CN211528425U - Immune magnetic bead chemiluminescence analysis system based on micro-fluidic technology - Google Patents
Immune magnetic bead chemiluminescence analysis system based on micro-fluidic technology Download PDFInfo
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- CN211528425U CN211528425U CN201921760773.4U CN201921760773U CN211528425U CN 211528425 U CN211528425 U CN 211528425U CN 201921760773 U CN201921760773 U CN 201921760773U CN 211528425 U CN211528425 U CN 211528425U
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Abstract
The utility model provides an immunomagnetic bead chemiluminescence analysis system based on microfluidic technology, the analysis system comprises a microfluidic disc with a plurality of detection cavities, and a control device comprises a first bearing part and a plurality of electromagnets which are circumferentially distributed along the center of the first bearing part; the switch is connected with an external power supply and the electromagnet through a lead; the control device is fixedly arranged; in the vertical direction, the control device and the microfluidic disc are sequentially arranged from top to bottom or from bottom to top. The utility model has the advantages of simple structure, convenient operation, high analysis efficiency and the like.
Description
Technical Field
The utility model relates to a micro-fluidic, in particular to immunomagnetic bead chemiluminescence analytic system based on micro-fluidic technique.
Background
Microfluidic technology refers to the science and technology involved in systems that process or manipulate microfluidics using tens to hundreds of microns of tubing, and is an emerging interdiscipline that involves chemical, fluid physical, microelectronic, new materials, biological, and biomedical engineering.
The centrifugal micro-fluidic system is a micro-fluidic system which is based on micro-electro-mechanical technology, integrates valves, flow pipelines, mixing reactors, sample adding, separating, detecting and other parts involved in the processes of sampling, preprocessing, derivation, mixing, detecting and the like of chemical analysis on a CD-shaped disc, and realizes the detection and analysis of liquid flow by taking centrifugal force as the driving force of the liquid flow. By utilizing the principle of centrifugal speed control, the operation process of the experiment can be easily controlled by a program, and the whole experiment process can be completed by adding a reagent and running the program for detection personnel to obtain an experiment result.
Chemiluminescence immunoassay (CLIA) is a detection and analysis technique for various antigens, haptens, antibodies, hormones, enzymes, drugs and the like by combining a chemiluminescence assay technique with high sensitivity and a high specificity immunoreaction. In order to improve the loss of the antigen-antibody coating, magnetic beads are generally used as carriers, the magnetic beads are injected into a reaction tank for reaction and coating, and the release of the magnetic beads is controlled by a magnet to realize the separation.
Chemiluminescent immunoassays comprise two components, an immunoreaction system and a chemiluminescent assay system. The chemiluminescence system is characterized in that chemiluminescence substances are catalyzed by a catalyst and oxidized by an oxidant to form an excited intermediate, the excited intermediate emits photons when returning to a stable ground state, and the yield of light quanta is measured by a luminescence signal determinator. The immune reaction system is to label the luminous substance directly on the antigen or antibody. The enzyme is used on a luminescent substrate.
The manipulation of the microfluidic centrifugal magnetic particles can solve the manipulation problem of magnetic beads in the items such as chemiluminescence and the like, and the cleaning link is well cleaned in a mixed oscillation mode, so that the cleaning is more thorough.
The current conventional way is: the magnetic beads are only controlled to be cleaned by a magnet below the microfluidic disc, and the scheme has the main defects that:
magnet can only adsorb the below lower surface that detects the chamber with the magnetic bead, when just reversing and vibrate the mixing magnetic bead, the magnetic bead that the bottom was held group is difficult to be shaken, leads to wasing not thoroughly, can cause abluent effect deviation to have remaining reactant not wash, causes the result distortion, simultaneously also not thoroughly when reacting with the substrate.
SUMMERY OF THE UTILITY MODEL
For solving not enough among the above-mentioned prior art scheme, the utility model provides an accurate immunomagnetic bead chemiluminescence analytic system based on micro-fluidic technology of analysis.
The utility model aims at realizing through the following technical scheme:
an immunomagnetic bead chemiluminescence analysis system based on microfluidic technology comprises a microfluidic disc, wherein the microfluidic disc is provided with a plurality of detection cavities; the analysis system further comprises:
the control device comprises a first bearing piece and a plurality of electromagnets which are distributed along the center of the first bearing piece in the circumferential direction;
the switch is connected with an external power supply and the electromagnet through a lead;
the control device is fixedly arranged; in the vertical direction, the control device and the microfluidic disc are sequentially arranged from top to bottom or from bottom to top.
