CN107543812A - Ballast water work algae device for fast detecting and its detection method - Google Patents
Ballast water work algae device for fast detecting and its detection method Download PDFInfo
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- CN107543812A CN107543812A CN201710929502.6A CN201710929502A CN107543812A CN 107543812 A CN107543812 A CN 107543812A CN 201710929502 A CN201710929502 A CN 201710929502A CN 107543812 A CN107543812 A CN 107543812A
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Abstract
A kind of ballast water work algae device for fast detecting and its detection method, are related to detecting instrument technical field, and what is solved is the technical problem for detecting ballast water algae activity.The device includes sample room；Sample cell is provided with the sample room, an excitation source is respectively provided with left and right sides of sample room, the emergent light axis of two excitation sources intersects at sample cell, one of excitation source is pulse excitation light source, another excitation source is saturation excitation source, the photon hypothesis of the emergent ray of pulse excitation light source is every square of 0.1~1 micromole light quantum per second, the photon hypothesis of the emergent ray of saturation excitation source is every square of 2000~4000 micromole light quantum per second, a photelectric receiver for being used to receive sample room emergent ray is provided with immediately ahead of the light exit window of sample room.Device and method provided by the invention, it can quickly detect the algae activity of ballast water.
Description
Technical field
The present invention relates to detecting instrument technology, more particularly to a kind of ballast water work algae device for fast detecting and its detection side
The technology of method.
Background technology
Infringement of the marine species carried by ballast water for ship to marine environment is one of four big harm of ocean.According to estimating
Meter, 10,000,000,000 tons of ballast water is there are about every year and is shifted in the whole world, about has more than 3000 kinds of biologies daily by ballast water in the whole world
In the range of migrate, the halomereid entrained by it be mostly in the form of the young exist, be introduced in new environment
In species there are about 5%~10% can locality grow.Therefore, International Maritime Organization（IMO）Led on 2 13rd, 2004 in London
Cross《Management of Ships ' Ballast Water and deposit management and control pact》, with the discharge for preferably managing and controlling ballast water.Should
The supplemental provisions D-2 standards of pact define the performance requirement of ballast water, 10um~50um in the ballast water of clear stipulaties ship discharge
Living body biological quantity can not be more than 10/mL.
10um~50um living body biological is generally unicellular alga（That is microalgae）, because of its feature, it is difficult to in general mistake
Filter, physics and chemical method remove completely.Detection to 10um~50um live bodies microalgae quantity in ballast water for ship, it is recognized that
Standard method is fluorescent staining microscope inspection counting method.This method makes different activities by carrying out fluorescent staining to microalgae sample
Microalgae different fluorescence intensity is presented, intuitively observed by fluorescence microscope and count the quantity of live body microalgae.This method
Fluorescent staining need to be passed through, to be observed and counted by technical professional, and can only carried out in the lab, it is impossible to reach live fast
The requirement of speed detection.Further, guide is tried out according to ballast water sampling analysis（BWM.2/Circ.42）Regulation, its sampling mode
The undue delay to ship should not be caused with method, therefore its sampling and analysis must complete , And in a short time and obtain really
Cut, reliable result.
For quick, the easy detection of 10um~50um live bodies microalgae quantity in ballast water, existing a variety of devices, mostly also
It is the life or death that microalgae is distinguished with fluorescent staining, the methods of being counted with the conductance or LIF of fluidic cell is realized automatic
Count, eliminate artificial microscope inspection Counts amount, specific numerical value can be provided, but still need to use coloring agent, have secondary
Raw pollution produces, and automatic counting is more suited to the counting of comparatively high amts, to requiring that microalgae quantity is as little as less than 10/mL pressure
, it is necessary to carry out a certain proportion of concentration for load water, operating process is still numerous and diverse.
The content of the invention
For defect present in above-mentioned prior art, it is quick that the technical problems to be solved by the invention are to provide a kind of energy
Ballast water algae activity is detected, and without ballast water work algae device for fast detecting and its detection method caused by secondary pollution.
