CN107552115B - Detection device based on microfluidic technology and working method thereof - Google Patents
Detection device based on microfluidic technology and working method thereof Download PDFInfo
- Publication number
- CN107552115B CN107552115B CN201710585320.1A CN201710585320A CN107552115B CN 107552115 B CN107552115 B CN 107552115B CN 201710585320 A CN201710585320 A CN 201710585320A CN 107552115 B CN107552115 B CN 107552115B
- Authority
- CN
- China
- Prior art keywords
- disc
- magnetic
- detection
- groove
- force piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a detection device based on a microfluidic technology and a working method thereof, wherein the detection device comprises a first disc, the first disc is provided with a plurality of detection units, and the detection units comprise a detection groove and a waste liquid groove; a second disk disposed below the first disk; the second disc is provided with a plurality of magnetic units, the number of the magnetic units is equal to that of the detection units, and the magnetic units comprise limiting grooves and first magnetic parts; the included angle between the trend of the limit groove and the radial direction of the second disc is an acute angle, and the first magnetic force piece is arranged in the limit groove; the second magnetic force piece is fixed on the outer side of the end part, which is close to the center of the second disc, of the limiting groove; the second magnetic member remains relatively stationary with the second disk. The invention has the advantages of high sensitivity, short detection time and the like.
Description
Technical Field
The invention relates to micro-flow control, in particular to a detection device based on micro-flow control technology and a working method thereof.
Background
Microfluidic technology refers to the science and technology involved in systems that process or manipulate microfluidics using tens to hundreds of microns of tubing, an emerging interdiscipline involving chemical, fluid physics, microelectronics, new materials, biology and biomedical engineering.
The centrifugal microfluidic system is a microfluidic system which integrates valves, flow pipelines, mixing reactors, sample adding, separating, detecting and other components involved in the processes of sampling, pretreatment, derivatization, mixing, detecting and the like of chemical analysis on a CD-shaped disc by taking a micro-electromechanical technology as a basis, and realizes detection and analysis of liquid flow by taking centrifugal force as a driving force of the liquid flow. By utilizing the principle of centrifugal speed control, the operation flow of the experiment can be controlled easily by a program, and the whole experiment flow can be completed by only adding a reagent and running the program for a detector.
Chemiluminescent immunoassay (chemiluminescence immunoassay, CLIA) is a detection assay technique for various antigens, haptens, antibodies, hormones, enzymes, drugs, etc., by combining a chemiluminescent assay technique with high sensitivity with a highly specific immune reaction. In order to improve the dispersion of antigen-antibody coating, magnetic beads are generally used as carriers, the magnetic beads are injected into a reaction tank to react for coating, and the release of the magnetic beads is controlled by a magnet to realize separation.
Chemiluminescent immunoassay comprises two components, namely an immunoreaction system and a chemiluminescent assay system. The chemiluminescent system is to utilize the catalysis of a chemiluminescent substance through a catalyst and the oxidation of an oxidant to form an intermediate in an excited state, and emit photons when the intermediate in the excited state returns to a stable ground state, and measure the light quantum yield by utilizing a luminescent signal analyzer. The immune reaction system is to directly label the luminescent substance on the antigen or antibody. Acting as an enzyme on a luminescent substrate.
The micro-fluidic centrifugal magnetic particle manipulation can solve the magnetic bead manipulation problem in the chemiluminescence projects, and the like, and is a good manner for the middle cleaning link in a mixed vibration manner, so that the cleaning is more thorough.
The patent application No. cn201410669355.X provides a centrifugal magnetic particle manipulation and detection device and its operation method, in which the operation mode is that the magnet attracts the magnetic beads into a group and periodically moves in the solution to complete the steps of antigen-antibody binding, cleaning and substrate color development. The main disadvantages of this scheme are:
the magnet always attracts the magnetic beads into a group, so that the dispersibility is lost; in the washing and substrate reaction stage, the magnetic beads are driven to move by the movement of the upper magnet and the lower magnet, so that the mixing is realized, the mixing effect is poor, the efficiency is low, and the time is long. More importantly, in the cleaning process, poor dispersion can cause uneven cleaning effect, residual reactants are not cleaned, and the result is distorted.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides the detection device based on the microfluidic technology, which has high sensitivity and short detection time.
