CN106497859A - A kind of method that utilization conductive carrier tames propanoic acid methanogen film - Google Patents
A kind of method that utilization conductive carrier tames propanoic acid methanogen film Download PDFInfo
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- CN106497859A CN106497859A CN201611127388.7A CN201611127388A CN106497859A CN 106497859 A CN106497859 A CN 106497859A CN 201611127388 A CN201611127388 A CN 201611127388A CN 106497859 A CN106497859 A CN 106497859A
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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Abstract
The invention discloses a kind of method that utilization conductive carrier tames propanoic acid methanogen film，The method arranges conductive carrier in anaerobic fermentation system，With propanoic acid and sodium propionate as carbon source，With biogas fermentative liquid as inoculum，By steps raising organic loading to 3.0g/L，The simultaneously steps propanoic acid that improves obtains organic loading more than 3.0g/L with the mol ratio of sodium propionate to 4、Methanogen film of the theoretical methane production rate more than 70%，It is applied in anaerobic fermentation system to release the thermodynamics restriction that the factors such as hydrogen dividing potential drop are brought，Effectively improve propionic acid degradation efficiency，Effectively alleviate the fermentation inhibitory that propionic acid accumulation brings，Improve the stability of anaerobic fermentation system，Improve anaerobic fermentation efficiency，Reduce anaerobic fermentation to become sour probability，Its factor of created gase for theoretical yield 75%，The utilization rate of propanoic acid and acetic acid is more than 90%.
Description
Technical field：
The present invention relates to fermentable production clean energy technology field, and in particular to a kind of using conductive carrier domestication
The method of propanoic acid methanogen film.
Background technology：
Anaerobic fermentation prepares biological flue gas technology and is known as maximally effective organic waste treatment skill by countries in the world
Art, is especially suitable for processing that moisture content is higher and readily biodegradable Organic substance.Biomass are prepared as raw material with organic waste
Combustion gas, can reduce the discharge of pollutant, and its clean gas for producing substitutes the consumption of fossil energy and forest fuel wood again, realize
Further reduce discharging, with two-way cleaning function, be the major action for tackling climate change.Easily occur in anaerobic fermentation process
Sour suppression situation, causes system unstability, and factor of created gase reduces, fermentation period is elongated, or even fermentation failure.Acid suppresses to be usually associated with
The accumulation of propanoic acid, according to thermodynamic analysis, the degraded of propanoic acid because by the factors such as hydrogen dividing potential drop affected to be difficult to spontaneous carry out, only big
Can realize degrading in the presence of amount symbiosis strain, therefore propionic acid degradation is the rate-limiting step of anaerobic fermentation.The accumulation of propanoic acid
Cause fermentation system pH to reduce, it is suppressed that the activity of methanogen, ultimately result in fermentation system gas deliverability and decline or occur
Acidifying.Common solution is macroscopically to optimize operational factor, such as adds alkali and adjusts pH, or reduces organic loading and fermentation
Concentration, makes fermentation run under relatively low fermentation concentration, limits fermentation efficiency.As biological reinforcing technology is at waste water
The application in reason field, research worker begin attempt to apply it in marsh gas fermenting system, i.e., artificial interpolation specific function
Microorganism improves the stability of fermentation system tackling sour suppression.But feeding function flora mostly is purchase pure culture and is combined at present
Microbial inoculum, environmental condition require harsh, it is difficult to adapt to complex environment and play invigoration effect.
Therefore, it is to obtain biological activity stably and the biological reinforced microbial inoculum of efficient degradation propanoic acid, it is necessary to which proposing one kind has
The domestication of effect ground and bacterial preparation process.
Content of the invention：
It is an object of the invention to provide a kind of method that utilization conductive carrier tames propanoic acid methanogen film, by detesting
Conductive carrier is set in aerobe fermentation system, with propanoic acid and sodium propionate as carbon source, domestication propanoic acid methane phase fungus strain simultaneously forms biomembrane,
Propionic acid degradation efficiency is effectively improved, deactivation system acid suppresses, and lifts the stability of anaerobic system.
