CN105619510A

CN105619510A - Agar punching device

Info

Publication number: CN105619510A
Application number: CN201610077295.1A
Authority: CN
Inventors: 田晔; 代鹏; 宋峰亮; 孔彦龙; 许鹏程
Original assignee: Anhui University of Science and Technology
Current assignee: Anhui University of Science and Technology
Priority date: 2016-02-01
Filing date: 2016-02-01
Publication date: 2016-06-01
Anticipated expiration: 2036-02-01
Also published as: CN105619510B

Abstract

The invention discloses an agar punching device and relates to the technical field of experimental facilities. The agar punching device comprises a base, a support, a limit valve, a rack, a movable table, a gear, a front baffle, a rear baffle, a side baffle, an air bag, gauze, an agar storage box, a punching head, a clamping valve, a cutter, a key and a lifting switch. The punching head can be positioned and made to rise and fall through a gear-rack fine adjustment structure and the limiting valve, after agar is cut through the cutter on the punching head, the cut agar is sucked out through the air bag and stored in the box, and thus a non-through hole with the planned depth is punched. Due to the fact that the non-through hole with a bottom can be punched, the situation that experimental errors are caused due to the fact that a liquid sample added into the hole leaks toward the periphery from the bottom can be avoided, and a plurality of holes which are spaced from one another by a fixed distance can be punched according to requirements by quantitatively rotating the base provided with scales. The punching device is easy and convenient to operate and low in price and has high promotional value.

Description

A kind of Gelose punch

Technical field

The present invention relates to technical field of experiment equipment, be specifically related to a kind of Gelose punch.

Background technology

Biology and the many experiments of medical science need punch on the agar being layered on microscope slide or culture dish surface, such as immunodiffusion test, Antibiotics resistance test etc. The quality of card punch punching is very important on the impact of experimental result, so far still without desirable, special unified card punch.

Mostly existing card punch is to be inserted directly into agar to bottom microscope slide or culture dish, then taking out agar in hole, get the through hole being through to bottom agar with stainless-steel thin-wall pipe or glass tubing. But sample is when adding in hand-hole, it is possible to from bottom leakage, experimental result can be affected. Some investigators will pass through and adds the non-through hole melting the formation post forming of agar back cover in hole, operation inconvenience, and it sometimes appear that bottom injustice also can affect experimental result. It is particularly important accordingly, it is capable to non-through hole is got in no one-shot forming.

In an experiment, such as drug sensitivity assay, every piece of culture medium usually needs to get multiple hole by regulation pitch-row and meets the requirement of experiment. The card punch of existing single perforating head is bad assurance pitch-row when punching, it is necessary to measures bottom agar and punches successively after marking; Though the card punch of some Flos Mume pass can directly get uniform bore from hole. But when multiple holes head punches, it is easy to make agar be split up, agar edge may be dug simultaneously. Therefore, during punching can accurate assurance pitch-row and don't damage agar be also problem demanding prompt solution.

Summary of the invention

For the deficiencies in the prior art, disclosure one Gelose punch, it is possible to solve one-shot forming cracking down illegal activities through hole and hitting the target determine from the problem of porous.

For realizing object above, the present invention is achieved by the following technical programs:

A kind of Gelose punch, including base, support, limit valve, tooth bar, mobile platform, gear, front apron, backboard, side shield, air bag, gauze, agar storage box, perforating head, screens valve, cutting knife, key and direction switch, support is arranged on base, and vertical with base; Tooth bar is arranged on support by screw; Mobile platform is nested in support with on tooth bar, and tooth bar closes with mobile decometer; Connecting agar storage box and perforating head on the right side of mobile platform, connect gear by front apron backboard, direction switch is connected with gear by axle.

Preferably, described limit valve is arranged on support, and limit valve is provided with bolt.

Preferably, described perforating head upper end is provided with agar storage box, and agar storage box is threadedly attached on mobile platform; Perforating head is double; two stainless steel tube structures, and interior pipe connects agar storage box, and on inner pipe, the outer tube mouth of pipe is provided with cutting knife to outer tube sleeve; Being provided with conduit in the middle part of outer tube, be provided with the screens valve of projection in the middle part of interior pipe, screens valve can move in conduit.

Preferably, described cutting knife adopts finer wire material.

Preferably, described agar storage box upper end has aperture and is connected with the air bag with check valve, and air bag forms closed structure with agar storage box and perforating head; It is provided with gauze in agar storage box.

Preferably, described base has scale.

Disclosure one Gelose punch, it is possible to get non-through hole, has stopped the phenomenon of through hole bottom liquid sample leakage, has improve the accuracy of experimental result; With reference to the scale on base during punching, it is accurately controlled pitch-row and beats multiple hole successively, eliminate the trouble of punching pre-test labelling, further increase the accuracy of experimental result, time saving and energy saving; Card punch arranges agar storage box, and the agar got utilizes the time being both needed to the agar that cleaning is excised after eliminating punching every time in negative pressure suction punching box.

Accompanying drawing explanation

In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.

Fig. 1 is the main TV structure schematic diagram of the present invention;

Fig. 2 is the plan structure schematic diagram of the present invention;

Fig. 3 is the structural representation of limit valve in the present invention;

Fig. 4 is the structural representation of perforating head in the present invention;

Fig. 5 is the structural representation of base scale in the present invention; .

In figure 1, base, 2, support, 3, limit valve, 4, tooth bar, 5, mobile platform, 6, gear, 7, front apron, 8, air bag, 9, gauze, 10, agar storage box, 11, side shield, 12, perforating head, 13, screens valve, 14, cutting knife, 15, backboard, 16, key, 17, direction switch.

Detailed description of the invention

For making the purpose of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is a part of embodiment of the present invention, rather than whole embodiments. Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.

Referring to Fig. 1 and Fig. 2, a kind of Gelose punch, including: the composition such as base 1, support 2, limit valve 3, tooth bar 4, mobile platform 5, gear 6, front apron 7, backboard 15, side shield 11, air bag 8, gauze 9, agar storage box 10, perforating head 12, screens valve 13, cutting knife 14, key 16, direction switch 17. Support 2 is welded on base 1, and vertical with base 1. Tooth bar 4 invests support 2 by screw. Mobile platform 5 is nested in support 2 with on tooth bar 4, and tooth bar 4 is designed to special shape and engages with mobile platform 5 so that mobile platform 5 can complete lifting process on support 2. Connecting agar storage box 10 and perforating head 12 on the right side of mobile platform 5, connect gear 6 by front apron 7 backboard 15, direction switch 16 is connected with gear 6 by axle, rotates direction switch 17 and can control the lifting of perforating head 12.

Referring to Fig. 1, Fig. 2 and Fig. 3, limit valve 3 is nested on support 2 by mode as depicted, and limit valve 3 is provided with bolt, can be used to the position of fixing limit valve 3. Stop declining when mobile platform 5 drops to the position of limit valve 3, thus controlling the extreme lower position that perforating head 12 declines. Once can pass through limit valve 3 in experiment makes the degree of depth of punched hole identical, simple and quick.

Referring to Fig. 1, Fig. 4, perforating head 12 upper end is provided with agar storage box 10, and agar storage box 10 is threadedly attached on mobile platform 5. Perforating head 12 is double; two stainless steel tube structures, and interior pipe connects agar storage box 10, and on inner pipe, the outer tube mouth of pipe is provided with cutting knife 14 to outer tube sleeve. And inner and outer pipes diameter is close, the gap between inner and outer pipes is negligible. Being provided with conduit in the middle part of outer tube, be provided with screens valve in the middle part of interior pipe, screens valve can move in conduit, thus outer tube can being made to rotate but not coming off. When perforating head 12 enters agar to after Suitable depth, turn outer tube, agar in pipe can be cut off by cutting knife 14, to reach the purpose of cutting. Owing to monolayer steel pipe is inserted into agar, when pipe in agar thickness account for gross thickness ratio more time, rotating steel tube can make agar in pipe drive residue fraction to be introduced into managing the rotation of interior agar, thus destroying the structure of the agar being introduced in pipe, therefore the present invention adopts interior pipe motionless, the structure of spun outer tube.