The invention aims at realizing the following technical scheme:
a detection device based on a microfluidic technology, which comprises a first disc, wherein the first disc is provided with a plurality of detection units, and the detection units comprise a detection tank and a waste liquid tank; the microfluidic technology-based detection device further comprises:
a second disk disposed below the first disk; the second disc is provided with a plurality of magnetic units, the number of the magnetic units is equal to that of the detection units, and the magnetic units comprise limiting grooves and first magnetic parts; the included angle between the trend of the limit groove and the radial direction of the second disc is an acute angle, and the first magnetic force piece is arranged in the limit groove;
the second magnetic force piece is fixed on the outer side of the limiting groove, which is close to the center of the second disc; the second magnetic member remains relatively stationary with the second disk.
The invention also aims to provide a working method of the microfluidic technology-based detection device with high detection sensitivity and short detection time, which is realized by the following technical scheme:
according to the working method of the detection device based on the microfluidic technology, the working method comprises the following steps:
the initial stage: under the attraction of the second magnetic force piece, the first magnetic force piece is positioned in the limiting groove and is far away from the detection groove on the first disk at the position of the end part of the center of the circle of the second disk;
incubation: the first disc and the second disc enter a low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece at the position of the end part, which is close to the center of the circle, in the limit groove;
the first disc is rotated positively and negatively, the dispersed magnetic beads move back and forth in the limit groove, and the sample, the magnetic bead antibody and the enzyme-immune antibody are mixed and incubated;
and (3) cleaning: the cleaning liquid enters the detection groove, the first disc and the second disc enter a low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece at the position of the end part, which is close to the circle center, in the limit groove;
the first disc is rotated in the forward and reverse directions, and the dispersed magnetic beads move back and forth in the detection groove and are cleaned by the cleaning liquid;
waste discharge stage: the first disc and the second disc enter a high-speed mode, and the first magnetic force piece is thrown to the position of the end part, far away from the center of the circle, in the limit groove, so that magnetic beads in the detection groove are adsorbed;
liquid in the detection tank enters the waste liquid tank;
reaction stage: the substrate enters a detection groove, a first disc and a second disc enter a low-speed mode, and a first magnetic member is adsorbed by a second magnetic member at the position, close to the end part of the circle center, in the limit groove;
the first disk is rotated in forward and reverse directions, and the dispersed magnetic beads move back and forth in the detection groove and react with the substrate.
Compared with the prior art, the invention has the following beneficial effects:
1. under the action of centrifugal force and magnetic attraction force, the magnets in different time periods are realized at different positions, and the dispersion and aggregation of the magnetic beads are realized;
the magnetic beads are dispersed in high luminous intensity and short process time. Due to the sufficient mixing, corresponding time can be shortened in the incubation stage, the cleaning stage and the substrate reaction stage, namely, the detection time is shortened, and the light intensity value under the same system can be enhanced due to high antigen-antibody binding efficiency after dispersion, so that the sensitivity is improved;
2. in the incubation stage, the cleaning stage and the substrate reaction stage, the motor drives the disc to rotate forwards and reversely to disperse the magnetic beads (the magnet is far away from the magnetic beads), so that the homogeneous reaction is realized.
Drawings
The present disclosure will become more readily understood with reference to the accompanying drawings. As will be readily appreciated by those skilled in the art: the drawings are only for illustrating the technical scheme of the present invention and are not intended to limit the scope of the present invention. In the figure:
fig. 1 is a schematic diagram of the structure of a second disc according to embodiment 1 of the present invention.
Detailed Description
Fig. 1 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. In order to teach the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or alternatives derived from these embodiments that fall within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the invention is not limited to the following alternative embodiments, but only by the claims and their equivalents.
Example 1:
the detection device based on the micro-fluidic technology provided by the embodiment of the invention has a simple structure, and comprises:
the first disc is arranged above the second disc, and the second disc is provided with a plurality of detection units, wherein the detection units comprise detection grooves and waste liquid grooves; the structure and function of the first disc is prior art, see in particular the patent application of cn201410669355. X;
fig. 1 schematically shows a schematic structure of a second disc according to an embodiment of the present invention, as shown in fig. 1, the second disc including:
the magnetic units are equal to the detection units in number and comprise limiting grooves 2 and first magnetic members 4; the included angle between the trend of the limit groove and the radial direction of the second disc is an acute angle, and the first magnetic force piece is arranged in the limit groove;
and the second magnetic member 3 is fixed on the outer side of the limiting groove, which is close to the center of the second disc, such as the outer side of the end part of the limiting groove, which is close to the center of the center.
