CN217973151U

CN217973151U - Enzyme-linked immunosorbent assay (ELISA) instrument for organoid culture and detection

Info

Publication number: CN217973151U
Application number: CN202222136826.3U
Authority: CN
Inventors: 胡良昌; 王洋; 武晓丽; 孟子愉; 王军; 贾正虎
Original assignee: New Anji Medical Technology Shenzhen Co ltd
Current assignee: New Anji Medical Technology Shenzhen Co ltd
Priority date: 2022-08-15
Filing date: 2022-08-15
Publication date: 2022-12-06
Anticipated expiration: 2032-08-15

Abstract

The utility model provides an enzyme-labeling instrument for kind organ culture detection relates to enzyme-labeling instrument technical field, including instrument display screen and incubator, the culture tank has evenly been seted up to the positive vertical direction of incubator, the inside culture box that is equipped with of culture tank, culture box top surface block is equipped with sealed lid, the culture box surface evenly is equipped with the culture dish, sealed lid top surface corresponds position department with the culture dish and has seted up the water conservancy diversion hole, sealed lid top surface is equipped with the spacing groove along water conservancy diversion hole edge, sealed lid top surface linear array has seted up spacing straight flute, the inside block of spacing straight flute is equipped with spacing slat, the incubator openly sets up the culture tank and carries out placing of culture box and sealed lid, sealed lid top surface covers through the slip of spacing slat, can take out or place the cell liquid of same row one by one alone and do not influence the environment in other culture dishes, keep not influenced when taking out and placing the culture liquid between the adjacent culture dish, thereby reduce the environmental error between the adjacent culture dish, guarantee the precision that cell culture detected.

Description

Enzyme-linked immunosorbent assay (ELISA) instrument for organoid culture and detection

Technical Field

The utility model relates to a ELIASA technical field especially relates to an ELIASA is used in kind organ culture detection.

Background

According to the metering mechanism based on cell enzyme-labeling instrument that chinese patent number is disclosed for CN213337654U, the embedding of fixed mounting panel top middle part installs down piezoelectric push rod, lower piezoelectric push rod bottom fixedly connected with pushes down the slurcam, the welding of fixed mounting panel bottom bilateral symmetry has the joint fixed plate, the joint is connected with fixedly between two joint fixed plates and places the board, the joint hole has all been seted up on joint fixed plate and the fixed board top of placing, the inboard welding of joint hole has the joint spring, joint spring top spot welding is connected with pushes down the joint post, a plurality of joint mouth has been seted up to fixed board top equidistance of placing, place the syringe through fixed board and the joint mouth of placing, and restrict the position of syringe through pushing spring and radian limiting plate, the condition that the syringe appears the skew when the injection, the stability of injection has been guaranteed, and can once only place a plurality of syringes, conveniently pack the multiunit test tube simultaneously, the efficiency of measurement has been improved.

When injecting or taking out to the cell sap among the above-mentioned patent document, need whole test tube to expose the back and inject one by one or take out, thereby the test tube of waiting for the operation exposes and leads to detecting the data deviation that appears by the influence of environment easily in the air to pollute the cell sap, and other test tubes are not influenced when can't realizing that the test tube takes out, detect and receive the environmental impact easily when taking out and injecting into, are unfavorable for in-service use.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a microplate reader for organ culture detection.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a kind organ culture detects uses ELIASA, includes instrument display screen and incubator, the fixed instrument display screen that is equipped with in incubator top surface one side, the culture tank has evenly been seted up to the positive vertical direction of incubator, the inside culture box that is equipped with of culture tank, culture box top surface block is equipped with sealed lid, the culture box surface evenly is equipped with the culture dish, sealed lid top surface corresponds position department with the culture dish and has seted up the water conservancy diversion hole, sealed lid top surface is equipped with the spacing groove along water conservancy diversion hole edge, and the spacing groove is blind groove, sealed lid top surface linear array has seted up spacing straight flute, the inside block of spacing straight flute is equipped with spacing slat.

Preferably, spacing tongue runs through with the same water conservancy diversion hole that is listed as of sealed lid top surface, spacing slat bottom surface both sides and spacing tongue block, spacing tongue cross-section is the type of falling T, spacing slat normal cross-section is the I shape.

Preferably, cultivate box top surface four corners and sealed lid bottom surface four corners block, cultivate the inside culture box and sealed lid that all is equipped with of groove, cultivate box and sealed lid outside edge all adopt the fillet to handle.

Preferably, one side bolt joint that instrument display screen was kept away from to the incubator top surface is equipped with the storage tank, the incubator top surface is equipped with the regulation and control button, regulation and control button and instrument display screen electric connection.

