CN107519479A - A kind of soluble powder of the peptide containing enterobacteria and preparation method thereof - Google Patents
A kind of soluble powder of the peptide containing enterobacteria and preparation method thereof Download PDFInfo
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- CN107519479A CN107519479A CN201710985369.6A CN201710985369A CN107519479A CN 107519479 A CN107519479 A CN 107519479A CN 201710985369 A CN201710985369 A CN 201710985369A CN 107519479 A CN107519479 A CN 107519479A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/146—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic macromolecular compounds
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses a kind of soluble powder of peptide containing enterobacteria, the weight composition of every 100 parts of medicines is：1 10 parts of enterobacteria peptide, 1 20 parts of beta cyclodextrin, surplus is water soluble starch；Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and depositary institution is referred to as：CGMCC, depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：ETEC.The present invention uses inclusion technique, and inclusion processing is carried out to enterobacteria peptide using beta cyclodextrin and water soluble starch, on the one hand increases its solubility, on the other hand increases the stability of polypeptide drug.
Description
Technical field
The invention belongs to biological peptide technical field, and in particular to a kind of soluble powder of peptide containing enterobacteria and its preparation side
Method.
Background technology
Recently as a large amount of uses of antibiotic, bacterial drug resistance is constantly formed, and causes existing antibacterials to bacterium
The effect for the treatment of of infection, is low or invalid, the harm getting worse of formation.How to overcome the drug resistance of bacterium is current research
Emphasis and focus, researchers are just making great efforts to seek new antibacterial strategy, and there is an urgent need to develop new antibiotic preparation for this.
Efficiently, low toxicity, be not easy to be formed the antibacterial peptide of drug resistance as being most hopeful to replace the new drug preparation of antibiotic by domestic surgery
Grind the concern of worker.
For the purpose of the safe biological peptide Co., Ltd of Linzhou City middle peasant grain husk produces the antibacterial peptide bacterial strain of anti-Gram-negative bacteria to screen, lead to
Outdoor sampling is crossed, bacterial strain primary dcreening operation, is filtered out altogether with 25 plants of antibacterial activity bacterial strain, obtaining one plant through excessive wheel secondary screening has preferably
The bacterial strain of bacteriostatic activity, it is defined as prebiotic Escherichia coli ECN by strain idenfication.Antibacterial substance caused by the bacterial strain is entered simultaneously
Some properties evaluations and induction mutation of bacterium are gone, have greatly improved the production antibacterial peptide ability of bacterial strain, industry can be met
The application demand of production.
Enterobacteria peptide scope of restraining fungi is wide, to treatment poultry, fowl intestines problem positive effect.For the application method of poultry, fowl, adopt
Take be administered orally it is convenient and easy, cost is relatively low.Due to the poorly water-soluble of enterobacteria peptide, by factors such as external condition temperature, pH value
Influence, make oral formulations and have certain limitation.
The content of the invention
The technical problem to be solved in the present invention is the poorly water-soluble of enterobacteria peptide, by factors such as external condition temperature, pH value
Influence, make oral formulations and have certain limitation, to solve the above problems, the present invention provides a kind of the solvable of peptide containing enterobacteria
Property powder and preparation method thereof.
The purpose of the present invention is realized in the following manner：
A kind of soluble powder of peptide containing enterobacteria, the weight composition of every 100 parts of medicines are：Enterobacteria peptide 1-10 parts, beta-schardinger dextrin
1-20 parts, surplus are water soluble starch.
The enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in the micro- life of China
Thing culture presevation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Beijing is exposed to the sun
The institute 3 of area North Star West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, classification life
Name：ETEC.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 10-20% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:（5-7）；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, and temperature is 40-60 DEG C, under agitation, first
Beta-schardinger dextrin is dissolved in ethanol water, then enterobacteria peptide is added in ethanol water, after stirring 10-30min, cooling
To room temperature；
（4）By step（3）Gained carries out frozen dried 8-12h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 60-80 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Relative to prior art, the present invention uses inclusion technique, using beta-schardinger dextrin and water soluble starch to enterobacteria peptide
Inclusion processing is carried out, on the one hand increases its solubility, on the other hand increases the stability of polypeptide drug.
