CN109809424A - A kind of low cost culture Mixed Microbes are to the method for removing iron by kaolin - Google Patents

Abstract

The invention belongs to kaolin processing technique field, in particular to method of a kind of low cost culture Mixed Microbes to removing iron by kaolin；Chinese medicine slag cooperation banana stem leaves, agent of lactic acid bacteria, rice bran are specifically discarded as culture medium primary raw material by selecting, specific combination strain is cultivated, combination strain bacterium solution is made, is cultivated using bacterium in combination strain bacterium solution to logarithmic phase, for removing iron by kaolin, by Fe insoluble in kaolin₂O₃It is reduced into soluble Fe²⁺；Experiment shows that the bacterium for the culture medium culture that the present invention uses reacts with kaolin, removes Fe in kaolin₂O₃, Fe₂O₃Content reduces obviously, to significantly improve kaolin whiteness, 0.2g/L NTA is added in the present invention in iron-reducing bacterium and kaolin hybrid reaction system, and bacterial reduction kaolin Fe can be improved as complex compound in NTA₂O₃Amount, to further increase kaolinic whiteness obtained.

Description

A kind of low cost culture Mixed Microbes are to the method for removing iron by kaolin

Technical field

The invention belongs to kaolin processing technique field, in particular to a kind of low cost culture Mixed Microbes are to removing iron by kaolin Method.

Background technique

Kaolin is a kind of nonmetallic minerals with excellent processing performance and significant application value, mainly by kaolinite, Mica, feldspar and quartz composition.Kaolin purposes is very extensive, for papermaking, ceramics, refractory material, coating, gum filler, Frit for porcelain enamel and white cement raw material etc..In recent years, market is being continuously increased kaolinic demand, special papermaking and ceramic row It is more demanding to kaolinic whiteness in industry.Iron tramp is one of kaolin coloring components, influences kaolin whiteness and application.Cause This, reduces kaolin coloring matter Fe₂O₃Content, so that it may achieve the purpose that improve kaolin whiteness.

Kaolinic bleaching generallys use physical method and chemical method at present.Physical method is such as: (1) wet cleaning process: removing Also part iron mineral is removed while the impurity such as quartz, mica, and whiteness is made to increase；(2) magnetic method: main remove has one Determine the oxide of Armco magnetic iron, removal effect is related with impurity Armco magnetic iron power.Chemical method mainly has: (1) acid-hatching of young eggs；(2) it restores Method, such as by Fe insoluble in kaolin₂O₃It is reduced into soluble Fe²⁺, then by being separated by solid-liquid separation removal；(3) oxidizing process (4) oxygen Change-reduction method.Physical method and chemical method iron-removal and whitening are big in the prevalence for the treatment of process energy consumption, operating cost is high, may be used also The disadvantages of capable of causing environmental pollution.

Mineral biology (mainly bacterium) processing technology is an emerging mineral processing technology, and distinguishing feature is investment Less, at low cost, energy consumption is small, environmental-friendly, can overcome the shortcomings of physical method and chemical method mineral process iron removal technology.Bacterium removes Iron is will be by Fe insoluble in kaolin based on bacterium₂O₃Reduction is converted to soluble Fe²⁺, then by being separated by solid-liquid separation removal, drop Fe in low kaolin₂O₃Content improves kaolinic whiteness.

Summary of the invention

The technical problems to be solved by the present invention are: providing a kind of low cost culture Mixed Microbes to the side of removing iron by kaolin Method.