Preferably, one end of the limiting straight groove penetrates through one side of the top surface of the sealing cover, the width of the limiting strip plate is the same as that of the top surface of the sealing cover, and the width of the limiting strip plate is 3/2 of the diameter of the flow guide hole.

Preferably, the outer edge of the incubator is subjected to round angle smoothing treatment, and the outer edge of the instrument display screen is subjected to round angle smoothing treatment.

Advantageous effects

The utility model discloses in, adopt the incubator openly to set up the culture tank and cultivate placing of box and sealed lid, sealed lid top surface covers through the slip of spacing slat, can take out one by one or place the cell liquid of same row alone and do not influence the environment in other culture dishes, keeps not influenced when taking out and placing the culture solution between the adjacent culture dish to reduce the environmental error between the adjacent culture dish, guarantee the precision that cell culture detected.

Drawings

FIG. 1 is a perspective view of a microplate reader for organoid culture detection;

FIG. 2 is a view showing a stack structure of a cassette of a microplate reader for organoid culture detection;

FIG. 3 is a schematic perspective view of a microplate reader for organoid culture detection;

FIG. 4 is a drawing showing a disassembled structure of a cassette of a microplate reader for organoid culture detection.

Illustration of the drawings:

1. an instrument display screen; 2. a control button; 3. a storage box; 4. an incubator; 5. a culture tank; 6. a culture box; 7. a sealing cover; 8. a culture dish; 9. a flow guide hole; 10. a limiting groove; 11. a limiting batten; 12. and a limiting straight groove.

Detailed Description

In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand, the present invention will be further explained below with reference to the following embodiments and the accompanying drawings, but the following embodiments are only the preferred embodiments of the present invention, and not all embodiments are included. Based on the embodiments in the embodiment, those skilled in the art can obtain other embodiments without making creative efforts, and all of them belong to the protection scope of the present invention.

Specific embodiments of the present invention will be described below with reference to the accompanying drawings.

The specific embodiment is as follows:

referring to fig. 1-4, a class organ culture detects uses ELIASA, including instrument display screen 1 and incubator 4, incubator 4 top surface one side is fixed to be equipped with instrument display screen 1, incubator 4 openly has evenly seted up culture tank 5 in the vertical direction, culture tank 5 is inside to be equipped with culture box 6, culture box 6 top surface block is equipped with sealed lid 7, culture box 6 surface evenly is equipped with culture dish 8, culture dish 8 inside culture box 6 can directly open through sealed lid 7 whole and carry out placing one by one of cell culture liquid, incubator 4 is inside to set up the component that detects the cell, equipment etc., do not belong to the protection domain of this application, to the structure of incubator 4 inside and cell detection principle technical personnel in the field can carry out the inference according to the theory of operation and the structure of ELIASA.

Instrument display screen 1 shows to the cell result of 5 detections of culture tank in incubator 4, one side bolt that instrument display screen 1 was kept away from to 4 top surfaces of incubator is equipped with

storage tank

3, 4 top surfaces of incubator are equipped with regulation and control button 2, regulation and control button 2 and 1 electric connection of instrument display screen, regulation and control button 2 is adjusted each item parameter that used to 4 detections of incubator, be convenient for detect the cell sap accurately, the inside syringe that can place the injection cell sap of storage tank 3, the syringe needle, instrument structures such as stock solution bottle, be convenient for to the injection of cell sap and take out, moreover, the steam generator is simple in structure, and is convenient for take.

The top surface of the sealing cover 7 is provided with a flow guide hole 9 at a position corresponding to the culture dish 8, the top surface of the sealing cover 7 is provided with a limiting groove 10 along the edge of the flow guide hole 9, the limiting groove 10 is a blind groove, the top surface of the sealing cover 7 is provided with a limiting straight groove 12 in a linear array, the limiting straight groove 12 is internally provided with a limiting slat 11 in a clamping way, when the cell culture solution is injected at a single position of the culture dish 8 in the culture box 6 for use, the limiting slat 11 slides and is pulled away along the limiting straight groove 12, so that the flow guide hole 9 at the bottom of the limiting slat 11 is exposed, at the moment, the injector containing the cell solution can be vertically inserted into the flow guide hole 9, so that one end of the injector close to the needle head is abutted against the limiting groove 10, the limiting groove 10 vertically supports the injector, and a worker only needs to push the other end of the injector to push the cell solution into the culture dish 8, in the method can vertically, intuitively and accurately penetrate through the volume of the cell solution pushed into the culture dish 8, the cell sap is injected into the injector quantitatively and then injected into the culture dish 8 for detection, the next culture dish 8 needs to absorb the cell sap again, the injection process is complicated, the method can inject the culture fluid volume required by a plurality of culture dishes 8 into the injector at one time, then the injected cell sap is vertically and visually observed, after the cell sap is injected into the target volume, the injector is moved to the culture dish 8 at the next position for injecting the cell sap, the injection of the cell sap into the plurality of culture dishes 8 at one time is convenient, the injection volume is accurate and convenient to run through, the culture box 4 is positively provided with the culture tank 5 for placing the culture box 6 and the sealing cover 7, the top surface of the sealing cover 7 is covered by the sliding of the limiting strip plate 11, the cell sap in the same column can be taken out or placed one by one without influencing the environment in other culture dishes 8, keep not influenced when taking out and placing the culture solution between the adjacent culture dish 8 to reduce the environmental error between the adjacent culture dish 8, guarantee the precision that cell culture detected.

