CN107617057A - Application of the Dendrobidium huoshanness extract in enhancing immunology - Google Patents
Application of the Dendrobidium huoshanness extract in enhancing immunology Download PDFInfo
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Abstract
It is used to alleviate diabetic condition in the application of enhancing immunology, specially application of the Dendrobidium huoshanness extract in enhancing immune drug is prepared, the medicine the invention discloses a kind of Dendrobidium huoshanness extract；The acquisition of Dendrobidium huoshanness extract：(1) Dendrobidium huoshanness is cut into slices, grind into powder after drying；Ethanol decolorization element, water extraction 5h；Heavy alcohol overnight；It is evaporated；(2) Sevage joint Papain enzyme process is taken to take off albumen, dialysis, vacuum freeze drying；(3) anion exchange chromatography isolates and purifies, and collects eluent, concentrates, filtration.Advantages of the present invention is：Macroglobulin, macrophage activating factor (MAF) and quick stress reaction is immunized to improve the immune response ability of body by raising α 2 in the Dendrobidium huoshanness extract of the present invention, increase the enzyme content of glutathione by raising glutathione synthetase, the oxidative damage brought by fat and high fat of blood is reduced, so as to alleviate diabetic condition.
Description
Technical field
The invention belongs to pharmaceutical field, is related to a kind of new application of Chinese medical extract, and in particular to a kind of Dendrobidium huoshanness carries
Take application of the thing in enhancing immunology.
Background technology
Dendrobidium huoshanness (scientific name：Dendrobium huoshanense), there is the open country of the title of " medicine circle giant panda " in the world
Raw Dendrobidium huoshanness, also known as rice dry measure used in former times, away from modern existing more than 200 years history.Dendrobidium huoshanness originates in the ground such as China Huoshan, is state
The first protection kind, is the superfine product in medicinal dendrobium in more than 200 Rare Kinds in imminent danger of family's modern Chinese herbal medicine key special subjects, and suddenly
Mountain county is exclusive, possesses the rare traditional Chinese medicine of original producton location geographical sign, is described as " first of Chinese nine big celestial grass " in history, " is good for
Health soft gold ",《Legendary god of farming's book on Chinese herbal medicine》、《Compendium of Materia Medica》It is on the books.Polysaccharide contained by Dendrobidium huoshanness can increase substantially human body
Interior SOD (main matter of anti-aging), in strengthen immunity, antifatigue, anti-aging and promote cancer cell-apoptosis etc.
There is obvious effect, the effect of so as to reach health-care.In addition to Dendrobidium huoshanness, other medicinal dendrobiums (huoshan dendrobium candidum and
Huoshan Dendrobium Moniliforme etc.) also there are similar health care and medical value.
At present, the pathogenesis about diabetes is mainly considered to be drawn by polygenes induction and environmental interaction at present
Caused by the organism metabolism dysfunction risen, wherein using hyperglycaemia as its outstanding feature the most.
Common diabetes mainly include I type and II type.
IDDM itself can not caused by being badly damaged or destroy mainly due to race's island B cell that autoimmunity mediates
Excreting insulin, insulin is caused definitely to lack, it is necessary to long-term to maintain the steady of internal blood glucose value by injection of exogenous insulin
It is fixed, so T-shaped diabetes are also referred to as the diabetes of insulin-dependent, because IDDM was usually fallen ill before 35 years old, again
Referred to as adolescent diabetes.IDDM onset is anxious, and the random symptom of metabolism element is obvious, and patient Tong Chang has inclining for Ugly disease acid poisonings
To；The bad patient of final glycemic control, easily it may suffer from uremia with nephrosis, severe patient.
Type II diabetes is mainly with hyperglycemia caused by insulin resistance or insulin secretion relative deficiency, and patient is not
Insulin injection is needed to treat, therefore the also known as diabetes of non-insulin-depending type.Type II diabetes is more common in adult, suffers from
Person's majority is with obesity symptom, its slow, patient's early stage non-evident sympton of falling ill, generally without DKA tendency.II type sugar
Urine disease occupies the ratio of diabetic about 95%, turns into " main force " of diabetic.Can with the growth state of an illness at age
It can aggravate, and with multiple complications, have a strong impact on patient body health.