Compared with the prior art, the utility model discloses the beneficial effect who has does:
1. the analysis is accurate;
only the control device is arranged above or below the microfluidic disc piece independently, so that the electromagnet adsorbs the magnetic beads in the detection cavity as required, and the control devices are not required to be arranged above or below the detection cavity; in the cleaning stage, the magnetic beads are cleaned more sufficiently and thoroughly, and in the reaction stage, the magnetic beads and the substrate react sufficiently, so that the accuracy of subsequent analysis is improved;
2. the static electromagnet is accurately and quickly controlled, the magnetic bead control is easier to realize, no moving part is provided, and the magnetic bead loss phenomenon is avoided;
3. through the design of the extension parts, the microfluidic disc is stably clamped between the extension parts, the microfluidic disc and the second bearing part rotate synchronously, the situation that the detection result is influenced due to inaccurate positioning of the detection cavity caused by relative movement of the second bearing part and the microfluidic disc is prevented, and the accuracy of the detection result is ensured.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only intended to illustrate the technical solution of the present invention and are not intended to limit the scope of the present invention. In the figure:
fig. 1 is a schematic diagram of an immunomagnetic bead chemiluminescence analysis system based on microfluidic technology according to an embodiment of the present invention;
fig. 2 is a schematic view of a second carrier according to an embodiment of the present invention.
Detailed Description
Fig. 1-2 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. For the purpose of teaching the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or substitutions from these embodiments that will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Accordingly, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
fig. 1 schematically shows a schematic structural diagram of an immunomagnetic bead chemiluminescence analysis system of the microfluidic technology according to embodiment 1 of the present invention, and as shown in fig. 1, the immunomagnetic bead chemiluminescence analysis system of the microfluidic technology includes:
a microfluidic disc 21 having a plurality of detection chambers; the micro-fluidic disc is the prior art in the field, and the specific structure and the working mode are not described again;
the control device 10 comprises a first bearing piece 11 and a plurality of electromagnets 12 distributed along the center of the first bearing piece in the circumferential direction;
the switch 13 is connected with an external power supply and the electromagnet 12 through a lead;
the control device 10 is fixedly arranged, and in the vertical direction, the control device 10 and the microfluidic disk 21 are sequentially arranged from top to bottom or from bottom to top, preferably from top to bottom.
In order to improve the influence of the electromagnets on the magnetic beads in the detection chambers and improve the degree of enrichment of subsequent washing and reaction, further, the projections of the detection chambers on the horizontal plane and the projections of the electromagnets on the horizontal plane have overlap, and the number of the electromagnets is equal to that of the detection chambers.
In order to control the magnetic beads in the detection cavity to move between the upper wall and the lower wall of the detection cavity by using the electromagnet and improve the sufficiency of cleaning and reaction, further, the distance from the center of the electromagnet to the center of the first bearing piece is equal to the rotating radius of the center of the detection cavity.
In order to conveniently and firmly fix the microfluidic disc, the analysis system further comprises:
and the second bearing member 36, on which the microfluidic disk 21 is arranged, is kept relatively stationary with the second bearing member 36, that is, the microfluidic disk rotates synchronously with the second bearing member.
In order to ensure that the second control device and the microfluidic disk rotate synchronously to achieve relative standstill, the outer edge of the second carrier 36 further has at least two extensions 34 extending in a direction parallel to the central axis of the second carrier, and the microfluidic disk 21 is clamped in the area enclosed by the extensions.
In order to prevent the relative movement between the microfluidic disc 21 and the second control device 30, further, the edge of the microfluidic disc 21 has a notch, and the side wall of the extension 34 opposite to the microfluidic disc 21 has a protrusion 35 adapted to be snapped into the notch.
The above-mentioned immunomagnetic bead chemiluminescence analytic system based on the microfluidic technology has the working mode:
an initial stage: the electromagnet is powered off, and a sample, enzyme label and magnetic bead are added into the detection cavity;
an incubation stage: the electromagnet is still in a power-off state, and the microfluidic disc rotates forwards and backwards to oscillate for incubation;
a waste discharge stage: electrifying the electromagnet, adsorbing the magnetic beads on the upper surface of the detection cavity, allowing the cleaning solution to enter the detection cavity, operating the microfluidic disc at a high speed, and allowing the waste liquid to enter a waste liquid tank;
a cleaning stage: cleaning liquid enters the detection cavity, the electromagnet is in a power-off state, the microfluidic disc rotates forwards and backwards, dispersed magnetic beads move back and forth in the detection cavity, and the magnetic beads are cleaned by the cleaning liquid;
a waste discharge stage: electrifying the electromagnet, adsorbing the magnetic beads on the upper surface of the detection cavity, allowing the cleaning solution to enter the detection cavity, operating the microfluidic disc at a high speed, and allowing the liquid in the detection cavity to enter a waste liquid tank;
a reaction stage: the substrate enters the detection cavity, the electromagnet is in a power-off state, the microfluidic disc rotates forwards and backwards, the dispersed magnetic beads move back and forth in the detection cavity, and the magnetic beads are in full contact reaction with the substrate.
