivating sheet frame
Also it is 5.0 ± 0.05mm so that culture hole perfectly can be fixed on sheet frame, avoided culture hole from being come off from sheet frame and caused reality
The failure and waste tested.The application culture plate appearance and size meets SBS requirements, and has positioning hole in sheet frame bottom, can be solid
Due to all automatic detection instruments on instrument, can be adapted to.
Wherein, bar shaped culture hole, strip, each bar shaped culture hole have 8 culture holes, and culture hole is water white transparency, just
In observation sample.Further, the height of culture hole is 12.6 ± 0.05mm, and internal diameter is 6.87 ± 0.05mm, pitch of holes 9.0
±0.05mm.The handle 21 of movement and fixed bar shaped culture hole when the both ends of each bar shaped culture hole are provided with easy to operation, its
In one being provided with fixing hole 22 on hand;Cultivate the locating part 19 being convexly equipped with along side for fixing bar shaped culture hole of sheet frame.
Wherein, culture plate lid is rectangle, is mainly used in preventing culture medium from volatilizing and preventing from polluting.The raised line that culture plate covers
Including three longitudinal raised lines and two horizontal raised lines, Fig. 3 show three longitudinal raised lines, is mainly used in supporting plate lid, in cell
Permeation functions during culture.Culture plate covers the design that edge is provided with the second unfilled corner with left margin intersection, is mutually fitted with culture sheet frame
Match somebody with somebody, for determining culture plate direction.
Above-mentioned embodiment is only the preferred embodiment of the present invention, it is impossible to the scope of protection of the invention is limited with this,
The change and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed scope.
Claims (10)
1. a kind of removable culture plate, it is characterised in that be made up of culture sheet frame, several bar shaped culture holes, culture plate lid;
The inframe of the culture sheet frame is provided with the divider of crisscross setting, and each divider is formed for fixing bar shaped culture hole
Grid hole;Each bar shaped culture hole is removably mounted in latticed;The culture plate lid lid is located on culture sheet frame.
2. culture plate as claimed in claim 1, it is characterised in that the framework upper edge of the culture sheet frame and left margin distribution
Roman number and English alphabet are marked with, the specific coordinate of culture hole is determined for marking;The grid hole and Roman number and
The position of English alphabet corresponds;The culture plate upper frame edge edge and the intersection of left margin, which are provided with, to be used to determine culture plate side
To the first unfilled corner;The upper edge of the culture plate lid is provided with left margin intersection and is adapted with cultivating the first unfilled corner of sheet frame
The second unfilled corner.
3. culture plate as claimed in claim 1, it is characterised in that the culture sheet frame is surrounded by for supporting culture plate
Cover to increase ventilative lug boss;The culture plate lid includes panel and is arranged on the surrounding edge of panel surrounding;The culture plate lid
Inner surface is provided with the raised line of crisscross setting, ventilative to increase for support panel;The raised line is connected to the divider
On.
4. culture plate as claimed in claim 1, it is characterised in that the upper edge of the frame side wall of the culture sheet frame and following
The skid resistance strip for being easy to hand-held culture plate along being equipped with.
5. culture plate as claimed in claim 1, it is characterised in that the left margin of the frame side wall of the culture sheet frame and the right
Along the positioning hole being equipped with for culture plate adaptation to be fixed on automatic detection instrument.
6. culture plate as claimed in claim 1, it is characterised in that the framework bottom of the culture sheet frame, which is provided with, is used for fixed bed
Stack the groove for putting culture plate;The culture plate cap upper surface is provided with the sunk structure being adapted with the groove shapes.
7. culture plate as claimed in claim 1, it is characterised in that the length of the culture sheet frame is 127.76 ± 0.05mm,
Width is 85.48 ± 0.05mm, and thickness is 14.35 ± 0.5mm;The height of the grid hole is 5.0 ± 0.05mm;Grid hole
The height of upper surface to the upper surface of culture sheet frame is 4.6 ± 0.05mm, and the height of bar shaped culture hole is with cultivating sheet frame
Difference in height is also 5.0 ± 0.05mm.
