CN107607608A - A kind of Single cell analysis method - Google Patents
A kind of Single cell analysis method Download PDFInfo
- Publication number
- CN107607608A CN107607608A CN201710806348.3A CN201710806348A CN107607608A CN 107607608 A CN107607608 A CN 107607608A CN 201710806348 A CN201710806348 A CN 201710806348A CN 107607608 A CN107607608 A CN 107607608A
- Authority
- CN
- China
- Prior art keywords
- syringe
- microchannel
- constant current
- analysis method
- cell sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention belongs to detection technique field, discloses a kind of Single cell analysis method, comprises the following steps：Syringe is driven by constant current syringe pump, cell sample and organic reagent are directed respectively into micro-fluidic chip；The cell sample and the organic reagent form microlayer model after entering the micro-fluidic chip；The microlayer model enters icp mses, and the icp mses carry out analysis detection using time-resolved mode to the microlayer model.The present invention solves carries out time resolution analysis using icp mses conventional soln nebulization sampling pattern to the element in cell in the prior art, whether each jump peak-to-peak signal obtained by can not determining is single celled signal, the problem of so as to cause testing result not accurate enough.This invention ensures that detection signal comes from unicellular, effectively analysis of the realization to unicellular middle constituent content.
Description
Technical field
The present invention relates to detection technique field, more particularly to a kind of Single cell analysis method.
Background technology
With deepening continuously for biochemical research, increasing research show cell it is heterogeneous be widely present and
It can produce different influences to the physiology course of cell.By taking cancer cell as an example, the heterogeneous performance of cell by random gene and
It is apparent to change the phenotype for changing cancer cell and function (clone evolves) so as to change the oncogenicity of cell.Based on this, development is easy
Efficient method is used for Single cell analysis tool and is of great significance.
Elemental Speciation Analysis is combined real typically by efficient isolation technics with highly sensitive element specific detection technology
It is existing.In many element specific detection devices, icp mses (ICP-MS) have prominent advantage, no
Only high sensitivity, detection limit are low, the range of linearity is wide, and can also provide isotope relevant information.The wherein quadrupole rod of commercialization
ICP-MS costs are most cheap, popularization degree highest, and the assay of unicellular middle object element can be realized based on time-resolved mode.
Prior art generally use ICP-MS conventional soln nebulization sampling patterns carry out time resolution analysis to the trace element in cell,
But because the formation of aerosol is random, each jump peak-to-peak signal obtained by can not determining is individual cells or multiple
The signal of cell.Therefore, although the probability that multiple cells are determined in time resolution ICP-MS is relatively low, experimental result can still be caused
Inaccuracy.
The content of the invention
The embodiment of the present application is solved in the prior art using inductive etc. by providing a kind of Single cell analysis method
Gas ions mass spectrograph conventional soln nebulization sampling pattern carries out time resolution analysis to the element in cell, obtained by can not determining
Each jump peak-to-peak signal whether be single celled signal, the problem of so as to cause testing result not accurate enough.
The embodiment of the present application provides a kind of Single cell analysis method, comprises the following steps：
Syringe is driven by constant current syringe pump, cell sample and organic reagent are directed respectively into micro-fluidic chip；
The cell sample and the organic reagent form microlayer model after entering the micro-fluidic chip；
The microlayer model enters icp mses, when the icp mses use
Between differentiate pattern analysis detection is carried out to the microlayer model.
Preferably, the constant current syringe pump includes the first organic phase constant current syringe pump, cell sample constant current syringe pump, second
Organic phase constant current syringe pump；The syringe includes the first syringe, the second syringe, the 3rd syringe；
Being drawn in first syringe and the 3rd syringe has the organic reagent, is inhaled in second syringe
The cell sample is taken；
The first organic phase constant current syringe pump drives first syringe, the cell sample constant current syringe pump driving
Second syringe, the Second Organic Phase constant current syringe pump drive the 3rd syringe.