The working method of the detection device based on the microfluidic technology comprises the following steps:
the initial stage: under the attraction of the second magnetic force piece, the first magnetic force piece is positioned in the limiting groove and is far away from the detection groove on the first disk at the position of the end part of the center of the circle of the second disk;
incubation: the first disc and the second disc enter a low-speed mode, the first magnetic member is absorbed by the second magnetic member at the position of the end part of the limiting groove, which is close to the center of the circle, namely attractive force between the first magnetic member and the second magnetic member provides centripetal force required by rotation of the first magnetic member;
the first disc is rotated positively and negatively, the dispersed magnetic beads move back and forth in the limit groove, and the sample, the magnetic bead antibody and the enzyme-immune antibody are mixed and incubated;
and (3) cleaning: the cleaning liquid enters the detection groove, the first disc and the second disc enter a low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece at the position of the end part, which is close to the circle center, in the limit groove;
the first disc is rotated in the forward and reverse directions, and the dispersed magnetic beads move back and forth in the detection groove and are cleaned by the cleaning liquid;
waste discharge stage: the first disc and the second disc enter a high-speed mode, attractive force between the first magnetic force piece and the second magnetic force piece is insufficient to provide centripetal force required by high-speed rotation of the first magnetic force piece, and the first magnetic force piece is thrown to a position in the limit groove, which is far away from the end part of the circle center, so that magnetic beads in the detection groove are adsorbed;
liquid in the detection tank enters the waste liquid tank;
reaction stage: the substrate enters a detection groove, a first disc and a second disc enter a low-speed mode, and a first magnetic member is adsorbed by a second magnetic member at the position, close to the end part of the circle center, in the limit groove;
the first disk is rotated in forward and reverse directions, and the dispersed magnetic beads move back and forth in the detection groove and react with the substrate.
It can be seen that the second magnetic member attracts the first magnetic member throughout the incubation, washing and reaction phases described above, such that the first magnetic member and the second magnetic member remain relatively stationary.
Example 2:
according to the application example of the detection device and the working method based on the micro-fluidic technology in the embodiment 1 of the invention.
In the application example, the first disc is provided with 6 detection units, correspondingly, the second disc is provided with 6 limit grooves, the limit grooves are linear, and two ends of the limit grooves are semicircular; the first magnetic parts adopt permanent magnets, the second magnetic parts are 6, are respectively fixed on the second disc and are arc-shaped, are matched with the end parts of the limiting grooves in shape, and adopt permanent magnets; the rotation radius of the second magnetic member under driving is the same, and the second magnetic member and the limit groove (namely the second disc) are kept relatively static.
In the working method of the detection device, after the cleaning liquid is added in the cleaning stage, the rotating second disc suddenly stops, and the first magnetic member slides to a position in the limiting groove, which is close to the end part of the circle center, and is attracted by the second magnetic member.
Example 3:
according to the application example of the detection device and the working method based on the micro-fluidic technology in the embodiment 1 of the invention.
In the application example, the first disc is provided with 6 detection units, correspondingly, the second disc is provided with 6 limit grooves, and the limit grooves are in a straight line shape; the first magnetic force piece adopts a permanent magnet, and 6 second magnetic force pieces are connected together to form a ring shape and adopt the permanent magnet; the detection device also comprises a third disc, the third disc is arranged between the first disc and the second disc, the second magnetic force piece is fixed on the third disc, and the first disc, the second disc and the third disc rotate together under the drive of a motor, namely the first disc, the second disc and the third disc are kept relatively static.
In the working method of the detection device, after the cleaning liquid is added in the cleaning stage, the rotating second disc suddenly stops, and the first magnetic member slides to a position in the limiting groove, which is close to the end part of the circle center, and is attracted by the second magnetic member.
The above embodiments are given by way of example only in the case of the second magnetic member being a permanent magnet, although ferromagnetic materials may also be used for the second magnetic member.