The present invention is achieved by the following technical programs：
A kind of method that utilization conductive carrier tames propanoic acid methanogen film, the method are arranged in anaerobic fermentation system
Conductive carrier, with propanoic acid and sodium propionate as carbon source, with biogas fermentative liquid as inoculum, is carried with conductive carrier as biomembrane attachment
Body, by steps raising organic loading to 3.0g/L, while the steps mol ratio for improving propanoic acid and sodium propionate is to 4：1, obtain
Obtain organic loading and be more than 3.0g/L, methanogen film of the theoretical methane production rate more than 70% specifically includes following steps：In nitrogen
Gas and carbon dioxide volume ratio are 4:In 1 mixed atmosphere, it is 14 by volume:1 is inoculated into inoculum in culture medium and carries out
Culture, cultivation temperature are 37 DEG C, per 24h replacement mediums, are within 15 days a cultivation cycle, and culture adds conductive after 10-15 days
Carrier so as to be all immersed in fermentation liquid, hereafter each cultivation cycle is steps improves propanoic acid in organic loading and culture medium
With the mol ratio of sodium propionate, the mol ratio for being more than 3.0g/L, propanoic acid and sodium propionate up to organic loading is 4：1, obtain target
Product；The culture medium is formulated as follows：400mg NH are added in 1L water4Cl、250mg MgSO4·6H2O、400mg KCl、
120mgCaCl2·2H2O、80mg(NH4)2HPO4、55mg FeCl3·6H2O、5000mg NaHCO3, different mol ratio propanoic acid
With sodium propionate, CoCl is additionally added2·6H2O、NiCl2·6H2O、MnCl2·4H2O、CuCl2·2H2O、AlCl3·6H2O、ZnCl2、
Na2WO4·2H2O、H3BO3、Na2SeO3、Ma2MoO4·2H2The each 0.0005mg of O.
The conductive carrier is selected from conductive carbon felt, carbon fiber brush etc..
In anaerobic digestion process in addition to inter-species hydrogen pass through mechanism, direct inter-species electron transmission (direct interspecies
Electron transfer, DIET) mechanism is equally proved exist, and can be strengthened by adding conductive materials.Directly
Between inoculation, the acidizing product such as electron transmission (abbreviation DIET) approach degraded propanoic acid, butanoic acid can effectively evade the heating power that hydrogen dividing potential drop is brought
Learn and limit.The present invention carries out the domestication of propanoic acid methanogen in the container for be provided with conductive carrier, can strengthen methanogen
The ability of degraded propanoic acid, and biomembrane is formed on conductive carrier, the microorganism on biomembrane is by direct inter-species electron transmission
(abbreviation DIET) approach realizes the efficient degradation of propanoic acid.
The present invention also protects the application of the propanoic acid methanogen film, is applied in anaerobic fermentation system.
Beneficial effects of the present invention are as follows：
1) present invention passes through to arrange conductive carrier in anaerobic fermentation system, tames efficient propanoic acid using conductive carrier and produces first
Alkane bacterium, and biomembrane enrichment is formed it into, the microorganism on biomembrane is by direct inter-species electron transmission (abbreviation DIET) approach
The efficient degradation of propanoic acid is realized, is applied in anaerobic fermentation system to release the thermodynamics restriction that the factors such as hydrogen dividing potential drop are brought,
Propionic acid degradation efficiency is effectively improved, is effectively alleviated the fermentation inhibitory that propionic acid accumulation brings, is improved stablizing for anaerobic fermentation system
Property, anaerobic fermentation efficiency is improved, reducing anaerobic fermentation becomes sour probability, its factor of created gase for theoretical yield 75%, propanoic acid and acetic acid
Utilization rate be more than 90%.
2) the propanoic acid methanogen film that present invention domestication is obtained, can be added directly into acid and suppress to be particularly propanoic acid and acetic acid
In the fermentation system of suppression, the sour suppression phase can be shortened, improve system effectiveness and stability.
Description of the drawings：
Fig. 1 is the on-line automatic gas production culture apparatuses structural representation of the embodiment of the present invention；
Wherein, 1, computer, 2, effusion meter, the 3, first fermentation tank, the 4, second fermentation tank, 5, carbon fiber brush.
Fig. 2 is the carbon fiber brush before fermentation；
Fig. 3 is the carbon fiber brush after fermentation；
The propanoic acid methanogen film that Fig. 4 is embodiment 2 to be obtained using present invention domestication acts on changing food waste anaerobism and disappears
The design sketch of change system.
Specific embodiment：
The following is and the present invention is further illustrated, rather than limitation of the present invention.