Preferably, the micro-fluidic chip is provided with cross focus type passage, and it is micro- that the cross focus type passage includes first
Passage, the second microchannel, the 3rd microchannel, the 4th microchannel；Described in first microchannel and the 3rd microchannel are formed
The vertical passage of cross focus type passage, second microchannel and the 4th microchannel form the cross focus type passage
Interconnection；
One end of first microchannel is provided with the first organic phase sample introduction entrance, and one end of second microchannel is provided with carefully
Born of the same parents' sample feeding entrance, one end of the 3rd microchannel are provided with Second Organic Phase sample introduction entrance, and the one of the 4th microchannel
End is provided with outlet；
Organic reagent in first syringe imports the first organic phase sample introduction entrance by pump line, and described second
The cell sample in syringe imports the cell sample sample introduction entrance by pump line, organic in the 3rd syringe
Reagent imports the Second Organic Phase sample introduction entrance by pump line.
Preferably, the exit is fixed with quartz capillary, the quartzy capillary by cyanacrylate sealing
The other end of pipe is fixed on the icp mses by PEEK pipes, and the microlayer model of formation passes through institute
State quartz capillary and import the icp mses.
Preferably, the icp mses are analyzed the microlayer model using time-resolved mode
Detection, sampling interval 1-5ms.
Preferably, the flow velocity of the cell sample is 5-8 μ L min-1, the flow velocity of the organic reagent is 12.5-20 μ L
min-1。
Preferably, the density of the cell sample is 5.0 × 105mL-1。
Preferably, the organic reagent is hexanol.
The one or more technical schemes provided in the embodiment of the present application, have at least the following technical effects or advantages：
In the embodiment of the present application, syringe is driven by constant current syringe pump, cell sample and organic reagent is led respectively
Enter micro-fluidic chip；Cell sample and organic reagent form microlayer model after entering micro-fluidic chip；Microlayer model enters inductive
Plasma mass spectrograph, icp mses carry out analysis detection using time-resolved mode to microlayer model.This
Invention, using drop as unicellular carrier, can effectively separate using micro-fluidic chip as drop formation and the platform of control
It is unicellular.The present invention uses micro-fluidic chip and inductively coupled plasma mass spectrometer coupling, ensure that detection signal comes from list
Cell, effectively realize the analysis to unicellular middle constituent content.
Brief description of the drawings
It is required in being described below to embodiment to use in order to illustrate more clearly of the technical scheme in the present embodiment
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are one embodiment of the present of invention, for this area
For those of ordinary skill, on the premise of not paying creative work, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is a kind of flow chart of Single cell analysis method provided in an embodiment of the present invention；
Fig. 2 is the structural representation for the device that a kind of Single cell analysis method provided in an embodiment of the present invention uses.
Wherein, 10- the first organic phase constant currents syringe pump, the syringes of 11- first, 20- cell sample constant currents syringe pump, 21-
Second syringe, 30- Second Organic Phase constant currents syringe pump, the syringes of 31- the 3rd, 40- the first organic phase sample introductions entrance, 50- are thin
Born of the same parents' sample feeding entrance, 60- Second Organic Phase sample introductions entrance, 70- outlets；
100- micro-fluidic chips, 200- icp mses.
Embodiment
The embodiment of the present application is solved in the prior art using inductive etc. by providing a kind of Single cell analysis method
Gas ions mass spectrograph conventional soln nebulization sampling pattern carries out time resolution analysis to the element in cell, obtained by can not determining
Each jump peak-to-peak signal whether be single celled signal, the problem of so as to cause testing result not accurate enough.
The technical scheme of the embodiment of the present application is in order to solve the above technical problems, general thought is as follows：
A kind of Single cell analysis method, comprises the following steps：
Syringe is driven by constant current syringe pump, cell sample and organic reagent are directed respectively into micro-fluidic chip；
The cell sample and the organic reagent form microlayer model after entering the micro-fluidic chip；
The microlayer model enters icp mses, when the icp mses use
Between differentiate pattern analysis detection is carried out to the microlayer model.
