CN107582571A - A kind of medicine for treating tinea pedis and preparation method thereof - Google Patents
A kind of medicine for treating tinea pedis and preparation method thereof Download PDFInfo
- Publication number
- CN107582571A CN107582571A CN201710946037.7A CN201710946037A CN107582571A CN 107582571 A CN107582571 A CN 107582571A CN 201710946037 A CN201710946037 A CN 201710946037A CN 107582571 A CN107582571 A CN 107582571A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- tinea pedis
- extract
- temperature
- american
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of process for preparing medicine for treating tinea pedis.A, American cockroach crushes, and ethanol soaks to obtain mixed liquor；B, 30~50 DEG C of ultrasonic extractions of mixed liquor, the 30KHz of ultrasonic power 20, obtain ethanol extract；C, ethanol extract is concentrated to give American cockroach polypide ethanol concentrate；D, ethanol concentrate adds 7~10 times of water, and stirring, heating, standing are allowed to be layered；E, lower floor's aqueous, filtering, charcoal absorption obtained by step D；F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, washing active carbon, and water lotion discards；Eluent, filter and must elute filtrate；Elution filtrate obtained by step F is concentrated to give to the American-cockroach-extract medicinal extract for the treatment of tinea pedis G,；The auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis liniment or paint, you can the medicine for the treatment of tinea pedis is made.There is significant therapeutic effect to curing tinea pedis.
Description
Technical field
The present invention relates to a kind of process for preparing medicine, more particularly to a kind of process for preparing medicine for treating tinea pedis.
Background technology
Tinea pedis is a kind of chronic disease common in our daily lifes, and the people for having half inside average ten people has tinea pedis.
Tinea pedis is not only horrible but also is difficult to effect a radical cure, and troubles many patients always.Tinea pedis is generally divided into three types：Erosive type,
Vesicle, keratinization type.
Erosive type：The the 3rd and the 4th is apt to occur in, between the 4th and the 5th toe.Moist between toe, dipping turns white or a small water
Blister, dry up furfur after, peel off scurf for moistening, the rotten to the corn face of flush, have and very itch, easy scabies secondary infection.
Vesicle：It is apt to occur in foot edge portion.From the beginning of for the full phlysis of wall thickness, what is had is fused into bleb, and blister liquid is transparent,
Surrounding is without blush.Feel and very itch, often cause erysipelas, angioleucitis etc. after scratching because of scabies secondary infection.
Keratinization type：It is apt to occur in heel.It is mainly shown as skin thick thick and dries, angling furfur, itch, easily chaps.
For this type without blister and suppuration, the course of disease is slow, is not cured for many years.
American cockroach is Insecta Pterigota Blattaria Blattidae Periplaneta insect, is commonly called as cockroach, is body in Blattidae
The maximum insect of product, originates in South America.American cockroach is traditional Chinese medicine, be used as medicine first recorded in《Sheng Nong's herbal classic》, call " taste
It is salty, tremble with fear.Main blood stasis, disease heavily fortified point, fever and chills, break accumulation, and laryngopharynx is fought, interior cold, s.m.p ", to treat infantile malnutrition, furunculosis, carbuncle swells, larynx
Moth, nameless sores or boils, syphilis and poisonous snake, Wu pines such as are bitten at the disease.Because effective ingredient is many existing for American cockroach, commonly carry
Taking mode, its effect can not reach optimal for specified disease, therefore needs are special to American cockroach progress according to different diseases
Extraction, so that its therapeutic effect to certain disease reaches optimal.
The content of the invention
It is an object of the present invention to provide a kind of medicine for treating tinea pedis and preparation method thereof, the U.S. obtained using the preparation method
The big Lian active ingredients in continent have significant therapeutic effect to tinea pedis.