Embodiment 1：
The culture medium is formulated as follows：400mg NH are added in 1L water4Cl、250mg MgSO4·6H2O、400mg
KCl、120mg CaCl2·2H2O、80mg(NH4)2HPO4、55mg FeCl3·6H2O、5000mg NaHCO3, different mol ratio
Propanoic acid and sodium propionate, are additionally added CoCl2·6H2O、NiCl2·6H2O、MnCl2·4H2O、CuCl2·2H2O、AlCl3·6H2O、
ZnCl2、Na2WO4·2H2O、H3BO3、Na2SeO3、Ma2MoO4·2H2The each 0.0005mg of O.
Inoculum takes from vaccary cattle manure methane-generating pit fermentation liquid, organic loading 2.0g/L, day volume yield rate be 1.1L/L
d.
Culture apparatuses adopt the on-line automatic gas production culture apparatuses of 2L CSTR as shown in Figure 1.3 He of wherein the first fermentation tank
For being all 2L anaerobic fermentation bottles, difference is to be not provided with carbon fiber brush in the first fermentation tank 3 second fermentation tank 4, the second fermentation
Tank 4 is provided with the carbon fiber brush that buy on market, and other condition of culture are identical, the daily output of the first fermentation tank 3 and the second fermentation tank 4
Tolerance is recorded and transmitted to computer 1 by effusion meter 2 and is recorded.
The method that propanoic acid methanogen film is tamed using conductive carrier is comprised the following steps：In nitrogen and carbon dioxide body
Product is than being 4:In 1 mixed atmosphere, the first fermentation tank 3 and the second fermentation tank 4 respectively take 2100ml inoculums and are inoculated into 150ml cultures
Cultivated in base, cultivation temperature is 37 DEG C, suctions out the bacterium solution of 150ml per 24h with syringe, and injects the fresh trainings of 150ml
Foster base, 15 days is a cultivation cycle, adds carbon fiber brush so as to be all immersed in fermentation liquid after cultivating 10-15 days, this
The steps mol ratio (referring to table 1) for improving propanoic acid and sodium propionate in organic loading and culture medium of each cultivation cycle afterwards, until
Organic loading is 4 more than the mol ratio of 3.0g/L, propanoic acid and sodium propionate：1, methane production rate is obtained more than the 70% of theoretical yield
Efficient propanoic acid methanogen film.
Table 1
By the domestication in 10 cycles, the raising of increase and organic loading of first fermentation tank 3 with propanoic acid ratio,
125 days (propanoic acid：The mol ratio of sodium propionate is equal to 4：1, organic loading is 2.5g/L) become sour and stop aerogenesis, and the second fermentation tank
4 obtain can steadily in the long term organic loading 3.0g/L, propanoic acid and sodium propionate mol ratio 4：Efficient utilization third under the conditions of 1
The utilization rate of sour methanogen film, its factor of created gase for theoretical yield 75%, propanoic acid and acetic acid is more than 90%.Second is fermented
Carbon fiber brush 5 in tank 4 is taken out and is observed, and as shown in Figure 3, is attached to the material of sticky shape in a large number in carbon fiber brush 5,
Through microbiological analysiss, dominant microflora is mainly Methanospirillum and two kinds of Methanosaeta thereon, and which passes through inter-species
The direct pipeline of electronics (DIET) degraded propanoic acid.
Embodiment 2：The propanoic acid methanogen film that domestication is obtained is applied to the changing food waste fermentation system of laboratory.
Changing food waste is commonly called as water from washing rice, rich in organic matter, easily rots, and forms healthy hidden danger and environmental pollution etc. and asks
Topic.With changing food waste as raw material carry out anaerobism prepare biogas can realize changing food waste into resources utilize, but in anaerobic processes,
Changing food waste is easily acidified, particularly the accumulation of propanoic acid and acetic acid, suppresses methanogenic being smoothed out.This laboratory is in TS concentration
For 12%, at 37 DEG C of middle temperature, anaerobic fermentation batch test being carried out, aerogenesis peak being reached within the 10th day in fermentation, the 15th to 25 day always
In sour inhibitory state, until the 30th day or so, stop aerogenesis.Under the same conditions, it is directly added within the 15th day the present embodiment 1 to tame and docile
Change the propanoic acid methanogen film for obtaining, system recovery aerogenesis, and aerogenesis peak, fermentation time reduction to 25 was reached at the 20th day
My god (referring to Fig. 4).
