CN107550949A - The preparation method of high bioavilability Huperzine Serrate P.E - Google Patents
The preparation method of high bioavilability Huperzine Serrate P.E Download PDFInfo
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- CN107550949A CN107550949A CN201710977045.8A CN201710977045A CN107550949A CN 107550949 A CN107550949 A CN 107550949A CN 201710977045 A CN201710977045 A CN 201710977045A CN 107550949 A CN107550949 A CN 107550949A
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Abstract
The invention discloses the preparation method of high bioavilability Huperzine Serrate P.E, comprise the following steps：Mixed according to a certain percentage after serrate clubmoss herb is crushed with licorice raw material, extracted with water at atmospheric pressure, be filtrated to get extract solution；Extract solution be concentrated under reduced pressure into it is a certain amount of, and stand filtering after concentrate obtain filtrate；Gained filtrate is extracted with a certain amount of water-saturated n-butanol, extracting n-butyl alcohol layer is recovered under reduced pressure solvent and obtains medicinal extract, extract dry obtains high bioavilability Huperzine Serrate P.E after crushing, the present invention is used as raw material using serrate clubmoss herb and radix glycyrrhizae, Huperzine Serrate P.E is obtained out by filtering and extracting the step of refiltering, its step is simple, when consumption it is few, suitable for putting into industrialized production, Huperzine Serrate P.E of the present invention can be configured to the various preparations in pharmacy as active component and pharmaceutical carrier, said preparation includes oral, injection and local administration, it can be used for preparing and improve memory, prevent and treat the medicine of senile dementia.
Description
Technical field
The present invention relates to Huperzine Serrate P.E preparation method technical field, specially high bioavilability Huperzine Serrate P.E
Preparation method.
Background technology
Serrate clubmoss herb, also known as Huperzia serrata, short Chinese fir, be Huperziaceae plants of Huperzia, be mainly distributed on the Changjiang river in China with
The height above sea level such as south, northeast, northwest and Taiwan are 1500~3000 meters of area.Its active ingredient is that huperzine is serrate clubmoss herb extraction
Thing, it is efficient anticholinesterase, pharmacological evaluation proves that huperzine can improve study, memory effect, and has been used for
Clinical treatment myasthenia gravis, effective percentage also have certain curative effect up to 99%, and to senile amnesia and senile dementia.
Because the huperzine obtained from natural resources can not meet clinical needs, in the past few years many scientists develop and carried
The method for taking Huperzine Serrate P.E, such as a kind of Huperzine Serrate P.E of Publication No. CN101396380A propositions and its preparation side
Method, the methods of it is ground using serrate clubmoss herb medicinal material, Diluted Alcohol refluxing extraction, obtain Huperzine Serrate P.E, its extraction time
Long, step is complicated.
The content of the invention
It is an object of the invention to provide the preparation method of high bioavilability Huperzine Serrate P.E, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the above object, the present invention provides following technical scheme：The preparation of high bioavilability Huperzine Serrate P.E
Method, comprise the following steps：
(1) mixed according to a certain percentage after serrate clubmoss herb is crushed with licorice raw material, extracted with water at atmospheric pressure, be filtrated to get extraction
Liquid；
(2) extract solution is concentrated under reduced pressure into a certain amount of, and the concentrate stood after filtering obtains filtrate；
(3) gained filtrate is extracted with a certain amount of water-saturated n-butanol, extracting n-butyl alcohol layer is recovered under reduced pressure solvent and obtained
Medicinal extract, extract dry obtain high bioavilability Huperzine Serrate P.E after crushing.
Preferably, the mixed proportion of serrate clubmoss herb and radix glycyrrhizae coarse powder is in the step (1):1：(1—3).
Preferably, extracting method is in the step (1)：Extracted 2 times with 85-100 DEG C of water, it is thousand to add water for the first time
6-10 times of layer tower and radix glycyrrhizae compound weight, extraction time be 1-2h, add water and mixed for serrate clubmoss herb with radix glycyrrhizae for second
Close material 8-12 times, extraction time be 0.5-1.5h.
Preferably, in the step (2), extract solution concentration amount is 0.5-1 times of raw material total amount in step (1) and measured, and filters
For method to be filtered using bag filter, the filter bag of bag filter filtering is 200-400 mesh filter bags.