The present invention realizes above-mentioned purpose using following preparation method：
A, by dry American cockroach polypide cross 10-40 mesh sieve crush, by gained American cockroach coarse powder concentration be 60%~
95% ethanol immersion, ethanol liquid level is set to be higher by 5~10cm of coarse powder liquid level, closed preservation one is more than week, less than 18 months,
Coarse powder mixed liquor is made；
B, coarse powder mixed liquor obtained by step A is subjected to 3~5 ultrasonic extractions at a temperature of 30~50 DEG C, ultrasonic power is：
20-30KHz, when to coarse powder mixed liquor extract for the first time, the ethanol of 5~8 times of amount concentration 60%~95% of overstriking powder weight, carry
It is 30~60min to take the time；The ethanol of 3~6 times of amount concentration 60%~95% of overstriking powder weight, extraction are extracted after second every time
Time is 2~50min；Merge extract solution, filter to obtain American cockroach polypide ethanol extract；
C, ethanol extract obtained by step B is depressurized under conditions of vacuum -0.05~-0.06MPa, 50~60 DEG C of temperature dense
Contracting, to proportion be 1.05~1.15 when, obtain American cockroach polypide ethanol concentrate；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7~10 times of amounts of its weight, rise while stirring D,
Temperature is to 85~95 DEG C, then constant temperature stirs 10~60 minutes, and mixing speed is 20~60 revs/min, and then insulation stands 1~5 hour
It is allowed to be layered；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
0.5 ~ 1.0 times of amount medicinal carbon of gained ethanol concentrate stirs 30 ~ 60 minutes at a temperature of 40 ~ 60 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first steamed with 10 ~ 30 times of amounts of medicinal carbon
Distilled water is washed, and water lotion discards；Again with the eluent of 30 ~ 60 times of amounts of medicinal carbon, eluent is collected, through filter paper mistake
Filter, must elute filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa,
Be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis liniment or painting
Agent, you can the medicine for the treatment of tinea pedis is made.
Preferably, step B is extracted in accordance with the following methods, and coarse powder mixed liquor obtained by step A is carried out into ultrasonic extraction 3 times：
First time ultrasonic extraction：30~35 DEG C, ultrasonic power 20-25KHz of temperature, 5~8 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 30~60min；
Second of ultrasonic extraction：35~40 DEG C, ultrasonic power 23-28KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 20~50min；
Third time ultrasonic extraction：40~50 DEG C, ultrasonic power 25-30KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 35~50min；
It is highly preferred that in step B, first time ultrasonic extraction：30~32 DEG C, ultrasonic power 20-22KHz of temperature, overstriking powder weight 5
The ethanol of~8 times of amount concentration 60%~95%, extraction time is 35~45min.
It is highly preferred that in step B, second of ultrasonic extraction：35~38 DEG C, ultrasonic power 23-25KHz of temperature, overstriking powder
The ethanol of 3~6 times of amount concentration 75%~95% of weight, extraction time is 35~45min.
It is highly preferred that in step B, third time ultrasonic extraction：43~45 DEG C, ultrasonic power 28-30KHz of temperature, overstriking powder
3~6 times of amount concentration 80-95% of weight ethanol, extraction time is 45~50min.
It is highly preferred that step D：American cockroach polypide ethanol concentrate obtained by step C is added into 7~10 times of amounts of its weight
Purified water, warming while stirring is to 85~90 DEG C, then constant temperature stirs 45~60 minutes, and mixing speed is 45~60 revs/min, then
Insulation stands 4~5 hours and is allowed to be layered.
It is highly preferred that in step E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters,
0.5-0.8 times of filtrate of addition is measured medicinal carbon and stirred 45 minutes at a temperature of 50 DEG C in gained filtrate, it is fully adsorbed；
It is highly preferred that in step F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, decoction is released, first with medicinal work
Property 20 times of charcoal amount distillation water washings, water lotion discards；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected,
Filtered through filter paper, filtrate must be eluted, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
Beneficial effect：
1st, it is low that insoluble object amount in the obtained American-cockroach-extract medicinal extract water for the treatment of tinea pedis is extracted using income approach of the present invention
In 3%, total amino acid content is higher than 60.0%, is higher than 45% with reference to amino acid content.
2nd, the medicine being prepared using the method for the invention is put on the skin in tinea pedis affected part, and there is preferably treatment to imitate to tinea pedis
Fruit.
3rd, technique of the present invention extraction production temperature is relatively low, can effectively protect to the effective American cockroach of tinea pedis into
Part, lift tinea pedis cure rate.