Claims (6)
1. According to the working method of the detection device based on the micro-fluidic technology, the detection device based on the micro-fluidic technology comprises a first disc, wherein the first disc is provided with a plurality of detection units, and the detection units comprise a detection groove and a waste liquid groove; the detection device based on the micro-fluidic technology further comprises a second disc arranged below the first disc; the second disc is provided with a plurality of magnetic units, the number of the magnetic units is equal to that of the detection units, and the magnetic units comprise limiting grooves and first magnetic parts; the included angle between the trend of the limit groove and the radial direction of the second disc is an acute angle, and the first magnetic force piece is arranged in the limit groove; the second magnetic member is fixed on the outer side of the end part of the limiting groove, which is close to the center of the circle of the second disc; the second magnetic member is arc-shaped and matched with the end part in shape; the working method comprises the following steps:
the initial stage: under the attraction of the second magnetic force piece, the first magnetic force piece is positioned in the limiting groove and is far away from the detection groove on the first disk at the position of the end part of the center of the circle of the second disk;
incubation: the first disc and the second disc enter a low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece at the position of the end part, which is close to the center of the circle, in the limit groove;
the first disc is in forward and reverse rotation, the dispersed magnetic beads move back and forth in the detection groove, and the sample, the magnetic bead antibody and the enzyme-immune antibody are mixed and incubated;
and (3) cleaning: the cleaning liquid enters the detection groove, the first disc and the second disc enter a low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece at the position of the end part, which is close to the circle center, in the limit groove;
the first disc is rotated in the forward and reverse directions, and the dispersed magnetic beads move back and forth in the detection groove and are cleaned by the cleaning liquid;
waste discharge stage: the first disc and the second disc enter a high-speed mode, and the first magnetic force piece is thrown to the position of the end part, far away from the center of the circle, in the limit groove, so that magnetic beads in the detection groove are adsorbed;
liquid in the detection tank enters the waste liquid tank;
reaction stage: the substrate enters a detection groove, a first disc and a second disc enter a low-speed mode, and a first magnetic member is adsorbed by a second magnetic member at the position, close to the end part of the circle center, in the limit groove;
the first disc is in forward and reverse rotation, and the dispersed magnetic beads move back and forth in the detection groove and react with the substrate;
the second magnetic member attracts the first magnetic member throughout the incubation period, the washing period, and the reaction period such that the first magnetic member and the second magnetic member remain relatively stationary.
2. The method of operation of claim 1, wherein: in the cleaning stage, after the cleaning liquid is added, the rotating second disc suddenly stops, and the first magnetic member slides to a position in the limiting groove, which is close to the center of the circle, and is attracted by the second magnetic member.
3. The method of operation of claim 1, wherein: the second magnetic force piece has the same rotation radius under the driving.
4. The method of operation of claim 1, wherein: the second magnetic force piece is combined into a ring shape.
5. The method of operation of claim 4, wherein: the detection device further includes:
and the second magnetic part is fixed on the third disc.
6. The method of operation of claim 1, wherein: the first magnetic force piece adopts a magnet, and the second magnetic force piece adopts a magnet or ferromagnetic material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710585320.1A CN107552115B (en) | 2017-07-18 | 2017-07-18 | Detection device based on microfluidic technology and working method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710585320.1A CN107552115B (en) | 2017-07-18 | 2017-07-18 | Detection device based on microfluidic technology and working method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107552115A CN107552115A (en) | 2018-01-09 |
| CN107552115B true CN107552115B (en) | 2023-06-23 |
Family
ID=60973636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710585320.1A Active CN107552115B (en) | 2017-07-18 | 2017-07-18 | Detection device based on microfluidic technology and working method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107552115B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925925B (en) * | 2020-08-17 | 2024-02-27 | 鄂州康芯医疗科技有限公司 | Integrated diagnostic kit and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201516410A (en) * | 2013-10-31 | 2015-05-01 | Univ Feng Chia | Microfluidic disc analyzer |