Claims (3)
1. a kind of method that utilization conductive carrier tames propanoic acid methanogen film, it is characterised in that the method is in anaerobic fermentation
Conductive carrier is set in system, with propanoic acid and sodium propionate as carbon source, with biogas fermentative liquid as inoculum, with conductive carrier as biology
Film adheres to carrier, by steps raising organic loading to 3.0g/L, while the steps mol ratio for improving propanoic acid and sodium propionate
To 4：1, obtain organic loading and be more than 3.0g/L, methanogen film of the theoretical methane production rate more than 70% is specifically included following
Step：It is 4 in nitrogen and carbon dioxide volume ratio:In 1 mixed atmosphere, it is 14 by volume:Inoculum is inoculated into culture by 1
Cultivated in base, cultivation temperature is 37 DEG C, per 24h replacement mediums, be within 15 days a cultivation cycle, after cultivating 10-15 days
Add conductive carrier so as to be all immersed in fermentation liquid, hereafter each cultivation cycle is steps improves organic loading and culture
The mol ratio of propanoic acid and sodium propionate in base, until organic loading is 4 more than the mol ratio of 3.0g/L, propanoic acid and sodium propionate：1, obtain
Obtained target product；The culture medium is formulated as follows：400mg NH are added in 1L water4Cl、250mg MgSO4·6H2O、
400mg KCl、120mg CaCl2·2H2O、80mg(NH4)2HPO4、55mg FeCl3·6H2O、5000mg NaHCO3, difference rubs
The propanoic acid and sodium propionate of your ratio, is additionally added CoCl2·6H2O、NiCl2·6H2O、MnCl2·4H2O、CuCl2·2H2O、AlCl3·
6H2O、ZnCl2、Na2WO4·2H2O、H3BO3、Na2SeO3、Ma2MoO4·2H2The each 0.0005mg of O.
2. the method that utilization conductive carrier according to claim 1 tames propanoic acid methanogen film, it is characterised in that institute
State the one kind of conductive carrier in conductive carbon felt, carbon fiber brush.
3. a kind of method of the utilization conductive carrier domestication propanoic acid methanogen film described in utilization claim 1 or 2 is obtained
The application of propanoic acid methanogen film.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107519479A (en) * | 2017-10-20 | 2017-12-29 | 林州中农颖泰生物肽有限公司 | A kind of soluble powder of the peptide containing enterobacteria and preparation method thereof |
| CN108529748A (en) * | 2018-04-25 | 2018-09-14 | 浙江工业大学膜分离与水处理协同创新中心湖州研究院 | Synchronous nitration and denitrification bioreactor |
| CN109321444A (en) * | 2018-11-01 | 2019-02-12 | 重庆大学 | The method and biofilm reactor of two step anaerobic fermentation preparing hydrogen alkane gas of streaming drop |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101768562A (en) * | 2009-12-31 | 2010-07-07 | 哈尔滨工业大学 | Separation method of monoprop-oxidating hydrogen-producing acetogenic dominant bacterial flora |
| CN106085926A (en) * | 2016-08-25 | 2016-11-09 | 农业部沼气科学研究所 | Compound bacteria of Rapid reversal propionic acid accumulation and its preparation method and application |
-
2016
- 2016-12-09 CN CN201611127388.7A patent/CN106497859A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768562A (en) * | 2009-12-31 | 2010-07-07 | 哈尔滨工业大学 | Separation method of monoprop-oxidating hydrogen-producing acetogenic dominant bacterial flora |
| CN106085926A (en) * | 2016-08-25 | 2016-11-09 | 农业部沼气科学研究所 | Compound bacteria of Rapid reversal propionic acid accumulation and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 徐恒等: "颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究", 《中国环境科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107519479A (en) * | 2017-10-20 | 2017-12-29 | 林州中农颖泰生物肽有限公司 | A kind of soluble powder of the peptide containing enterobacteria and preparation method thereof |
| CN107519479B (en) * | 2017-10-20 | 2020-12-08 | 林州中农颖泰生物肽有限公司 | Soluble powder containing enterobacter peptide and preparation method thereof |
| CN108529748A (en) * | 2018-04-25 | 2018-09-14 | 浙江工业大学膜分离与水处理协同创新中心湖州研究院 | Synchronous nitration and denitrification bioreactor |
| CN109321444A (en) * | 2018-11-01 | 2019-02-12 | 重庆大学 | The method and biofilm reactor of two step anaerobic fermentation preparing hydrogen alkane gas of streaming drop |
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Application publication date: 20170315 |