CN105619510B - A kind of Gelose punch - Google Patents

A kind of Gelose punch Download PDF

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Publication number
CN105619510B
CN105619510B CN201610077295.1A CN201610077295A CN105619510B CN 105619510 B CN105619510 B CN 105619510B CN 201610077295 A CN201610077295 A CN 201610077295A CN 105619510 B CN105619510 B CN 105619510B
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China
Prior art keywords
agar
storage box
perforating head
valve
mobile station
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CN201610077295.1A
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Chinese (zh)
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CN105619510A (en
Inventor
田晔
代鹏
宋峰亮
孔彦龙
许鹏程
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Anhui University of Science and Technology
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Anhui University of Science and Technology
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Priority to CN201610077295.1A priority Critical patent/CN105619510B/en
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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B26HAND CUTTING TOOLS; CUTTING; SEVERING
    • B26FPERFORATING; PUNCHING; CUTTING-OUT; STAMPING-OUT; SEVERING BY MEANS OTHER THAN CUTTING
    • B26F1/00Perforating; Punching; Cutting-out; Stamping-out; Apparatus therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B26HAND CUTTING TOOLS; CUTTING; SEVERING
    • B26DCUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
    • B26D7/00Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B26HAND CUTTING TOOLS; CUTTING; SEVERING
    • B26DCUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
    • B26D7/00Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
    • B26D7/18Means for removing cut-out material or waste

Abstract

The present invention discloses a kind of Gelose punch, is related to technical field of experiment equipment.Including base, support, limit valve, tooth bar, mobile station, gear, front apron, backboard, side shield, air bag, gauze, agar the storage box, perforating head, screens valve, cutting knife, key and direction switch.By the fine tuning structure and limit valve of rack-and-pinion, can positioned-lifting perforating head, on perforating head cutting knife cutting agar after, the agar that will be cut out with air bag is suctioned out and is stored in box, so as to get the non-through hole of planned depth.Due to non-through hole with the end can be got, experimental error caused by can avoiding adding the liquor sample in hole from being spilt to surrounding from bottom, separately can by quantitative rotation set graduated base get on request fixing hole away from multiple holes.The card punch is easy to operate, cheap, with stronger promotional value.