In order to solve the above-mentioned technical problem, a kind of ballast water work algae device for fast detecting provided by the present invention, its feature
It is：Including sample room；
The sample room offers each light entrance window around, and a light exit window, sample room are offered on front side of sample room
It is interior to be provided with the sample cell for being used for loading water body, the water body being used for into a sample cell transmitting is respectively provided with left and right sides of sample room
The excitation source of excitation line, the emergent light axis of two excitation sources is each passed through two light incidence rooms of sample room, and intersects
In sample cell, one of excitation source is pulse excitation light source, and another excitation source is saturation excitation source, and pulse swashs
The photon hypothesis of the emergent ray of light emitting source is every square of 0.1~1 micromole light quantum per second, saturation excitation source
The photon hypothesis of emergent ray is every square of 2000~4000 micromole light quantum per second, and the light exit window of sample room is just
Front is provided with a photelectric receiver for being used to receive sample room emergent ray.
Further, in addition to master controller, rectification filtering module, signal amplification module, analog-digital converter, and for will
Current signal is converted to the signal conversion module of voltage signal；
The electric signal output port of the photelectric receiver amplifies through signal conversion module, rectification filtering module, signal successively
Module, analog-digital converter are connected to the data collection terminal mouth of master controller；
Each excitation source is designed with a constant-current driven module for being used to drive the excitation source luminous, two of master controller
Driving signal output end mouth is coupled with the control port of the constant-current driven module of two excitation sources.
Further, the emergent light axis of described two excitation sources overlaps, and the emergent light axis of two excitation sources hangs down
Directly in the optical axis of sample room emitting light path.
Further, exciter filter, the light exit window of the sample room are provided between the excitation source and sample room
It is upper that light bar is housed, outgoing optical filter is housed in light bar.
The detection method of ballast water work algae device for fast detecting provided by the invention, it is characterised in that：Comprise the following steps that：
S1：Using pulse excitation light source to ballast water electromagnetic radiation pulse excitation light, ballast is excited using the pulse excitation light
Algae in water sample；The photon hypothesis of pulse excitation light is every square of 0.1~1 micromole light quantum per second；
S2：Fluorescence caused by the chlorophyll a in the algae in ballast water sample is gathered using photelectric receiver, and according to being adopted
The light intensity of the fluorescence of collection, calculate the algae density in ballast water sample；
S3：Using saturation excitation source to ballast water electromagnetic radiation saturation exciting light, ballast is excited using the saturation exciting light
Algae in water sample；The photon hypothesis of saturation exciting light is every square of 2000~4000 micromole light quantum per second；
S4：Fluorescence caused by the chlorophyll a of the live body algae in ballast water sample is gathered using photelectric receiver；
S5：A light intensity delta threshold is preset, calculates the fluorescence that the fluorescence that step S4 is gathered is gathered with step S2
Between light intensity difference, if the light intensity difference is more than default light intensity delta threshold, judge there is high work in ballast water sample
The frustule of property.
Ballast water work algae device for fast detecting provided by the invention and its detection method, according to the fluorescent effect of chlorophyll a
Come measure the algae density of ballast water and algae activity, whole measurement process do not have to coloring agent, do not destroy cell, without extraction,
There is no secondary pollution generation, without loaded down with trivial details sample handling processes, there is the characteristics of detection speed is fast.
Brief description of the drawings
Fig. 1 is the structural representation of the ballast water work algae device for fast detecting of the embodiment of the present invention.
Embodiment
Embodiments of the invention are described in further detail below in conjunction with brief description of the drawings, but the present embodiment is not used to limit
The system present invention, every similar structure using the present invention and its similar change, all should be included in protection scope of the present invention, the present invention
In pause mark represent the relation of sum.