Specific embodiment
Embodiment 1
The process for preparing medicine of extraction treatment tinea pedis, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 10 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 60%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 5cm, closed to preserve 18 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to 3 ultrasonic extractions at a temperature of 30 DEG C, ultrasonic power is：20KHz is right
When coarse powder mixed liquor extract for the first time, the ethanol of 5 times of amount concentration 60% of overstriking powder weight, extraction time 30min；Second
The ethanol of 3 times of amount concentration 60% of overstriking powder weight, extraction time 2min are extracted after secondary every time；Merge extract solution, filter beautiful
The big Lian polypides ethanol extract in continent；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.05MPa, temperature 50 C, is to proportion
When 1.05, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 10 minutes, mixing speed is 20 revs/min, and then insulation stands and is allowed to be layered for 1 hour；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
0.5 times of amount medicinal carbon of gained ethanol concentrate stirs 30 minutes at a temperature of 40 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 10 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 30 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of temperature 50 C, vacuum -0.05MPa, being concentrated into proportion is
When 1.20, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis liniment, you can
The medicine for the treatment of tinea pedis is made.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment tinea pedis for extracting to obtain using income approach of the present invention
1.2%, total amino acid content 69.3%, with reference to amino acid content 53.7%.
Case：
Erosive beriberi 30, man 15, female 15 are age 35-55 year, average（45±3.5）In year, the course of disease was at -5 years 1 year.It is main
Want symptom：Moist between toe, dipping turns white or a phlysis, after the furfur that dries up, peels off scurf as moistening, the rotten to the corn face of flush, has
Very itch, easy scabies secondary infection.
Treatment method：Patient did not received treating beri-beri in 2 months before the treatment, is wiping with before this medicine, is puncturing blister, wash
Remove blister liquid, by the American-cockroach-extract medicinal extract 0.2g of the treatment tinea pedis prepared by the present embodiment step G it is auxiliary in pharmaceutically may be used
Liniment 10ml is made in the carrier of receiving.Every natural gift morning, noon, evening, spray was wiped in affected part three times, is even sprayed and is wiped 2 months.
Criterion of therapeutical effect（1）It is effective：Moist between patient's toe, dipping turns white, play phlysis symptom, erosion, strange symptom of itching are complete
Disappear；（3）Effectively：Moist between patient's toe, dipping turns white, play phlysis symptom, erosion, strange symptom of itching have taken a turn for the better；（3）Nothing
Effect：Each symptom of patient is not improved.
Treatment results：Effective 20, effective 5, invalid 5, in therapeutic process, patient has no adverse reaction, after half a year
Pay a return visit without recurrence.
Embodiment 2
The process for preparing medicine of extraction treatment tinea pedis, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 40 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 95%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 10cm, closed to preserve 7 days, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to 5 ultrasonic extractions at a temperature of 50 DEG C, ultrasonic power is：30KHz is right
When coarse powder mixed liquor extract for the first time, the ethanol of 8 times of amount concentration 95% of overstriking powder weight, extraction time 60min；Second
The ethanol of 6 times of amount concentration 95% of overstriking powder weight, extraction time 50min are extracted after secondary every time；Merge extract solution, filter
American cockroach polypide ethanol extract；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.06MPa, temperature 60 C, is to proportion
When 1.15, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 10 times of amounts of its weight, warming while stirring D,
To 95 DEG C, then constant temperature stirs 60 minutes, and mixing speed is 60 revs/min, and then insulation stands 5 hours and is allowed to be layered；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
1.0 times of amount medicinal carbons of gained ethanol concentrate stir 60 minutes at a temperature of 60 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 30 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 60 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of temperature 60 C, vacuum -0.06MPa, being concentrated into proportion is
When 1.30, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis paint, you can
The medicine for the treatment of tinea pedis is made.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment tinea pedis for extracting to obtain using income approach of the present invention
0.7%, total amino acid content 71.4%, with reference to amino acid content 57.8%.
Case：
Vesicle tinea pedis 30, man 15, female 15 are age 45-55 year, average（48±3.5）In year, the course of disease was at -10 years 5 years.
Cardinal symptom：Foot edge portion is from the beginning of for the full phlysis of wall thickness, and what is had is fused into bleb, and blister liquid is transparent, around without blush, from
Feel is very itched, and often causes erysipelas, angioleucitis etc. after scratching because of scabies secondary infection.
Treatment method：Patient did not received treating beri-beri in 2 months before the treatment, is wiping with before this medicine, is puncturing blister, wash
Remove blister liquid, by the American-cockroach-extract medicinal extract 0.2g of the treatment tinea pedis prepared by the present embodiment step G it is auxiliary in pharmaceutically may be used
Paint 10ml is made in the carrier of receiving.Every natural gift morning, noon, evening, spray was wiped in affected part three times, is even sprayed and is wiped 2 months.