Description

A kind of Gelose punch
Technical field
The present invention relates to technical field of experiment equipment, and in particular to a kind of Gelose punch.
Background technology
Need be layered on punching on the agar on slide or culture dish surface in biology and many experiments of medical science, it is such as immune Diffusion experiment, Antibiotics resistance test etc..The influence of the quality to experimental result of card punch punching is very important, so far still without Preferably, special unified card punch.
Existing card punch is mostly to be inserted directly into agar to slide or culture dish bottom with stainless-steel thin-wall pipe or glass tube, Agar in hole is then taken out, the through hole for being through to agar bottom is got.But sample may be let out when adding in hole from bottom Leakage, influences experimental result.Some investigators add the non-through hole for melting the formation post forming of agar back cover in will pass through hole, Operation inconvenience, and it sometimes appear that bottom injustice can also influence experimental result.Accordingly, it is capable to non-through hole is got in no one-shot forming show Obtain particularly important.
In an experiment, such as drug sensitivity assay, every piece of culture medium usually needs to get multiple holes by regulation pitch-row to meet The requirement of experiment.The card punch of existing single perforating head punching when it is bad assurance pitch-row, it is necessary to be done in the measurement of agar bottom Punched successively after mark;Though the card punch of some plum blossom passes can directly get uniform bore away from hole.But multiple hole heads are simultaneously During punching, easily it is split up agar, agar edge may be dug.Therefore, pitch-row and not can be accurately held during punching It is also problem demanding prompt solution to damage agar.
The content of the invention
In view of the shortcomings of the prior art, the present invention discloses a kind of Gelose punch, can solve the problem that one-shot forming cracking down illegal activities runs through Hole and hit the target determine away from porous problem.
To realize object above, the present invention is achieved by the following technical programs:
A kind of Gelose punch, including base, support, limit valve, tooth bar, mobile station, gear, front apron, backboard, side Baffle plate, air bag, gauze, agar the storage box, perforating head, screens valve, cutting knife, key and direction switch, support are arranged on base, and It is vertical with base;Tooth bar is arranged on support by screw;Mobile station is nested on support and tooth bar, tooth bar and mobile decometer Close;Mobile station right side connection agar the storage box and perforating head, by front apron backboard connect gear, direction switch by axle with Gear is connected.
Preferably, the limit valve is arranged on support, and limit valve is provided with bolt.
Preferably, the perforating head upper end is provided with agar the storage box, and agar the storage box is threadedly attached in mobile station; Perforating head is double stainless steel tube structures, and inner tube connects agar the storage box, and on inner pipe, the outer tube mouth of pipe is provided with cutting knife to outer tube sleeve;Outward Pipe middle part is provided with conduit, and inner tube middle part is provided with the screens valve of projection, and screens valve can be moved in conduit.
Preferably, the cutting knife uses finer wire material.
Preferably, the agar the storage box upper end is provided with aperture and is connected with the air bag with check valve, air bag and agar The storage box and perforating head form closed structure;Gauze is provided with agar the storage box.
Preferably, there is scale on the base.
The present invention discloses a kind of Gelose punch, can get non-through hole, has prevented through hole bottom liquid sample and has let out The phenomenon of leakage, improves the accuracy of experimental result;With reference to the scale on base during punching, accurately control pitch-row and beat many successively Individual hole, eliminates the trouble of measurement markers before punching, further increases the accuracy of experimental result, time saving and energy saving;Card punch Set agar the storage box, the agar that will be got using negative pressure suction punching box in eliminate every time punching after be both needed to cleaning excision The time of agar.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is main structure diagram of the invention;
Fig. 2 is overlooking the structure diagram of the invention;
Fig. 3 is the structural representation of limit valve in the present invention;
Fig. 4 is the structural representation of perforating head in the present invention;
Fig. 5 is the structural representation of base scale in the present invention;.
1, base in figure, 2, support, 3, limit valve, 4, tooth bar, 5, mobile station, 6, gear, 7, front apron, 8, air bag, 9, Gauze, 10, agar the storage box, 11, side shield, 12, perforating head, 13, screens valve, 14, cutting knife, 15, backboard, 16, key, 17, Direction switch.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is A part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
Referring to Fig. 1 and Fig. 2, a kind of Gelose punch, including:Base 1, support 2, limit valve 3, tooth bar 4, mobile station 5, tooth Wheel 6, front apron 7, backboard 15, side shield 11, air bag 8, gauze 9, agar the storage box 10, perforating head 12, screens valve 13, cutting knife 14th, the composition such as key 16, direction switch 17.Support 2 is welded on base 1, and vertical with base 1.Tooth bar 4 invests branch by screw Frame 2.Mobile station 5 is nested on support 2 and tooth bar 4, and tooth bar 4 is designed to special shape and engages with mobile station 5 so that mobile station 5 can complete lifting process on support 2.The right side of mobile station 5 connects agar the storage box 10 and perforating head 12, by front apron 7 Backboard 15 connects gear 6, and direction switch 16 is connected by axle with gear 6, rotates the i.e. controllable perforating head 12 of direction switch 17 Lifting.
Referring to Fig. 1, Fig. 2 and Fig. 3, limit valve 3 is nested on support 2 by mode as depicted, and limit valve 3 is provided with Bolt, can be used to fix the position of limit valve 3.Stop declining when mobile station 5 drops to the position of limit valve 3, so as to control The extreme lower position that perforating head processed 12 declines.By limit valve 3 depth of punched hole can be made identical in once testing, it is simple to operate Fast.
Referring to Fig. 1, Fig. 4, the upper end of perforating head 12 is provided with agar the storage box 10, and agar the storage box 10 is threadedly attached in shifting On dynamic platform 5.Perforating head 12 is double stainless steel tube structures, inner tube connection agar the storage box 10, outer tube sleeve on inner pipe, the outer tube mouth of pipe It is provided with cutting knife 14.And inside and outside pipe diameter is close, the gap between inner and outer pipes is negligible.Outer tube middle part is provided with conduit, inner tube Middle part is provided with screens valve, and screens valve can be moved in conduit, so as to outer tube can be made to rotate but will not fall off.When perforating head 12 enters After agar to Suitable depth, turn outer tube, cutting knife 14 can be by agar cut-out in pipe, to reach the purpose of cutting.Due to individual layer Steel pipe is inserted into agar, when in pipe agar thickness account for gross thickness ratio it is more when, it is surplus that rotating steel tube can drive in pipe agar Remaining fraction is introduced into managing the rotation of interior agar, and so as to destroy the structure of the agar being introduced into pipe, therefore the present invention is used Inner tube is motionless, the structure of spun outer tube.
The upper end of agar the storage box 10 is provided with aperture and is connected with the air bag 8 with check valve, air bag 8 and agar the storage box 10 And perforating head 12 forms closed structure.After being cut off into the agar in pipe, extruding gasbag 8, you can the fine jade that will be cut off Fat is drawn into agar the storage box 10.Gauze 9 is provided with agar the storage box 10, can prevent the agar after being cut from entering air bag 8 It is interior.
Referring to Fig. 5, base 1 is marked with scale, and the intersection point of two row scales is the position of punching.It is first so that pitch-row is as 3cm as an example First pass through scale and find agar plates center, make a call to first hole.Then culture dish is moved right 3cm, makes a call to second Hole.According to desired punched hole number, centered on first hole, second hole is rotated clockwise to the position of correspondence hole count. Such as make a call to 6 holes, then turn clockwise 360 °/6=60 °, i.e., corresponding " 6 " position.Similarly, 7 holes are made a call to, is then turned clockwise 360 °/7 51.43 ° of ≈, i.e., corresponding " 7 position ".The 3rd hole is made a call to, then still centered on first hole, by the 3rd Kong Shun Hour hands are rotated to relevant position.By that analogy, all of hole is got.
The present invention discloses a kind of Gelose punch in sum, by the fine tuning structure and limit valve of rack-and-pinion, can determine Position lifting perforating head, after the cutting knife cutting agar on perforating head, the agar that will be cut out with air bag is suctioned out and is stored in box, so that Get the non-through hole of planned depth.Due to non-through hole with the end can be got, can avoid adding the liquor sample in hole the bottom of from Portion spilt to surrounding caused by experimental error, separately can by quantitative rotation set graduated base get on request fixing hole away from Multiple holes.The card punch is easy to operate, cheap, with stronger promotional value.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposited between operating In any this actual relation or order.And, term " including ", "comprising" or its any other variant be intended to Nonexcludability is included, so that process, method, article or equipment including a series of key elements not only will including those Element, but also other key elements including being not expressly set out, or also include being this process, method, article or equipment Intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that Also there is other identical element in process, method, article or equipment including the key element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although with reference to the foregoing embodiments The present invention has been described in detail, it will be understood by those within the art that:It still can be to foregoing each implementation Technical scheme described in example is modified, or carries out equivalent to which part technical characteristic;And these modification or Replace, do not make the spirit and scope of the essence disengaging various embodiments of the present invention technical scheme of appropriate technical solution.