| CN105675857A (en) * | 2014-10-31 | 2016-06-15 | 绍兴普施康生物科技有限公司 | Centrifugal magnetic particle control and detection device and operation method thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7763453B2 (en) * | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
| EP1939629A3 (en) * | 2006-08-11 | 2011-03-09 | Samsung Electronics Co., Ltd. | Centrifugal Force Based Magnet Position Control Device and Disk-Shaped Micro Fluidic System |
| CN101918528B (en) * | 2007-12-10 | 2013-07-17 | 三星电子株式会社 | A thin film bio valve device and its controlling apparatus |
| KR20130080307A (en) * | 2012-01-04 | 2013-07-12 | 삼성전자주식회사 | Microfluidic apparatus comprising rotatable disc-type body, and method for separating target material and amplifying nucleic acid using the same |
| DE102012205523A1 (en) * | 2012-04-04 | 2013-10-10 | Robert Bosch Gmbh | Reagent vessel insert and reagent vessel |
| CN103852577A (en) * | 2012-11-28 | 2014-06-11 | 三星电子株式会社 | Microfluidic apparatus and method of enriching target cell |
| TWI477321B (en) * | 2012-12-28 | 2015-03-21 | Ind Tech Res Inst | Micro flow mixing apparatus and method thereof |
| CN104597266B (en) * | 2013-10-31 | 2017-04-12 | 逢甲大学 | Centrifugal-type detection platform and operating process thereof |
| TWI562829B (en) * | 2015-06-17 | 2016-12-21 | Delta Electronics Inc | Centrifugal channel device and centrifugal channel main body |
| CN206965757U (en) * | 2017-07-18 | 2018-02-06 | 绍兴普施康生物科技有限公司 | Detection means based on microflow control technique |
-
2017
- 2017-07-18 CN CN201710585320.1A patent/CN107552115B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201516410A (en) * | 2013-10-31 | 2015-05-01 | Univ Feng Chia | Microfluidic disc analyzer |
| CN105675857A (en) * | 2014-10-31 | 2016-06-15 | 绍兴普施康生物科技有限公司 | Centrifugal magnetic particle control and detection device and operation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107552115A (en) | 2018-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hayes et al. | Flow-based microimmunoassay | |
| CN103384565B (en) | Method of transporting magnetic particles | |
| US7892856B2 (en) | Flow-controlled magnetic particle manipulation | |
| JP5322996B2 (en) | Centrifugal microfluidic system and method for automated sample analysis | |
| TWI545323B (en) | Centrifugal magnetic bead operating apparatus and operating method thereof | |
| CN107044972A (en) | A kind of micro-fluidic chip fluorescence immunoassay quick detection kit and its preparation and detection method | |
| KR20140060263A (en) | Microfluidic disc for use in with bead-based immunoassays | |
| US20070113908A1 (en) | Valve for microfluidic chips | |
| CN109718877B (en) | Centrifugal disc type micro-fluidic chip and using method thereof | |
| JP2007101318A (en) | Analyzer | |
| CN107552115B (en) | Detection device based on microfluidic technology and working method thereof | |
| Kinahan et al. | Automation of silica bead-based nucleic acid extraction on a centrifugal lab-on-a-disc platform | |
| Chang et al. | A microchannel immunoassay chip with ferrofluid actuation to enhance the biochemical reaction | |
| CN113967486A (en) | Centrifugal micro-fluidic chip | |
| Min et al. | An automated microfluidic chemiluminescence immunoassay platform for quantitative detection of biomarkers | |
| CN103223323B (en) | Magnetic separation technology and micro-fluid technology based rapid detection micro-fluid reactor, and making method and detection method thereof | |
| CN211528425U (en) | Immune magnetic bead chemiluminescence analysis system based on micro-fluidic technology | |
| JPH10300752A (en) | Sample analyzing device | |
| CN206965757U (en) | Detection means based on microflow control technique | |
| CN111007243A (en) | Magnetic field switching rotating member, magnetic field switching device, optical detection device, and heat preservation device | |
| CN211528426U (en) | Immune magnetic bead chemiluminescence analysis system based on micro-fluidic technology | |
| CN104697841B (en) | Magnetic particle separation and transfer device, method and application thereof | |
| JP2006218442A (en) | Apparatus and method for b/f separation in immunoassay | |
| CN110794133A (en) | Immune magnetic bead chemiluminescence analysis system based on microfluidic technology and working method | |
| Carthy et al. | Automated solid phase DNA extraction on a lab-on-a-disc with two-degrees of freedom instrumentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: Room 408, building C, scientific research building, No. 398, mahuan Road, Lihai Town, Binhai New City, Shaoxing City, Zhejiang Province Applicant after: Zhejiang pushkang Biotechnology Co.,Ltd. Address before: 312073 Zhejiang city in Shaoxing Province, the coastal town of Hai Zhen Ma Huanlu No. 398 research building C Building Room 408 Applicant before: SHAOXING PUSHIKANG BIOTECHNOLOGY CO.,LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |