CN106867926A - A kind of culture medium for streptococcus pneumonia - Google Patents
A kind of culture medium for streptococcus pneumonia Download PDFInfo
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- CN106867926A CN106867926A CN201510929439.7A CN201510929439A CN106867926A CN 106867926 A CN106867926 A CN 106867926A CN 201510929439 A CN201510929439 A CN 201510929439A CN 106867926 A CN106867926 A CN 106867926A
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- culture medium
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- streptococcus pneumonia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Genetics & Genomics (AREA)
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- Organic Chemistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a kind of culture medium for streptococcus pneumonia, the culture medium includes the composition of following weight portion：8 parts of starch, 6 parts of sucrose, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, 1 part of the tuber of dwarf lilyturf；Cysteine, casein or the glucose of specified quantitative can also be further added on this basis.The formula with the addition of certain Chinese medicine composition on the basis of culture medium is normal with material, surprisingly obtain prominent culture effect, be particularly suited for the culture of streptococcus pneumonia, and application prospect is good.
Description
Technical field
The present invention relates to microbial technology field, and in particular to a kind of culture medium for streptococcus pneumonia.
Background technology
Streptococcus pneumonia was distinguished by Pasteur (Louis Pasteur) and G.M.Sternberg first in 1881
Isolated from patient's sputum in France and the U.S..Be that Grain stain is positive, thalline like spearhead shape, it is in pairs or into
The diplococcus of short catenation, there is the pod membrane that chemical composition is polysaccharide outside toxic strain thalline.5%~10% is normal
This bacterium is carried in people's upper respiratory tract, toxic strain is the important pathogen for causing human diseases.The bacterial strain is in biological skill
Art field has certain application, but streptococcus pneumonia growth rate is slower in conventional culture medium, while
Easily there is microbiological contamination phenomenon.
The content of the invention
It is contemplated that for the technological deficiency of prior art, there is provided a kind of culture medium for streptococcus pneumonia,
To solve the slower technical problem of streptococcus pneumonia growth rate in the prior art.
To realize above technical purpose, the present invention uses following technical scheme：
A kind of culture medium for streptococcus pneumonia, including following weight portion composition：8 parts of starch, sucrose 6
Part, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, 1 part of the tuber of dwarf lilyturf.
Preferably, also including the cysteine of 1 weight portion.
Preferably, also including the casein of 1 weight portion.
Preferably, also including the glucose of 1 weight portion.
Preferably, the culture medium is fluid nutrient medium.
Preferably, the pH of the culture medium is 6~8；More excellent, its pH is 7.0.
In above technical scheme, the culture medium is to be crushed after each composition of formula ratio is mixed, added water
Arrive, the crushing can be realized using all kinds of crushing material means well-known in the art.
The present invention has screened the nutriment as culture of streptococcus pneumonia base by laboratory facilities extensively, according to lung
Scorching streptococcus own metabolism characteristic and practical effect determine inventive formulation.The formula is conventional in culture medium
Certain Chinese medicine composition is with the addition of on the basis of material, prominent culture effect is surprisingly obtained so that pneumonia
Streptococcus multiplication rate is obviously improved, while being not susceptible to microbiological contamination problem.
Specific embodiment
Specific embodiment of the invention will be below described in detail.In order to avoid excessive unnecessary details,
Be will not be described in detail to belonging to known structure or function in the examples below.In addition to being defined, below
Technology used and scientific terminology have the phase being commonly understood by with those skilled in the art of the invention in embodiment
Same implication.
Embodiment 1
A kind of culture medium for streptococcus pneumonia, is composed of the following components in parts by weight：8 parts of starch, sucrose
6 parts, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, 1 part of the tuber of dwarf lilyturf, cysteine
1 part, 1 part of casein, 1 part of glucose.The culture medium is fluid nutrient medium, and its pH is 7.