8. culture plate as claimed in claim 1, it is characterised in that each bar shaped culture hole has 8 culture holes, culture hole
For water white transparency.
9. culture plate as claimed in claim 8, it is characterised in that the height of the culture hole is 12.6 ± 0.05mm, internal diameter
For 6.87 ± 0.05mm, pitch of holes is 9.0 ± 0.05mm.
10. culture plate as claimed in claim 1, it is characterised in that the both ends of each bar shaped culture hole, which are provided with, to be easy to grasp
The handle of movement and fixed bar shaped culture hole when making, one of handle are provided with fixing hole.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201710861598.7A CN107619786A (en) | 2017-09-21 | 2017-09-21 | A kind of removable culture plate |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710861598.7A CN107619786A (en) | 2017-09-21 | 2017-09-21 | A kind of removable culture plate |
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Publication Number | Publication Date |
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CN107619786A true CN107619786A (en) | 2018-01-23 |
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ID=61090427
Family Applications (1)
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CN201710861598.7A Pending CN107619786A (en) | 2017-09-21 | 2017-09-21 | A kind of removable culture plate |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109507183A (en) * | 2018-12-25 | 2019-03-22 | 菏泽学院 | A kind of pH self-operated measuring unit and its application method based on pigment instruction |
CN113512494A (en) * | 2021-05-06 | 2021-10-19 | 中国科学院上海高等研究院 | Cell micro-fluidic culture chip |
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CN202486136U (en) * | 2012-04-09 | 2012-10-10 | 无锡国盛精密模具有限公司 | Enzyme-linked immuno-sorbent assay (ELISA) plate shelf |
CN103323496A (en) * | 2013-05-24 | 2013-09-25 | 阮仕荣 | High throughput skin corrosion and absorption detecting boards |
CN204803324U (en) * | 2015-07-02 | 2015-11-25 | 广州洁特生物过滤股份有限公司 | Cell culture board of area embedding ware |
CN204824852U (en) * | 2015-07-17 | 2015-12-02 | 杭州秀川科技有限公司 | Board is cultivateed to airtight bacterium |
CN204964516U (en) * | 2015-09-16 | 2016-01-13 | 广州璞雅医药生物科技有限公司 | Novel ELIAS plate |
CN204989189U (en) * | 2015-06-30 | 2016-01-20 | 广州洁特生物过滤股份有限公司 | Elisa plate |
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2017
- 2017-09-21 CN CN201710861598.7A patent/CN107619786A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN202486136U (en) * | 2012-04-09 | 2012-10-10 | 无锡国盛精密模具有限公司 | Enzyme-linked immuno-sorbent assay (ELISA) plate shelf |
CN103323496A (en) * | 2013-05-24 | 2013-09-25 | 阮仕荣 | High throughput skin corrosion and absorption detecting boards |
CN204989189U (en) * | 2015-06-30 | 2016-01-20 | 广州洁特生物过滤股份有限公司 | Elisa plate |
CN204803324U (en) * | 2015-07-02 | 2015-11-25 | 广州洁特生物过滤股份有限公司 | Cell culture board of area embedding ware |
CN204824852U (en) * | 2015-07-17 | 2015-12-02 | 杭州秀川科技有限公司 | Board is cultivateed to airtight bacterium |
CN204964516U (en) * | 2015-09-16 | 2016-01-13 | 广州璞雅医药生物科技有限公司 | Novel ELIAS plate |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109507183A (en) * | 2018-12-25 | 2019-03-22 | 菏泽学院 | A kind of pH self-operated measuring unit and its application method based on pigment instruction |
CN113512494A (en) * | 2021-05-06 | 2021-10-19 | 中国科学院上海高等研究院 | Cell micro-fluidic culture chip |
CN113512494B (en) * | 2021-05-06 | 2024-03-22 | 中国科学院上海高等研究院 | Cell micro-fluidic culture chip |
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Application publication date: 20180123 |