Diabetes are Metabolic syndrome symptoms caused by polygenes, so can not divide from single direction hypoglycemic mechanism
Analysis.At present, it is main including adjusting blood glucose by improving the damage of islet cells to probing into for polysaccharide hypoglycemic mechanism, pass through promotion
Insulin-sensitizing effect carrys out regulating and controlling blood sugar, by activating PPAR σ and PPAR γ come hypoglycemic and reducing blood lipid, influences alphalise starch enzyme activity
Property, increase sugar and store and cooperate with raising anti-oxidant to prevent hyperglycaemia.
RNA-seq sequencing technologies come hunting efficiency target gene and associated metabolic signal path from genomics profound level, are
Study of disease provides the accurately assignment of genes gene mapping, and the sample of different experimental conditions processing can reflect Differential Gene in transcript profile
The critical sites of (up-regulated gene and down-regulated gene) and signal path change, and this provides more accurate genome to researcher
Learn scientific data.
Inventor, with reference to RNA-seq sequencing technologies, is had found, suddenly by constantly making great efforts and studying by the experiment of science
The mountain stem of noble dendrobium and its extract are strengthening the new mechanism of action of immunology.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of Huoshan stone based on the new mechanism of action
Application of the dry measure used in former times extract in enhancing immunology.
The present invention is achieved by the following technical solutions：Dendrobidium huoshanness extract is in the application of enhancing immunology, tool
Body is that Dendrobidium huoshanness extract is preparing the application in strengthening immune drug, and the medicine is for alleviating diabetic condition；Wherein, institute
The acquisition methods for stating Dendrobidium huoshanness extract are：
(1) Dendrobidium huoshanness is taken to cut into slices, grind into powder after 50 DEG C of drying；80% ethanol sloughs pigment, 50 DEG C of water extraction 5h；
95% ethanol, overnight sink alcohol；60% is evaporated, and obtains Dendrobidium huoshanness Thick many candies；
(2) take Sevage joint Papain enzyme process to take off albumen, then dialysed, vacuum freeze drying, obtain Huoshan
Dendrobium polysaccharide；
(3) selection anion exchange chromatography isolates and purifies to Dendrobidium huoshanness polysaccharide, collects target eluent, concentration
To 100-200mg crude drugs/mL, filter and produce.
One of preferred embodiment as the present invention, in the obtaining step (2) of the Dendrobidium huoshanness extract, takes Sevage
The Deproteinated specific method of joint Papain enzyme process is：2% papain is added in the Dendrobidium huoshanness Thick many candies of acquisition,
50 DEG C of water-bath 2h, according still further to Dendrobidium huoshanness Thick many candies solution：N-butanol：Chloroform volume ratio example is 25：1：5 add reaction reagent,
Operate 4-5 times repeatedly, until without albumin layer.
One of preferred embodiment as the present invention, the application are acted as by strengthening immune response and improving oxidative damage
With.
As one of preferred embodiment of the present invention, macroglobulin, huge is immunized by raising α -2 in the Dendrobidium huoshanness extract
Phagocyte activation factor and quick stress reaction improve the immune response ability of body.
One of preferred embodiment as the present invention, the Dendrobidium huoshanness extract is increased by raising glutathione synthetase
The enzyme content of glutathione, reduce the oxidative damage brought by fat and high fat of blood.
One of preferred embodiment as the present invention, the medicine is the medicine using Dendrobidium huoshanness extract as main active
Compositions.
One of preferred embodiment as the present invention, described pharmaceutical composition drug containing active substances 10-100mg, remaining is
Pharmaceutically acceptable carrier.
