CN107581461A - A kind of preparation method of high whiteness Peru squid skin gelatin - Google Patents
A kind of preparation method of high whiteness Peru squid skin gelatin Download PDFInfo
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- CN107581461A CN107581461A CN201710877641.9A CN201710877641A CN107581461A CN 107581461 A CN107581461 A CN 107581461A CN 201710877641 A CN201710877641 A CN 201710877641A CN 107581461 A CN107581461 A CN 107581461A
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- gelatin
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Abstract
The present invention relates to a kind of gelatin preparation method, specifically provides a kind of preparation method of high whiteness Peru squid skin gelatin, it is characterised in that comprise the following steps：(1) pretreatment of raw material；(2) degreasing and foreign protein is removed；(3) decolourize：The squid skin for being washed till neutrality is placed in Na2CO3In solution, 1.5 2h are stirred at room temperature, until Na2CO3Untill solution colour is shallow to constant, every 15 25min changes liquid once；(4) mineral matter is removed to neutrality；(5) gelatin extraction；(6) filter, dry.The preparation method of this high whiteness Peru squid skin gelatin provided by the invention, technology controlling and process is simple, easily operated, and decolorizing efficiency is high, and gelatin loss late is low；Obtained gelatin is of light color, and whiteness is higher, substantially increases the economic value of gelatin；Safe and non-toxic, production cost is low, is easy to industrialized production.
Description
Technical field
The present invention relates to a kind of gelatin preparation method, and in particular to a kind of preparation side of high whiteness Peru squid skin gelatin
Method.
Background technology
Gelatin (Gelatin) is a kind of soluble natural polymer polypeptide polymer, is collagen through gentle and irreversible
Post-rift primary product.Because of its unique physicochemical characteristics and feature, by as gelling agent, thickener, emulsifying agent, fill out
Agent, stabilizer, adhesive, foaming agent and fining agent etc. are filled, is widely used in chemical industry, food, medicine, defends the fields such as adornment.China
Squid aboundresources, squid skin accounts for 10% of accessory substance or so in squid deep-processing process, the current extraction work of squid skin gelatin
Skill includes alkaline extraction, sour formulation, hot-water extraction and enzyme formulation, but due to containing a certain amount of pigment in squid skin, extraction obtains
Gelatin be generally kermesinus or pink, make its application be very restricted.Therefore, squid skin must be carried out at decolouring
Reason, to improve the whiteness of product.
It is at present charcoal absorption and hydrogen peroxide for decoloration using more decoloration treatment method in production, but it is cumbersome,
Time is longer, and decolorizing effect is undesirable, and activated carbon is easily mixed into gelatin, is very difficult to remove totally, influences further adding for gelatin
Work.
For example, China Patent Publication No. CN103060407A, publication date on April 24th, 2013, a kind of high transparency is disclosed
Spend the preparation technology of squid skin collagen.This method using the squid skin of one of marine products processing discarded object as raw material, using EDTA with
The discoloration method of sodium polyphosphate chelating joint hydrogen peroxide prepares the shallower collagen of color.Weak point is：Hydrogen peroxide
Utilization rate in decolorization is relatively low, and percent of decolourization 82% or so, obtained collagen is in pale pink, and being unfavorable for production should
With.
The content of the invention
The purpose of the present invention is the defects of overcoming prior art, there is provided a kind of preparation side of high whiteness Peru squid skin gelatin
Method, obtained squid skin gelatin whiteness is high, has a wide range of application, and economic value is high.
The technical solution adopted in the present invention is：A kind of preparation method of high whiteness Peru squid skin gelatin, including it is as follows
Step：
(1) pretreatment of raw material：Squid skin is cleaned with distilled water and removes surface impurity, is freezed after being then cut into fragment
72 hours are dried with standby；
(2) degreasing and foreign protein is removed：Dried squid skin is placed in 0.05-0.10mol/L NaOH solutions, in
Magnetic agitation 3-5h at room temperature, solution is finally washed till neutrality with distilled water；
Safe and non-toxic using NaOH solution degreasing, degreasing efficiency high, cost is low, goes removing protein more thorough, is advantageous to follow-up
The extraction of gelatin.