Claims (3)

1. a kind of Gelose punch, including base, support, limit valve, tooth bar, mobile station, gear, front apron, backboard, side block Plate, air bag, gauze, agar the storage box, perforating head, screens valve, cutting knife, key and direction switch, it is characterised in that:Support is arranged on It is on base and vertical with base;Tooth bar is arranged on support by screw;Mobile station is nested on support and tooth bar, tooth bar with Mobile station engages;The connection of mobile station right side agar the storage box and perforating head, gear, direction switch are connected by front apron backboard It is connected with gear by axle;The perforating head upper end is provided with agar the storage box, and agar the storage box is threadedly attached in mobile station On;Perforating head is double stainless steel tube structures, and inner tube connects agar the storage box, and on inner pipe, the outer tube mouth of pipe is provided with cutting knife to outer tube sleeve; Outer tube middle part is provided with conduit, and inner tube middle part is provided with the screens valve of projection, and screens valve can be moved in conduit;The agar storage Box upper end is provided with aperture and is connected with the air bag with check valve, and air bag forms closed knot with agar the storage box and perforating head Structure;Gauze is provided with agar the storage box;There is scale on the base.
2. Gelose punch as claimed in claim 1, it is characterised in that:The limit valve is arranged on support, is set on limit valve There is bolt.
3. Gelose punch as claimed in claim 1, it is characterised in that:The cutting knife uses finer wire material.
CN201610077295.1A 2016-02-01 2016-02-01 A kind of Gelose punch Active CN105619510B (en)

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106271692B (en) * 2016-08-29 2018-03-13 浙江柏同机器人科技股份有限公司 For the device continuously punched to workpiece
CN106995777A (en) * 2017-06-09 2017-08-01 钟金凤 A kind of culture medium punches workbench
CN108152110A (en) * 2017-12-20 2018-06-12 广州市华南农大生物药品有限公司 A kind of agar hole making device
CN109175434A (en) * 2018-11-16 2019-01-11 贵州大学 Drilling machine is used in a kind of machining
CN109434939B (en) * 2018-12-19 2023-09-08 江苏煜宇塑料机械科技有限公司 Plastic tubing puncher
CN113977684B (en) * 2021-11-01 2023-04-14 柳州市人民医院 Agar hole puncher for medical immunity experiments

Citations (6)

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Publication number Priority date Publication date Assignee Title
US4471677A (en) * 1982-02-09 1984-09-18 Rhone-Poulenc S.A. Device for producing cylindrical cavities in a layer of gelled material
CN201265156Y (en) * 2008-09-26 2009-07-01 大庆油田有限责任公司 Frozen earth sample preparation machine
CN102175478A (en) * 2011-01-28 2011-09-07 河海大学 System and method for sampling soil
CN203141581U (en) * 2013-03-29 2013-08-21 中国农业科学院植物保护研究所 Gel punching device
CN103331790A (en) * 2013-07-16 2013-10-02 扬州市胜达电动工具有限公司 Multifunctional tenoning machine
CN205325839U (en) * 2016-02-01 2016-06-22 安徽理工大学 Agar hole puncher

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4471677A (en) * 1982-02-09 1984-09-18 Rhone-Poulenc S.A. Device for producing cylindrical cavities in a layer of gelled material
CN201265156Y (en) * 2008-09-26 2009-07-01 大庆油田有限责任公司 Frozen earth sample preparation machine
CN102175478A (en) * 2011-01-28 2011-09-07 河海大学 System and method for sampling soil
CN203141581U (en) * 2013-03-29 2013-08-21 中国农业科学院植物保护研究所 Gel punching device
CN103331790A (en) * 2013-07-16 2013-10-02 扬州市胜达电动工具有限公司 Multifunctional tenoning machine
CN205325839U (en) * 2016-02-01 2016-06-22 安徽理工大学 Agar hole puncher

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