The algae device for fast detecting as shown in figure 1, a kind of ballast water that the embodiment of the present invention is provided is lived, it is characterised in that：
Including master controller 8, sample room 1, rectification filtering module 5, signal amplification module 6, analog-digital converter 7, and for electric current to be believed
Number be converted to the signal conversion module 4 of voltage signal；
The sample room 1 offers each light entrance window around, and a light exit window, sample room are offered on front side of sample room
It is interior to be provided with the sample cell for being used for loading water body, the water body being used for into a sample cell transmitting is respectively provided with left and right sides of sample room
The excitation source 21,22 of excitation line, the emergent light axis of two excitation sources are each passed through two light incidence rooms of sample room, and
Sample cell is intersected at, one of excitation source 21 is pulse excitation light source, and another excitation source 22 is saturation exciting light
Source, the photon hypothesis of the emergent ray of pulse excitation light source are every square of 0.1~1 micromole light quantum per second, saturation
The photon hypothesis of the emergent ray of excitation source is every square of 2000~4000 micromole light quantum per second, sample room
A photelectric receiver 3 for being used to receive sample room emergent ray is provided with immediately ahead of light exit window；
The electric signal output port of the photelectric receiver 3 is put through signal conversion module 4, rectification filtering module 5, signal successively
Big module 6, analog-digital converter 7 are connected to the data collection terminal mouth of master controller 8；
Each excitation source is designed with a constant-current driven module 9 for being used to drive the excitation source luminous, and the two of master controller 8
Individual driving signal output end mouth is coupled with the control port of the constant-current driven module 9 of two excitation sources.
In the embodiment of the present invention, the emergent light axis of described two excitation sources 21,22 overlaps, and two excitation sources
Optical axis of the emergent light axis perpendicular to sample room emitting light path.
In the embodiment of the present invention, exciter filter 12 is provided between the excitation source and sample room, the sample room
Light bar 14 is housed in light exit window, outgoing optical filter 13 is housed, light bar is to block from the spuious of excitation source in light bar 14
Light disturbs.
Also include memory module 10, outlet terminal 11 in the embodiment of the present invention, the memory module passes through data wire and master
Controller interconnect, the outlet terminal by data wire or order wire be connected to master controller, outlet terminal can be display,
Serial communication device, Ethernet equipment energy device.
In the embodiment of the present invention, the master controller, rectification filtering module, signal amplification module, signal conversion module, perseverance
It is prior art to flow drive module, and master controller is using single-chip microcomputer.
The operation principle of the embodiment of the present invention is as follows：
Algae in ballast water contains chlorophyll a, by the measurement to the chlorophyll a in algae it is known that the algae in ballast water
Class density, the chlorophyll a in live body algae has photosynthesis function, and the chlorophyll a in the frustule body of dead does not have
There is photosynthetic function, therefore by measuring whether the chlorophyll a in algae has photosynthetic ability, algae can be found out
Whether class is live body, with reference to both measurement results it is known that active to the algae in ballast water.
Main controller controls constant-current driven module is utilized during measurement, drives excitation source to send out in turn by constant-current driven module
Light, it is allowed to the ballast water electromagnetic radiation excitation line being loaded in sample cell so that the leaf of the algae in ballast water sample is green
Plain a produces fluorescent effect, then collects fluorescence produced by the chlorophyll a of algae using photelectric receiver, and by the fluorescence of collection
Electric signal is converted to, then is changed by signal（Current signal is converted to voltage signal）, rectifying and wave-filtering, signal amplification after be sent into mould
Number converter is converted to data signal, is then re-fed into master controller and carries out data analysis, and analysis result is stored in memory module, and
Export to outlet terminal.