(3) decolourize：The squid skin for being washed till neutrality is placed in Na2CO3In solution, 1.5-2h is stirred at room temperature, until
Na2CO3Untill solution colour is shallow to constant, liquid is changed once per 15-25min；Using Na2CO3Solution carries out decolourizing to complete for the first time
Afterwards, it is 8.0 to adjust pH value of solution, adds complexing agent stirring 11-13h, squid is cleaned untill solution colour near-white, then with distilled water
Fish-skin is to neutrality.
(4) mineral matter is removed：It will be placed in by the squid skin to decolourize in 0.05-0.10mol/L HCl solutions, at room temperature magnetic
Power stirs 55-65min, changes liquid once per 25-35min, solution finally is washed till into neutrality with distilled water；
(5) gelatin extraction：With solid-liquid ratio 1: (20-40) adds squid skin in distilled water, is placed in thermostat water bath and carries
Take gelatin；
(6) filter, dry --- the gelatin solution extracted is placed in and cools down 30min at room temperature, by 150-200 mesh yarns
Cloth filters to obtain gelatin extraction liquid, and gelatin extraction liquid freeze-drying 72h obtains high whiteness Peru squid skin gelatin finished product.
Further, alkali lye is changed once per 30-60min during step (2) magnetic agitation.
Further, in the step (2), the NaOH solution dosage per 1g squid skins is 10-20mL.
Further, in the step (3), Na2CO3The concentration of solution is 0.4-0.6mol/L.
Further, in the step (3), add after the completion of complexing agent decolouring, in addition to step：Solution is heated to temperature
Spend for 45-55 DEG C, add the acidifying Concave-convex clay rod that mass fraction is 7.5%-8.5% and stir 55-65min, complete to decolourize.
Na2CO3, EDTA coordinate Concave-convex clay rod to be decolourized jointly, technology controlling and process is simple, easily operated, and decolorizing efficiency is high, decolourizes
Rate reaches 95% or so, and gelatin loss late is low；In addition, squid skin has very serious bad smell, Concave-convex clay rod can not only
Decolourize, can also deodorization taste removal, making obtained squid skin gelatin, not only color and luster is good, can part deodorant stink.
Further, the complexing agent is ethylenediamine tetra-acetic acid, concentration 0.2-0.4mol/L.
Further, in the step (4), the HCl solution dosage per 1g squid skins is 10-20mL.
Further, in the step (5), hot-water extraction gelatin, with 55 DEG C for initial extraction temperature, 5 DEG C are raised per 1h,
Hot-water extraction 4h to Extracting temperature be 70 DEG C.
Further, also include deodorant step after step (5) gelatin extraction, be specially：Squid skin gelatin is extracted
Liquid is placed in mass fraction in 4%-5% aqueous trehaloses, to soak 30-40min at room temperature.
After aqueous trehalose soaks, the bad smell of squid skin gelatin extract solution almost disappears；In addition, trehalose belongs to
Polysaccharide low in calories, add in squid skin gelatin extract solution, the gel strength of gelatin can be improved.
Further, in the step (6), drying mode is freeze-drying.Squid skin gelatin sense made from freeze-drying
Official is preferable, and yield is higher.
Beneficial effects of the present invention：The preparation method of this high whiteness Peru squid skin gelatin provided by the invention, technique
Control is simple, easily operated, and decolorizing efficiency is high, and gelatin loss late is low, and obtained gelatin whiteness is higher, color stability, is not easy to return
Color, there is good stability；It is high to extract obtained gelatin gel strength；Obtained gelatin is of light color, and whiteness is higher, carries significantly
The high economic value of gelatin；Safe and non-toxic, production cost is low, is easy to industrialized production.