One of preferred embodiment as the present invention, the pharmaceutically acceptable carrier specifically includes：Mannitol, sorb
Alcohol, sorbic acid, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, dimension life
Plain A, vitamin C, vitamin E, vitamin D, azone, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the carbonate of monovalence alkali metal, acetate,
Phosphate, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose,
Fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose, alginates, gelatin, poly- second
Alkene pyrrolidone, glycerine, propane diols, ethanol, soil temperature 60-80, Span-80, beeswax, lanolin, atoleine, hexadecanol, fine jade
Fat, triethanolamine, basic amino acid, urea, allantoin, calcium carbonate, calcium bicarbonate, surfactant, polyethylene glycol, ring paste
Essence, beta-schardinger dextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate, vegetable oil, phosphatide, polyethylene glycol phosphorus
Fat, oleic acid, EDTA.
One of preferred embodiment as the present invention, the medicine is any pharmaceutical dosage form.
One of preferred embodiment as the present invention, the pharmaceutical dosage form specifically includes：Tablet, sugar coated tablet, Film coated tablets
Agent, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, mouth containing agent, granule, electuary, pill, powder, cream
Agent, sublimed preparation, supensoid agent, solution, injection, suppository, ointment, emplastrum, creme, spray, drops, patch.
The present invention compared with prior art the advantages of be：The present invention makees for prior art to Dendrobidium huoshanness extract pharmacology
With the deficiency of study mechanism, application of the Dendrobidium huoshanness extract in enhancing immunology is researched and analysed, it is final to confirm
Based on the molecular mechanism that the enhancing for the Dendrobidium huoshanness polysaccharide for turning green group of angle is immune；The Dendrobidium huoshanness extract of the present invention leads to
Cross up-regulation α -2 and macroglobulin, macrophage activating factor (MAF) and quick stress reaction be immunized to improve the immune response ability of body,
Increase the enzyme content of glutathione by raising glutathione synthetase, reduce the oxidative damage brought by fat and high fat of blood,
So as to further alleviate the diabetic condition of diabetic.
Brief description of the drawings
Fig. 1 is the elution curve during the DEAE-52 ion column separating purification Dendrobidium huoshanness polysaccharide in embodiment 1；
Fig. 2 is the elution curve during the Sephadex G-100 column separating purification DP II components in embodiment 1；
Fig. 3 is the structure flow chart in the RNA libraries in embodiment 3.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
This example demonstrates that Dendrobidium huoshanness extract.
The acquisition process of Dendrobidium huoshanness extract is as follows：
(1) Dendrobidium huoshanness is taken to cut into slices, grind into powder after 50 DEG C of drying；80% ethanol sloughs pigment, 50 DEG C of water extraction 5h；
95% ethanol, overnight sink alcohol；60% is evaporated, and obtains Dendrobidium huoshanness Thick many candies.
(2) 2% papain, 50 DEG C of water-bath 2h, according still further to Huoshan stone are added in the Dendrobidium huoshanness Thick many candies of acquisition
Dry measure used in former times Thick many candies solution：N-butanol：Chloroform volume ratio example is 25：1：5 add reaction reagent, operate 4-5 times repeatedly, until without albumen
Layer；Then dialysed, vacuum freeze drying, obtain Dendrobidium huoshanness polysaccharide.
(3) purifying of Dendrobidium huoshanness polysaccharide
A, DEAE-52 ions column chromatography
Selection anion exchange chromatography isolates and purifies to Dendrobidium huoshanness polysaccharide, filled media DEAE-52；
The flow of DEAE-52 column chromatographies is as follows：
1. preparation
Dendrobidium huoshanness polysaccharide：0.1g polysaccharide is weighed, is dissolved in 50mL aquae destillatas, compound concentration is that 20mg/mL polysaccharide is molten
Liquid；
The preparation of gradient eluent：Prepare 0.1mol/L, 0.3mol/L, 0.5mol/L NaCl and 0.5mol/L NaOH；
2. gradient elution
DEAE-52 is pre-processed and filled into chromatographic column, starts automatic collector, is run by the program set
(1mL/min, 5min/ are managed), eluent is carried out successively from low to high according to NaCl solution concentration, finally uses 0.5mol/L instead
NaOH；
3. benzene is cruel-each test tube of sulfuric acid tracing detection in polysaccharide content, draw elution curve, as shown in figure 1, wherein,
Ordinate is OD490nm, abscissa is elution pipe number；As shown in Figure 1, the eluent being collected under three peak values, it is ordered respectively
Entitled DP I (test tube 6-16), DP II (test tube 36-41), DP III (test tube 66-71)；
4. three component DP I, DP II and DP III are carried out into cell experiment respectively, whether it is detected with hypoglycemic
Activity, wherein, DP II activity is optimal, uses it for subsequent experimental.