Brief description of the drawings
Fig. 1 is solubility curve comparison diagram.
Embodiment
Embodiment 1：
A kind of soluble powder of peptide containing enterobacteria, the weight composition of every 100 parts of medicines are：Enterobacteria peptide 1-10 parts, beta-schardinger dextrin
1-20 parts, surplus are water soluble starch.
To room temperature
Thing culture presevation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Beijing is exposed to the sun
The institute 3 of area North Star West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, classification life
Name：ETEC.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 10-20% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:（5-7）；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, and temperature is 40-60 DEG C, under agitation, first
Beta-schardinger dextrin is dissolved in ethanol water, then enterobacteria peptide is added in ethanol water, after stirring 10-30min, cooling
To room temperature；
（4）By step（3）Gained carries out frozen dried 8-12h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 60-80 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Embodiment 2：
A kind of soluble powder of peptide containing enterobacteria, the weight composition per 100g medicines are：Enterobacteria peptide 1g, beta-schardinger dextrin 1g, it is remaining
Measure as water soluble starch.
Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：Greatly
Intestines Escherichia.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 10% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:5；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, temperature be 40 DEG C, under agitation, first by β-
Cyclodextrin is added in ethanol water in ethanol water, then by enterobacteria peptide, after stirring 10min, is cooled to room temperature；
（4）By step（3）Gained carries out frozen dried 8h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 60 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Embodiment 3：
A kind of soluble powder of peptide containing enterobacteria, the weight composition per 100g medicines are：Enterobacteria peptide 2g, beta-schardinger dextrin 5g, it is remaining
Measure as water soluble starch.
Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：Greatly
Intestines Escherichia.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 12% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:5.5；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, temperature be 44 DEG C, under agitation, first by β-
Cyclodextrin is added in ethanol water in ethanol water, then by enterobacteria peptide, after stirring 14min, is cooled to room temperature；
（4）By step（3）Gained carries out frozen dried 9h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 64 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Embodiment 4：
A kind of soluble powder of peptide containing enterobacteria, the weight composition per 100g medicines are：Enterobacteria peptide 4g, beta-schardinger dextrin 10g, it is remaining
Measure as water soluble starch.
Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：Greatly
Intestines Escherichia.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 14% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:6；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, temperature be 48 DEG C, under agitation, first by β-
Cyclodextrin is added in ethanol water in ethanol water, then by enterobacteria peptide, after stirring 18min, is cooled to room temperature；
（4）By step（3）Gained carries out frozen dried 10h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 68 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Embodiment 5：
A kind of soluble powder of peptide containing enterobacteria, the weight composition per 100g medicines are：Enterobacteria peptide 6g, beta-schardinger dextrin 12g, it is remaining
Measure as water soluble starch.
Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：Greatly
Intestines Escherichia.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 16% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:6.5；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, temperature be 52 DEG C, under agitation, first by β-
Cyclodextrin is added in ethanol water in ethanol water, then by enterobacteria peptide, after stirring 22min, is cooled to room temperature；
（4）By step（3）Gained carries out frozen dried 11h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 72 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Embodiment 6：
A kind of soluble powder of peptide containing enterobacteria, the weight composition per 100g medicines are：Enterobacteria peptide 8g, beta-schardinger dextrin 15g, it is remaining
Measure as water soluble starch.
Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：Greatly
Intestines Escherichia.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 18% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:7；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, temperature be 56 DEG C, under agitation, first by β-
Cyclodextrin is added in ethanol water in ethanol water, then by enterobacteria peptide, after stirring 26min, is cooled to room temperature；
（4）By step（3）Gained carries out frozen dried 12h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 76 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Embodiment 7：
A kind of soluble powder of peptide containing enterobacteria, the weight composition per 100g medicines are：Enterobacteria peptide 10g, beta-schardinger dextrin 20g,
Surplus is water soluble starch.
Enterobacteria peptide is Escherichia coli ZNYT2 metabolite, and the Escherichia coli ZNYT2 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, depositary institution is referred to as：CGMCC, depositary institution address：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, preservation date：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：Greatly
Intestines Escherichia.