In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows:

A kind of low cost culture Mixed Microbes are to the method for removing iron by kaolin, comprising the following steps:

Step 1: banana stem leaves, 10-20 parts of the ground speiss, 10-20 parts of rhizoma atractylodis slags, 5-10 parts of days that 130-150 parts are dried The dregs of linseeds or sesame seeds and 8-12 parts of rice bran mixing, crush, 10-15 times of quality clear water are added, in 80 DEG C of heat preservation 3-4h, filtering obtains filtrate A；

Step 2: pH value will be adjusted after filtrate A cooling between 6-6.5,4-6 parts of agent of lactic acid bacteria, bacillus subtilises are added 20 parts of preparation, 10 parts of amylase obtain solution B in 40-60 DEG C, heat preservation 60-100min；

Step 3: the pH of solution B being adjusted to 4.0-5.5,10 parts of carbohydrase are added, in 40-60 DEG C of standing 20-24h, is obtained To solution C；

Step 4: 6-9 parts of calcium phosphate, 1-3 parts of urea, 0.03-0.05 parts of sodium phosphates, 0.10-2.0 parts being added in solution C Dodecyl sodium sulfate adjusts pH to 6.5-7.5, obtains solution D；

Step 5: by solution D after 121 DEG C of sterilizings, after being cooled to 20-25 DEG C, mixed bacteria liquid is added.The mixed bacteria liquid It is pressed by Klebsiella oxytoca IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 It is mixed to get according to number of viable than 3: 6: 7,30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁷-10⁹CFU/mL is obtained To mixed bacteria liquid；

Step 6: being that add tap water to be configured to mass fraction be 10-25% ore pulp to 200-400 mesh kaolin by partial size, be added Above-mentioned mixed bacteria liquid, solution C and 0.2g/L NTA, 25-35 DEG C of insulation reaction 8-15d, insoluble Fe in kaolin₂O₃Also by bacterium Original is at soluble Fe²⁺.The volume ratio of the NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 20: 1- 3, the volume ratio of the kaolin ore slurry and solution C is 10: 1-2；

Step 7: by the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid is through overdrying The kaolin of iron-removal and whitening is arrived after dry.

The beneficial effects of the present invention are: a kind of low cost culture Mixed Microbes provided by the invention are to the side of removing iron by kaolin In method, by selecting, specifically discarded Chinese medicine slag cooperation banana stem leaves, agent of lactic acid bacteria, rice bran are trained as culture medium primary raw material Specific combination strain is supported, combination strain bacterium solution is made, is cultivated using bacterium in combination strain bacterium solution to logarithmic phase, is used for removing iron by kaolin, By Fe insoluble in kaolin₂O₃It is reduced into soluble Fe²⁺；Experiment show the bacterium of the culture medium culture that the present invention uses with Kaolin reaction, removes Fe in kaolin₂O₃, Fe₂O₃Content reduces obviously, so that kaolin whiteness is significantly improved, the present invention In, the combination of banana stem leaves, glutinous rehmannia, rhizoma atractylodis, Rhizoma Gastrodiae, rice bran provides carbon source and nitrogen source not just for culture medium, in Chinese medicine slag Contain or generate the substance for promoting the reaction of iron-reducing bacterium reduced iron by reaction, such as is rich in oxalate in culture medium of the invention Ion, oxalate denominationby can have apparent facilitation to micro-reduction iron, and the present invention is mixed in iron-reducing bacterium and kaolin It closes and 0.2g/L NTA is added in reaction system, bacterial reduction kaolin Fe can be improved as complex compound in NTA₂O₃Amount, thus Further increase kaolinic whiteness obtained.

Specific embodiment

To explain the technical content, the achieved purpose and the effect of the present invention in detail, it is explained below in conjunction with embodiment.