The ballast water work algae quick determination method that the embodiment of the present invention is provided, is comprised the following steps that：
S1：Using pulse excitation light source to ballast water electromagnetic radiation pulse excitation light, ballast is excited using the pulse excitation light
Algae in water sample；
The photon hypothesis of pulse excitation light is every square of 0.1~1 micromole light quantum per second so that live body algae with it is dead
The chlorophyll a of body algae can produce fluorescent effect, and now fluorescence caused by the chlorophyll a of algae is relatively weak；
S2：Fluorescence caused by the chlorophyll a in the algae in ballast water sample is gathered using photelectric receiver, and according to being adopted
The light intensity of the fluorescence of collection, the algae density in ballast water sample is calculated, the method that algae density is calculated according to the light intensity of fluorescence
For prior art；
S3：Using saturation excitation source to ballast water electromagnetic radiation saturation exciting light, ballast is excited using the saturation exciting light
Algae in water sample；
The photon hypothesis of saturation exciting light is every square of 2000~4000 micromole light quantum per second so that live body algae
Chloroplaset Inner Photosystem Is I in electronic gate close, photochemical reaction is interrupted, and makes fluorescent effect caused by chlorophyll a reach rapidly
Chlorophyll a to maximum, and dead volume algae will not produce this fluorescent effect, now caused by the chlorophyll a of live body algae
Fluorescence is relatively strong；
S4：Fluorescence caused by the chlorophyll a of the live body algae in ballast water sample is gathered using photelectric receiver；
S5：A light intensity delta threshold is preset, calculates the fluorescence that the fluorescence that step S4 is gathered is gathered with step S2
Between light intensity difference, if the light intensity difference is more than default light intensity delta threshold, judge there is high work in ballast water sample
The frustule of property, 0.2 times of the fluorescence largest light intensity that the typical value of light intensity delta threshold is excited by saturation exciting light.
, should be first by this ballast water sample if the material containing more than 50um in ballast water sample in the embodiment of the present invention
Product detect again after 50um strainer filtering, to ensure the density and activity of the algae for being less than 50um detected.
Claims (5)
- The algae device for fast detecting 1. a kind of ballast water is lived, it is characterised in that：Including sample room；The sample room offers each light entrance window around, and a light exit window, sample room are offered on front side of sample room It is interior to be provided with the sample cell for being used for loading water body, the water body being used for into a sample cell transmitting is respectively provided with left and right sides of sample room The excitation source of excitation line, the emergent light axis of two excitation sources is each passed through two light incidence rooms of sample room, and intersects In sample cell, one of excitation source is pulse excitation light source, and another excitation source is saturation excitation source, and pulse swashs The photon hypothesis of the emergent ray of light emitting source is every square of 0.1~1 micromole light quantum per second, saturation excitation source The photon hypothesis of emergent ray is every square of 2000~4000 micromole light quantum per second, and the light exit window of sample room is just Front is provided with a photelectric receiver for being used to receive sample room emergent ray.
- The algae device for fast detecting 2. ballast water according to claim 1 is lived, it is characterised in that：Also include master controller, whole Filtration module, signal amplification module, analog-digital converter are flowed, and for current signal to be converted to the signal modulus of conversion of voltage signal Block；The electric signal output port of the photelectric receiver amplifies through signal conversion module, rectification filtering module, signal successively Module, analog-digital converter are connected to the data collection terminal mouth of master controller；Each excitation source is designed with a constant-current driven module for being used to drive the excitation source luminous, two of master controller Driving signal output end mouth is coupled with the control port of the constant-current driven module of two excitation sources.
- The algae device for fast detecting 3. ballast water according to claim 1 is lived, it is characterised in that：Described two excitation sources Emergent light axis overlaps, and the emergent light axis of two excitation sources is perpendicular to the optical axis of sample room emitting light path.
- The algae device for fast detecting 4. ballast water according to claim 1 is lived, it is characterised in that：The excitation source and sample Exciter filter is provided between room, light bar is housed in the light exit window of the sample room, outgoing optical filter is housed in light bar.