The preparation method of the soluble powder of the peptide containing enterobacteria described above, is comprised the following steps that：
（1）Dose volume fraction is 20% ethanol water；
（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation
The mass ratio of water is 1:7；
（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, temperature be 60 DEG C, under agitation, first by β-
Cyclodextrin is added in ethanol water in ethanol water, then by enterobacteria peptide, after stirring 30min, is cooled to room temperature；
（4）By step（3）Gained carries out frozen dried 12h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；
（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 80 mesh；
（6）Mixing, packing, produces enterobacteria peptide soluble powder.
Escherichia coli ZNYT2 can be utilized to produce enterobacteria peptide by conventional method, can also be utilized by following steps
Escherichia coli ZNYT2 production enterobacteria peptides described above：
（1）The separation of strain：By the enrichment to outdoor collecting sample and primary dcreening operation, secondary screening, screen and produce from primary dcreening operation bacterial strain
Raw antibacterial substance can be good at being resistant to high temperature and Protease Treatment, and strain growth is rapid, and inhibition zone is big and obvious
Bacterial strain S49-2, it is named as prebiotic Escherichia coli ZNYT2；
（2）The mutagenesis of strain：After isolated prebiotic Escherichia coli ZNYT2 original strains are expanded into culture, it is configured to bacterium and hangs
After liquid, mutagenesis is carried out to the bacterial strain in bacteria suspension first with He-Ne Lasers, by cultivating and screening, chooses fast growing, transparent
The big bacterial strain of loop diameter, recycle ultraviolet nitrosoguanidine to carry out complex mutation, by culture and primary dcreening operation, choose fast growing, thoroughly
The big bacterial strain of bright loop diameter, secondary screening is carried out, obtains stable, high-yielding bacterial strain；
（3）The expansion culture of strain
By step（2）In obtained stable, high-yielding Escherichia coli ZNYT2 carry out actication of culture, Shaking culture and seed tank culture,
Obtain seed fermentation liquid；
（4）Fermentation cylinder for fermentation culture
By step（3）In obtained seed fermentation liquid be inoculated in the fermentation tank culture medium in fermentation tank, carry out fermented and cultured and obtain
Obtain zymotic fluid；
（5）To step（4）In obtained zymotic fluid post-processed, obtain enterobacteria peptide
A. economic benefits and social benefits concentrate；Concentrated under vacuum using Double-effect separator；
B. alcohol precipitation：It is 65%-85% that the alcohol content of zymotic fluid after economic benefits and social benefits are concentrated, which is adjusted to volume fraction, and alcohol is carried out at 4 DEG C
It is heavy；
C. centrifuge：Zymotic fluid after alcohol precipitation is centrifuged, collects supernatant；
D. decolourize：Supernatant after centrifugation is decolourized by aluminum oxide diacolation post；
E. alcohol reclaims：The ethanol in the zymotic fluid after decolouring is reclaimed to without alcohol taste；
F. saltout：Ammonium sulfate is added into the zymotic fluid after alcohol reclaims, becomes edged stirring, continues to stir after adding ammonium sulfate
1-2h is mixed, stands 12-15h, precipitation is collected in centrifugation, and the salting-out separation of polypeptide uses saturated ammonium sulfate solwution method；According to ammonium sulfate
Saturation degree solution table understands that 1L zymotic fluids add 767g ammonium sulfate solids；
G. micro-filtration：Salt precipitation will be collected into be redissolved, solvent is the ethanol solution that volume fraction is 20%, then by after redissolution
Solution pass through 0.45 μm of micro-filtration filter membrane and carry out micro-filtration, collect micro-filtrate；
H. column chromatography for separation：Column chromatography for separation is carried out to micro-filtrate using chromatographic column, using linear gradient elution method, collects eluent；
I. ultrafiltration：The eluent of column chromatography is subjected to ultrafiltration by 1000Da milipore filters, collects trapped fluid；
J. freeze：Cryogenic vacuum freeze-drying is carried out to the trapped fluid after ultrafiltration, produces enterobacteria peptide sterling.
The step（5）In h steps in chromatographic column used be BPG200/500 chromatographic columns.