The present invention is using micro-fluidic chip as drop formation and the platform of control, using drop as unicellular carrier, energy
Enough effectively separate unicellular.The present invention uses micro-fluidic chip and inductively coupled plasma mass spectrometer coupling, ensure that detection letter
Number come from unicellular, effectively realize the analysis to unicellular middle constituent content.
In order to be better understood from above-mentioned technical proposal, below in conjunction with Figure of description and specific embodiment to upper
Technical scheme is stated to be described in detail.
A kind of Single cell analysis method is present embodiments provided, as shown in figure 1, comprising the following steps：
Step 10：Syringe is driven by constant current syringe pump, cell sample and organic reagent are directed respectively into micro-fluidic core
Piece.
The structural representation of the device that a kind of Single cell analysis method provided in an embodiment of the present invention uses as shown in Fig. 2
Including：Micro-fluidic chip 100, icp mses 200, constant current syringe pump, syringe.
The constant current syringe pump includes the first organic phase constant current syringe pump 10, cell sample constant current syringe pump 20, second has
Machine phase constant current syringe pump 30；The syringe includes the first syringe 11, the second syringe 21, the 3rd syringe 31.
Being drawn in first syringe 11 and the 3rd syringe 31 has the organic reagent, second syringe
Being drawn in 21 has the cell sample.
The first organic phase constant current syringe pump 10 drives first syringe 11, the cell sample constant current syringe pump
20 driving second syringes 21, the Second Organic Phase constant current syringe pump 30 drive the 3rd syringe 31.
The micro-fluidic chip 100 is provided with cross focus type passage, the cross focus type passage include the first microchannel,
Second microchannel, the 3rd microchannel, the 4th microchannel；First microchannel and the 3rd microchannel form the cross and gathered
The vertical passage of burnt type passage, second microchannel and the 4th microchannel form the transverse direction of the cross focus type passage
Passage.
One end of first microchannel is provided with the first organic phase sample introduction entrance 40, and one end of second microchannel is provided with
Cell sample sample introduction entrance 50, one end of the 3rd microchannel are provided with Second Organic Phase sample introduction entrance 60, and the described 4th is micro- logical
The one end in road is provided with outlet 70.
Organic reagent in first syringe 11 imports the first organic phase sample introduction entrance 40 by pump line, described
The cell sample in second syringe 21 imports the cell sample sample introduction entrance 50, the 3rd syringe by pump line
Organic reagent in 31 imports the Second Organic Phase sample introduction entrance 60 by pump line.The pump line is preferably polyethylene pump line.
The flow velocity of the cell sample is 5-8 μ L min-1, the flow velocity of the organic reagent is 12.5-20 μ L min-1。
The density of the cell sample is 5.0 × 105mL-1。
The organic reagent is hexanol.The preferably hexanol containing 1%Span80.
Preferable situation, first microchannel, second microchannel, the width of the 3rd microchannel are 200 μm,
The width of 4th microchannel is 150 μm；It is first microchannel, second microchannel, the 3rd microchannel, described
The height of 4th microchannel is 50 μm.Width at the right-angled intersection of the cross focus type passage is 75 μm.The longitudinal direction leads to
The total length in road is
4mm, the total length of the interconnection is 5mm.
Wherein：Constant current syringe pump is TS2-60 type constant currents syringe pump (Baoding LanGe constant flow pump Co., Ltd, China), is injected
Device is 1mL (Shanghai Jinta Medical Equipment Co., Ltd., China).Icp mses are Xseries types ICP-
MS(Thermo,USA)。
The processing method of PDMS micro-fluidic chips：The making of silicon template uses soft lithography process, uses AZ-50XT photoetching
Glue.Wherein, fluid passage preparation method：By GE RTV 615 (PDMS) component A (performed polymer) and B component (curing agent) with matter
Measure ratio 10:1 mixing, stirs, is placed in vacuum desiccator and vacuumizes 10min using oil pump, static after taking-up to treat that bubble disappears
Lose.PDMS colloidal sols are cast on silicon masterplate, 75 DEG C of solidification 3h, the PDMS of solidification peeled off from silicon masterplate, in feeder connection
Punched with the port of export.The preparation method of PDMS chips：The PDMS processed and slide are positioned in plasma cleaner, used
Oxygen plasma handles 1min, is bonded rapidly after taking-up, and then 75 DEG C of solidification 10min make the complete aging of bonding face.