Preferably, in the step (3), water-saturated n-butanol dosage used is 1-3 times of gained filtrate in step (2)
Amount, extraction times are 1-5 times.
Preferably, in the step (3), between drying temperature is 40-70 DEG C.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention passed through using serrate clubmoss herb and radix glycyrrhizae as raw material
Filter and extraction the step of refiltering, obtain out Huperzine Serrate P.E, and its step is simple, when consumption it is few, suitable for putting into industrial metaplasia
Production, Huperzine Serrate P.E of the present invention can be configured to the various preparations in pharmacy, said preparation as active component and pharmaceutical carrier
Including oral, injection and part administration, can be used for preparing the medicine for improving memory, prevention and treatment senile dementia.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example only part of the embodiment of the present invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
Embodiment one：
Mix after 15kg serrate clubmoss herbs are crushed with 15kg licorice raw materials, extracted 2 times with 85 DEG C of water at atmospheric pressure, add water for the first time
6 times, extraction time 1h for serrate clubmoss herb and radix glycyrrhizae compound weight are measured, it is that serrate clubmoss herb mixes with radix glycyrrhizae that second, which is added water,
8 times, extraction time 0.5h of material, are filtrated to get extract solution；
Extract solution is concentrated under reduced pressure into 0.5 times of raw material total amount, and uses bag filter mistake of the filter bag for 200 mesh filter bags
Filter, stand the concentrate after filtering and obtain filtrate；
Water-saturated n-butanol extraction time by gained filtrate with 1 times of amount of filtrate, solvent is recovered under reduced pressure in extracting n-butyl alcohol layer
Medicinal extract is obtained, medicinal extract obtains high bioavilability Huperzine Serrate P.E 1.3kg after being crushed at being 40 DEG C in drying temperature.
Embodiment two：
Mix after 7kg serrate clubmoss herbs are crushed with 21kg licorice raw materials, extracted 2 times with 100 DEG C of water at atmospheric pressure, add water for the first time
10 times, extraction time 2h for serrate clubmoss herb and radix glycyrrhizae compound weight are measured, it is that serrate clubmoss herb mixes with radix glycyrrhizae that second, which is added water,
12 times, extraction time 1.5h of material are closed, is filtrated to get extract solution；
Extract solution is concentrated under reduced pressure into 1 times of raw material total amount, and uses filter bag to be filtered for the bag filter of 400 mesh filter bags,
Stand the concentrate after filtering and obtain filtrate；
Gained filtrate is extracted 5 times with the water-saturated n-butanol of 3 times of amounts of filtrate, solvent is recovered under reduced pressure in extracting n-butyl alcohol layer
Medicinal extract is obtained, medicinal extract obtains high bioavilability Huperzine Serrate P.E 1.2kg after being crushed at being 70 DEG C in drying temperature.
Embodiment three：
According to 1 after 10kg serrate clubmoss herbs and 20kg licorice raw materials are crushed：2 ratios mix, and are extracted 2 times with 93 DEG C of water at atmospheric pressure,
Addition water is 8 times, extraction time 1.5h of serrate clubmoss herb and radix glycyrrhizae compound weight for the first time, and it is thousand that second, which is added water,
10 times, extraction time 1h of layer tower and radix glycyrrhizae compound, are filtrated to get extract solution；
Extract solution is concentrated under reduced pressure into 0.7 times of raw material total amount, and uses bag filter mistake of the filter bag for 300 mesh filter bags
Filter, stand the concentrate after filtering and obtain filtrate；
Gained filtrate is extracted 3 times with 1-3 times of the filtrate water-saturated n-butanol measured, extracting n-butyl alcohol layer is recovered under reduced pressure molten
Agent obtains medicinal extract, and medicinal extract obtains high bioavilability Huperzine Serrate P.E 1.38kg after being crushed at being 55 DEG C in drying temperature.