Criterion of therapeutical effect（1）It is effective：Do not recur phlysis, strange symptom of itching of patient's foot edge portion is wholly absent；（3）Effectively：Patient
Do not recur phlysis, strange symptom of itching of foot edge portion has taken a turn for the better；（3）It is invalid：Each symptom of patient is not improved.
Treatment results：Effective 21, effective 5, invalid 4, in therapeutic process, patient has no adverse reaction, after half a year
Pay a return visit without recurrence.
Embodiment 3
The process for preparing medicine of extraction treatment tinea pedis, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 20 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 75%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 8cm, closed to preserve 12 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：30 DEG C, ultrasonic power 20KHz of temperature, the ethanol of 5 times of overstriking powder weight amount concentration 75%, during extraction
Between be 30min；
Second of ultrasonic extraction：35 DEG C, ultrasonic power 23KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 75%, during extraction
Between be 20min；
Third time ultrasonic extraction：40 DEG C, ultrasonic power 25KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 75%, during extraction
Between be 35min；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.05MPa, temperature 50 C, is to proportion
When 1.05, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 45 minutes, mixing speed is 45 revs/min, and then insulation stands and is allowed to be layered for 4 hours.
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, added in gained filtrate
0.7 times of amount medicinal carbon of ethanol concentrate obtained by step C stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 45 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 55 DEG C of temperature, vacuum -0.06MPa, being concentrated into proportion is
When 1.25, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis liniment, you can
The medicine for the treatment of tinea pedis is made.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment tinea pedis for extracting to obtain using income approach of the present invention
1.0%, total amino acid content 72.1%, with reference to amino acid content 58.0%.
Case：
Keratinization type tinea pedis 30, man 15, female 15 are age 30-65 year, average（47±6.5）In year, the course of disease was at -15 years 5 years.
Cardinal symptom：Skin of heel is thick thick and dries, angling furfur, itch, easily chaps, no blister and suppuration, the course of disease is slow, more
Nian Buyu.
Treatment method：Patient did not received treating beri-beri in 2 months before the treatment, was wiping with before this medicine, wash clean pin will
The American-cockroach-extract medicinal extract 0.2g for the treatment of tinea pedis prepared by the present embodiment step G it is auxiliary in pharmaceutically acceptable carrier
Liniment 10ml is made.Every natural gift morning, noon, evening, spray was wiped in affected part three times, is even sprayed and is wiped 2 months.
Criterion of therapeutical effect（1）It is effective：Skin of heel becomes fine and smooth no chap, and pruritis disappears；（3）Effectively：Skin of heel
Thick thickness is dried, angling furfur, itch, and the symptom such as chap has taken a turn for the better；（3）It is invalid：Each symptom of patient is not improved.
Treatment results：Effective 23, effective 4, invalid 3, in therapeutic process, patient has no adverse reaction, after half a year
Pay a return visit without recurrence.
Embodiment 4
The process for preparing medicine of extraction treatment tinea pedis, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 30 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 85%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 10cm, closed to preserve 10 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：35 DEG C, ultrasonic power 25KHz of temperature, the ethanol of 8 times of overstriking powder weight amount concentration 85%, during extraction
Between be 60min；
Second of ultrasonic extraction：40 DEG C, ultrasonic power 28KHz of temperature, the ethanol of 6 times of overstriking powder weight amount concentration 85%, during extraction
Between be 50min；
Third time ultrasonic extraction：Temperature 50 C, ultrasonic power 30KHz, the ethanol of 6 times of overstriking powder weight amount concentration 85%, during extraction
Between be 50min；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.06MPa, temperature 60 C, is to proportion
When 1.15, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 10 times of amounts of its weight, warming while stirring D,
To 90 DEG C, then constant temperature stirs 60 minutes, and mixing speed is 60 revs/min, and then insulation stands 5 hours and is allowed to be layered.
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, added in gained filtrate
0.8 times of amount medicinal carbon of filtrate stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 55 DEG C of temperature, vacuum -0.05MPa, is concentrated into ratio
When weight is 1.20, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis paint, you can
The medicine for the treatment of tinea pedis is made.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment tinea pedis for extracting to obtain using income approach of the present invention
0.9%, total amino acid content 66.3%, with reference to amino acid content 51.7%.
Case：
Erosive beriberi 30, man 15, female 15 are age 33-58 year, average（43±7.5）In year, the course of disease was at -15 years 10 years.