Agar storage box 10 upper end has aperture and is connected with the air bag 8 with check valve, and air bag 8 forms closed structure with agar storage box 10 and perforating head 12. After the agar entered in pipe is cut-off, extruding gasbag 8, cut-off agar can be drawn in agar storage box 10. It is provided with gauze 9 in agar storage box 10, can prevent the agar after being cut from entering in air bag 8.

Referring to Fig. 5, base 1 is marked with scale, and the intersection point of two row scales is the position of punching. For pitch-row for 3cm, first pass through scale and find agar plates center, make a call to first hole. Then move right 3cm by culture dish, makes a call to second hole. According to wanting punched hole number, centered by first hole, second hole is rotated clockwise to the position of corresponding hole count. As made a call to 6 holes, then turn clockwise 360 ��/6=60 ��, namely corresponding " 6 " position. In like manner, make a call to 7 holes, then turn clockwise 360 ��/7 �� 51.43 ��, namely corresponding " 7 position ". Make a call to the 3rd hole, then still centered by first hole, the 3rd hole is rotated clockwise to relevant position. By that analogy, all of hole is got.

Disclosure one Gelose punch in sum, by the fine tuning structure of rack-and-pinion and limit valve, can positioned-lifting perforating head, on perforating head cutting knife cutting agar after, and it is stored in the agar sucking-off cut out in box with air bag, thus getting the non-through hole of planned depth. Owing to non-through hole with the end can be got, the liquor sample adding in hand-hole can be avoided to spill towards periphery from bottom and the experimental error that causes, separately can pass through quantitatively to rotate set graduated base get on request fixing hole from multiple holes. This card punch is easy and simple to handle, cheap, has stronger promotional value.

It should be noted that, in this article, the relational terms of such as first and second or the like is used merely to separate an entity or operation with another entity or operating space, and not necessarily requires or imply the relation that there is any this reality between these entities or operation or sequentially. And, term " includes ", " comprising " or its any other variant are intended to comprising of nonexcludability, so that include the process of a series of key element, method, article or equipment not only include those key elements, but also include other key elements being not expressly set out, or also include the key element intrinsic for this process, method, article or equipment. When there is no more restriction, statement " including ... " key element limited, it is not excluded that there is also other identical element in including the process of described key element, method, article or equipment.

Above example only in order to technical scheme to be described, is not intended to limit; Although the present invention being described in detail with reference to previous embodiment, it will be understood by those within the art that: the technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature is carried out equivalent replacement; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims

1. a Gelose punch, including base, support, limit valve, tooth bar, mobile platform, gear, front apron, backboard, side shield, air bag, gauze, agar storage box, perforating head, screens valve, cutting knife, key and direction switch, it is characterized in that: support is arranged on base, and vertical with base; Tooth bar is arranged on support by screw; Mobile platform is nested in support with on tooth bar, and tooth bar closes with mobile decometer; Connecting agar storage box and perforating head on the right side of mobile platform, connect gear by front apron backboard, direction switch is connected with gear by axle.

2. Gelose punch as claimed in claim 1, it is characterised in that: described limit valve is arranged on support, and limit valve is provided with bolt.

3. Gelose punch as claimed in claim 1, it is characterised in that: described perforating head upper end is provided with agar storage box, and agar storage box is threadedly attached on mobile platform; Perforating head is double; two stainless steel tube structures, and interior pipe connects agar storage box, and on inner pipe, the outer tube mouth of pipe is provided with cutting knife to outer tube sleeve; Being provided with conduit in the middle part of outer tube, be provided with the screens valve of projection in the middle part of interior pipe, screens valve can move in conduit.

4. Gelose punch as claimed in claim 3, it is characterised in that: described cutting knife adopts finer wire material.

5. Gelose punch as claimed in claim 1, it is characterised in that: described agar storage box upper end has aperture and is connected with the air bag with check valve, and air bag forms closed structure with agar storage box and perforating head; It is provided with gauze in agar storage box.

6. Gelose punch as claimed in claim 1, it is characterised in that: described base has scale.