Step 20：Cell sample and organic reagent form microlayer model after entering micro-fluidic chip.
Using the micro-fluidic chip 100 as drop formation and the platform of control, using drop as unicellular carrier, energy
Enough effectively separate unicellular.
Step 30：Microlayer model enters icp mses, when icp mses use
Between differentiate pattern analysis detection is carried out to the microlayer model.
Quartz capillary is fixed with by cyanacrylate sealing at the outlet 70, the quartz capillary it is another
One end is fixed on the icp mses 200 by PEEK pipes, and the microlayer model of formation passes through described
Quartz capillary imports the icp mses 200.
Preferable situation, a diameter of 75 μm of the quartz capillary, a length of 3cm.
Cell sample is 5.0 × 105mL-1HepG2 cell suspending liquids.
The icp mses 200 carry out analysis inspection using time-resolved mode to the microlayer model
Survey, sampling interval 1-5ms.
The Zn-ef ficiency content in single HepG2 cells is detected in optimal conditions, its average signal strength is
17429CPS, using 222nm ZnO nanoparticle in Single cell analysis device corresponding to method provided by the invention to single
Zn-ef ficiency content carries out quantitative analysis in nano-particle, and its average signal strength is 20653CPS.By ZnO nanoparticle to list
Zn-ef ficiency content carries out quantitative analysis in individual cell, the results showed that, it is 2.0 × 10 that zinc atom number is contained in individual cells8(etc.
It is same as 21.7fg).In order to verify the accuracy of resulting result, the acid resolution icp mses of routine are used
Detection is measured to the Zn content in cell sample, and measurement result shows that the average zinc atom number in HepG2 cells is 2.3
×108(being equal to 25.0fg), it is consistent with the result of the inventive method detection gained.
A kind of Single cell analysis method provided in an embodiment of the present invention comprises at least following technique effect：
In the embodiment of the present application, syringe is driven by constant current syringe pump, cell sample and organic reagent is led respectively
Enter micro-fluidic chip；Cell sample and organic reagent form microlayer model after entering micro-fluidic chip；Microlayer model enters inductive
Plasma mass spectrograph, icp mses carry out analysis detection using time-resolved mode to microlayer model.This
Invention, using drop as unicellular carrier, can effectively separate using micro-fluidic chip as drop formation and the platform of control
It is unicellular.The present invention uses micro-fluidic chip and inductively coupled plasma mass spectrometer coupling, ensure that detection signal comes from list
Cell, effectively realize the analysis to unicellular middle constituent content.
It should be noted last that above embodiment is merely illustrative of the technical solution of the present invention and unrestricted,
Although the present invention is described in detail with reference to example, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent substitution, without departing from the spirit and scope of technical solution of the present invention, it all should cover
Among scope of the presently claimed invention.
Claims (8)
- A kind of 1. Single cell analysis method, it is characterised in that comprise the following steps：Syringe is driven by constant current syringe pump, cell sample and organic reagent are directed respectively into micro-fluidic chip；The cell sample and the organic reagent form microlayer model after entering the micro-fluidic chip；The microlayer model enters icp mses, and the icp mses are using the time point Distinguish that pattern carries out analysis detection to the microlayer model.
- 2. Single cell analysis method according to claim 1, it is characterised in that it is organic that the constant current syringe pump includes first Phase constant current syringe pump, cell sample constant current syringe pump, Second Organic Phase constant current syringe pump；The syringe includes the first injection Device, the second syringe, the 3rd syringe；Being drawn in first syringe and the 3rd syringe has the organic reagent, and being drawn in second syringe has The cell sample；The first organic phase constant current syringe pump drives first syringe, described in the cell sample constant current syringe pump driving Second syringe, the Second Organic Phase constant current syringe pump drive the 3rd syringe.