Cardinal symptom：Moist between toe, dipping turns white or a phlysis, after the furfur that dries up, peels off scurf as moistening, the rotten to the corn face of flush,
Have and very itch, easy scabies secondary infection.
Treatment method：Patient did not received treating beri-beri in 2 months before the treatment, is wiping with before this medicine, is puncturing blister, wash
Remove blister liquid, by the American-cockroach-extract medicinal extract 0.2g of the treatment tinea pedis prepared by the present embodiment step G it is auxiliary in pharmaceutically may be used
Paint 10ml is made in the carrier of receiving.Every natural gift morning, noon, evening, spray was wiped in affected part three times, is even sprayed and is wiped 2 months.
Criterion of therapeutical effect（1）It is effective：Moist between patient's toe, dipping turns white, play phlysis symptom, erosion, strange symptom of itching are complete
Disappear；（3）Effectively：Moist between patient's toe, dipping turns white, play phlysis symptom, erosion, strange symptom of itching have taken a turn for the better；（3）Nothing
Effect：Each symptom of patient is not improved.
Treatment results：Effective 24, effective 3, invalid 3, in therapeutic process, patient has no adverse reaction, after half a year
Pay a return visit without recurrence.
Embodiment 5
The process for preparing medicine of extraction treatment tinea pedis, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 30 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 90%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 5cm, closed to preserve 3 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：30 DEG C, ultrasonic power 20KHz of temperature, the ethanol of 5 times of overstriking powder weight amount concentration 90%, during extraction
Between be 355min；
Second of ultrasonic extraction：35 DEG C, ultrasonic power 23KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 90%, during extraction
Between be 35min；
Third time ultrasonic extraction：43 DEG C, ultrasonic power 28KHz of temperature, the ethanol of 3 times of overstriking powder weight amount concentration 90%, during extraction
Between be 45min；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.05MPa, temperature 50 C, is to proportion
When 1.05, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 10 minutes, mixing speed is 20 revs/min, and then insulation stands and is allowed to be layered for 1 hour；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, filtrate is added in gained filtrate
0.5 times of amount medicinal carbon stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
G, elution filtrate obtained by step F is depressurized under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa dense
Contracting, be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis liniment, you can
The medicine for the treatment of tinea pedis is made.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment tinea pedis for extracting to obtain using income approach of the present invention
1.7%, total amino acid content 68.2%, with reference to amino acid content 56.1%.
Case：
Vesicle tinea pedis 30, man 15, female 15 are age 39-59 year, average（42±5.5）In year, the course of disease was at -15 years 8 years.
Cardinal symptom：Foot edge portion is from the beginning of for the full phlysis of wall thickness, and what is had is fused into bleb, and blister liquid is transparent, around without blush, from
Feel is very itched, and often causes erysipelas, angioleucitis etc. after scratching because of scabies secondary infection.
Treatment method：Patient did not received treating beri-beri in 2 months before the treatment, is wiping with before this medicine, is puncturing blister, wash
Remove blister liquid, by the American-cockroach-extract medicinal extract 0.2g of the treatment tinea pedis prepared by the present embodiment step G it is auxiliary in pharmaceutically may be used
Paint 10ml is made in the carrier of receiving.Every natural gift morning, noon, evening, spray was wiped in affected part three times, is even sprayed and is wiped 2 months.
Criterion of therapeutical effect（1）It is effective：Do not recur phlysis, strange symptom of itching of patient's foot edge portion is wholly absent；（3）Effectively：Patient
Do not recur phlysis, strange symptom of itching of foot edge portion has taken a turn for the better；（3）It is invalid：Each symptom of patient is not improved.
Treatment results：Effective 24, effective 6, invalid 0, in therapeutic process, patient has no adverse reaction, after half a year
Pay a return visit without recurrence.
Embodiment 6
The process for preparing medicine of extraction treatment tinea pedis, is realized using following technology from American cockroach：
A, dry American cockroach polypide is crossed into 20 mesh sieve to crush, by the ethanol that gained American cockroach coarse powder concentration is 90%
Immersion, ethanol liquid level is set to be higher by coarse powder liquid level 10cm, closed to preserve 15 months, obtained coarse powder mixed liquor；
B, coarse powder mixed liquor obtained by step A is subjected to ultrasonic extraction 3 times：
First time ultrasonic extraction：32 DEG C, ultrasonic power 22KHz of temperature, the ethanol of 8 times of overstriking powder weight amount concentration 90%, during extraction
Between be 45min；
Second of ultrasonic extraction：38 DEG C, ultrasonic power 23-25KHz of temperature, the ethanol of 6 times of amount concentration 90% of overstriking powder weight, is carried
It is 45min to take the time；
Third time ultrasonic extraction：Temperature 45 C, ultrasonic power 28-30KHz, the ethanol of 6 times of amount concentration 90% of overstriking powder weight, carry
It is 50min to take the time；
C, ethanol extract obtained by step B is concentrated under reduced pressure under conditions of vacuum -0.06MPa, temperature 60 C, is to proportion
When 1.15, American cockroach polypide ethanol concentrate is obtained；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 8 times of amounts of its weight, warming while stirring is extremely D,
85 DEG C, then constant temperature stirs 50 minutes, mixing speed is 50 revs/min, and then insulation stands and is allowed to be layered for 5 hours.
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, added in gained filtrate
0.6 times of amount medicinal carbon of ethanol concentrate obtained by step C stirs 45 minutes at a temperature of 50 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first with 20 times of amount distilled water of medicinal carbon
Washing, water lotion discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, filters, must elute through filter paper
Filtrate, it is water that the eluant, eluent counts proportioning in parts by weight：Ethyl acetate：95% ethanol=3：2：2；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa,
Be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G is made into tinea pedis paint, you can
The medicine for the treatment of tinea pedis is made.
Insoluble object amount in the American-cockroach-extract medicinal extract water for the treatment tinea pedis for extracting to obtain using income approach of the present invention
1.2%, total amino acid content 69.5%, with reference to amino acid content 52.8%.
Case：
Keratinization type tinea pedis 30, man 15, female 15 are age 41-58 year, average（47±2.5）In year, the course of disease was at -30 years 15 years.
Cardinal symptom：Skin of heel is thick thick and dries, angling furfur, itch, easily chaps, no blister and suppuration, the course of disease is slow, more
Nian Buyu.
Treatment method：Patient did not received treating beri-beri in 2 months before the treatment, was wiping with before this medicine, wash clean pin will
The American-cockroach-extract medicinal extract 0.2g for the treatment of tinea pedis prepared by the present embodiment step G it is auxiliary in pharmaceutically acceptable carrier
Paint 10ml is made.Every natural gift morning, noon, evening, spray was wiped in affected part three times, is even sprayed and is wiped 2 months.
Criterion of therapeutical effect（1）It is effective：Skin of heel becomes fine and smooth no chap, and pruritis disappears；（3）Effectively：Skin of heel
Thick thickness is dried, angling furfur, itch, and the symptom such as chap has taken a turn for the better；（3）It is invalid：Each symptom of patient is not improved.
Treatment results：Effective 25, effective 5, invalid 0, in therapeutic process, patient has no adverse reaction, after half a year
Pay a return visit without recurrence.
Claims (9)
1. American cockroach is preparing the application in treating beriberi.
2. a kind of process for preparing medicine for treating tinea pedis, it is characterised in that prepared according to following steps：
A, by dry American cockroach polypide cross 10-40 mesh sieve crush, by gained American cockroach coarse powder concentration be 60%~
95% ethanol immersion, ethanol liquid level is set to be higher by 5~10cm of coarse powder liquid level, closed preservation one is more than week, less than 18 months,
Coarse powder mixed liquor is made；
B, coarse powder mixed liquor obtained by step A is subjected to 3~5 ultrasonic extractions at a temperature of 30~50 DEG C, ultrasonic power is：
20-30KHz, when to coarse powder mixed liquor extract for the first time, the ethanol of 5~8 times of amount concentration 60%~95% of overstriking powder weight, carry
It is 30~60min to take the time；The ethanol of 3~6 times of amount concentration 60%~95% of overstriking powder weight, extraction are extracted after second every time
Time is 2~50min；Merge extract solution, filter to obtain American cockroach polypide ethanol extract；
C, ethanol extract obtained by step B is depressurized under conditions of vacuum -0.05~-0.06MPa, 50~60 DEG C of temperature dense
Contracting, to proportion be 1.05~1.15 when, obtain American cockroach polypide ethanol concentrate；
American cockroach polypide ethanol concentrate obtained by step C is added to the purified water of 7~10 times of amounts of its weight, rise while stirring D,
Temperature is to 85~95 DEG C, then constant temperature stirs 10~60 minutes, and mixing speed is 20~60 revs/min, and then insulation stands 1~5 hour
It is allowed to be layered；
E, lower floor's aqueous obtained by step D is released, collects lower floor's aqueous, after filter paper filters, step C is added in gained filtrate
0.5 ~ 1.0 times of amount medicinal carbon of gained ethanol concentrate stirs 30 ~ 60 minutes at a temperature of 40 ~ 60 DEG C, it is fully adsorbed；
F, decoction containing activated carbon obtained by step E is fitted into chromatographic column, releases decoction, first steamed with 10 ~ 30 times of amounts of medicinal carbon
Distilled water is washed, and water lotion discards；Again with the eluent of 30 ~ 60 times of amounts of medicinal carbon, eluent is collected, through filter paper mistake
Filter, must elute filtrate；
G, elution filtrate obtained by step F is concentrated under reduced pressure under the conditions of 50~60 DEG C of temperature, vacuum -0.05~-0.06MPa,
Be concentrated into proportion for 1.20~1.30 when, the American-cockroach-extract medicinal extract of tinea pedis must be treated；
H, the auxiliary pharmaceutically acceptable carrier of American-cockroach-extract medicinal extract obtained by step G will be made tinea pedis liniment or painting
Agent, you can the medicine for the treatment of tinea pedis is made.