Spacing tongue 12 runs through with the same row of water conservancy diversion hole 9 of sealed 7 top surfaces, spacing slat 11 bottom surface both sides and spacing tongue 12 block, spacing tongue 12 cross-section is the type of falling T, spacing slat 11 regular cross-section is the I shape, keep spacing slat 11 and the stable block of spacing tongue 12, prevent to drop, incubator 4 outside edge all adopts fillet smooth processing, instrument display screen 1 outside edge all adopts fillet smooth processing, cultivate 6 top surface four corners and the block of sealed 7 bottom surfaces four corners of lid, incubator 5 inside all is equipped with and cultivates box 6 and sealed lid 7, can detect simultaneously the cell sap inside a plurality of cultivation boxes 6, incubator 6 and sealed 7 outside edge all adopt fillet processing, spacing tongue 12 one end runs through with sealed lid 7 top surface one side, spacing slat 11 is the same with sealed lid 7 top surface width, spacing slat 11 width is 3/2 of water conservancy diversion hole 9 diameter, keep spacing slat 11 and water conservancy diversion hole 9's sealed environment, reduce and detect data error.

In the present application, unless expressly stated or limited otherwise, the recitation of a first feature "on" or "under" a second feature may include the recitation of the first and second features being in direct contact, and may also include the recitation of the first and second features not being in direct contact, but being in contact with another feature between them. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The foregoing shows and describes the basic principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a class organ is cultivateed and is detected ELIASA, includes instrument display screen (1) and incubator (4), its characterized in that: incubator (4) top surface one side is fixed and is equipped with instrument display screen (1), incubator (4) openly vertical direction has evenly seted up culture tank (5), culture tank (5) inside is equipped with cultivates box (6), it is equipped with sealed lid (7) to cultivate box (6) top surface block, it evenly is equipped with culture dish (8) to cultivate box (6) surface, sealed lid (7) top surface corresponds position department with culture dish (8) and has seted up water conservancy diversion hole (9), sealed lid (7) top surface is equipped with spacing groove (10) along water conservancy diversion hole (9) edge, and spacing groove (10) are blind groove, sealed lid (7) top surface linear array has seted up spacing straight flute (12), spacing straight flute (12) inside block is equipped with spacing slat (11).

2. The microplate reader for organoid culture detection of claim 1, wherein: spacing straight flute (12) run through with same water conservancy diversion hole (9) that are listed as of sealed lid (7) top surface, spacing slat (11) bottom surface both sides and spacing straight flute (12) block, spacing straight flute (12) cross-section is the type of falling T, spacing slat (11) normal cross-section is the I shape.

3. The microplate reader for organoid culture detection of claim 1, wherein: cultivate box (6) top surface four corners and sealed lid (7) bottom surface four corners block, cultivate that the inside culture box (6) and sealed lid (7) of all being equipped with of groove (5), cultivate box (6) and sealed lid (7) outside edge and all adopt the fillet to handle.

4. The microplate reader for organoid culture detection of claim 1, wherein: one side bolt joint that instrument display screen (1) was kept away from to incubator (4) top surface is equipped with storage tank (3), incubator (4) top surface is equipped with regulation and control button (2), regulation and control button (2) and instrument display screen (1) electric connection.

5. The microplate reader for organoid culture detection of claim 1, wherein: one end of the limiting straight groove (12) penetrates through one side of the top surface of the sealing cover (7), the width of the limiting strip plate (11) is the same as that of the top surface of the sealing cover (7), and the width of the limiting strip plate (11) is 3/2 of the diameter of the flow guide hole (9).

6. The microplate reader for organoid culture detection of claim 1, wherein: the outer edge of the incubator (4) is subjected to round angle smooth processing, and the outer edge of the instrument display screen (1) is subjected to round angle smooth processing.