The most critical design of the present invention is: by select specifically discarded Chinese medicine slag cooperate banana stem leaves, agent of lactic acid bacteria, Rice bran, as culture medium primary raw material, cultivate specific combination strain, be made combination strain bacterium solution, be used for removing iron by kaolin, by kaolinite Insoluble Fe in soil₂O₃It is reduced into soluble Fe²⁺。

The present invention provides a kind of low cost culture Mixed Microbes to the method for removing iron by kaolin, comprising the following steps:

Step 1: banana stem leaves, 10-20 parts of the ground speiss, 10-20 parts of rhizoma atractylodis slags, 5-10 parts of days that 130-150 parts are dried The dregs of linseeds or sesame seeds and 8-12 parts of rice bran mixing, crush, 10-15 times of quality clear water are added, in 80 DEG C of heat preservation 3-4h, filtering obtains filtrate A；

Step 2: pH value will be adjusted after filtrate A cooling between 6-6.5,4-6 parts of agent of lactic acid bacteria, bacillus subtilises are added 20 parts of preparation, 10 parts of amylase obtain solution B in 40-60 DEG C, heat preservation 60-100min；

Step 3: the pH of solution B being adjusted to 4.0-5.5,10 parts of carbohydrase are added, in 40-60 DEG C of standing 20-24h, is obtained To solution C；

Step 4: 6-9 parts of calcium phosphate, 1-3 parts of urea, 0.03-0.05 parts of sodium phosphates, 0.10-2.0 parts being added in solution C Dodecyl sodium sulfate adjusts pH to 6.5-7.5, obtains solution D；

Step 5: by solution D after 121 DEG C of sterilizings, after being cooled to 20-25 DEG C, mixed bacteria liquid is added.The mixed bacteria liquid It is pressed by Klebsiella oxytoca IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 It is mixed to get according to number of viable than 3: 6: 7,30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁷-10⁹CFU/mL is obtained To mixed bacteria liquid；

Step 6: being that add tap water to be configured to mass fraction be 10-25% ore pulp to 200-400 mesh kaolin by partial size, be added Above-mentioned mixed bacteria liquid, solution C and 0.2g/L NTA, 25-35 DEG C of insulation reaction 8-15d, insoluble Fe in kaolin₂O₃Also by bacterium Original is at soluble Fe²⁺.The volume ratio of the NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 20: 1- 3, the volume ratio of the kaolin ore slurry and solution C is 10: 1-2；

Step 7: by the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid is through overdrying The kaolin of iron-removal and whitening is arrived after dry.

A kind of above-mentioned low cost culture Mixed Microbes remark additionally to the strain in the method for removing iron by kaolin:

Klebsiella oxytoca, IMFRCUG-1 are preserved in China typical culture collection center on May 27th, 2015, It is referred to as CCTCC, deposit number M2015332；

Iron-reducing bacterium FeRB-FL1401 is selected from Lin Yuman, Chen little Lan, Wei Zhaodi, Xu Xuping.One plant of iron-reducing bacterium Fe (III) research in the separation and identification of FeRBFL1404 and its removal kaolin.Chinese non-metallic mineral industry guide, 2015, (1) (total 114th phase): 11-15；

Iron-reducing bacterium RBEL3: Wei Zhaodi, Xu Xuping, Chen Wenrui, Lin Yuman are selected from.Iron-reducing bacterium RBEL3 extracellular polymeric The research of iron also in protokaolin.Nonmetallic ore, 2015,38 (1): 59-62；

A kind of above-mentioned low cost culture Mixed Microbes specifically discard Chinese medicine slag by selecting in the method for removing iron by kaolin Cooperate banana stem leaves, agent of lactic acid bacteria, rice bran as culture medium primary raw material, cultivate specific combination strain, combination strain bacterium solution is made, It is cultivated using bacterium in combination strain bacterium solution to logarithmic phase, removing iron by kaolin is used for, by Fe insoluble in kaolin₂O₃It is reduced into solvable Fe²⁺；Experiment shows that the bacterium for the culture medium culture that the present invention uses reacts with kaolin, removes Fe in kaolin₂O₃, Fe₂O₃Content reduces obviously, so that kaolin whiteness is significantly improved, in the present invention, banana stem leaves, glutinous rehmannia, rhizoma atractylodis, Rhizoma Gastrodiae, rice The combination of chaff provides carbon source and nitrogen source not just for culture medium, contains in Chinese medicine slag or generates promotion iron-reducing bacterium by reaction The substance of reduced iron reaction, such as oxalate denominationby is rich in culture medium of the invention, oxalate denominationby can be to microorganism also Former iron has apparent facilitation, and 0.2g/L NTA, NTA is added in the present invention in iron-reducing bacterium and kaolin hybrid reaction system As complex compound, bacterial reduction kaolin Fe can be improved₂O₃Amount, to further increase kaolinic whiteness obtained.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 1 specifically:

Banana stem leaves, 15 parts of ground speiss, 15 parts of rhizoma atractylodis slags, 7 parts of Rhizoma Gastrodiae residues and the 10 parts of rice bran mixing that 140 parts are dried, It crushes, 12 times of quality clear water is added, in 80 DEG C of heat preservation 3.5h, filtering obtains filtrate A.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 2 specifically:

Will filtrate A it is cooling after adjust pH value between 6.2, be added 5 parts of agent of lactic acid bacteria, 20 parts of bacillus subtilis formulation, 10 parts of amylase, in 50 DEG C, heat preservation 80min, obtain solution B.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 3 specifically:

The pH of solution B is adjusted to 4.7,10 parts of carbohydrase are added, in 50 DEG C of standing 22h, obtains solution C.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 4 specifically:

7.5 parts of calcium phosphate, 2 parts of urea, 0.04 part of sodium phosphate, 1.0 parts of dodecyl sodium sulfates are added in solution C, adjust PH to 7.0, obtains solution D.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 5 specifically:

By solution D after 121 DEG C of sterilizings, after being cooled to 22 DEG C, mixed bacteria liquid is added.The mixed bacteria liquid is by producing sour Cray Primary bacterium IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 are according to number of viable It is mixed to get than 3: 6: 7,30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁸CFU/mL obtains mixed bacteria liquid.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 6 specifically:

Be that add tap water to be configured to mass fraction be 17% ore pulp to 300 mesh kaolin by partial size, be added above-mentioned mixed bacteria liquid, Solution C and 0.2g/L NTA, 30 DEG C of insulation reaction 11d, insoluble Fe in kaolin₂O₃By bacterial reduction at soluble Fe²⁺.It is described The volume ratio of NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 10: 1, the kaolin ore slurry with it is molten The volume ratio of liquid C is 20: 3.

Further, above-mentioned low cost culture Mixed Microbes are in the method for removing iron by kaolin, the step 7 specifically:

By the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid passes through 105 DEG C of dryings The kaolin of iron-removal and whitening is arrived after 15min.

Embodiment 1

The present invention provides a kind of low cost culture Mixed Microbes to the method for removing iron by kaolin, comprising the following steps:

Step 1: banana stem leaves, 15 parts of ground speiss, 15 parts of rhizoma atractylodis slags, 7 parts of Rhizoma Gastrodiae residues and the 10 parts of rice brans that 140 parts are dried Mixing crushes, 12 times of quality clear water is added, and in 80 DEG C of heat preservation 3.5h, filtering obtains filtrate A；

Step 2: will filtrate A it is cooling after adjust pH value between 6.2,5 parts of agent of lactic acid bacteria, bacillus subtilis formulations are added 20 parts, 10 parts of amylase obtain solution B in 50 DEG C, heat preservation 80min；

Step 3: the pH of solution B being adjusted to 4.7,10 parts of carbohydrase are added, in 50 DEG C of standing 22h, obtains solution C；

Step 4: 7.5 parts of calcium phosphate, 2 parts of urea, 0.04 part of sodium phosphate, 1.0 parts of dodecyl sodium sulfonates being added in solution C Sodium adjusts pH to 7.0, obtains solution D；

Step 5: by solution D after 121 DEG C of sterilizings, after being cooled to 22 DEG C, mixed bacteria liquid is added.The mixed bacteria liquid is by producing Sour klebsiella IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 are according to work Bacterium number amount is mixed to get than 3: 6: 7, and 30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁸CFU/mL is mixed Bacterium solution；