B, Sephadex G-100 column chromatographies
The DP II components of DEAE-52 after purification are further isolated and purified, now filled media is to be changed to
Sephadex G-100；With reference to DEAE-52 column chromatography operating procedures, it is eluent to prepare 0.05mol/L NaCl, elution speed
1mL/min, 4min/ are managed, and draw elution curve, as shown in Fig. 2 wherein, ordinate OD490nm, abscissa is elution pipe number；
As shown in Figure 2, DP II components are had into an eluting peak after Sephadex G-100 further elution；This is collected to wash
Eluent under de- peak, is concentrated into 100-200mg crude drugs/mL, filters, as final required Dendrobidium huoshanness extract.
C, constituent analysis
Constituent analysis is carried out to the Dendrobidium huoshanness extract finally obtained, according to GCMS-QP2010 gas chromatography-mass spectrometries
It can be drawn with quasi-molecular ions fragment and the monose composition drawn：The main on-link mode (OLM) of DP II components is by 1,3,4-Rha, Isosorbide-5-Nitrae-p-
Glc, 1,3,4-Glc, there are the glycosidic bond that some arabinoses and xylose form, molecular weight 48365Da.
Embodiment 2
This example demonstrates that pharmaceutical composition.
Pharmaceutical composition adds pharmaceutically acceptable load as needed using Dendrobidium huoshanness extract as main active
Body is prepared into any pharmaceutically useful formulation, including：Tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, ebonite
Wafer, soft capsule, oral liquid, mouth containing agent, granule, electuary, pill, powder, paste, sublimed preparation, supensoid agent, solution, note
Penetrate agent, suppository, ointment, emplastrum, creme, spray, drops, patch.
Wherein, pharmaceutically acceptable carrier includes but is not limited to：Mannitol, sorbierite, sorbic acid, sodium pyrosulfite,
Sodium hydrogensulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin A, vitamin C, vitamin E, dimension
Raw plain D, azone, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the carbonate of monovalence alkali metal, acetate, phosphate, hydrochloric acid, acetic acid, sulfuric acid,
Phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, shallow lake
Powder, sucrose, lactose, mannitol, silicon derivative, cellulose, alginates, gelatin, polyvinylpyrrolidone, glycerine, propane diols,
Ethanol, soil temperature 60-80, Span-80, beeswax, lanolin, atoleine, hexadecanol, agar, triethanolamine, basic amino acid,
Urea, allantoin, calcium carbonate, calcium bicarbonate, surfactant, polyethylene glycol, cyclodextrin, beta-schardinger dextrin, phospholipid material, height
Ridge soil, talcum powder, calcium stearate, magnesium stearate, vegetable oil, phosphatide, polyethylene glycol phosphatide, oleic acid, EDTA.
The pharmaceutical composition of the present embodiment, the medicament drug containing active substances 10-100mg of unit dose, remaining is pharmacy
Upper acceptable carrier, also, pharmaceutically acceptable carrier is the 0.1-99.9% of total formulation weight by weight.
The pharmaceutical composition of the present invention when in use, usage and dosage is determined according to the situation of patient, under normal circumstances, disease
People takes 50-200mg/kg (own body weight) for one day.
Embodiment 3
The present embodiment is by type II diabetes (T2DM) rat experiment and turns green group of experiment explanation Dendrobidium huoshanness extract
Application in terms of strengthening immune, regulating and controlling blood sugar and improving metabolism disorder of blood lipid.
First, type II diabetes (T2DM) rat experiment
(1) Preparatory work of experiment：56 SD male rats are SPF levels (body weight 200-250g, 8 weeks mouse ages), 22 DEG C of temperature,
Tested after adapting to week age in humidity 50 ± 10%, the 12h environment that day alternates with night.