- 3. Single cell analysis method according to claim 2, it is characterised in that the micro-fluidic chip focuses on provided with cross Type passage, the cross focus type passage include the first microchannel, the second microchannel, the 3rd microchannel, the 4th microchannel；It is described First microchannel and the 3rd microchannel form the vertical passage of the cross focus type passage, second microchannel and institute State the interconnection that the 4th microchannel forms the cross focus type passage；One end of first microchannel is provided with the first organic phase sample introduction entrance, and one end of second microchannel is provided with cell sample Product sample introduction entrance, one end of the 3rd microchannel are provided with Second Organic Phase sample introduction entrance, and one end of the 4th microchannel is set There is outlet；Organic reagent in first syringe imports the first organic phase sample introduction entrance, second injection by pump line The cell sample in device imports the cell sample sample introduction entrance by pump line, the organic reagent in the 3rd syringe The Second Organic Phase sample introduction entrance is imported by pump line.
- 4. Single cell analysis method according to claim 3, it is characterised in that the exit passes through alpha-cyanoacrylate second Ester sealing is fixed with quartz capillary, the other end of the quartz capillary by PEEK pipes be fixed on described inductive etc. from On daughter mass spectrograph, the microlayer model of formation imports the inductivity coupled plasma mass spectrometry by the quartz capillary Instrument.
- 5. Single cell analysis method according to claim 1, it is characterised in that the icp mses Analysis detection, sampling interval 1-5ms are carried out to the microlayer model using time-resolved mode.
- 6. Single cell analysis method according to claim 1, it is characterised in that the flow velocity of the cell sample is 5-8 μ L min-1, the flow velocity of the organic reagent is 12.5-20 μ L min-1。
- 7. Single cell analysis method according to claim 1, it is characterised in that the density of the cell sample be 5.0 × 105mL-1。
- 8. Single cell analysis method according to claim 1, it is characterised in that the organic reagent is hexanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710806348.3A CN107607608A (en) | 2017-09-08 | 2017-09-08 | A kind of Single cell analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710806348.3A CN107607608A (en) | 2017-09-08 | 2017-09-08 | A kind of Single cell analysis method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107607608A true CN107607608A (en) | 2018-01-19 |
Family
ID=61063398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710806348.3A Pending CN107607608A (en) | 2017-09-08 | 2017-09-08 | A kind of Single cell analysis method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107607608A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108579829A (en) * | 2018-04-25 | 2018-09-28 | 合肥工业大学 | Exempt from pump type micro-fluidic chip and preparation method thereof and portable biochemical analyser |
| CN109540771A (en) * | 2018-12-18 | 2019-03-29 | 武汉大学 | A kind of the acousto-optic micro-fluidic chip and its method for separating of accurate sorting leukocyte sub-type |
| CN109613105A (en) * | 2019-01-04 | 2019-04-12 | 东北大学 | A kind of unicellular drop/mono- drop generating system and its application method |
| CN110208360A (en) * | 2018-02-28 | 2019-09-06 | 桂林电子科技大学 | The unicellular mass spectrograph of light power resonant mode and unicellular mass spectrum preparation method |
| CN110687188A (en) * | 2019-09-29 | 2020-01-14 | 东北大学 | Micro-fluidic chip mass spectrometry system for single cell analysis and application method thereof |
| CN112014456A (en) * | 2020-08-28 | 2020-12-01 | 中检集团南方测试股份有限公司 | High-precision drug detection and analysis system |