Step 6: being that add tap water to be configured to mass fraction be 17% ore pulp to 300 mesh kaolin by partial size, be added above-mentioned mixed Combined bacteria liquid, solution C and 0.2g/L NTA, 30 DEG C of insulation reaction 11d, insoluble Fe in kaolin₂O₃By bacterial reduction at soluble Fe²⁺.The volume ratio of the NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 10: 1, the kaolin The volume ratio of ore pulp and solution C is 20: 3；

Step 7: by the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid passes through 105 To get the kaolin for arriving iron-removal and whitening after DEG C dry 15min.

Embodiment 2

The present invention provides a kind of low cost culture Mixed Microbes to the method for removing iron by kaolin, comprising the following steps:

Step 1: banana stem leaves, 10 parts of ground speiss, 10 parts of rhizoma atractylodis slags, 5 parts of Rhizoma Gastrodiae residues and the 8 parts of rice brans that 130 parts are dried Mixing crushes, 10 times of quality clear water is added, and in 80 DEG C of heat preservation 3h, filtering obtains filtrate A；

Step 2: will filtrate A it is cooling after adjust pH value between 6,4 parts of agent of lactic acid bacteria, bacillus subtilis formulation 20 are added Part, 10 parts of amylase obtain solution B in 40 DEG C, heat preservation 60min；

Step 3: the pH of solution B being adjusted to 4.0,10 parts of carbohydrase are added, in 40 DEG C of standing 20h, obtains solution C；

Step 4: 6 parts of calcium phosphate, 1 part of urea, 0.03 part of sodium phosphate, 0.10 part of dodecyl sodium sulfonate being added in solution C Sodium adjusts pH to 6.5, obtains solution D；

Step 5: by solution D after 121 DEG C of sterilizings, after being cooled to 20 DEG C, mixed bacteria liquid is added.The mixed bacteria liquid is by producing Sour klebsiella IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 are according to work Bacterium number amount is mixed to get than 3: 6: 7, and 30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁷CFU/mL is mixed Bacterium solution；

Step 6: being that add tap water to be configured to mass fraction be 10% ore pulp to 200 mesh kaolin by partial size, be added above-mentioned mixed Combined bacteria liquid, solution C and 0.2g/L NTA, 25 DEG C of insulation reaction 8d, insoluble Fe in kaolin₂O₃By bacterial reduction at soluble Fe^{2 +}.The volume ratio of the NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 20: 1, the kaolin ore The volume ratio of slurry and solution C is 10: 1；

Step 7: by the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid passes through 105 To get the kaolin for arriving iron-removal and whitening after DEG C dry 15min.

Embodiment 3

The present invention provides a kind of low cost culture Mixed Microbes to the method for removing iron by kaolin, comprising the following steps:

Step 1: banana stem leaves, 20 parts of ground speiss, 20 parts of rhizoma atractylodis slags, 10 parts of Rhizoma Gastrodiae residues and the 12 portions of rice that 150 parts are dried Chaff mixing, crushes, 15 times of quality clear water is added, and in 80 DEG C of heat preservation 4h, filtering obtains filtrate A；

Step 2: will filtrate A it is cooling after adjust pH value between 6.5,6 parts of agent of lactic acid bacteria, bacillus subtilis formulations are added 20 parts, 10 parts of amylase obtain solution B in 60 DEG C, heat preservation 100min；

Step 3: the pH of solution B being adjusted to 5.5,10 parts of carbohydrase are added, is stood for 24 hours in 60 DEG C, obtains solution C；

Step 4: 9 parts of calcium phosphate, 3 parts of urea, 0.05 part of sodium phosphate, 2.0 parts of dodecyl sodium sulfonates being added in solution C Sodium adjusts pH to 7.5, obtains solution D；

Step 5: by solution D after 121 DEG C of sterilizings, after being cooled to 25 DEG C, mixed bacteria liquid is added.The mixed bacteria liquid is by producing Sour klebsiella IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 are according to work Bacterium number amount is mixed to get than 3: 6: 7, and 30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁹CFU/mL is mixed Bacterium solution；

Step 6: being that add tap water to be configured to mass fraction be 25% ore pulp to 400 mesh kaolin by partial size, be added above-mentioned mixed Combined bacteria liquid, solution C and 0.2g/L NTA, 35 DEG C of insulation reaction 15d, insoluble Fe in kaolin₂O₃By bacterial reduction at soluble Fe²⁺.The volume ratio of the NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 20: 1-3, the kaolinite The volume ratio of native ore pulp and solution C is 5: 1；

Step 7: by the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid passes through 105 To get the kaolin for arriving iron-removal and whitening after DEG C dry 15min.

Kaolin of the low cost culture Mixed Microbes that above-mentioned 3 embodiments are related to the method for removing iron by kaolin before and after the processing Whiteness detected, as a result as shown in the table:

As seen from the above table, height made from a kind of method of the low cost culture Mixed Microbes of the present invention to removing iron by kaolin Ridge soil compares untreated kaolin, Fe₂O₃Content is substantially reduced, natural whiteness and 1280 DEG C of kaolin sintered into Whiteness have and significantly promoted.

In conclusion a kind of low cost culture Mixed Microbes provided by the invention pass through choosing in the method for removing iron by kaolin It uses and specifically discards Chinese medicine slag cooperation banana stem leaves, agent of lactic acid bacteria, rice bran as culture medium primary raw material, cultivate specific combination Bacterium, be made combination strain bacterium solution, cultivated using bacterium in combination strain bacterium solution to logarithmic phase, be used for removing iron by kaolin, by kaolin not Molten Fe₂O₃It is reduced into soluble Fe²⁺；Experiment shows that the bacterium for the culture medium culture that the present invention uses reacts with kaolin, goes Except Fe in kaolin₂O₃, Fe₂O₃Content reduces obviously, so that kaolin whiteness is significantly improved, in the present invention, banana stem leaves, The combination of glutinous rehmannia, rhizoma atractylodis, Rhizoma Gastrodiae, rice bran provides carbon source and nitrogen source not just for culture medium, contains in Chinese medicine slag or by anti- The substance for promoting the reaction of iron-reducing bacterium reduced iron should be generated, such as is rich in oxalate denominationby, oxalate in culture medium of the invention Ion can have apparent facilitation to micro-reduction iron, and the present invention is in iron-reducing bacterium and kaolin hybrid reaction system 0.2g/L NTA is added, bacterial reduction kaolin Fe can be improved as complex compound in NTA₂O₃Amount, to further increase system The kaolinic whiteness obtained.

The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair Equivalents made by bright description are applied directly or indirectly in relevant technical field, are similarly included in this hair In bright scope of patent protection.

Claims (8)

1. a kind of low cost culture Mixed Microbes are to the method for removing iron by kaolin, which comprises the following steps:

Step 1: banana stem leaves, 10-20 parts of the ground speiss, 10-20 parts of rhizoma atractylodis slags, 5-10 parts of Rhizoma Gastrodiae residues that 130-150 parts are dried It mixes, crushes with 8-12 parts of rice brans, 10-15 times of quality clear water is added, in 80 DEG C of heat preservation 3-4h, filtering obtains filtrate A；

Step 2: pH value will be adjusted after filtrate A cooling between 6-6.5,4-6 parts of agent of lactic acid bacteria, bacillus subtilis formulations are added 20 parts, 10 parts of amylase obtain solution B in 40-60 DEG C, heat preservation 60-100min；

Step 3: the pH of solution B being adjusted to 4.0-5.5,10 parts of carbohydrase are added, in 40-60 DEG C of standing 20-24h, is obtained molten Liquid C；