3. a kind of process for preparing medicine for treating tinea pedis according to claim 2, it is characterised in that step B is according to following
Method is extracted, and coarse powder mixed liquor obtained by step A is carried out into ultrasonic extraction 3 times：
First time ultrasonic extraction：30~35 DEG C, ultrasonic power 20-25KHz of temperature, 5~8 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 30~60min；
Second of ultrasonic extraction：35~40 DEG C, ultrasonic power 23-28KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 20~50min；
Third time ultrasonic extraction：40~50 DEG C, ultrasonic power 25-30KHz of temperature, 3~6 times of amount concentration 60% of overstriking powder weight~
95% ethanol, extraction time are 35~50min.
4. a kind of process for preparing medicine for treating tinea pedis according to claim 3, it is characterised in that in step B, for the first time
Ultrasonic extraction：30~32 DEG C, ultrasonic power 20-22KHz of temperature, the ethanol of 5~8 times of amount concentration 60%~95% of overstriking powder weight,
Extraction time is 35~45min.
A kind of 5. process for preparing medicine for treating tinea pedis according to claim 3, it is characterised in that in step B, second
Ultrasonic extraction：35~38 DEG C, ultrasonic power 23-25KHz of temperature, the ethanol of 3~6 times of amount concentration 75%~95% of overstriking powder weight,
Extraction time is 35~45min.
A kind of 6. process for preparing medicine for treating tinea pedis according to claim 3, it is characterised in that in step B, third time
Ultrasonic extraction：43~45 DEG C, ultrasonic power 28-30KHz of temperature, 3~6 times of amount concentration 80-95% of overstriking powder weight ethanol, is carried
It is 45~50min to take the time.
A kind of 7. process for preparing medicine for treating tinea pedis according to claim 2, it is characterised in that step D：By step C
Gained American cockroach polypide ethanol concentrate adds the purified waters of 7~10 times of its weight amount, warming while stirring to 85~90 DEG C,
Constant temperature stirs 45~60 minutes again, and mixing speed is 45~60 revs/min, and then insulation stands 4~5 hours and is allowed to be layered.
8. a kind of process for preparing medicine for treating tinea pedis according to claim 2, it is characterised in that in step E, by step
Lower floor's aqueous is released obtained by D, collects lower floor's aqueous, and after filter paper filters, 0.5-0.8 times of filtrate of addition measures medicine in gained filtrate
Stirred 45 minutes at a temperature of 50 DEG C with activated carbon, it is fully adsorbed.