Example 2:
according to the utility model discloses embodiment 1's immunomagnetic bead chemiluminescence analytic system's based on micro-fluidic technology application example.
In this application example, as shown in fig. 1-2, the control device 10 and the microfluidic disk 21 are arranged in this order from top to bottom in the vertical direction; the first bearing piece 11 is in a disc structure, grooves are uniformly arranged on a concentric circle taking the center of the first bearing piece 11 as the center of a circle, and the electromagnets 12 are arranged in the grooves; the electromagnets 12 are in a parallel structure, and the electrification of all the electromagnets can be controlled by only one switch 13; the second bearing member 36 is a disc structure, the outer edge of the second bearing member has at least two extending portions 34 extending along a direction parallel to the central axis of the second bearing member, the side wall of the extending portion 34 opposite to the microfluidic disk 21 has a cylindrical protrusion 35, correspondingly, the edge of the microfluidic disk 21 has a cylindrical notch for 35 to be inserted, so that the microfluidic disk 21 is relatively and statically inserted in the area enclosed by the extending portions 34; the connecting line of the center of the first bearing part 11 and the rotation center of the second bearing part 36 is perpendicular to the rotation surface of the microfluidic disc 21; the projection of the detection cavity on the horizontal plane is overlapped with the projection of the electromagnet on the horizontal plane, so that the electromagnet is arranged right above each detection cavity after the microfluidic disc rotates; the distance from the center of the electromagnet to the center of the first bearing piece is equal to the rotation radius of the center of the detection cavity.
Claims (8)
1. An immunomagnetic bead chemiluminescence analysis system based on microfluidic technology comprises a microfluidic disc, wherein the microfluidic disc is provided with a plurality of detection cavities; the method is characterized in that: the analysis system further comprises:
the control device comprises a first bearing piece and a plurality of electromagnets which are distributed along the center of the first bearing piece in the circumferential direction;
the switch is connected with an external power supply and the electromagnet through a lead;
the control device is fixedly arranged; in the vertical direction, the control device and the microfluidic disc are sequentially arranged from top to bottom or from bottom to top.
2. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 1, wherein: the projection of the detection cavities on the horizontal plane and the projection of the electromagnets on the horizontal plane are overlapped, and the number of the detection cavities is equal to that of the electromagnets.
3. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 1, wherein: the first bearing piece is provided with a groove, and the electromagnet is arranged in the groove.
4. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 1, wherein: the distance from the center of the electromagnet to the center of the first bearing piece is equal to the rotation radius of the center of the detection cavity.
5. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 1, wherein: the analysis system further comprises:
a second carrier on which the microfluidic disk is disposed, the second carrier remaining relatively stationary.
6. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 5, wherein: the outer edge of the second bearing part is provided with at least two extending parts extending along the direction parallel to the central axis of the second bearing part, and the microfluidic disc is clamped in the area enclosed by the extending parts.
7. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 6, wherein: the edge of the microfluidic disc is provided with a notch, and the side wall of the extension part opposite to the microfluidic disc is provided with a protrusion suitable for being clamped into the notch.
8. The microfluidic technology-based immunomagnetic bead chemiluminescence analysis system of claim 7, wherein: the notch and the bulge are columnar.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921760773.4U CN211528425U (en) | 2019-10-21 | 2019-10-21 | Immune magnetic bead chemiluminescence analysis system based on micro-fluidic technology |
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| CN201921760773.4U CN211528425U (en) | 2019-10-21 | 2019-10-21 | Immune magnetic bead chemiluminescence analysis system based on micro-fluidic technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112462048A (en) * | 2021-02-02 | 2021-03-09 | 南昌大学 | Microfluidic detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112462048A (en) * | 2021-02-02 | 2021-03-09 | 南昌大学 | Microfluidic detection device |
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