(2) T2DM rat models are tested：
1. rat is randomly divided into two groups：(4) feeding normal diets of A groups, (52) high fat word material of feeding of B groups；After 8 weeks
Carry out the foundation of diabetes model；
2. establish diabetes model：Two groups of empty stomach 12h, B group intraperitoneal injection 25mg/Kg/d STZ solution, A groups abdominal cavity note
Penetrate citrate buffer solution；Fasting blood sugar is determined after 7d；When B groups blood glucose value is more than 11.1mmol/L, then T2DM rats are modeled as
Work(, available for follow-up experimental study.
(3) A groups rat is as blank group, and B set up the successful rat of mould be then randomly divided into 3 groups (model group, control group and
DP II groups), test process situation is as follows；
NC (blank group)：Normal SD rats, normal diet, the pure aquation of gavage；
DC (model group)：Diabetes Mellitus SD Rats, high lipid food, gavage pure water/d；
DDP II (DP II groups)：Diabetes Mellitus SD Rats, high lipid food, gavage dendrobium polysaccharide component DP II (350mg/Kg/
d)；
Continuous gavage 28 days, the fasting blood sugar of each rat is being detected on the same day and is recording changes of weight weekly, 28 days
After dissect.
2nd, green group of experiment is turned
(1) preparation and detection of Total RNA samples
Rat liver Total RNA after extraction dissection, extraction is carried out with reference to the detailed operation of kit, and is taken
0.8% agarose gel electrophoresis and Agilent Annlysis detect to Total RNA samples.
(2) structure in RNA libraries
RNA library construction test operation steps are built storehouse kit explanation according to NEB and carried out, library construction flow such as Fig. 3 institutes
Show；Qubit accurate quantitative analysis is used to the RNA libraries built, and carries out quality testing.
(3) machine is sequenced on
After library construction success, various kinds Ben Wenku mixing is carried out according to sequencing depth (lower machine data volume) first
(pooling) after, machine sequencing on HiSeq2500 is selected, this experiment sequencing service and bioinformatic analysis are by Hua Da base
Because company provides.
(4) sequencing quality is assessed
Detection is assessed to relate generally to：Error rate, A/T/G/C distributions, data filtering, the quality of data, reads are on chromosome
Density Distribution, repeat samples correlation, repeat samples gene expression etc..
(5) sequencing result quality
1. the Total RNA Library Qualities built are qualified, machine sequencing is carried out；
2. upper machine sequencing error rate is low, A/T/G/C base contentses are evenly distributed, no editing Preference, Pass Test sample
Property；
3. Cleans reads account for whole bases be respectively 98.57%, 98.50%, 98.54%97.90%；Each group parent
Q30 (%) value is all higher than 90%, and data are preferable；Reads can be compared on chromosome, and has expression in each section；Ensure
The accuracy of sequencing；
4. biological iterative testing DC group R2=0.977, DDP R2=0.975, data have preciseness.
3rd, differential genes expression analysis
Result progress differential genes expression analysis is obtained to turning green group of experiment, it is found that Dendrobidium huoshanness extract passes through enhancing
Immune response alleviates diabetic supersession syndrome with oxidative damage is improved；By promoting P13K/AKT signal paths, increase
Transhipment and utilization to glucose by force, come hypoglycemic；Improve metabolism disorder of blood lipid by influenceing lipid-metabolism signal path.Tool
Body is analyzed as follows：
(1) immune response and redox reaction analysis
Differential gene contrasts in the immune response of table 1 and redox reaction
Table 1 is differential gene contrast table in immune response and redox reaction, and its differential expression shown is predominantly exempted from
Epidemic disease response and redox reaction, while transport protein also in exogenous substance metabolism process etc..Knowable to analytical table 1,
Dendrobidium huoshanness extract being mainly shown as in the diabetes rat state of an illness is controlled：Raise α -2 and macroglobulin, macrophage is immunized
Activation factor and quick stress reaction improve the immune response ability of body；Increase paddy by raising glutathione synthetase
The enzyme content of the sweet peptide of Guang (SOD), the oxidative damage that diabetes rat is brought by fat and high fat of blood is reduced, meanwhile, synergetic immunity
Answering promotes the immunocompetence of body；In addition, the related factor of some exogenous metabolisms also be present, to regulate and control height
The toxicological effect that fat feed is brought.