The step（5）In h steps in column chromatography for separation concretely comprise the following steps：
（1）First balanced with pure water rinsing pillar to baseline；Buffer A rinse pillar 2CV；
（2）Load sample, applied sample amount are one times of column volume；
（3）Buffer A balance pillars 3CV；
（4）Buffer B 0-100% linear gradient elutions, the change of observation conductance and spectrogram, collect object mass peak；
（5）Buffer B 3CV rinse pillar untill baseline and conductance drop to pure water conductance, prepare loading next time；
Buffer solution： BufferA：1M/L sodium chloride；BufferB：Pure water；
The preparation of sample solution：Enterobacteria peptide is that 20% ethanol solution is dissolved with volume fraction, and electricity is adjusted with medicinal grade sodium chloride
It is directed at 55ms/cm.
The solubility comparing result of enterobacteria peptide soluble powder prepared by enterobacteria peptide and the embodiment of the present invention 5 in water is such as
Shown in table 1 and Fig. 1.
From the point of view of dissolubility contrast test：Enterobacteria peptide solubility in water is low, and liter is had with the pH of aqueous solution increase
Height, and the stability of enterobacteria peptide soluble powder is high, solubility significantly increases, and the pH of the aqueous solution influences less on its solubility.
Above-described is only the preferred embodiment of the present invention, it is noted that for those skilled in the art,
Under the premise of general idea of the present invention is not departed from, some changes and improvements can also be made, these should also be considered as the present invention's
Protection domain.
Claims (3)
- A kind of 1. soluble powder of peptide containing enterobacteria, it is characterised in that：The weight of every 100 parts of medicines forms：Enterobacteria peptide 1- 10 parts, beta-schardinger dextrin 1-20 parts, surplus is water soluble starch.
- 2. the soluble powder of the peptide according to claim 1 containing enterobacteria, it is characterised in that：The enterobacteria peptide is large intestine Bacillus ZNYT2 metabolite, it is commonly micro- that the Escherichia coli ZNYT2 is preserved in China Committee for Culture Collection of Microorganisms Bio-Centers, depositary institution is referred to as：CGMCC, depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation day Phase：On August 23rd, 2016, deposit number are：CGMCC NO. 12902, Classification And Nomenclature：ETEC.
- 3. the preparation method of the soluble powder of the peptide containing enterobacteria as claimed in claim 1, it is characterised in that：Specific steps are such as Under：（1）Dose volume fraction is 10-20% ethanol water；（2）Weigh the enterobacteria peptide and beta-schardinger dextrin, beta-schardinger dextrin and step of formula ratio（1）In the ethanol water of middle preparation The mass ratio of water is 1:（5-7）；（3）By step（1）The ethanol water of middle preparation is placed in agitator tank, and temperature is 40-60 DEG C, under agitation, first Beta-schardinger dextrin is dissolved in ethanol water, then enterobacteria peptide is added in ethanol water, after stirring 10-30min, cooling To room temperature；（4）By step（3）Gained carries out frozen dried 8-12h containing the ethanol water of enterobacteria peptide and beta-schardinger dextrin；（5）By step（4）Obtained freeze-drying inclusion compound crushes after being mixed with water soluble starch, and granularity is 60-80 mesh；（6）Mixing, packing, produces enterobacteria peptide soluble powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110575532A (en) * | 2018-06-08 | 2019-12-17 | 上海莫息生物科技有限公司 | Nosiheptide soluble powder and preparation method thereof |
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| CN106497859A (en) * | 2016-12-09 | 2017-03-15 | 中国科学院广州能源研究所 | A kind of method that utilization conductive carrier tames propanoic acid methanogen film |
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| US20080200374A1 (en) * | 2005-03-09 | 2008-08-21 | Rutgers, The State University Of New Jersey | Mutational derivatives of microcin j25 |
| CN101584854A (en) * | 2008-05-22 | 2009-11-25 | 北京嘉事联博医药科技有限公司 | Romurtide cyclodextrin inclusion compound, preparation thereof and method for preparing same |
| CN106497859A (en) * | 2016-12-09 | 2017-03-15 | 中国科学院广州能源研究所 | A kind of method that utilization conductive carrier tames propanoic acid methanogen film |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110575532A (en) * | 2018-06-08 | 2019-12-17 | 上海莫息生物科技有限公司 | Nosiheptide soluble powder and preparation method thereof |
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