- The detection method of algae device for fast detecting 5. ballast water according to claim 1 is lived, it is characterised in that：Specific steps It is as follows：S1：Using pulse excitation light source to ballast water electromagnetic radiation pulse excitation light, ballast is excited using the pulse excitation light Algae in water sample；The photon hypothesis of pulse excitation light is every square of 0.1~1 micromole light quantum per second；S2：Fluorescence caused by the chlorophyll a in the algae in ballast water sample is gathered using photelectric receiver, and according to being adopted The light intensity of the fluorescence of collection, calculate the algae density in ballast water sample；S3：Using saturation excitation source to ballast water electromagnetic radiation saturation exciting light, ballast is excited using the saturation exciting light Algae in water sample；The photon hypothesis of saturation exciting light is every square of 2000~4000 micromole light quantum per second；S4：Fluorescence caused by the chlorophyll a of the live body algae in ballast water sample is gathered using photelectric receiver；S5：A light intensity delta threshold is preset, calculates the fluorescence that the fluorescence that step S4 is gathered is gathered with step S2 Between light intensity difference, if the light intensity difference is more than default light intensity delta threshold, judge there is high work in ballast water sample The frustule of property.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109470672A (en) * | 2018-12-06 | 2019-03-15 | 大连海事大学 | The active apparatus and method of more single microalgae cells of light intensity excitation-detection |
| CN112782137A (en) * | 2020-12-24 | 2021-05-11 | 自然资源部第一海洋研究所 | Detection method of living plankton living cell flow cytometer with 10-50 mu m |
| CN113866072A (en) * | 2021-09-15 | 2021-12-31 | 中国科学院合肥物质科学研究院 | Alga living body cell number analysis method based on variable fluorescence statistical distribution |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101512321A (en) * | 2006-09-01 | 2009-08-19 | Rwo有限公司 | Method and apparatus for the detection of living phytoplankton cells in water |
| CN102539394A (en) * | 2011-09-14 | 2012-07-04 | 中国科学院安徽光学精密机械研究所 | Device and method for carrying out in-situ detection on photosynthesis activity of algae in water body based on fluorescence method |
| CN103234949A (en) * | 2013-01-30 | 2013-08-07 | 大连海事大学 | Microalgae activity detection method and device in ship ballast water |
| WO2016071356A1 (en) * | 2014-11-05 | 2016-05-12 | Medico-Chemical Lab. Aps | A ballast water analysis system |
| CN106442920A (en) * | 2016-11-11 | 2017-02-22 | 大连海事大学 | Ship ballast water drainage real-time monitoring device and method |
| CN207263635U (en) * | 2017-10-09 | 2018-04-20 | 上海欧陆科仪有限公司 | Ballast water work algae device for fast detecting |
-
2017
- 2017-10-09 CN CN201710929502.6A patent/CN107543812A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101512321A (en) * | 2006-09-01 | 2009-08-19 | Rwo有限公司 | Method and apparatus for the detection of living phytoplankton cells in water |
| US20090305330A1 (en) * | 2006-09-01 | 2009-12-10 | Bernd Kroon | Method and apparatus for the detection of living phytoplankton cells in water |
| CN102539394A (en) * | 2011-09-14 | 2012-07-04 | 中国科学院安徽光学精密机械研究所 | Device and method for carrying out in-situ detection on photosynthesis activity of algae in water body based on fluorescence method |
| CN103234949A (en) * | 2013-01-30 | 2013-08-07 | 大连海事大学 | Microalgae activity detection method and device in ship ballast water |
| WO2016071356A1 (en) * | 2014-11-05 | 2016-05-12 | Medico-Chemical Lab. Aps | A ballast water analysis system |
| CN106442920A (en) * | 2016-11-11 | 2017-02-22 | 大连海事大学 | Ship ballast water drainage real-time monitoring device and method |
| CN207263635U (en) * | 2017-10-09 | 2018-04-20 | 上海欧陆科仪有限公司 | Ballast water work algae device for fast detecting |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109470672A (en) * | 2018-12-06 | 2019-03-15 | 大连海事大学 | The active apparatus and method of more single microalgae cells of light intensity excitation-detection |
| CN112782137A (en) * | 2020-12-24 | 2021-05-11 | 自然资源部第一海洋研究所 | Detection method of living plankton living cell flow cytometer with 10-50 mu m |
| CN113866072A (en) * | 2021-09-15 | 2021-12-31 | 中国科学院合肥物质科学研究院 | Alga living body cell number analysis method based on variable fluorescence statistical distribution |
| CN113866072B (en) * | 2021-09-15 | 2023-08-11 | 中国科学院合肥物质科学研究院 | Algae living cell number analysis method based on variable fluorescence statistical distribution |
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