CN107603913A - A kind of mixed bacterial and its application in removing iron by kaolin brightens - Google Patents
A kind of mixed bacterial and its application in removing iron by kaolin brightens Download PDFInfo
- Publication number
- CN107603913A CN107603913A CN201710997647.XA CN201710997647A CN107603913A CN 107603913 A CN107603913 A CN 107603913A CN 201710997647 A CN201710997647 A CN 201710997647A CN 107603913 A CN107603913 A CN 107603913A
- Authority
- CN
- China
- Prior art keywords
- iron
- bacterium
- mixed bacterial
- kaolin
- reducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention discloses a kind of mixed bacterial and its application in removing iron by kaolin brightens, and the flora includes iron-reducing bacterium RBFL, iron-reducing bacterium RBFL3, iron-reducing bacterium FeRB FL1404, iron-reducing bacterium FL H1, the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol, achromobacter, Geobacter bremensis Straub and Buchholz Cleven；Due to the synergy between microbial strains in mixed bacterial, microorganism can be improved to kaolinic de-ferrous effect.Mixed bacterial culture is obtained bacterium solution and is used to go back Fe in protokaolin by the present invention2O3, make insoluble Fe2O3It is reduced into water-soluble Fe2+Removed by separation of solid and liquid, reduce Fe in kaolin2O3Content, improve kaolin whiteness.The present invention is low with cost, environment-friendly, and the not high low-grade kaolinite ore of script extraction value can be enable to exploit, and extends mine life, realizes low-grade kaolin high-qualityization.
Description
Technical field
The invention belongs to kaolin processing technique field, and in particular to a kind of mixed bacterial, for going back protokaolin Fe203,
Make Fe insoluble in kaolin203It is changed into solvable Fe2+, reduce Fe in kaolin203Content, so as to improve kaolin whiteness,
Lift kaolinic commercial value.
Background technology
Kaolin is a kind of nonmetallic mineral material with excellent processing performance and significant application value, is widely used in
The field of industrial production such as ceramics, papermaking, building materials, chemical industry.China's kaolin ore aboundresources, about 3,000,000,000 tons of proven reserve, position
Occupy third place in the world.Kaolin is also one of the Dominant mineral in Fujian, accounts for the 10% of national kaolin reserves, wherein 80% with
Upper is high ferro kaolin, and grade is low, it is difficult to utilizes.Whiteness is one of important indicator for determining kaolin application value, according to it
Purposes is different, there is different requirements to its whiteness, and such as in paper industry, whiteness is required to coating level kaolin>83%, contain
Fe203<0.5%；In ceramic industry, it is desirable to whiteness 80%-85%；Make high-grade porcelain ingredient requirement and contain Fe203<0.5%.It is however, more
The not purified kaolin whiteness of number is below this value, and this is due to the kaolin in nature, is often influenceed containing coloring impurity
Its whiteness, these kaolin are made to cannot be used for industry, this not only has a strong impact on the development and the utilization of resources of kaolin industry, simultaneously
It will also result in huge economic loss.Fe is one of kaolinic main dyeing element, and the kaoline deposit of the various origin causes of formation all contains
There is iron, during kaolin is formed, iron oxide deposits are in ore deposit, and majority is with Fe203Form preservation in mineral grain.By
In Fe203Have a significant impact to kaolinic whiteness, so as to influence its commercial value, therefore be stand in the breach should be eliminated it is miscellaneous
Matter composition.The removing of di-iron trioxide in kaolin, it is considered as a problem always in ore dressing circle.Particularly particle diameter is less than 10 μm
Iron Oxide Minerals, because its particle diameter is tiny and occurrence form is complicated, to it is kaolinic purification bring difficulty.
Improve kaolin whiteness conventional method have Physical (including sorting, wet cleaning process, magnetic method, floatation etc.) and
Chemical method (including oxidizing process, reducing process, oxidation-reduction method etc.), however, these methods disappear in the prevalence of processing procedure energy
The shortcomings of consumption is big, running cost is high and causes environmental pollution, removing iron by kaolin increasing is carried out especially with the chemical agent of costliness
In vain, its operating cost and the cost of raw material are higher, caused by environmental pollution it is also more serious.
Mineral biology (predominantly bacterium) process technology is an emerging mineral process technology, and its distinguishing feature is investment
Less, cost is low, energy consumption is small, environment-friendly.The present invention uses mixed bacterial also protokaolin Fe203, make insoluble in kaolin
Fe203It is changed into solvable Fe2+, then removed by separation of solid and liquid, reduce Fe in kaolin203Content, it is white to improve kaolin
Degree, at home and abroad lifting kaolin product value and its competitiveness in market, realizes kaolinic deep processing and economic value
Raising.Kaolin bacterium iron-removal and whitening technology has its unique advantage, is got in mineral resources increasingly poor and environmental requirement
Come it is stricter in the case of, the low-grade kaolinite ore that script extraction value can be enable not high is exploited, extend mine service year
Limit, realizes comprehensive utilization of resources and sustainable development, will will produce significant economic, society and environmental benefit.
Iron tramp in the removal kaolin that the present invention is a kind of environment-friendly type, handling process is simple and convenient to operate, cost is low
Method, due to the synergy between microbial strains in mixed bacterial, microorganism is improved to kaolinic de-ferrous effect.
The content of the invention
It is an object of the invention to provide a kind of mixed bacterial and its application in removing iron by kaolin brightens, the mixed bacterial
By going back Fe in protokaolin2O3To reduce Fe in kaolin2O3Content, so as to improve kaolin whiteness, lift kaolinic business
Industry is worth, i.e., particular kind of microorganism is expanded to the bacterium solution cultivated and obtained, is added to the nutrients needed for containing the microorganism chief
（Carbon source, nitrogen source）Kaolinite ore pulp soil in, carry out iron removaling reaction treatment 8-15 days.
The mixed bacterial of the present invention includes iron-reducing bacterium RBFL ((Bacillus pumilus, bacillus brevis), iron
Reducing bacteria RBFL3 (Bacillus pumilus, bacillus pumilus), iron-reducing bacterium FeRB-FL1404 (Bacillus
Pumilus, bacillus pumilus), iron-reducing bacterium FL-H1, the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）, achromobacter
（CCTCC AB 206571）、Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-
607）Viable bacteria, its number of viable ratio be 3：4-7：3-6：5-9：1：2：2-5.Wherein：
（1）Iron-reducing bacterium RBFL, iron-reducing bacterium FL-H1, iron-reducing bacterium RBFL3 and iron-reducing bacterium FeRB-FL1404 are by application
Person is isolated from Kaoline mine（See documents）；
（2）The hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol can be from Chinese agriculture Microbiological Culture Collection administrative center（ACCC）Purchase, preserving number are
ACCC 00386；
（3）Achromobacter can be from China typical culture collection center（CCTCC）Purchase, preserving number is CCTCC AB 206571；
（4）Geobacter bremensis Straub and Buchholz-Cleven can be from American Type Culture collection warehousing
（ATCC) buy, preserving number is ATCC BAA-607.
The application of described mixed bacterial, water is added to be configured to 10-25% concentration for the kaolin of 200-400 mesh particle diameter
Ore pulp A；
The carbon source of 1.0-2.5% ore pulp quality, the nitrogen source of 1.0-2.5% ore pulp quality degree, 0.005- are added in above-mentioned ore pulp A
The inorganic salts of the surfactant of 0.01% ore pulp quality and 0.005~0.01% ore pulp quality, are configured to ore pulp B；
The bacterium solution of mixed bacterial culture is added in ore pulp B, dosage is ore pulp quality A 10-15%, and bacterial concentration is about
107CFU/ml-109CFU/ml, it is configured to ore pulp C；
Ore pulp C is carried out into iron removaling in 15-40 DEG C to react 8-15 days；
By reacted kaolin slurry through separation of solid and liquid, produce and remove Fe2O3Iron tramp kaolin, through 105 DEG C of dryings
After determine its whiteness and Fe2O3Content.
The mixed bacterial that the present invention is formed using the various combination of this 7 kinds of bacterium is applied in removing iron by kaolin whitening,
By Mixed Microbes to Fe insoluble in kaolin2O3Water-soluble ferrous ion is reduced into, then by separation of solid and liquid, makes height
Ridge soil Fe2O3Content reduces, and improves kaolin whiteness.
The object of the present invention is achieved like this, and one kind goes back Fe in protokaolin2O3Mixed bacterial, it is characterised in that institute
Selected from iron-reducing bacterium RBFL, ((Bacillus pumilus, bacillus brevis), iron reduce at least two kinds of the mixed bacterial stated
Bacterium RBFL3 (Bacillus pumilus, bacillus pumilus), iron-reducing bacterium FeRB-FL1404 (Bacillus
Pumilus, bacillus pumilus), iron-reducing bacterium FL-H1, the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）, achromobacter
（CCTCC AB 206571）、Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-
607）, each bacterium is activated respectively and expansion culture obtains corresponding bacterium solution, and viable bacteria concentration is 10 in respective bacterium solution7 CFU/ml -
109CFU/ml, described iron-reducing bacterium RBFL ((Bacillus pumilus, bacillus brevis)：Iron-reducing bacterium RBFL3
(Bacillus pumilus, bacillus pumilus)：Iron-reducing bacterium FeRB-FL1404 (Bacillus pumilus, short and small bud
Spore bacillus)：Iron-reducing bacterium FL-H1：The hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）：Achromobacter（CCTCC AB 206571）：
Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-607）Number of viable ratio be 3：
4-7：3-6：5-9：1：2：2-5.
The concentration of bacterium is 10 in described mixed bacterial7 CFU/ml -109 CFU/ml。
Above-mentioned bacterium solution presses the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）And achromobacter（CCTCC AB 206571）Body
Product is than being 1：2 are configured to the bacterium solution of mixed bacterial.
Above-mentioned bacterium solution is 1 by iron-reducing bacterium RBFL, iron-reducing bacterium RBFL and iron-reducing bacterium FeRB-FL1404 volume ratio
: be configured to the bacterium solution of mixed bacterial at 2: 1.6.
Above-mentioned bacterium solution presses iron-reducing bacterium RBFL, iron-reducing bacterium FeRB-FL1404 and Geobacter bremensis
Straub and Buchholz-Cleven（ATCC BAA-607）Volume ratio be 3: 6: 2 bacterium solutions for being configured to mixed bacterial.
Above-mentioned bacterium solution presses iron-reducing bacterium FeRB-FL1404, iron-reducing bacterium FL-H1 and achromobacter（CCTCC AB
206571）Volume ratio be 5: 8: 2 bacterium solutions for being configured to mixed bacterial.
Above-mentioned bacterium solution is by iron-reducing bacterium RBFL3, the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）, achromobacter（CCTCC
AB 206571）With Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-607）Body
Bacterium solution of the product than being configured to mixed bacterial for 6: 1: 2: 3.
The application of described mixed bacterial, it is characterised in that the mixed bacterial is used to go back Fe in protokaolin2O3, by kaolinite
Insoluble ferric iron back is removed into soluble ferrous iron, then by separation of solid and liquid in soil, so as to reduce kaolin
Middle Fe2O3Content, improve kaolin whiteness.
Described application, it is characterised in that comprise the following steps：
1）It is 200-400 mesh materials that kaolin, which sieves to obtain particle diameter, adds water to be configured to certain density ore pulp A；
2）Carbon source, nitrogen source, surfactant and inorganic salts are added in above-mentioned ore pulp A, are configured to ore pulp B, described carbon source, nitrogen
The addition in source, surfactant and inorganic salts be respectively ore pulp A mass 1.0-2.5%, 1.0-2.5%, 0.005-0.01% and
0.005-0.01%；
3）The bacterium solution of any described mixed bacterial cultures of claim 1-7 is added in ore pulp B, addition is ore pulp A mass
10-15%, bacterial concentration 107 CFU/ml -109CFU/ml, prepare resulting mixture；
4）Mixture is carried out into iron removaling in 15-40 DEG C to react 8-15 days；
5）By reacted kaolin slurry through separation of solid and liquid, that is, obtain reducing Fe2O3Iron tramp content kaolin, warp
Its whiteness and Fe are determined after 105 DEG C of dryings2O3Content；
Step 2）Described in carbon source at least a kind of be selected from glucose, maltose, starch or molasses；At least a kind of choosing of nitrogen source
From peptone, yeast extract, analysis for soybean powder, ammonium nitrate, ammonium chloride or urea；Surfactant is at least a kind of to be selected from Tween 80, gathers
Ethylene glycol, dodecyl sodium sulfate or cetyl trimethylammonium bromide；Inorganic salts are at least a kind of to be selected from Na2HPO4、
NaH2PO4, sodium metaphosphate, MgSO4、CaCl2、ZnCl2Or CuCl2。
Beneficial effects of the present invention are as follows：
1st, Fe in kaolin is removed using mixed bacterial in the present invention2O3, in the kaolin mixed bacterial method for removing iron provided,
Kaolin ore slurry concentration is preferred, the selection of mixed bacterial and bacterial concentration are preferred, and mixed bacterial can be made to obtain kaolinic iron removaling
To giving full play to, increase Fe in kaolin2O3Removal effect.
2nd, beneficial effect of the present invention is：The mixed bacterial formed using this 7 kinds of bacterium various combinations is removed applied to kaolin
Iron is brightened with cost is low, energy consumption is small, environment-friendly etc., and the high iron low grade kaolin ore that script extraction value can be made not high obtains
With exploitation, extend mine life, realize comprehensive utilization of resources and sustainable development.
3rd, key point of the invention is that microbial strains Fe in protokaolin is gone back in mixed bacterial2O3During
Synergy, improve de-ironing efficiency.
Embodiment
It is used except iron bacteria is specific as follows in the embodiment of the present invention：
（1）Iron-reducing bacterium RBFL：Selected from Lin Yuman, Xu Xuping, Wei Zhaodi.Iron-reducing bacterium RBFL separation identification and its removal kaolinite
Fe in soil2O3.Fujian Normal University's journal, 2015,31（1）：73-78；
（2）Iron-reducing bacterium FL-H1：Selected from Lin Yuman, Chen Wenrui, Chen Zuliang.Iron grinds in iron-reducing bacterium FL-H1 removal kaolin
Study carefully.Nonmetallic ore, 2010,33（6）：11-14；
（3）Iron-reducing bacterium RBFL3：Selected from Wei Zhaodi, Xu Xuping, Chen Wenrui, Lin Yuman.Iron-reducing bacterium RBFL3 extracellular polymerics
Iron is studied also in protokaolin.Nonmetallic ore, 2015,38（1）：59-62；
（4）Iron-reducing bacterium FeRB-FL1404：Selected from Lin Yuman, Chen little Lan, Wei Zhaodi, Xu Xuping.One plant of iron-reducing bacterium FeRB-
Fe (III) is studied in FL1404 separation and identification and its removal kaolin.Chinese non-metallic mineral industry guide, 2015, (1)（Always
114th phase）：11-15；
（5）The hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol, from Chinese agriculture Microbiological Culture Collection administrative center（ACCC）, preserving number ACCC
00386；
（6）Geobacter bremensis Straub and Buchholz-Cleven, from ATCC BAA-607；
（7）Achromobacter, from CCTCC AB 206571.
Bacteria Culture is obtained into bacterium solution using this area conventional method culture.
The number of viable ratio of following 7 kinds of bacterium is 3：4-7：3-6：5-9：1：2：2-5：
1st, iron-reducing bacterium RBFL ((Bacillus pumilus, bacillus brevis)；
2nd, iron-reducing bacterium RBFL3 (Bacillus pumilus, bacillus pumilus)；
3rd, iron-reducing bacterium FeRB-FL1404 (Bacillus pumilus, bacillus pumilus)；
4th, iron-reducing bacterium FL-H1；
5th, the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）；
6th, achromobacter（CCTCC AB 206571）；
7th, Geobacter bremensis Straub and Buchholz-Cleven,（ATCC BAA-607）.
Kaolin used in the following embodiments of the present invention is provided by Longyan Gaolingtu Co., Ltd.
Embodiment 1：
1st, the preparation of mixed bacterial bacterium solution：
Iron-reducing bacterium RBFL, iron-reducing bacterium RBFL3 and iron-reducing bacterium FeRB-FL1404 it is activated respectively and expand culture obtain phase
Answer bacterium solution, viable bacteria concentration is 10 in respective bacterium solution7 CFU/ml -109CFU/ml；
The 2nd, the bacterium solution of above-mentioned preparation is pressed to iron-reducing bacterium RBFL, iron-reducing bacterium RBFL and iron-reducing bacterium FeRB-FL1404 volume
Than the bacterium solution that mixed bacterial is configured to for 1: 2: 1.6；
3rd, it is 200-400 mesh that kaolin, which is screened to particle diameter, adds water to prepare the kaolin ore slurry A of 10% mass fraction；
4th, the molasses of 1.2% ore pulp A mass, the peptone of 1.2% ore pulp A mass, 0.006% ore pulp A are added in above-mentioned ore pulp A
The MgSO of the Tween 80 of quality and 0.008% ore pulp A mass4,It is configured to ore pulp B；
5th, step step is added into ore pulp B（2）The bacterium solution of preparation, dosage are the 10% of ore pulp A mass；
6th, by step（5）Obtained mixture reacts 13 days at 25 DEG C carries out iron removaling；
7th, reacted kaolin is obtained removing Fe through separation of solid and liquid2O3Iron tramp kaolin.
8th, the kaolin after iron removaling determines its whiteness and Fe after 105 DEG C are dried2O3Content.
Embodiment 2
1st, the preparation of mixed bacterial bacterium solution：
Iron-reducing bacterium RBFL, iron-reducing bacterium FeRB-FL1404 and Geobacter bremensis Straub and
Buchholz-Cleven（ATCC BAA-607）It is activated respectively and expand culture and obtain corresponding bacterium solution, viable bacteria in respective bacterium solution
Concentration is 107 CFU/ml -109CFU/ml；
2nd, the bacterium solution of above-mentioned preparation is pressed into iron-reducing bacterium RBFL, iron-reducing bacterium FeRB-FL1404 and Geobacter bremensis
Straub and Buchholz-Cleven（ATCC BAA-607）Volume ratio be 3: 6: 2 bacterium solutions for being configured to mixed bacterial；
3rd, remaining operating procedure（3-8 steps）With embodiment l.
Embodiment 3
1st, the preparation of mixed bacterial bacterium solution：
Iron-reducing bacterium FeRB-FL1404, iron-reducing bacterium FL-H1 and achromobacter（CCTCC AB 206571）Respectively it is activated and
Expand culture and obtain corresponding bacterium solution, viable bacteria concentration is 10 in respective bacterium solution7 CFU/ml -109CFU/ml；
2nd, the bacterium solution of above-mentioned preparation is pressed into iron-reducing bacterium FeRB-FL1404, iron-reducing bacterium FL-H1 and achromobacter（CCTCC AB
206571）The volume ratio volume ratio that is be 5: 8: 2 bacterium solutions for being configured to mixed bacterial；
3rd, remaining operating procedure（3-8 steps）With embodiment l.
Embodiment 4
1st, the preparation of mixed bacterial bacterium solution：
The hot anaerobic bacillus(cillus anaerobicus) of iron-reducing bacterium RBFL3, producing and ethanol（ACCC 00386）, achromobacter（CCTCC AB 206571）With
Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-607）Activated respectively and expansion
Culture obtains corresponding bacterium solution, and viable bacteria concentration is 10 in respective bacterium solution7-109CFU/ml；
2nd, by the bacterium solution of above-mentioned preparation by iron-reducing bacterium RBFL3, the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）, achromobacter
（CCTCC AB 206571）With Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-
607）Volume ratio be 6: 1: 2: 3 bacterium solutions for being configured to mixed bacterial；
3rd, remaining operating procedure（3-8 steps）With embodiment l.
Embodiment 5
1st, the preparation of mixed bacterial bacterium solution：
The hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）And achromobacter（CCTCC AB 206571）Activated respectively and expansion training
Support and obtain corresponding bacterium solution, viable bacteria concentration is 10 in respective bacterium solution7-109CFU/ml；
2nd, the bacterium solution of above-mentioned preparation is pressed into the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC 00386）And achromobacter（CCTCC AB
206571）Volume ratio be 1：2 are configured to the bacterium solution of mixed bacterial；
3rd, remaining operating procedure is the same as embodiment l.
Above-mentioned five embodiment test results are shown in Table 1.
The blended bacterium of the kaolin of table 1 result before and after the processing
By can be seen that in above-described embodiment：The mixed bacterial formed using this 7 kinds of bacterium various combinations is applied to removing iron by kaolin
Brighten, make the Fe in kaolin2O3Content drops to 0.71%-0.39% by 0.93%, and it is big to improve kaolinic whiteness.It can make originally
The not high high iron low grade kaolin ore of extraction value is exploited, extend mine life, realize comprehensive utilization of resources and
Sustainable development.
Presently preferred embodiments of the present invention is the foregoing is only, all equivalent changes done according to the present patent application scope are with repairing
Decorations, it should all belong to the covering scope of the present invention.
Claims (9)
1. one kind goes back Fe in protokaolin2O3Mixed bacterial, it is characterised in that at least two kinds of described mixed bacterial is selected from
Iron-reducing bacterium RBFL ((Bacillus pumilus, bacillus brevis), iron-reducing bacterium RBFL3 (Bacillus pumilus,
Bacillus pumilus), iron-reducing bacterium FeRB-FL1404 (Bacillus pumilus, bacillus pumilus), iron-reducing bacterium FL-
The hot anaerobic bacillus(cillus anaerobicus) of H1, producing and ethanol（ACCC 00386）, achromobacter（CCTCC AB 206571）、Geobacter bremensis
Straub and Buchholz-Cleven（ATCC BAA-607）, each bacterium is activated respectively and expansion culture obtains corresponding bacterium
Liquid, viable bacteria concentration is 10 in respective bacterium solution7 CFU/ml -109CFU/ml, described iron-reducing bacterium RBFL ((Bacillus
Pumilus, bacillus brevis)：Iron-reducing bacterium RBFL3 (Bacillus pumilus, bacillus pumilus)：Iron-reducing bacterium
FeRB-FL1404 (Bacillus pumilus, bacillus pumilus)：Iron-reducing bacterium FL-H1：The hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol
（ACCC 00386）：Achromobacter（CCTCC AB 206571）：Geobacter bremensis Straub and
Buchholz-Cleven（ATCC BAA-607）Number of viable ratio be 3：4-7：3-6：5-9：1：2：2-5.
2. mixed bacterial as claimed in claim 1, it is characterised in that the concentration of bacterium is 10 in described mixed bacterial7
CFU/ml -109 CFU/ml。
3. mixed bacterial as claimed in claim 1 or 2, it is characterised in that bacterium solution presses the hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol（ACCC
00386）And achromobacter（CCTCC AB 206571）Volume ratio be 1：2 are configured to the bacterium solution of mixed bacterial.
4. mixed bacterial as claimed in claim 1 or 2, it is characterised in that bacterium solution presses iron-reducing bacterium RBFL, iron-reducing bacterium RBFL
Volume ratio with iron-reducing bacterium FeRB-FL1404 is 1: 2: 1.6 bacterium solution for being configured to mixed bacterial.
5. mixed bacterial as claimed in claim 1 or 2, it is characterised in that bacterium solution is by iron-reducing bacterium RBFL, iron-reducing bacterium
FeRB-FL1404 and Geobacter bremensis Straub and Buchholz-Cleven（ATCC BAA-607）Body
Bacterium solution of the product than being configured to mixed bacterial for 3: 6: 2.
6. mixed bacterial as claimed in claim 1 or 2, it is characterised in that bacterium solution presses iron-reducing bacterium FeRB-FL1404, iron also
Opportunistic pathogen FL-H1 and achromobacter（CCTCC AB 206571）Volume ratio be 5: 8: 2 bacterium solutions for being configured to mixed bacterial.
7. mixed bacterial as claimed in claim 1 or 2, it is characterised in that bacterium solution is detested by iron-reducing bacterium RBFL3, producing and ethanol heat
Oxygen bacillus（ACCC 00386）, achromobacter（CCTCC AB 206571）With Geobacter bremensis Straub and
Buchholz-Cleven（ATCC BAA-607）Volume ratio be 6: 1: 2: 3 bacterium solutions for being configured to mixed bacterial.
8. the application of any described mixed bacterials of claim 1-7, it is characterised in that the mixed bacterial is used to go back protokaolin
Middle Fe2O3, the ferrous iron by ferric iron back insoluble in kaolin into solubility, then removed by separation of solid and liquid, so as to
Reduce Fe in kaolin2O3Content, improve kaolin whiteness.
9. application as claimed in claim 8, it is characterised in that comprise the following steps：
1）It is 200-400 mesh materials that kaolin, which sieves to obtain particle diameter, adds water to be configured to certain density ore pulp A；
2）Carbon source, nitrogen source, surfactant and inorganic salts are added in above-mentioned ore pulp A, are configured to ore pulp B, described carbon source, nitrogen
The addition in source, surfactant and inorganic salts be respectively ore pulp A mass 1.0-2.5%, 1.0-2.5%, 0.005-0.01% and
0.005-0.01%；
3）The bacterium solution of any described mixed bacterial cultures of claim 1-7 is added in ore pulp B, addition is ore pulp A mass
10-15%, bacterial concentration 107 CFU/ml -109CFU/ml, prepare resulting mixture；
4）Mixture is carried out into iron removaling in 15-40 DEG C to react 8-15 days；
5）By reacted kaolin slurry through separation of solid and liquid, that is, obtain reducing Fe2O3Iron tramp content kaolin, warp
Its whiteness and Fe are determined after 105 DEG C of dryings2O3Content；
Step 2）Described in carbon source at least a kind of be selected from glucose, maltose, starch or molasses；At least a kind of choosing of nitrogen source
From peptone, yeast extract, analysis for soybean powder, ammonium nitrate, ammonium chloride or urea；Surfactant is at least a kind of to be selected from Tween 80, gathers
Ethylene glycol, dodecyl sodium sulfate or cetyl trimethylammonium bromide；Inorganic salts are at least a kind of to be selected from Na2HPO4、
NaH2PO4, sodium metaphosphate, MgSO4、CaCl2、ZnCl2Or CuCl2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710997647.XA CN107603913A (en) | 2017-10-24 | 2017-10-24 | A kind of mixed bacterial and its application in removing iron by kaolin brightens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710997647.XA CN107603913A (en) | 2017-10-24 | 2017-10-24 | A kind of mixed bacterial and its application in removing iron by kaolin brightens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107603913A true CN107603913A (en) | 2018-01-19 |
Family
ID=61079476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710997647.XA Pending CN107603913A (en) | 2017-10-24 | 2017-10-24 | A kind of mixed bacterial and its application in removing iron by kaolin brightens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107603913A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109809423A (en) * | 2019-03-11 | 2019-05-28 | 福建师范大学 | A kind of method that composite nutrient removes iron for kaolin microorganism |
| CN109809413A (en) * | 2019-03-11 | 2019-05-28 | 福建师范大学 | A kind of quartz sand microorganism iron-removal and whitening method |
| CN109809424A (en) * | 2019-03-11 | 2019-05-28 | 福建师范大学 | A kind of low cost culture Mixed Microbes are to the method for removing iron by kaolin |
| CN109971792A (en) * | 2019-03-11 | 2019-07-05 | 福建师范大学 | A kind of kaolin microorganism method for removing iron using chrysanthemum slag extract as nutrients |
| WO2021068416A1 (en) * | 2019-10-12 | 2021-04-15 | 湖南恒凯环保科技投资有限公司 | Brevibacillus nitrificans strain yj1 and application thereof |
| CN116285928A (en) * | 2023-04-24 | 2023-06-23 | 长江大学 | Method for in-situ removal of reservoir blockage and reservoir oil displacement by utilizing microorganisms and iron reducing bacteria activator |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101490242A (en) * | 2006-05-22 | 2009-07-22 | 比奥咖索尔Ipr有限公司 | Thermoanaerobacter mathranii strain BGl |
-
2017
- 2017-10-24 CN CN201710997647.XA patent/CN107603913A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101490242A (en) * | 2006-05-22 | 2009-07-22 | 比奥咖索尔Ipr有限公司 | Thermoanaerobacter mathranii strain BGl |
Non-Patent Citations (7)
| Title |
|---|
| IVETA STYRIAKOVÁ等: "NUTRIENTS ENHANCING THE BACTERIAL IRON DISSOLUTION IN THE PROCESSING OF FELDSPAR RAW MATERIALS", 《CERAMICS-SILIKÁTY》 * |
| KRISTINA L. STRAUB等: "Geobacter bremensis sp. nov. and Geobacter pelophilus sp. nov., two dissimilatory ferric-iron-reducing bacteria", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》 * |
| 林玉满 等: "一株铁还原菌FeRB-FL1404的分离和鉴定及其去除高岭土中Fe(Ⅲ)研究", 《中国非金属矿工业导刊》 * |
| 林玉满 等: "铁还原菌 RBFL 分离鉴定及其去除高岭土中 Fe2O3", 《福建师范大学学报》 * |
| 林玉满 等: "铁还原菌FL-H1去除高岭土中铁的研究", 《非金属矿》 * |
| 王文铜: "花生根际土壤细菌群落结构分析", 《中国优秀硕士论文全文数据库农业科技辑》 * |
| 魏招娣 等: "铁还原菌RBFL3胞外聚合物还原高岭土中铁研究", 《非金属矿》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109809423A (en) * | 2019-03-11 | 2019-05-28 | 福建师范大学 | A kind of method that composite nutrient removes iron for kaolin microorganism |
| CN109809413A (en) * | 2019-03-11 | 2019-05-28 | 福建师范大学 | A kind of quartz sand microorganism iron-removal and whitening method |
| CN109809424A (en) * | 2019-03-11 | 2019-05-28 | 福建师范大学 | A kind of low cost culture Mixed Microbes are to the method for removing iron by kaolin |
| CN109971792A (en) * | 2019-03-11 | 2019-07-05 | 福建师范大学 | A kind of kaolin microorganism method for removing iron using chrysanthemum slag extract as nutrients |
| WO2021068416A1 (en) * | 2019-10-12 | 2021-04-15 | 湖南恒凯环保科技投资有限公司 | Brevibacillus nitrificans strain yj1 and application thereof |
| CN116285928A (en) * | 2023-04-24 | 2023-06-23 | 长江大学 | Method for in-situ removal of reservoir blockage and reservoir oil displacement by utilizing microorganisms and iron reducing bacteria activator |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107603913A (en) | A kind of mixed bacterial and its application in removing iron by kaolin brightens | |
| CN107601519A (en) | A kind of low-grade kaolin bacterium iron-removal and whitening method | |
| CN107176702A (en) | A kind of sewage water treatment method for strengthening sulphur autotrophic denitrification process synchronous denitrification dephosphorizing | |
| Guo et al. | Bioleaching of iron from kaolin using Fe (III)-reducing bacteria with various carbon nitrogen sources | |
| CN103031434B (en) | Refractory gold ore desulfurizing and dearsenifying method | |
| CN103014336B (en) | Preparation and method for treating high-arsenic and high-sulfur gold ore | |
| CN106190871B (en) | Method for treating heavy metal contaminated soil by bioleaching with composite filamentous fungi by taking straws as carbon source | |
| CN102745683B (en) | Biological oxidation graphite and preparation method thereof | |
| Wu et al. | Isolation of an acid producing Bacillus sp. EEEL02: Potential for bauxite residue neutralization | |
| CN103013847A (en) | Ammonia-producing mineral leaching bacterium JAT-1 as well as culture method and application of ammonia-producing mineral leaching bacterium JAT-1 | |
| Hemati et al. | In vitro evaluation of lignocellulolytic activity of thermophilic bacteria isolated from different composts and soils of Iran | |
| CN105923921B (en) | The treatment process of gourmet powder fermenting waste water | |
| CN103088222B (en) | Process for treating refractory gold ores | |
| CN103555620A (en) | Alkaline catalase high-yielding strain and fermentation-method production process thereof | |
| CN113201648A (en) | Microbial leaching method for iron tailings cobalt element | |
| CN103361288A (en) | Methylotrophic bacillus and application thereof | |
| CN103320610B (en) | Sorting technique for valuable elements associated with gold | |
| CN109809413A (en) | A kind of quartz sand microorganism iron-removal and whitening method | |
| CN109554303B (en) | Microbacterium aurantiacus and preparation and application thereof | |
| CN109554541A (en) | A kind of method of mica in removing magnetic separation of iron ore concentrate | |
| CN114644985B (en) | Filamentous fungi and method for recycling phosphorus in sludge by using same | |
| CN103205381B (en) | Decarbonizing and desulphurizing bacterium agent for difficultly-selected gold ore and application thereof | |
| CN105502318B (en) | A kind of method that utilization phosphorus ore pit water microorganism species dissolve mid low grade phosphate rock | |
| CN105752955B (en) | A kind of method that mid low grade phosphate rock is dissolved using active sludge microorganism flora | |
| CN103205571B (en) | Treatment process for high-carbon high-arsenic gold ores |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180119 |
|
| WD01 | Invention patent application deemed withdrawn after publication |