9. a kind of process for preparing medicine for treating tinea pedis according to claim 1, it is characterised in that in step F, by step
Decoction containing activated carbon obtained by E is fitted into chromatographic column, releases decoction, first with 20 times of amount distillation water washings of medicinal carbon, water lotion
Discard；Again with the eluent of 50 times of amounts of medicinal carbon, eluent is collected, is filtered through filter paper, filtrate must be eluted, it is described to wash
It is water that proportioning is counted in de- agent in parts by weight：Ethyl acetate：95% ethanol=3：2：2.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710227231X | 2017-04-10 | ||
| CN201710227231 | 2017-04-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107582571A true CN107582571A (en) | 2018-01-16 |
Family
ID=61053299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710946037.7A Withdrawn CN107582571A (en) | 2017-04-10 | 2017-10-12 | A kind of medicine for treating tinea pedis and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107582571A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111135218A (en) * | 2020-02-19 | 2020-05-12 | 海南森祺制药有限公司 | Nauclea officinalis extract and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988420A (en) * | 2012-08-23 | 2013-03-27 | 耿福能 | Nano periplaneta americana product as well as preparation method and application thereof |
| CN104042635A (en) * | 2013-03-14 | 2014-09-17 | 成都百草和济科技有限公司 | Tablet of cockroach medicine and preparation method thereof |
| CN106265754A (en) * | 2016-10-13 | 2017-01-04 | 昆明赛诺制药股份有限公司 | A kind of method that a chain of extraction obtains two kinds of effective ingredient from periplaneta americana |
-
2017
- 2017-10-12 CN CN201710946037.7A patent/CN107582571A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988420A (en) * | 2012-08-23 | 2013-03-27 | 耿福能 | Nano periplaneta americana product as well as preparation method and application thereof |
| CN104042635A (en) * | 2013-03-14 | 2014-09-17 | 成都百草和济科技有限公司 | Tablet of cockroach medicine and preparation method thereof |
| CN106265754A (en) * | 2016-10-13 | 2017-01-04 | 昆明赛诺制药股份有限公司 | A kind of method that a chain of extraction obtains two kinds of effective ingredient from periplaneta americana |
Non-Patent Citations (1)
| Title |
|---|
| 满红霞 等: "药用昆虫美洲大蠊化学成分及其药理作用的研究进展", 《抗感染药学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111135218A (en) * | 2020-02-19 | 2020-05-12 | 海南森祺制药有限公司 | Nauclea officinalis extract and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106265754A (en) | A kind of method that a chain of extraction obtains two kinds of effective ingredient from periplaneta americana | |
| CN112409504B (en) | Preparation method and application of Sipunculus nudus polysaccharide | |
| CN105231450B (en) | A kind of pumpkin lozenge and preparation method thereof | |
| CN108938800A (en) | It is a kind of to treat skin disease painting liquid film | |
| CN115531448A (en) | Francisella bark extract powder, preparation thereof and application thereof in stomach bismuth magnesium particles | |
| CN107582571A (en) | A kind of medicine for treating tinea pedis and preparation method thereof | |
| CN105267811A (en) | Externally-used granule treating manus and tinea pedis, and preparation method and application thereof | |
| CN104491359B (en) | It is a kind of to treat canker sore, Chinese medicine composition that pharyngitis swells and ache and preparation method thereof | |
| CN107638429A (en) | A kind of medicine for treating rhinitis and preparation method thereof | |
| CN107648273A (en) | A kind of medicine for treating canker sore and preparation method thereof | |
| CN106581077A (en) | Preparing method of medicine for treating hepatitis B virus carrier | |
| CN106166165A (en) | A kind of American cockroach medicament composition being effectively improved various clinical treating correlative diseases effect and preparation method thereof | |
| CN107684565A (en) | A kind of medicine for treating blood-shoot-eye illness and preparation method thereof | |
| CN104606559B (en) | A kind of Camellia Leaves antipruritic | |
| CN103735682A (en) | Drug for treating allergic dermatitis and preparation method thereof | |
| CN113332365B (en) | Compound lithospermum ointment and preparation method thereof | |
| KR100902679B1 (en) | Prepartaion of concentrated syrups for oral use containing alcohol fermentated extracts of zingiberis rhizoma and its compounding herbal medicines | |
| CN108478614A (en) | A kind of preparation method of XIAOAIPING PIAN | |
| CN107648272A (en) | A kind of medicine for treating reflux esophagitis and preparation method thereof | |
| WO2019071658A1 (en) | Traditional chinese medicine composition treating pulmonary disease, preparation method and application | |
| CN110478420B (en) | Outdoor protective oil and preparation method thereof | |
| CN106728897A (en) | A kind of Chinese medicine composition for treating frush rot | |
| CN107550939A (en) | A kind of medicine for treating diabetes and preparation method thereof | |
| CN107582572A (en) | One kind treats cirsoid medicine and preparation method thereof | |
| CN104689014A (en) | Preparation method of pleural lavage fluid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180116 |
|
| WW01 | Invention patent application withdrawn after publication |