| WO2021120451A1 (en) * | 2019-12-17 | 2021-06-24 | 北京大学 | Flow cytometric analysis technique for organic mass spectrometry |
| CN113237942A (en) * | 2021-05-07 | 2021-08-10 | 上海科技大学 | Method for detecting multiple trace elements in trace cells and application |
| CN114602564A (en) * | 2022-03-04 | 2022-06-10 | 广东省科学院生物与医学工程研究所 | Droplet microfluidic system and control method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439241A (en) * | 2013-08-23 | 2013-12-11 | 东南大学 | Micro-fluidic chip detection system based on single-cell multi-parameter representation |
| CN103926190A (en) * | 2014-05-08 | 2014-07-16 | 齐鲁工业大学 | Automatic single cell analysis method based on microfluidic system |
| CN203929785U (en) * | 2014-05-08 | 2014-11-05 | 齐鲁工业大学 | The unicellular automatic analysing apparatus of micro-fluidic chip based on double light path |
| CN104388300A (en) * | 2014-11-27 | 2015-03-04 | 山东师范大学 | Micro-fluidic continuous sampling method and device for unicellular quantitative analysis |
| CN204330675U (en) * | 2014-12-19 | 2015-05-13 | 中国工程物理研究院材料研究所 | The combined apparatus of a kind of two-phase laminar flow microchip and inductivity coupled plasma mass spectrometry |
| CN205484232U (en) * | 2016-03-23 | 2016-08-17 | 武汉大学 | Micro -fluidic chip and little extraction system of online automatic chip magnetism solid phase |
| CN105944775A (en) * | 2016-06-22 | 2016-09-21 | 苏州汶颢芯片科技有限公司 | Micro-fluidic chip for single-cell isolation |
-
2017
- 2017-09-08 CN CN201710806348.3A patent/CN107607608A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439241A (en) * | 2013-08-23 | 2013-12-11 | 东南大学 | Micro-fluidic chip detection system based on single-cell multi-parameter representation |
| CN103926190A (en) * | 2014-05-08 | 2014-07-16 | 齐鲁工业大学 | Automatic single cell analysis method based on microfluidic system |
| CN203929785U (en) * | 2014-05-08 | 2014-11-05 | 齐鲁工业大学 | The unicellular automatic analysing apparatus of micro-fluidic chip based on double light path |
| CN104388300A (en) * | 2014-11-27 | 2015-03-04 | 山东师范大学 | Micro-fluidic continuous sampling method and device for unicellular quantitative analysis |
| CN204330675U (en) * | 2014-12-19 | 2015-05-13 | 中国工程物理研究院材料研究所 | The combined apparatus of a kind of two-phase laminar flow microchip and inductivity coupled plasma mass spectrometry |
| CN205484232U (en) * | 2016-03-23 | 2016-08-17 | 武汉大学 | Micro -fluidic chip and little extraction system of online automatic chip magnetism solid phase |
| CN105944775A (en) * | 2016-06-22 | 2016-09-21 | 苏州汶颢芯片科技有限公司 | Micro-fluidic chip for single-cell isolation |
Non-Patent Citations (5)
| Title |
|---|
| MUELLER L,ET AL: "Trends in single-cell analysis by use of ICP-MS", 《ANALYTICAL AND BIOANALYTICAL CHEMISTRY》 * |
| TSANG,ET AL: "Tracking Bismuth Antiulcer Drug Uptake in Single Helicobacter pylori cells", 《J AM CHEM SOC》 * |
| WANG,ET AL: "Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells", 《ANALYST》 * |
| WEI,ET AL: "Analysis of the Distribution Pattern of Chromium Species in Single Cells", 《ANAL CHEM》 * |
| ZHANG J,ET AL: "Chip-based monolithic microextraction combined with ICP-MS for the determination of bismuth in HepG2 cells", 《JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208360A (en) * | 2018-02-28 | 2019-09-06 | 桂林电子科技大学 | The unicellular mass spectrograph of light power resonant mode and unicellular mass spectrum preparation method |
| CN108579829B (en) * | 2018-04-25 | 2020-02-11 | 合肥工业大学 | Pump-free micro-fluidic chip, preparation method thereof and portable biochemical analysis device |
| CN108579829A (en) * | 2018-04-25 | 2018-09-28 | 合肥工业大学 | Exempt from pump type micro-fluidic chip and preparation method thereof and portable biochemical analyser |
| CN109540771A (en) * | 2018-12-18 | 2019-03-29 | 武汉大学 | A kind of the acousto-optic micro-fluidic chip and its method for separating of accurate sorting leukocyte sub-type |