It follows that Dendrobidium huoshanness polysaccharide can regulate and control by improving body oxidative damage and immune response and alleviate glycosuria
The state of an illness.
(2) the metabolic pathway analysis of glycolysis
Differential gene contrasts in the P13K/AKT signal paths of table 2
Table 2 is differential gene contrast table in P13K/AKT signal paths, and analytical table 2 understands that Dendrobidium huoshanness polysaccharide can pass through
Antidiuretic hormone is raised to alleviate " diuresis " symptom in diabetes " more drinks, diuresis, more foods "；The insulin (IN) and wing of up-regulation
Island element sample receptor protein (INR) combines, and can activate PI3K expression by raising Phosphoinoside kinase, produce NADP, stimulate Ca2+
The protein kinase of dependence, promote transhipment of the GLUT (GLUT) to glucose, reduce the storage of unnecessary glucose, drop
Low blood glucose level；And EGF can raise CAMP, enabling signal cascade reaction, promote thin by being combined with corresponding acceptor
Intracellular Ca2+Concentration rises, and increases glycolysis and the progress of protein synthesis.
It follows that Dendrobidium huoshanness polysaccharide can by activate insulin receptor downstream PI3K and other promote glycolysis
Metabolic pathway promotes the transhipment of glucose and utilization, reaches hypoglycemic effect.
(3) lipid-metabolism signal path is analyzed
Differential gene contrasts in the lipid-metabolism of table 3
Table 3 is differential gene contrast table in lipid-metabolism, and its difference expression gene shown is mainly lipid-metabolism signal
The GAP-associated protein GAP of path.Analytical table 3 understands that Dendrobidium huoshanness extract is by raising apolipoprotein L, Apolipoprotein CIII, lipid
Transport protein, abc transport albumen -1 regulate and control the utilization of lipid and whereabouts；Simultaneously raise low-density lipoprotein (LDL) acceptor and
Beta lactoglobulin improves the level of blood fat, reduces the symptom of diabetes high fat of blood；By influenceing aliphatic carboxylic acid enzyme and fat
The expression of sour desaturase regulates and controls disorders of lipid metabolism, controls problem of obesity caused by fatty excess accumulation.
It follows that Dendrobidium huoshanness polysaccharide can improve II type glycosuria obesity and blood fat generation by regulating and controlling lipid-metabolism
Xie Suluan.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. Dendrobidium huoshanness extract is in the application of enhancing immunology, it is characterised in that is specially that Dendrobidium huoshanness extract is being made
Application in standby enhancing immune drug, the medicine are used to alleviate diabetic condition；Wherein, the acquisition of the Dendrobidium huoshanness extract
Method is：
(1) Dendrobidium huoshanness is taken to cut into slices, grind into powder after 50 DEG C of drying；80% ethanol sloughs pigment, 50 DEG C of water extraction 5h；95% second
Alcohol, overnight sink alcohol；60% is evaporated, and obtains Dendrobidium huoshanness Thick many candies；
(2) take Sevage joint Papain enzyme process to take off albumen, then dialysed, vacuum freeze drying, obtain Dendrobidium huoshanness
Polysaccharide；
(3) selection anion exchange chromatography isolates and purifies to Dendrobidium huoshanness polysaccharide, collects target eluent, is concentrated into
100-200mg crude drugs/mL, filter and produce.
2. Dendrobidium huoshanness extract according to claim 1 enhancing immunology application, it is characterised in that it is described suddenly
In the obtaining step (2) of mountain Herba Dendrobii extract, take Sevage joint the Deproteinated specific method of Papain enzyme process be：Obtaining
2% papain, 50 DEG C of water-bath 2h, according still further to Dendrobidium huoshanness Thick many candies solution are added in the Dendrobidium huoshanness Thick many candies taken：Just
Butanol：Chloroform volume ratio example is 25：1：5 add reaction reagent, operate 4-5 times repeatedly, until without albumin layer.
3. Dendrobidium huoshanness extract according to claim 1 is in the application of enhancing immunology, it is characterised in that described to answer
Be by strengthen immune response and improve oxidative damage work.
4. Dendrobidium huoshanness extract according to claim 3 enhancing immunology application, it is characterised in that it is described suddenly
Macroglobulin, macrophage activating factor (MAF) and quick stress reaction is immunized to improve machine by raising α -2 in mountain Herba Dendrobii extract
The immune response ability of body.
5. Dendrobidium huoshanness extract according to claim 3 enhancing immunology application, it is characterised in that it is described suddenly
Mountain Herba Dendrobii extract increases the enzyme content of glutathione by raising glutathione synthetase, reduces because fat and high fat of blood is brought
Oxidative damage.
6. Dendrobidium huoshanness extract according to claim 1 is in the application of enhancing immunology, it is characterised in that the medicine
Thing is the pharmaceutical composition using Dendrobidium huoshanness extract as main active.
7. Dendrobidium huoshanness extract according to claim 6 is in the application of enhancing immunology, it is characterised in that the medicine
Compositions drug containing active substances 10-100mg, remaining is pharmaceutically acceptable carrier.
8. Dendrobidium huoshanness extract according to claim 7 is in the application of enhancing immunology, it is characterised in that the medicine
Acceptable carrier specifically includes on：Mannitol, sorbierite, sorbic acid, sodium pyrosulfite, sodium hydrogensulfite, thiosulfuric acid
Sodium, cysteine hydrochloride, TGA, methionine, vitamin A, vitamin C, vitamin E, vitamin D, azone, EDTA bis-
Sodium, Ethylenediaminetetraacetic Acid Calcium Salt, the carbonate of monovalence alkali metal, acetate, phosphate, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, chlorination
It is sodium, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, sweet
Reveal sugar alcohol, silicon derivative, cellulose, alginates, gelatin, polyvinylpyrrolidone, glycerine, propane diols, ethanol, soil temperature 60-80,
Span-80, beeswax, lanolin, atoleine, hexadecanol, agar, triethanolamine, basic amino acid, urea, allantoin, carbonic acid
Calcium, calcium bicarbonate, surfactant, polyethylene glycol, cyclodextrin, beta-schardinger dextrin, phospholipid material, kaolin, talcum powder, tristearin
Sour calcium, magnesium stearate, vegetable oil, phosphatide, polyethylene glycol phosphatide, oleic acid, EDTA.
9. Dendrobidium huoshanness extract according to claim 1 is in the application of enhancing immunology, it is characterised in that the medicine
Thing is any pharmaceutical dosage form.
10. Dendrobidium huoshanness extract according to claim 9 is in the application of enhancing immunology, it is characterised in that described
Pharmaceutical dosage form specifically includes：Tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule
It is agent, oral liquid, mouth containing agent, granule, electuary, pill, powder, paste, sublimed preparation, supensoid agent, solution, injection, suppository, soft
Paste, emplastrum, creme, spray, drops, patch.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN110856744A (en) * | 2018-08-24 | 2020-03-03 | 北京中医药大学 | New application of dendrobe polypeptide |
| CN114306590A (en) * | 2021-12-31 | 2022-04-12 | 皖西学院 | Veterinary vaccine diluent with immunity enhancement effect and preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110856744A (en) * | 2018-08-24 | 2020-03-03 | 北京中医药大学 | New application of dendrobe polypeptide |
| CN110856744B (en) * | 2018-08-24 | 2022-11-01 | 北京中医药大学 | New application of dendrobe polypeptide |
| CN108912236A (en) * | 2018-09-29 | 2018-11-30 | 霍山县天下泽雨生物科技发展有限公司 | A kind of Dendrobidium huoshanness refined polysaccharide and preparation method thereof with antitumor action |
| CN110403906A (en) * | 2019-09-05 | 2019-11-05 | 皖西学院 | A kind of preparation method of Dendrobidium huoshanness polyoses injection |
| CN114306590A (en) * | 2021-12-31 | 2022-04-12 | 皖西学院 | Veterinary vaccine diluent with immunity enhancement effect and preparation method and application thereof |
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