Embodiment 2
A kind of culture medium for streptococcus pneumonia, is composed of the following components in parts by weight：8 parts of starch, sucrose
6 parts, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, 1 part of the tuber of dwarf lilyturf, cysteine
1 part, 1 part of glucose.The pH of the culture medium is 6.
Embodiment 3
A kind of culture medium for streptococcus pneumonia, is composed of the following components in parts by weight：8 parts of starch, sucrose
6 parts, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, 1 part of the tuber of dwarf lilyturf, casein 1
Part.The culture medium is fluid nutrient medium, and its pH is 8.
Embodiment 4
A kind of culture medium for streptococcus pneumonia, is composed of the following components in parts by weight：8 parts of starch, sucrose
6 parts, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, 1 part of the tuber of dwarf lilyturf.
Embodiments of the invention have been described in detail above, but the content is only preferable implementation of the invention
Example, is not intended to limit the invention.All any modification, equivalents made in application range of the invention
With improve etc., should be included within the scope of the present invention.
Claims (6)
1. a kind of culture medium for streptococcus pneumonia, it is characterised in that the composition including following weight portion：Form sediment
8 parts of powder, 6 parts of sucrose, 2 parts of potato, 1 part of nicotinic acid, 1 part of folium lycii, 1 part of amethyst, the tuber of dwarf lilyturf 1
Part.
2. culture medium according to claim 1, it is characterised in that also including the cysteine of 1 weight portion.
3. culture medium according to claim 1, it is characterised in that also including the casein of 1 weight portion.
4. culture medium according to claim 1, it is characterised in that also including the glucose of 1 weight portion.
5. culture medium according to claim 1, it is characterised in that the culture medium is fluid nutrient medium.
6. culture medium according to claim 1, it is characterised in that the pH of the culture medium is 6~8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510929439.7A CN106867926A (en) | 2015-12-14 | 2015-12-14 | A kind of culture medium for streptococcus pneumonia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510929439.7A CN106867926A (en) | 2015-12-14 | 2015-12-14 | A kind of culture medium for streptococcus pneumonia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106867926A true CN106867926A (en) | 2017-06-20 |
Family
ID=59238370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510929439.7A Pending CN106867926A (en) | 2015-12-14 | 2015-12-14 | A kind of culture medium for streptococcus pneumonia |
Country Status (1)
| Country | Link |
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| CN (1) | CN106867926A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723337A (en) * | 2017-11-30 | 2018-02-23 | 成都欧林生物科技股份有限公司 | Screen the preparation method of streptococcus pneumonia solid medium |
| CN107746825A (en) * | 2017-11-30 | 2018-03-02 | 成都欧林生物科技股份有限公司 | Beneficial to the culture medium of streptococcus pneumonia fast breeding |
| CN107760635A (en) * | 2017-11-30 | 2018-03-06 | 成都欧林生物科技股份有限公司 | Beneficial to the bacterium culture medium of anti-microbiological contamination |
| CN107858308A (en) * | 2017-11-30 | 2018-03-30 | 成都欧林生物科技股份有限公司 | Bacterium culture medium preparation method for anti-microbiological contamination |
-
2015
- 2015-12-14 CN CN201510929439.7A patent/CN106867926A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723337A (en) * | 2017-11-30 | 2018-02-23 | 成都欧林生物科技股份有限公司 | Screen the preparation method of streptococcus pneumonia solid medium |
| CN107746825A (en) * | 2017-11-30 | 2018-03-02 | 成都欧林生物科技股份有限公司 | Beneficial to the culture medium of streptococcus pneumonia fast breeding |
| CN107760635A (en) * | 2017-11-30 | 2018-03-06 | 成都欧林生物科技股份有限公司 | Beneficial to the bacterium culture medium of anti-microbiological contamination |
| CN107858308A (en) * | 2017-11-30 | 2018-03-30 | 成都欧林生物科技股份有限公司 | Bacterium culture medium preparation method for anti-microbiological contamination |
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Legal Events
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170620 |