Embodiment
With reference to embodiment, the present invention is furture elucidated.
Embodiment：
A kind of preparation method of high whiteness Peru squid skin gelatin is present embodiments provided, is comprised the following steps：
(1) pretreatment of raw material：Squid skin is cleaned with distilled water and removes surface impurity, is then cut into the broken of 1 × 1cm
72 hours are freeze-dried after piece with standby；
(2) degreasing and foreign protein is removed：Dried squid skin is placed in 0.05-0.10mol/L NaOH solutions, in
Magnetic agitation 3-5h at room temperature, alkali lye is changed once per 30-60min during magnetic agitation, is finally washed solution with distilled water
To neutral (pH 7.0-7.5)；Wherein, it is 10-20mL per the NaOH solution dosage of 1g squid skins.
Safe and non-toxic using NaOH solution degreasing, degreasing efficiency high, cost is low, goes removing protein more thorough, is advantageous to follow-up
The extraction of gelatin.
(3) decolourize：The squid skin for being washed till neutrality is placed in the Na that concentration is 0.4-0.6mol/L2CO3In solution (1), in room
1.5-2h is stirred under warm (20-25 DEG C), until Na2CO3Untill solution colour is shallow to constant, changes liquid once per 15-25min, complete
Decolourize for the first time；After the completion of decolourizing for the first time, regulation pH value of solution is 8.0, adds the complexing agent that concentration is 0.2-0.4mol/L and stirs
11-13h is mixed, squid skin solution 4-5 times is cleaned to neutral untill solution colour near-white, then with distilled water.Then again will be molten
Liquid is heated to temperature as 45-55 DEG C, adds the acidifying Concave-convex clay rod that mass fraction is 7.5%-8.5% and stirs 55-
65min, complete to decolourize.
(4) mineral matter is removed：It will be placed in by the squid skin to decolourize in 0.05-0.10mol/L HCl solutions, in room temperature (20-
25 DEG C) under magnetic agitation 55-65min, change liquid once per 25-35min, solution be finally washed till neutrality (pH 6.5- with distilled water
7.0)；HCl solution dosage per 1g squid skins is 10-20mL.
(5) gelatin extraction：With solid-liquid ratio 1: (20-40) adds squid skin in distilled water, is placed in thermostat water bath and carries
Take gelatin；Hot-water extraction gelatin, with 55 DEG C for initial extraction temperature, 5 DEG C are raised per 1h, hot-water extraction 4h to Extracting temperature is 70
℃。
(6) squid skin gelatin extract solution is placed in mass fraction in 4%-5% aqueous trehaloses, to soak at room temperature
30-40min。
(7) filter, dry --- the gelatin solution extracted is placed in and cools down 30min at room temperature, by 150-200 mesh yarns
Cloth filters to obtain gelatin extraction liquid, and gelatin extraction liquid freeze-drying 72h obtains high whiteness Peru squid skin gelatin finished product.Drying mode is
Freeze-drying.Squid skin gelatin sense organ is preferable made from freeze-drying, and yield is higher.
Blank is made with distilled water, determines the value of chromatism of gelatin extraction stoste and gelatin extraction liquid respectively using color difference meter, and
Δ E1, Δ E2 are designated as successively, and the calculation formula of percent of decolourization is：Percent of decolourization=(Δ E1- Δ E2) ÷ Δ E1 × 100%, percent of decolourization
15.01 × 100%=95.2% of=(15.01-0.72) ÷.
It is exemplified as above be only to the present invention for example, do not form the limitation to protection scope of the present invention, it is all
It is to be belonged to the same or analogous design of the present invention within protection scope of the present invention.
Claims (10)
1. a kind of preparation method of high whiteness Peru squid skin gelatin, it is characterised in that comprise the following steps：
(1) pretreatment of raw material：Squid skin is cleaned with distilled water and removes surface impurity, is freeze-dried after being then cut into fragment
72 hours with standby；
(2) degreasing and foreign protein is removed：Dried squid skin is placed in 0.05-0.10mol/L NaOH solutions, in room temperature
Lower magnetic agitation 3-5h, is finally washed till neutrality with distilled water by solution；
(3) decolourize：The squid skin for being washed till neutrality is placed in Na2CO3In solution, 1.5-2h is stirred at room temperature, until Na2CO3It is molten
Untill liquid is of light color to constant, liquid is changed once per 15-25min；Using Na2CO3After the completion of solution carries out first time decolouring, regulation
PH value of solution is 8.0, adds complexing agent stirring 11-13h, squid skin is cleaned extremely untill solution colour near-white, then with distilled water
It is neutral.
(4) mineral matter is removed：It will be placed in by the squid skin to decolourize in 0.05-0.10mol/L HCl solutions, magnetic force stirs at room temperature
55-65min is mixed, liquid is changed once per 25-35min, solution is finally washed till neutrality with distilled water；
(5) gelatin extraction：With solid-liquid ratio 1: (20-40) will squid skin add distilled water in, be placed in thermostat water bath extract it is bright
Glue；
(6) filter, dry --- the gelatin solution extracted is placed in and cools down 30min at room temperature, by 150-200 mesh gauze mistakes
Gelatin extraction liquid is filtered to obtain, gelatin extraction liquid freeze-drying 72h obtains high whiteness Peru squid skin gelatin finished product.
2. preparation method as claimed in claim 1, it is characterised in that：Per 30- during step (2) magnetic agitation
60min changes alkali lye once.
3. preparation method as claimed in claim 1, it is characterised in that：In the step (2), per the NaOH solution of 1g squid skins
Dosage is 10-20mL.
4. preparation method as claimed in claim 1, it is characterised in that：In the step (3), Na2CO3The concentration of solution is
0.4-0.6mol/L。
5. preparation method as claimed in claim 1, it is characterised in that：In the step (3), add complexing agent and decolourize to complete
Afterwards, in addition to step：Solution is heated to temperature as 45-55 DEG C, adds the acidifying concave convex rod that mass fraction is 7.5%-8.5%
Stone clay stirs 55-65min, completes to decolourize.
6. preparation method as claimed in claim 1, it is characterised in that：The complexing agent is ethylenediamine tetra-acetic acid, and concentration is
0.2-0.4mol/L。
7. preparation method as claimed in claim 1, it is characterised in that：In the step (4), per the HCl solution of 1g squid skins
Dosage is 10-20mL.
8. preparation method as claimed in claim 1, it is characterised in that：In the step (5), hot-water extraction gelatin, with 55 DEG C
For initial extraction temperature, 5 DEG C are raised per 1h, hot-water extraction 4h to Extracting temperature is 70 DEG C.
9. preparation method as claimed in claim 1, it is characterised in that：Also include deodorant after step (5) gelatin extraction to walk
Suddenly, it is specially：Squid skin gelatin extract solution is placed in mass fraction in 4%-5% aqueous trehaloses, to soak 30- at room temperature
40min。
10. preparation method as claimed in claim 1, it is characterised in that：In the step (6), drying mode is freeze-drying.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113185603A (en) * | 2021-05-10 | 2021-07-30 | 青岛海洋生物医药研究院 | Marine medical collagen with low immunogenicity, and preparation method and application thereof |
| CN117511417A (en) * | 2023-12-07 | 2024-02-06 | 中国海洋大学 | Method for rapidly extracting squid cartilage gelatin and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113185603A (en) * | 2021-05-10 | 2021-07-30 | 青岛海洋生物医药研究院 | Marine medical collagen with low immunogenicity, and preparation method and application thereof |
| CN117511417A (en) * | 2023-12-07 | 2024-02-06 | 中国海洋大学 | Method for rapidly extracting squid cartilage gelatin and application thereof |
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| CN107581461B (en) | 2020-11-10 |
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