| CN109613105A (en) * | 2019-01-04 | 2019-04-12 | 东北大学 | A kind of unicellular drop/mono- drop generating system and its application method |
| CN110687188B (en) * | 2019-09-29 | 2021-05-18 | 东北大学 | Micro-fluidic chip mass spectrometry system for single cell analysis and application method thereof |
| CN110687188A (en) * | 2019-09-29 | 2020-01-14 | 东北大学 | Micro-fluidic chip mass spectrometry system for single cell analysis and application method thereof |
| WO2021120451A1 (en) * | 2019-12-17 | 2021-06-24 | 北京大学 | Flow cytometric analysis technique for organic mass spectrometry |
| CN112014456B (en) * | 2020-08-28 | 2021-04-09 | 中检集团南方测试股份有限公司 | High-precision drug detection and analysis system |
| CN112014456A (en) * | 2020-08-28 | 2020-12-01 | 中检集团南方测试股份有限公司 | High-precision drug detection and analysis system |
| CN113237942A (en) * | 2021-05-07 | 2021-08-10 | 上海科技大学 | Method for detecting multiple trace elements in trace cells and application |
| CN113237942B (en) * | 2021-05-07 | 2023-09-26 | 上海科技大学 | Method for detecting multiple microelements in microelements and application thereof |
| CN114602564A (en) * | 2022-03-04 | 2022-06-10 | 广东省科学院生物与医学工程研究所 | Droplet microfluidic system and control method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107607608A (en) | A kind of Single cell analysis method | |
| CN207096166U (en) | A kind of system for Single cell analysis | |
| EP2191895B1 (en) | Microparticle analysis device, microfluidic chip for microparticle analysis, and microparticle analysis method | |
| CN104931440B (en) | A kind of Portable heavy metal high-sensitivity detection device based on micro-fluidic chip | |
| CN105388309B (en) | The automatic quick determination method of trace iron ions and system and application in Power Plant Water Vapor | |
| CN103323502B (en) | Micro-fluidic chip detection system for flow detection | |
| CN102725613A (en) | Molecule detection device formed in a semiconductor structure | |
| CN109590038B (en) | Submicron flow channel micro-fluidic chip and manufacturing method thereof | |
| CN203220910U (en) | Integrated chip for high-throughput sorting and count detection of biological particles | |
| CN102764677B (en) | Preparation method of localized surface plasmon resonance (LSPR) microfluidic chip | |
| KR20130045236A (en) | Microchip and particulate analyzing device | |
| CN208766081U (en) | High throughput micro-scale automatic sample handling system | |
| CN106644900A (en) | Pulse impedance particle counting device based on non-uniform electric field and particle counting method | |
| CN105021578A (en) | Fluid fluorescence quantitative detection apparatus and fluid fluorescence quantitative detection method | |
| CN110426445A (en) | Three-dimensional order spiral focuses nanoparticle/single cell analysis system and application method | |
| CA3019181C (en) | Dmf method and system for concentrating analyte from large volumes into smaller volumes using magnetic microparticles | |
| CN208795661U (en) | A kind of chemiluminescence micro-fluidic chip and the analysis instrument containing it | |
| CN104307583B (en) | Chip-scale gas-liquid separator and control system thereof | |
| CN205080143U (en) | Automatic quick detecting system of trace iron ion in power plant's steam | |
| CN204241366U (en) | A kind of device detecting heavy metal in soil | |
| CN108212228B (en) | Whole blood separation micro-fluidic chip, detection device and whole blood detection method | |
| CN201508363U (en) | A device for eliminating fine bubble from water in a total phosphorus analyzing flowing system | |
| CN110506201B (en) | Particle detection device and particle detection method | |
| CN105728071A (en) | Microfluidic chip and application thereof | |
| CN206489050U (en) | A kind of impedance pulse grain count device based on inhomogeneous field |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180119 |
|
| RJ01 | Rejection of invention patent application after publication |