Embodiment 4：
According to 1 after 15kg serrate clubmoss herbs and 15kg licorice raw materials are crushed：1 ratio mixes, and is extracted 2 times with 100 DEG C of water at atmospheric pressure,
Addition water is 9 times, extraction time 1.6h of serrate clubmoss herb and radix glycyrrhizae compound weight for the first time, and it is thousand that second, which is added water,
10 times, extraction time 1.5h of layer tower and radix glycyrrhizae compound, are filtrated to get extract solution；
Extract solution is concentrated under reduced pressure into 0.6 times of raw material total amount, and uses bag filter mistake of the filter bag for 300 mesh filter bags
Filter, stand the concentrate after filtering and obtain filtrate；
Gained filtrate is extracted 4 times with the water-saturated n-butanol of 2 times of amounts of filtrate, solvent is recovered under reduced pressure in extracting n-butyl alcohol layer
Medicinal extract is obtained, medicinal extract obtains high bioavilability Huperzine Serrate P.E 1.5kg after being crushed at being 60 DEG C in drying temperature.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. the preparation method of high bioavilability Huperzine Serrate P.E, comprises the following steps：
(1) mixed according to a certain percentage after serrate clubmoss herb is crushed with licorice raw material, extracted with water at atmospheric pressure, be filtrated to get extract solution；
(2) extract solution is concentrated under reduced pressure into a certain amount of, and the concentrate stood after filtering obtains filtrate；
(3) gained filtrate being extracted with a certain amount of water-saturated n-butanol, extracting n-butyl alcohol layer is recovered under reduced pressure solvent and obtains medicinal extract,
Extract dry obtains high bioavilability Huperzine Serrate P.E after crushing.
2. the preparation method of high bioavilability Huperzine Serrate P.E according to claim 1, it is characterised in that：The step
Suddenly the mixed proportion of serrate clubmoss herb and radix glycyrrhizae coarse powder is in (1):1：(1—3).
3. the preparation method of high bioavilability Huperzine Serrate P.E according to claim 1, it is characterised in that：The step
Suddenly extracting method is in (1)：Extracted 2 times with 85-100 DEG C of water, it is serrate clubmoss herb and radix glycyrrhizae compound weight to add water for the first time
6-10 times, extraction time be 1-2h, second when to add water be serrate clubmoss herb with 8-12 times of radix glycyrrhizae compound, extraction
Between be 0.5-1.5h.
4. the preparation method of high bioavilability Huperzine Serrate P.E according to claim 1, it is characterised in that：The step
Suddenly in (2), extract solution concentration amount is 0.5-1 times of raw material total amount in step (1) and measured that filter method is using bag filter mistake
Filter, the filter bag of bag filter filtering is 200-400 mesh filter bags.
5. the preparation method of high bioavilability Huperzine Serrate P.E according to claim 1, it is characterised in that：The step
Suddenly in (3), water-saturated n-butanol dosage used is 1-3 times of gained filtrate in step (2) and measured that extraction times are 1-5 times.
6. the preparation method of high bioavilability Huperzine Serrate P.E according to claim 1, it is characterised in that：The step
Suddenly in (3), between drying temperature is 40-70 DEG C.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1781907A (en) * | 2005-08-12 | 2006-06-07 | 上海同田生化技术有限公司 | Method for producing high purity huperzine A |
| CN101020660A (en) * | 2006-12-01 | 2007-08-22 | 河南太龙药业股份有限公司豫中制药厂 | Process of extracting huperzine B from plant medicine material huperzine serrate |
| CN101396380A (en) * | 2007-09-28 | 2009-04-01 | 赵守明 | Huperzia serrata extract and preparation method thereof |
| CN102702101A (en) * | 2012-05-23 | 2012-10-03 | 长沙市惠瑞生物科技有限公司 | Method for extracting huperzine A from huperzine serrate |
-
2017
- 2017-10-19 CN CN201710977045.8A patent/CN107550949A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1781907A (en) * | 2005-08-12 | 2006-06-07 | 上海同田生化技术有限公司 | Method for producing high purity huperzine A |
| CN101020660A (en) * | 2006-12-01 | 2007-08-22 | 河南太龙药业股份有限公司豫中制药厂 | Process of extracting huperzine B from plant medicine material huperzine serrate |
| CN101396380A (en) * | 2007-09-28 | 2009-04-01 | 赵守明 | Huperzia serrata extract and preparation method thereof |
| CN102702101A (en) * | 2012-05-23 | 2012-10-03 | 长沙市惠瑞生物科技有限公司 | Method for extracting huperzine A from huperzine serrate |
Non-Patent Citations (1)
| Title |
|---|
| 赵余庆等: "《食疗与保健食品原料功能因子手册》", 31 March 2013, 中国医药科技出版社 * |
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