Step 4: 6-9 parts of calcium phosphate, 1-3 parts of urea, 0.03-0.05 parts of sodium phosphates, 0.10-2.0 part 12 being added in solution C Sodium alkyl sulfonate adjusts pH to 6.5-7.5, obtains solution D；

Step 5: by solution D after 121 DEG C of sterilizings, after being cooled to 20-25 DEG C, mixed bacteria liquid is added, the mixed bacteria liquid is by producing Sour klebsiella IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 are according to work Bacterium number amount is mixed to get than 3: 6: 7, and 30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁷-10⁹CFU/mL is mixed Combined bacteria liquid；

Step 6: being that add tap water to be configured to mass fraction be 10-25% ore pulp to 200-400 mesh kaolin by partial size, be added above-mentioned Mixed bacteria liquid, solution C and 0.2g/L NTA, 25-35 DEG C of insulation reaction 8-15d, insoluble Fe in kaolin₂O₃By bacterial reduction at Soluble Fe²⁺, the volume ratio of the NTA and bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 20: 1-3, institute The volume ratio for stating kaolin ore slurry and solution C is 10: 1-2；

Step 7: will by the obtained kaolin ore slurry of step 6 reaction through being separated by solid-liquid separation, isolated solid after drying, Obtain the kaolin of iron-removal and whitening.

2. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 1 specifically:

Banana stem leaves, 15 parts of ground speiss, 15 parts of rhizoma atractylodis slags, 7 parts of Rhizoma Gastrodiae residues and the 10 parts of rice bran mixing that 140 parts are dried, crush, 12 times of quality clear water are added, in 80 DEG C of heat preservation 3.5h, filtering obtains filtrate A.

3. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 2 specifically:

Will filtrate A it is cooling after adjust pH value between 6.2,5 parts of agent of lactic acid bacteria, 20 parts of bacillus subtilis formulation, starch are added 10 parts of enzyme, in 50 DEG C, heat preservation 80min, obtain solution B.

4. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 3 specifically:

The pH of solution B is adjusted to 4.7,10 parts of carbohydrase are added, in 50 DEG C of standing 22h, obtains solution C.

5. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 4 specifically:

7.5 parts of calcium phosphate, 2 parts of urea, 0.04 part of sodium phosphate, 1.0 parts of dodecyl sodium sulfates are added in solution C, adjust pH extremely 7.0, obtain solution D.

6. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 5 specifically:

By solution D after 121 DEG C of sterilizings, after being cooled to 22 DEG C, mixed bacteria liquid is added, the mixed bacteria liquid is by Klebsiella oxytoca IMFRCUG-1 (CCTCC M2015332), iron-reducing bacterium RBEL3 and iron-reducing bacterium FeRB-FL1401 are according to number of viable than 3: 6 : 7 are mixed to get, and 30 DEG C of cultures of the Mixed Microbes make bacterial concentration 10 in bacterium solution⁸CFU/mL obtains mixed bacteria liquid.

7. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 6 specifically:

It is that 300 mesh kaolin add tap water to be configured to mass fraction to be 17% ore pulp, above-mentioned mixed bacteria liquid, solution is added by partial size C and 0.2g/L NTA, 30 DEG C of insulation reaction 11d, insoluble Fe in kaolin₂O₃By bacterial reduction at soluble Fe²⁺, the NTA Volume ratio with bacterium solution is 1: 20, and the volume ratio of the kaolin ore slurry and bacterium solution is 10: 1, the kaolin ore slurry and solution C Volume ratio be 20: 3.

8. low cost culture Mixed Microbes according to claim 1 are to the method for removing iron by kaolin, which is characterized in that the step Rapid 7 specifically:

By the kaolin ore slurry obtained by step 6 reaction through being separated by solid-liquid separation, isolated solid passes through 105 DEG C of dryings The kaolin of iron-removal and whitening is arrived after 15min.